Standard name
Human Ortholog
Description Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has similarity to Alr1p and Alr2p, which mediate influx of Mg2+ and other divalent cations; localizes to sites of contact between the vacuole and mitochondria (vCLAMPs)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0.12 0.06 0.05 0.11 0.09 0.07 0.07
Bud 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0.06 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.05 0 0 0 0 0 0 0.14 0.09 0.11 – 0 0.19 0 0 0 0 0.06 0.06
Cytoplasm 0.68 0.82 0.77 0.82 0.87 0.86 0.84 0.79 0.85 0.81 0.72 0.71 0.8 0.75 0.82 – 0.71 0.75 0.14 0.21 0.1 0.07 0.1 0.11
Endoplasmic Reticulum 0.25 0.18 0.14 0 0.06 0 0 0 0 0.05 0 0.07 0.13 0.19 0.14 – 0.17 0 0.5 0.43 0.53 0.42 0.48 0.43
Endosome 0 0 0 0.05 0 0 0.06 0.09 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.07 0 0.05 0.07 0.06 0.1 0.15 0.27 0.23 0 0 0 – 0.12 0 0.09 0.08 0.1 0.17 0.13 0.11
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 – 0 0 0.1 0.11 0.05 0.11 0.09 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 9 1 11 9 4 2 3 3 1 2 3 5 0 3 5 – 1 2 15 10 38 19 22 22
Bud 3 4 6 13 6 6 5 8 10 3 11 13 1 3 1 – 0 0 1 1 0 1 3 4
Bud Neck 0 0 0 0 0 1 1 0 1 0 0 0 0 0 1 – 1 0 10 13 11 7 7 10
Bud Site 0 0 2 1 0 0 0 0 0 0 0 1 0 0 0 – 0 0 – – – – – –
Cell Periphery 6 4 9 9 8 17 12 11 14 6 14 13 12 11 18 – 0 3 4 3 9 8 19 19
Cytoplasm 150 156 223 213 246 319 377 344 328 252 253 271 69 88 141 – 17 12 36 45 35 14 33 33
Endoplasmic Reticulum 54 35 40 9 18 16 15 14 16 16 15 25 11 22 24 – 4 0 134 93 187 87 164 137
Endosome 5 1 8 12 5 8 25 38 13 10 8 7 0 0 0 – 0 0 6 5 8 4 4 6
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 – 0 0 2 0 4 2 9 1
Mitochondria 3 5 8 18 6 20 33 27 40 48 93 89 0 0 0 – 3 0 22 16 34 35 44 36
Nucleus 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 1 0 0 1 1 0 2 1 1 0 1 0 0 0 – 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 1 5 1 1 0 3 0 0 0 0 0 0 0 – 0 0 0 1 1 1 0 0
SpindlePole 2 0 2 2 0 0 0 3 0 1 2 2 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 3 1 6 0 11 10 23 18 9 14 10 12 2 0 0 – 0 0 27 23 16 23 30 41
Unique Cell Count 219 190 291 260 284 372 451 437 385 313 350 382 86 117 171 24 16 269 217 356 210 346 322
Labelled Cell Count 235 208 316 291 307 402 495 473 433 353 409 439 95 127 191 26 17 269 217 356 210 346 322


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 5.8 4.9 5.0 5.4 4.6 4.7 5.0 4.6 4.4 4.3 4.5 6.5 6.2 7.0 4.7 5.5 6.6 7.0 7.4 7.3
Std Deviation (1e-4) 0.8 0.8 1.1 1.2 1.7 1.3 1.3 1.3 1.0 1.2 0.8 0.9 0.6 0.7 0.7 1.0 1.6 1.2 1.5 1.7 1.3
Intensity Change (Log2) – – – 0.02 0.13 -0.09 -0.08 0.03 -0.12 -0.16 -0.19 -0.13 0.39 0.34 0.5 -0.06 0.16 0.42 0.5 0.59 0.57


