Standard name | |
---|---|
Human Ortholog | |
Description | Transcriptional activator that enhances pseudohyphal growth; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; regulates expression of FLO11, an adhesin required for pseudohyphal filament formation; similar to StuA, an A. nidulans developmental regulator; potential Cdc28p substrate; PHD1 has a paralog, SOK2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 3 | 2 | 2 | 0 | 3 | 5 |
Bud | 0 | 0 | 0 | 0 | 0 | 4 | 7 | 3 | 14 | 14 | 16 | 10 | 0 | 0 | 1 | 0 | 0 | 0 | 11 | 5 | 4 | 15 | 12 | 20 |
Bud Neck | 0 | 0 | 1 | 0 | 2 | 1 | 2 | 5 | 3 | 5 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 1 | 3 | 8 | 10 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 5 | 5 | 10 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 175 | 81 | 55 | 140 | 283 | 279 | 311 | 298 | 172 | 213 | 109 | 150 | 61 | 227 | 455 | 214 | 92 | 154 | 146 | 79 | 43 | 34 | 190 | 298 |
Endoplasmic Reticulum | 1 | 3 | 1 | 1 | 1 | 2 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 20 | 4 | 14 | 1 | 1 | 1 | 2 | 1 | 4 |
Endosome | 7 | 9 | 4 | 12 | 13 | 7 | 4 | 4 | 8 | 6 | 3 | 6 | 27 | 32 | 7 | 3 | 4 | 10 | 22 | 8 | 4 | 2 | 10 | 13 |
Golgi | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 3 | 2 | 2 | 0 | 1 | 1 | 0 |
Mitochondria | 81 | 5 | 1 | 14 | 22 | 65 | 185 | 151 | 286 | 241 | 284 | 251 | 1 | 2 | 0 | 7 | 4 | 10 | 27 | 4 | 13 | 2 | 11 | 8 |
Nucleus | 63 | 55 | 70 | 73 | 23 | 25 | 42 | 44 | 99 | 99 | 90 | 103 | 132 | 8 | 2 | 7 | 7 | 12 | 96 | 93 | 75 | 163 | 67 | 35 |
Nuclear Periphery | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 3 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 6 | 8 | 5 | 15 | 6 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 2 | 0 | 1 | 2 | 1 | 1 | 5 | 5 | 8 | 6 | 4 | 4 | 4 | 0 | 0 | 1 | 0 | 1 | 36 | 17 | 9 | 33 | 34 | 49 |
Vac/Vac Membrane | 55 | 70 | 23 | 52 | 61 | 41 | 54 | 29 | 47 | 51 | 41 | 63 | 59 | 119 | 57 | 25 | 24 | 40 | 57 | 32 | 27 | 34 | 52 | 32 |
Unique Cell Count | 342 | 207 | 148 | 268 | 376 | 383 | 512 | 468 | 507 | 517 | 419 | 486 | 264 | 337 | 491 | 250 | 126 | 211 | 437 | 263 | 199 | 316 | 412 | 499 |
Labelled Cell Count | 386 | 225 | 156 | 296 | 408 | 426 | 616 | 545 | 643 | 640 | 561 | 598 | 284 | 388 | 527 | 282 | 137 | 249 | 437 | 263 | 199 | 316 | 412 | 499 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.9 | 5.0 | 6.0 | 5.8 | 5.4 | 4.5 | 4.1 | 4.5 | 4.0 | 4.0 | 3.7 | 4.0 | 6.8 | 6.5 | 6.1 | 5.1 | 6.0 | 5.8 | 6.2 | 6.1 | 6.3 |
Std Deviation (1e-4) | 0.8 | 0.8 | 1.2 | 2.0 | 1.7 | 1.6 | 1.6 | 1.6 | 1.6 | 1.1 | 1.2 | 0.9 | 2.2 | 1.8 | 1.8 | 1.1 | 1.2 | 1.5 | 1.6 | 1.8 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.05 | -0.14 | -0.39 | -0.53 | -0.4 | -0.57 | -0.57 | -0.68 | -0.57 | 0.18 | 0.13 | 0.03 | -0.22 | 0.0 | -0.04 | 0.05 | 0.04 | 0.09 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3.0 | 8.2 | 7.6 | 5.1 | 5.7 | -0.7 | 0.9 | -2.6 | -1.4 | -3.0 | 6.2 | 14.7 | 10.0 | 5.9 | 6.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 0 | 2.8 |
