Standard name
Human Ortholog
Description Snf2p family member with ATP-dependent chromatin remodeling activity; has a role in silencing at the mating type locus, telomeres and centromeres; enriched at centromeres and is required for correct chromatin structure around centromeres, as well as at the boundary element of the silent HMR; recruited to DNA double-strand breaks (DSBs) where it promotes 5' strand resection of DSBs; potential Cdc28p substrate
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.05 0.07 0.06 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.09 0.08 0.12 0.08 0.1 0.05 0.07 0.09 0.13 0.14 0 0.08 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.08 0.1 0.24 0.07 0.24 0.33 0.38 0.61 0.58 0.58 0.69 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.96 0.91 0.93 0.96 0.95 0.86 0.79 0.8 0.75 0.74 0.79 0.7 0.88 0.84 0.81 0.89 0.84 0.86 0.95 0.91 0.9 0.88 0.69 0.59
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0.05 0 0.06 0.06 0.05 0.06 0.11 0.1 0 0 0 0 0 0 0 0 0 0 0.12 0.11
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0.08 0.13 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0
Bud 0 0 1 0 0 0 2 0 2 5 2 1 0 0 0 1 0 0 0 3 2 0 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 1 0 0 1 3 7 8 8 17 7 9 1 2 4 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 15 5 3 6 14 22 26 13 23 6 13 37 58 68 11 11 14 0 2 0 0 1 0
Endoplasmic Reticulum 0 1 1 0 0 0 0 0 1 0 0 0 0 1 0 3 1 3 0 0 2 0 1 0
Endosome 0 2 0 0 0 0 0 0 0 0 0 0 0 1 4 0 1 1 0 5 1 0 2 4
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2 0 1 4
Mitochondria 0 29 23 36 13 35 86 81 104 139 70 131 1 2 10 1 0 4 0 5 1 0 3 10
Nucleus 72 350 210 147 175 127 207 172 127 176 95 133 353 378 392 242 116 166 82 345 204 150 180 207
Nuclear Periphery 0 3 1 0 0 2 1 0 0 5 2 4 2 3 1 0 1 0 0 0 0 0 0 4
Nucleolus 0 8 1 5 9 4 15 12 9 15 13 18 4 11 14 5 5 6 0 1 0 3 30 39
Peroxisomes 0 1 0 0 0 0 0 0 0 0 0 0 2 1 7 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 1 0 0 7 7 6 0 0 0 0 1 0 0 0 3
Vac/Vac Membrane 0 8 3 1 0 2 4 0 1 0 0 2 0 4 2 11 8 6 1 11 8 12 34 75
Unique Cell Count 75 383 225 153 185 148 262 216 170 239 120 189 399 450 483 271 138 192 87 379 227 171 261 353
Labelled Cell Count 75 418 245 192 206 187 344 299 265 381 195 311 408 468 508 274 144 200 87 379 227 171 261 353


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.8 7.2 5.8 5.4 5.4 5.0 4.6 4.6 4.0 4.0 3.9 3.7 7.6 7.4 6.6 13.0 14.0 13.9 7.6 8.8 8.7
Std Deviation (1e-4) 1.8 1.7 1.2 1.1 1.2 1.5 1.6 1.6 1.6 2.2 1.1 1.4 2.1 2.4 2.3 3.5 3.9 3.2 1.8 1.6 1.7
Intensity Change (Log2) – – – -0.12 -0.09 -0.22 -0.34 -0.34 -0.54 -0.53 -0.59 -0.64 0.39 0.35 0.19 1.16 1.27 1.25 0.38 0.59 0.58


