Standard name | |
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Human Ortholog | |
Description | Essential component of the outer kinetochore MIND complex; joins kinetochore subunits contacting DNA to those contacting microtubules; phosphorylation promotes interaction between outer and inner kinetochore proteins; kinetochore receptor for monopolin, via interaction with Csm1p; essential for both meiotic and mitotic chromosome segregation; MIND complex consists of Mtw1p, Nnf1p, Nsl1p and Dsn1p; phosphorylated by monopolin subunit, Hrr25p and Aurora kinase, Ipl1p; modified by sumoylation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 2 | 3 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 0 | 1 | 2 | 2 | 0 | 4 | 9 | – | 0 | 5 | 8 | 5 | 0 | 0 | 0 | 4 | 5 | 6 | 2 | 3 | 0 | 0 | 1 | 0 |
Bud Neck | 2 | 13 | 3 | 4 | 7 | 4 | 10 | – | 5 | 6 | 5 | 8 | 3 | 4 | 3 | 10 | 7 | 3 | 0 | 4 | 1 | 0 | 0 | 0 |
Bud Site | 4 | 3 | 1 | 1 | 0 | 6 | 3 | – | 9 | 6 | 11 | 3 | 0 | 0 | 0 | 9 | 4 | 6 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 5 | 8 | 2 | 13 | 17 | 6 | 15 | – | 11 | 11 | 10 | 9 | 6 | 32 | 34 | 17 | 28 | 20 | 4 | 1 | 0 | 0 | 0 | 3 |
Endoplasmic Reticulum | 0 | 2 | 1 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 4 | 0 | 0 | 0 | 0 | 1 | 1 |
Endosome | 12 | 24 | 7 | 1 | 4 | 1 | 0 | – | 1 | 1 | 3 | 3 | 6 | 20 | 27 | 37 | 51 | 55 | 3 | 11 | 11 | 3 | 3 | 4 |
Golgi | 1 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 9 | 11 | 1 | 4 | 1 | 2 | 7 | – | 9 | 10 | 34 | 11 | 1 | 0 | 0 | 22 | 3 | 8 | 0 | 3 | 2 | 0 | 0 | 3 |
Nucleus | 3 | 9 | 6 | 12 | 4 | 8 | 5 | – | 9 | 9 | 6 | 10 | 3 | 7 | 5 | 22 | 11 | 16 | 0 | 4 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 2 | – | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 119 | 145 | 49 | 48 | 44 | 43 | 72 | – | 52 | 59 | 80 | 87 | 60 | 67 | 49 | 188 | 218 | 156 | 3 | 31 | 3 | 3 | 5 | 5 |
Peroxisomes | 3 | 12 | 0 | 0 | 1 | 1 | 3 | – | 2 | 0 | 10 | 4 | 0 | 3 | 4 | 8 | 6 | 5 | 1 | 4 | 2 | 0 | 0 | 1 |
SpindlePole | 167 | 214 | 187 | 107 | 157 | 115 | 221 | – | 118 | 131 | 143 | 182 | 94 | 147 | 138 | 333 | 339 | 309 | 182 | 228 | 174 | 61 | 82 | 139 |
Vac/Vac Membrane | 2 | 2 | 1 | 1 | 1 | 0 | 1 | – | 0 | 2 | 1 | 0 | 0 | 2 | 1 | 6 | 1 | 6 | 0 | 2 | 0 | 0 | 0 | 0 |
Unique Cell Count | 196 | 285 | 198 | 136 | 182 | 134 | 257 | 151 | 167 | 197 | 215 | 116 | 194 | 189 | 413 | 407 | 368 | 204 | 301 | 199 | 71 | 103 | 170 | |