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.2 -1.8 0 0 -3.0 0 0 0 0 0 0 -0.5 – 0 0
Bud 1.9 0 0 0 -0.2 0 0 0 0 0 0 0 – 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Cell Periphery 0.2 -0.2 0 0 -0.5 0 0 0 0 0 2.7 3.3 – 0 0
Cytoplasm 1.5 3.1 3.0 2.4 0.7 2.8 1.2 -1.3 -1.7 0.7 -0.3 1.5 – -0.6 -0.2
Endoplasmic Reticulum -4.2 -2.9 0 0 -5.3 0 0 0 0 -0.2 1.3 0.1 – 0 0
Endosome 1.2 -0.8 0 0 3.2 0 0 0 0 0 0 0 – 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Mitochondria 2.3 -0.5 0 0 2.1 0 0 0 0 0 0 0 – 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Vacuole 0 1.3 0.5 2.1 1.5 0.2 1.7 0.6 0.9 0 0 0 – 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.096 0.0182 0.0512 0.0023 0.0187 0.0155 – – – – – – 0.019 0.0057 0.0126 0.0049 0.0694 0.0031
Bud 0.001 0.0003 0.0012 0.0018 0.0005 0.0002 – – – – – – 0.0002 0 0.0003 0.0013 0.0003 0.0001
Bud Neck 0.0089 0.001 0.0099 0.0014 0.0075 0.0086 – – – – – – 0.0006 0.0006 0.0041 0.0049 0.0157 0.0032
Bud Periphery 0.0016 0.0004 0.002 0.0079 0.0008 0.0006 – – – – – – 0.0005 0.0001 0.0012 0.0067 0.0006 0.0003
Bud Site 0.0162 0.0121 0.0167 0.0026 0.0029 0.0007 – – – – – – 0.0019 0.0011 0.0047 0.0041 0.0013 0.0001
Cell Periphery 0.0034 0.006 0.0059 0.0028 0.0012 0.0026 – – – – – – 0.0121 0.0072 0.0192 0.0315 0.0033 0.0037
Cytoplasm 0.3403 0.607 0.5294 0.5208 0.5594 0.6533 – – – – – – 0.4857 0.5218 0.6021 0.4416 0.5295 0.6518
Cytoplasmic Foci 0.0455 0.0247 0.0421 0.0203 0.0413 0.0336 – – – – – – 0.0113 0.021 0.017 0.0413 0.0478 0.0122
Eisosomes 0.0015 0.0003 0.001 0.0002 0.0005 0.0002 – – – – – – 0.001 0.0002 0.0015 0.0003 0.0003 0.0002
Endoplasmic Reticulum 0.1648 0.1435 0.136 0.2315 0.0916 0.1388 – – – – – – 0.275 0.2017 0.1777 0.2374 0.0628 0.1697
Endosome 0.0429 0.0206 0.0361 0.0476 0.0593 0.0295 – – – – – – 0.0143 0.0085 0.0161 0.1004 0.0119 0.0085
Golgi 0.0178 0.0057 0.0176 0.0057 0.0109 0.0083 – – – – – – 0.008 0.0075 0.007 0.0201 0.0061 0.0039
Lipid Particles 0.0536 0.0208 0.0254 0.0185 0.036 0.0199 – – – – – – 0.0469 0.0405 0.016 0.0435 0.0307 0.0063
Mitochondria 0.0057 0.0006 0.0053 0.0285 0.0041 0.0024 – – – – – – 0.0209 0.0001 0.0126 0.0099 0.0008 0.0005
None 0.1252 0.1186 0.0829 0.0874 0.1036 0.0633 – – – – – – 0.0834 0.1766 0.0927 0.0199 0.2103 0.1313
Nuclear Periphery 0.0231 0.0042 0.0097 0.0046 0.0106 0.0091 – – – – – – 0.0071 0.0027 0.0035 0.0045 0.0011 0.0013
Nucleolus 0.0014 0.0004 0.0008 0.0004 0.0025 0.0002 – – – – – – 0.0003 0.0001 0.0001 0.0002 0.0002 0
Nucleus 0.0089 0.0022 0.0021 0.002 0.0083 0.002 – – – – – – 0.0012 0.0006 0.0011 0.0013 0.0009 0.0006
Peroxisomes 0.0205 0.0031 0.0111 0.0007 0.0093 0.0029 – – – – – – 0.0016 0.0003 0.0004 0.0026 0.0024 0.0001
Punctate Nuclear 0.0147 0.0022 0.0082 0.001 0.023 0.0023 – – – – – – 0.0009 0.0007 0.0007 0.0006 0.0008 0.0003
Vacuole 0.0053 0.0078 0.0043 0.0101 0.007 0.0055 – – – – – – 0.0072 0.0029 0.0092 0.0204 0.0035 0.0027
Vacuole Periphery 0.0019 0.0004 0.001 0.0021 0.0011 0.0006 – – – – – – 0.0009 0.0001 0.0004 0.0025 0.0003 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.3901 14.5753 17.3551 19.8433 17.6983 15.9166 19.5352 21.3966 21.7944 23.1223
Translational Efficiency 0.6278 0.8865 0.6243 0.6715 0.6511 0.9397 0.617 0.5867 0.5743 0.4924

