Standard name | |
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Human Ortholog | |
Description | Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; role in misfolded protein quality control; forms a heterotrimeric complex with Erp1p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP2 has a paralog, ERP4, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 15 | 30 | 39 | 48 | 56 | 48 | 49 | 43 | 44 | 39 | 31 | 35 | 23 | 37 | 20 | 27 | 22 | 27 | 13 | 10 | 14 | 20 | 31 | 41 |
Bud | 3 | 2 | 8 | 3 | 8 | 10 | 20 | 17 | 16 | 16 | 23 | 18 | 1 | 3 | 2 | 2 | 2 | 0 | 0 | 2 | 0 | 1 | 2 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 12 | 5 | 6 | 8 | 10 | 19 | 24 | 19 | 30 | 28 | 34 | 30 | 19 | 52 | 59 | 42 | 15 | 26 | 1 | 0 | 1 | 1 | 2 | 2 |
Cytoplasm | 37 | 47 | 30 | 34 | 55 | 51 | 62 | 81 | 68 | 54 | 59 | 53 | 29 | 49 | 60 | 42 | 36 | 42 | 10 | 12 | 6 | 1 | 6 | 8 |
Endoplasmic Reticulum | 236 | 172 | 194 | 132 | 182 | 190 | 177 | 183 | 181 | 154 | 129 | 116 | 288 | 330 | 289 | 7 | 1 | 4 | 260 | 208 | 232 | 190 | 219 | 275 |
Endosome | 0 | 0 | 5 | 3 | 12 | 3 | 17 | 11 | 7 | 12 | 12 | 10 | 2 | 1 | 1 | 0 | 2 | 0 | 2 | 2 | 1 | 7 | 5 | 7 |
Golgi | 19 | 11 | 20 | 42 | 28 | 11 | 17 | 19 | 16 | 15 | 11 | 7 | 14 | 36 | 29 | 65 | 45 | 55 | 10 | 4 | 6 | 18 | 23 | 34 |
Mitochondria | 0 | 3 | 22 | 27 | 31 | 68 | 135 | 110 | 134 | 154 | 175 | 138 | 0 | 2 | 0 | 9 | 10 | 8 | 1 | 2 | 5 | 13 | 6 | 6 |
Nucleus | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 1 | 2 | 1 | 1 | 4 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 1 | 0 | 1 | 4 | 6 | 1 | 7 | 6 | 7 | 5 | 3 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 2 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 8 | 3 | 7 | 5 | 9 | 0 | 2 | 1 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 27 | 14 | 16 | 6 | 19 | 9 | 52 | 73 | 42 | 49 | 37 | 33 | 13 | 28 | 54 | 36 | 39 | 25 | 5 | 3 | 0 | 6 | 5 | 7 |
Unique Cell Count | 287 | 227 | 259 | 214 | 303 | 291 | 411 | 440 | 391 | 387 | 385 | 338 | 329 | 426 | 408 | 197 | 144 | 157 | 310 | 251 | 273 | 267 | 307 | 392 |
Labelled Cell Count | 350 | 284 | 341 | 303 | 406 | 415 | 561 | 568 | 550 | 536 | 527 | 458 | 392 | 542 | 517 | 236 | 178 | 189 | 310 | 251 | 273 | 267 | 307 | 392 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 16.5 | 17.5 | 12.6 | 12.4 | 11.3 | 10.8 | 9.8 | 10.0 | 9.9 | 9.3 | 9.9 | 9.6 | 18.1 | 19.1 | 18.5 | 22.3 | 20.3 | 22.2 | 18.9 | 17.2 | 18.0 |
