Standard name | |
---|---|
Human Ortholog | |
Description | GTPase activating factor for Rsr1p/Bud1p; plays a role in spindle position checkpoint distinct from its role in bud site selection; required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types; contains two PH-like domains |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 11 | 10 | 31 | 24 | 36 | 45 | 32 | 36 | 19 | – | 20 | 17 | 36 | 76 | 68 | 3 | 8 | 7 | 9 | 8 | 30 | 37 | 61 | 56 |
Bud | 9 | 7 | 30 | 31 | 48 | 50 | 46 | 57 | 42 | – | 66 | 63 | 18 | 36 | 39 | 2 | 6 | 3 | 5 | 5 | 17 | 27 | 61 | 86 |
Bud Neck | 3 | 1 | 16 | 16 | 39 | 27 | 35 | 30 | 18 | – | 32 | 30 | 14 | 15 | 23 | 0 | 3 | 0 | 26 | 32 | 63 | 74 | 121 | 113 |
Bud Site | 1 | 0 | 3 | 19 | 28 | 35 | 24 | 27 | 24 | – | 32 | 49 | 4 | 15 | 31 | 1 | 1 | 2 | – | – | – | – | – | – |
Cell Periphery | 22 | 27 | 55 | 45 | 14 | 25 | 27 | 19 | 26 | – | 21 | 26 | 32 | 22 | 24 | 2 | 5 | 4 | 11 | 15 | 53 | 10 | 6 | 5 |
Cytoplasm | 77 | 128 | 262 | 136 | 123 | 120 | 140 | 137 | 83 | – | 78 | 72 | 166 | 118 | 116 | 9 | 16 | 32 | 44 | 91 | 132 | 23 | 17 | 7 |
Endoplasmic Reticulum | 3 | 2 | 6 | 3 | 7 | 8 | 11 | 9 | 3 | – | 3 | 5 | 2 | 3 | 2 | 2 | 2 | 4 | 2 | 2 | 7 | 0 | 2 | 2 |
Endosome | 1 | 1 | 0 | 2 | 5 | 2 | 2 | 4 | 0 | – | 0 | 0 | 5 | 4 | 6 | 0 | 1 | 4 | 0 | 1 | 7 | 1 | 3 | 2 |
Golgi | 1 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 2 | 0 | 0 | 0 |
Mitochondria | 0 | 4 | 6 | 23 | 17 | 58 | 80 | 110 | 101 | – | 159 | 164 | 1 | 1 | 5 | 1 | 1 | 3 | 1 | 5 | 16 | 6 | 5 | 7 |
Nucleus | 0 | 0 | 0 | 1 | 5 | 3 | 13 | 7 | 6 | – | 8 | 12 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | – | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 3 | 8 | 6 | 9 | 12 | – | 8 | 8 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 1 | 4 | 9 | 20 | 26 | 25 | 10 | – | 20 | 35 | 0 | 7 | 17 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 2 | 0 | 12 | 14 | 38 | 24 | 54 | 53 | 21 | – | 32 | 45 | 13 | 14 | 16 | 2 | 10 | 8 | 0 | 0 | 3 | 0 | 1 | 1 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 2 | 1 | – | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 3 | 1 | 2 | 1 |
Unique Cell Count | 97 | 147 | 324 | 222 | 277 | 302 | 358 | 367 | 257 | 329 | 352 | 227 | 224 | 246 | 16 | 39 | 58 | 108 | 167 | 342 | 186 | 290 | 288 | |
