Standard name
Human Ortholog
Description 3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds flap DNA, stimulates activity of Rad27p and Dna2p; prevents crossovers between ectopic sequences by removing substrates for Mus81-Mms4 or Rad1-Rad10 cleavage; homolog of human FANCM Fanconi anemia protein that is involved in stabilizing and remodeling blocked replication forks; member of SF2 DExD/H superfamily of helicases; nonsense or missense mutations in FANCM can make people more likely to get cancer

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.07 0.17 0.2 0.21 0.24 0.2 0.2 0.23 0.18 0.2 0.17 0.18 0.26 0.29 0.18 0.15 0.2 0 0.07 0.06 0.05 0.06 0.09
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.16 0.44 0.56 0.38 0.67 0.71 0.65 0.82 0.82 0.83 0.07 0 0.22 0 0 0 0 0 0.05 0 0.05 0.05
Nucleus 0.82 0.67 0.47 0.5 0.47 0.38 0.36 0.39 0.29 0.22 0.23 0.69 0.63 0.38 0.68 0.66 0.61 0.72 0.68 0.51 0.56 0.41 0.25
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Vac/Vac Membrane 0.17 0.18 0.07 0 0.11 0.08 0.06 0 0.06 0.07 0.08 0.13 0.15 0.22 0.17 0.16 0.16 0.18 0.17 0.29 0.28 0.37 0.43
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 2 2 3 8 7 7 7 12 0 0 2 0 0 0 3 4 5 4 3 3
Bud Neck 0 0 1 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0 2 2 2
Bud Site 0 0 0 2 4 4 10 13 9 10 14 0 0 1 0 0 0
Cell Periphery 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1
Cytoplasm 18 33 59 52 66 80 89 128 58 74 69 31 65 74 50 26 28 5 15 24 11 13 23
Endoplasmic Reticulum 3 0 0 0 1 0 0 0 0 0 1 0 0 0 11 12 5 0 0 1 1 0 2
Endosome 2 0 1 0 4 0 2 1 0 0 1 1 2 1 3 4 4 4 2 3 0 1 9
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 1 3 1 0 1 0 0 0
Mitochondria 0 32 132 139 105 276 320 358 267 306 343 12 6 57 8 4 4 5 5 19 2 10 14
Nucleus 220 134 142 124 127 157 162 213 94 81 96 120 157 99 192 114 85 223 156 196 125 97 67
Nuclear Periphery 1 0 0 0 0 0 2 1 0 0 1 0 0 0 1 0 0 0 0 1 1 0 0
Nucleolus 0 0 1 0 0 1 0 3 0 0 2 1 0 0 0 0 2 2 1 0 2 4 7
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 2 1 3 5 1 6 4 2 1 0 3 1 2 1 2 3 3 8 6 9 14
Vac/Vac Membrane 45 36 22 11 31 33 25 22 18 25 35 23 37 58 48 28 22 54 38 113 63 87 116
Unique Cell Count 268 199 301 247 273 410 449 549 324 372 414 173 249 258 284 172 140 309 230 386 226 236 271
Labelled Cell Count 289 236 360 331 343 560 620 752 457 505 575 188 271 293 318 190 155 309 230 386 226 236 271


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 4.1 3.7 3.7 3.9 3.4 3.3 3.4 2.8 2.8 2.7 4.6 4.7 4.0 6.3 6.8 7.3 4.8 4.7 5.1
Std Deviation (1e-4) 0.8 0.7 1.2 1.6 1.6 1.4 1.5 1.5 1.3 0.8 0.9 1.2 1.1 1.0 1.5 1.6 2.3 1.5 1.3 1.6
Intensity Change (Log2) -0.01 0.06 -0.15 -0.18 -0.13 -0.4 -0.42 -0.45 0.31 0.32 0.09 0.75 0.86 0.97 0.37 0.34 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 3.6 3.1 2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.456 1.2714 1.2148 0.8313 0.9167 1.0516 2.0005 2.4442 2.3177 1.9071 2.8659 2.2121 2.7644 3.9394 2.9383 2.7882 2.6441 3.1586