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
746 802 142 997 146 1368 2442 1963 892 2170 2584 2960

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 797.63 772.05 1090.82 972.75 625.32 788.30 870.90 891.90 769.43 782.29 882.99 919.13
Standard Deviation 97.87 102.02 106.65 128.75 65.74 111.82 101.02 115.96 113.06 108.59 113.05 126.33
Intensity Change Log 2 — -0.047025 0.451621 0.286349 — 0.334150 0.477912 0.512287 — 0.132985 0.463234 0.390020

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001150 0.006800 0.002758 0.009058 0.004010 0.003444 0.002183 0.005018 0.001619 0.004684 0.002215 0.006379
Bud Neck 0.013193 0.026165 0.003455 0.012345 0.055747 0.023821 0.019074 0.016032 0.020158 0.024687 0.018216 0.014790
Bud Site 0.019415 0.024328 0.019373 0.039259 0.016115 0.014041 0.035121 0.043561 0.018874 0.017843 0.034256 0.042112
Cell Periphery 0.001759 0.002132 0.001055 0.003350 0.023925 0.003114 0.001670 0.001820 0.005387 0.002751 0.001636 0.002335
Cytoplasm 0.385277* 0.263116* 0.276212* 0.298423* 0.353920* 0.337112* 0.448534* 0.399906* 0.380144* 0.309764* 0.439065* 0.365724*
Cytoplasmic Foci 0.139567 0.288629* 0.123178 0.072808 0.120320 0.237521* 0.068953 0.086124 0.136416 0.256410* 0.071933 0.081639
Eisosomes 0.001100 0.003311 0.000298 0.002316 0.017573 0.004245 0.001259 0.001590 0.003796 0.003900 0.001206 0.001835
Endoplasmic Reticulum 0.015686 0.063022 0.051399 0.068791 0.072890 0.059413 0.048431 0.046856 0.025049 0.060747 0.048594 0.054244
Endosome 0.017409 0.050813 0.038627 0.008596 0.020253 0.040569 0.006961 0.010238 0.017875 0.044355 0.008701 0.009685
Golgi 0.004573 0.043883 0.000996 0.002261 0.051649 0.044760 0.002092 0.003029 0.012278 0.044436 0.002032 0.002771
Lipid Particles 0.035728 0.073972 0.030864 0.023557 0.107705* 0.081919 0.024832 0.028865 0.047509 0.078982 0.025163 0.027077
Mitochondria 0.004338 0.010894 0.000080 0.000519 0.046780 0.015455 0.000814 0.001030 0.011285 0.013769 0.000774 0.000858
Mitotic Spindle 0.002293 0.000829 0.000457 0.004584 0.000091 0.001223 0.001069 0.002433 0.001932 0.001077 0.001036 0.003157
None 0.001774 0.001871 0.000224 0.001228 0.000436 0.001279 0.001042 0.000895 0.001555 0.001498 0.000997 0.001007
Nuclear Periphery 0.001913 0.000846 0.002714 0.001058 0.000961 0.000635 0.001778 0.000903 0.001757 0.000713 0.001830 0.000955
Nuclear Periphery Foci 0.003547 0.002794 0.090694 0.023160 0.000928 0.001423 0.008940 0.009390 0.003118 0.001930 0.013432 0.014028
Nucleolus 0.000744 0.000519 0.000281 0.000175 0.000735 0.000542 0.000446 0.000326 0.000742 0.000533 0.000437 0.000275
Nucleus 0.163838* 0.025940 0.048780 0.098617 0.025143 0.036596 0.231267* 0.123191 0.141137* 0.032658 0.221239* 0.114914
Peroxisomes 0.005036 0.019663 0.001089 0.002280 0.058608 0.016304 0.002519 0.004657 0.013804 0.017545 0.002440 0.003857
Vacuole 0.180829 0.086038 0.307009* 0.326985* 0.018177 0.072530 0.092757 0.213143* 0.154207 0.077523 0.104530 0.251487*
Vacuole Periphery 0.000834 0.004436 0.000455 0.000630 0.004031 0.004053 0.000258 0.000992 0.001357 0.004194 0.000268 0.000870

