ORF
Human Ortholog
Description Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.07 0 0 0 0.05 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Bud Neck 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0 0 0 0 0 0 0
Cytoplasm 0.63 0.72 0.79 0.7 0.79 0.54 0.5 0.49 0.43 0.39 0.38 0.38 0.91 0.91 0.88 0.22 0.2 0.14 0.3 0.33 0.24 0.37 0.36 0.47
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0.12 0.28 0.29 0.06 0.06 0.05 0 0.05 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.37 0.22 0.08 0.12 0.11 0.42 0.51 0.46 0.62 0.63 0.7 0.58 0 0 0 0.1 0.08 0.11 0.45 0.44 0.5 0.39 0.38 0.28
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.16 0.33 0 0 0 0 0 0
SpindlePole 0 0 0.09 0.12 0.08 0.07 0.07 0.1 0.05 0.05 0.05 0.09 0 0 0 0.34 0.24 0.24 0 0 0 0 0.08 0.05
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.15 0.16 0.15 0 0.08 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 4 0 0 0 1 0 0 0 0 1 0 1 0 7 1 1 6 3 5 4 3 3
Bud 2 2 3 4 6 5 21 9 13 13 17 8 2 0 0 4 7 14 4 1 2 2 2 4
Bud Neck 2 0 12 7 9 5 5 6 4 6 2 1 4 1 1 1 1 2 1 3 2 1 1 9
Bud Site 1 0 0 0 0 0 5 3 1 7 3 2 0 0 0 1 1 3 – – – – – –
Cell Periphery 0 1 0 0 0 1 0 0 2 4 1 1 0 0 1 21 18 9 2 0 3 0 1 1
Cytoplasm 185 86 147 111 174 115 147 132 125 127 121 102 164 192 203 65 45 31 91 52 51 55 91 104
Endoplasmic Reticulum 4 5 0 0 0 1 0 2 0 2 0 1 0 1 4 12 7 6 5 1 1 0 1 0
Endosome 3 0 1 9 3 6 0 4 2 0 0 4 2 5 7 35 62 66 18 10 10 3 12 10
Golgi 1 0 2 1 0 0 0 0 0 0 0 0 0 0 0 11 7 1 2 0 1 0 2 2
Mitochondria 108 26 15 19 24 90 151 124 180 205 226 153 8 5 1 29 18 25 138 70 107 57 97 61
Nucleus 0 0 0 0 1 1 8 7 2 6 2 3 0 0 0 8 4 5 0 1 0 0 0 0
Nuclear Periphery 4 1 0 0 1 1 2 2 0 2 0 0 0 0 1 0 2 0 1 0 1 1 0 0
Nucleolus 1 0 0 2 0 1 1 0 2 0 0 0 0 0 0 1 4 0 0 0 0 1 1 0
Peroxisomes 0 0 3 1 3 3 0 3 0 0 0 1 2 2 9 34 36 75 3 0 2 1 4 1
SpindlePole 9 0 16 19 17 14 21 26 15 17 15 23 7 6 6 101 53 55 13 0 7 4 19 10
Vac/Vac Membrane 6 4 0 2 7 8 8 5 4 9 8 7 3 2 2 45 36 35 7 12 4 5 9 8
Unique Cell Count 293 119 187 158 221 214 294 267 289 326 321 266 181 210 230 295 224 226 308 160 213 147 256 223
Labelled Cell Count 326 126 203 175 245 251 370 323 350 398 395 307 192 215 235 375 302 328 308 160 213 147 256 223