Actin 0.064 0.0006 0.0161 0.0001 0.0136 0.0017 0.0169 0.0001 0.0224 0.0075 0.0254 0.0011 0.0034 0.0006 0.0056 0.0004 0.0005 0.0002
Bud 0.0009 0.0002 0.001 0.0002 0.0005 0.0016 0.0007 0.0002 0.0004 0.0011 0.0021 0.0001 0.0011 0.0001 0.0004 0.0001 0.0001 0
Bud Neck 0.0058 0.0009 0.0011 0.001 0.0015 0.0066 0.0022 0.0006 0.001 0.003 0.0036 0.0035 0.0003 0.0006 0.0022 0.0008 0.0017 0.0026
Bud Periphery 0.0022 0.0003 0.0018 0.0014 0.002 0.0047 0.0006 0.0002 0.0005 0.0012 0.0019 0.0001 0.0005 0.0001 0.0009 0.0001 0.0001 0
Bud Site 0.0048 0.0015 0.0169 0.0007 0.001 0.0011 0.0059 0.0015 0.0058 0.0115 0.0142 0.0002 0.0005 0.0005 0.0028 0.0002 0.0004 0.0001
Cell Periphery 0.0005 0.0002 0.0003 0.0006 0.0004 0.0002 0.0002 0.0001 0.0002 0.0004 0.0002 0 0.0001 0.0001 0.0001 0 0.0001 0
Cytoplasm 0.0384 0.0787 0.0401 0.0566 0.0298 0.0312 0.0521 0.0275 0.0313 0.0642 0.0207 0.0258 0.0633 0.0372 0.0467 0.0213 0.0486 0.0308
Cytoplasmic Foci 0.0158 0.004 0.007 0.0008 0.0023 0.0059 0.0195 0.001 0.0074 0.0335 0.0392 0.0024 0.0019 0.0021 0.0023 0.0003 0.0013 0.0008
Eisosomes 0.0011 0 0.0002 0 0.0007 0 0.0004 0 0.0002 0.0001 0.0003 0 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0082 0.0035 0.0061 0.0017 0.0069 0.0024 0.0026 0.0018 0.0068 0.0027 0.0065 0.0012 0.003 0.0024 0.0018 0.0009 0.0026 0.0007
Endosome 0.0261 0.0034 0.0168 0.0059 0.0226 0.0072 0.0162 0.0021 0.0077 0.0272 0.021 0.0068 0.0024 0.0018 0.0053 0.0008 0.0027 0.0011
Golgi 0.0122 0.0002 0.0043 0.0001 0.0058 0.0018 0.0045 0.0001 0.0035 0.0078 0.0206 0.0019 0.001 0.0001 0.0009 0 0.0001 0
Lipid Particles 0.0297 0.0009 0.013 0.0005 0.0061 0.0085 0.0068 0.0007 0.0025 0.017 0.0063 0.0028 0.0026 0.0002 0.0022 0 0.0002 0.0001
Mitochondria 0.0124 0.0007 0.0085 0.0017 0.0275 0.0013 0.0029 0.0008 0.0033 0.0049 0.0076 0.0034 0.0014 0.0004 0.0014 0.0011 0.0005 0.0003
None 0.0915 0.0469 0.0662 0.0294 0.0256 0.038 0.1181 0.0844 0.0668 0.0749 0.1181 0.0761 0.1005 0.0558 0.0817 0.0384 0.0621 0.0662
Nuclear Periphery 0.0334 0.0209 0.0253 0.0098 0.0375 0.0152 0.0189 0.0165 0.024 0.0152 0.0159 0.013 0.0104 0.0272 0.0178 0.0123 0.0259 0.0066
Nucleolus 0.0085 0.0101 0.008 0.0082 0.0086 0.017 0.0099 0.0124 0.007 0.0081 0.0065 0.0167 0.0045 0.0069 0.0101 0.0074 0.0093 0.0132
Nucleus 0.5697 0.7982 0.732 0.8188 0.7692 0.8178 0.6452 0.817 0.7691 0.5838 0.6272 0.8006 0.7888 0.8045 0.779 0.8483 0.8114 0.8218
Peroxisomes 0.023 0.0003 0.006 0.0001 0.0014 0.0027 0.0071 0.0001 0.0059 0.0516 0.0099 0.0027 0.0004 0.0001 0.0033 0.0001 0.0001 0
Punctate Nuclear 0.0441 0.0217 0.021 0.0314 0.0316 0.0329 0.0655 0.0299 0.0322 0.0744 0.0509 0.0404 0.0116 0.057 0.0268 0.0668 0.0283 0.0545
Vacuole 0.0057 0.0059 0.0057 0.0265 0.0027 0.0018 0.0027 0.0022 0.0016 0.0087 0.0012 0.0008 0.0019 0.0019 0.0057 0.0004 0.0035 0.0007
Vacuole Periphery 0.0021 0.0007 0.0024 0.0045 0.0029 0.0004 0.001 0.0006 0.0006 0.0012 0.0008 0.0003 0.0003 0.0004 0.0029 0.0002 0.0007 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.0304 9.9233 7.1181 9.6405 9.3551 5.9171 9.8662 8.9163 8.8961 9.6984
