Standard name
Human Ortholog
Description Putative redox protein containing a thioredoxin fold; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0 0
Bud 0 0 0.06 0.05 0 0 – 0.05 0.07 0.05 0.08 0.07 0 0 0 0 0.05 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.11 0.1 0.12 0 0 0 0 0 0
Cytoplasm 0.66 0.49 0.5 0.36 0.33 0.14 – 0.14 0.07 0.1 0.13 0.07 0.8 0.79 0.82 0.12 0.35 0.07 0.27 0.18 0.1 0.28 0.27 0.28
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.19 0 – 0 0 0 0 0 0 0 0 0.2 0.1 0.19 0 0.05 0.08 0.07 0.06 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.19 0.2 0.05 0 0 0 0 0.08 0
Mitochondria 0.37 0.49 0.53 0.74 0.48 0.86 – 0.85 0.93 0.89 0.88 0.92 0.12 0.16 0.13 0.13 0.05 0.29 0.49 0.54 0.64 0.4 0.34 0.37
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.07 0 – 0 0 0 0 0 0 0 0 0 0 0.1 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0.07 0 0 0 0.06 0 0 0 0.06 0.05 0.12 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0.06 0 0 0.09 0.08 – 0 0.05 0.05 0 0 0.07 0.06 0 0.23 0.2 0.31 0.08 0.09 0 0.06 0.08 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 – 0 0 0 0 1 0 0 0 7 1 1 4 2 2 3 5 5
Bud 0 4 6 8 4 9 – 14 14 13 11 13 1 5 1 3 1 1 1 0 1 0 3 5
Bud Neck 0 2 2 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 1 2 1 0 4 3
Bud Site 0 0 1 1 2 1 – 5 8 4 2 9 0 0 0 1 0 0 – – – – – –
Cell Periphery 0 1 1 2 2 3 – 0 3 5 2 2 1 2 0 12 2 5 0 0 0 0 0 0
Cytoplasm 91 55 54 56 74 31 – 41 14 26 18 13 117 158 191 13 7 3 48 22 12 23 44 46
Endoplasmic Reticulum 4 4 0 0 0 0 – 0 2 1 0 2 2 0 0 6 0 1 3 0 1 0 1 2
Endosome 1 5 1 2 43 6 – 4 0 1 1 0 5 9 9 21 2 8 7 6 9 5 10 5
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 2 2 20 4 2 1 1 0 3 13 4
Mitochondria 51 55 57 114 107 188 – 254 191 242 122 161 17 32 30 14 1 12 86 69 75 33 56 61
Nucleus 1 1 0 1 1 2 – 4 4 8 3 2 0 1 1 3 1 1 0 0 0 0 0 1
Nuclear Periphery 1 1 1 1 1 0 – 7 3 2 5 2 0 0 0 0 0 0 0 0 1 0 1 0
Nucleolus 0 0 1 1 0 0 – 2 4 4 2 2 1 0 2 1 0 0 0 0 0 0 0 2
Peroxisomes 0 0 0 2 16 4 – 6 2 5 0 0 4 6 7 2 0 4 0 0 0 1 1 2
SpindlePole 2 0 4 5 8 5 – 20 6 9 4 10 0 0 1 6 1 5 3 2 1 2 2 1
Vac/Vac Membrane 9 7 2 6 20 17 – 8 10 13 2 1 10 12 10 24 4 13 14 11 3 5 12 14
Unique Cell Count 138 112 107 154 221 219 299 205 271 138 175 146 201 234 106 20 42 178 129 119 84 164 168
Labelled Cell Count 160 136 130 199 278 266 365 261 333 172 218 158 227 254 133 25 56 178 129 119 84 164 168


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 4.1 3.8 3.4 4.7 3.6 – 4.2 3.5 4.1 4.1 3.8 5.3 5.8 5.4 7.5 6.9 7.1 5.1 6.6 6.7
Std Deviation (1e-4) 0.4 1.2 1.9 1.4 2.5 1.1 – 2.5 1.8 2.1 3.2 1.8 2.6 3.4 3.1 1.9 1.9 1.2 2.6 3.9 4.1
Intensity Change (Log2) – – – -0.19 0.31 -0.09 – 0.15 -0.11 0.1 0.11 0.01 0.46 0.59 0.51 0.96 0.86 0.88 0.41 0.78 0.82


