Standard name
Human Ortholog
Description One of four subunits of the ESCRT-III complex; forms an endosomal sorting complex required for transport III (ESCRT-III) subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0.06 0 0 0 0.05 0.06 0.07 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.71 0.57 0.53 0.3 0.18 0.13 0.09 0.06 0 0.05 0.05 0.89 0.87 0.94 0.51 0.51 0.06 0.26 0.13 0.15 0.2 0.28
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.08 0 0 0 0 0 0 0
Endosome 0.05 0.06 0 0.05 0 0 0 0 0 0 0 0 0 0 0.1 0.11 0.07 0.08 0.08 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.21 0.33 0.4 0.69 0.87 0.91 0.89 0.93 0.94 0.96 0.96 0 0 0 0.15 0.07 0.7 0.45 0.56 0.55 0.46 0.37
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
SpindlePole 0 0.09 0.11 0.07 0 0.05 0.08 0.07 0.07 0 0 0 0.05 0 0.21 0.19 0 0.06 0.08 0 0 0
Vac/Vac Membrane 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0.08 0.11 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 1 0 0 0 0 0 0 0 0 0 2 1 1 5 6 1 9 9 7 7 8
Bud 5 14 6 10 21 27 31 29 20 29 22 1 0 0 9 9 0 4 1 3 3 9
Bud Neck 3 2 0 1 3 1 1 0 0 0 1 2 6 2 4 10 0 4 2 4 6 8
Bud Site 1 1 0 0 11 8 11 8 7 18 4 0 0 0 1 1
Cell Periphery 3 0 3 1 3 2 1 1 4 1 2 2 2 2 1 10 1 2 1 1 1 2
Cytoplasm 224 143 126 78 92 67 46 24 14 26 23 181 183 242 124 120 2 104 43 32 49 89
Endoplasmic Reticulum 3 1 0 0 1 1 1 3 2 0 1 10 5 2 19 10 0 2 2 6 6 6
Endosome 15 14 10 13 2 0 9 1 4 0 1 3 6 7 24 25 3 30 25 8 10 13
Golgi 0 0 1 0 0 0 0 0 0 0 0 1 0 0 2 2 0 3 0 1 3 5
Mitochondria 65 81 96 177 440 472 470 380 434 514 443 0 1 0 37 17 32 176 183 118 111 117
Nucleus 0 2 3 1 6 8 12 8 4 8 1 2 1 1 1 5 0 0 0 0 1 1
Nuclear Periphery 2 0 0 1 0 1 0 1 8 4 9 0 0 0 1 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 1 1 7 4 1 3 3 0 0 0 0 0 0 0 1 0 1 0
Peroxisomes 7 11 15 2 8 10 10 9 9 4 8 0 0 2 12 1 0 1 7 3 2 3
SpindlePole 13 23 25 19 22 26 40 28 33 24 13 7 10 6 50 45 1 25 27 6 5 10
Vac/Vac Membrane 11 4 3 14 13 8 10 9 17 13 12 0 5 9 12 14 1 17 6 17 27 27
Unique Cell Count 315 249 238 258 504 521 526 409 463 534 462 204 210 258 241 234 46 395 327 217 244 318
Labelled Cell Count 355 297 288 317 623 632 649 505 557 644 543 211 220 274 302 275 46 395 327 217 244 318


Endosome, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 3.8 3.7 3.5 3.4 3.0 3.4 3.1 3.0 3.1 3.2 5.4 4.9 4.9 5.0 5.0 4.8 5.6 5.8 5.8
Std Deviation (1e-4) 0.6 1.3 1.3 1.6 0.9 1.2 1.5 0.9 0.6 1.0 0.8 1.5 1.1 1.0 1.0 1.3 1.1 1.8 1.7 2.3
Intensity Change (Log2) -0.05 -0.09 -0.14 -0.33 -0.14 -0.27 -0.32 -0.28 -0.24 0.53 0.37 0.38 0.4 0.41 0.35 0.58 0.62 0.62