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 3.1 3.0 4.0 2.6 3.3 0 2.3 3.4 4.5 4.8 1.2 2.6 2.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.5 -1.1 3.5 0 0 0 0 0 0 -6.2 -6.3 -4.8 -5.1 -3.9 -3.4
Nucleus 1.1 0.5 -2.4 -4.5 -4.2 -5.2 -5.7 -3.9 -6.2 -2.0 -3.4 -4.2 -1.6 -2.8 -2.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 1.8 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.148 6.1543 5.0903 5.2863 4.4384 5.3225 4.0674 3.7096 3.6237 3.391 2.1374 3.493 8.3208 8.8082 8.3118 7.6802 8.4147 8.2983
Actin 0.0702 0 0.001 0 0 0.0065 0.0189 0 0.0086 0.0102 0.0009 0.0018 0.0211 0 0.0405 0 0 0
Bud 0.0015 0 0.0003 0 0 0.0001 0.0005 0 0.0001 0.0005 0.0004 0.0001 0.0012 0 0.0038 0 0 0
Bud Neck 0.0018 0 0.0006 0 0.0002 0.0011 0.0024 0 0.0005 0.0022 0.0007 0.0005 0.0013 0 0.0007 0 0.0002 0.0002
Bud Periphery 0.0039 0 0.0007 0 0.0001 0.0003 0.0008 0 0.0001 0.0007 0.0006 0.0001 0.0034 0 0.0082 0 0.0001 0
Bud Site 0.005 0 0.0013 0 0.0001 0.0002 0.0012 0 0.0008 0.0041 0.0009 0.0002 0.0032 0 0.0043 0 0.0001 0
Cell Periphery 0.0009 0 0.0003 0 0 0.0001 0.0004 0 0.0001 0.0001 0.0004 0.0001 0.0005 0 0.0002 0 0 0
Cytoplasm 0.0018 0 0.0007 0 0.0001 0.0006 0.0078 0 0.0013 0.0015 0.0052 0.0005 0.0058 0 0.0006 0 0.0001 0
Cytoplasmic Foci 0.0115 0 0.0056 0 0 0.0006 0.0135 0 0.0042 0.0153 0.024 0.0064 0.0372 0 0.0008 0 0 0
Eisosomes 0.0011 0 0.0001 0 0 0.0001 0.0003 0 0.0001 0.0001 0.0004 0 0.0002 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0014 0 0.0004 0 0 0.0007 0.0032 0 0.0006 0.0012 0.0015 0.0002 0.0059 0 0.001 0 0 0
Endosome 0.0063 0 0.005 0 0 0.0012 0.009 0 0.0011 0.012 0.0045 0.0021 0.0311 0 0.0034 0 0.0001 0
Golgi 0.0033 0 0.0017 0 0 0.0011 0.0028 0 0.0005 0.005 0.0006 0.0012 0.0085 0 0.0046 0 0 0
Lipid Particles 0.0072 0 0.0193 0 0 0.0029 0.0067 0 0.0014 0.009 0.0128 0.0032 0.0392 0 0.0004 0 0 0
Mitochondria 0.0189 0.0001 0.0118 0 0.0009 0.0018 0.011 0.0001 0.0007 0.001 0.0415 0.0031 0.027 0.0001 0.001 0.0001 0.0003 0
None 0.0123 0 0.0002 0 0 0.0005 0.0074 0 0.0012 0.0016 0.0197 0.0004 0.0045 0 0.0003 0 0 0
Nuclear Periphery 0.0083 0.0003 0.0017 0.0002 0.0009 0.0025 0.0147 0.0016 0.0025 0.0056 0.0321 0.0004 0.0416 0.0004 0.0009 0.0001 0.0013 0.0001
Nucleolus 0.0268 0.0148 0.0139 0.0033 0.1034 0.056 0.021 0.0104 0.0071 0.0078 0.0834 0.0366 0.0253 0.0067 0.0054 0.0059 0.0702 0.0313
Nucleus 0.8038 0.9846 0.929 0.9964 0.8886 0.9194 0.8644 0.9859 0.9642 0.8913 0.6942 0.9376 0.6916 0.9926 0.9229 0.9939 0.9262 0.9681
Peroxisomes 0.0044 0 0.0016 0 0 0.0016 0.0012 0 0.0011 0.0031 0.0041 0.0029 0.0068 0 0.0003 0 0 0
Punctate Nuclear 0.0045 0.0001 0.001 0 0.0051 0.0015 0.0077 0.0019 0.0031 0.0268 0.0639 0.0015 0.033 0.0001 0.0002 0 0.0004 0.0001
Vacuole 0.0031 0 0.0026 0 0.0002 0.0006 0.0025 0 0.0005 0.0006 0.0043 0.0007 0.0045 0 0.0003 0 0.0006 0
Vacuole Periphery 0.002 0 0.0012 0 0.0002 0.0005 0.0027 0 0.0003 0.0004 0.0039 0.0004 0.0072 0 0.0002 0 0.0003 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 66.9321 33.9829 39.2261 40.7646 48.2347 51.6326 34.3601 33.0022 32.6949 45.3097
Translational Efficiency 0.8776 1.0106 0.8513 0.8018 0.8233 0.8751 0.7406 0.6748 0.7224 0.777

