Standard name
Human Ortholog
Description Basic leucine zipper (bZIP) iron-sensing transcription factor; involved in diauxic shift; YAP5 has a paralog, YAP7, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.96 0.86 0.93 0.85 0.92 0.95 0.9 0.89 0.54 0.65 0.59 0.53 0.72 0.94 0.99 – – – 0.68 0.79 0.61 0.79 0.83 0.81
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0.17 0 0.07 0 0.05 0.08 0.1 0.54 0.44 0.5 0.5 0 0 0 – – – 0.1 0.06 0.13 0.05 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0.16 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0 0.07 0.1 0.05 0 0 0 0 0 0 0.05 0.13 0.05 0 – – – 0.12 0 0.09 0.08 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 – – – 1 2 2 1 1 4
Bud 0 0 0 1 0 0 0 3 3 3 6 7 0 0 0 – – – 0 0 0 0 1 4
Bud Neck 1 1 4 3 2 5 7 11 2 3 1 4 0 1 0 – – – 0 0 0 0 2 1
Bud Site 0 0 0 0 0 1 0 0 0 1 2 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 1 0 0 2 2 0 0 1 0 0 1 0 1 – – – 0 1 0 0 0 0
Cytoplasm 92 135 153 127 161 245 271 368 94 164 141 126 97 284 399 – – – 89 137 113 168 216 268
Endoplasmic Reticulum 0 3 1 0 0 0 0 0 1 0 0 0 3 1 1 – – – 0 0 1 0 2 1
Endosome 0 0 0 2 1 0 0 0 0 1 0 1 1 1 0 – – – 2 1 2 1 1 4
Golgi 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 2 0 0 0
Mitochondria 2 27 0 11 5 13 24 40 93 111 118 119 1 2 1 – – – 13 10 24 9 7 13
Nucleus 0 0 0 2 1 3 8 5 4 7 5 7 22 7 1 – – – 2 2 2 4 2 1
Nuclear Periphery 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 1 0 1 1
Nucleolus 0 0 0 0 0 0 0 0 2 0 1 0 0 0 1 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 1 0 0 0
SpindlePole 0 0 0 0 1 2 1 7 3 9 5 4 0 2 1 – – – 2 3 5 3 4 7
Vac/Vac Membrane 9 1 11 15 8 8 8 10 5 10 7 13 18 14 6 – – – 16 6 15 17 9 10
Unique Cell Count 96 157 165 149 175 258 301 415 173 252 238 237 134 301 403 133 174 184 214 260 333
Labelled Cell Count 104 167 173 163 179 279 321 445 207 310 286 281 143 312 411 133 174 184 214 260 333


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 3.0 5.0 4.8 5.4 4.3 4.0 4.3 3.4 3.7 3.5 3.5 4.6 4.8 4.7 4.0 4.4 4.5 5.1 5.6 5.3
Std Deviation (1e-4) 0.3 0.5 1.4 1.4 2.4 1.6 1.2 1.2 1.3 1.3 0.9 0.7 0.7 1.0 1.1 1.6 1.0 1.2 1.2 1.8 1.6
Intensity Change (Log2) – – – -0.06 0.11 -0.24 -0.32 -0.23 -0.57 -0.45 -0.51 -0.53 -0.14 -0.05 -0.11 -0.33 -0.2 -0.16 0.02 0.15 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.806 -0.3655 -0.7313 -0.9915 -0.5533 -0.7207 2.2766 2.2606 2.1556 2.5652 1.8365 2.2733 -1.4007 -0.8121 -0.9491 -0.6249 -1.0972 -0.9236
Actin 0.0014 0.0007 0.0005 0.0002 0.024 0.0035 0.0339 0.0003 0.0024 0.0007 0.0137 0.0031 0.024 0.0003 0.0064 0.0083 0.0275 0.0008
Bud 0.0004 0.0035 0.0022 0 0.0002 0.0001 0.0002 0.0001 0.0002 0.0001 0.0002 0.0002 0.0008 0.0003 0.0006 0.0006 0.0002 0.0004
Bud Neck 0.0009 0.0005 0.0009 0.0006 0.0029 0.0049 0.0019 0.0002 0.0003 0.001 0.0007 0.0017 0.0021 0.0002 0.0014 0.0012 0.001 0.0043
