Standard name
Human Ortholog
Description Glycerol-3-phosphate/dihydroxyacetone phosphate sn-1 acyltransferase; located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0.07 0 0 0 0 0 0.06 0.06 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.05 0.07 0 0 0 0 0.05 0.08 0.07 0 0.11 0.14 0 0 0.07 0 0 0 0 0 0
Cytoplasm 0.28 0.4 0.29 0.41 0.52 0.62 0.64 0.58 0.6 0.58 0.56 0.53 0.39 0.25 0.23 0.78 0.73 0.76 0 0 0 0 0 0
Endoplasmic Reticulum 0.59 0.63 0.7 0.55 0.41 0.29 0.28 0.28 0.29 0.29 0.34 0.25 0.67 0.67 0.69 0 0 0 0.82 0.78 0.88 0.82 0.84 0.82
Endosome 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.19 0 0.07 0 0.12 0.1 0.07 0.09 0.07 0.06 0 0.11 0 0.09 0.07 0.11 0.18 0.14 0.15 0.1 0.07 0.09 0.06 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 1 1 13 3 6 8 20 7 16 12 20 0 2 2 5 3 2 0 1 0 0 1 2
Bud 0 2 1 2 2 3 12 11 7 8 3 14 0 1 0 1 0 0 0 1 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 2 0 2 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 1 8 17 8 11 14 8 13 16 20 0 18 26 6 2 6 0 0 0 0 0 0
Cytoplasm 86 100 67 72 137 169 196 267 120 151 114 145 7 41 43 118 85 67 3 11 1 2 7 8
Endoplasmic Reticulum 180 157 163 96 107 79 87 130 59 76 69 68 12 109 131 2 1 3 288 227 230 88 162 184
Endosome 2 0 1 1 2 2 14 7 2 1 0 2 0 0 0 4 3 1 1 5 1 1 0 1
Golgi 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 2 0 1 0 2
Mitochondria 0 4 0 3 0 0 1 7 2 9 3 16 0 0 0 4 4 0 0 4 0 1 4 1
Nucleus 1 0 0 0 0 1 1 1 1 1 0 0 0 1 1 2 5 3 0 0 0 0 0 0
Nuclear Periphery 2 3 1 2 3 0 0 7 2 5 0 3 0 0 0 0 1 1 1 3 2 0 1 0
Nucleolus 0 0 0 0 0 1 0 1 0 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 57 7 17 5 32 27 21 40 15 17 8 30 0 15 14 17 21 12 51 30 19 9 12 18
Unique Cell Count 304 248 232 174 261 273 307 458 200 262 205 273 18 163 190 151 116 88 351 292 261 108 195 225
Labelled Cell Count 334 274 252 202 303 296 351 507 223 300 225 319 19 188 217 160 125 95 351 292 261 108 195 225


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.2 10.0 9.8 9.8 11.0 9.6 9.6 9.1 8.9 8.6 8.8 8.7 8.3 11.4 11.2 10.4 10.5 14.5 9.6 11.3 12.0
Std Deviation (1e-4) 1.4 1.5 1.5 1.9 1.8 1.6 1.9 1.7 2.1 1.7 1.9 1.9 1.8 1.7 1.8 1.7 2.8 2.7 1.3 2.2 1.7
Intensity Change (Log2) – – – -0.01 0.17 -0.02 -0.03 -0.11 -0.14 -0.19 -0.15 -0.18 -0.24 0.22 0.19 0.08 0.1 0.56 -0.03 0.2 0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 3.8 0 0 0 2.8 0 3.5 3.3 3.9 0 0 0 0 0 0
Bud 0 0 0 2.6 0 0 0 0 3.1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 3.6 0 2.5 2.2 0 3.0 4.0 3.9 0 4.9 5.5 0 0 0
Cytoplasm 2.6 5.3 7.4 8.0 7.3 6.5 6.4 5.7 5.5 0.9 -0.8 -1.5 9.4 7.9 7.7
Endoplasmic Reticulum -3.1 -6.5 -9.3 -9.7 -10.5 -8.5 -9.2 -7.7 -10.2 -0.3 -0.7 -0.3 -13.3 -12.2 -10.7
Endosome 0 0 0 2.9 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 3.7 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.0 1.8 1.0 -0.2 0.6 0.1 -0.4 -1.5 1.4 0 0.7 0 1.3 3.0 1.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.6869 7.9073 7.7409 7.2341 6.9056 7.6015 7.3865 8.2668 6.7702 6.5267 6.7633 7.2943 9.6131 9.0057 8.8423 8.6458 8.7732 8.895
