Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – – 0 – 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 – – 0 – 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 – – 0 – 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 – – 0 – 0 – 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 – – 0 – 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0 0 – – 0 – 0.12 – 0.05 0.07 0 0.05 0 0 0 – – – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 – – 0 – 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0.18 0 – – 0.1 – 0.05 – 0 0 0 0 0 0 0.09 – – – 0.06 0.05 0 0.07 0.07 0.08
Golgi 0 0 – – 0.48 – 0.07 – 0 0 0 0 0.08 0.09 0.16 – – – 0 0.05 0.11 0.05 0 0.06
Mitochondria 0.66 0.9 – – 0.52 – 0.68 – 0.9 0.82 0.94 0.87 0.85 0.91 0.79 – – – 0.78 0.75 0.73 0.75 0.66 0.63
Nucleus 0 0 – – 0.05 – 0.05 – 0 0.05 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0.05 0.06 – – 0 – 0.13 – 0.06 0.05 0.06 0.06 0.07 0 0 – – – 0 0 0 0 0 0
Nucleolus 0.06 0 – – 0 – 0.07 – 0.06 0.05 0 0.06 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0.08 0 – – 0 – 0 – 0 0 0 0 0.07 0 0.07 – – – 0 0 0.1 0.06 0.09 0.07
SpindlePole 0 0 – – 0.1 – 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0.25 0.15 – – 0.14 – 0.08 – 0 0.05 0 0 0.12 0.1 0.11 – – – 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – – 0 – 0 – 0 0 0 0 1 2 1 – – – 6 1 0 2 4 4
Bud 0 1 – – 0 – 0 – 2 0 3 3 1 3 1 – – – 4 0 0 0 2 4
Bud Neck 0 0 – – 0 – 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 1 2
Bud Site 1 0 – – 0 – 1 – 1 0 1 0 0 0 0 – – – – – – – – –
Cell Periphery 16 1 – – 0 – 0 – 1 0 0 2 8 7 9 – – – 0 0 0 0 1 0
Cytoplasm 3 0 – – 0 – 12 – 5 10 1 8 8 6 8 – – – 0 0 0 0 1 1
Endoplasmic Reticulum 0 2 – – 0 – 2 – 0 3 0 3 2 0 5 – – – 1 0 0 0 1 0
Endosome 87 3 – – 2 – 5 – 0 0 0 2 7 7 29 – – – 32 4 0 11 24 25
Golgi 7 0 – – 10 – 7 – 0 2 0 3 15 23 52 – – – 17 3 1 8 15 17
Mitochondria 316 64 – – 11 – 66 – 84 113 84 153 153 242 260 – – – 443 60 13 132 235 195
Nucleus 6 0 – – 1 – 5 – 1 7 0 3 1 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 23 4 – – 0 – 13 – 6 7 5 10 12 7 8 – – – 13 2 0 2 9 9
Nucleolus 31 2 – – 0 – 7 – 6 7 4 10 7 4 5 – – – 0 0 0 0 7 10
Peroxisomes 40 0 – – 0 – 2 – 0 1 1 1 12 9 24 – – – 17 0 1 10 32 20
SpindlePole 17 0 – – 2 – 0 – 0 4 1 3 4 8 4 – – – 8 0 0 2 4 3
Vac/Vac Membrane 118 11 – – 3 – 8 – 4 7 3 6 21 27 37 – – – 14 4 0 3 9 11
Unique Cell Count 477 71 21 97 93 137 89 175 180 266 331 567 80 18 178 358 312
Labelled Cell Count 665 88 29 128 110 161 103 207 252 345 443 567 80 18 178 358 312