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.1 4.3 4.5 4.6 4.6 3.8 3.4 4.4 3.5 3.6 3.3 3.7 4.5 5.0 5.1 6.3 6.0 5.5 4.5 4.5 5.1
Std Deviation (1e-4) 0.6 0.7 1.3 2.1 2.1 1.4 1.3 1.9 1.5 1.5 1.6 1.2 0.9 1.6 1.4 1.3 1.7 1.3 1.2 1.3 1.7
Intensity Change (Log2) – – – 0.01 0.01 -0.25 -0.42 -0.05 -0.39 -0.34 -0.47 -0.29 -0.0 0.13 0.18 0.47 0.4 0.28 -0.01 -0.01 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 5.6 5.7 5.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.6359 0.5824 0.5281 0.3186 0.1028 0.4637 -0.516 -0.3177 -0.3292 -0.886 0.021 -0.3079 -0.5934 -0.7061 -1.0166 0.6253 -0.3265 -1.0115
Actin 0.0376 0.01 0.0126 0.0215 0.0612 0.0102 0.0227 0.0018 0.014 0.0004 0.0079 0.009 0.0787 0.0452 0.0653 0.2179 0.0269 0.0235
Bud 0.0048 0.0028 0.0013 0.0026 0.0026 0.0024 0.0012 0.0028 0.0015 0.0001 0.002 0.001 0.0024 0.0012 0.001 0.0052 0.0008 0.0004
Bud Neck 0.005 0.0003 0.0021 0.0193 0.0008 0.0058 0.0042 0.0024 0.0022 0.0001 0.0007 0.0074 0.0029 0.0179 0.0014 0.0538 0.0037 0.0011
Bud Periphery 0.0106 0.0037 0.0094 0.0098 0.0096 0.0107 0.0026 0.0078 0.006 0.0005 0.0085 0.0035 0.0041 0.0019 0.0034 0.0078 0.0012 0.0008
Bud Site 0.015 0.008 0.0034 0.0128 0.002 0.0012 0.005 0.0264 0.0073 0.0001 0.0008 0.0007 0.0105 0.016 0.0071 0.0704 0.0023 0.001
Cell Periphery 0.0011 0.0007 0.0006 0.0027 0.0005 0.0009 0.0012 0.0024 0.002 0.0001 0.0004 0.0006 0.0014 0.0029 0.0021 0.0021 0.0007 0.0007
Cytoplasm 0.0522 0.0636 0.0528 0.0817 0.0837 0.0866 0.0657 0.0866 0.0919 0.0299 0.0422 0.0826 0.0788 0.1888 0.123 0.0388 0.1248 0.1863
Cytoplasmic Foci 0.0788 0.0552 0.0805 0.098 0.0854 0.0888 0.0986 0.0831 0.0857 0.0081 0.0493 0.0612 0.1146 0.0722 0.1125 0.0992 0.1631 0.0646
Eisosomes 0.0011 0.0007 0.0013 0.0005 0.0009 0.0005 0.0011 0.0012 0.0009 0.0001 0.0005 0.0004 0.0019 0.0011 0.0018 0.0032 0.0007 0.0009
Endoplasmic Reticulum 0.0076 0.0019 0.0024 0.0018 0.0023 0.0027 0.006 0.0032 0.006 0.0003 0.0019 0.0023 0.0208 0.0049 0.0046 0.0024 0.0091 0.0057
Endosome 0.0569 0.0465 0.0483 0.0417 0.0407 0.0403 0.0596 0.0492 0.0458 0.005 0.0498 0.0319 0.0778 0.0568 0.0372 0.0273 0.1447 0.0467
Golgi 0.0185 0.0201 0.0146 0.0174 0.0376 0.0162 0.021 0.0132 0.0116 0.0043 0.0154 0.0096 0.0241 0.0332 0.0124 0.043 0.068 0.0313
Lipid Particles 0.0296 0.0059 0.017 0.0122 0.0178 0.0171 0.0294 0.0056 0.0153 0.0013 0.0628 0.0119 0.0623 0.005 0.0194 0.0231 0.0534 0.0287
Mitochondria 0.0973 0.1526 0.0982 0.1545 0.1315 0.1518 0.0502 0.1056 0.0941 0.2674 0.2771 0.0707 0.0241 0.0794 0.0925 0.0435 0.1306 0.0679
None 0.5163 0.5713 0.5841 0.4627 0.4753 0.5052 0.5622 0.5715 0.5735 0.6761 0.4155 0.6659 0.4458 0.447 0.4857 0.2933 0.1961 0.4958
Nuclear Periphery 0.0068 0.0007 0.0051 0.0011 0.0046 0.0023 0.0086 0.0008 0.0035 0.0001 0.0055 0.0069 0.008 0.0012 0.0011 0.0011 0.0057 0.002
Nucleolus 0.0049 0.0011 0.0009 0.001 0.0008 0.0006 0.0015 0.0017 0.0015 0.0003 0.0012 0.0005 0.002 0.0007 0.0008 0.0013 0.0009 0.0011
Nucleus 0.0035 0.0017 0.0014 0.0012 0.0024 0.0011 0.0037 0.0024 0.0022 0.0004 0.0015 0.0011 0.0037 0.0012 0.0011 0.001 0.0015 0.0017
Peroxisomes 0.0382 0.0463 0.0521 0.0454 0.0292 0.0471 0.0365 0.0176 0.0201 0.0041 0.0355 0.0235 0.0188 0.0134 0.0215 0.0564 0.0136 0.0323
Punctate Nuclear 0.0052 0.0009 0.002 0.0021 0.007 0.002 0.0062 0.0036 0.0042 0.0002 0.0149 0.0037 0.0101 0.0024 0.0023 0.0046 0.0456 0.002
Vacuole 0.0065 0.0048 0.0073 0.0065 0.0023 0.0043 0.0105 0.0093 0.0087 0.0007 0.0033 0.004 0.0051 0.0061 0.0029 0.0035 0.0038 0.0043
Vacuole Periphery 0.0024 0.0012 0.0022 0.0033 0.0015 0.0022 0.0025 0.0019 0.0023 0.0004 0.0034 0.0016 0.002 0.0013 0.0011 0.001 0.0027 0.0013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 32.8208 27.9427 20.7347 33.6522 30.3954 31.2406 29.43 32.7347 37.8242 37.1141
Translational Efficiency 1.0212 1.0755 1.3678 0.9523 1.0043 1.2961 1.3349 0.9391 1.0441 1.0334