Std Deviation (1e-4) | 3.6 | 3.9 | 3.0 | 3.1 | 2.5 | 2.3 | 2.5 | 2.5 | 2.0 | 2.6 | 2.5 | 2.3 | 4.3 | 4.9 | 5.0 | 6.1 | 7.2 | 6.0 | 6.4 | 5.5 | 4.3 |
Intensity Change (Log2) | – | – | – | -0.03 | -0.16 | -0.22 | -0.36 | -0.33 | -0.36 | -0.44 | -0.36 | -0.4 | 0.52 | 0.6 | 0.55 | 0.82 | 0.68 | 0.81 | 0.58 | 0.45 | 0.51 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 2.1 | 1.1 | 0.5 | -1.2 | -2.1 | -1.4 | -1.9 | -2.8 | -1.7 | -3.2 | -2.6 | -4.5 | -0.4 | 0.1 | 0.6 |
Bud | 0 | -0.3 | 0.2 | 1.1 | 0.5 | 0.7 | 0.7 | 1.7 | 1.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.9 | 0.7 | 2.4 | 2.1 | 1.4 | 2.9 | 2.7 | 3.4 | 3.3 | 2.1 | 4.5 | 5.2 | 6.6 | 3.5 | 5.3 |
Cytoplasm | 1.4 | 2.2 | 2.0 | 1.3 | 2.4 | 2.0 | 0.9 | 1.3 | 1.4 | -1.1 | 0 | 1.2 | 2.8 | 3.5 | 4.0 |
Endoplasmic Reticulum | -3.1 | -3.7 | -2.5 | -8.1 | -8.5 | -7.2 | -8.8 | -10.3 | -9.8 | 4.0 | 0.8 | -1.1 | -15.2 | -14.3 | -14.3 |
Endosome | 0 | 1.4 | 0 | 1.6 | 0.5 | 0 | 0.9 | 0.9 | 0.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 3.8 | 0.6 | -2.0 | -2.0 | -1.9 | -2.0 | -2.1 | -2.8 | -3.3 | -1.8 | 0.3 | -0.3 | 6.9 | 6.2 | 7.0 |
Mitochondria | 1.5 | 0.7 | 4.7 | 7.2 | 5.4 | 7.5 | 8.8 | 10.0 | 8.8 | -5.4 | -5.5 | -6.0 | -1.6 | -0.6 | -1.3 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.7 | 0 | -1.7 | 2.7 | 4.0 | 2.0 | 2.7 | 1.6 | 1.6 | -1.2 | 0.2 | 2.9 | 4.0 | 5.9 | 3.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 12.9008 | 15.6997 | 15.9987 | 15.0653 | 13.5652 | 14.506 | 14.899 | 18.7202 | 17.5534 | 17.5862 | 14.6366 | 17.5776 | 13.2118 | 16.8643 | 15.5403 | 16.2998 | 14.2694 | 15.4584 |
Actin | 0.0672 | 0.0223 | 0.0313 | 0.0003 | 0.0174 | 0.0042 | 0.045 | 0.0132 | 0.0262 | 0.0223 | 0.0286 | 0.02 | 0.0322 | 0.0075 | 0.0184 | 0.0029 | 0.0029 | 0.0057 |
Bud | 0.0008 | 0.0003 | 0.001 | 0 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 |
Bud Neck | 0.0014 | 0.0005 | 0.0011 | 0.0003 | 0.0009 | 0.0017 | 0.0008 | 0.0005 | 0.0013 | 0.0005 | 0.0002 | 0.0013 | 0.0005 | 0.0003 | 0.0009 | 0.0002 | 0.0004 | 0.0012 |
Bud Periphery | 0.0015 | 0.0009 | 0.0022 | 0 | 0.0006 | 0.0001 | 0.0004 | 0.0002 | 0.0007 | 0.0004 | 0.0001 | 0.0002 | 0.0005 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0003 |
Bud Site | 0.0032 | 0.0063 | 0.0016 | 0 | 0.0002 | 0.0001 | 0.002 | 0.0072 | 0.0067 | 0.0002 | 0.0001 | 0.0001 | 0.0009 | 0.0103 | 0.0015 | 0 | 0.0004 | 0 |