Labelled Cell Count | 328 | 445 | 261 | 193 | 236 | 192 | 352 | 216 | 240 | 312 | 323 | 173 | 282 | 262 | 660 | 678 | 595 | 204 | 301 | 199 | 71 | 103 | 170 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.0 | 6.1 | 6.2 | 5.8 | 6.6 | 5.0 | 5.5 | – | 4.6 | 4.7 | 4.5 | 4.6 | 6.0 | 6.5 | 6.1 | 7.2 | 7.6 | 7.1 | 4.8 | 5.9 | 6.1 |
Std Deviation (1e-4) | 1.0 | 1.0 | 1.2 | 1.6 | 1.8 | 1.2 | 1.5 | – | 1.7 | 1.2 | 1.6 | 1.4 | 1.1 | 1.6 | 1.8 | 1.3 | 1.5 | 1.3 | 0.4 | 1.5 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.1 | 0.09 | -0.3 | -0.16 | – | -0.42 | -0.41 | -0.45 | -0.41 | -0.03 | 0.07 | -0.01 | 0.23 | 0.29 | 0.2 | -0.37 | -0.08 | -0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 1.5 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3.7 | 3.7 | 0 | 2.7 | – | 3.1 | 2.9 | 2.4 | 2.0 | 0 | 5.4 | 5.7 | 2.1 | 3.1 | 2.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | -0.8 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2.6 | 3.7 | 2.4 | 3.5 | 4.1 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 0 | 0 |
Nucleus | 2.3 | 0 | 1.3 | 0 | – | 1.3 | 1.1 | 0 | 0.9 | 0 | 0.3 | -0.2 | 1.3 | -0.2 | 0.8 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2.1 | -0.1 | 1.5 | 0.8 | – | 0 | 0 | 0 | 0 | 4.8 | 2.1 | 0.3 | 4.9 | 6.7 | 4.2 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | -4.4 | -2.7 | -2.7 | -2.9 | – | 0 | 0 | 0 | 0 | -3.7 | -5.2 | -5.7 | -4.5 | -3.8 | -3.6 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.2448 | 0.5351 | 0.6954 | 0.9406 | 1.5031 | 0.6323 | 1.393 | 0.9409 | 1.1055 | 1.1823 | 1.5391 | 1.0266 | 1.0499 | 0.7226 | 0.772 | 1.3018 | 0.661 | 0.3181 |
Actin | 0.0193 | 0.0001 | 0.0058 | 0.0107 | 0.0032 | 0 | 0.0137 | 0 | 0.0089 | 0.0031 | 0.0033 | 0.0003 | 0.0288 | 0 | 0.0217 | 0.0041 | 0.0273 | 0.0048 |
Bud | 0.0006 | 0.0005 | 0.0001 | 0.0006 | 0.0018 | 0 | 0.0006 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0005 | 0.0001 | 0.0002 | 0.0019 | 0.0001 | 0.0004 |
Bud Neck | 0.0088 | 0.0012 | 0.0006 | 0.0014 | 0.0009 | 0.0004 | 0.0017 | 0.0016 | 0.0006 | 0.0224 | 0.0003 | 0.0002 | 0.0018 | 0.0005 | 0.0006 | 0.0116 | 0.0003 | 0.0004 |
Bud Periphery | 0.0011 | 0.0005 | 0.0001 | 0.0015 | 0.0038 | 0 | 0.001 | 0.0001 | 0.0003 | 0 | 0.0002 | 0 | 0.0006 | 0.0001 | 0.0002 | 0.0019 | 0.0002 | 0.0008 |
Bud Site | 0.0143 | 0.0034 | 0.0013 | 0.0014 | 0.0064 | 0.0001 | 0.008 | 0.0018 | 0.0026 | 0.0001 | 0.0006 | 0.0001 | 0.0069 | 0.0003 | 0.0015 | 0.0112 | 0.0005 | 0.0004 |