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2173 95 1899 1536 1873 1853 247 1182 4046 1948 2146 2718

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 669.09 767.76 786.94 797.59 665.22 698.49 907.21 811.27 667.30 701.87 800.78 803.54
Standard Deviation 72.96 104.43 98.03 98.89 73.68 78.13 96.03 126.81 73.32 81.00 105.06 112.10
Intensity Change Log 2 — 0.198455 0.234053 0.253447 — 0.070408 0.447605 0.286351 — 0.136037 0.344468 0.269945

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000545 0.001629 0.004137 0.003068 0.000642 0.001005 0.003253 0.002695 0.000590 0.001035 0.004035 0.002906
Bud Neck 0.051350 0.048665 0.036719 0.018980 0.044523 0.064793 0.014390 0.017490 0.048190 0.064007 0.034149 0.018332
Bud Site 0.018924 0.022273 0.048167 0.053477 0.013355 0.028972 0.032848 0.049275 0.016346 0.028646 0.046404 0.051650
Cell Periphery 0.000207 0.000170 0.000199 0.000218 0.000311 0.000211 0.000251 0.000182 0.000255 0.000209 0.000205 0.000202
Cytoplasm 0.230042* 0.216535* 0.233745* 0.371766* 0.229954* 0.251646* 0.158106* 0.421075* 0.230002* 0.249933* 0.225039* 0.393210*
Cytoplasmic Foci 0.325448* 0.281156* 0.055502 0.066684 0.285373* 0.303646* 0.101932 0.065090 0.306897* 0.302549* 0.060846 0.065991
Eisosomes 0.000340 0.000201 0.000086 0.000105 0.000287 0.000270 0.000171 0.000064 0.000315 0.000266 0.000096 0.000087
Endoplasmic Reticulum 0.001330 0.001028 0.005293 0.003443 0.001537 0.000618 0.006368 0.001350 0.001426 0.000638 0.005416 0.002533
Endosome 0.034155 0.069820 0.019059 0.015296 0.040880 0.042438 0.056062 0.023389 0.037268 0.043773 0.023318 0.018815
Golgi 0.024089 0.013722 0.012765 0.007071 0.031954 0.022252 0.016318 0.013683 0.027730 0.021836 0.013174 0.009946
Lipid Particles 0.020155 0.008279 0.003472 0.003162 0.025154 0.009928 0.007133 0.002114 0.022469 0.009847 0.003894 0.002707
Mitochondria 0.120335* 0.039483 0.051973 0.013959 0.127933* 0.067135 0.034684 0.012988 0.123853* 0.065787 0.049983 0.013537
Mitotic Spindle 0.007121 0.016773 0.069143 0.046901 0.007096 0.015361 0.058632 0.072249 0.007109 0.015430 0.067934 0.057925
None 0.001848 0.001558 0.002678 0.005512 0.002080 0.002039 0.002047 0.006219 0.001955 0.002015 0.002606 0.005819
Nuclear Periphery 0.000383 0.000400 0.002311 0.001794 0.000491 0.000274 0.001843 0.001387 0.000433 0.000280 0.002257 0.001617
Nuclear Periphery Foci 0.000190 0.001074 0.001405 0.001505 0.000332 0.000323 0.003862 0.000963 0.000256 0.000360 0.001688 0.001269
Nucleolus 0.001333 0.002924 0.002165 0.000859 0.001808 0.001386 0.002766 0.000963 0.001553 0.001461 0.002235 0.000904
Nucleus 0.072917 0.119316 0.336741* 0.188791* 0.090460 0.067245 0.322479* 0.148289* 0.081038 0.069784 0.335100* 0.171177*
Peroxisomes 0.039221 0.073576 0.040604 0.030583 0.046077 0.057570 0.064349 0.019514 0.042395 0.058350 0.043337 0.025770
Vacuole 0.045710 0.078318 0.070234 0.163313 0.044177 0.059432 0.109700 0.135469 0.045001 0.060353 0.074777 0.151204
Vacuole Periphery 0.004357 0.003097 0.003601 0.003512 0.005575 0.003457 0.002804 0.005552 0.004921 0.003440 0.003509 0.004399