Cell Periphery | 0.0021 | 0.0036 | 0.002 | 0.0008 | 0.0014 | 0.0009 | 0.0016 | 0.0035 | 0.0035 | 0.0022 | 0.001 | 0.0016 | 0.0026 | 0.0037 | 0.002 | 0.002 | 0.001 | 0.0012 |
Cytoplasm | 0.0379 | 0.0753 | 0.0373 | 0.0456 | 0.0343 | 0.0291 | 0.0197 | 0.0348 | 0.0276 | 0.0053 | 0.0141 | 0.0174 | 0.0179 | 0.0289 | 0.0276 | 0.0293 | 0.0068 | 0.014 |
Cytoplasmic Foci | 0.029 | 0.0122 | 0.0306 | 0.0021 | 0.0068 | 0.0116 | 0.0303 | 0.0156 | 0.023 | 0.0048 | 0.0146 | 0.0145 | 0.0136 | 0.0132 | 0.0147 | 0.012 | 0.0188 | 0.0135 |
Eisosomes | 0.0004 | 0.0007 | 0.0003 | 0 | 0.0003 | 0.0001 | 0.0007 | 0.0002 | 0.0006 | 0.0003 | 0.0003 | 0.0003 | 0.0009 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0004 |
Endoplasmic Reticulum | 0.614 | 0.735 | 0.6532 | 0.8998 | 0.7477 | 0.748 | 0.5694 | 0.67 | 0.6539 | 0.8134 | 0.7211 | 0.6422 | 0.6976 | 0.7365 | 0.7252 | 0.8042 | 0.6532 | 0.7391 |
Endosome | 0.0528 | 0.0233 | 0.049 | 0.0104 | 0.0397 | 0.0416 | 0.0822 | 0.0401 | 0.055 | 0.0243 | 0.0598 | 0.0485 | 0.041 | 0.0281 | 0.0508 | 0.0283 | 0.0439 | 0.0403 |
Golgi | 0.0683 | 0.0448 | 0.1043 | 0.0129 | 0.0545 | 0.102 | 0.1465 | 0.0739 | 0.1437 | 0.0863 | 0.0939 | 0.2127 | 0.1085 | 0.0794 | 0.1033 | 0.1003 | 0.1694 | 0.1464 |
Lipid Particles | 0.0302 | 0.0399 | 0.0508 | 0.0185 | 0.0234 | 0.0416 | 0.0616 | 0.1284 | 0.0419 | 0.0302 | 0.0358 | 0.0303 | 0.0433 | 0.0768 | 0.0336 | 0.0135 | 0.0611 | 0.0274 |
Mitochondria | 0.0156 | 0.0048 | 0.02 | 0.0002 | 0.0284 | 0.0019 | 0.006 | 0.0007 | 0.004 | 0.0013 | 0.001 | 0.0044 | 0.0099 | 0.0005 | 0.002 | 0.0004 | 0.0276 | 0.002 |
None | 0.0043 | 0.0005 | 0.0007 | 0.0001 | 0.0029 | 0.0007 | 0.0021 | 0.0003 | 0.0007 | 0.0002 | 0.0004 | 0.0003 | 0.0062 | 0.0002 | 0.0005 | 0.0002 | 0.0003 | 0.0002 |
Nuclear Periphery | 0.0346 | 0.0177 | 0.0063 | 0.0061 | 0.0211 | 0.007 | 0.0132 | 0.0065 | 0.0044 | 0.0045 | 0.0089 | 0.003 | 0.0128 | 0.005 | 0.0107 | 0.0043 | 0.003 | 0.0047 |
Nucleolus | 0.0006 | 0.0002 | 0.0002 | 0 | 0.0004 | 0.0002 | 0.0007 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0.0001 | 0.0002 | 0 | 0.0003 | 0 |
Nucleus | 0.0107 | 0.0023 | 0.0008 | 0.0004 | 0.0024 | 0.0007 | 0.0011 | 0.0004 | 0.0003 | 0.0001 | 0.0005 | 0.0002 | 0.0012 | 0.0002 | 0.0015 | 0.0002 | 0.0002 | 0.0002 |
Peroxisomes | 0.0063 | 0.0003 | 0.0024 | 0 | 0.0054 | 0.0007 | 0.0043 | 0.0001 | 0.0013 | 0.0001 | 0.015 | 0.0003 | 0.0021 | 0.0003 | 0.0004 | 0.0002 | 0.0073 | 0.0004 |
