Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of the BLOC-1 complex involved in endosomal maturation; interacts with Msb3p; green fluorescent protein (GFP)-fusion protein localizes to the endosome |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 13 | 4 | 4 | 1 | 0 | 3 | – | 4 | 1 | 0 | 3 | 2 | 4 | 3 | 1 | 2 | 1 | 5 | 4 | 1 | 3 | 0 | 0 | 1 |
Bud | 2 | 1 | 16 | 6 | 8 | 11 | – | 34 | 29 | 19 | 24 | 21 | 2 | 5 | 6 | 0 | 1 | 1 | 2 | 1 | 1 | 0 | 1 | 2 |
Bud Neck | 0 | 0 | 2 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 1 | – | 3 | 2 | 0 | 1 | 6 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 109 | 45 | 53 | 58 | 67 | 142 | – | 280 | 252 | 232 | 152 | 219 | 74 | 54 | 65 | 86 | 103 | 80 | 37 | 12 | 22 | 18 | 23 | 17 |
Endoplasmic Reticulum | 10 | 11 | 7 | 3 | 0 | 13 | – | 3 | 6 | 4 | 4 | 3 | 6 | 2 | 2 | 5 | 4 | 9 | 2 | 0 | 2 | 0 | 0 | 5 |
Endosome | 204 | 105 | 137 | 124 | 224 | 148 | – | 174 | 72 | 80 | 26 | 61 | 243 | 221 | 271 | 55 | 58 | 51 | 326 | 159 | 224 | 122 | 214 | 220 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 2 | 2 | 1 | 2 | 2 |
Mitochondria | 3 | 4 | 27 | 31 | 11 | 88 | – | 180 | 304 | 203 | 161 | 249 | 1 | 3 | 0 | 5 | 3 | 1 | 2 | 1 | 2 | 0 | 1 | 1 |
Nucleus | 1 | 1 | 0 | 1 | 1 | 0 | – | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 2 | 0 | 0 | 0 | 0 | – | 3 | 0 | 1 | 2 | 0 | 0 | 1 | 2 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 1 |
Nucleolus | 9 | 14 | 13 | 11 | 6 | 5 | – | 13 | 2 | 1 | 1 | 0 | 9 | 13 | 13 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 3 | 0 | 16 | 3 | – | 17 | 1 | 2 | 2 | 6 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 27 | 10 | 33 | 6 | 32 | 10 | – | 26 | 12 | 10 | 11 | 12 | 42 | 34 | 46 | 12 | 14 | 16 | 20 | 13 | 25 | 14 | 14 | 13 |
Vac/Vac Membrane | 71 | 47 | 31 | 1 | 32 | 12 | – | 24 | 18 | 18 | 10 | 9 | 40 | 34 | 40 | 16 | 10 | 16 | 12 | 10 | 3 | 7 | 12 | 10 |
Unique Cell Count | 358 | 188 | 260 | 208 | 306 | 364 | 599 | 571 | 462 | 308 | 467 | 334 | 296 | 354 | 154 | 169 | 151 | 419 | 209 | 296 | 170 | 276 | 281 | |
Labelled Cell Count | 449 | 245 | 326 | 242 | 397 | 436 | 765 | 699 | 571 | 397 | 591 | 422 | 371 | 447 | 185 | 197 | 182 | 419 | 209 | 296 | 170 | 276 | 281 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.2 | 8.3 | 6.0 | 6.5 | 7.1 | 5.8 | – | 5.6 | 4.9 | 5.3 | 5.0 | 4.9 | 8.2 | 8.0 | 8.0 | 8.7 | 8.5 | 8.5 | 7.8 | 8.3 | 8.7 |