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 – –
Bud Neck – – – – – – – – – – – – 0 – –
Bud Site – – – – – – – – – – – – 0 – –
Cell Periphery – – – – – – – – – – – – 0 – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 – –
Endosome – – – – – – – – – – – – 0 – –
Golgi – – – – – – – – – – – – 0 – –
Mitochondria – – – – – – – – – – – – 0 – –
Nuclear Periphery – – – – – – – – – – – – 0 – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 – –
Peroxisomes – – – – – – – – – – – – 0 – –
SpindlePole – – – – – – – – – – – – 0 – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 – –
Cytoplasm – – – – – – – – – – – – 0 – –
Nucleus – – – – – – – – – – – – 0 – –
Vacuole – – – – – – – – – – – – 4.6 – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.0607 -0.0832 -0.0755 -0.206 0.5266 -0.156 -0.7347 -0.5097 -0.656 -0.3753 -0.7257 -0.5266 0.9503 0.8411 0.7639 0.7115 0.3315 0.9719
Actin 0.0186 0.0178 0.0214 0.0129 0.07 0.0154 0.0393 0.0116 0.0221 0.0437 0.008 0.0272 0.0691 0.0148 0.0249 0.0046 0.0285 0.0207
Bud 0.002 0.0074 0.0021 0.0034 0.011 0.001 0.0034 0.004 0.01 0.012 0.0014 0.0072 0.0033 0.0023 0.0023 0.0041 0.0025 0.0011
Bud Neck 0.0063 0.0043 0.0021 0.0025 0.0035 0.0096 0.0085 0.0048 0.0075 0.0021 0.0045 0.0065 0.0126 0.0011 0.0009 0.0011 0.0009 0.01
Bud Periphery 0.0041 0.0138 0.0039 0.0106 0.0119 0.0038 0.0082 0.0088 0.0169 0.0234 0.0019 0.0082 0.0069 0.0061 0.0056 0.0132 0.0051 0.0031
Bud Site 0.012 0.0721 0.0111 0.0024 0.0063 0.0015 0.0205 0.0486 0.0206 0.0079 0.0027 0.0032 0.0197 0.0383 0.0075 0.0034 0.0031 0.0017
Cell Periphery 0.0015 0.0086 0.0014 0.0012 0.002 0.0016 0.0025 0.0018 0.0035 0.0017 0.0003 0.0008 0.0025 0.0022 0.0013 0.0008 0.0005 0.001
Cytoplasm 0.0722 0.0982 0.0932 0.0854 0.0724 0.1142 0.1045 0.1473 0.1387 0.0967 0.2105 0.1126 0.0938 0.1327 0.0981 0.0895 0.0799 0.1306
Cytoplasmic Foci 0.1191 0.092 0.1326 0.1524 0.0918 0.1427 0.1361 0.1534 0.1355 0.1417 0.1755 0.1355 0.1213 0.093 0.0905 0.0926 0.1085 0.1102
Eisosomes 0.0011 0.0022 0.0011 0.0005 0.0004 0.0005 0.0011 0.0007 0.0012 0.0009 0.0004 0.0006 0.0012 0.0009 0.0007 0.0007 0.0003 0.0007
Endoplasmic Reticulum 0.0079 0.0042 0.0062 0.0044 0.0037 0.0042 0.0054 0.0044 0.0048 0.0074 0.0037 0.0031 0.0084 0.0148 0.0039 0.0039 0.0029 0.0039
Endosome 0.1317 0.0553 0.1001 0.1341 0.1135 0.1031 0.1276 0.1033 0.1015 0.0768 0.1156 0.1174 0.1102 0.0769 0.0736 0.0762 0.1335 0.1016
Golgi 0.0319 0.0191 0.0323 0.0641 0.0412 0.0543 0.0382 0.0425 0.0384 0.0171 0.0273 0.0576 0.0333 0.0203 0.027 0.0153 0.0421 0.0295
Lipid Particles 0.0462 0.014 0.0135 0.0035 0.0194 0.0277 0.0418 0.0105 0.0152 0.02 0.0225 0.0193 0.0497 0.0147 0.0155 0.0032 0.0105 0.0207
Mitochondria 0.1447 0.2207 0.2408 0.2732 0.2192 0.2647 0.1586 0.136 0.1659 0.2693 0.1003 0.2515 0.0924 0.1295 0.1892 0.3297 0.1929 0.2266
None 0.304 0.3244 0.2745 0.2018 0.2095 0.1635 0.2243 0.2682 0.2654 0.2162 0.2623 0.1788 0.3038 0.4127 0.4043 0.3319 0.3468 0.2825
Nuclear Periphery 0.0062 0.0012 0.0031 0.0014 0.0015 0.0014 0.0051 0.0018 0.0021 0.0056 0.0028 0.0035 0.0087 0.0018 0.0013 0.0012 0.0021 0.0039
Nucleolus 0.0035 0.0025 0.0011 0.0011 0.0045 0.0007 0.0027 0.0013 0.0014 0.0105 0.0017 0.0012 0.004 0.001 0.0007 0.0004 0.0004 0.0007
Nucleus 0.0032 0.0024 0.0068 0.0019 0.0049 0.0014 0.0024 0.0018 0.0026 0.0043 0.003 0.0022 0.0076 0.0016 0.0013 0.0008 0.0012 0.0017
Peroxisomes 0.0509 0.0228 0.0372 0.0136 0.0557 0.0778 0.0453 0.0367 0.027 0.0192 0.0346 0.0481 0.0285 0.0188 0.0381 0.0181 0.0218 0.0386
Punctate Nuclear 0.0068 0.0015 0.0042 0.0016 0.0038 0.0022 0.0045 0.0023 0.0039 0.0052 0.0114 0.0033 0.0074 0.0017 0.0014 0.0005 0.0011 0.0041
Vacuole 0.0213 0.0129 0.0082 0.0137 0.0411 0.0058 0.016 0.0084 0.0124 0.013 0.007 0.0079 0.0121 0.0118 0.0087 0.005 0.01 0.0046
Vacuole Periphery 0.0047 0.0025 0.0033 0.0144 0.0128 0.003 0.0042 0.0019 0.0033 0.0054 0.0026 0.0045 0.0035 0.003 0.0032 0.0039 0.0053 0.0024

