Standard name
Human Ortholog
Description Phospholipid hydroperoxide glutathione peroxidase; induced by glucose starvation that protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress; GPX1 has a paralog, HYR1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.97 – 1.0 0.99 0.95 0.96 – 0.85 0.83 0.78 0.82 – – – 0.99 0.98 0.98 0.9 0.95 0.87 0.89 0.92 0.92
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0.07 0.07 0.08 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.11 – 0 0 0.08 0 – 0.22 0.17 0.31 0.22 – – – 0 0 0 0.06 0 0 0.05 0 0
Nucleus 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 1 0 1 0 – 0 1 0 0 – – – 0 0 0 0 0 0 0 2 2
Bud 0 1 – 0 0 1 0 – 2 3 6 10 – – – 0 0 1 0 0 0 0 0 1
Bud Neck 0 0 – 2 7 7 17 – 7 14 5 10 – – – 1 1 1 0 0 0 0 1 0
Bud Site 0 0 – 0 0 0 0 – 0 0 2 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 1 – 1 0 2 4 – 2 1 3 3 – – – 9 13 4 0 0 0 0 1 1
Cytoplasm 221 34 – 290 365 243 549 – 324 414 271 332 – – – 271 297 216 208 55 42 127 262 356
Endoplasmic Reticulum 1 0 – 0 0 0 0 – 0 1 0 0 – – – 20 21 18 0 0 0 0 0 0
Endosome 2 0 – 0 0 0 3 – 0 0 3 1 – – – 0 0 0 4 0 0 0 0 3
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 1 0 0 0 0 0 0
Mitochondria 5 4 – 2 2 20 25 – 85 84 109 89 – – – 3 3 7 13 1 2 6 11 9
Nucleus 0 1 – 0 2 0 2 – 2 9 2 6 – – – 2 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 – 0 1 0 2 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 1 – 1 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 1 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 1 2 10 – 10 6 5 10 – – – 1 0 1 1 0 0 0 2 3
Vac/Vac Membrane 0 0 – 1 3 0 11 – 9 20 6 14 – – – 0 3 1 1 1 1 5 0 2
Unique Cell Count 226 35 290 367 255 573 382 501 347 407 273 302 220 233 58 49 143 284 387
Labelled Cell Count 229 41 297 380 276 622 442 554 412 477 307 339 250 233 58 49 143 284 387


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 3.6 – 4.9 5.5 3.8 4.4 – 3.8 4.0 3.9 4.1 – – – 5.0 5.4 5.0 3.8 4.0 4.1
Std Deviation (1e-4) 0.3 0.5 – 1.7 2.1 1.6 1.3 – 1.4 1.2 1.2 1.3 – – – 1.0 1.1 1.1 1.0 0.7 0.7
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.333 -0.9815 -1.1636 -0.9255 -1.5743 -1.0828 -0.8147 0.2926 -0.1101 -0.3793 -0.1119 0.0772 1.0859 1.3839 1.0388 1.7877 1.8954 1.3857
Actin 0.0327 0.0001 0.0007 0.0355 0.0003 0.0052 0.0092 0.0005 0.0063 0.0002 0.0009 0.0003 0.0313 0.0005 0.0014 0.0031 0.0003 0.0038
Bud 0.0042 0 0.0089 0.0007 0.0001 0.0004 0 0.0014 0.0007 0.0003 0.0003 0.0001 0.0133 0.0243 0.0112 0.009 0 0.0088
Bud Neck 0.0027 0 0.0001 0.001 0 0.0016 0.0001 0.0001 0.0003 0.0001 0.0001 0.0002 0.0052 0.0003 0.0002 0.0004 0 0.0007
Bud Periphery 0.0091 0 0.0053 0.002 0.0001 0.0011 0.0001 0.0024 0.0006 0.0008 0.0004 0.0001 0.0062 0.0052 0.0042 0.0053 0.0001 0.0049