Endosome | 0.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 2.6 | 0 | 0 | 0 | 1.0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.4 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -4.1 | -11.1 | -11.0 | -11.2 | -10.3 | -6.8 | -6.9 | -6.0 | -6.2 | 0.5 | -12.4 | -15.8 | -10.9 | -7.7 | -9.2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 1.0 | 0.2 | -1.5 | -1.7 | -3.6 | -2.2 | -1.9 | -1.9 | -0.8 | 1.7 | 4.4 | -1.3 | -1.6 | 0.8 | 0.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0103 | 0.0012 | 0.0119 | 0.0077 | 0.0078 | 0.002 | 0.0243 | 0.0072 | 0.0058 | 0.0003 | 0.0402 | 0.0009 | 0.0563 | 0.0003 | 0.0295 | 0.0135 | 0.0001 | 0.0003 |
Bud | 0.0008 | 0.0009 | 0.0037 | 0.0013 | 0.0004 | 0.0004 | 0.0005 | 0.0005 | 0.0022 | 0.0003 | 0.0003 | 0.0014 | 0.0012 | 0.0006 | 0.0005 | 0.0179 | 0.0001 | 0.0006 |
Bud Neck | 0.0021 | 0.0009 | 0.0022 | 0.0054 | 0.0015 | 0.0032 | 0.0018 | 0.0007 | 0.0017 | 0.0018 | 0.0015 | 0.0035 | 0.0016 | 0.0005 | 0.0011 | 0.0053 | 0.0009 | 0.0035 |
Bud Periphery | 0.0012 | 0.0006 | 0.0012 | 0.0018 | 0.0008 | 0.0004 | 0.0009 | 0.0003 | 0.0013 | 0.0003 | 0.0003 | 0.0012 | 0.0017 | 0.0002 | 0.0003 | 0.0248 | 0 | 0.0003 |
Bud Site | 0.0075 | 0.006 | 0.0041 | 0.0009 | 0.0009 | 0.0005 | 0.0065 | 0.0041 | 0.0038 | 0.0006 | 0.0009 | 0.0007 | 0.007 | 0.001 | 0.0005 | 0.0436 | 0.0001 | 0.0006 |
Cell Periphery | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0005 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0006 | 0.0001 | 0.0001 | 0.0014 | 0 | 0 |
Cytoplasm | 0.1966 | 0.3315 | 0.2397 | 0.3035 | 0.2806 | 0.1788 | 0.1197 | 0.1929 | 0.1459 | 0.1318 | 0.0529 | 0.1429 | 0.0607 | 0.2019 | 0.1528 | 0.0625 | 0.004 | 0.1681 |
Cytoplasmic Foci | 0.0167 | 0.0063 | 0.0145 | 0.0139 | 0.0099 | 0.0071 | 0.0145 | 0.0018 | 0.0063 | 0.0119 | 0.0151 | 0.003 | 0.0644 | 0.0009 | 0.0009 | 0.0232 | 0 | 0.0004 |
Eisosomes | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0006 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0007 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0033 | 0.0026 | 0.0053 | 0.0027 | 0.002 | 0.0017 | 0.0056 | 0.0022 | 0.0021 | 0.0034 | 0.0011 | 0.004 | 0.0048 | 0.0022 | 0.0019 | 0.0075 | 0.0001 | 0.0013 |
Endosome | 0.0258 | 0.0045 | 0.0151 | 0.0055 | 0.0187 | 0.0062 | 0.028 | 0.0018 | 0.0163 | 0.0407 | 0.0089 | 0.0085 | 0.0712 | 0.0012 | 0.0013 | 0.0662 | 0.0001 | 0.0006 |
Golgi | 0.0054 | 0.0004 | 0.0055 | 0.0029 | 0.0083 | 0.0012 | 0.0142 | 0.0001 | 0.0046 | 0.0266 | 0.0374 | 0.0021 | 0.0302 | 0 | 0.0032 | 0.0361 | 0 | 0 |
Lipid Particles | 0.0086 | 0.0003 | 0.0061 | 0.0106 | 0.029 | 0.0007 | 0.0068 | 0.0001 | 0.0057 | 0.0183 | 0.0072 | 0.0004 | 0.024 | 0 | 0.0001 | 0.0073 | 0 | 0 |