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1835 97 1381 928 1704 1749 139 152 3539 1846 1520 1080

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 603.60 783.78 885.71 876.54 678.46 748.10 988.04 915.95 639.64 749.97 895.07 882.09
Standard Deviation 76.90 103.69 101.57 124.99 79.25 95.16 113.39 114.48 86.54 95.95 106.86 124.32
Intensity Change Log 2 0.376856 0.553242 0.538227 0.140967 0.542306 0.433005 0.256840 0.547465 0.483501

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000142 0.000712 0.000265 0.004137 0.000049 0.000195 0.000637 0.000696 0.000097 0.000222 0.000299 0.003653
Bud Neck 0.005343 0.002440 0.004807 0.014675 0.002323 0.006629 0.003219 0.005593 0.003889 0.006409 0.004662 0.013397
Bud Site 0.001079 0.000970 0.004394 0.024134 0.000417 0.005606 0.000780 0.004764 0.000760 0.005362 0.004064 0.021408
Cell Periphery 0.000153 0.000142 0.000069 0.000208 0.000110 0.000152 0.000088 0.000132 0.000132 0.000151 0.000071 0.000197
Cytoplasm 0.291535* 0.087634 0.020247 0.075123 0.149933* 0.142570* 0.006617 0.058010 0.223355* 0.139683* 0.019001 0.072714
Cytoplasmic Foci 0.056605 0.024480 0.000226 0.006431 0.022876 0.026748 0.000212 0.000467 0.040365 0.026629 0.000225 0.005592
Eisosomes 0.000056 0.000075 0.000015 0.000031 0.000027 0.000039 0.000047 0.000029 0.000042 0.000041 0.000018 0.000031
Endoplasmic Reticulum 0.000747 0.003181 0.004890 0.008097 0.000923 0.001707 0.012568 0.010223 0.000832 0.001784 0.005592 0.008396
Endosome 0.001534 0.002426 0.000396 0.005941 0.000804 0.004018 0.000590 0.000704 0.001182 0.003934 0.000414 0.005204
Golgi 0.000442 0.000169 0.000025 0.006827 0.000359 0.001391 0.000042 0.000054 0.000402 0.001327 0.000027 0.005874
Lipid Particles 0.005652 0.005155 0.000243 0.000510 0.003418 0.003669 0.000608 0.000477 0.004576 0.003747 0.000276 0.000505
Mitochondria 0.002478 0.002927 0.000797 0.010161 0.001890 0.005331 0.000580 0.001027 0.002195 0.005204 0.000777 0.008875
Mitotic Spindle 0.000553 0.000079 0.000847 0.029768 0.000655 0.009340 0.000117 0.002893 0.000602 0.008853 0.000780 0.025985
None 0.014977 0.004627 0.001679 0.015030 0.010106 0.008280 0.001491 0.005474 0.012631 0.008088 0.001662 0.013685
Nuclear Periphery 0.000832 0.001004 0.001177 0.002396 0.001036 0.001609 0.001090 0.001730 0.000930 0.001577 0.001169 0.002302
Nuclear Periphery Foci 0.000362 0.000563 0.000159 0.000696 0.000250 0.000506 0.000266 0.000465 0.000308 0.000509 0.000168 0.000663
Nucleolus 0.002848 0.002168 0.000606 0.001287 0.003033 0.004882 0.001192 0.000853 0.002937 0.004740 0.000660 0.001226
Nucleus 0.602701* 0.839197* 0.953561* 0.765692* 0.797079* 0.764334* 0.957239* 0.891499* 0.696292* 0.768268* 0.953898* 0.783398*
Peroxisomes 0.002748 0.003139 0.000114 0.003161 0.001696 0.003021 0.000150 0.000096 0.002242 0.003027 0.000117 0.002730
Vacuole 0.008997 0.017095 0.005391 0.021271 0.002861 0.009283 0.012369 0.014675 0.006042 0.009693 0.006029 0.020342