Labelled Cell Count | 130 | 181 | 424 | 318 | 375 | 425 | 501 | 526 | 366 | 479 | 529 | 291 | 313 | 348 | 23 | 55 | 76 | 108 | 167 | 342 | 186 | 290 | 288 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.0 | 5.9 | 5.0 | 4.9 | 5.2 | 4.8 | 4.7 | 4.6 | 4.2 | – | 4.0 | 3.8 | 5.6 | 5.7 | 5.0 | 7.4 | 8.1 | 8.3 | 5.9 | 5.8 | 6.4 |
Std Deviation (1e-4) | 0.7 | 1.0 | 1.1 | 1.5 | 1.9 | 1.3 | 1.4 | 1.5 | 1.7 | – | 1.3 | 1.1 | 0.9 | 0.7 | 0.9 | 0.9 | 1.9 | 2.3 | 1.6 | 1.3 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.02 | 0.06 | -0.05 | -0.08 | -0.11 | -0.26 | – | -0.31 | -0.38 | 0.17 | 0.19 | 0.0 | 0.58 | 0.7 | 0.74 | 0.25 | 0.22 | 0.36 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.5 | 1.3 | 0 | 0 | 0 | 0 | – | 0 | 0 | 2.2 | 7.1 | 5.6 | 0 | 0 | 0.6 |
Bud | 1.7 | 2.9 | 0 | 0 | 0 | 0 | – | 0 | 0 | -0.5 | 2.4 | 2.4 | 0 | 0 | -1.0 |
Bud Neck | 1.1 | 3.9 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0.6 | 0.9 | 2.1 | 0 | 0 | 0 |
Bud Site | 4.5 | 5.1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 3.7 | 5.8 | 0 | 0 | 0 |
Cell Periphery | 1.0 | -4.6 | 0 | 0 | 0 | 0 | – | 0 | 0 | -0.9 | -2.4 | -2.5 | 0 | -0.7 | -2.0 |
Cytoplasm | -5.1 | -9.3 | -10.5 | -11.1 | -11.6 | -11.8 | – | -14.6 | -15.7 | -2.1 | -7.0 | -8.4 | -2.4 | -5.6 | -4.3 |
Endoplasmic Reticulum | 0 | 0.6 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.4 | 2.7 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 3.5 | 0 | 0 | – | 0 | 3.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 4.5 | 0 | 0 | 0 |
SpindlePole | 1.4 | 4.4 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1.1 | 1.4 | 1.5 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.1092 | 3.2823 | 3.4387 | 3.1495 | 3.2644 | 3.3444 | 4.2301 | 4.7654 | 4.7304 | 5.0942 | 4.3769 | 4.508 | 4.3181 | 5.2113 | 4.688 | 4.7056 | 4.0988 | 4.788 |
Actin | 0.0605 | 0.0059 | 0.0352 | 0.0104 | 0.0221 | 0.0267 | 0.019 | 0.0091 | 0.04 | 0.0065 | 0.0486 | 0.0096 | 0.0253 | 0.0131 | 0.0307 | 0.0037 | 0.0163 | 0.0107 |
Bud | 0.0039 | 0.0025 | 0.0025 | 0.0018 | 0.0028 | 0.0033 | 0.008 | 0.0094 | 0.0073 | 0.0117 | 0.007 | 0.0062 | 0.0014 | 0.0031 | 0.0027 | 0.0007 | 0.0008 | 0.0009 |
Bud Neck | 0.0277 | 0.014 | 0.4354 | 0.4908 | 0.3005 | 0.124 | 0.0199 | 0.0072 | 0.3595 | 0.5121 | 0.2117 | 0.0912 | 0.04 | 0.002 | 0.3684 | 0.6535 | 0.2655 | 0.1308 |
Bud Periphery | 0.0076 | 0.0032 | 0.0031 | 0.0044 | 0.0067 | 0.0107 | 0.0187 | 0.0078 | 0.0096 | 0.0285 | 0.0147 | 0.0158 | 0.004 | 0.0051 | 0.0068 | 0.001 | 0.0013 | 0.0037 |
Bud Site | 0.1575 | 0.183 | 0.1314 | 0.0145 | 0.0097 | 0.0143 | 0.1509 | 0.1746 | 0.1856 | 0.0322 | 0.0286 | 0.0074 | 0.1671 | 0.1156 | 0.1516 | 0.0484 | 0.0194 | 0.0033 |