Actin 0.0006 0.0002 0.0001 0.0018 0.0002 0.0001 0.0208 0.0005 0.0082 0.0002 0.0077 0.0077 0 0 0.0005 0.0003 0.0005 0.0004
Bud 0.0001 0 0 0.0001 0 0 0 0 0 0 0.0238 0 0 0 0 0 0 0
Bud Neck 0.0002 0.0014 0.0003 0.0016 0.0009 0.0007 0.0003 0.0002 0.0003 0.0001 0.0004 0.0007 0 0.003 0.0007 0.0002 0.0005 0.0004
Bud Periphery 0.0001 0.0001 0 0.0002 0.0001 0 0 0.0001 0.0001 0 0.0125 0.0001 0 0.0001 0.0001 0 0 0
Bud Site 0.0002 0.0005 0.0002 0.0002 0.0001 0 0.0006 0.0005 0.0004 0.0001 0.0011 0.0001 0.0001 0.0004 0.0002 0.0001 0 0
Cell Periphery 0.0004 0.0057 0.0011 0.0027 0.0014 0.0009 0.0002 0.0013 0.0014 0.0006 0.0006 0.0009 0.0004 0.0018 0.0013 0.0005 0.0023 0.0005
Cytoplasm 0.0557 0.0697 0.0458 0.0403 0.0612 0.0418 0.0523 0.0735 0.0578 0.0512 0.1298 0.0686 0.0242 0.0576 0.0521 0.0265 0.0732 0.042
Cytoplasmic Foci 0.0017 0.0024 0.0012 0.0005 0.0021 0.0011 0.0155 0.0027 0.006 0.0025 0.004 0.0013 0.0005 0.0013 0.0019 0.0009 0.0099 0.0011
Eisosomes 0 0 0 0 0 0 0.0002 0 0.0002 0 0.0003 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.8706 0.7918 0.901 0.8455 0.8427 0.8925 0.8517 0.8247 0.8525 0.8887 0.7329 0.8536 0.9575 0.8805 0.9048 0.9515 0.8516 0.9247
Endosome 0.0119 0.0197 0.0038 0.0038 0.0138 0.0097 0.0145 0.016 0.008 0.0131 0.0134 0.0057 0.0016 0.0062 0.0071 0.0047 0.0184 0.0059
Golgi 0.0012 0.0005 0.0003 0.0004 0.0005 0.0002 0.002 0.0004 0.0035 0.0006 0.0048 0.0009 0.0001 0.0003 0.0019 0.001 0.0011 0.0003
Lipid Particles 0.0021 0.0122 0.0099 0.001 0.0061 0.0025 0.0117 0.0098 0.0173 0.0059 0.0079 0.0039 0.004 0.0082 0.0081 0.0031 0.0157 0.0019
Mitochondria 0.0004 0.0002 0 0.0002 0.0001 0.0001 0.0002 0.0001 0.0026 0.0001 0.0211 0.0004 0 0.0001 0.0002 0.0001 0.0001 0.0001
None 0.0006 0.0015 0.0002 0.0001 0.0009 0.0002 0.0044 0.0003 0.015 0.0006 0.0139 0.0105 0.001 0.0011 0.0014 0.0011 0.0005 0.0008
Nuclear Periphery 0.0358 0.0344 0.0097 0.041 0.0295 0.0257 0.0079 0.0208 0.0081 0.0192 0.0081 0.017 0.0065 0.0094 0.0068 0.0053 0.0087 0.0087
Nucleolus 0.0001 0.0001 0.0036 0 0.0001 0 0.0001 0.0001 0.0001 0 0.0002 0 0 0.0001 0.0001 0 0 0
Nucleus 0.0057 0.0028 0.0013 0.003 0.0054 0.0033 0.0008 0.0092 0.0009 0.001 0.0018 0.0027 0.0003 0.002 0.0013 0.0004 0.0008 0.0014
Peroxisomes 0.0001 0 0.0001 0 0 0 0.0027 0 0.0054 0 0.0018 0.0004 0 0 0 0 0.0001 0
Punctate Nuclear 0.0004 0.0005 0.0002 0.0001 0.0006 0.0002 0.011 0.0009 0.0013 0.0003 0.0007 0.0002 0.0001 0.0003 0.0006 0.0001 0.0004 0.0001
Vacuole 0.0108 0.054 0.0205 0.0559 0.0332 0.0201 0.0028 0.0377 0.0097 0.0148 0.0125 0.0244 0.0034 0.0272 0.0105 0.0038 0.0156 0.0113
