ORF
Human Ortholog
Description Putative phosphatidylinositol/phosphatidylcholine transfer protein; possibly involved in lipid metabolism; localizes to the nucleus; contains a CRAL/TRIO domain and binds several lipids in a large-scale study; YKL091C has a paralog, SEC14, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.34 0.36 – 0.5 0.49 0.47 0.33 0.26 0.21 0.22 0.16 0.16 0.83 0.6 0.61 0.28 0.28 0.21 0.38 0.2 0.3 0.69 0.68 0.69
Endoplasmic Reticulum 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.35 0.09 – 0.09 0 0.28 0.4 0.41 0.59 0.49 0.67 0.44 0 0 0 0 0 0 0.05 0 0 0 0 0
Nucleus 0.2 0.29 – 0.33 0.35 0.26 0.33 0.41 0.37 0.47 0.44 0.54 0.08 0.07 0.12 0.36 0.25 0.35 0.31 0.44 0.33 0.11 0.08 0
Nuclear Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.06 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.31 0.52 – 0.18 0.19 0.15 0.17 0.07 0.09 0.07 0.05 0.1 0.12 0.45 0.39 0.48 0.58 0.47 0.16 0.28 0.19 0.07 0.13 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud 1 0 – 1 0 2 1 5 8 10 12 7 0 0 0 0 0 0 2 2 0 0 2 6
Bud Neck 0 0 – 0 2 0 2 3 0 5 0 1 1 0 0 0 0 0 0 0 0 0 2 3
Bud Site 0 0 – 0 0 0 0 4 6 10 2 7 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 1 1 0 1 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0
Cytoplasm 37 21 – 115 151 135 157 116 75 98 59 54 49 79 71 54 50 31 47 18 13 27 96 120
Endoplasmic Reticulum 1 1 – 0 3 0 0 0 0 0 0 0 0 1 0 13 6 5 0 0 1 0 0 1
Endosome 1 0 – 0 2 3 1 1 1 0 0 0 0 3 3 4 12 3 1 0 0 0 0 2
Golgi 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 2 1 2 0 0 0 0 0 1
Mitochondria 38 5 – 21 10 79 188 181 211 221 246 151 0 2 0 6 1 4 5 0 0 0 1 2
Nucleus 22 17 – 76 108 73 159 182 133 213 161 186 5 9 14 71 45 52 39 41 14 4 11 3
Nuclear Periphery 0 0 – 0 0 0 1 1 2 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 6 0 – 0 0 0 1 0 0 0 1 1 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 1 1 0 6 2 3 1 2 0 0 0 0 0 1 4 0 1 0 2 4
Vac/Vac Membrane 33 30 – 41 60 44 79 30 31 32 20 33 7 59 45 94 104 71 19 26 8 2 18 19
Unique Cell Count 108 58 229 309 286 475 440 355 451 365 343 59 131 116 196 180 150 125 95 44 39 141 175
Labelled Cell Count 139 74 255 338 338 589 530 469 592 504 442 62 153 134 244 220 170 125 95 44 39 141 175


