Standard name | |
---|---|
Human Ortholog | |
Description | Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lag1p; LAC1 has a paralog, LAG1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 4 | 9 | 3 | 0 | 0 | 5 | 2 | – | 3 | 2 | 2 | 4 | 10 | 9 | – | 3 | 4 | 0 | 3 | 5 | 1 | 11 | 6 |
Bud | 0 | 0 | 0 | 1 | 0 | 3 | 6 | 9 | – | 2 | 7 | 1 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 4 | 1 | 7 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 2 | 1 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 2 | 0 | 2 | 2 | 5 | 3 | – | 0 | 2 | 1 | 11 | 14 | 18 | – | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 8 | 25 | 11 | 11 | 29 | 29 | 21 | – | 22 | 19 | 21 | 25 | 34 | 33 | – | 22 | 39 | 0 | 2 | 3 | 0 | 2 | 4 |
Endoplasmic Reticulum | 63 | 72 | 189 | 29 | 48 | 26 | 72 | 19 | – | 44 | 7 | 21 | 217 | 244 | 271 | – | 3 | 6 | 100 | 101 | 442 | 154 | 405 | 314 |
Endosome | 1 | 0 | 11 | 13 | 16 | 15 | 15 | 18 | – | 4 | 4 | 8 | 0 | 0 | 0 | – | 0 | 6 | 0 | 1 | 1 | 1 | 9 | 11 |
Golgi | 5 | 0 | 3 | 1 | 4 | 0 | 2 | 0 | – | 0 | 0 | 0 | 0 | 4 | 0 | – | 2 | 10 | 2 | 1 | 2 | 0 | 6 | 2 |
Mitochondria | 2 | 3 | 35 | 34 | 19 | 53 | 65 | 76 | – | 108 | 88 | 82 | 0 | 0 | 0 | – | 11 | 10 | 0 | 1 | 4 | 3 | 10 | 12 |
Nucleus | 0 | 0 | 1 | 4 | 1 | 12 | 23 | 34 | – | 36 | 48 | 39 | 4 | 0 | 2 | – | 6 | 3 | 0 | 0 | 0 | 0 | 1 | 1 |
Nuclear Periphery | 16 | 9 | 87 | 46 | 139 | 114 | 254 | 163 | – | 218 | 91 | 178 | 49 | 13 | 16 | – | 35 | 36 | 9 | 17 | 41 | 60 | 142 | 78 |
Nucleolus | 4 | 1 | 0 | 4 | 6 | 10 | 12 | 51 | – | 26 | 9 | 12 | 0 | 0 | 0 | – | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1 | 0 | 3 | 1 | 2 | – | 0 | 0 | 1 | 0 | 1 | 0 | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 23 | 19 | 150 | 125 | 230 | 210 | 298 | 403 | – | 216 | 143 | 225 | 51 | 28 | 42 | – | 78 | 92 | 2 | 9 | 11 | 17 | 50 | 66 |
Unique Cell Count | 96 | 102 | 391 | 218 | 397 | 405 | 663 | 687 | 567 | 365 | 507 | 297 | 301 | 337 | 140 | 185 | 118 | 141 | 521 | 244 | 654 | 508 | ||
Labelled Cell Count | 118 | 117 | 512 | 272 | 476 | 477 | 787 | 801 | 681 | 422 | 592 | 361 | 348 | 391 | 164 | 212 | 118 | 141 | 521 | 244 | 654 | 508 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.1 | 10.9 | 9.5 | 9.6 | 9.7 | 8.6 | 8.9 | 9.0 | – | 8.5 | 8.7 | 8.8 | 12.0 | 12.3 | 12.0 | – | 9.5 | 11.7 | 13.2 | 13.0 | 13.2 |
