Standard name
Human Ortholog
Description Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0.05 0.09 0.08 0.12 0.07 0.05 0.08 0 0 0 0 0 0 0 0 0 0 0 0.07
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0.06 0.05 0.05 0 0.12 0.08 0.08 0.11 0.09 0.11 0.11 0 0 0 0.11 0 0.06
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.08 0 0.05 0.05 0 0.05 0.05 0 0 0 0 0 0.06 0.1 0.13 0.07 0.09 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.1 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0 0 0 0.05 0 0.08 0 0.08 0.06 0 0 0 0.1 0 0.07 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.13 0.1 0.11 0.1 0 0.08 0.11 0.08 0.17 0.13 0.25 0.16 0.07 0.05 0.05 0.09 0.11 0.09 0 0 0 0 0 0
Peroxisomes 0.16 0.08 0 0 0 0.13 0.05 0 0 0 0.05 0 0.07 0.08 0.18 0.14 0.11 0.17 0 0 0 0 0 0
SpindlePole 0.8 0.88 0.89 0.9 0.92 0.88 0.88 0.87 0.82 0.85 0.79 0.84 0.87 0.82 0.79 0.84 0.8 0.71 0.84 0.86 0.9 0.88 0.85 0.76
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 1 1 0 1 2 1 0 0 0 0 0 0 0 0 1 1 0 0 0 3
Bud 7 4 1 2 8 9 24 23 29 24 9 20 5 4 0 2 3 0 8 2 0 3 13 24
Bud Neck 4 3 1 2 5 7 9 8 3 13 6 9 0 3 1 1 4 0 4 4 0 0 5 13
Bud Site 7 11 4 7 6 22 20 22 27 29 19 29 1 3 0 19 8 8
Cell Periphery 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 18 5 4 7 12 10 14 9 7 6 6 6 11 31 35 12 19 9 0 0 0 0 1 6
Endoplasmic Reticulum 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 2 0 0 0 0 0 0 0
Endosome 3 2 1 2 4 1 2 3 0 1 0 0 7 9 9 11 13 13 6 4 4 0 4 9
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 1 1 0
Mitochondria 14 5 0 1 2 5 14 10 19 13 14 16 0 0 1 17 6 9 2 2 0 0 4 4
Nucleus 4 1 2 2 2 3 5 6 10 17 8 5 2 4 2 3 6 5 0 1 0 0 0 0
Nuclear Periphery 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 28 20 10 15 12 15 28 23 42 43 42 43 14 16 13 16 22 12 4 3 0 1 5 4
Peroxisomes 34 15 2 2 8 24 13 9 11 10 8 11 14 24 49 24 22 23 4 3 2 2 5 6
SpindlePole 174 172 77 131 258 167 227 248 205 274 133 220 163 250 210 148 163 94 190 174 99 112 273 263
Vac/Vac Membrane 0 0 0 2 1 0 0 0 0 1 0 1 0 4 0 3 2 1 0 0 0 1 3 2
Unique Cell Count 218 195 87 145 280 189 259 284 249 324 169 261 187 304 267 176 205 132 226 203 111 128 323 347
Labelled Cell Count 294 240 102 174 319 264 356 362 355 432 245 361 218 348 320 257 271 175 226 203 111 128 323 347


SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 6.2 5.9 5.5 6.0 4.9 5.0 5.5 4.9 5.2 4.9 5.0 6.3 6.8 6.4 7.4 8.7 8.2 5.6 6.3 6.6
Std Deviation (1e-4) 1.0 1.6 1.6 1.6 1.6 1.5 1.7 1.8 1.4 1.8 1.6 1.3 1.3 1.5 1.4 1.9 2.6 2.5 1.6 1.8 1.7
Intensity Change (Log2) -0.11 0.02 -0.27 -0.24 -0.09 -0.28 -0.17 -0.26 -0.24 0.09 0.21 0.13 0.32 0.56 0.47 -0.07 0.1 0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0 2.5 2.3 2.2 0 2.2 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 1.9 1.0 1.0 1.3 1.8 1.8 0 0 0 1.7 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 2.6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 3.0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuc
Nucleolus -0.3 -2.5 -1.0 -0.2 -1.0 0.4 2.5 1.1 -1.1 -2.1 -2.2 -0.6 -0.2 -0.6
Peroxisomes 0 0 2.7 0 0 0 0 0 1.7 1.8 3.7 2.9 2.4 3.4
SpindlePole 0.4 1.1 0 -0.2 -0.3 -0.9 -1.9 -1.0 -0.3 -1.4 -2.0 -1.0 -1.8 -3.0
Vac
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 1.6 2.2 0.7 1.4 0.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.0755 2.7752 3.2414 3.1619 2.3333 3.0861 3.598 3.1077 3.2404 2.6638 2.6712 2.7066 2.5456 1.4015 1.5368 1.7616 1.5697 1.4544
Actin 0.0161 0.0001 0.0188 0.0016 0.0331 0.0129 0.0352 0.0009 0.0123 0.0616 0.0001 0.0109 0.0205 0.0037 0.0231 0.0237 0.0075 0.0052
Bud 0.0002 0.0003 0.0004 0.0001 0.0004 0.0002 0.0005 0.0002 0.0008 0.0003 0.0001 0 0.0004 0.0027 0.0002 0.0005 0.0002 0.0003
Bud Neck 0.001 0.0031 0.0009 0.0004 0.0003 0.0002 0.0014 0.0006 0.001 0.0018 0.0016 0.0002 0.0016 0.0015 0.0005 0.0011 0.0004 0.0005
Bud Periphery 0.0004 0.0003 0.0004 0.0002 0.0009 0.0003 0.0011 0.0002 0.0007 0.0005 0.0001 0 0.0006 0.0032 0.0004 0.0006 0.0001 0.0004
Bud Site 0.0013 0.0117 0.0053 0.0013 0.0008 0.0004 0.0041 0.0024 0.006 0.0018 0.0009 0.0001 0.0045 0.0219 0.0016 0.0021 0.0009 0.0003
Cell Periphery 0.0001 0.0001 0 0.0001 0.0001 0 0.0003 0 0.0001 0.0001 0 0 0.0002 0.0002 0.0001 0.0001 0 0
Cytoplasm 0.0003 0.0001 0.0004 0.0003 0.0002 0.0001 0.0012 0.0021 0.0023 0.0026 0 0.0001 0.0011 0.0004 0.0002 0.0057 0.0002 0.0008
Cytoplasmic Foci 0.1867 0.128 0.1067 0.1385 0.184 0.0341 0.1996 0.1335 0.0967 0.1181 0.1972 0.0382 0.1862 0.1074 0.097 0.12 0.2768 0.05
Eisosomes 0.0001 0 0.0002 0 0.0002 0.0001 0.0005 0 0.0001 0.0003 0 0 0.0002 0 0.0002 0.0001 0 0
Endoplasmic Reticulum 0.0003 0.0001 0.0009 0.0004 0.0015 0.0001 0.0012 0.0015 0.0002 0.0031 0 0.0001 0.0009 0.0005 0.0006 0.0012 0.0001 0.0006
Endosome 0.0112 0.0008 0.0061 0.014 0.0206 0.0011 0.0155 0.0032 0.0026 0.0238 0.0011 0.0009 0.0153 0.0035 0.0077 0.0123 0.0026 0.0034
Golgi 0.0098 0.0008 0.0024 0.0077 0.0134 0.0018 0.0098 0.0019 0.0046 0.0135 0.0032 0.0025 0.012 0.0012 0.0063 0.0055 0.0073 0.0024
Lipid Particles 0.0778 0.0186 0.0325 0.0291 0.0663 0.0136 0.0869 0.0215 0.0299 0.0454 0.1338 0.0104 0.0914 0.0162 0.0294 0.0202 0.1172 0.0242
Mitochondria 0.0023 0.0001 0.0048 0.0061 0.0191 0.0049 0.0081 0.0059 0.0066 0.0066 0.002 0.0004 0.0074 0.0004 0.0112 0.006 0.0017 0.0012
None 0.0003 0.0002 0.0007 0.0005 0.0004 0.0002 0.0023 0.0002 0.0018 0.001 0 0.0002 0.0012 0.0005 0.0007 0.0033 0.0003 0.0015
Nuclear Periphery 0.0005 0.0145 0.0071 0.0014 0.0098 0.0003 0.0034 0.0084 0.0006 0.0038 0.0004 0.0005 0.0056 0.0038 0.0006 0.009 0.0002 0.0033
Nucleolus 0.0513 0.1029 0.0287 0.0076 0.0096 0.0047 0.0486 0.0668 0.0276 0.0022 0.0922 0.0172 0.0378 0.0882 0.0398 0.0024 0.0089 0.0155
Nucleus 0.0041 0.0121 0.0048 0.0007 0.0008 0.0005 0.0027 0.0044 0.0025 0.0008 0.0019 0.0012 0.0028 0.0082 0.0026 0.0034 0.0003 0.0017
Peroxisomes 0.0133 0.0043 0.0078 0.0061 0.0217 0.0046 0.0203 0.0088 0.0089 0.0211 0.0205 0.0066 0.0189 0.0035 0.0063 0.0035 0.0399 0.008
Punctate Nuclear 0.6216 0.7012 0.7702 0.7812 0.6053 0.9199 0.5551 0.7365 0.7928 0.6895 0.5446 0.9102 0.5893 0.7317 0.7708 0.7734 0.5353 0.8801
Vacuole 0.001 0.0004 0.0003 0.0018 0.0031 0 0.0012 0.0004 0.0014 0.0011 0.0001 0 0.0012 0.0007 0.0003 0.0041 0.0001 0.0003
Vacuole Periphery 0.0004 0.0003 0.0005 0.0009 0.0084 0 0.001 0.0006 0.0004 0.0009 0 0 0.0009 0.0006 0.0003 0.0016 0 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.188 9.0327 10.054 10.7574 21.6164 15.1483 10.2746 9.2462 8.5268 24.389
Translational Efficiency 2.8964 2.3706 1.0283 1.4917 1.8058 3.6482 1.9695 1.7867 2.1628 1.5681

