Standard name | |
---|---|
Human Ortholog | |
Description | Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 3 |
Bud | 7 | 4 | 1 | 2 | 8 | 9 | 24 | 23 | 29 | 24 | 9 | 20 | 5 | 4 | 0 | 2 | 3 | 0 | 8 | 2 | 0 | 3 | 13 | 24 |
Bud Neck | 4 | 3 | 1 | 2 | 5 | 7 | 9 | 8 | 3 | 13 | 6 | 9 | 0 | 3 | 1 | 1 | 4 | 0 | 4 | 4 | 0 | 0 | 5 | 13 |
Bud Site | 7 | 11 | 4 | 7 | 6 | 22 | 20 | 22 | 27 | 29 | 19 | 29 | 1 | 3 | 0 | 19 | 8 | 8 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 18 | 5 | 4 | 7 | 12 | 10 | 14 | 9 | 7 | 6 | 6 | 6 | 11 | 31 | 35 | 12 | 19 | 9 | 0 | 0 | 0 | 0 | 1 | 6 |
Endoplasmic Reticulum | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 3 | 2 | 1 | 2 | 4 | 1 | 2 | 3 | 0 | 1 | 0 | 0 | 7 | 9 | 9 | 11 | 13 | 13 | 6 | 4 | 4 | 0 | 4 | 9 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 0 |
Mitochondria | 14 | 5 | 0 | 1 | 2 | 5 | 14 | 10 | 19 | 13 | 14 | 16 | 0 | 0 | 1 | 17 | 6 | 9 | 2 | 2 | 0 | 0 | 4 | 4 |
Nucleus | 4 | 1 | 2 | 2 | 2 | 3 | 5 | 6 | 10 | 17 | 8 | 5 | 2 | 4 | 2 | 3 | 6 | 5 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 28 | 20 | 10 | 15 | 12 | 15 | 28 | 23 | 42 | 43 | 42 | 43 | 14 | 16 | 13 | 16 | 22 | 12 | 4 | 3 | 0 | 1 | 5 | 4 |
Peroxisomes | 34 | 15 | 2 | 2 | 8 | 24 | 13 | 9 | 11 | 10 | 8 | 11 | 14 | 24 | 49 | 24 | 22 | 23 | 4 | 3 | 2 | 2 | 5 | 6 |
SpindlePole | 174 | 172 | 77 | 131 | 258 | 167 | 227 | 248 | 205 | 274 | 133 | 220 | 163 | 250 | 210 | 148 | 163 | 94 | 190 | 174 | 99 | 112 | 273 | 263 |
Vac/Vac Membrane | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 4 | 0 | 3 | 2 | 1 | 0 | 0 | 0 | 1 | 3 | 2 |
Unique Cell Count | 218 | 195 | 87 | 145 | 280 | 189 | 259 | 284 | 249 | 324 | 169 | 261 | 187 | 304 | 267 | 176 | 205 | 132 | 226 | 203 | 111 | 128 | 323 | 347 |
Labelled Cell Count | 294 | 240 | 102 | 174 | 319 | 264 | 356 | 362 | 355 | 432 | 245 | 361 | 218 | 348 | 320 | 257 | 271 | 175 | 226 | 203 | 111 | 128 | 323 | 347 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.3 | 6.2 | 5.9 | 5.5 | 6.0 | 4.9 | 5.0 | 5.5 | 4.9 | 5.2 | 4.9 | 5.0 | 6.3 | 6.8 | 6.4 | 7.4 | 8.7 | 8.2 | 5.6 | 6.3 | 6.6 |