Mitochondria | 0.0075 | 0.0012 | 0.0087 | 0.001 | 0.0087 | 0.0036 | 0.0186 | 0.0004 | 0.0224 | 0.0008 | 0.0053 | 0.0241 | 0.0433 | 0.0003 | 0.0022 | 0.0856 | 0.0002 | 0.0003 |
None | 0.0561 | 0.0622 | 0.0826 | 0.0903 | 0.0198 | 0.0377 | 0.0134 | 0.035 | 0.066 | 0.037 | 0.0007 | 0.0127 | 0.0015 | 0.0023 | 0.0293 | 0.0017 | 0 | 0.0074 |
Nuclear Periphery | 0.0174 | 0.0109 | 0.0142 | 0.0128 | 0.014 | 0.0091 | 0.0202 | 0.0124 | 0.0105 | 0.0251 | 0.0111 | 0.0078 | 0.006 | 0.0127 | 0.0115 | 0.013 | 0.0071 | 0.0132 |
Nucleolus | 0.0026 | 0.002 | 0.0023 | 0.0019 | 0.0018 | 0.0039 | 0.0032 | 0.0023 | 0.0019 | 0.004 | 0.0018 | 0.0026 | 0.0018 | 0.0014 | 0.0027 | 0.0021 | 0.0015 | 0.0027 |
Nucleus | 0.5811 | 0.5494 | 0.5551 | 0.484 | 0.5586 | 0.7012 | 0.6947 | 0.7276 | 0.6737 | 0.6017 | 0.7809 | 0.7559 | 0.5687 | 0.7677 | 0.7491 | 0.5508 | 0.9843 | 0.7907 |
Peroxisomes | 0.0233 | 0.0019 | 0.0061 | 0.0177 | 0.0212 | 0.0037 | 0.0015 | 0.0001 | 0.006 | 0.0004 | 0.0274 | 0.0009 | 0.0435 | 0 | 0.0002 | 0.0067 | 0 | 0 |
Punctate Nuclear | 0.0257 | 0.0147 | 0.0187 | 0.0347 | 0.013 | 0.0369 | 0.0181 | 0.0082 | 0.0213 | 0.0913 | 0.0023 | 0.0215 | 0.003 | 0.0053 | 0.0116 | 0.0065 | 0.0012 | 0.0088 |
Vacuole | 0.0057 | 0.0021 | 0.0021 | 0.0012 | 0.0018 | 0.0014 | 0.0052 | 0.0018 | 0.0014 | 0.0025 | 0.0043 | 0.0045 | 0.0054 | 0.001 | 0.001 | 0.0202 | 0.0002 | 0.0009 |
Vacuole Periphery | 0.0019 | 0.0004 | 0.0005 | 0.0003 | 0.0011 | 0.0003 | 0.0013 | 0.0003 | 0.0007 | 0.0011 | 0.0005 | 0.001 | 0.0026 | 0.0002 | 0.0002 | 0.0038 | 0.0001 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.0328 | 10.7507 | 7.3127 | 16.792 | 10.395 | 20.0326 | 12.4552 | 14.6731 | 21.0628 | 14.0129 |
Translational Efficiency | 0.4954 | 0.7481 | 1.0238 | 0.5151 | 0.8085 | 0.5655 | 0.5633 | 0.702 | 0.6593 | 0.667 |
Dataset | Hit |
---|---|
Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
99 | 1307 | 2575 | 888 | 1936 | 2301 | 361 | 973 | 2035 | 3608 | 2936 | 1861 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 749.99 | 777.19 | 865.66 | 886.01 | 701.79 | 762.33 | 873.38 | 937.30 | 704.13 | 767.71 | 866.61 | 912.83 |
Standard Deviation | 144.59 | 99.05 | 114.21 | 132.75 | 86.95 | 99.54 | 157.09 | 132.31 | 91.20 | 99.62 | 120.33 | 134.97 |
Intensity Change Log 2 | — | 0.051396 | 0.206929 | 0.240452 | — | 0.119376 | 0.315570 | 0.417471 | — | 0.084658 | 0.260468 | 0.328737 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001393 | 0.000863 | 0.000295 | 0.001172 | 0.000066 | 0.000310 | 0.000341 | 0.000849 | 0.000131 | 0.000510 | 0.000301 | 0.001003 |
Bud Neck | 0.034014 | 0.024591 | 0.017818 | 0.009216 | 0.003658 | 0.019176 | 0.001987 | 0.014931 | 0.005134 | 0.021137 | 0.015871 | 0.012204 |