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 5.2 – 5.1 5.3 4.5 4.3 4.5 4.1 4.3 3.9 4.4 5.7 7.2 8.1 7.4 8.5 8.4 – 5.9 6.2
Std Deviation (1e-4) 2.0 0.9 – 1.2 1.4 1.6 1.5 1.3 1.6 1.1 1.4 1.3 1.1 3.9 16.7 1.5 2.0 2.0 – 1.7 1.4
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.1564 0.4762 0.7071 0.2924 0.6623 0.3466 0.261 0.9192 0.862 0.7811 0.5759 0.6557 -0.3331 0.3882 0.1212 0.2988 -0.2123 0.2357
Actin 0.0284 0.0005 0.0194 0.0275 0.014 0.0153 0.0192 0.0001 0.0115 0.0347 0.0053 0.0017 0.0077 0.0003 0.0003 0.0006 0.0001 0.0004
Bud 0.0037 0.004 0.0041 0.0038 0.0094 0.0007 0.0005 0.0003 0.0031 0.0005 0.0123 0.0002 0.0002 0.0109 0.0001 0.0001 0.0001 0.0023
Bud Neck 0.0114 0.0005 0.0013 0.0037 0.0018 0.0039 0.0225 0.0004 0.0107 0.0181 0.0082 0.0028 0.0007 0.0006 0.0004 0.0009 0.0013 0.0023
Bud Periphery 0.0045 0.0017 0.0077 0.0026 0.0171 0.002 0.0006 0.0003 0.0023 0.0009 0.0163 0.0002 0.0001 0.0016 0 0 0 0.001
Bud Site 0.0109 0.0066 0.0045 0.0046 0.0069 0.0006 0.004 0.0024 0.0097 0.0012 0.0053 0.0003 0.0018 0.0102 0.0005 0.0004 0.0001 0.0002
Cell Periphery 0.0005 0.0001 0.0003 0.0003 0.0005 0.0001 0.0002 0.0001 0.0003 0.0001 0.0008 0 0.0001 0.0002 0 0 0 0.0001
Cytoplasm 0.2025 0.3895 0.2381 0.2084 0.2287 0.2762 0.2836 0.3431 0.3465 0.3151 0.2975 0.3665 0.4018 0.4323 0.3976 0.2195 0.2495 0.37
Cytoplasmic Foci 0.04 0.0065 0.0179 0.0149 0.0292 0.0111 0.0407 0.0034 0.0132 0.0095 0.0377 0.0108 0.0065 0.0045 0.0043 0.0122 0.0006 0.0057
Eisosomes 0.0003 0 0.0003 0.0004 0.0003 0.0001 0.0002 0 0.0002 0.0001 0.0001 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0126 0.0044 0.0061 0.004 0.0072 0.0038 0.0054 0.0031 0.0041 0.0036 0.0048 0.0026 0.0095 0.0045 0.008 0.0027 0.0012 0.0036
Endosome 0.0509 0.004 0.0231 0.0101 0.0612 0.0079 0.032 0.0017 0.0113 0.0069 0.0404 0.0075 0.006 0.0067 0.0064 0.0033 0.0012 0.0047
Golgi 0.0096 0.0002 0.0069 0.0036 0.0166 0.0022 0.0071 0.0001 0.0054 0.01 0.0108 0.0043 0.0004 0.0002 0.0001 0.0002 0 0.0001
Lipid Particles 0.0216 0.0002 0.0101 0.0083 0.0408 0.0048 0.0365 0.0001 0.0049 0.0187 0.0289 0.0109 0.0005 0.0001 0.0003 0.0001 0 0.0002
Mitochondria 0.0099 0.0008 0.0081 0.0035 0.028 0.0026 0.0134 0.0004 0.004 0.005 0.0037 0.0038 0.0005 0.0007 0.0004 0.0005 0.0003 0.0016
None 0.2417 0.3036 0.2803 0.2139 0.1705 0.2617 0.3509 0.4042 0.3119 0.1349 0.22 0.1844 0.1837 0.1236 0.1596 0.1507 0.0035 0.0421
Nuclear Periphery 0.0549 0.014 0.0214 0.0229 0.0347 0.0146 0.0153 0.0056 0.0101 0.0164 0.0152 0.0072 0.0269 0.0162 0.021 0.0145 0.0134 0.0139
Nucleolus 0.0077 0.0017 0.003 0.0033 0.0056 0.0024 0.0019 0.001 0.0018 0.0012 0.0051 0.0015 0.0045 0.001 0.0009 0.0017 0.0008 0.002
Nucleus 0.2131 0.2387 0.2874 0.3845 0.1899 0.3324 0.1324 0.2259 0.2207 0.3751 0.1959 0.3726 0.3341 0.3724 0.3881 0.5791 0.7127 0.5203
Peroxisomes 0.0221 0.002 0.0143 0.0043 0.0488 0.0074 0.0134 0.0001 0.0072 0.0384 0.022 0.0084 0.0001 0.0001 0.0001 0.0015 0 0.0001
Punctate Nuclear 0.0414 0.0175 0.0404 0.0729 0.0786 0.0474 0.0152 0.0059 0.0171 0.0069 0.0538 0.0117 0.0094 0.0106 0.0087 0.0101 0.0133 0.0264
Vacuole 0.0082 0.0031 0.0037 0.0018 0.0066 0.0021 0.0039 0.0017 0.0033 0.0021 0.0133 0.0023 0.0048 0.0029 0.0026 0.0014 0.0016 0.0023
Vacuole Periphery 0.0042 0.0005 0.0017 0.0006 0.0036 0.0007 0.0011 0.0002 0.0008 0.0007 0.0027 0.0004 0.0006 0.0004 0.0005 0.0003 0.0002 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.2785 6.3433 10.9829 14.2375 9.9668 8.2739 8.8188 10.4612 22.7842 14.9014
Translational Efficiency 1.8484 1.8647 1.2309 1.7255 1.3403 3.4619 2.4533 2.3075 1.3268 1.4707