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
36 479 2451 1530 1564 2009 2057 1106 1600 2488 4508 2636

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1028.77 828.84 946.20 949.91 735.25 797.81 957.98 988.18 741.85 803.78 951.58 965.97
Standard Deviation 334.32 127.65 138.48 166.19 107.59 112.28 133.94 154.90 125.40 116.04 136.55 162.65
Intensity Change Log 2 -0.311755 -0.120703 -0.115058 0.117810 0.381761 0.426539 -0.116963 0.110303 0.135769

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002515 0.000500 0.000112 0.001178 0.000549 0.000629 0.000221 0.000859 0.000593 0.000604 0.000162 0.001044
Bud Neck 0.012619 0.006993 0.002793 0.006290 0.007658 0.017797 0.006629 0.004873 0.007769 0.015717 0.004543 0.005695
Bud Site 0.046312 0.018601 0.006775 0.025509 0.014595 0.024905 0.014378 0.041500 0.015309 0.023691 0.010244 0.032218
Cell Periphery 0.000346 0.000150 0.000047 0.000216 0.000305 0.000442 0.000100 0.000087 0.000305 0.000386 0.000071 0.000162
Cytoplasm 0.043813 0.009776 0.004328 0.012291 0.020591 0.039805 0.008485 0.014345 0.021114 0.034024 0.006225 0.013153
Cytoplasmic Foci 0.093841 0.152124 0.039805 0.095087 0.184726* 0.200751* 0.071476 0.126929 0.182681* 0.191389* 0.054256 0.108447
Eisosomes 0.000078 0.000210 0.000047 0.000076 0.000336 0.000151 0.000085 0.000049 0.000330 0.000162 0.000064 0.000064
Endoplasmic Reticulum 0.004950 0.000828 0.000189 0.000319 0.001287 0.001161 0.000423 0.000223 0.001369 0.001097 0.000296 0.000279
Endosome 0.033920 0.011564 0.001959 0.005143 0.010589 0.022829 0.004071 0.005823 0.011114 0.020660 0.002923 0.005428
Golgi 0.012639 0.011409 0.001015 0.003877 0.011390 0.012610 0.001925 0.005800 0.011418 0.012379 0.001430 0.004684
Lipid Particles 0.015479 0.019881 0.002369 0.006291 0.022987 0.024876 0.004206 0.002888 0.022818 0.023915 0.003207 0.004864
Mitochondria 0.002383 0.002941 0.000299 0.002207 0.002357 0.007159 0.000408 0.001264 0.002358 0.006347 0.000349 0.001811
Mitotic Spindle 0.510677* 0.607137* 0.901820* 0.779089* 0.513814* 0.460830* 0.816043* 0.734407* 0.513744* 0.488998* 0.862680* 0.760342*
None 0.001633 0.004369 0.000341 0.001557 0.004792 0.006132 0.000882 0.000248 0.004720 0.005793 0.000588 0.001007
Nuclear Periphery 0.002666 0.000395 0.000312 0.000423 0.000760 0.000875 0.000439 0.000313 0.000803 0.000782 0.000370 0.000377
Nuclear Periphery Foci 0.071005 0.036666 0.006183 0.005484 0.065838 0.051386 0.017302 0.004418 0.065954 0.048552 0.011257 0.005037
Nucleolus 0.028290 0.021980 0.005761 0.005629 0.036126 0.035106 0.011999 0.003058 0.035950 0.032579 0.008607 0.004550
Nucleus 0.041452 0.007946 0.003345 0.003198 0.012645 0.016198 0.006616 0.001737 0.013293 0.014609 0.004837 0.002585
Peroxisomes 0.031110 0.082912 0.021484 0.039029 0.084489 0.070666 0.032284 0.042543 0.083288 0.073024 0.026412 0.040503
Vacuole 0.035518 0.003271 0.000800 0.005342 0.003851 0.004729 0.001844 0.005636 0.004563 0.004448 0.001277 0.005465
Vacuole Periphery 0.008755 0.000344 0.000216 0.001767 0.000317 0.000963 0.000182 0.003002 0.000507 0.000844 0.000200 0.002285