Vacuole Periphery 0.0014 0.0022 0.0006 0.0017 0.0011 0.0009 0.0003 0.0009 0.0012 0.001 0.0009 0.0008 0.0001 0.0004 0.0003 0.0002 0.0006 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.5722 7.5127 12.7892 27.5586 16.3444 20.7515 27.0332 30.9119 37.0729 27.6492
Translational Efficiency 0.7994 1.3173 1.1388 0.813 0.7588 0.9221 0.7094 0.8943 0.9042 0.8844

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
993 1405 304 1062 2336 2110 3219 1330 3329 3515 3523 2392

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1000.91 1069.96 1340.05 1161.79 959.16 1065.18 1140.54 1042.00 971.61 1067.09 1157.76 1095.18
Standard Deviation 151.80 192.32 178.58 151.23 177.86 156.03 152.20 140.79 171.57 171.48 164.49 157.22
Intensity Change Log 2 — 0.096245 0.420975 0.215037 — 0.151254 0.249874 0.119512 — 0.123425 0.339778 0.169082

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002864 0.000277 0.000380 0.000978 0.000096 0.000180 0.000140 0.000549 0.000922 0.000218 0.000161 0.000740
Bud Neck 0.004313 0.000603 0.001381 0.001268 0.001342 0.000830 0.001379 0.003109 0.002228 0.000739 0.001379 0.002291
Bud Site 0.016413 0.003638 0.001392 0.002653 0.001876 0.002280 0.001495 0.003791 0.006213 0.002823 0.001486 0.003285
Cell Periphery 0.001334 0.000815 0.000840 0.000897 0.000824 0.000776 0.000656 0.001019 0.000976 0.000792 0.000672 0.000965
Cytoplasm 0.109579* 0.056681 0.029454 0.026329 0.075281 0.046384 0.090990 0.102717* 0.085512 0.050500 0.085680 0.068802
Cytoplasmic Foci 0.030263 0.014070 0.005733 0.001897 0.021729 0.007814 0.002025 0.017271 0.024274 0.010315 0.002345 0.010445
Eisosomes 0.000286 0.000054 0.000066 0.000091 0.000107 0.000079 0.000100 0.000167 0.000161 0.000069 0.000097 0.000133
Endoplasmic Reticulum 0.538469* 0.845968* 0.758087* 0.923032* 0.797971* 0.858212* 0.839761* 0.726095* 0.720565* 0.853318* 0.832713* 0.813531*
Endosome 0.026305 0.010890 0.017556 0.003525 0.011294 0.007438 0.003720 0.012745 0.015771 0.008818 0.004914 0.008651
Golgi 0.009437 0.004331 0.000595 0.000565 0.004050 0.003420 0.000770 0.002038 0.005657 0.003784 0.000755 0.001384
Lipid Particles 0.021919 0.008585 0.009173 0.004203 0.018416 0.006178 0.005249 0.019683 0.019461 0.007141 0.005588 0.012810
Mitochondria 0.006383 0.001006 0.000053 0.000161 0.002028 0.002284 0.001154 0.001070 0.003327 0.001773 0.001059 0.000667
Mitotic Spindle 0.006609 0.000089 0.000265 0.000093 0.000086 0.000111 0.000143 0.001225 0.002032 0.000102 0.000154 0.000722
None 0.001067 0.002666 0.000071 0.000299 0.002639 0.000619 0.002095 0.000934 0.002170 0.001437 0.001920 0.000652
Nuclear Periphery 0.011257 0.002484 0.023196 0.004222 0.004942 0.004393 0.009095 0.014563 0.006826 0.003630 0.010312 0.009972
Nuclear Periphery Foci 0.032262 0.008754 0.075754 0.017109 0.007247 0.003805 0.011177 0.027037 0.014709 0.005783 0.016749 0.022629
Nucleolus 0.000180 0.000573 0.000035 0.000010 0.000821 0.000136 0.000074 0.000335 0.000630 0.000311 0.000071 0.000191