Punctate Nuclear | 0.0058 | 0.0006 | 0.0007 | 0.0001 | 0.0058 | 0.0048 | 0.0075 | 0.0002 | 0.0003 | 0.0001 | 0.0004 | 0.0002 | 0.0029 | 0.0003 | 0.0012 | 0.0002 | 0.0004 | 0.0002 |
Vacuole | 0.0077 | 0.0067 | 0.0032 | 0.002 | 0.0046 | 0.0021 | 0.0029 | 0.0031 | 0.0029 | 0.0023 | 0.0031 | 0.0015 | 0.0028 | 0.0077 | 0.0032 | 0.0015 | 0.0017 | 0.0017 |
Vacuole Periphery | 0.0057 | 0.0019 | 0.0011 | 0.0005 | 0.0016 | 0.001 | 0.0016 | 0.0009 | 0.0014 | 0.001 | 0.0009 | 0.0009 | 0.002 | 0.0006 | 0.0013 | 0.0004 | 0.0009 | 0.0009 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 170.2785 | 135.4745 | 97.1393 | 100.9669 | 128.0486 | 110.6197 | 113.1224 | 111.6684 | 100.8552 | 142.9445 |
Translational Efficiency | 1.3142 | 1.319 | 0.9626 | 1.183 | 1.3116 | 1.9358 | 1.3844 | 1.119 | 1.0919 | 1.2412 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
236 | 1220 | 2368 | 127 | 1939 | 2189 | 1593 | 1315 | 2175 | 3409 | 3961 | 1442 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 928.92 | 1263.63 | 2019.21 | 2212.08 | 1275.09 | 1281.31 | 1718.45 | 1963.03 | 1237.53 | 1274.98 | 1898.25 | 1984.96 |
Standard Deviation | 156.99 | 280.99 | 378.84 | 486.70 | 217.97 | 231.97 | 354.73 | 525.89 | 237.96 | 250.76 | 397.69 | 527.30 |
Intensity Change Log 2 | — | 0.443948 | 1.120165 | 1.251777 | — | 0.007020 | 0.430509 | 0.622483 | — | 0.207501 | 0.762005 | 0.921683 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.004010 | 0.005424 | 0.002415 | 0.000544 | 0.002946 | 0.006975 | 0.002792 | 0.002920 | 0.003062 | 0.006420 | 0.002567 | 0.002710 |
Bud Neck | 0.006584 | 0.000468 | 0.000864 | 0.003444 | 0.000206 | 0.000619 | 0.000716 | 0.003919 | 0.000898 | 0.000565 | 0.000805 | 0.003877 |
Bud Site | 0.014248 | 0.008438 | 0.002390 | 0.013843 | 0.002763 | 0.006090 | 0.004765 | 0.020489 | 0.004009 | 0.006930 | 0.003345 | 0.019903 |
Cell Periphery | 0.005324 | 0.002372 | 0.003045 | 0.034302 | 0.001493 | 0.002882 | 0.003660 | 0.008022 | 0.001909 | 0.002700 | 0.003292 | 0.010337 |
Cytoplasm | 0.014717 | 0.011721 | 0.004135 | 0.001435 | 0.010628 | 0.011625 | 0.005493 | 0.026682 | 0.011071 | 0.011660 | 0.004681 | 0.024459 |
Cytoplasmic Foci | 0.041892 | 0.032673 | 0.010370 | 0.001296 | 0.039455 | 0.021955 | 0.012490 | 0.043483 | 0.039719 | 0.025790 | 0.011223 | 0.039767 |
Eisosomes | 0.000402 | 0.000200 | 0.000188 | 0.000242 | 0.000294 | 0.000556 | 0.000273 | 0.000140 | 0.000306 | 0.000429 | 0.000222 | 0.000149 |
Endoplasmic Reticulum | 0.813272* | 0.760149* | 0.638541* | 0.772887* | 0.763394* | 0.797735* | 0.557443* | 0.501036* | 0.768806* | 0.784284* | 0.605926* | 0.524978* |