Cell Periphery | 0.0013 | 0.0001 | 0 | 0.0002 | 0.0008 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0.0004 | 0 | 0.0001 |
Cytoplasm | 0.0066 | 0.0009 | 0.0029 | 0.0061 | 0.0032 | 0 | 0.0052 | 0.0002 | 0.0006 | 0.0003 | 0.0006 | 0.0003 | 0.0028 | 0.0018 | 0.0003 | 0.0118 | 0.0002 | 0.0008 |
Cytoplasmic Foci | 0.0288 | 0.0036 | 0.0204 | 0.0288 | 0.0634 | 0.0025 | 0.0327 | 0.0049 | 0.0138 | 0.0137 | 0.1011 | 0.0038 | 0.0441 | 0.0114 | 0.0169 | 0.0254 | 0.0382 | 0.0105 |
Eisosomes | 0.0003 | 0 | 0 | 0.0001 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0058 | 0.0001 | 0.0012 | 0.0037 | 0.0016 | 0 | 0.0017 | 0 | 0.001 | 0.0005 | 0.0005 | 0.0003 | 0.0027 | 0.0001 | 0.0002 | 0.002 | 0.0001 | 0.0006 |
Endosome | 0.0162 | 0.0007 | 0.008 | 0.0383 | 0.0405 | 0.0002 | 0.0136 | 0.0004 | 0.0049 | 0.0035 | 0.0184 | 0.0082 | 0.0095 | 0.0015 | 0.0034 | 0.0555 | 0.0031 | 0.0077 |
Golgi | 0.0042 | 0.0002 | 0.0022 | 0.0142 | 0.0202 | 0.0001 | 0.0066 | 0.0002 | 0.0047 | 0.0025 | 0.0155 | 0.0008 | 0.007 | 0.0003 | 0.0035 | 0.0168 | 0.0049 | 0.0026 |
Lipid Particles | 0.0283 | 0.0008 | 0.0234 | 0.0308 | 0.1253 | 0.0015 | 0.0288 | 0.0011 | 0.0151 | 0.0353 | 0.0665 | 0.0067 | 0.0247 | 0.0078 | 0.0087 | 0.0225 | 0.0833 | 0.0062 |
Mitochondria | 0.0025 | 0.0002 | 0.0071 | 0.0088 | 0.0158 | 0 | 0.0069 | 0 | 0.0074 | 0.0002 | 0.0036 | 0.0001 | 0.0045 | 0.0008 | 0.0004 | 0.0017 | 0.0005 | 0.003 |
None | 0.0009 | 0.0021 | 0.0015 | 0.0617 | 0.0009 | 0 | 0.0064 | 0.0003 | 0.0005 | 0.0005 | 0.0003 | 0.0001 | 0.0023 | 0.0019 | 0.0002 | 0.0014 | 0.0001 | 0.001 |
Nuclear Periphery | 0.0105 | 0.0007 | 0.0031 | 0.0113 | 0.0227 | 0.0012 | 0.0066 | 0.0005 | 0.0026 | 0.0027 | 0.0007 | 0.001 | 0.0051 | 0.0006 | 0.0005 | 0.0031 | 0.0019 | 0.001 |
Nucleolus | 0.0249 | 0.1045 | 0.0437 | 0.0466 | 0.0184 | 0.0401 | 0.0293 | 0.0874 | 0.024 | 0.0043 | 0.0039 | 0.0189 | 0.0227 | 0.068 | 0.0439 | 0.0386 | 0.0106 | 0.0039 |
Nucleus | 0.0073 | 0.0632 | 0.0258 | 0.0296 | 0.0031 | 0.0155 | 0.0106 | 0.0347 | 0.0122 | 0.0033 | 0.0004 | 0.005 | 0.0071 | 0.0258 | 0.0103 | 0.0113 | 0.0006 | 0.0013 |
Peroxisomes | 0.015 | 0.0005 | 0.0098 | 0.0035 | 0.0324 | 0.0002 | 0.0121 | 0.0002 | 0.0063 | 0.0028 | 0.0584 | 0.0016 | 0.0324 | 0.0038 | 0.004 | 0.0066 | 0.043 | 0.0009 |