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.00 2.33 1.25 -1.17 -1.82 -0.87 1.45 -2.58 -1.86 -4.16 -0.49 2.59 -1.64 -1.46 -2.58
Bud Neck 1.92 2.67 1.68 -0.72 -3.63 -6.69 -1.48 -0.48 6.03 0.92 -6.04 1.39 -0.85 5.21 -2.24
Bud Site 1.77 2.34 0.96 -3.60 -9.07 -5.65 -3.14 -9.54 -6.88 -7.98 -4.91 0.45 -11.23 -7.32 -11.72
Cell Periphery 1.28 1.89 0.80 -0.73 -1.91 -2.40 6.00 6.31 7.66 0.74 -1.52 7.60 2.07 3.06 -1.76
Cytoplasm 1.30 1.42 1.02 -3.05 -4.92 -4.98 3.21 -0.74 3.59 -3.18 -3.73 5.05 0.03 3.97 -5.62
Cytoplasmic Foci -1.66 1.93 -0.18 3.85 -10.28 -1.99 13.53 4.52 6.57 -7.17 -1.29 17.58 7.68 10.28 -12.04
Eisosomes -3.35 1.84 0.59 3.89 -2.81 8.03 11.15 13.71 15.78 6.78 7.08 12.58 12.95 11.30 1.72
Endoplasmic Reticulum 2.41 2.82 2.75 5.06 -2.29 1.76 7.45 10.36 11.27 4.67 2.79 9.82 10.22 11.71 1.35
Endosome 1.85 2.64 2.39 3.92 -3.86 -6.16 5.91 4.51 9.39 -0.71 -5.36 8.52 5.76 10.61 -3.17
Golgi 0.09 2.37 1.85 5.55 -3.24 1.58 14.45 6.40 5.57 -1.78 1.80 15.60 9.86 9.48 -3.32
Lipid Particles -0.33 1.50 1.07 3.92 -3.61 0.32 9.53 10.60 11.50 2.17 0.61 10.45 9.40 10.75 -2.12
Mitochondria 0.16 2.42 0.44 0.22 -2.30 -3.38 1.86 0.76 4.27 -1.61 -3.22 2.06 -0.03 3.45 -2.69
Mitotic Spindle -1.37 -5.48 -3.61 -7.73 12.74 1.83 -23.16* -14.14 -16.74 5.94 0.04 -31.32 -19.45 -22.42 12.81
None -1.01 1.32 0.09 1.17 -1.86 -1.02 4.17 5.16 6.54 3.05 -0.77 4.75 3.91 5.21 -0.98
Nuclear Periphery 2.16 2.22 2.07 -1.45 -2.43 -1.62 2.69 5.28 7.58 3.90 -0.03 4.74 4.41 5.64 -0.19
Nuclear Periphery Foci 0.89 2.26 2.34 7.51 1.57 3.64 14.19 19.54* 18.18* 7.79 4.58 17.19 19.78 19.70 7.09
Nucleolus 0.73 2.10 2.09 3.00 -0.01 1.02 5.99 10.51 10.95 6.48 1.67 8.29 10.21 10.74 4.46
Nucleus 1.97 2.15 2.12 1.31 -0.42 -1.68 1.03 5.17 8.00 4.61 -0.63 3.24 4.89 7.21 2.74
Peroxisomes -3.46 0.97 -0.27 6.36 -5.32 2.57 11.84 8.93 7.20 -1.81 1.88 14.25 10.48 10.32 -4.99
Vacuole 2.75 2.93 2.32 -3.35 -5.13 -0.42 1.04 -3.40 -3.27 -3.97 0.52 3.07 -3.45 -4.21 -6.28
Vacuole Periphery 2.13 2.15 1.81 -2.79 -2.86 -4.84 0.67 -2.23 -1.71 -2.27 -1.54 1.81 -3.00 -2.62 -3.51
Standard name
Human Ortholog
Description Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane
Localization
Cell Percentages spindle pole (89%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Spc42

Spc42


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Spc42-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available