Std Deviation (1e-4) | 2.4 | 2.1 | 2.1 | 2.4 | 2.2 | 1.9 | 1.9 | 2.0 | – | 1.8 | 2.0 | 2.0 | 2.0 | 2.3 | 2.1 | – | 2.7 | 2.9 | 2.4 | 2.8 | 2.8 |
Intensity Change (Log2) | – | – | – | 0.01 | 0.02 | -0.15 | -0.1 | -0.09 | – | -0.16 | -0.13 | -0.12 | 0.34 | 0.37 | 0.33 | – | -0.01 | 0.3 | 0.47 | 0.45 | 0.47 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | -2.1 | 0 | – | 0 | -2.0 | 0 | -0.9 | 0.8 | 0.3 | – | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 3.0 | 3.6 | 4.0 | – | 0 | 0 |
Cytoplasm | -0.7 | -2.4 | 0.4 | -1.4 | -2.6 | – | -1.8 | -0.7 | -1.5 | 1.0 | 2.3 | 1.7 | – | 3.3 | 5.2 |
Endoplasmic Reticulum | -8.6 | -11.1 | -13.3 | -13.6 | -18.2 | – | -14.4 | -14.6 | -15.5 | 6.5 | 8.8 | 8.9 | – | -9.8 | -10.7 |
Endosome | 1.9 | 0.9 | 0.7 | -0.6 | -0.2 | – | -2.6 | -1.7 | -1.3 | 0 | 0 | -3.1 | – | 0 | 0.3 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Mitochondria | 2.5 | -2.3 | 1.9 | 0.5 | 1.1 | – | 4.3 | 5.6 | 3.2 | -5.3 | -5.3 | -5.6 | – | -0.4 | -1.5 |
Nucleus | 0 | 0 | 3.0 | 3.4 | 4.2 | – | 4.8 | 7.2 | 5.4 | 0 | 0 | 0 | – | 0 | 0 |
Nuclear Periphery | -0.3 | 4.0 | 1.9 | 5.4 | 0.6 | – | 5.3 | 0.9 | 4.2 | -1.9 | -6.7 | -6.8 | – | 0.7 | -0.8 |
Nucleolus | 0 | 0 | 0 | 0 | 5.5 | – | 4.3 | 0 | 3.1 | 0 | 0 | 0 | – | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Vacuole | 4.5 | 5.5 | 3.8 | 2.1 | 6.4 | – | -0.1 | 0.2 | 1.8 | -6.1 | -8.7 | -7.9 | – | 3.6 | 2.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.6227 | 6.0979 | 6.2468 | 4.8562 | 5.476 | 5.7264 | 5.2869 | 6.5676 | 5.5684 | 5.7852 | 4.2707 | 4.9295 | 6.3065 | 6.2052 | 5.4099 | 4.6397 | 3.7748 | 5.314 |
Actin | 0.0007 | 0.0007 | 0.0006 | 0.0037 | 0.0002 | 0.0002 | 0.001 | 0.0003 | 0.0031 | 0.0002 | 0.0058 | 0.0002 | 0.0007 | 0.001 | 0.0002 | 0.0004 | 0.0005 | 0.0002 |
Bud | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0001 | 0.0004 | 0.0001 | 0.0024 | 0.0038 | 0.0003 | 0.0004 | 0 | 0.0003 | 0.0001 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0001 |
Bud Periphery | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0004 | 0 | 0.0004 | 0.0001 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 |
Bud Site | 0 | 0.0028 | 0.0002 | 0.003 | 0.0001 | 0 | 0.0006 | 0.0001 | 0.0058 | 0 | 0.0009 | 0 | 0 | 0.0002 | 0 | 0 | 0.0003 | 0 |
Cell Periphery | 0.0001 | 0.0003 | 0.0001 | 0.0004 | 0 | 0 | 0.0002 | 0.0004 | 0.0006 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0 |