Std Deviation (1e-4) | 0.9 | 1.0 | 1.5 | 1.8 | 1.6 | 1.2 | – | 1.5 | 0.9 | 1.1 | 1.3 | 0.9 | 1.2 | 1.1 | 1.0 | 1.8 | 2.1 | 2.1 | 1.4 | 1.2 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.11 | 0.22 | -0.06 | – | -0.11 | -0.3 | -0.18 | -0.28 | -0.3 | 0.44 | 0.41 | 0.41 | 0.53 | 0.49 | 0.48 | 0.37 | 0.46 | 0.53 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | -1.7 | -2.1 | -1.9 | – | -0.3 | -0.6 | -1.2 | 0.8 | -1.0 | -3.9 | -2.8 | -2.9 | -3.1 | -2.9 | -2.7 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.9 | 0.4 | 4.9 | – | 7.3 | 6.6 | 7.9 | 7.2 | 7.1 | 0.5 | -0.6 | -0.6 | 7.4 | 8.5 | 6.8 |
Endoplasmic Reticulum | 0 | 0 | 0.6 | – | 0 | 0 | 0 | -1.2 | 0 | -0.7 | 0 | 0 | 0 | 0 | 1.7 |
Endosome | 1.5 | 5.1 | -3.0 | – | -6.6 | -12.3 | -10.0 | -11.6 | -11.5 | 5.1 | 5.4 | 6.2 | -3.3 | -3.7 | -3.7 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1.5 | -3.2 | 4.4 | – | 6.2 | 11.7 | 9.3 | 10.6 | 11.4 | -5.8 | -4.9 | -6.2 | -2.6 | -3.4 | -3.8 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0.1 | -2.0 | -2.7 | – | -2.2 | 0 | -4.5 | -3.6 | 0 | -1.5 | -0.3 | -0.8 | -1.9 | -3.0 | -2.3 |
Peroxisomes | 0 | 2.7 | 0 | – | 1.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | -3.8 | -0.8 | -4.8 | – | -4.4 | -6.3 | -5.7 | -4.1 | -5.4 | 0 | -0.4 | 0.1 | -1.5 | -1.4 | -0.6 |
Vacuole | -4.9 | -0.6 | -4.2 | – | -4.4 | -5.0 | -4.1 | -4.0 | -5.7 | 0 | -0.2 | -0.2 | -0.5 | -2.1 | -0.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.1036 | 3.1083 | 2.51 | 1.6916 | 1.6845 | 2.4799 | 2.0216 | 2.6783 | 2.268 | 1.9055 | 2.0474 | 2.3056 | 2.9924 | 3.7596 | 3.5309 | 3.1633 | 3.1952 | 3.5264 |
Actin | 0.0108 | 0.0001 | 0.0016 | 0.0122 | 0.0201 | 0.0019 | 0.037 | 0.0001 | 0.024 | 0.0074 | 0.0002 | 0.0002 | 0.0147 | 0.0014 | 0.0072 | 0.0002 | 0 | 0.0005 |
Bud | 0.0003 | 0 | 0.0033 | 0.0007 | 0.0002 | 0.0006 | 0.0006 | 0.0003 | 0.0003 | 0.0008 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0 | 0.0001 |
Bud Neck | 0.0008 | 0.0002 | 0.0006 | 0.0007 | 0.0008 | 0.0008 | 0.0018 | 0.0003 | 0.0004 | 0.0008 | 0.0004 | 0.0004 | 0.0004 | 0.0004 | 0.0015 | 0.0004 | 0.0002 | 0.0005 |
Bud Periphery | 0.0014 | 0 | 0.0013 | 0.0006 | 0.0002 | 0.0004 | 0.0007 | 0.0001 | 0.0003 | 0.0011 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0 |
Bud Site | 0.0019 | 0.0003 | 0.0025 | 0.0034 | 0.001 | 0.0002 | 0.0032 | 0.0009 | 0.0034 | 0.0047 | 0.0004 | 0.0002 | 0.0007 | 0.0027 | 0.0037 | 0.0008 | 0.0001 | 0.0001 |