Cell Periphery | 0.0306 | 0.0159 | 0.0065 | 0.0057 | 0.0044 | 0.0098 | 0.0229 | 0.0138 | 0.013 | 0.0071 | 0.006 | 0.0072 | 0.0515 | 0.0131 | 0.0139 | 0.0021 | 0.0037 | 0.0092 |
Cytoplasm | 0.399 | 0.6348 | 0.2591 | 0.3526 | 0.5092 | 0.6696 | 0.5083 | 0.6476 | 0.2565 | 0.231 | 0.4869 | 0.685 | 0.5836 | 0.7243 | 0.3381 | 0.2739 | 0.6236 | 0.7171 |
Cytoplasmic Foci | 0.0207 | 0.0097 | 0.0279 | 0.0057 | 0.0073 | 0.0145 | 0.02 | 0.007 | 0.0244 | 0.0339 | 0.0134 | 0.0124 | 0.0103 | 0.0019 | 0.0115 | 0.0055 | 0.0251 | 0.0048 |
Eisosomes | 0.0017 | 0.0002 | 0.0005 | 0.0001 | 0.0003 | 0.0006 | 0.0004 | 0.0003 | 0.0005 | 0.0004 | 0.0003 | 0.0001 | 0.0008 | 0.0003 | 0.0008 | 0.0001 | 0.0003 | 0.0003 |
Endoplasmic Reticulum | 0.0097 | 0.0041 | 0.003 | 0.005 | 0.0035 | 0.0026 | 0.0073 | 0.002 | 0.0016 | 0.0014 | 0.0023 | 0.0024 | 0.0067 | 0.0072 | 0.0018 | 0.0018 | 0.0022 | 0.0033 |
Endosome | 0.044 | 0.0039 | 0.0079 | 0.0353 | 0.0083 | 0.0082 | 0.0146 | 0.0013 | 0.0115 | 0.0464 | 0.0243 | 0.0048 | 0.0152 | 0.0005 | 0.0073 | 0.0014 | 0.0034 | 0.0007 |
Golgi | 0.0179 | 0.0022 | 0.0047 | 0.0044 | 0.0073 | 0.0037 | 0.0025 | 0.0008 | 0.006 | 0.0061 | 0.0156 | 0.0051 | 0.0022 | 0.0004 | 0.0013 | 0.001 | 0.0028 | 0.0004 |
Lipid Particles | 0.0356 | 0.0008 | 0.0047 | 0.012 | 0.0094 | 0.0043 | 0.0102 | 0.0007 | 0.005 | 0.0127 | 0.0158 | 0.0031 | 0.0046 | 0.0004 | 0.0031 | 0.0001 | 0.0014 | 0.0007 |
Mitochondria | 0.0306 | 0.0002 | 0.0025 | 0.0112 | 0.0479 | 0.0016 | 0.0026 | 0.0004 | 0.0032 | 0.0033 | 0.0105 | 0.0046 | 0.0014 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0.0001 |
None | 0.0741 | 0.1163 | 0.0329 | 0.0267 | 0.0395 | 0.0793 | 0.1488 | 0.1097 | 0.0574 | 0.0438 | 0.0713 | 0.1299 | 0.0742 | 0.1108 | 0.05 | 0.0048 | 0.028 | 0.1118 |
Nuclear Periphery | 0.024 | 0.0003 | 0.0079 | 0.0015 | 0.0004 | 0.0036 | 0.0132 | 0.0006 | 0.0007 | 0.0009 | 0.0011 | 0.0014 | 0.0014 | 0.0004 | 0.0012 | 0.0001 | 0.0003 | 0.0002 |
Nucleolus | 0.0064 | 0.0002 | 0.0008 | 0.0007 | 0.0003 | 0.0023 | 0.0015 | 0.0006 | 0.0007 | 0.0027 | 0.0025 | 0.0003 | 0.0002 | 0.0001 | 0.0011 | 0.0001 | 0.0006 | 0.0001 |
Nucleus | 0.0114 | 0.0009 | 0.0058 | 0.0024 | 0.0009 | 0.0026 | 0.0127 | 0.0038 | 0.0032 | 0.0015 | 0.0023 | 0.002 | 0.0009 | 0.0006 | 0.0018 | 0.0006 | 0.0011 | 0.0005 |
Peroxisomes | 0.0178 | 0.0002 | 0.0221 | 0.0017 | 0.013 | 0.0089 | 0.0032 | 0.0003 | 0.0097 | 0.0035 | 0.0178 | 0.0078 | 0.0032 | 0.0001 | 0.0018 | 0.0001 | 0.0009 | 0.0002 |