Std Deviation (1e-4) | 1.0 | 1.6 | 1.6 | 1.6 | 1.6 | 1.5 | 1.7 | 1.8 | 1.4 | 1.8 | 1.6 | 1.3 | 1.3 | 1.5 | 1.4 | 1.9 | 2.6 | 2.5 | 1.6 | 1.8 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.11 | 0.02 | -0.27 | -0.24 | -0.09 | -0.28 | -0.17 | -0.26 | -0.24 | 0.09 | 0.21 | 0.13 | 0.32 | 0.56 | 0.47 | -0.07 | 0.1 | 0.15 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | 0 | 0 | 0 | 2.5 | 2.3 | – | 2.2 | 0 | 2.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 1.9 | 1.0 | 1.0 | – | 1.3 | 1.8 | 1.8 | 0 | 0 | 0 | 1.7 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.6 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | -0.3 | -2.5 | -1.0 | -0.2 | -1.0 | – | 0.4 | 2.5 | 1.1 | -1.1 | -2.1 | -2.2 | -0.6 | -0.2 | -0.6 |
Peroxisomes | 0 | 0 | 2.7 | 0 | 0 | – | 0 | 0 | 0 | 1.7 | 1.8 | 3.7 | 2.9 | 2.4 | 3.4 |
SpindlePole | 0.4 | 1.1 | 0 | -0.2 | -0.3 | – | -0.9 | -1.9 | -1.0 | -0.3 | -1.4 | -2.0 | -1.0 | -1.8 | -3.0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1.6 | 2.2 | 0.7 | 1.4 | 0.7 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.0755 | 2.7752 | 3.2414 | 3.1619 | 2.3333 | 3.0861 | 3.598 | 3.1077 | 3.2404 | 2.6638 | 2.6712 | 2.7066 | 2.5456 | 1.4015 | 1.5368 | 1.7616 | 1.5697 | 1.4544 |
Actin | 0.0161 | 0.0001 | 0.0188 | 0.0016 | 0.0331 | 0.0129 | 0.0352 | 0.0009 | 0.0123 | 0.0616 | 0.0001 | 0.0109 | 0.0205 | 0.0037 | 0.0231 | 0.0237 | 0.0075 | 0.0052 |
Bud | 0.0002 | 0.0003 | 0.0004 | 0.0001 | 0.0004 | 0.0002 | 0.0005 | 0.0002 | 0.0008 | 0.0003 | 0.0001 | 0 | 0.0004 | 0.0027 | 0.0002 | 0.0005 | 0.0002 | 0.0003 |
Bud Neck | 0.001 | 0.0031 | 0.0009 | 0.0004 | 0.0003 | 0.0002 | 0.0014 | 0.0006 | 0.001 | 0.0018 | 0.0016 | 0.0002 | 0.0016 | 0.0015 | 0.0005 | 0.0011 | 0.0004 | 0.0005 |
Bud Periphery | 0.0004 | 0.0003 | 0.0004 | 0.0002 | 0.0009 | 0.0003 | 0.0011 | 0.0002 | 0.0007 | 0.0005 | 0.0001 | 0 | 0.0006 | 0.0032 | 0.0004 | 0.0006 | 0.0001 | 0.0004 |
Bud Site | 0.0013 | 0.0117 | 0.0053 | 0.0013 | 0.0008 | 0.0004 | 0.0041 | 0.0024 | 0.006 | 0.0018 | 0.0009 | 0.0001 | 0.0045 | 0.0219 | 0.0016 | 0.0021 | 0.0009 | 0.0003 |
Cell Periphery | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 |
Cytoplasm | 0.0003 | 0.0001 | 0.0004 | 0.0003 | 0.0002 | 0.0001 | 0.0012 | 0.0021 | 0.0023 | 0.0026 | 0 | 0.0001 | 0.0011 | 0.0004 | 0.0002 | 0.0057 | 0.0002 | 0.0008 |
Cytoplasmic Foci | 0.1867 | 0.128 | 0.1067 | 0.1385 | 0.184 | 0.0341 | 0.1996 | 0.1335 | 0.0967 | 0.1181 | 0.1972 | 0.0382 | 0.1862 | 0.1074 | 0.097 | 0.12 | 0.2768 | 0.05 |