Bud Site 0.0075 0.0001 0.0024 0.0031 0 0.0002 0.0007 0.0038 0.0004 0 0.0001 0 0.0066 0.0348 0.0062 0.0013 0 0.0003
Cell Periphery 0.0005 0 0.0006 0.0002 0 0.0001 0.0002 0.0002 0.0001 0 0 0 0.0006 0.001 0.0003 0.0002 0 0.0002
Cytoplasm 0.0545 0.0668 0.0448 0.0478 0.0314 0.0741 0.1472 0.2824 0.2263 0.2519 0.1836 0.2771 0.0625 0.1112 0.1257 0.0988 0.0509 0.1358
Cytoplasmic Foci 0.0194 0.0033 0.0096 0.0288 0.0057 0.0051 0.0056 0.005 0.0074 0.0069 0.016 0.0037 0.0244 0.0023 0.0049 0.0048 0.0567 0.0103
Eisosomes 0.0006 0.0001 0.0002 0.0007 0.0001 0.0001 0.0003 0.0002 0.0001 0 0.0001 0 0.001 0.0001 0.0002 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0034 0.0003 0.0011 0.0017 0.0002 0.0012 0.0011 0.0009 0.001 0.0011 0.001 0.0005 0.0014 0.0006 0.0021 0.0066 0.0009 0.0013
Endosome 0.0059 0.0002 0.0021 0.0103 0.0001 0.0047 0.002 0.0009 0.0023 0.0011 0.0019 0.0005 0.0047 0.0002 0.001 0.0054 0.0017 0.0016
Golgi 0.0033 0 0.0003 0.0029 0 0.0005 0.0003 0.0001 0.0004 0.0001 0.0002 0 0.0036 0 0.0002 0.0007 0.0001 0.0006
Lipid Particles 0.007 0.0002 0.0025 0.0083 0.0003 0.0021 0.0005 0.0001 0.0004 0.0001 0.0006 0.0001 0.0022 0.0001 0.0003 0.0015 0.0015 0.0003
Mitochondria 0.0035 0.0001 0.0019 0.0033 0.0002 0.0008 0.0002 0.0003 0.0003 0.0004 0.0005 0.0001 0.0072 0.0006 0.0012 0.0207 0.0007 0.0007
None 0.8229 0.9272 0.9059 0.8057 0.9599 0.8922 0.8285 0.6992 0.7486 0.7332 0.7895 0.7159 0.8117 0.8067 0.8353 0.8176 0.8366 0.8208
Nuclear Periphery 0.0036 0.0001 0.0013 0.0029 0.0002 0.0011 0.0004 0.0003 0.0004 0.0007 0.0006 0.0002 0.0009 0.0009 0.0006 0.0043 0.0325 0.0019
Nucleolus 0.0026 0.0001 0.0011 0.0017 0.0001 0.0003 0.0002 0.0001 0.0001 0.0001 0.0001 0 0.0019 0.0006 0.0004 0.001 0.0003 0.0004
Nucleus 0.0023 0.0003 0.0028 0.0047 0.0001 0.0009 0.0008 0.0008 0.0007 0.0011 0.0007 0.0003 0.0021 0.0073 0.0019 0.0044 0.0091 0.0021
Peroxisomes 0.0064 0.0004 0.0008 0.0137 0.0002 0.002 0.0002 0.0002 0.0017 0.0001 0.0007 0 0.0054 0.0001 0.0003 0.0099 0.0001 0.0002
Punctate Nuclear 0.0055 0.0002 0.0046 0.0234 0.0007 0.0056 0.0018 0.0003 0.0005 0.0009 0.0014 0.0004 0.0055 0.0025 0.0016 0.0035 0.0066 0.0044
Vacuole 0.0017 0.0002 0.0023 0.0011 0.0001 0.0004 0.0007 0.0008 0.0013 0.0007 0.0008 0.0003 0.0016 0.0007 0.0006 0.0011 0.0005 0.0006
Vacuole Periphery 0.0008 0 0.0006 0.0004 0 0.0002 0.0001 0.0001 0.0002 0.0002 0.0002 0 0.0006 0.0001 0.0001 0.0004 0.0012 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
988 1145 621 180 417 1003 208 326 1405 2148 829 506

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 639.38 667.32 863.03 823.95 683.87 723.35 848.05 841.06 652.58 693.48 859.27 834.97