Bud Site | 0.052268 | 0.012578 | 0.005846 | 0.026438 | 0.001651 | 0.012603 | 0.000734 | 0.055631 | 0.004113 | 0.012594 | 0.005218 | 0.041701 |
Cell Periphery | 0.000830 | 0.000182 | 0.000128 | 0.000255 | 0.000131 | 0.000189 | 0.001141 | 0.000199 | 0.000165 | 0.000186 | 0.000252 | 0.000226 |
Cytoplasm | 0.118959* | 0.329983* | 0.078021 | 0.253100* | 0.229292* | 0.395165* | 0.118028* | 0.363225* | 0.223924* | 0.371553* | 0.082940 | 0.310677* |
Cytoplasmic Foci | 0.042083 | 0.130596 | 0.000683 | 0.012252 | 0.033616 | 0.084057 | 0.021328 | 0.024559 | 0.034028 | 0.100916 | 0.003221 | 0.018686 |
Eisosomes | 0.000138 | 0.000112 | 0.000015 | 0.000059 | 0.000033 | 0.000055 | 0.000468 | 0.000040 | 0.000038 | 0.000076 | 0.000071 | 0.000049 |
Endoplasmic Reticulum | 0.017170 | 0.002767 | 0.003472 | 0.008798 | 0.001181 | 0.001255 | 0.002816 | 0.002960 | 0.001959 | 0.001803 | 0.003391 | 0.005746 |
Endosome | 0.026779 | 0.009106 | 0.000545 | 0.008502 | 0.001443 | 0.008710 | 0.001099 | 0.019689 | 0.002675 | 0.008853 | 0.000613 | 0.014351 |
Golgi | 0.005814 | 0.004933 | 0.000559 | 0.008386 | 0.000171 | 0.002320 | 0.000138 | 0.008859 | 0.000445 | 0.003266 | 0.000508 | 0.008634 |
Lipid Particles | 0.006199 | 0.006831 | 0.000227 | 0.001736 | 0.002768 | 0.005005 | 0.003461 | 0.001472 | 0.002935 | 0.005667 | 0.000624 | 0.001598 |
Mitochondria | 0.006779 | 0.008875 | 0.001828 | 0.012079 | 0.003102 | 0.010945 | 0.000688 | 0.005178 | 0.003281 | 0.010195 | 0.001688 | 0.008471 |
Mitotic Spindle | 0.010328 | 0.009227 | 0.003961 | 0.055456 | 0.002370 | 0.018241 | 0.000115 | 0.117492* | 0.002757 | 0.014976 | 0.003488 | 0.087891 |
None | 0.012204 | 0.005734 | 0.004365 | 0.014224 | 0.009285 | 0.005882 | 0.041596 | 0.012576 | 0.009427 | 0.005829 | 0.008942 | 0.013363 |
Nuclear Periphery | 0.003511 | 0.001689 | 0.001855 | 0.003359 | 0.002037 | 0.001477 | 0.000589 | 0.001471 | 0.002108 | 0.001553 | 0.001699 | 0.002372 |
Nuclear Periphery Foci | 0.015936 | 0.001443 | 0.000244 | 0.003204 | 0.000451 | 0.000486 | 0.000351 | 0.002559 | 0.001204 | 0.000833 | 0.000257 | 0.002867 |
Nucleolus | 0.026143 | 0.004685 | 0.001483 | 0.001469 | 0.004217 | 0.002511 | 0.004599 | 0.000914 | 0.005284 | 0.003299 | 0.001866 | 0.001179 |
Nucleus | 0.551704* | 0.395839* | 0.871521* | 0.454596* | 0.696801* | 0.389938* | 0.783191* | 0.250628* | 0.689742* | 0.392076* | 0.860660* | 0.347954* |
Peroxisomes | 0.008852 | 0.013219 | 0.000069 | 0.000488 | 0.001208 | 0.006683 | 0.000693 | 0.008105 | 0.001580 | 0.009051 | 0.000146 | 0.004471 |
Vacuole | 0.052681 | 0.035215 | 0.006576 | 0.117735 | 0.006248 | 0.033141 | 0.016460 | 0.104439 | 0.008507 | 0.033892 | 0.007792 | 0.110783 |
Vacuole Periphery | 0.006215 | 0.001533 | 0.000491 | 0.007478 | 0.000273 | 0.001851 | 0.000175 | 0.004222 | 0.000562 | 0.001735 | 0.000452 | 0.005776 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.55 | 3.66 | 1.14 | -0.80 | -5.59 | -4.48 | -6.92 | -16.54 | -7.76 | -0.15 | -4.28 | -10.22 | -12.34 | -4.17 | -7.89 |