Eisosomes | 0.0001 | 0 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0005 | 0 | 0.0001 | 0.0003 | 0 | 0 | 0.0002 | 0 | 0.0002 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0003 | 0.0001 | 0.0009 | 0.0004 | 0.0015 | 0.0001 | 0.0012 | 0.0015 | 0.0002 | 0.0031 | 0 | 0.0001 | 0.0009 | 0.0005 | 0.0006 | 0.0012 | 0.0001 | 0.0006 |
Endosome | 0.0112 | 0.0008 | 0.0061 | 0.014 | 0.0206 | 0.0011 | 0.0155 | 0.0032 | 0.0026 | 0.0238 | 0.0011 | 0.0009 | 0.0153 | 0.0035 | 0.0077 | 0.0123 | 0.0026 | 0.0034 |
Golgi | 0.0098 | 0.0008 | 0.0024 | 0.0077 | 0.0134 | 0.0018 | 0.0098 | 0.0019 | 0.0046 | 0.0135 | 0.0032 | 0.0025 | 0.012 | 0.0012 | 0.0063 | 0.0055 | 0.0073 | 0.0024 |
Lipid Particles | 0.0778 | 0.0186 | 0.0325 | 0.0291 | 0.0663 | 0.0136 | 0.0869 | 0.0215 | 0.0299 | 0.0454 | 0.1338 | 0.0104 | 0.0914 | 0.0162 | 0.0294 | 0.0202 | 0.1172 | 0.0242 |
Mitochondria | 0.0023 | 0.0001 | 0.0048 | 0.0061 | 0.0191 | 0.0049 | 0.0081 | 0.0059 | 0.0066 | 0.0066 | 0.002 | 0.0004 | 0.0074 | 0.0004 | 0.0112 | 0.006 | 0.0017 | 0.0012 |
None | 0.0003 | 0.0002 | 0.0007 | 0.0005 | 0.0004 | 0.0002 | 0.0023 | 0.0002 | 0.0018 | 0.001 | 0 | 0.0002 | 0.0012 | 0.0005 | 0.0007 | 0.0033 | 0.0003 | 0.0015 |
Nuclear Periphery | 0.0005 | 0.0145 | 0.0071 | 0.0014 | 0.0098 | 0.0003 | 0.0034 | 0.0084 | 0.0006 | 0.0038 | 0.0004 | 0.0005 | 0.0056 | 0.0038 | 0.0006 | 0.009 | 0.0002 | 0.0033 |
Nucleolus | 0.0513 | 0.1029 | 0.0287 | 0.0076 | 0.0096 | 0.0047 | 0.0486 | 0.0668 | 0.0276 | 0.0022 | 0.0922 | 0.0172 | 0.0378 | 0.0882 | 0.0398 | 0.0024 | 0.0089 | 0.0155 |
Nucleus | 0.0041 | 0.0121 | 0.0048 | 0.0007 | 0.0008 | 0.0005 | 0.0027 | 0.0044 | 0.0025 | 0.0008 | 0.0019 | 0.0012 | 0.0028 | 0.0082 | 0.0026 | 0.0034 | 0.0003 | 0.0017 |
Peroxisomes | 0.0133 | 0.0043 | 0.0078 | 0.0061 | 0.0217 | 0.0046 | 0.0203 | 0.0088 | 0.0089 | 0.0211 | 0.0205 | 0.0066 | 0.0189 | 0.0035 | 0.0063 | 0.0035 | 0.0399 | 0.008 |
Punctate Nuclear | 0.6216 | 0.7012 | 0.7702 | 0.7812 | 0.6053 | 0.9199 | 0.5551 | 0.7365 | 0.7928 | 0.6895 | 0.5446 | 0.9102 | 0.5893 | 0.7317 | 0.7708 | 0.7734 | 0.5353 | 0.8801 |
Vacuole | 0.001 | 0.0004 | 0.0003 | 0.0018 | 0.0031 | 0 | 0.0012 | 0.0004 | 0.0014 | 0.0011 | 0.0001 | 0 | 0.0012 | 0.0007 | 0.0003 | 0.0041 | 0.0001 | 0.0003 |