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 53.5233 44.1661 30.9524 35.3864 39.1461 74.0682 52.099 38.5122 49.8206 34.8765
Translational Efficiency 1.7152 1.295 1.744 1.9886 1.232 1.4234 1.1631 2.3843 1.6016 1.685

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1260 545 399 698 23 1912 2375 1682 1283 2457 2774 2380

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 610.04 719.14 936.72 877.17 602.06 743.61 796.63 826.30 609.90 738.18 816.78 841.22
Standard Deviation 77.56 98.96 109.07 138.29 67.84 92.09 89.78 117.08 77.40 94.21 105.02 125.83
Intensity Change Log 2 — 0.237369 0.618714 0.523953 — 0.304639 0.404003 0.456758 — 0.271174 0.516054 0.490968

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000485 0.002049 0.001155 0.023023 0.000180 0.000529 0.000360 0.013330 0.000480 0.000866 0.000474 0.016173
Bud Neck 0.059458 0.050108 0.010038 0.026080 0.013910 0.064427 0.042834 0.034776 0.058642 0.061251 0.038116 0.032226
Bud Site 0.010693 0.035915 0.020450 0.142292* 0.004307 0.024147 0.020150 0.104503* 0.010578 0.026757 0.020193 0.115586*
Cell Periphery 0.000312 0.000320 0.000169 0.000843 0.000648 0.000279 0.001009 0.000364 0.000318 0.000288 0.000889 0.000505
Cytoplasm 0.220049* 0.147265* 0.401599* 0.123101* 0.013771 0.152974* 0.334238* 0.187713* 0.216351* 0.151708* 0.343927* 0.168764*
Cytoplasmic Foci 0.355198* 0.303563* 0.084722 0.125335 0.172533 0.285763* 0.060412 0.122121 0.351923* 0.289711* 0.063909 0.123064
Eisosomes 0.000528 0.000289 0.000076 0.000211 0.000239 0.000278 0.000392 0.000196 0.000523 0.000280 0.000346 0.000200
Endoplasmic Reticulum 0.001203 0.000753 0.004249 0.004313 0.000471 0.000598 0.001500 0.002093 0.001190 0.000633 0.001896 0.002744
Endosome 0.055035 0.072960 0.017537 0.054282 0.019133 0.079906 0.017429 0.053212 0.054392 0.078365 0.017445 0.053526
Golgi 0.016431 0.043629 0.004880 0.051012 0.045660 0.042541 0.002175 0.036733 0.016955 0.042782 0.002565 0.040921
Lipid Particles 0.037824 0.014738 0.004583 0.003766 0.040583 0.016039 0.009880 0.004163 0.037873 0.015750 0.009118 0.004047
Mitochondria 0.071831 0.114465* 0.001794 0.123714* 0.594134* 0.149838* 0.016304 0.104030* 0.081194 0.141992* 0.014217 0.109803*
Mitotic Spindle 0.001217 0.024352 0.025156 0.068016 0.000493 0.009603 0.017451 0.065388 0.001204 0.012875 0.018560 0.066159
None 0.002409 0.002274 0.002302 0.000834 0.000300 0.002263 0.006899 0.000991 0.002371 0.002265 0.006238 0.000945
Nuclear Periphery 0.000652 0.000261 0.001421 0.000498 0.000035 0.000370 0.001965 0.000693 0.000641 0.000346 0.001887 0.000636
Nuclear Periphery Foci 0.000736 0.000493 0.004157 0.000904 0.000049 0.000225 0.000731 0.000705 0.000723 0.000285 0.001224 0.000763
Nucleolus 0.002870 0.001967 0.001509 0.000919 0.000126 0.002094 0.006061 0.001544 0.002821 0.002066 0.005406 0.001360
Nucleus 0.068750 0.026177 0.278228* 0.039351 0.000561 0.042706 0.375394* 0.046029 0.067527 0.039040 0.361418* 0.044071
Peroxisomes 0.043618 0.094960 0.014735 0.083515 0.087690 0.053652 0.014077 0.097601 0.044408 0.062815 0.014172 0.093470
Vacuole 0.045469 0.054724 0.118716 0.116884 0.000735 0.063407 0.068470 0.115092 0.044667 0.061481 0.075697 0.115618
Vacuole Periphery 0.005232 0.008738 0.002524 0.011105 0.004443 0.008363 0.002267 0.008721 0.005218 0.008446 0.002304 0.009420