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0
Bud Neck 0 0
Bud Site 0 0
Cell Periphery 0 0
Cyto
Endoplasmic Reticulum 0 0
Endosome 0 0
Golgi 0 0
Mitochondria 0 0
Nuclear Periphery 0 0
Nuc
Nucleolus 0 0
Peroxisomes 0 0
SpindlePole 0 0
Vac
Cortical Patches 0 0
Cytoplasm 0 0
Nucleus 0 0
Vacuole 2.1 2.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.4026 0.476 0.5249 0.2809 0.3456 0.5367 1.5677 2.4794 2.1571 2.3653 1.8418 2.6958 0.227 0.4791 0.4116 0.3655 0.3304 0.4707
Actin 0.0081 0.0032 0.0044 0.0057 0.0021 0.0199 0.0266 0.0457 0.0113 0.0051 0.0499 0.0132 0.0099 0.0076 0.0092 0.0126 0.0044 0.007
Bud 0.0018 0.0031 0.0075 0.0026 0.0005 0.0028 0.0015 0.0039 0.0061 0.0011 0.0027 0.0021 0.0014 0.0039 0.0025 0.0012 0.0026 0.0015
Bud Neck 0.002 0.0013 0.0066 0.0013 0.0007 0.0034 0.0024 0.0042 0.003 0.0011 0.0013 0.0146 0.0018 0.0043 0.0032 0.0203 0.0021 0.0055
Bud Periphery 0.0041 0.0081 0.012 0.009 0.0016 0.0083 0.0048 0.0113 0.0144 0.0024 0.0066 0.0069 0.0041 0.0082 0.0085 0.0044 0.0097 0.0064
Bud Site 0.0061 0.0245 0.0117 0.0101 0.0005 0.0025 0.0059 0.0576 0.0269 0.0019 0.0023 0.0029 0.0085 0.0141 0.0065 0.0035 0.0024 0.0017
Cell Periphery 0.0017 0.0019 0.0013 0.0015 0.0003 0.0011 0.0028 0.0031 0.0023 0.0007 0.0022 0.0023 0.0012 0.001 0.0021 0.0006 0.0018 0.0022
Cytoplasm 0.0489 0.0524 0.0673 0.0388 0.0219 0.0419 0.0998 0.0674 0.1053 0.0515 0.0439 0.1058 0.0465 0.0409 0.0625 0.0228 0.0506 0.0541
Cytoplasmic Foci 0.084 0.0802 0.0861 0.0939 0.0729 0.0879 0.092 0.1072 0.1391 0.0687 0.1034 0.1101 0.109 0.1239 0.0897 0.1196 0.1407 0.1297
Eisosomes 0.0008 0.0007 0.0007 0.0008 0.0004 0.0005 0.0045 0.0019 0.0012 0.0008 0.002 0.0009 0.0011 0.0005 0.0008 0.0003 0.0004 0.0004
Endoplasmic Reticulum 0.0071 0.0051 0.004 0.0031 0.0024 0.0019 0.0054 0.0034 0.0041 0.0011 0.0036 0.0018 0.0027 0.0017 0.0026 0.0012 0.0011 0.0031
Endosome 0.1413 0.1358 0.0885 0.1348 0.0975 0.0862 0.1078 0.1233 0.1126 0.0362 0.0817 0.078 0.1612 0.1285 0.0936 0.0846 0.1531 0.1322
Golgi 0.0429 0.044 0.0312 0.0269 0.0393 0.0341 0.046 0.0553 0.042 0.0486 0.0767 0.0383 0.0547 0.0694 0.0352 0.0263 0.042 0.0422
Lipid Particles 0.0424 0.0216 0.0249 0.0181 0.0329 0.0462 0.0349 0.0175 0.0199 0.0041 0.0384 0.0201 0.0491 0.013 0.0198 0.0634 0.0137 0.0317
Mitochondria 0.3629 0.4205 0.3929 0.4829 0.4278 0.402 0.2403 0.2765 0.2682 0.3332 0.3343 0.3321 0.3996 0.4376 0.4351 0.468 0.4302 0.3892
None 0.1545 0.1271 0.1835 0.1151 0.1583 0.178 0.2637 0.1381 0.1721 0.3477 0.1715 0.1844 0.0818 0.0559 0.1433 0.0852 0.0499 0.093
Nuclear Periphery 0.0112 0.0025 0.0027 0.0013 0.0295 0.0009 0.0098 0.0013 0.002 0.0005 0.0006 0.0005 0.002 0.0006 0.0011 0.0005 0.0005 0.002
Nucleolus 0.0036 0.0029 0.0016 0.0024 0.0006 0.001 0.0017 0.0009 0.0008 0.0007 0.001 0.0009 0.0026 0.0008 0.0018 0.0004 0.0009 0.0018
Nucleus 0.0109 0.0026 0.0034 0.0023 0.0127 0.0015 0.0018 0.0012 0.0011 0.0011 0.001 0.001 0.002 0.0011 0.0019 0.0004 0.0018 0.0026
Peroxisomes 0.0377 0.0353 0.0492 0.0289 0.0865 0.0675 0.0306 0.0655 0.0514 0.0875 0.0627 0.0707 0.042 0.0751 0.0566 0.0779 0.0733 0.0751
Punctate Nuclear 0.0064 0.0022 0.006 0.003 0.0008 0.0026 0.004 0.0052 0.0018 0.0005 0.0006 0.001 0.0042 0.0005 0.0017 0.0018 0.0012 0.0033
Vacuole 0.0154 0.019 0.0104 0.0124 0.0054 0.0064 0.0096 0.0058 0.0106 0.0038 0.0101 0.0086 0.0106 0.0086 0.0152 0.0029 0.0133 0.0113
Vacuole Periphery 0.0063 0.0059 0.0041 0.0052 0.0054 0.0034 0.004 0.0034 0.0037 0.0015 0.0033 0.0036 0.0039 0.0028 0.007 0.0024 0.0043 0.0043