Endosome | 0.034309 | 0.054207 | 0.048265 | 0.018364 | 0.038869 | 0.035392 | 0.057610 | 0.065529 | 0.038374 | 0.042125 | 0.052023 | 0.061375 |
Golgi | 0.025796 | 0.038912 | 0.086093 | 0.021835 | 0.058548 | 0.033073 | 0.089107 | 0.046219 | 0.054995 | 0.035163 | 0.087305 | 0.044072 |
Lipid Particles | 0.019693 | 0.053405 | 0.163125* | 0.089847 | 0.067918 | 0.066834 | 0.233009* | 0.158920* | 0.062685 | 0.062028 | 0.191230* | 0.152837* |
Mitochondria | 0.003804 | 0.003427 | 0.000229 | 0.000146 | 0.001042 | 0.001923 | 0.000223 | 0.001647 | 0.001342 | 0.002461 | 0.000226 | 0.001515 |
Mitotic Spindle | 0.000574 | 0.000588 | 0.000317 | 0.000511 | 0.000145 | 0.000218 | 0.000399 | 0.027893 | 0.000192 | 0.000350 | 0.000350 | 0.025481 |
None | 0.000288 | 0.002257 | 0.003598 | 0.000141 | 0.004027 | 0.003711 | 0.001998 | 0.002034 | 0.003622 | 0.003191 | 0.002955 | 0.001867 |
Nuclear Periphery | 0.000446 | 0.001068 | 0.000891 | 0.002308 | 0.000111 | 0.000135 | 0.000475 | 0.001494 | 0.000147 | 0.000469 | 0.000724 | 0.001566 |
Nuclear Periphery Foci | 0.005357 | 0.011237 | 0.016739 | 0.010424 | 0.002345 | 0.002881 | 0.012166 | 0.038222 | 0.002672 | 0.005871 | 0.014900 | 0.035773 |
Nucleolus | 0.000021 | 0.000063 | 0.000005 | 0.000011 | 0.000013 | 0.000028 | 0.000004 | 0.000428 | 0.000014 | 0.000040 | 0.000004 | 0.000391 |
Nucleus | 0.000424 | 0.000295 | 0.000095 | 0.000236 | 0.000121 | 0.000398 | 0.000150 | 0.001015 | 0.000153 | 0.000361 | 0.000117 | 0.000946 |
Peroxisomes | 0.002721 | 0.001020 | 0.000456 | 0.000071 | 0.002740 | 0.003969 | 0.000565 | 0.001260 | 0.002738 | 0.002914 | 0.000500 | 0.001155 |
Vacuole | 0.003313 | 0.007887 | 0.015684 | 0.024139 | 0.001496 | 0.001866 | 0.014650 | 0.044354 | 0.001693 | 0.004021 | 0.015268 | 0.042573 |
Vacuole Periphery | 0.002808 | 0.004188 | 0.002553 | 0.003974 | 0.001446 | 0.001139 | 0.002012 | 0.004296 | 0.001594 | 0.002230 | 0.002335 | 0.004268 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.80 | 1.06 | 2.45 | 4.17 | 2.96 | -3.58 | 0.08 | -0.23 | 3.51 | -0.35 | -3.68 | 0.70 | 0.24 | 4.02 | -0.48 |
Bud Neck | 1.55 | 1.44 | 0.73 | -2.00 | -1.71 | -3.05 | -6.81 | -7.65 | -6.75 | -6.69 | 0.75 | 0.16 | -5.09 | -7.19 | -6.68 |
Bud Site | 1.70 | 3.58 | 0.03 | -0.72 | -1.51 | -3.71 | -2.63 | -9.11 | -7.19 | -7.84 | -3.64 | 0.87 | -8.38 | -6.88 | -8.79 |