Standard Deviation 75.43 88.92 88.41 108.88 72.58 85.04 107.24 110.41 77.31 91.50 93.72 110.17
Intensity Change Log 2 — 0.061705 0.432737 0.365883 — 0.080972 0.310427 0.298487 — 0.071694 0.370822 0.331445

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000100 0.000717 0.000215 0.000439 0.000078 0.000175 0.000465 0.000423 0.000093 0.000464 0.000278 0.000429
Bud Neck 0.017456 0.039209 0.002394 0.002193 0.007065 0.033942 0.001218 0.002839 0.014372 0.036750 0.002099 0.002609
Bud Site 0.003052 0.026164 0.004535 0.013865 0.002422 0.009224 0.002265 0.019584 0.002865 0.018254 0.003965 0.017550
Cell Periphery 0.000190 0.000137 0.000143 0.000103 0.000181 0.000206 0.000294 0.000143 0.000187 0.000170 0.000181 0.000129
Cytoplasm 0.432327* 0.399982* 0.364217* 0.439929* 0.525651* 0.444172* 0.387335* 0.495533* 0.460026* 0.420616* 0.370017* 0.475753*
Cytoplasmic Foci 0.284403* 0.262164* 0.016142 0.031932 0.242008* 0.239288* 0.047906 0.037573 0.271820* 0.251482* 0.024112 0.035566
Eisosomes 0.000266 0.000123 0.000050 0.000054 0.000156 0.000199 0.000123 0.000049 0.000234 0.000159 0.000069 0.000051
Endoplasmic Reticulum 0.000694 0.001111 0.003281 0.002288 0.000462 0.000877 0.005002 0.002057 0.000625 0.001002 0.003713 0.002139
Endosome 0.014926 0.036309 0.002955 0.005825 0.008179 0.017668 0.006536 0.004936 0.012924 0.027605 0.003853 0.005252
Golgi 0.002977 0.006498 0.000066 0.007932 0.000991 0.005556 0.000124 0.001569 0.002388 0.006058 0.000081 0.003832
Lipid Particles 0.017954 0.007630 0.001839 0.001439 0.006851 0.008464 0.004509 0.001133 0.014659 0.008020 0.002508 0.001242
Mitochondria 0.010933 0.012486 0.000398 0.000286 0.000720 0.008996 0.000241 0.000329 0.007902 0.010856 0.000359 0.000314
Mitotic Spindle 0.000390 0.003194 0.004796 0.009373 0.000290 0.002940 0.000931 0.008550 0.000361 0.003075 0.003826 0.008843
None 0.003047 0.003244 0.003631 0.003671 0.002482 0.004858 0.003777 0.007510 0.002879 0.003998 0.003668 0.006144
Nuclear Periphery 0.000634 0.000453 0.001808 0.001190 0.000284 0.000347 0.001180 0.000999 0.000530 0.000403 0.001651 0.001067
Nuclear Periphery Foci 0.000340 0.000636 0.001401 0.002980 0.000462 0.000198 0.004573 0.000848 0.000376 0.000432 0.002196 0.001606
Nucleolus 0.001658 0.002777 0.000516 0.000349 0.000713 0.001398 0.000952 0.000291 0.001378 0.002133 0.000625 0.000312
Nucleus 0.157044* 0.090803 0.526406* 0.361603* 0.166646* 0.151716* 0.402277* 0.265746* 0.159894* 0.119246 0.495261* 0.299846*
Peroxisomes 0.009283 0.019686 0.000280 0.000297 0.002838 0.013787 0.000605 0.000510 0.007370 0.016932 0.000361 0.000434
Vacuole 0.040476 0.082847 0.064767 0.114090 0.031086 0.054404 0.129475 0.149165 0.037689 0.069566 0.081002 0.136688