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.2693 5.8452 9.7591 12.7769 18.0151 3.2675 8.1263 11.4681 10.6711 9.5598
Translational Efficiency 16.0356 8.3164 4.8197 4.2594 2.9194 15.2625 6.3363 4.0969 4.3749 5.0008

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1792 1541 170 1205 1732 1849 2449 1850 3524 3390 2619 3055

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 547.81 682.79 824.66 769.97 645.25 685.93 767.97 831.50 595.70 684.50 771.65 807.23
Standard Deviation 59.60 90.67 82.11 117.62 95.87 83.03 91.57 111.01 93.25 86.60 92.05 117.57
Intensity Change Log 2 0.317766 0.590124 0.491127 0.088203 0.251192 0.365858 0.198161 0.416745 0.424730

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000301 0.002112 0.003922 0.001498 0.000476 0.001108 0.001762 0.003638 0.000387 0.001565 0.001902 0.002794
Bud Neck 0.061323 0.053822 0.036854 0.021760 0.047476 0.039566 0.084968 0.034081 0.054517 0.046046 0.081845 0.029222
Bud Site 0.016425 0.035675 0.039383 0.036352 0.016343 0.028048 0.059970 0.057821 0.016384 0.031515 0.058634 0.049353
Cell Periphery 0.000457 0.000226 0.000250 0.000312 0.000427 0.000207 0.000131 0.000252 0.000443 0.000215 0.000139 0.000276
Cytoplasm 0.154000* 0.082573 0.050379 0.348534* 0.129047* 0.069449 0.181513* 0.374121* 0.141736* 0.075415 0.173001* 0.364028*
Cytoplasmic Foci 0.315553* 0.245168* 0.147638 0.079803 0.303629* 0.227782* 0.101920 0.107594 0.309692* 0.235685* 0.104888 0.096632
Eisosomes 0.000467 0.000278 0.000219 0.000109 0.000684 0.000240 0.000090 0.000134 0.000574 0.000257 0.000098 0.000124
Endoplasmic Reticulum 0.002140 0.000768 0.008636 0.003242 0.002298 0.000500 0.001791 0.001906 0.002218 0.000622 0.002235 0.002433
Endosome 0.023658 0.070442 0.097635 0.018415 0.060981 0.089696 0.047925 0.016097 0.042002 0.080944 0.051152 0.017011
Golgi 0.030454 0.065230 0.031127 0.003701 0.048422 0.088117 0.012710 0.007260 0.039285 0.077713 0.013905 0.005856
Lipid Particles 0.038593 0.008464 0.018351 0.009975 0.032910 0.010795 0.004192 0.003348 0.035800 0.009735 0.005111 0.005962
Mitochondria 0.237312* 0.251792* 0.082136 0.009530 0.212315* 0.293599* 0.105793* 0.017348 0.225027* 0.274595* 0.104257* 0.014264
Mitotic Spindle 0.001364 0.015496 0.129837* 0.042460 0.005571 0.013414 0.097949 0.033773 0.003432 0.014360 0.100019* 0.037200
None 0.004169 0.001828 0.000955 0.006607 0.012527 0.000763 0.001905 0.001726 0.008277 0.001247 0.001843 0.003652
Nuclear Periphery 0.000383 0.000234 0.001872 0.002563 0.000391 0.000263 0.001548 0.000894 0.000387 0.000250 0.001569 0.001552
Nuclear Periphery Foci 0.000185 0.000250 0.005063 0.001804 0.000385 0.000210 0.000455 0.000494 0.000283 0.000228 0.000754 0.001011
Nucleolus 0.001539 0.001732 0.014808 0.004346 0.002752 0.001608 0.004320 0.001130 0.002135 0.001664 0.005001 0.002399
Nucleus 0.049579 0.013354 0.102534 0.226399* 0.036882 0.010463 0.085826 0.167084* 0.043339 0.011777 0.086910 0.190480*
Peroxisomes 0.039060 0.107154* 0.091468 0.019778 0.045037 0.073671 0.053437 0.043090 0.041998 0.088891 0.055906 0.033895
Vacuole 0.017260 0.035761 0.123637 0.158436 0.032760 0.039537 0.145453 0.126098 0.024878 0.037820 0.144037 0.138854
Vacuole Periphery 0.005776 0.007643 0.013297 0.004375 0.008686 0.010968 0.006341 0.002111 0.007206 0.009456 0.006793 0.003004