Bud Neck | 1.08 | 1.78 | 2.92 | 6.14 | 5.42 | -12.53 | 0.68 | -8.11 | 3.29 | -8.48 | -14.00 | -10.81 | -8.27 | 6.65 | 3.83 |
Bud Site | 3.32 | 3.95 | 2.75 | -2.13 | -4.98 | -7.89 | 0.04 | -10.11 | -5.57 | -10.32 | -6.85 | -0.85 | -9.93 | -5.52 | -9.74 |
Cell Periphery | 2.77 | 3.07 | 2.78 | 0.10 | -4.17 | -2.59 | -5.32 | -1.09 | 2.11 | 5.23 | -1.06 | -1.85 | 0.12 | 1.64 | 2.30 |
Cytoplasm | -9.02 | 2.46 | -3.14 | 12.30 | -15.10 | -19.85 | 13.15 | -2.22 | 14.17 | -13.38 | -19.81 | 24.48 | 0.06 | 19.79 | -24.27 |
Cytoplasmic Foci | -9.58 | 5.47 | 4.33 | 24.31 | -10.01 | -14.17 | 5.17 | 6.10 | 19.57 | -0.80 | -20.36 | 16.83 | 9.82 | 31.42 | -11.16 |
Eisosomes | 1.44 | 7.28 | 3.27 | 3.04 | -18.32 | -6.37 | -3.81 | -12.16 | -5.78 | 3.34 | -8.70 | -1.95 | -13.09 | -0.97 | -1.62 |
Endoplasmic Reticulum | 3.83 | 3.42 | 1.84 | -12.69 | -10.26 | -0.44 | -7.37 | -13.34 | -13.91 | 2.38 | 0.42 | -9.28 | -14.63 | -18.11 | -7.96 |
Endosome | 2.56 | 3.94 | 3.14 | 3.90 | -8.36 | -9.56 | 0.35 | -5.86 | 0.86 | -5.87 | -9.46 | 5.46 | -5.49 | 2.82 | -9.83 |
Golgi | 0.31 | 2.03 | 1.27 | 1.89 | -1.62 | -5.01 | 0.70 | -2.38 | 0.68 | -2.43 | -5.94 | 0.28 | -2.44 | 1.70 | -2.39 |
Lipid Particles | -0.74 | 6.09 | 4.17 | 10.76 | -17.49 | -4.29 | -0.16 | 1.50 | 5.29 | 0.44 | -6.89 | 9.41 | 3.34 | 9.96 | -9.26 |
Mitochondria | -1.44 | 3.54 | 1.40 | 3.06 | -1.42 | -5.42 | 2.71 | 1.59 | 7.65 | -3.07 | -6.06 | 1.83 | 0.50 | 7.06 | -1.40 |
Mitotic Spindle | 0.15 | 0.82 | -5.72 | -7.58 | -8.40 | -7.27 | 2.27 | -14.17 | -11.77 | -14.47 | -7.44 | -1.88 | -16.41 | -13.58 | -16.01 |
None | 2.35 | 2.95 | 1.35 | -1.71 | -2.98 | 3.89 | -3.68 | -0.32 | -3.34 | 3.59 | 4.70 | 1.92 | 0.50 | -3.45 | -1.27 |
Nuclear Periphery | 0.76 | 0.37 | -0.63 | -8.77 | -8.36 | 1.70 | 2.96 | -4.94 | -11.76 | -14.14 | 1.62 | -0.79 | -6.13 | -14.68 | -10.94 |
Nuclear Periphery Foci | 1.71 | 1.90 | 1.13 | -5.18 | -7.55 | -0.30 | -2.22 | -8.56 | -8.54 | -8.13 | 0.57 | 2.06 | -8.52 | -10.23 | -11.43 |
Nucleolus | 2.98 | 3.39 | 3.36 | 5.01 | -0.56 | 4.11 | 0.14 | 2.64 | -1.31 | 1.70 | 3.73 | 5.66 | 5.00 | 2.33 | -1.19 |
Nucleus | 4.23 | -8.74 | 2.44 | -4.54 | 34.38 | 32.25 | -6.38 | 37.63 | 10.74 | 32.12 | 34.38 | -22.49 | 32.11 | 3.25 | 60.14 |
Peroxisomes | -1.79 | 3.93 | 3.73 | 10.88 | -7.73 | -8.12 | 1.88 | -2.21 | 3.10 | -2.84 | -11.81 | 6.84 | -0.65 | 9.35 | -3.85 |
Vacuole | 1.29 | 4.07 | -11.17 | -19.00 | -23.07 | -15.27 | -5.08 | -27.30 | -23.87 | -19.67 | -17.12 | -3.55 | -35.30 | -30.50 | -34.41 |
Vacuole Periphery | 2.66 | 3.34 | 2.25 | -0.68 | -2.40 | -6.04 | 1.28 | -1.96 | 2.59 | -2.24 | -5.89 | 1.00 | -2.37 | 0.93 | -2.69 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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