Punctate Nuclear | 0.7998 | 0.8158 | 0.8419 | 0.6933 | 0.6238 | 0.9378 | 0.8068 | 0.8661 | 0.8934 | 0.9044 | 0.725 | 0.9521 | 0.7946 | 0.8749 | 0.8833 | 0.7634 | 0.785 | 0.9527 |
Vacuole | 0.0026 | 0.0009 | 0.0007 | 0.0039 | 0.0091 | 0.0001 | 0.0046 | 0.0004 | 0.0004 | 0.0001 | 0.0003 | 0.0003 | 0.0007 | 0.0003 | 0.0002 | 0.0075 | 0 | 0.0005 |
Vacuole Periphery | 0.0008 | 0.0001 | 0.0005 | 0.0034 | 0.0027 | 0 | 0.0024 | 0 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0006 | 0 | 0.0001 | 0.0012 | 0 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 18.167 | 17.6647 | 9.7253 | 9.4779 | 19.8352 | 19.1125 | 17.4287 | 17.1101 | 14.0059 | 15.3519 |
Translational Efficiency | 0.8434 | 1.1983 | 1.0174 | 0.912 | 0.735 | 0.7406 | 0.9276 | 0.886 | 0.8426 | 0.9209 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
419 | 1232 | 721 | 135 | 1819 | 1485 | 262 | 550 | 2238 | 2717 | 983 | 685 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 568.52 | 790.48 | 949.61 | 933.41 | 754.02 | 736.42 | 979.51 | 882.82 | 719.29 | 760.93 | 957.58 | 892.79 |
Standard Deviation | 84.36 | 105.89 | 110.44 | 132.37 | 90.63 | 95.44 | 109.59 | 124.66 | 115.08 | 103.86 | 111.00 | 127.81 |
Intensity Change Log 2 | — | 0.475518 | 0.740124 | 0.715300 | — | -0.034074 | 0.377457 | 0.227517 | — | 0.207294 | 0.544632 | 0.457636 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000657 | 0.000506 | 0.000052 | 0.001219 | 0.000254 | 0.000733 | 0.000350 | 0.000516 | 0.000329 | 0.000630 | 0.000131 | 0.000655 |
Bud Neck | 0.005400 | 0.004771 | 0.000795 | 0.001378 | 0.005125 | 0.006237 | 0.002323 | 0.002450 | 0.005176 | 0.005572 | 0.001202 | 0.002239 |
Bud Site | 0.015658 | 0.006022 | 0.000817 | 0.015778 | 0.004969 | 0.005617 | 0.003850 | 0.023500 | 0.006970 | 0.005801 | 0.001626 | 0.021978 |
Cell Periphery | 0.000441 | 0.000132 | 0.000008 | 0.000059 | 0.000195 | 0.000126 | 0.000049 | 0.000079 | 0.000241 | 0.000129 | 0.000019 | 0.000075 |
Cytoplasm | 0.038855 | 0.017592 | 0.000085 | 0.010431 | 0.017295 | 0.020156 | 0.007482 | 0.019075 | 0.021332 | 0.018994 | 0.002057 | 0.017371 |
Cytoplasmic Foci | 0.164317 | 0.038604 | 0.000562 | 0.024000 | 0.051445 | 0.052055 | 0.005415 | 0.008513 | 0.072577 | 0.045956 | 0.001856 | 0.011565 |
Eisosomes | 0.000424 | 0.000078 | 0.000005 | 0.000010 | 0.000122 | 0.000071 | 0.000035 | 0.000009 | 0.000179 | 0.000074 | 0.000013 | 0.000009 |
Endoplasmic Reticulum | 0.002266 | 0.001684 | 0.000133 | 0.000123 | 0.001395 | 0.001321 | 0.001385 | 0.000232 | 0.001558 | 0.001486 | 0.000467 | 0.000210 |