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.94 -3.84 -3.96 1.96 2.79 -5.09 -5.57 -4.54 -3.28 -2.06 -6.53 -6.90 -5.55 -1.76 -0.96
Bud Neck 1.80 4.58 13.11 10.38 3.33 2.29 -9.49 5.43 3.37 16.75 3.15 -7.96 12.58 9.41 19.49
Bud Site -8.74 -3.75 -4.21 1.72 1.42 -5.57 -15.45 -10.09 -5.32 2.82 -9.94 -16.52 -10.67 -3.14 5.11
Cell Periphery 5.67 3.89 5.11 -0.30 0.48 5.45 7.93 5.41 0.22 -4.08 7.90 11.18 7.57 0.07 -5.36
Cytoplasm 11.91 9.87 -18.15 -27.13 -22.38 10.70 -9.13 -28.67 -39.24 -20.79 16.23 -6.87 -33.41 -47.43 -23.99
Cytoplasmic Foci 9.13 13.61 39.75 29.48 6.88 10.42 32.92 32.76 23.71 1.59 14.01 43.74 50.11 36.60 5.69
Eisosomes 5.60 9.78 18.19 6.16 8.46 2.75 3.69 3.49 5.77 -4.25 3.91 5.96 5.75 8.33 -3.06
Endoplasmic Reticulum 4.42 -4.44 -2.62 -6.69 3.67 5.53 0.91 0.53 -9.09 -0.59 7.16 -0.93 -1.34 -10.51 -0.56
Endosome -15.32 -7.43 6.18 18.11 8.46 -7.26 5.56 18.18 24.36 15.90 -15.38 -2.24 18.66 30.34 17.79
Golgi -11.15 0.48 18.33 22.54 5.03 -10.18 16.10 18.82 25.38 6.29 -15.14 17.95 25.35 33.47 9.45
Lipid Particles 14.22 6.12 13.48 0.93 4.13 10.02 14.36 15.07 7.41 3.61 16.91 21.09 20.88 6.34 0.95
Mitochondria -1.24 11.08 32.84* 32.76* 5.15 -8.38 15.78 29.34* 39.16* 22.57* -6.94 22.72 43.76* 50.80* 24.44*
Mitotic Spindle -8.16 -6.93 -9.06 -5.25 4.65 -4.80 -22.53* -8.46 -4.89 15.08 -9.32 -24.44 -12.71 -7.11 14.87
None 3.85 5.67 -0.64 -3.03 -3.94 6.03 5.45 5.52 -6.13 0.15 7.01 6.37 4.90 -4.15 -2.68
Nuclear Periphery 2.13 -5.20 -9.60 -10.12 -1.82 1.94 -10.84 -11.96 -12.08 2.67 2.83 -11.75 -13.96 -14.80 -0.85
Nuclear Periphery Foci -1.24 -2.95 -9.23 -8.44 2.27 2.71 -1.90 -3.77 -6.70 -1.13 1.18 -4.05 -10.33 -10.44 -0.58
Nucleolus -0.67 -4.75 -2.41 -2.05 4.16 1.97 -3.32 2.41 0.18 7.72 1.48 -7.25 0.01 -1.33 6.89
Nucleus 13.58 -4.39 -24.67 -30.66 -7.68 9.82 -14.75 -27.66 -35.10 -15.65 16.65 -15.20 -36.82 -46.43 -20.72
Peroxisomes -14.51 -4.33 9.26 19.43 6.52 -7.51 -1.61 3.70 10.28 5.13 -15.76 -3.92 7.50 20.32 9.50
Vacuole -8.72 -9.63 -28.68 -25.51 -6.97 -2.69 -30.89 -28.69 -26.50 -3.22 -7.80 -35.51 -40.95 -36.73 -7.62
Vacuole Periphery -3.24 -3.19 3.98 5.77 4.50 -2.58 4.67 11.46 12.51 8.78 -4.16 2.44 11.44 13.61 8.29
Standard name
Human Ortholog
Description One of four subunits of the ESCRT-III complex; forms an endosomal sorting complex required for transport III (ESCRT-III) subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway
Localization
Cell Percentages mitochondrion (47%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Vps24

Vps24


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vps24-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available