Vacuole Periphery | 0.0004 | 0.0003 | 0.0005 | 0.0009 | 0.0084 | 0 | 0.001 | 0.0006 | 0.0004 | 0.0009 | 0 | 0 | 0.0009 | 0.0006 | 0.0003 | 0.0016 | 0 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 18.188 | 9.0327 | 10.054 | 10.7574 | 21.6164 | 15.1483 | 10.2746 | 9.2462 | 8.5268 | 24.389 |
Translational Efficiency | 2.8964 | 2.3706 | 1.0283 | 1.4917 | 1.8058 | 3.6482 | 1.9695 | 1.7867 | 2.1628 | 1.5681 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
36 | 479 | 2451 | 1530 | 1564 | 2009 | 2057 | 1106 | 1600 | 2488 | 4508 | 2636 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1028.77 | 828.84 | 946.20 | 949.91 | 735.25 | 797.81 | 957.98 | 988.18 | 741.85 | 803.78 | 951.58 | 965.97 |
Standard Deviation | 334.32 | 127.65 | 138.48 | 166.19 | 107.59 | 112.28 | 133.94 | 154.90 | 125.40 | 116.04 | 136.55 | 162.65 |
Intensity Change Log 2 | — | -0.311755 | -0.120703 | -0.115058 | — | 0.117810 | 0.381761 | 0.426539 | — | -0.116963 | 0.110303 | 0.135769 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002515 | 0.000500 | 0.000112 | 0.001178 | 0.000549 | 0.000629 | 0.000221 | 0.000859 | 0.000593 | 0.000604 | 0.000162 | 0.001044 |
Bud Neck | 0.012619 | 0.006993 | 0.002793 | 0.006290 | 0.007658 | 0.017797 | 0.006629 | 0.004873 | 0.007769 | 0.015717 | 0.004543 | 0.005695 |
Bud Site | 0.046312 | 0.018601 | 0.006775 | 0.025509 | 0.014595 | 0.024905 | 0.014378 | 0.041500 | 0.015309 | 0.023691 | 0.010244 | 0.032218 |
Cell Periphery | 0.000346 | 0.000150 | 0.000047 | 0.000216 | 0.000305 | 0.000442 | 0.000100 | 0.000087 | 0.000305 | 0.000386 | 0.000071 | 0.000162 |
Cytoplasm | 0.043813 | 0.009776 | 0.004328 | 0.012291 | 0.020591 | 0.039805 | 0.008485 | 0.014345 | 0.021114 | 0.034024 | 0.006225 | 0.013153 |
Cytoplasmic Foci | 0.093841 | 0.152124 | 0.039805 | 0.095087 | 0.184726* | 0.200751* | 0.071476 | 0.126929 | 0.182681* | 0.191389* | 0.054256 | 0.108447 |
Eisosomes | 0.000078 | 0.000210 | 0.000047 | 0.000076 | 0.000336 | 0.000151 | 0.000085 | 0.000049 | 0.000330 | 0.000162 | 0.000064 | 0.000064 |
Endoplasmic Reticulum | 0.004950 | 0.000828 | 0.000189 | 0.000319 | 0.001287 | 0.001161 | 0.000423 | 0.000223 | 0.001369 | 0.001097 | 0.000296 | 0.000279 |
Endosome | 0.033920 | 0.011564 | 0.001959 | 0.005143 | 0.010589 | 0.022829 | 0.004071 | 0.005823 | 0.011114 | 0.020660 | 0.002923 | 0.005428 |