Endosome | 0.012318 | 0.014344 | 0.000235 | 0.012505 | 0.008373 | 0.015882 | 0.005348 | 0.004873 | 0.009112 | 0.015184 | 0.001598 | 0.006377 |
Golgi | 0.025820 | 0.013741 | 0.000279 | 0.008638 | 0.007763 | 0.011113 | 0.000902 | 0.007226 | 0.011143 | 0.012305 | 0.000445 | 0.007504 |
Lipid Particles | 0.027424 | 0.013720 | 0.000166 | 0.000366 | 0.009337 | 0.016563 | 0.001017 | 0.000345 | 0.012723 | 0.015274 | 0.000393 | 0.000349 |
Mitochondria | 0.001733 | 0.005910 | 0.000040 | 0.000295 | 0.001379 | 0.002522 | 0.000179 | 0.002913 | 0.001445 | 0.004058 | 0.000077 | 0.002397 |
Mitotic Spindle | 0.222060* | 0.688970* | 0.983429* | 0.904303* | 0.631888* | 0.659189* | 0.888512* | 0.900367* | 0.555160* | 0.672693* | 0.958130* | 0.901143* |
None | 0.043708 | 0.003601 | 0.000056 | 0.000672 | 0.009326 | 0.005065 | 0.000731 | 0.001109 | 0.015763 | 0.004401 | 0.000236 | 0.001022 |
Nuclear Periphery | 0.000358 | 0.000398 | 0.000097 | 0.000093 | 0.000541 | 0.000516 | 0.000385 | 0.000564 | 0.000507 | 0.000463 | 0.000173 | 0.000471 |
Nuclear Periphery Foci | 0.184952 | 0.089633 | 0.003341 | 0.005388 | 0.116341 | 0.061934 | 0.011216 | 0.002166 | 0.129186 | 0.074494 | 0.005440 | 0.002801 |
Nucleolus | 0.147310 | 0.043188 | 0.006373 | 0.004252 | 0.086726 | 0.075933 | 0.015770 | 0.002431 | 0.098069 | 0.061085 | 0.008878 | 0.002790 |
Nucleus | 0.039922 | 0.027014 | 0.002328 | 0.002098 | 0.025060 | 0.040843 | 0.042136 | 0.005462 | 0.027842 | 0.034572 | 0.012938 | 0.004799 |
Peroxisomes | 0.064940 | 0.026195 | 0.000884 | 0.001478 | 0.021430 | 0.021624 | 0.004262 | 0.003137 | 0.029576 | 0.023697 | 0.001784 | 0.002810 |
Vacuole | 0.001293 | 0.003154 | 0.000294 | 0.006487 | 0.000874 | 0.002123 | 0.008493 | 0.010113 | 0.000952 | 0.002591 | 0.002479 | 0.009399 |
Vacuole Periphery | 0.000145 | 0.000743 | 0.000020 | 0.000426 | 0.000162 | 0.000376 | 0.000161 | 0.004919 | 0.000159 | 0.000542 | 0.000058 | 0.004033 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.79 | 1.81 | -1.11 | -1.34 | -1.62 | -2.52 | -4.88 | -2.69 | -0.73 | 3.45 | -3.03 | -4.24 | -2.78 | -1.41 | 0.23 |
Bud Neck | -0.22 | -0.19 | -2.43 | -2.42 | -2.38 | -2.13 | -3.48 | -2.29 | -0.81 | 1.24 | -1.80 | -1.98 | -3.22 | -2.27 | -1.70 |
Bud Site | 2.13 | 2.32 | -2.84 | -3.70 | -3.78 | -2.01 | -5.75 | -6.00 | -5.65 | -2.37 | -0.15 | -2.73 | -6.67 | -6.68 | -5.78 |