Vacuole Periphery 0.001849 0.003832 0.000160 0.000160 0.000437 0.001581 0.000212 0.000213 0.001430 0.002781 0.000173 0.000194

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.50 -5.03 -1.68 2.01 0.33 -3.25 -5.95 -4.20 -2.89 1.63 -3.88 -7.43 -4.21 0.84 0.43
Bud Neck -6.73 7.48 7.55 13.86 0.40 -8.07 3.18 2.12 10.64 -6.20 -9.32 8.04 7.71 17.33 -1.67
Bud Site -11.00 -1.07 -1.12 3.86 -0.67 -4.75 -0.67 -2.56 -0.32 -2.45 -11.73 -1.19 -2.65 3.20 -1.89
Cell Periphery 3.48 1.78 6.18 4.50 4.98 -0.98 -3.52 3.54 3.56 5.68 1.27 -0.10 5.62 4.32 5.69
Cytoplasm 2.88 8.66 3.95 2.45 -1.17 5.62 8.88 8.35 4.32 -1.30 4.39 12.16 7.18 4.39 -2.56
Cytoplasmic Foci 2.31 38.50 34.23 33.01 -2.19 0.27 19.04 20.88 27.38 1.70 2.75 42.87 40.16 42.53 -2.25
Eisosomes 7.60 11.13 10.63 6.11 -0.71 -2.83 2.05 8.55 11.60 5.87 5.24 11.38 12.73 13.14 2.89
Endoplasmic Reticulum -2.34 -12.26 -6.66 -5.53 3.28 -1.39 -8.18 -9.03 -5.76 4.17 -2.16 -14.78 -10.67 -8.18 5.44
Endosome -7.79 7.98 6.34 12.68 -0.43 -5.52 1.80 3.68 8.40 2.45 -8.29 8.24 7.50 15.05 0.96
Golgi -4.68 8.01 0.01 1.16 -1.00 -4.82 3.85 0.69 4.70 -0.93 -5.95 8.70 0.86 3.77 -1.25
Lipid Particles 5.47 9.26 9.67 8.00 2.68 -1.22 2.12 5.22 8.68 5.19 4.84 9.53 10.66 11.84 5.90
Mitochondria -0.53 5.08 5.18 8.34 2.89 -7.50 3.68 3.30 7.92 -1.04 -1.65 5.15 5.24 11.33 2.47
Mitotic Spindle -5.07 -2.22 -1.35 -0.86 -0.61 -2.50 -0.84 -2.16 -1.36 -1.89 -4.73 -2.33 -2.50 -1.60 -1.32
None -0.10 -0.15 -0.14 -0.06 -0.02 -2.34 -1.06 -2.58 -0.06 -1.76 -1.42 -0.49 -1.76 -0.46 -1.74
Nuclear Periphery 1.82 -13.95 -6.61 -7.64 2.13 -1.63 -11.98 -12.13 -11.36 -1.76 1.93 -16.80 -11.25 -13.09 2.82
Nuclear Periphery Foci -3.13 -6.67 -1.49 -1.33 -0.69 3.22 -7.75 -5.38 -7.75 6.12 -1.09 -9.83 -2.26 -2.16 0.82
Nucleolus -2.56 4.09 4.73 6.54 2.87 -2.59 -0.24 4.30 4.52 4.13 -2.64 4.00 5.47 7.96 5.30
Nucleus 9.85 -29.13 -8.21 -11.45 8.29 1.46 -11.53 -6.99 -8.43 5.45 7.17 -30.65 -11.09 -14.81 13.47
Peroxisomes -5.56 8.72 8.98 12.69 1.18 -7.07 3.64 3.56 9.29 0.23 -7.31 9.29 9.31 15.58 0.23
Vacuole -10.91 -9.37 -10.30 -8.09 -7.08 -6.75 -10.72 -16.03 -14.45 -4.43 -11.80 -13.74 -18.73 -15.98 -10.80
Vacuole Periphery -3.06 3.31 3.42 9.61 2.32 -7.45 3.94 4.46 9.86 1.65 -3.19 3.51 3.64 12.15 1.88
Standard name
Human Ortholog
Description Phospholipid hydroperoxide glutathione peroxidase; induced by glucose starvation that protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress; GPX1 has a paralog, HYR1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (16%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Gpx1

Gpx1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gpx1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available