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.3 6.2 – – 8.2 – 6.7 – 6.1 6.7 5.6 6.2 7.6 6.7 7.5 – – – 6.8 7.2 7.5
Std Deviation (1e-4) 1.6 1.0 – – 1.7 – 1.7 – 1.4 1.9 1.1 1.4 1.6 1.6 1.5 – – – 1.8 1.8 1.6
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.5857 4.3916 3.8839 3.7503 3.37 4.1123 6.689 6.866 6.9253 5.6908 5.8544 6.998 7.3885 8.2777 7.8937 8.0821 6.9662 7.3821
Actin 0.0219 0.0031 0.0048 0.0527 0.0062 0.0185 0.021 0.0083 0.0057 0.0009 0.0025 0.0045 0.015 0.0015 0.0059 0.0001 0.0055 0.0166
Bud 0.0003 0.0007 0.0011 0.0009 0.0002 0.0001 0.0003 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0004 0.0028 0 0.0002 0.0001
Bud Neck 0.0003 0.0003 0.0002 0.0006 0.0006 0.0003 0.0002 0.0001 0.0002 0 0 0 0.0001 0.0004 0.0004 0 0.0011 0.0001
Bud Periphery 0.0005 0.0009 0.0021 0.0009 0.0002 0.0003 0.0007 0.0002 0.0004 0.0002 0.0002 0.0002 0.0004 0.0004 0.0029 0.0001 0.0004 0.0002
Bud Site 0.001 0.0025 0.0023 0.0172 0.0006 0.0002 0.0003 0.0002 0.0019 0.0001 0.0001 0.0001 0.0006 0.0095 0.0097 0 0.0023 0.0001
Cell Periphery 0.0002 0.0002 0.0003 0.0002 0.0001 0.0002 0.0003 0.0001 0.0004 0.0001 0.0001 0.0001 0.0002 0.0002 0.0003 0 0.0002 0.0001
Cytoplasm 0.0017 0 0.0027 0.0003 0.0002 0.0003 0.0018 0.0001 0.0009 0 0.0006 0 0.0002 0 0.0002 0 0.0001 0.0001
Cytoplasmic Foci 0.021 0.0036 0.0147 0.0063 0.0228 0.0037 0.0028 0.0022 0.0062 0.0003 0.0051 0.0011 0.0054 0.0037 0.0008 0.0001 0.0048 0.0013
Eisosomes 0.0003 0.0002 0.0002 0.0004 0.0002 0.0002 0.0004 0.0002 0.0004 0.0001 0.0001 0.0001 0.0004 0.0002 0.0005 0 0.0002 0.0004
Endoplasmic Reticulum 0.0018 0.0001 0.0086 0.0002 0.0001 0.0013 0.0014 0.0001 0.0009 0.0001 0.001 0.0002 0.0002 0.0001 0.0002 0 0.0001 0.0004
Endosome 0.032 0.0105 0.0209 0.0522 0.0215 0.0086 0.014 0.0119 0.0168 0.0025 0.0332 0.0068 0.0114 0.0088 0.0011 0.001 0.0326 0.0044
Golgi 0.037 0.0351 0.0075 0.0264 0.0789 0.0089 0.0157 0.0511 0.0196 0.0089 0.0104 0.0265 0.0187 0.041 0.002 0.0029 0.0503 0.0103
Lipid Particles 0.0236 0.025 0.0039 0.0047 0.0037 0.012 0.0145 0.0066 0.0126 0.0007 0.0156 0.003 0.0126 0.0029 0.0015 0.0002 0.0265 0.0103
Mitochondria 0.7846 0.8917 0.9134 0.7924 0.8133 0.9148 0.8747 0.9101 0.9051 0.984 0.9237 0.9518 0.9191 0.9103 0.9657 0.9948 0.8437 0.9421
None 0.004 0.0001 0.0006 0.0002 0.0001 0.0003 0.0017 0 0.0023 0 0.0004 0.0001 0.0008 0 0.0003 0 0.0001 0.0006
Nuclear Periphery 0.0041 0.0001 0.004 0.0001 0 0.0057 0.0134 0.0001 0.0031 0 0.0019 0.0005 0.0008 0 0.0001 0 0.0001 0.0021
Nucleolus 0.0008 0.0003 0.0001 0.0001 0 0.0024 0.0002 0 0.0003 0 0 0 0.0009 0.0004 0.0002 0 0.0009 0.0001
Nucleus 0.001 0.0001 0.0006 0.0001 0 0.0018 0.0027 0 0.0055 0 0.0001 0.0001 0.0002 0.0001 0.0001 0 0.0002 0.0001
Peroxisomes 0.031 0.0167 0.0059 0.0421 0.049 0.0141 0.0081 0.0031 0.0055 0.0006 0.0025 0.0017 0.005 0.0169 0.0035 0.0002 0.0183 0.0049
Punctate Nuclear 0.0057 0 0.0003 0.0003 0.0003 0.0011 0.0017 0 0.0013 0 0.0011 0.0003 0.0021 0 0 0 0.0001 0.0021
Vacuole 0.0105 0.003 0.0031 0.0006 0.0005 0.0011 0.0059 0.0009 0.0032 0.0004 0.0004 0.0003 0.0021 0.0012 0.0006 0.0001 0.0036 0.0004
Vacuole Periphery 0.0164 0.0059 0.0029 0.0014 0.0014 0.004 0.0182 0.0043 0.0077 0.001 0.0009 0.0024 0.0035 0.0018 0.0013 0.0004 0.0086 0.0035

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 49.7283 38.5827 43.5675 37.1742 34.0622 50.6385 41.1311 56.6663 40.933 49.5838
Translational Efficiency 1.0526 1.2243 0.9776 1.2374 1.6398 1.5079 1.4471 1.156 1.5033 1.3973

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1947 419 255 1491 2100 144 244 1279 4047 563 499 2770