Cell Periphery | 0.002 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0006 | 0.0005 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.1718 | 0.1615 | 0.1027 | 0.1932 | 0.1193 | 0.1136 | 0.112 | 0.1455 | 0.0912 | 0.1378 | 0.0132 | 0.0741 | 0.1458 | 0.1859 | 0.1283 | 0.1745 | 0.0736 | 0.1012 |
Cytoplasmic Foci | 0.317 | 0.4331 | 0.3668 | 0.3576 | 0.3555 | 0.4596 | 0.277 | 0.424 | 0.3471 | 0.3094 | 0.4581 | 0.4372 | 0.3419 | 0.4239 | 0.4109 | 0.3885 | 0.4657 | 0.4163 |
Eisosomes | 0.0008 | 0 | 0 | 0.0001 | 0.0003 | 0 | 0.0003 | 0 | 0.0003 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0.0003 | 0 | 0 |
Endoplasmic Reticulum | 0.0088 | 0.0036 | 0.0019 | 0.0027 | 0.0048 | 0.0018 | 0.0047 | 0.0012 | 0.0063 | 0.0171 | 0.0004 | 0.0011 | 0.0038 | 0.003 | 0.0024 | 0.0024 | 0.001 | 0.0018 |
Endosome | 0.3417 | 0.3335 | 0.4279 | 0.3155 | 0.3292 | 0.3411 | 0.3976 | 0.3645 | 0.406 | 0.3662 | 0.3869 | 0.3874 | 0.3454 | 0.3209 | 0.3382 | 0.3311 | 0.417 | 0.3717 |
Golgi | 0.0172 | 0.0124 | 0.0297 | 0.0299 | 0.0319 | 0.0201 | 0.0249 | 0.015 | 0.0335 | 0.0294 | 0.038 | 0.0256 | 0.0202 | 0.018 | 0.0224 | 0.0224 | 0.013 | 0.0185 |
Lipid Particles | 0.0386 | 0.0135 | 0.0245 | 0.0095 | 0.0195 | 0.0079 | 0.0393 | 0.01 | 0.0181 | 0.0155 | 0.0103 | 0.0146 | 0.0207 | 0.0095 | 0.0086 | 0.0078 | 0.0028 | 0.0241 |
Mitochondria | 0.0029 | 0.0002 | 0.001 | 0.0035 | 0.0112 | 0.0016 | 0.0086 | 0.0003 | 0.0075 | 0.0432 | 0.0013 | 0.0005 | 0.003 | 0.0014 | 0.0006 | 0.0008 | 0.0003 | 0.0005 |
None | 0.0379 | 0.0247 | 0.0065 | 0.0075 | 0.0283 | 0.0054 | 0.0185 | 0.0145 | 0.0222 | 0.0075 | 0.0565 | 0.0096 | 0.0374 | 0.0037 | 0.0138 | 0.0452 | 0.0032 | 0.0177 |
Nuclear Periphery | 0.0047 | 0.001 | 0.0022 | 0.0008 | 0.0107 | 0.0045 | 0.0074 | 0.0004 | 0.0007 | 0.003 | 0.0002 | 0.0003 | 0.0055 | 0.001 | 0.001 | 0.0008 | 0.0004 | 0.0042 |
Nucleolus | 0.0012 | 0.0001 | 0.0005 | 0.0002 | 0.0005 | 0.0001 | 0.0012 | 0.0001 | 0.0001 | 0.001 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0005 |
Nucleus | 0.0028 | 0.0004 | 0.0019 | 0.0009 | 0.0035 | 0.0009 | 0.0031 | 0.0005 | 0.0003 | 0.0017 | 0.0002 | 0.0003 | 0.0014 | 0.001 | 0.001 | 0.0006 | 0.0003 | 0.0035 |
Peroxisomes | 0.0209 | 0.0073 | 0.013 | 0.0515 | 0.0512 | 0.0329 | 0.0318 | 0.0127 | 0.0305 | 0.0288 | 0.0282 | 0.0415 | 0.0374 | 0.0119 | 0.0502 | 0.0193 | 0.0179 | 0.0257 |
Punctate Nuclear | 0.0098 | 0.002 | 0.0059 | 0.0065 | 0.0083 | 0.0028 | 0.0173 | 0.0015 | 0.0011 | 0.0022 | 0.0019 | 0.0018 | 0.0123 | 0.0063 | 0.0028 | 0.0013 | 0.0008 | 0.0078 |