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
460 1263 1637 1108 1646 991 73 908 2106 2254 1710 2016

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 742.40 937.27 1107.41 1089.51 836.11 926.41 1270.02 1111.41 815.64 932.50 1114.35 1099.37
Standard Deviation 105.13 167.82 150.11 226.10 123.67 149.14 179.71 230.61 125.96 159.97 155.02 228.40
Intensity Change Log 2 — 0.336268 0.576921 0.553411 — 0.147958 0.603087 0.410626 — 0.239591 0.590839 0.479543

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000264 0.000422 0.000370 0.000824 0.000273 0.000327 0.001280 0.000735 0.000271 0.000380 0.000409 0.000784
Bud Neck 0.002342 0.008147 0.013282 0.020987 0.004089 0.007812 0.025543 0.017130 0.003708 0.008000 0.013805 0.019250
Bud Site 0.000355 0.000819 0.000595 0.005410 0.000323 0.001224 0.002315 0.002452 0.000330 0.000997 0.000668 0.004078
Cell Periphery 0.000095 0.000207 0.000048 0.000206 0.000190 0.000468 0.000056 0.000127 0.000169 0.000322 0.000048 0.000170
Cytoplasm 0.000455 0.001594 0.000374 0.006057 0.000251 0.000290 0.000563 0.002046 0.000296 0.001021 0.000382 0.004251
Cytoplasmic Foci 0.000363 0.000117 0.000005 0.001905 0.000082 0.000444 0.000048 0.000717 0.000143 0.000261 0.000007 0.001370
Eisosomes 0.000020 0.000042 0.000041 0.000040 0.000040 0.000056 0.000047 0.000037 0.000036 0.000048 0.000041 0.000038
Endoplasmic Reticulum 0.003049 0.004803 0.001950 0.002360 0.006074 0.005789 0.003233 0.002153 0.005413 0.005236 0.002005 0.002267
Endosome 0.000220 0.000287 0.000037 0.004658 0.000124 0.000437 0.000102 0.002769 0.000145 0.000353 0.000040 0.003807
Golgi 0.000134 0.000076 0.000021 0.001524 0.000115 0.000733 0.000096 0.000598 0.000119 0.000365 0.000024 0.001107
Lipid Particles 0.000152 0.000067 0.000011 0.000439 0.000084 0.000602 0.000092 0.000969 0.000099 0.000302 0.000014 0.000678
Mitochondria 0.001541 0.001357 0.000689 0.002266 0.000384 0.001211 0.002435 0.003386 0.000636 0.001293 0.000763 0.002771
Mitotic Spindle 0.002554 0.000921 0.000024 0.018909 0.000145 0.003686 0.001406 0.006057 0.000671 0.002137 0.000083 0.013121
None 0.002606 0.002114 0.000586 0.008322 0.001382 0.001514 0.000803 0.002878 0.001650 0.001850 0.000595 0.005870
Nuclear Periphery 0.000177 0.000292 0.000079 0.001769 0.000113 0.000356 0.000312 0.001202 0.000127 0.000320 0.000089 0.001513
Nuclear Periphery Foci 0.000135 0.000167 0.000007 0.002323 0.000117 0.000280 0.000053 0.000760 0.000120 0.000217 0.000009 0.001619
Nucleolus 0.008647 0.007778 0.004395 0.008468 0.007877 0.008959 0.013233 0.005721 0.008045 0.008297 0.004773 0.007231
Nucleus 0.976399* 0.968378* 0.976938* 0.905559* 0.977648* 0.963676* 0.945731* 0.945592* 0.977375* 0.966311* 0.975606* 0.923590*
Peroxisomes 0.000171 0.000216 0.000119 0.000560 0.000146 0.000472 0.000796 0.000334 0.000152 0.000329 0.000147 0.000459
Vacuole 0.000211 0.001535 0.000362 0.005595 0.000443 0.000972 0.001683 0.003034 0.000392 0.001287 0.000418 0.004442
Vacuole Periphery 0.000113 0.000661 0.000068 0.001817 0.000100 0.000691 0.000175 0.001304 0.000103 0.000674 0.000072 0.001586