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.83 -6.46 -5.55 -0.15 3.44 -2.15 -3.60 -1.12 -0.63 1.12 -3.20 -6.79 -3.19 -1.80 3.41
Bud Neck 0.51 4.84 13.75 3.30 9.06 -5.30 9.41 10.51 15.03 -0.55 -4.67 5.85 17.26 16.30 9.14
Bud Site -0.67 -8.96 -4.82 -2.38 2.91 -7.73 -2.32 -5.90 -0.63 -1.31 -6.49 -10.61 -7.52 -1.45 3.12
Cell Periphery 1.14 0.80 2.31 0.57 1.89 3.12 1.91 5.92 4.66 4.87 2.20 3.09 6.00 3.88 3.85
Cytoplasm 0.64 -0.13 -13.26 -5.18 -12.74 -2.59 5.34 -13.47 -11.26 -14.50 -2.83 1.09 -18.87 -13.23 -17.67
Cytoplasmic Foci 2.17 49.61 49.48 10.80 -0.08 -2.32 21.70 39.68 40.82 3.98 0.75 59.38 63.16 44.72 1.75
Eisosomes 2.39 5.15 5.01 4.53 -1.10 0.54 4.81 11.44 6.90 7.83 1.28 7.80 8.36 6.62 3.23
Endoplasmic Reticulum 0.58 -11.39 -6.13 -6.34 5.58 3.11 -7.37 -1.60 -10.80 7.17 3.82 -13.51 -6.01 -12.63 9.64
Endosome -2.74 9.52 14.36 4.77 4.61 -0.51 0.27 11.59 12.01 3.67 -2.63 10.30 18.23 14.91 5.13
Golgi 3.26 8.44 14.39 2.84 5.34 4.02 6.91 10.37 7.84 1.33 3.43 11.60 16.85 11.04 4.49
Lipid Particles 4.81 13.08 13.86 3.38 2.03 9.23 10.72 15.61 10.63 6.70 10.61 18.63 20.81 10.34 5.23
Mitochondria 6.37 13.72 22.99 2.39 10.53 9.53 11.75 21.99 15.42 1.99 11.80 18.35 31.82 16.09 10.96
Mitotic Spindle -1.36 -14.22 -8.23 -2.74 5.25 -4.15 -3.75 -9.32 -7.06 -1.06 -4.64 -14.78 -12.40 -8.05 4.03
None 0.12 -2.02 -1.99 -1.91 -1.28 0.03 -0.50 -2.86 -2.76 -2.44 -0.25 -2.37 -3.25 -2.89 -2.40
Nuclear Periphery -0.78 -14.16 -17.17 -14.72 -0.25 4.15 -8.86 -13.44 -15.46 0.99 3.73 -15.39 -21.75 -23.26 0.72
Nuclear Periphery Foci -2.30 -5.11 -8.62 -0.65 -0.48 0.03 -4.73 -7.06 -5.01 3.96 -1.21 -6.34 -10.58 -6.98 1.78
Nucleolus -2.42 -3.64 1.18 2.69 4.25 1.56 -1.76 2.07 0.82 2.65 0.34 -3.38 2.36 1.88 4.89
Nucleus -2.32 -35.25 -23.47 -4.76 14.51 5.02 -13.97 -15.02 -18.62 8.20 2.96 -37.39 -27.25 -27.19 17.64
Peroxisomes -2.49 1.27 7.69 3.92 5.53 -3.16 0.44 12.64 14.64 4.29 -5.15 2.26 13.83 14.37 8.08
Vacuole -2.90 -10.50 -30.17 -10.65 -22.55 -4.87 -9.07 -23.88 -21.62 -5.52 -5.81 -13.62 -38.36 -32.70 -25.23
Vacuole Periphery 2.12 3.32 5.53 1.63 2.24 4.11 5.50 3.50 1.01 -1.01 4.54 5.25 6.10 2.83 1.33
ORF
Human Ortholog
Description Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
Localization
Cell Percentages mitochondrion (44%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

YKR070W

YKR070W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ykr070w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available