Vacuole | 0.0055 | 0.005 | 0.0046 | 0.0021 | 0.0024 | 0.0026 | 0.0106 | 0.0073 | 0.0048 | 0.0183 | 0.0025 | 0.0039 | 0.0075 | 0.0073 | 0.006 | 0.0025 | 0.003 | 0.0038 |
Vacuole Periphery | 0.0013 | 0.0009 | 0.0015 | 0.0009 | 0.001 | 0.0014 | 0.0023 | 0.0009 | 0.0011 | 0.0036 | 0.001 | 0.0011 | 0.0012 | 0.0009 | 0.0009 | 0.0008 | 0.0007 | 0.001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 9.679 | 17.3048 | 23.5417 | 15.5074 | 18.8238 | 19.3473 | 18.955 | 16.7298 | 24.1436 | 19.8972 |
Translational Efficiency | 2.6041 | 0.9891 | 0.9146 | 2.4242 | 1.2616 | 1.3712 | 1.4484 | 1.426 | 1.0644 | 1.2232 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
185 | 266 | 235 | 554 | 1578 | 602 | 2719 | 1364 | 1763 | 868 | 2954 | 1918 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 769.54 | 929.12 | 1136.52 | 964.58 | 755.74 | 885.11 | 979.56 | 1009.88 | 757.19 | 898.60 | 992.05 | 996.80 |
Standard Deviation | 128.18 | 131.75 | 134.44 | 162.86 | 103.60 | 125.05 | 124.90 | 125.50 | 106.53 | 128.75 | 132.67 | 138.86 |
Intensity Change Log 2 | — | 0.271869 | 0.562555 | 0.325905 | — | 0.227967 | 0.374244 | 0.418222 | — | 0.250283 | 0.472320 | 0.372384 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000228 | 0.000155 | 0.000232 | 0.000556 | 0.000137 | 0.000149 | 0.000105 | 0.000478 | 0.000147 | 0.000151 | 0.000115 | 0.000501 |
Bud Neck | 0.025158 | 0.006959 | 0.023581 | 0.020603 | 0.014214 | 0.006837 | 0.014737 | 0.013704 | 0.015362 | 0.006874 | 0.015440 | 0.015697 |
Bud Site | 0.018483 | 0.012132 | 0.018728 | 0.024622 | 0.010846 | 0.008538 | 0.011416 | 0.028990 | 0.011648 | 0.009639 | 0.011997 | 0.027728 |
Cell Periphery | 0.000079 | 0.000033 | 0.000162 | 0.000078 | 0.000031 | 0.000030 | 0.000016 | 0.000041 | 0.000036 | 0.000031 | 0.000027 | 0.000052 |
Cytoplasm | 0.096777 | 0.021732 | 0.026052 | 0.088105 | 0.063857 | 0.021906 | 0.031623 | 0.107802* | 0.067312 | 0.021853 | 0.031180 | 0.102113* |
Cytoplasmic Foci | 0.246448* | 0.144054 | 0.120549 | 0.121524 | 0.216108* | 0.204410* | 0.150281 | 0.178947 | 0.219292* | 0.185914* | 0.147916 | 0.162361 |
Eisosomes | 0.000085 | 0.000024 | 0.000089 | 0.000052 | 0.000031 | 0.000018 | 0.000010 | 0.000019 | 0.000036 | 0.000020 | 0.000016 | 0.000028 |
Endoplasmic Reticulum | 0.000359 | 0.000327 | 0.001175 | 0.001011 | 0.000280 | 0.000160 | 0.000239 | 0.000517 | 0.000289 | 0.000211 | 0.000313 | 0.000660 |