Bud Periphery 0.0005 0.0006 0.0003 0 0.0002 0.0006 0.0008 0 0.0004 0.0001 0.0004 0.0002 0.0013 0.0002 0.0011 0.0019 0.0004 0.0002
Bud Site 0.0055 0.0037 0.0033 0.0001 0.0004 0.0001 0.0017 0.0006 0.0006 0.0005 0.0005 0.0001 0.0132 0.0031 0.0027 0.0007 0.0005 0.0002
Cell Periphery 0.0001 0.0001 0.0001 0 0.0001 0 0.0044 0 0.0001 0 0 0 0.0002 0.0001 0.0003 0.0002 0.0001 0
Cytoplasm 0.1746 0.1175 0.1493 0.2378 0.0934 0.1509 0.1339 0.2566 0.1732 0.2018 0.1494 0.2614 0.2754 0.2842 0.237 0.2692 0.3978 0.3698
Cytoplasmic Foci 0.0149 0.0162 0.0073 0.0074 0.0092 0.0142 0.0197 0.0077 0.0085 0.004 0.0092 0.0121 0.017 0.0097 0.0061 0.0027 0.0085 0.0139
Eisosomes 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0013 0.0001 0.0001 0.0001 0.0002 0.0001 0.0002 0 0.0002 0.0002 0.0002 0
Endoplasmic Reticulum 0.0042 0.0025 0.0035 0.0012 0.0017 0.002 0.0074 0.0024 0.0025 0.0046 0.0015 0.0022 0.0055 0.0077 0.0051 0.0044 0.0044 0.0044
Endosome 0.0062 0.0029 0.0034 0.0025 0.0042 0.0032 0.0083 0.0025 0.002 0.0026 0.004 0.0042 0.0104 0.0138 0.0094 0.0189 0.0155 0.0049
Golgi 0.0005 0.0002 0.0001 0.0001 0.0024 0.0002 0.0034 0.0001 0.0002 0.0001 0.0021 0.0005 0.0044 0.0022 0.0032 0.0061 0.0234 0.0004
Lipid Particles 0.0036 0.0008 0.0005 0.0002 0.0021 0.001 0.008 0.0002 0.0005 0.0003 0.0118 0.0018 0.0076 0.0005 0.0059 0.0021 0.0416 0.0021
Mitochondria 0.0009 0.001 0.0007 0.0008 0.0007 0.0007 0.0017 0.0006 0.0007 0.0008 0.0027 0.0007 0.0053 0.001 0.0062 0.0083 0.0062 0.0006
None 0.5322 0.7312 0.6036 0.5866 0.7274 0.5191 0.6827 0.6279 0.7109 0.6071 0.6685 0.5288 0.4658 0.5704 0.5665 0.4016 0.3963 0.3476
Nuclear Periphery 0.0153 0.0066 0.0195 0.0076 0.007 0.0118 0.0134 0.0053 0.0049 0.0129 0.0095 0.0092 0.0146 0.0078 0.0107 0.0117 0.0063 0.0084
Nucleolus 0.0034 0.0027 0.0034 0.0013 0.002 0.0047 0.001 0.001 0.0012 0.0013 0.0019 0.0014 0.0019 0.001 0.0007 0.0013 0.0003 0.002
Nucleus 0.1963 0.0566 0.1549 0.1068 0.0919 0.1972 0.0494 0.075 0.0692 0.1445 0.0846 0.1281 0.1185 0.0838 0.1233 0.248 0.0566 0.1993
Peroxisomes 0.0018 0.0012 0.0006 0.0002 0.0045 0.0004 0.0124 0.0002 0.0028 0.0003 0.0154 0.002 0.0067 0.0001 0.0008 0.0002 0.0036 0.0036
Punctate Nuclear 0.0349 0.0502 0.0437 0.0456 0.0247 0.0843 0.0123 0.0176 0.0179 0.0161 0.0224 0.041 0.0189 0.01 0.0079 0.004 0.0073 0.0346
Vacuole 0.0018 0.0011 0.0015 0.0006 0.0006 0.0007 0.0017 0.0012 0.0012 0.001 0.0008 0.0008 0.005 0.0031 0.0032 0.005 0.0014 0.0021
Vacuole Periphery 0.0006 0.0002 0.0004 0.0002 0.0003 0.0002 0.0006 0.0002 0.0002 0.0003 0.0004 0.0003 0.0012 0.0005 0.0012 0.0033 0.0009 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.7086 9.3559 18.8438 18.8725 19.385 32.8746 25.0007 37.3959 33.3274 24.8004
Translational Efficiency 1.5857 2.3147 1.0666 0.9319 0.7005 0.9133 0.9594 0.7882 0.8741 0.8912