Cell Periphery | 6.45 | 8.82 | 6.62 | 1.70 | -2.15 | 3.25 | 0.24 | 4.11 | 2.27 | 3.30 | 6.21 | 10.35 | 6.73 | 2.93 | -0.58 |
Cytoplasm | 3.16 | 5.77 | 1.87 | -0.72 | -3.10 | -1.49 | -1.88 | -4.25 | -3.57 | -2.35 | 0.34 | 4.36 | -3.64 | -3.77 | -5.28 |
Cytoplasmic Foci | 10.76 | 14.16 | 10.39 | 2.13 | -2.26 | 0.68 | 10.23 | 9.92 | 8.87 | -0.64 | 7.22 | 19.51 | 13.85 | 8.87 | -4.23 |
Eisosomes | 3.23 | 4.68 | 4.55 | 10.17 | -0.92 | 8.45 | 4.67 | 25.34 | 19.21 | 6.87 | 6.48 | 11.54 | 13.58 | 23.17 | 4.72 |
Endoplasmic Reticulum | 2.80 | 9.09 | 2.61 | 1.47 | -1.23 | 1.21 | -4.30 | 7.04 | 5.88 | 5.80 | 0.89 | 1.01 | 6.85 | 6.22 | 3.59 |
Endosome | 1.54 | 9.47 | 0.65 | 0.03 | -2.28 | -1.82 | -0.23 | 3.44 | 4.42 | 2.02 | -1.28 | 8.00 | 2.89 | 3.58 | -2.19 |
Golgi | 6.94 | 11.77 | 7.49 | 2.92 | -1.24 | 1.17 | 13.38 | 4.56 | 3.86 | -0.26 | 4.51 | 20.55 | 7.33 | 4.90 | -1.44 |
Lipid Particles | 4.42 | 7.79 | 7.51 | 8.22 | -2.61 | -1.18 | 6.05 | 10.68 | 6.62 | 5.13 | 0.94 | 11.99 | 12.26 | 10.51 | 1.24 |
Mitochondria | -1.40 | 5.45 | 4.01 | 2.45 | -1.48 | -2.21 | 1.47 | -0.91 | -0.23 | -1.20 | -2.42 | 4.23 | -0.65 | 0.80 | -1.45 |
Mitotic Spindle | -22.08* | -38.78* | -17.49 | -4.89 | 3.62 | -4.41 | -6.21 | -16.27 | -12.23 | -4.04 | -11.77 | -29.12 | -21.76 | -13.37 | 2.30 |
None | 6.55 | 7.17 | 6.94 | 2.32 | -1.37 | 1.95 | 6.61 | 5.19 | 3.65 | -0.76 | 6.64 | 9.84 | 8.46 | 3.95 | -2.40 |
Nuclear Periphery | -1.65 | 2.91 | -1.99 | -1.54 | -2.67 | 0.87 | -3.46 | -1.43 | -1.56 | 0.95 | 0.99 | 0.83 | -1.80 | -1.96 | -1.98 |
Nuclear Periphery Foci | 5.91 | 15.52 | 15.56 | 17.06* | 1.49 | 8.10 | 13.88 | 26.22* | 18.12* | 5.03 | 8.78 | 26.72 | 30.36 | 25.37* | 4.68 |
Nucleolus | 7.77 | 10.27 | 10.56 | 5.71 | 2.30 | 0.96 | 8.81 | 19.63 | 16.60 | 4.97 | 5.88 | 14.68 | 22.09 | 18.31 | 6.72 |
Nucleus | 0.42 | 1.66 | 0.37 | 0.14 | -0.64 | -3.19 | -4.82 | -1.26 | 0.84 | 4.24 | -2.64 | -3.44 | -0.98 | 0.61 | 2.06 |
Peroxisomes | 7.11 | 12.31 | 12.04 | 10.80 | -0.12 | 1.11 | 5.18 | 14.13 | 10.84 | 4.39 | 4.06 | 17.12 | 18.11 | 15.24 | 2.03 |
Vacuole | -2.77 | -1.94 | -3.49 | -3.44 | -3.46 | -2.55 | -4.63 | -5.59 | -5.51 | -0.30 | -4.39 | -4.88 | -6.56 | -6.41 | -4.85 |
Vacuole Periphery | -2.37 | 1.50 | -1.54 | 0.04 | -1.69 | -4.19 | -5.48 | -2.09 | -1.98 | -1.80 | -3.51 | -3.67 | -2.14 | -1.92 | -2.06 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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