Golgi | 0.012639 | 0.011409 | 0.001015 | 0.003877 | 0.011390 | 0.012610 | 0.001925 | 0.005800 | 0.011418 | 0.012379 | 0.001430 | 0.004684 |
Lipid Particles | 0.015479 | 0.019881 | 0.002369 | 0.006291 | 0.022987 | 0.024876 | 0.004206 | 0.002888 | 0.022818 | 0.023915 | 0.003207 | 0.004864 |
Mitochondria | 0.002383 | 0.002941 | 0.000299 | 0.002207 | 0.002357 | 0.007159 | 0.000408 | 0.001264 | 0.002358 | 0.006347 | 0.000349 | 0.001811 |
Mitotic Spindle | 0.510677* | 0.607137* | 0.901820* | 0.779089* | 0.513814* | 0.460830* | 0.816043* | 0.734407* | 0.513744* | 0.488998* | 0.862680* | 0.760342* |
None | 0.001633 | 0.004369 | 0.000341 | 0.001557 | 0.004792 | 0.006132 | 0.000882 | 0.000248 | 0.004720 | 0.005793 | 0.000588 | 0.001007 |
Nuclear Periphery | 0.002666 | 0.000395 | 0.000312 | 0.000423 | 0.000760 | 0.000875 | 0.000439 | 0.000313 | 0.000803 | 0.000782 | 0.000370 | 0.000377 |
Nuclear Periphery Foci | 0.071005 | 0.036666 | 0.006183 | 0.005484 | 0.065838 | 0.051386 | 0.017302 | 0.004418 | 0.065954 | 0.048552 | 0.011257 | 0.005037 |
Nucleolus | 0.028290 | 0.021980 | 0.005761 | 0.005629 | 0.036126 | 0.035106 | 0.011999 | 0.003058 | 0.035950 | 0.032579 | 0.008607 | 0.004550 |
Nucleus | 0.041452 | 0.007946 | 0.003345 | 0.003198 | 0.012645 | 0.016198 | 0.006616 | 0.001737 | 0.013293 | 0.014609 | 0.004837 | 0.002585 |
Peroxisomes | 0.031110 | 0.082912 | 0.021484 | 0.039029 | 0.084489 | 0.070666 | 0.032284 | 0.042543 | 0.083288 | 0.073024 | 0.026412 | 0.040503 |
Vacuole | 0.035518 | 0.003271 | 0.000800 | 0.005342 | 0.003851 | 0.004729 | 0.001844 | 0.005636 | 0.004563 | 0.004448 | 0.001277 | 0.005465 |
Vacuole Periphery | 0.008755 | 0.000344 | 0.000216 | 0.001767 | 0.000317 | 0.000963 | 0.000182 | 0.003002 | 0.000507 | 0.000844 | 0.000200 | 0.002285 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 2.00 | 2.33 | 1.25 | -1.17 | -1.82 | -0.87 | 1.45 | -2.58 | -1.86 | -4.16 | -0.49 | 2.59 | -1.64 | -1.46 | -2.58 |
Bud Neck | 1.92 | 2.67 | 1.68 | -0.72 | -3.63 | -6.69 | -1.48 | -0.48 | 6.03 | 0.92 | -6.04 | 1.39 | -0.85 | 5.21 | -2.24 |
Bud Site | 1.77 | 2.34 | 0.96 | -3.60 | -9.07 | -5.65 | -3.14 | -9.54 | -6.88 | -7.98 | -4.91 | 0.45 | -11.23 | -7.32 | -11.72 |
Cell Periphery | 1.28 | 1.89 | 0.80 | -0.73 | -1.91 | -2.40 | 6.00 | 6.31 | 7.66 | 0.74 | -1.52 | 7.60 | 2.07 | 3.06 | -1.76 |