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.04 -2.56 -7.13 -6.50 -6.91 -3.92 -2.63 -9.47 -9.20 -9.32 -2.27 -0.02 -11.55 -11.23 -11.59
Bud Neck 1.78 13.16 7.86 4.77 -5.35 -4.19 -2.33 -1.70 8.82 2.55 -0.62 5.44 7.26 10.25 2.15
Bud Site -6.85 -2.27 -16.14 -11.59 -14.07 -5.00 -3.74 -16.93 -16.62 -17.47 -9.10 -4.66 -25.63 -20.52 -22.25
Cell Periphery -0.09 3.92 -2.13 -2.07 -2.84 1.63 -1.03 1.37 -1.09 4.08 0.74 -4.07 -1.87 -2.37 2.66
Cytoplasm 6.30 -10.71 8.14 0.81 15.50 -17.23 -35.98 -20.92 -6.20 15.42 8.48 -14.65 4.24 -4.74 21.42
Cytoplasmic Foci 4.77 32.22 28.09 16.16 -5.00 -2.09 2.16 1.09 26.96 -13.14 8.16 43.32 33.52 31.48 -14.49
Eisosomes 4.40 9.13 6.44 2.85 -9.22 -0.51 -1.45 0.67 3.02 3.50 4.68 3.24 6.74 3.67 3.03
Endoplasmic Reticulum 1.72 -8.38 -7.08 -7.93 0.20 -0.35 -2.98 -3.60 -5.48 -1.76 3.21 -4.86 -6.04 -9.13 -3.09
Endosome -3.00 11.53 1.72 4.10 -7.81 -7.23 0.27 -3.52 8.77 -12.02 -6.49 12.88 1.94 9.77 -14.18
Golgi -6.82 6.34 -6.43 -0.01 -9.22 0.30 3.74 1.17 3.48 -14.08 -11.69 11.86 -8.16 2.79 -17.19
Lipid Particles 8.20 14.75 15.47 5.71 0.53 1.12 1.42 1.68 10.57 5.47 9.44 12.65 15.57 12.08 5.63
Mitochondria -4.14 14.88 -3.27 0.69 -12.00 5.38 7.02 6.09 8.27 -15.72 -8.72 12.69 -2.10 7.55 -19.05
Mitotic Spindle -5.71 -4.04 -10.56 -4.99 -5.42 -7.89 -9.48 -16.03 -13.08 -10.56 -9.06 -9.91 -19.02 -14.11 -12.23
None 0.11 0.82 3.08 2.41 3.44 -3.63 -8.53 -3.48 2.30 7.49 0.19 -4.66 3.28 2.93 7.76
Nuclear Periphery 3.81 -6.62 -0.38 -3.49 5.73 -5.56 -14.21 -7.15 -3.63 7.34 2.95 -9.48 -1.23 -4.52 8.57
Nuclear Periphery Foci 0.17 -4.78 -0.94 -2.60 4.96 -3.66 -4.60 -3.48 -2.38 0.23 0.92 -1.35 -0.30 -3.38 2.13
Nucleolus 1.16 2.12 3.03 2.59 1.45 -9.23 -8.63 -4.95 1.63 6.01 1.27 -2.88 2.42 2.64 6.36
Nucleus 8.62 -16.77 1.93 -4.47 16.88 -17.46 -61.25* -18.49 -3.39 46.79* 7.22 -45.74 2.70 -4.93 49.71
Peroxisomes -6.92 8.25 -4.89 2.18 -9.91 1.22 2.64 -0.02 -7.13 -16.72 -5.01 10.61 -9.20 -5.27 -19.44
Vacuole -2.60 -11.52 -13.27 -10.50 -0.66 -24.78 -26.80 -30.09 -14.69 -11.80 -5.65 -11.95 -21.72 -18.04 -11.91
Vacuole Periphery -2.49 3.35 -2.63 -0.44 -4.37 -2.10 1.30 -1.69 0.80 -7.98 -4.51 5.46 -3.58 0.27 -9.00
Standard name
Human Ortholog
Description Putative redox protein containing a thioredoxin fold; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
Localization
Cell Percentages mitochondrion (37%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Fmp46

Fmp46


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fmp46-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available