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
621 960 2836 1772 1841 1647 402 1576 2462 2607 3238 3348

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 577.59 697.25 767.19 795.24 680.53 756.42 823.88 742.82 654.57 734.63 774.23 770.56
Standard Deviation 82.60 89.90 90.53 110.32 75.85 90.30 90.24 126.99 89.56 94.56 92.40 121.31
Intensity Change Log 2 — 0.271630 0.409538 0.461345 — 0.152529 0.275775 0.126354 — 0.208430 0.338728 0.289842

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000069 0.000269 0.000119 0.000584 0.000040 0.000350 0.000131 0.000235 0.000047 0.000320 0.000120 0.000420
Bud Neck 0.008629 0.014609 0.008710 0.011182 0.002111 0.019129 0.001809 0.009382 0.003755 0.017465 0.007854 0.010335
Bud Site 0.001052 0.006944 0.008781 0.025647 0.000722 0.009105 0.001069 0.028874 0.000805 0.008310 0.007824 0.027166
Cell Periphery 0.000190 0.000139 0.000139 0.000198 0.000093 0.000330 0.000169 0.000870 0.000118 0.000260 0.000143 0.000514
Cytoplasm 0.340043* 0.380420* 0.277408* 0.276970* 0.447471* 0.368575* 0.207509* 0.334346* 0.420374* 0.372937* 0.268730* 0.303979*
Cytoplasmic Foci 0.168138 0.142453 0.003706 0.006735 0.070305 0.145748 0.014476 0.016095 0.094982 0.144535 0.005044 0.011141
Eisosomes 0.000146 0.000082 0.000017 0.000034 0.000039 0.000111 0.000070 0.000083 0.000066 0.000101 0.000024 0.000057
Endoplasmic Reticulum 0.000675 0.001677 0.001817 0.004395 0.000702 0.000946 0.002263 0.001183 0.000695 0.001215 0.001872 0.002883
Endosome 0.004816 0.007561 0.001154 0.005267 0.001434 0.006905 0.001840 0.001957 0.002287 0.007146 0.001239 0.003709
Golgi 0.000958 0.002092 0.000097 0.002388 0.000234 0.002435 0.000054 0.000377 0.000417 0.002308 0.000091 0.001441
Lipid Particles 0.011799 0.007221 0.000533 0.001063 0.002852 0.007518 0.002325 0.001277 0.005109 0.007408 0.000756 0.001164
Mitochondria 0.005730 0.010957 0.001798 0.004074 0.001248 0.012306 0.000306 0.001061 0.002378 0.011809 0.001613 0.002656
Mitotic Spindle 0.000818 0.003675 0.008836 0.026564 0.000380 0.005901 0.000279 0.006689 0.000490 0.005082 0.007773 0.017208
None 0.021185 0.007839 0.005063 0.006549 0.007912 0.005708 0.013279 0.030716 0.011260 0.006493 0.006083 0.017925
Nuclear Periphery 0.001021 0.001073 0.003115 0.003024 0.000656 0.000875 0.001513 0.001090 0.000748 0.000948 0.002916 0.002114
Nuclear Periphery Foci 0.000325 0.000811 0.000246 0.001286 0.000311 0.000442 0.000573 0.000451 0.000315 0.000578 0.000286 0.000893
Nucleolus 0.004100 0.003905 0.000759 0.000602 0.001110 0.002530 0.001235 0.001587 0.001864 0.003036 0.000818 0.001066
Nucleus 0.408573* 0.364521* 0.660369* 0.574772* 0.453484* 0.374500* 0.713786* 0.535716* 0.442156* 0.370826* 0.667001* 0.556387*
Peroxisomes 0.007341 0.009406 0.000402 0.000722 0.000923 0.011041 0.000287 0.000400 0.002542 0.010439 0.000388 0.000570