Endosome | 0.426791* | 0.646230* | 0.480559* | 0.356134* | 0.559308* | 0.642154* | 0.560889* | 0.325157* | 0.545403* | 0.643403* | 0.554499* | 0.334104* |
Golgi | 0.013268 | 0.031247 | 0.007301 | 0.007307 | 0.016382 | 0.031819 | 0.005701 | 0.007008 | 0.016055 | 0.031643 | 0.005828 | 0.007094 |
Lipid Particles | 0.011401 | 0.007576 | 0.008558 | 0.006591 | 0.008866 | 0.006769 | 0.003285 | 0.003620 | 0.009132 | 0.007017 | 0.003705 | 0.004478 |
Mitochondria | 0.009277 | 0.002555 | 0.000558 | 0.002556 | 0.003076 | 0.004739 | 0.001473 | 0.004989 | 0.003726 | 0.004070 | 0.001401 | 0.004286 |
Mitotic Spindle | 0.003539 | 0.022568 | 0.031802 | 0.034987 | 0.006492 | 0.012984 | 0.050484 | 0.060920 | 0.006182 | 0.015921 | 0.048998 | 0.053429 |
None | 0.002573 | 0.000204 | 0.000202 | 0.001596 | 0.000453 | 0.000084 | 0.000122 | 0.000240 | 0.000676 | 0.000121 | 0.000129 | 0.000632 |
Nuclear Periphery | 0.000136 | 0.000206 | 0.000855 | 0.000813 | 0.000147 | 0.000061 | 0.000623 | 0.000588 | 0.000146 | 0.000106 | 0.000641 | 0.000653 |
Nuclear Periphery Foci | 0.000552 | 0.003747 | 0.010869 | 0.008767 | 0.001614 | 0.002230 | 0.005173 | 0.003055 | 0.001502 | 0.002695 | 0.005627 | 0.004704 |
Nucleolus | 0.000606 | 0.001290 | 0.006572 | 0.016214 | 0.001396 | 0.000535 | 0.003542 | 0.001302 | 0.001313 | 0.000767 | 0.003783 | 0.005609 |
Nucleus | 0.015329 | 0.003108 | 0.058473 | 0.031802 | 0.002881 | 0.000371 | 0.005745 | 0.005173 | 0.004187 | 0.001209 | 0.009940 | 0.012864 |
Peroxisomes | 0.058342 | 0.018066 | 0.040427 | 0.036089 | 0.028433 | 0.026793 | 0.018738 | 0.034687 | 0.031571 | 0.024119 | 0.020464 | 0.035092 |
Vacuole | 0.067791 | 0.066435 | 0.159016 | 0.234917* | 0.062994 | 0.025831 | 0.132342 | 0.216418* | 0.063497 | 0.038274 | 0.134464 | 0.221761* |
Vacuole Periphery | 0.002778 | 0.011350 | 0.004239 | 0.005676 | 0.002454 | 0.003580 | 0.003456 | 0.006346 | 0.002488 | 0.005961 | 0.003518 | 0.006153 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.96 | -0.26 | -1.84 | -2.15 | -1.71 | -0.23 | 0.43 | -5.55 | -4.76 | -5.07 | -0.14 | 0.45 | -4.99 | -4.65 | -4.79 |
Bud Neck | 3.51 | 0.20 | 1.09 | -5.43 | 0.71 | 3.39 | -2.46 | -0.43 | -4.28 | 2.68 | 4.62 | -2.03 | -0.54 | -6.30 | 2.01 |
Bud Site | 1.22 | -0.24 | -1.66 | -3.21 | -1.61 | 1.19 | -1.56 | -7.83 | -7.08 | -6.98 | 1.05 | -1.37 | -8.36 | -7.55 | -7.65 |