Cytoplasm | 1.30 | 1.42 | 1.02 | -3.05 | -4.92 | -4.98 | 3.21 | -0.74 | 3.59 | -3.18 | -3.73 | 5.05 | 0.03 | 3.97 | -5.62 |
Cytoplasmic Foci | -1.66 | 1.93 | -0.18 | 3.85 | -10.28 | -1.99 | 13.53 | 4.52 | 6.57 | -7.17 | -1.29 | 17.58 | 7.68 | 10.28 | -12.04 |
Eisosomes | -3.35 | 1.84 | 0.59 | 3.89 | -2.81 | 8.03 | 11.15 | 13.71 | 15.78 | 6.78 | 7.08 | 12.58 | 12.95 | 11.30 | 1.72 |
Endoplasmic Reticulum | 2.41 | 2.82 | 2.75 | 5.06 | -2.29 | 1.76 | 7.45 | 10.36 | 11.27 | 4.67 | 2.79 | 9.82 | 10.22 | 11.71 | 1.35 |
Endosome | 1.85 | 2.64 | 2.39 | 3.92 | -3.86 | -6.16 | 5.91 | 4.51 | 9.39 | -0.71 | -5.36 | 8.52 | 5.76 | 10.61 | -3.17 |
Golgi | 0.09 | 2.37 | 1.85 | 5.55 | -3.24 | 1.58 | 14.45 | 6.40 | 5.57 | -1.78 | 1.80 | 15.60 | 9.86 | 9.48 | -3.32 |
Lipid Particles | -0.33 | 1.50 | 1.07 | 3.92 | -3.61 | 0.32 | 9.53 | 10.60 | 11.50 | 2.17 | 0.61 | 10.45 | 9.40 | 10.75 | -2.12 |
Mitochondria | 0.16 | 2.42 | 0.44 | 0.22 | -2.30 | -3.38 | 1.86 | 0.76 | 4.27 | -1.61 | -3.22 | 2.06 | -0.03 | 3.45 | -2.69 |
Mitotic Spindle | -1.37 | -5.48 | -3.61 | -7.73 | 12.74 | 1.83 | -23.16* | -14.14 | -16.74 | 5.94 | 0.04 | -31.32 | -19.45 | -22.42 | 12.81 |
None | -1.01 | 1.32 | 0.09 | 1.17 | -1.86 | -1.02 | 4.17 | 5.16 | 6.54 | 3.05 | -0.77 | 4.75 | 3.91 | 5.21 | -0.98 |
Nuclear Periphery | 2.16 | 2.22 | 2.07 | -1.45 | -2.43 | -1.62 | 2.69 | 5.28 | 7.58 | 3.90 | -0.03 | 4.74 | 4.41 | 5.64 | -0.19 |
Nuclear Periphery Foci | 0.89 | 2.26 | 2.34 | 7.51 | 1.57 | 3.64 | 14.19 | 19.54* | 18.18* | 7.79 | 4.58 | 17.19 | 19.78 | 19.70 | 7.09 |
Nucleolus | 0.73 | 2.10 | 2.09 | 3.00 | -0.01 | 1.02 | 5.99 | 10.51 | 10.95 | 6.48 | 1.67 | 8.29 | 10.21 | 10.74 | 4.46 |
Nucleus | 1.97 | 2.15 | 2.12 | 1.31 | -0.42 | -1.68 | 1.03 | 5.17 | 8.00 | 4.61 | -0.63 | 3.24 | 4.89 | 7.21 | 2.74 |
Peroxisomes | -3.46 | 0.97 | -0.27 | 6.36 | -5.32 | 2.57 | 11.84 | 8.93 | 7.20 | -1.81 | 1.88 | 14.25 | 10.48 | 10.32 | -4.99 |
Vacuole | 2.75 | 2.93 | 2.32 | -3.35 | -5.13 | -0.42 | 1.04 | -3.40 | -3.27 | -3.97 | 0.52 | 3.07 | -3.45 | -4.21 | -6.28 |
Vacuole Periphery | 2.13 | 2.15 | 1.81 | -2.79 | -2.86 | -4.84 | 0.67 | -2.23 | -1.71 | -2.27 | -1.54 | 1.81 | -3.00 | -2.62 | -3.51 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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