Punctate Nuclear | 0.0072 | 0.0003 | 0.0032 | 0.0005 | 0.0003 | 0.0059 | 0.0034 | 0.0012 | 0.0024 | 0.002 | 0.011 | 0.0009 | 0.0017 | 0.0002 | 0.0021 | 0.0002 | 0.0023 | 0.0003 |
Vacuole | 0.0086 | 0.0014 | 0.0023 | 0.0101 | 0.0055 | 0.0028 | 0.0103 | 0.0019 | 0.0019 | 0.0102 | 0.0068 | 0.0026 | 0.004 | 0.0007 | 0.0033 | 0.0007 | 0.001 | 0.0011 |
Vacuole Periphery | 0.0033 | 0.0001 | 0.0007 | 0.0027 | 0.0007 | 0.0006 | 0.0017 | 0.0001 | 0.0002 | 0.002 | 0.0016 | 0.0004 | 0.0005 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.644 | 10.1167 | 5.5199 | 12.7689 | 15.7518 | 9.6474 | 12.0341 | 9.1375 | 10.7583 | 20.1735 |
Translational Efficiency | 1.0099 | 0.7517 | 0.8923 | 0.5451 | 0.6568 | 1.3355 | 0.6196 | 0.4871 | 0.5853 | 0.5434 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
616 | 223 | 155 | 1468 | 1761 | 2275 | 171 | 180 | 2377 | 2498 | 326 | 1648 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 707.31 | 828.98 | 1143.94 | 885.87 | 730.79 | 806.89 | 1019.25 | 919.28 | 724.71 | 808.86 | 1078.54 | 889.52 |
Standard Deviation | 76.59 | 103.77 | 119.47 | 113.73 | 96.45 | 104.71 | 102.38 | 109.75 | 92.30 | 104.82 | 127.13 | 113.78 |
Intensity Change Log 2 | — | 0.228995 | 0.693597 | 0.324752 | — | 0.142915 | 0.479979 | 0.331047 | — | 0.185894 | 0.588996 | 0.327955 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002200 | 0.003938 | 0.007474 | 0.008307 | 0.004299 | 0.005365 | 0.002536 | 0.025412 | 0.003755 | 0.005237 | 0.004884 | 0.010175 |
Bud Neck | 0.218454* | 0.103035 | 0.044672 | 0.103957 | 0.180811* | 0.197681* | 0.112519 | 0.104997 | 0.190566* | 0.189232* | 0.080260 | 0.104071 |
Bud Site | 0.077590 | 0.119996* | 0.203289* | 0.246170* | 0.122554* | 0.149211* | 0.170487* | 0.361500* | 0.110901* | 0.146603* | 0.186083* | 0.258767* |
Cell Periphery | 0.007060 | 0.001442 | 0.001100 | 0.000582 | 0.003617 | 0.000956 | 0.001023 | 0.000795 | 0.004510 | 0.000999 | 0.001060 | 0.000605 |
Cytoplasm | 0.478929* | 0.366436* | 0.219905* | 0.330318* | 0.414476* | 0.395621* | 0.202803* | 0.182227* | 0.431179* | 0.393016* | 0.210934* | 0.314143* |
Cytoplasmic Foci | 0.078275 | 0.197585* | 0.054046 | 0.029202 | 0.128622 | 0.103564 | 0.057824 | 0.033944 | 0.115574 | 0.111957 | 0.056028 | 0.029720 |
Eisosomes | 0.009841 | 0.002926 | 0.000269 | 0.000186 | 0.005196 | 0.001752 | 0.000302 | 0.000350 | 0.006400 | 0.001857 | 0.000286 | 0.000204 |