Cell Periphery | 2.92 | -1.65 | 0.47 | -4.02 | 1.85 | 0.01 | 5.64 | -1.94 | -0.98 | -6.93 | 0.74 | 1.48 | -3.23 | -2.82 | -3.91 |
Cytoplasm | 5.53 | 5.23 | -1.35 | -9.23 | -8.87 | 8.26 | 4.23 | -14.35 | -20.67 | -18.65 | 9.78 | 5.58 | -13.71 | -22.43 | -19.80 |
Cytoplasmic Foci | 6.01 | 7.63 | 8.70 | 3.13 | 0.79 | 1.34 | 13.37 | 11.68 | 7.14 | 0.02 | 4.51 | 15.01 | 15.25 | 7.59 | 1.75 |
Eisosomes | 3.36 | -0.15 | 2.06 | -2.88 | 2.80 | 4.76 | 11.59 | 6.54 | -0.43 | -7.72 | 4.60 | 7.46 | 3.16 | -2.55 | -5.59 |
Endoplasmic Reticulum | 0.10 | -3.03 | -4.30 | -3.44 | 1.09 | 1.57 | -0.70 | -2.17 | -3.57 | -1.86 | 0.94 | -1.76 | -4.16 | -4.83 | -2.89 |
Endosome | -7.18 | -1.23 | 5.89 | 17.44 | 8.25 | -6.35 | 5.67 | 33.51* | 32.44 | 32.12* | -8.28 | 4.74 | 33.12 | 36.66 | 32.86* |
Golgi | -3.15 | 2.67 | 3.37 | 4.83 | 1.42 | -6.06 | 12.37 | 12.08 | 11.30 | 1.81 | -6.35 | 12.68 | 12.21 | 11.51 | 1.63 |
Lipid Particles | 0.69 | 0.57 | 1.18 | 0.52 | 0.93 | 1.70 | 6.56 | 6.23 | 2.61 | -0.83 | 1.55 | 5.93 | 5.35 | 2.10 | -1.35 |
Mitochondria | 1.53 | 2.01 | 1.72 | 0.84 | -2.28 | -0.88 | 2.73 | 0.25 | 0.96 | -1.51 | -0.22 | 3.32 | 1.36 | 1.09 | -1.68 |
Mitotic Spindle | -3.92 | -3.70 | -6.36 | -1.95 | -0.33 | -2.20 | -16.94 | -12.03 | -9.16 | 0.53 | -4.00 | -17.37 | -13.46 | -8.81 | 1.44 |
None | 1.02 | 1.04 | 0.48 | -2.48 | -2.59 | 2.44 | 1.79 | 0.59 | -3.97 | -2.80 | 2.02 | 1.80 | 0.03 | -3.68 | -3.32 |
Nuclear Periphery | -1.04 | -2.68 | -8.27 | -6.55 | -0.19 | 2.69 | -11.57 | -9.80 | -11.18 | -0.41 | 1.20 | -11.89 | -11.96 | -12.84 | -0.16 |
Nuclear Periphery Foci | -2.89 | -2.79 | -4.19 | -2.41 | 0.23 | -0.64 | -7.06 | -4.53 | -1.18 | 3.92 | -1.54 | -7.63 | -5.36 | -2.48 | 1.47 |
Nucleolus | -1.48 | -3.13 | -5.04 | -4.45 | -1.70 | 2.15 | -7.00 | -0.59 | -3.89 | 7.59 | 1.20 | -7.73 | -4.44 | -5.27 | 1.06 |
Nucleus | 2.03 | -3.43 | -2.10 | -6.07 | 2.39 | 4.43 | -5.11 | -7.20 | -11.14 | -2.23 | 3.06 | -5.51 | -7.88 | -10.46 | -2.41 |
Peroxisomes | 4.36 | 1.84 | 3.27 | -2.23 | 2.02 | 0.56 | 6.06 | 2.15 | 1.18 | -3.49 | 2.97 | 6.39 | 3.02 | -0.21 | -3.27 |
Vacuole | 0.18 | -5.84 | -15.44 | -16.95 | -7.76 | 8.86 | -18.71 | -31.02 | -36.24 | -18.38 | 6.01 | -19.58 | -35.42 | -38.80 | -20.71 |
Vacuole Periphery | -3.46 | -1.58 | -1.39 | 2.98 | 0.24 | -2.11 | -3.47 | -1.75 | -0.06 | -0.58 | -4.17 | -3.72 | -2.42 | 2.29 | -0.81 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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