Cell Periphery | 1.66 | 1.31 | -4.45 | -5.06 | -4.96 | -5.03 | -11.49 | -9.66 | -7.13 | -6.28 | -2.60 | -6.07 | -9.66 | -8.68 | -8.22 |
Cytoplasm | 0.94 | 3.49 | 4.45 | 9.19 | 4.68 | -0.83 | 4.23 | -6.63 | -6.22 | -8.28 | -0.59 | 6.78 | -6.12 | -5.96 | -8.63 |
Cytoplasmic Foci | 1.31 | 4.71 | 6.08 | 12.97 | 11.34 | 6.80 | 11.41 | -1.46 | -7.25 | -10.88 | 5.82 | 13.45 | -0.32 | -5.27 | -11.30 |
Eisosomes | 1.94 | 2.13 | 1.07 | -0.41 | -0.55 | -4.28 | 0.64 | 5.03 | 7.08 | 4.36 | -2.76 | 3.09 | 5.05 | 6.69 | 3.19 |
Endoplasmic Reticulum | 2.67 | 9.16 | 1.35 | -0.42 | -5.09 | -3.75 | 18.79 | 20.99 | 24.27 | 4.89 | -1.85 | 19.65 | 20.56 | 22.92 | 7.67 |
Endosome | -3.57 | -2.78 | 2.72 | 9.24 | 9.62 | 1.69 | -8.55 | -9.20 | -10.64 | -2.68 | -2.00 | -8.01 | -8.49 | -7.31 | -3.72 |
Golgi | -2.73 | -12.16 | 0.64 | 3.31 | 12.14 | 8.42 | -7.09 | 3.45 | -3.85 | 9.93 | 7.25 | -9.99 | 3.34 | -2.78 | 13.03 |
Lipid Particles | -6.65 | -24.99 | -4.15 | -2.17 | 4.22 | 0.24 | -22.96 | -12.70 | -12.99 | 8.36 | 0.16 | -27.10 | -13.46 | -14.05 | 5.62 |
Mitochondria | 0.27 | 3.20 | 3.27 | 4.03 | 1.26 | -2.01 | 5.77 | -1.42 | 0.49 | -3.49 | -2.60 | 6.35 | -0.42 | 1.77 | -3.48 |
Mitotic Spindle | -0.08 | 0.83 | 0.14 | 0.32 | -1.09 | -1.47 | -2.68 | -9.36 | -9.33 | -9.27 | -2.48 | -2.61 | -9.33 | -9.27 | -9.26 |
None | -2.37 | -4.87 | 1.26 | 2.55 | 5.12 | 0.38 | 2.77 | 2.02 | 1.63 | -0.06 | 0.62 | 1.03 | 1.96 | 1.47 | 1.22 |
Nuclear Periphery | -0.94 | -2.35 | -4.64 | -1.72 | -3.85 | -1.14 | -12.07 | -5.77 | -5.67 | -4.26 | -1.41 | -12.32 | -6.37 | -3.58 | -3.75 |
Nuclear Periphery Foci | -3.05 | -6.75 | -1.52 | 0.19 | 1.96 | -0.90 | -9.40 | -12.34 | -11.97 | -8.59 | -4.63 | -14.09 | -12.37 | -10.96 | -7.51 |
Nucleolus | -1.05 | 2.61 | 1.35 | 1.30 | -1.58 | -1.17 | 1.33 | -2.10 | -2.02 | -2.14 | -1.58 | 1.55 | -2.09 | -1.94 | -2.14 |
Nucleus | 0.82 | 2.30 | 1.08 | 0.51 | -1.55 | -1.34 | -1.01 | -5.68 | -2.82 | -5.12 | -1.53 | 0.64 | -5.52 | -3.41 | -5.61 |
Peroxisomes | 2.48 | 3.39 | 3.98 | 5.29 | 4.48 | -1.95 | 5.79 | 2.30 | 3.64 | -1.52 | -0.37 | 6.82 | 2.72 | 2.96 | -1.61 |
Vacuole | -2.89 | -8.70 | -3.86 | -3.03 | -1.49 | -1.03 | -13.94 | -15.76 | -15.55 | -10.71 | -4.88 | -21.17 | -16.14 | -15.15 | -10.78 |
Vacuole Periphery | -1.46 | 0.37 | -0.80 | 0.09 | -0.98 | 1.48 | -3.00 | -4.81 | -5.37 | -3.84 | -1.94 | -4.37 | -4.81 | -3.30 | -3.45 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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