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.54 -1.34 -6.03 -0.90 -3.44 0.42 1.40 -0.58 -2.13 -5.79 -5.52 -1.74 -8.08 -1.85 -7.17
Bud Neck -5.37 6.35 0.52 6.50 -9.28 2.63 3.04 3.34 4.79 2.12 -1.78 0.74 2.31 7.56 2.60
Bud Site -1.32 -0.03 -3.89 -3.22 -2.90 0.55 -4.24 -5.62 -8.91 -1.75 0.26 -4.10 -6.07 -9.02 -1.88
Cell Periphery -1.01 2.57 -3.28 -1.58 -5.34 3.59 3.85 3.85 2.62 -0.30 2.43 3.70 3.20 1.65 -3.08
Cytoplasm 10.06 4.93 8.43 -1.70 -0.23 0.84 -4.57 -1.76 -6.45 7.84 6.82 -6.84 2.21 -6.64 13.10
Cytoplasmic Foci -17.88 1.74 12.13 30.44 7.14 -10.56 4.93 3.43 29.11 -4.90 -20.26 12.51 11.32 41.24 -2.43
Eisosomes -6.76 6.94 -4.02 3.56 -8.03 3.69 4.55 4.49 6.40 -1.83 -0.19 4.08 3.36 7.39 -3.76
Endoplasmic Reticulum -8.57 -3.86 -10.08 0.74 -1.12 1.08 2.02 2.39 3.58 1.37 -8.91 -6.71 -7.03 3.35 -0.10
Endosome -11.15 -5.97 5.96 16.15 9.54 -5.90 4.31 3.38 18.11 -4.86 -13.67 6.26 6.07 24.14 -0.81
Golgi -13.63 2.53 1.77 15.84 -1.20 1.26 9.71 9.56 20.36 -2.07 -14.74 6.69 6.34 25.80 -1.26
Lipid Particles -8.19 1.23 4.38 12.92 2.20 2.10 6.90 6.67 16.74 -1.45 -8.16 6.39 6.38 20.98 -0.21
Mitochondria -3.17 2.76 2.50 6.73 -4.64 3.47 5.18 5.16 8.61 -1.11 -1.15 5.25 5.23 10.75 -0.24
Mitotic Spindle 1.23 1.68 -1.29 -2.98 -3.54 -3.23 -2.79 -3.64 -1.50 -1.88 0.92 1.05 -0.87 -3.13 -3.27
None -0.16 2.65 0.88 0.87 -1.34 -2.14 -2.99 -1.80 1.04 0.76 0.01 1.12 1.13 1.34 0.23
Nuclear Periphery 3.36 -0.96 3.12 -1.15 2.57 1.29 -3.01 0.06 -3.90 5.89 4.41 -0.20 3.40 -3.14 6.20
Nuclear Periphery Foci 1.74 -9.73 -12.20 -12.93 7.54 -1.51 -12.61 -13.26 -13.97 -0.70 3.75 -11.94 -15.02 -18.49 -0.29
Nucleolus 1.04 2.50 3.19 5.30 1.80 0.49 0.76 1.08 2.82 3.67 1.15 1.86 2.90 4.85 5.39
Nucleus 19.86 10.97 8.76 -17.22 -5.35 -1.97 -30.48 -15.44 -24.26 21.29 17.73 -11.41 4.08 -29.72 23.52
Peroxisomes -10.61 5.85 4.28 14.15 -2.56 4.26 5.70 5.51 11.02 -4.47 -1.95 6.21 5.56 16.85 -3.56
Vacuole 12.15 -6.55 -15.31 -30.71 -2.91 -12.05 -18.22 -35.59 -28.38 -24.55 11.94 7.49 -13.64 -40.12 -32.46
Vacuole Periphery -7.30 1.77 1.09 7.78 -0.18 -0.02 6.54 5.09 6.57 -2.89 -7.75 5.66 2.31 9.60 -3.01
Standard name
Human Ortholog
Description Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has similarity to Alr1p and Alr2p, which mediate influx of Mg2+ and other divalent cations; localizes to sites of contact between the vacuole and mitochondria (vCLAMPs)
Localization
Cell Percentages ER (34%), vacuole (1%), mixed (41%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Mnr2

Mnr2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mnr2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available