Vacuole Periphery 0.000217 0.001818 0.000092 0.004425 0.000156 0.000693 0.000096 0.000139 0.000188 0.000752 0.000092 0.003822

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.13 -2.62 -3.09 -1.80 -2.85 -5.40 -7.37 -6.96 -6.13 -1.98 -2.69 -6.17 -3.45 -3.24 -3.00
Bud Neck 2.67 -0.72 -7.51 -9.24 -7.39 -4.81 -3.92 -5.08 -2.27 -3.11 -2.84 -2.91 -9.33 -6.96 -7.72
Bud Site -0.03 -2.81 -5.79 -5.74 -3.41 -4.83 -4.53 -1.99 -0.90 -1.76 -4.52 -3.11 -6.24 -3.42 -3.66
Cell Periphery 0.16 4.14 -0.68 -0.59 -7.58 -2.23 0.47 -2.39 -0.34 -2.52 -1.06 4.89 -2.33 -0.72 -8.07
Cytoplasm 12.88 46.09 35.46 2.25 -7.71 0.98 27.07 12.41 11.82 -4.31 13.87 48.34 33.45 16.08 -8.40
Cytoplasmic Foci 4.65 22.09 20.50 3.52 -2.84 -2.02 13.87 13.64 15.61 -2.25 5.33 25.65 22.83 14.32 -2.90
Eisosomes -1.51 11.85 1.42 1.85 -13.04 -4.55 -4.39 -5.66 -3.27 0.37 -0.08 9.71 -3.30 -2.84 -12.31
Endoplasmic Reticulum -3.31 -15.77 -19.56 -9.44 -9.98 -4.45 -8.26 -9.21 -8.70 -0.61 -5.87 -17.34 -21.50 -19.09 -9.84
Endosome -0.80 6.49 -1.88 -0.21 -4.17 -5.85 0.35 -2.14 4.99 -2.43 -5.22 6.51 -2.64 2.30 -4.34
Golgi 2.36 4.08 -1.88 -2.20 -2.35 -2.23 2.24 1.62 2.84 -2.43 -2.15 4.53 -1.88 -0.67 -2.37
Lipid Particles 0.30 7.65 6.91 2.33 -5.25 -0.70 5.19 5.05 8.61 -0.52 1.15 9.71 8.59 9.34 -5.45
Mitochondria -0.11 3.14 -1.68 -0.92 -2.96 -3.31 2.13 0.40 3.89 -2.70 -3.24 3.45 -1.84 0.53 -3.06
Mitotic Spindle 1.31 -1.50 -6.58 -6.75 -6.22 -4.99 0.02 -2.60 -0.04 -2.60 -5.07 -1.47 -6.89 -3.76 -6.51
None 5.87 9.83 3.16 -2.69 -5.87 1.30 8.03 1.38 0.53 -2.29 3.35 13.06 2.74 -0.50 -6.29
Nuclear Periphery -1.16 -5.96 -14.81 -10.70 -10.65 -3.84 -2.91 -4.93 -3.47 -3.89 -4.78 -5.61 -15.29 -9.41 -11.24
Nuclear Periphery Foci -0.88 2.32 -5.43 -1.86 -6.79 -3.12 -1.38 -3.70 -2.27 -2.63 -2.76 1.68 -6.75 -3.76 -7.39
Nucleolus 1.64 5.40 1.74 -0.07 -5.35 -2.64 4.40 2.42 4.50 -1.25 -2.94 7.78 2.73 4.61 -5.60
Nucleus -9.41 -45.87 -18.21 1.26 16.23 3.95 -12.62 -2.49 -4.44 4.92 -8.30 -43.55 -12.13 -4.14 17.01
Peroxisomes -0.18 6.21 2.39 1.24 -1.72 -2.68 4.54 4.56 7.88 0.12 -1.74 7.71 2.34 3.46 -1.72
Vacuole -1.92 1.02 -10.47 -2.05 -10.80 -8.68 -3.25 -5.09 -4.39 -2.16 -4.61 -3.09 -12.46 -10.84 -10.95
Vacuole Periphery -1.07 3.95 -2.44 0.10 -2.65 -4.90 1.85 -0.73 4.61 -2.36 -4.47 3.84 -2.49 -1.23 -2.67
Standard name
Human Ortholog
Description 3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds flap DNA, stimulates activity of Rad27p and Dna2p; prevents crossovers between ectopic sequences by removing substrates for Mus81-Mms4 or Rad1-Rad10 cleavage; homolog of human FANCM Fanconi anemia protein that is involved in stabilizing and remodeling blocked replication forks; member of SF2 DExD/H superfamily of helicases; nonsense or missense mutations in FANCM can make people more likely to get cancer
Localization
Cell Percentages nucleus (45%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Mph1

Mph1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mph1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available