Vacuole 0.013862 0.032796 0.016411 0.046686 0.007746 0.024107 0.036910 0.025973 0.009289 0.027307 0.018956 0.036936
Vacuole Periphery 0.000530 0.001551 0.000519 0.001256 0.000228 0.001437 0.000116 0.001635 0.000304 0.001479 0.000469 0.001434

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.15 -3.04 -5.71 -2.94 -5.04 -2.42 -6.62 -6.66 0.73 -0.36 -3.36 -5.98 -7.73 -0.89 -5.69
Bud Neck -2.96 -0.47 -1.55 1.99 -1.61 -10.66 -2.01 -6.92 5.32 -4.78 -11.44 -6.88 -8.48 5.47 -1.72
Bud Site -4.35 -6.91 -7.51 -3.75 -3.47 -6.28 -3.39 -8.90 -5.24 -8.23 -7.67 -7.45 -11.70 -6.18 -6.78
Cell Periphery 3.45 4.43 2.32 -0.20 -0.96 -1.96 -6.55 -5.43 -2.35 -4.70 -1.86 -1.69 -5.38 -1.66 -5.07
Cytoplasm -2.97 8.22 9.77 14.35 2.82 9.20 21.46 14.78 5.82 -10.35 6.75 26.43 22.20 15.17 -4.59
Cytoplasmic Foci 2.46 21.40 21.14 23.55 -3.48 -14.51 18.26 19.35 28.60 -0.47 -11.00 30.48 28.60 37.58 -8.06
Eisosomes 6.39 14.68 11.61 9.02 -14.04 -11.01 -7.79 -8.07 4.28 1.38 -7.04 13.11 1.60 8.45 -12.56
Endoplasmic Reticulum -5.56 -10.26 -16.74 -12.85 -10.81 -1.13 -10.23 -4.89 -3.04 7.32 -3.44 -12.74 -16.22 -12.68 -5.82
Endosome -2.38 3.84 1.77 5.58 -3.80 -8.56 -3.77 -1.88 7.65 2.02 -9.07 3.57 -0.39 8.75 -3.99
Golgi -1.96 3.58 -0.03 1.73 -2.28 -4.11 4.16 -0.78 3.96 -3.31 -4.78 4.87 -1.07 3.72 -2.77
Lipid Particles 3.54 10.32 9.69 10.31 -8.70 -7.82 0.46 7.34 10.94 5.02 -4.48 13.22 11.87 14.77 -5.49
Mitochondria -2.37 2.84 2.23 4.81 -1.75 -7.29 2.87 0.71 7.56 -4.13 -7.84 1.67 1.18 8.68 -0.74
Mitotic Spindle -1.52 -4.53 -6.37 -5.45 -3.31 -4.02 -1.31 -5.41 -0.80 -4.82 -4.49 -6.04 -8.54 -4.72 -2.74
None 4.11 5.45 5.21 2.02 -0.85 2.41 -1.05 -6.58 -7.51 -4.56 4.35 5.89 -2.14 -5.93 -7.15
Nuclear Periphery -0.35 -14.48 -15.70 -17.98 -4.28 -2.11 -11.44 -10.15 -5.54 4.83 -2.38 -23.49 -20.62 -18.23 2.53
Nuclear Periphery Foci -5.35 0.46 -7.31 -2.74 -7.68 -1.14 -5.53 -1.84 -0.50 3.91 -3.73 -0.98 -6.94 -3.44 -6.84
Nucleolus 0.50 3.94 4.06 6.16 0.81 -3.69 -0.15 -1.87 2.51 -1.48 -2.80 4.15 3.31 6.22 -1.71
Nucleus 3.06 -22.15 -13.89 -19.75 10.22 8.44 -15.62 -9.93 -17.27 8.63 9.17 -32.23 -17.16 -26.77 15.04
Peroxisomes -1.53 6.93 6.95 8.45 -0.34 -8.84 2.30 2.16 9.80 -0.68 -9.07 6.08 6.45 12.73 -0.31
Vacuole -6.92 -4.25 -19.50 -13.86 -17.94 -11.19 -9.28 -13.75 -5.62 5.07 -13.66 -13.20 -24.57 -15.69 -14.24
Vacuole Periphery -3.75 0.63 -0.24 3.16 -0.71 -4.78 3.46 -2.48 0.18 -2.69 -6.29 -0.68 -2.73 1.81 -2.13
Standard name
Human Ortholog
Description Basic leucine zipper (bZIP) iron-sensing transcription factor; involved in diauxic shift; YAP5 has a paralog, YAP7, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (27%), nucleus (8%), mixed (54%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Yap5

Yap5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yap5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available