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
968 496 2110 572 2804 321 155 1012 3772 817 2265 1584

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 689.66 778.88 784.48 912.27 699.05 747.26 1010.85 916.26 696.64 766.46 799.97 914.82
Standard Deviation 81.25 99.77 109.31 137.12 84.80 117.24 132.01 135.33 84.00 108.08 124.86 135.99
Intensity Change Log 2 — 0.175516 0.185851 0.403576 — 0.096215 0.532101 0.390361 — 0.136142 0.370504 0.396939

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000123 0.000472 0.000295 0.000759 0.000121 0.000450 0.000626 0.001995 0.000122 0.000463 0.000318 0.001549
Bud Neck 0.008128 0.008720 0.008707 0.007959 0.009588 0.012049 0.001916 0.012817 0.009214 0.010028 0.008242 0.011062
Bud Site 0.002522 0.006701 0.009608 0.015317 0.005011 0.012574 0.001906 0.039194 0.004372 0.009009 0.009081 0.030572
Cell Periphery 0.000174 0.000170 0.000164 0.000216 0.000194 0.000257 0.000259 0.000201 0.000189 0.000204 0.000170 0.000206
Cytoplasm 0.363509* 0.318394* 0.301349* 0.281344* 0.363384* 0.322262* 0.129264* 0.388268* 0.363416* 0.319914* 0.289573* 0.349656*
Cytoplasmic Foci 0.141433 0.103809 0.004601 0.007573 0.035396 0.134376 0.013951 0.019252 0.062608 0.115819 0.005240 0.015035
Eisosomes 0.000101 0.000077 0.000023 0.000051 0.000033 0.000120 0.000097 0.000046 0.000050 0.000094 0.000028 0.000047
Endoplasmic Reticulum 0.000966 0.003337 0.002909 0.010266 0.000942 0.002367 0.009045 0.003161 0.000948 0.002956 0.003329 0.005727
Endosome 0.004049 0.006550 0.001583 0.004926 0.001703 0.013005 0.002995 0.009548 0.002305 0.009086 0.001679 0.007879
Golgi 0.001183 0.001255 0.000464 0.007537 0.000339 0.003300 0.000095 0.002769 0.000555 0.002058 0.000439 0.004491
Lipid Particles 0.006960 0.004205 0.000639 0.001390 0.002441 0.009904 0.002903 0.003176 0.003600 0.006444 0.000793 0.002531
Mitochondria 0.002972 0.005375 0.001245 0.001085 0.003845 0.006898 0.000393 0.005274 0.003621 0.005974 0.001186 0.003761
Mitotic Spindle 0.001270 0.008654 0.006822 0.015878 0.003293 0.015729 0.000290 0.044526 0.002774 0.011434 0.006375 0.034181
None 0.006302 0.004222 0.005146 0.007194 0.006125 0.005798 0.003529 0.009556 0.006170 0.004841 0.005035 0.008703
Nuclear Periphery 0.000623 0.002006 0.001942 0.003818 0.000992 0.000893 0.001442 0.002843 0.000897 0.001568 0.001908 0.003195
Nuclear Periphery Foci 0.000609 0.001040 0.000687 0.004271 0.000258 0.001309 0.002449 0.002484 0.000348 0.001145 0.000807 0.003130
Nucleolus 0.001395 0.002716 0.000570 0.000450 0.001655 0.002852 0.001206 0.003501 0.001588 0.002770 0.000614 0.002399
Nucleus 0.436302* 0.477525* 0.629920* 0.534512* 0.552523* 0.391367* 0.783994* 0.329305* 0.522698* 0.443674* 0.640463* 0.403407*
Peroxisomes 0.004237 0.007501 0.000249 0.000189 0.001880 0.013967 0.000418 0.006706 0.002485 0.010042 0.000260 0.004353
Vacuole 0.016790 0.036084 0.022419 0.094024 0.009817 0.048437 0.043054 0.107717 0.011607 0.040938 0.023831 0.102772
Vacuole Periphery 0.000352 0.001186 0.000661 0.001242 0.000459 0.002087 0.000168 0.007663 0.000431 0.001540 0.000627 0.005344