Cytoplasm | 0.0127 | 0.061 | 0.0178 | 0.032 | 0.0266 | 0.0094 | 0.0058 | 0.001 | 0.0077 | 0.0016 | 0.0346 | 0.0108 | 0.0127 | 0.0025 | 0.0035 | 0.0056 | 0.0281 | 0.0014 |
Cytoplasmic Foci | 0.0008 | 0.0059 | 0.0019 | 0.0012 | 0.0356 | 0.0028 | 0.0024 | 0.008 | 0.0038 | 0.0001 | 0.0106 | 0.0003 | 0.0003 | 0.0033 | 0.0052 | 0.0006 | 0.0006 | 0.0001 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.3763 | 0.4509 | 0.5316 | 0.6618 | 0.4672 | 0.4438 | 0.5077 | 0.7326 | 0.6581 | 0.6816 | 0.5349 | 0.6054 | 0.5753 | 0.7705 | 0.7545 | 0.8541 | 0.7055 | 0.5562 |
Endosome | 0.0096 | 0.0081 | 0.0058 | 0.0094 | 0.0431 | 0.0086 | 0.0231 | 0.0012 | 0.0145 | 0.005 | 0.0365 | 0.0048 | 0.0058 | 0.0048 | 0.0019 | 0.0087 | 0.0085 | 0.0017 |
Golgi | 0.0003 | 0.0004 | 0.0003 | 0.0005 | 0.0031 | 0.0009 | 0.0014 | 0.0002 | 0.0106 | 0.0003 | 0.034 | 0.0001 | 0.0002 | 0.0018 | 0.0003 | 0.0006 | 0.0003 | 0.0001 |
Lipid Particles | 0.0026 | 0.0021 | 0.0027 | 0.0014 | 0.0046 | 0.0061 | 0.0104 | 0.0014 | 0.0116 | 0.0006 | 0.0119 | 0.0007 | 0.0015 | 0.0313 | 0.0125 | 0.0019 | 0.0008 | 0.0014 |
Mitochondria | 0.0005 | 0.0003 | 0.0004 | 0.0004 | 0.001 | 0.0014 | 0.001 | 0.0002 | 0.0087 | 0.0006 | 0.0076 | 0.0002 | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0001 |
None | 0.0222 | 0.0036 | 0.0114 | 0.0008 | 0.0011 | 0.0002 | 0.0009 | 0.0003 | 0.0018 | 0.0001 | 0.0126 | 0.0004 | 0.0015 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0001 |
Nuclear Periphery | 0.52 | 0.3816 | 0.3651 | 0.2579 | 0.3221 | 0.485 | 0.3885 | 0.1997 | 0.2342 | 0.2811 | 0.223 | 0.3587 | 0.3735 | 0.1644 | 0.2068 | 0.1171 | 0.2309 | 0.4237 |
Nucleolus | 0.0003 | 0.0016 | 0.0006 | 0.0001 | 0.0003 | 0.0001 | 0.0026 | 0.0002 | 0.0007 | 0 | 0.0012 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 |
Nucleus | 0.0387 | 0.0584 | 0.0341 | 0.0134 | 0.0551 | 0.0252 | 0.0108 | 0.0034 | 0.0063 | 0.0042 | 0.0103 | 0.0083 | 0.0076 | 0.0026 | 0.003 | 0.0021 | 0.0099 | 0.0054 |
Peroxisomes | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0043 | 0.0074 | 0.002 | 0.0001 | 0.0009 | 0 | 0.0017 | 0 | 0 | 0.0003 | 0 | 0 | 0 | 0 |
Punctate Nuclear | 0.0017 | 0.0077 | 0.0112 | 0.0027 | 0.0073 | 0.0012 | 0.0158 | 0.0431 | 0.0092 | 0.0001 | 0.0639 | 0.0004 | 0.0004 | 0.0056 | 0.0009 | 0.0006 | 0.0007 | 0.0002 |