Nucleus 0.017910 0.002793 0.002707 0.002184 0.011794 0.004037 0.016920 0.021204 0.013619 0.003540 0.015693 0.012759
Peroxisomes 0.001037 0.000847 0.000065 0.000072 0.000626 0.000426 0.000154 0.000612 0.000749 0.000594 0.000147 0.000372
Vacuole 0.156708 0.031479 0.072500 0.010320 0.034530 0.047685 0.012664 0.043278 0.070974 0.041207 0.017828 0.028645
Vacuole Periphery 0.005405 0.003395 0.000695 0.000093 0.002300 0.002911 0.000238 0.000558 0.003226 0.003105 0.000277 0.000352

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 3.22 3.03 2.16 -1.75 -1.35 -1.31 -2.05 -3.26 -2.47 -2.86 2.90 3.12 0.67 -2.66 -2.95
Bud Neck 4.38 3.36 3.05 -1.40 0.18 1.80 -0.15 -3.11 -3.98 -2.82 4.70 2.01 -0.37 -4.04 -2.06
Bud Site 5.77 7.13 6.35 0.79 -1.48 -0.59 0.79 -2.42 -1.72 -2.85 4.24 6.62 3.46 -0.79 -3.07
Cell Periphery 2.67 1.81 1.71 -0.46 -0.22 0.30 1.01 -0.87 -1.18 -1.69 1.47 2.13 -0.02 -1.24 -1.85
Cytoplasm 8.68 11.13 14.46 7.50 0.72 8.30 -4.43 -5.06 -10.60 -2.17 11.42 -0.04 4.29 -5.30 4.41
Cytoplasmic Foci 6.24 9.21 12.62 7.90 2.51 8.02 12.55 1.70 -5.65 -9.72 9.52 16.88 8.62 -0.46 -8.69
Eisosomes 4.07 3.28 3.15 -1.41 -0.57 1.73 0.35 -2.64 -3.58 -2.54 4.55 2.67 1.08 -3.55 -1.66
Endoplasmic Reticulum -22.60* -11.14 -29.46 -8.69 -9.77 -7.46 -5.33 6.69 12.59 11.15 -18.50 -15.52 -10.82 5.83 2.80
Endosome 7.61 3.10 12.10 8.34 6.79 4.42 9.65 -1.34 -5.05 -9.01 7.90 13.13 7.29 0.02 -5.91
Golgi 2.81 5.39 5.41 6.05 0.23 0.85 7.19 3.90 2.19 -4.51 2.49 8.39 7.13 5.32 -3.60
Lipid Particles 8.12 6.15 11.51 3.80 3.35 8.40 9.10 -0.78 -8.77 -9.41 10.82 12.06 4.78 -5.84 -7.23
Mitochondria 2.91 3.48 3.42 3.52 -2.19 -0.46 3.54 1.87 1.76 0.17 2.36 3.95 4.30 2.70 1.39
Mitotic Spindle 3.63 3.52 3.62 -0.48 1.77 -0.32 -0.90 -1.88 -1.84 -1.77 3.58 3.45 1.83 -1.86 -1.68
None -1.42 1.47 1.10 2.49 -1.33 4.09 0.94 3.24 -1.29 2.89 1.29 0.51 3.64 1.95 3.72
Nuclear Periphery 5.31 -3.19 4.21 -2.12 5.35 0.68 -4.34 -5.09 -5.44 -2.82 4.21 -3.67 -2.64 -5.72 0.29
Nuclear Periphery Foci 8.76 -5.65 4.98 -4.16 7.83 3.64 -3.51 -9.24 -11.68 -7.62 8.21 -1.45 -4.79 -11.86 -3.64
Nucleolus -1.52 3.87 5.62 2.14 1.32 4.58 5.16 2.62 -1.89 -2.58 2.01 5.46 3.66 0.97 -2.13
Nucleus 9.49 9.13 9.62 0.84 0.82 7.01 -3.51 -4.66 -9.60 -2.07 11.13 -1.62 0.62 -8.76 2.08
Peroxisomes 0.43 5.16 5.11 2.31 -0.38 2.59 7.95 -0.13 -2.06 -5.31 0.94 8.37 4.35 1.43 -4.33
Vacuole 16.67 8.45 19.85 7.23 7.95 -4.07 10.34 -2.89 0.63 -9.80 9.71 18.18 13.00 4.27 -5.27
Vacuole Periphery 1.48 4.10 4.67 5.84 5.24 -1.60 10.20 7.62 6.77 -2.68 0.15 8.07 7.78 8.97 -1.01
Standard name
Human Ortholog
Description Glycerol-3-phosphate/dihydroxyacetone phosphate sn-1 acyltransferase; located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen
Localization
Cell Percentages ER (97%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Gpt2

Gpt2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gpt2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available