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.87 -3.32 -7.48 -2.75 -5.45 -3.83 -6.10 -3.59 -1.91 -0.35 -4.95 -3.86 -5.24 -2.60 -3.75
Bud Neck -0.71 -0.77 0.15 0.94 1.05 -1.11 5.34 -1.48 0.19 -6.32 -0.89 0.36 -0.97 0.09 -1.26
Bud Site -3.10 -4.91 -3.47 -0.86 0.03 -2.21 2.80 -5.57 -1.29 -6.84 -2.94 -3.34 -6.27 -2.43 -3.01
Cell Periphery 0.20 1.42 -0.27 -0.37 -1.49 -1.65 -2.42 2.62 3.34 5.03 -0.74 1.50 2.17 2.67 0.43
Cytoplasm 3.29 8.95 12.88 7.39 6.60 3.37 17.62 11.69 3.34 -9.22 4.93 15.06 17.26 6.90 3.15
Cytoplasmic Foci 4.05 23.59 23.46 13.69 0.05 -9.76 8.87 13.84 12.48 0.86 -8.46 27.75 25.47 18.22 -3.47
Eisosomes 2.73 14.66 6.46 2.48 -11.76 -7.87 -8.52 -12.83 4.78 4.20 -7.86 8.29 -6.57 5.10 -14.94
Endoplasmic Reticulum -5.05 -11.96 -16.15 -9.78 -11.48 -4.36 -8.73 -17.06 -5.99 4.77 -6.64 -15.91 -22.80 -10.22 -9.82
Endosome -2.41 5.04 1.92 3.56 -1.79 -5.47 -2.76 -4.85 4.13 -1.18 -6.71 2.43 -3.13 5.15 -4.69
Golgi -0.08 2.10 -0.54 -0.51 -1.11 -1.77 3.14 -1.91 1.50 -3.25 -2.13 1.06 -1.15 0.89 -1.49
Lipid Particles 3.17 9.71 8.14 4.08 -6.15 -3.35 -0.94 0.82 3.49 1.42 -2.85 10.69 4.58 4.63 -3.42
Mitochondria -0.96 1.59 1.71 2.03 0.76 -1.30 5.71 2.62 2.33 -4.04 -1.32 4.56 3.37 2.77 -1.80
Mitotic Spindle -2.27 -4.47 -4.53 -2.22 -2.97 -2.08 4.08 -9.35 -4.72 -10.08 -2.80 -3.25 -10.26 -6.16 -8.74
None 2.53 2.01 0.44 -2.27 -1.68 0.51 4.48 -1.96 -1.68 -5.53 2.43 3.26 -1.36 -3.41 -4.28
Nuclear Periphery -1.45 -17.81 -6.55 -1.93 -3.16 0.38 -5.15 -13.50 -13.08 -9.03 -1.18 -13.66 -13.42 -4.29 -7.42
Nuclear Periphery Foci -2.38 -0.46 -4.16 -3.35 -4.06 -3.71 -2.91 -5.74 -3.01 -0.80 -5.58 -3.99 -7.20 -4.67 -5.76
Nucleolus -2.15 5.77 5.68 3.97 -0.19 -1.50 2.43 -0.60 1.21 -1.84 -2.31 6.45 0.48 2.37 -2.87
Nucleus -2.09 -18.39 -7.83 -4.50 6.52 8.76 -11.97 12.80 -1.13 17.89 6.65 -17.46 6.23 -1.87 19.65
Peroxisomes -1.90 7.72 7.87 4.50 1.45 -4.14 4.39 0.12 4.12 -2.61 -4.87 7.85 2.80 5.67 -2.83
Vacuole -6.31 -7.47 -17.51 -12.94 -15.42 -7.93 -6.09 -25.49 -14.56 -12.39 -10.86 -12.73 -30.96 -20.68 -25.77
Vacuole Periphery -2.58 -0.86 -0.33 1.86 0.30 -2.28 2.28 -2.11 0.17 -2.58 -3.17 -0.33 -2.09 0.32 -1.80
ORF
Human Ortholog
Description Putative phosphatidylinositol/phosphatidylcholine transfer protein; possibly involved in lipid metabolism; localizes to the nucleus; contains a CRAL/TRIO domain and binds several lipids in a large-scale study; YKL091C has a paralog, SEC14, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (22%), nucleus (11%), mixed (56%)
Cell Cycle Regulation No
Subcompartmental Group N/A

YKL091C

YKL091C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ykl091c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available