Standard name
Description One of four subunits of the ESCRT-III complex; forms an endosomal sorting complex required for transport III (ESCRT-III) subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0.06 0 0 0 0.05 0.06 0.07 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.71 0.57 0.53 0.3 0.18 0.13 0.09 0.06 0 0.05 0.05 0.89 0.87 0.94 0.51 0.51 0.06 0.26 0.13 0.15 0.2 0.28
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.08 0 0 0 0 0 0 0
Endosome 0.05 0.06 0 0.05 0 0 0 0 0 0 0 0 0 0 0.1 0.11 0.07 0.08 0.08 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.21 0.33 0.4 0.69 0.87 0.91 0.89 0.93 0.94 0.96 0.96 0 0 0 0.15 0.07 0.7 0.45 0.56 0.55 0.46 0.37
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
SpindlePole 0 0.09 0.11 0.07 0 0.05 0.08 0.07 0.07 0 0 0 0.05 0 0.21 0.19 0 0.06 0.08 0 0 0
Vac/Vac Membrane 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0.08 0.11 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 1 0 0 0 0 0 0 0 0 0 2 1 1 5 6 1 9 9 7 7 8
Bud 5 14 6 10 21 27 31 29 20 29 22 1 0 0 9 9 0 4 1 3 3 9
Bud Neck 3 2 0 1 3 1 1 0 0 0 1 2 6 2 4 10 0 4 2 4 6 8
Bud Site 1 1 0 0 11 8 11 8 7 18 4 0 0 0 1 1
Cell Periphery 3 0 3 1 3 2 1 1 4 1 2 2 2 2 1 10 1 2 1 1 1 2
Cytoplasm 224 143 126 78 92 67 46 24 14 26 23 181 183 242 124 120 2 104 43 32 49 89
Endoplasmic Reticulum 3 1 0 0 1 1 1 3 2 0 1 10 5 2 19 10 0 2 2 6 6 6
Endosome 15 14 10 13 2 0 9 1 4 0 1 3 6 7 24 25 3 30 25 8 10 13
Golgi 0 0 1 0 0 0 0 0 0 0 0 1 0 0 2 2 0 3 0 1 3 5
Mitochondria 65 81 96 177 440 472 470 380 434 514 443 0 1 0 37 17 32 176 183 118 111 117
Nucleus 0 2 3 1 6 8 12 8 4 8 1 2 1 1 1 5 0 0 0 0 1 1
Nuclear Periphery 2 0 0 1 0 1 0 1 8 4 9 0 0 0 1 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 1 1 7 4 1 3 3 0 0 0 0 0 0 0 1 0 1 0
Peroxisomes 7 11 15 2 8 10 10 9 9 4 8 0 0 2 12 1 0 1 7 3 2 3
SpindlePole 13 23 25 19 22 26 40 28 33 24 13 7 10 6 50 45 1 25 27 6 5 10
Vac/Vac Membrane 11 4 3 14 13 8 10 9 17 13 12 0 5 9 12 14 1 17 6 17 27 27
Unique Cell Count 315 249 238 258 504 521 526 409 463 534 462 204 210 258 241 234 46 395 327 217 244 318
Labelled Cell Count 355 297 288 317 623 632 649 505 557 644 543 211 220 274 302 275 46 395 327 217 244 318


Endosome, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 3.8 3.7 3.5 3.4 3.0 3.4 3.1 3.0 3.1 3.2 5.4 4.9 4.9 5.0 5.0 4.8 5.6 5.8 5.8
Std Deviation (1e-4) 0.6 1.3 1.3 1.6 0.9 1.2 1.5 0.9 0.6 1.0 0.8 1.5 1.1 1.0 1.0 1.3 1.1 1.8 1.7 2.3
Intensity Change (Log2) -0.05 -0.09 -0.14 -0.33 -0.14 -0.27 -0.32 -0.28 -0.24 0.53 0.37 0.38 0.4 0.41 0.35 0.58 0.62 0.62


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0
Bud Neck 0 0
Bud Site 0 0
Cell Periphery 0 0
Cyto
Endoplasmic Reticulum 0 0
Endosome 0 0
Golgi 0 0
Mitochondria 0 0
Nuclear Periphery 0 0
Nuc
Nucleolus 0 0
Peroxisomes 0 0
SpindlePole 0 0
Vac
Cortical Patches 0 0
Cytoplasm 0 0
Nucleus 0 0
Vacuole 2.1 2.5


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vps24-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available