Vacuole | 0.0045 | 0.0087 | 0.0061 | 0.003 | 0.0171 | 0.0022 | 0.0103 | 0.0028 | 0.0089 | 0.0056 | 0.0042 | 0.0035 | 0.0056 | 0.0052 | 0.0035 | 0.004 | 0.0071 | 0.002 |
Vacuole Periphery | 0.0087 | 0.0052 | 0.0095 | 0.0054 | 0.0069 | 0.0051 | 0.0145 | 0.005 | 0.0124 | 0.0184 | 0.0051 | 0.0057 | 0.0139 | 0.0059 | 0.0069 | 0.0036 | 0.005 | 0.0073 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 81.1972 | 65.9154 | 27.9497 | 40.5357 | 96.7399 | 50.0073 | 67.0437 | 50.8727 | 37.317 | 141.0793 |
Translational Efficiency | 1.2877 | 1.2237 | 0.9942 | 0.7621 | 1.1906 | 1.821 | 1.1984 | 0.8028 | 1.1469 | 0.9877 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
78 | 291 | 209 | 222 | 829 | 233 | 1611 | 706 | 907 | 524 | 1820 | 928 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1063.38 | 1224.80 | 1355.75 | 1136.58 | 928.42 | 1018.64 | 1220.38 | 1163.25 | 940.03 | 1133.13 | 1235.93 | 1156.87 |
Standard Deviation | 137.71 | 263.57 | 228.73 | 200.78 | 157.91 | 161.73 | 215.58 | 220.51 | 160.80 | 246.39 | 221.38 | 216.25 |
Intensity Change Log 2 | — | 0.203889 | 0.350434 | 0.096042 | — | 0.133795 | 0.394481 | 0.325312 | — | 0.171640 | 0.371133 | 0.207454 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.008102 | 0.000606 | 0.000228 | 0.000431 | 0.000381 | 0.000157 | 0.000967 | 0.000457 | 0.001045 | 0.000407 | 0.000882 | 0.000451 |
Bud Neck | 0.003466 | 0.001276 | 0.002302 | 0.003984 | 0.000443 | 0.000439 | 0.001451 | 0.002926 | 0.000703 | 0.000904 | 0.001549 | 0.003179 |
Bud Site | 0.006613 | 0.004224 | 0.001427 | 0.006942 | 0.000976 | 0.000876 | 0.001899 | 0.004074 | 0.001460 | 0.002735 | 0.001845 | 0.004760 |
Cell Periphery | 0.001424 | 0.000493 | 0.001127 | 0.000478 | 0.000441 | 0.000188 | 0.000345 | 0.000482 | 0.000525 | 0.000357 | 0.000434 | 0.000481 |
Cytoplasm | 0.085819 | 0.014564 | 0.002019 | 0.051615 | 0.008687 | 0.022165 | 0.028022 | 0.028108 | 0.015320 | 0.017944 | 0.025036 | 0.033731 |
Cytoplasmic Foci | 0.031481 | 0.004528 | 0.000428 | 0.013080 | 0.003083 | 0.006082 | 0.002648 | 0.005317 | 0.005526 | 0.005219 | 0.002393 | 0.007174 |
Eisosomes | 0.000241 | 0.000013 | 0.000037 | 0.000049 | 0.000025 | 0.000033 | 0.000035 | 0.000035 | 0.000044 | 0.000022 | 0.000035 | 0.000038 |
Endoplasmic Reticulum | 0.307024* | 0.385888* | 0.810049* | 0.643608* | 0.621523* | 0.751985* | 0.694065* | 0.760116* | 0.594477* | 0.548675* | 0.707384* | 0.732245* |