Endoplasmic Reticulum | 0.003409 | 0.000824 | 0.010793 | 0.000598 | 0.001823 | 0.000895 | 0.002630 | 0.001206 | 0.002234 | 0.000889 | 0.006511 | 0.000664 |
Endosome | 0.012179 | 0.044565 | 0.014275 | 0.013335 | 0.017946 | 0.018738 | 0.015699 | 0.007019 | 0.016452 | 0.021044 | 0.015022 | 0.012645 |
Golgi | 0.007882 | 0.010775 | 0.000538 | 0.003731 | 0.010358 | 0.010581 | 0.000406 | 0.002917 | 0.009716 | 0.010599 | 0.000469 | 0.003642 |
Lipid Particles | 0.008407 | 0.006891 | 0.006096 | 0.001049 | 0.006718 | 0.003210 | 0.003845 | 0.001020 | 0.007156 | 0.003539 | 0.004915 | 0.001046 |
Mitochondria | 0.002633 | 0.004775 | 0.000134 | 0.005400 | 0.005834 | 0.007063 | 0.000124 | 0.001329 | 0.005004 | 0.006858 | 0.000129 | 0.004955 |
Mitotic Spindle | 0.000390 | 0.000334 | 0.000734 | 0.035951 | 0.000337 | 0.004102 | 0.002541 | 0.009879 | 0.000350 | 0.003765 | 0.001682 | 0.033103 |
None | 0.001333 | 0.000094 | 0.000325 | 0.000672 | 0.001545 | 0.000616 | 0.000585 | 0.000155 | 0.001490 | 0.000569 | 0.000462 | 0.000615 |
Nuclear Periphery | 0.000194 | 0.000063 | 0.000793 | 0.000228 | 0.000080 | 0.000084 | 0.000455 | 0.000135 | 0.000109 | 0.000082 | 0.000616 | 0.000218 |
Nuclear Periphery Foci | 0.000410 | 0.000755 | 0.014316 | 0.000540 | 0.000309 | 0.000377 | 0.005465 | 0.001001 | 0.000335 | 0.000410 | 0.009674 | 0.000590 |
Nucleolus | 0.000258 | 0.000209 | 0.000227 | 0.000311 | 0.000587 | 0.000284 | 0.000263 | 0.000088 | 0.000502 | 0.000278 | 0.000246 | 0.000287 |
Nucleus | 0.033020 | 0.011315 | 0.084545 | 0.022547 | 0.036465 | 0.020795 | 0.059876 | 0.010368 | 0.035572 | 0.019948 | 0.071605 | 0.021217 |
Peroxisomes | 0.004854 | 0.018691 | 0.010742 | 0.021929 | 0.008828 | 0.012219 | 0.014022 | 0.022549 | 0.007798 | 0.012797 | 0.012462 | 0.021997 |
Vacuole | 0.051643 | 0.103259 | 0.325445* | 0.171766 | 0.048488 | 0.065625 | 0.346374* | 0.228150* | 0.049306 | 0.068985 | 0.336423* | 0.177925 |
Vacuole Periphery | 0.001041 | 0.002092 | 0.000281 | 0.003221 | 0.001108 | 0.001260 | 0.000219 | 0.004959 | 0.001090 | 0.001334 | 0.000248 | 0.003410 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.37 | -1.90 | -3.59 | -1.61 | 0.19 | -1.48 | 2.71 | -2.55 | -2.36 | -2.90 | -2.32 | -0.42 | -3.22 | -1.71 | -2.02 |
Bud Neck | 6.81 | 14.11 | 12.02 | 2.69 | -5.13 | -1.96 | 6.66 | 9.42 | 11.22 | 0.35 | 0.21 | 13.78 | 18.13 | 18.74 | -1.44 |
Bud Site | -3.39 | -2.65 | -10.05 | -3.54 | -1.39 | -4.44 | 0.06 | -5.82 | -4.68 | -4.75 | -6.76 | -1.18 | -8.60 | -3.64 | -3.16 |