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.61 -2.87 -11.28 -10.49 -11.76 -1.26 -5.24 -7.12 -6.62 4.00 -3.69 -5.87 -13.08 -11.80 -8.69
Bud Neck -7.86 -12.68 -11.82 -8.01 -4.64 -3.53 -5.84 -7.31 -4.88 2.02 -6.56 -12.03 -13.02 -9.15 -4.12
Bud Site -3.18 -2.66 -5.37 -4.86 -5.10 -3.12 -4.20 -4.52 -2.49 1.30 -4.53 -6.09 -6.81 -5.46 -6.01
Cell Periphery -2.55 1.55 -2.05 0.86 -5.28 -1.24 2.34 1.39 1.54 -1.05 -1.48 3.91 0.48 1.69 -5.70
Cytoplasm -1.95 0.35 -4.32 -3.09 -4.36 -0.21 -2.45 -3.15 -3.10 -1.65 -2.11 -0.45 -5.20 -3.89 -5.02
Cytoplasmic Foci 0.54 1.03 -2.17 -3.37 -3.88 -1.48 -0.31 -1.97 -0.52 -1.92 -1.00 1.43 -3.66 -2.99 -4.51
Eisosomes -4.00 -4.62 -4.58 0.23 -0.15 -2.73 -3.45 0.86 3.33 3.89 -3.33 -2.31 -1.46 2.62 1.19
Endoplasmic Reticulum -4.82 3.43 1.37 8.76 -3.84 0.54 0.72 9.89 8.40 2.92 0.49 10.94 9.67 12.02 -2.67
Endosome -0.68 1.91 -3.49 -3.41 -3.71 -2.90 -2.32 -2.30 -1.97 -2.15 -3.17 3.43 -4.20 -3.86 -4.35
Golgi 0.53 1.12 -2.81 -3.09 -3.26 -1.37 -1.46 -1.68 0.49 -0.97 -1.16 1.10 -3.21 -1.60 -3.93
Lipid Particles 0.57 1.17 -1.05 -1.81 -2.34 -1.17 -2.07 -1.07 -0.25 -0.55 -1.05 1.49 -1.42 -0.65 -1.66
Mitochondria 0.17 0.95 -1.02 -1.54 -8.87 -1.50 -8.01 -4.15 -2.38 0.49 -1.37 -1.11 -5.70 -2.78 -5.77
Mitotic Spindle 0.86 1.57 -4.40 -5.33 -5.80 -2.22 -1.99 -3.26 -1.46 0.82 -1.69 0.30 -6.22 -5.19 -6.33
None 0.64 3.05 -3.52 -4.38 -6.02 -0.29 1.10 -2.17 -1.70 -2.70 -0.61 4.10 -5.04 -4.61 -6.86
Nuclear Periphery -1.59 2.28 -3.62 -3.28 -3.88 -2.56 -3.60 -2.65 -1.98 0.03 -3.50 -0.18 -4.56 -3.77 -4.54
Nuclear Periphery Foci -0.51 2.32 -3.36 -3.27 -3.64 -0.91 -1.06 -2.33 -1.69 -1.35 -0.84 1.93 -3.92 -3.67 -4.46
Nucleolus 0.52 2.46 -0.60 -1.63 -5.07 -0.81 -2.18 -0.10 0.50 2.08 -0.25 3.93 -0.97 -0.71 -4.18
Nucleus 1.87 0.13 9.44 9.05 10.92 3.63 4.54 6.62 3.56 -1.82 4.58 2.30 12.51 9.27 11.58
Peroxisomes -1.21 1.33 -2.75 -2.41 -3.15 -1.16 -3.59 -2.65 0.51 3.03 -1.43 -1.04 -3.65 -0.81 -3.33
Vacuole -2.25 -2.81 -4.80 -2.95 -4.61 -1.93 -3.62 -3.28 -2.61 1.66 -2.52 -2.49 -5.69 -3.85 -5.28
Vacuole Periphery -1.69 1.20 -3.56 -1.47 -3.69 -1.54 -3.88 -2.70 -0.82 -2.10 -2.31 1.19 -4.48 -1.63 -4.56
Standard name
Human Ortholog
Description Snf2p family member with ATP-dependent chromatin remodeling activity; has a role in silencing at the mating type locus, telomeres and centromeres; enriched at centromeres and is required for correct chromatin structure around centromeres, as well as at the boundary element of the silent HMR; recruited to DNA double-strand breaks (DSBs) where it promotes 5' strand resection of DSBs; potential Cdc28p substrate
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Fun30

Fun30


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fun30-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available