Endosome | 0.025520 | 0.031401 | 0.018859 | 0.025824 | 0.016304 | 0.011853 | 0.013342 | 0.023153 | 0.017096 | 0.022709 | 0.013975 | 0.023792 |
Golgi | 0.004067 | 0.001689 | 0.000327 | 0.000998 | 0.001719 | 0.001188 | 0.000690 | 0.002563 | 0.001921 | 0.001466 | 0.000648 | 0.002189 |
Lipid Particles | 0.038275 | 0.007115 | 0.002686 | 0.023527 | 0.010362 | 0.018289 | 0.004531 | 0.010087 | 0.012762 | 0.012084 | 0.004319 | 0.013302 |
Mitochondria | 0.000574 | 0.002327 | 0.000352 | 0.000370 | 0.003742 | 0.000660 | 0.000658 | 0.000939 | 0.003470 | 0.001586 | 0.000622 | 0.000803 |
Mitotic Spindle | 0.018163 | 0.008175 | 0.000361 | 0.015107 | 0.000717 | 0.000626 | 0.000933 | 0.006703 | 0.002217 | 0.004818 | 0.000867 | 0.008713 |
None | 0.000085 | 0.002643 | 0.000017 | 0.000284 | 0.002866 | 0.000394 | 0.000719 | 0.000431 | 0.002627 | 0.001643 | 0.000639 | 0.000396 |
Nuclear Periphery | 0.063825 | 0.199310* | 0.040409 | 0.020028 | 0.124846* | 0.041002 | 0.098994 | 0.024596 | 0.119598* | 0.128917* | 0.092266 | 0.023503 |
Nuclear Periphery Foci | 0.202268* | 0.314068* | 0.106728 | 0.124337 | 0.194437* | 0.132615 | 0.132245 | 0.103123 | 0.195111* | 0.233384* | 0.129315 | 0.108198 |
Nucleolus | 0.001088 | 0.004201 | 0.000014 | 0.000122 | 0.000451 | 0.000147 | 0.000106 | 0.000129 | 0.000506 | 0.002398 | 0.000096 | 0.000128 |
Nucleus | 0.027773 | 0.004889 | 0.000534 | 0.012024 | 0.003626 | 0.003949 | 0.007895 | 0.003914 | 0.005702 | 0.004471 | 0.007050 | 0.005854 |
Peroxisomes | 0.001810 | 0.000576 | 0.000470 | 0.000273 | 0.000628 | 0.002508 | 0.000284 | 0.000202 | 0.000729 | 0.001435 | 0.000305 | 0.000219 |
Vacuole | 0.171733 | 0.009320 | 0.011225 | 0.056180 | 0.003340 | 0.004400 | 0.009833 | 0.021238 | 0.017822 | 0.007132 | 0.009993 | 0.029597 |
Vacuole Periphery | 0.000648 | 0.002693 | 0.000403 | 0.000739 | 0.001402 | 0.000443 | 0.000339 | 0.001408 | 0.001338 | 0.001693 | 0.000346 | 0.001248 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.12 | 1.19 | 1.10 | -0.37 | -1.74 | 2.15 | -1.34 | -0.95 | -1.86 | 0.74 | 1.06 | 0.03 | 0.55 | -1.07 | 0.60 |
Bud Neck | 2.69 | 1.57 | 0.04 | -3.65 | -1.87 | -0.03 | -3.78 | -4.32 | -4.29 | -2.59 | -0.44 | -2.75 | -4.98 | -4.91 | -3.39 |
Bud Site | 1.37 | 2.55 | 0.58 | -0.92 | -2.38 | 0.16 | -2.11 | -3.51 | -3.45 | -2.63 | -0.74 | -0.52 | -3.62 | -1.92 | -3.37 |