Cell Periphery | 8.96 | 11.05 | 12.26 | 3.06 | 5.86 | 6.72 | 6.87 | 7.46 | 2.91 | 1.58 | 10.66 | 10.88 | 12.53 | 7.21 | 6.22 |
Cytoplasm | 5.68 | 12.87 | 9.26 | 0.86 | -7.32 | 2.01 | 11.49 | 11.09 | 10.24 | 0.15 | 4.61 | 16.59 | 10.27 | 6.28 | -8.91 |
Cytoplasmic Foci | -9.58 | 4.57 | 10.73 | 14.25 | 4.62 | 5.24 | 12.68 | 16.13 | 12.66 | 2.32 | 0.87 | 14.57 | 25.45 | 24.49 | 6.02 |
Eisosomes | 3.56 | 10.11 | 10.21 | 1.59 | 4.10 | 7.70 | 11.64 | 11.49 | 9.15 | -0.79 | 10.13 | 15.33 | 15.55 | 8.14 | 5.00 |
Endoplasmic Reticulum | 2.18 | -5.14 | 2.23 | 0.16 | 9.54 | 1.90 | -2.49 | 1.13 | -1.05 | 4.01 | 2.66 | -6.73 | 3.37 | 0.11 | 10.10 |
Endosome | -5.44 | -0.70 | 2.10 | 6.04 | 2.82 | -0.50 | 2.28 | 5.82 | 6.66 | 3.55 | -3.25 | 2.17 | 5.81 | 8.72 | 3.80 |
Golgi | -1.51 | 12.73 | 7.02 | 4.66 | -3.07 | -0.25 | 18.23 | 5.21 | 5.25 | -1.05 | -1.17 | 21.44 | 11.03 | 10.59 | -3.34 |
Lipid Particles | 0.71 | 2.18 | 8.85 | 2.71 | 8.81 | 5.00 | 3.77 | 7.10 | 3.01 | 4.18 | 5.86 | 3.45 | 11.17 | 5.29 | 9.78 |
Mitochondria | -0.81 | 6.66 | -0.68 | 0.52 | -3.53 | -1.07 | 7.47 | 5.29 | 6.15 | -1.42 | -1.86 | 8.48 | 2.00 | 3.37 | -3.64 |
Mitotic Spindle | 0.19 | -1.13 | -8.72 | -8.78 | -8.63 | -4.89 | -1.01 | -2.38 | -1.62 | -1.46 | -4.85 | -1.12 | -9.06 | -7.59 | -7.11 |
None | 3.97 | 3.26 | 1.49 | -7.45 | -4.89 | 3.37 | 2.92 | 4.96 | 2.70 | 1.04 | 4.03 | 4.45 | 3.21 | -1.53 | -2.29 |
Nuclear Periphery | 1.41 | -6.63 | -2.56 | -9.78 | 6.28 | -0.09 | -8.26 | -5.28 | -3.32 | 5.11 | 0.62 | -11.91 | -7.58 | -6.98 | 5.99 |
Nuclear Periphery Foci | -1.92 | -9.63 | -2.53 | 0.74 | 9.47 | -0.38 | -7.92 | -3.42 | -3.04 | 5.33 | -0.50 | -11.67 | -4.61 | -1.99 | 11.15 |
Nucleolus | 0.94 | 1.09 | -0.11 | -0.62 | -0.56 | 1.89 | 2.32 | 3.05 | 2.58 | 2.97 | 1.82 | 2.43 | 1.91 | 0.31 | -0.33 |
Nucleus | 8.79 | -6.75 | -2.61 | -12.89 | 5.94 | 8.82 | -3.51 | 9.83 | 3.28 | 6.03 | 10.24 | -7.03 | -1.01 | -9.02 | 6.56 |
Peroxisomes | -5.62 | -1.54 | -5.30 | 0.99 | -2.74 | -3.28 | -0.54 | -2.22 | -1.28 | -1.14 | -5.57 | -0.76 | -4.24 | -1.52 | -2.19 |
Vacuole | -6.18 | -16.63 | -28.74 | -15.62 | 5.91 | -6.34 | -15.69 | -12.09 | -11.39 | 2.27 | -7.95 | -22.77 | -32.47 | -29.26 | 7.55 |
Vacuole Periphery | -1.30 | 3.22 | -1.34 | 0.21 | -2.83 | -0.85 | 7.96 | -0.67 | -0.62 | -0.96 | -1.36 | 8.19 | -1.49 | -1.08 | -2.94 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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