Cell Periphery | 0.94 | 0.10 | 1.00 | 0.22 | 0.84 | 3.65 | 1.02 | -0.63 | -2.30 | -1.15 | 1.30 | 0.48 | -0.12 | -1.18 | -0.52 |
Cytoplasm | 4.23 | 5.08 | 1.47 | -4.20 | -5.80 | -2.65 | -7.82 | -6.46 | -2.19 | -1.54 | -0.58 | -3.42 | -5.89 | -4.69 | -3.74 |
Cytoplasmic Foci | 3.59 | 4.23 | 2.06 | -2.75 | -4.24 | -1.00 | 0.24 | -2.11 | -0.01 | -2.69 | 0.11 | 2.50 | -1.68 | -1.51 | -4.47 |
Eisosomes | 2.33 | 1.96 | 1.72 | -3.57 | -0.87 | -0.45 | -0.59 | -1.04 | -0.28 | -0.19 | 1.43 | 0.28 | -0.31 | -1.83 | -0.53 |
Endoplasmic Reticulum | -3.16 | -13.53 | -6.99 | -5.72 | 7.42 | -5.68 | -4.81 | -6.53 | 0.78 | -2.92 | 2.16 | -8.23 | -6.36 | -7.45 | 0.64 |
Endosome | -1.05 | 1.57 | 0.10 | 1.25 | -1.64 | 2.36 | 2.16 | -3.36 | -4.73 | -4.90 | -2.45 | 2.40 | -3.65 | -0.47 | -5.56 |
Golgi | 0.62 | 1.19 | 0.94 | 1.06 | -2.72 | 1.06 | 2.22 | -0.64 | -1.11 | -1.57 | 0.66 | 2.56 | -0.27 | -0.68 | -1.68 |
Lipid Particles | 5.18 | 6.00 | 1.69 | -3.66 | -4.64 | -1.52 | 3.18 | -0.26 | 1.43 | -3.73 | 0.18 | 4.81 | -0.70 | -0.73 | -5.60 |
Mitochondria | -3.47 | 0.91 | 0.97 | 3.91 | -0.19 | 3.02 | 3.00 | 2.62 | -0.85 | -0.80 | 1.92 | 3.03 | 2.76 | 2.09 | -0.66 |
Mitotic Spindle | 1.68 | 2.45 | 0.87 | -1.44 | -3.18 | 0.12 | -0.61 | -3.26 | -3.35 | -3.15 | -0.86 | 1.81 | -2.98 | -1.85 | -4.39 |
None | -2.80 | 3.02 | -1.64 | 2.37 | -2.02 | 2.85 | 2.42 | 2.76 | -0.52 | 1.02 | 0.99 | 2.45 | 2.73 | 2.26 | 0.82 |
Nuclear Periphery | -8.12 | 1.14 | 2.80 | 13.40 | 3.02 | 9.48 | 3.38 | 14.38 | 2.62 | 15.36 | -0.92 | 3.76 | 14.79 | 12.38 | 15.87 |
Nuclear Periphery Foci | -4.15 | 2.59 | 2.15 | 10.08 | -0.75 | 3.78 | 6.63 | 8.82 | 1.90 | 3.60 | -2.96 | 7.19 | 8.98 | 10.11 | 3.05 |
Nucleolus | -1.41 | 1.42 | 1.17 | 1.82 | -2.75 | 1.01 | 1.22 | 0.99 | -0.18 | -1.18 | -1.53 | 1.52 | 1.23 | 1.81 | -1.66 |
Nucleus | 3.48 | 4.25 | 1.78 | -2.39 | -3.74 | -0.20 | -3.17 | -2.32 | -1.86 | 0.46 | 0.68 | -1.16 | -2.01 | -2.76 | -1.21 |
Peroxisomes | 1.33 | 1.34 | 1.80 | 0.90 | 0.34 | -0.92 | 1.60 | 2.07 | 1.10 | 0.29 | -0.78 | 1.94 | 2.58 | 1.27 | 0.36 |
Vacuole | 7.61 | 7.51 | 3.61 | -6.28 | -6.09 | -0.83 | -5.80 | -7.47 | -7.11 | -5.21 | 4.32 | 3.20 | -4.65 | -8.59 | -7.82 |
Vacuole Periphery | -2.64 | 1.27 | -0.29 | 2.52 | -1.40 | 4.70 | 5.62 | 0.00 | -2.42 | -2.74 | -0.76 | 5.70 | 0.31 | 0.88 | -2.96 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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