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 679.08 883.38 1076.26 1070.12 776.83 972.48 1008.26 1068.34 729.80 906.17 1043.01 1069.30
Standard Deviation 101.73 132.11 134.00 161.05 102.66 130.90 154.88 167.30 113.28 137.42 148.53 163.97
Intensity Change Log 2 — 0.379453 0.664373 0.656119 — 0.324070 0.376197 0.459700 — 0.350167 0.517794 0.554651

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000402 0.000706 0.001718 0.000949 0.000202 0.000790 0.001032 0.001015 0.000298 0.000727 0.001382 0.000979
Bud Neck 0.003578 0.009514 0.014884 0.026372 0.004651 0.002388 0.010833 0.036433 0.004135 0.007691 0.012903 0.031017
Bud Site 0.001131 0.001302 0.001798 0.002559 0.000336 0.000632 0.001028 0.003902 0.000718 0.001131 0.001421 0.003179
Cell Periphery 0.000236 0.000076 0.000045 0.000067 0.000054 0.000053 0.000031 0.000087 0.000142 0.000070 0.000038 0.000076
Cytoplasm 0.013152 0.003119 0.000224 0.001201 0.001404 0.000279 0.000889 0.002210 0.007056 0.002393 0.000549 0.001667
Cytoplasmic Foci 0.001690 0.000681 0.001882 0.000846 0.000218 0.001422 0.000030 0.000483 0.000927 0.000870 0.000976 0.000678
Eisosomes 0.000025 0.000037 0.000058 0.000049 0.000021 0.000038 0.000046 0.000042 0.000023 0.000037 0.000052 0.000046
Endoplasmic Reticulum 0.003522 0.003139 0.003708 0.002102 0.001563 0.003566 0.001996 0.002749 0.002506 0.003248 0.002871 0.002400
Endosome 0.000560 0.000253 0.001112 0.001044 0.000241 0.002336 0.000067 0.000770 0.000394 0.000786 0.000601 0.000918
Golgi 0.000329 0.000168 0.005198 0.000657 0.000097 0.000442 0.000156 0.000984 0.000209 0.000238 0.002733 0.000808
Lipid Particles 0.000485 0.000576 0.000114 0.000109 0.000173 0.001034 0.000040 0.000126 0.000323 0.000693 0.000078 0.000117
Mitochondria 0.001564 0.001145 0.001572 0.002317 0.001155 0.003155 0.001029 0.002466 0.001352 0.001659 0.001307 0.002386
Mitotic Spindle 0.000788 0.000821 0.002622 0.002967 0.002433 0.008321 0.001016 0.011468 0.001642 0.002740 0.001837 0.006892
None 0.027460 0.002931 0.001454 0.001382 0.003856 0.001705 0.001974 0.003309 0.015211 0.002617 0.001708 0.002272
Nuclear Periphery 0.000319 0.000152 0.000193 0.000572 0.000213 0.000115 0.000074 0.000853 0.000264 0.000142 0.000135 0.000702
Nuclear Periphery Foci 0.001397 0.000373 0.000189 0.000424 0.000455 0.001244 0.000155 0.000382 0.000908 0.000595 0.000172 0.000405
Nucleolus 0.066632 0.115149 0.093103 0.061379 0.075314 0.084029 0.105483 0.043745 0.071137 0.107189 0.099157 0.053237
Nucleus 0.875462* 0.857943* 0.862004* 0.891925* 0.906904* 0.879995* 0.871819* 0.885875* 0.891778* 0.863583* 0.866803* 0.889132*
Peroxisomes 0.000475 0.001282 0.002208 0.000752 0.000298 0.004714 0.001808 0.000596 0.000383 0.002160 0.002012 0.000680
Vacuole 0.000586 0.000360 0.002976 0.001480 0.000216 0.001854 0.000336 0.001812 0.000394 0.000742 0.001685 0.001633
Vacuole Periphery 0.000206 0.000273 0.002936 0.000849 0.000196 0.001889 0.000159 0.000693 0.000200 0.000687 0.001578 0.000777

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.72 -12.82 -10.07 -4.45 6.54 -2.34 -12.97 -12.99 -1.59 -1.67 -5.70 -16.85 -16.58 -4.55 4.28
Bud Neck -5.57 -10.77 -18.72 -11.88 -6.42 4.58 -6.31 -21.20 -22.29 -14.48 -4.43 -12.10 -28.03 -20.98 -14.84
Bud Site -0.80 -4.11 -2.99 -2.42 -0.83 -1.38 -6.27 -4.61 -4.18 -3.57 -2.10 -6.59 -5.59 -4.40 -3.43
Cell Periphery 4.15 5.73 4.99 0.55 -1.47 0.09 2.04 -3.01 -1.73 -5.88 3.10 6.03 3.70 -0.28 -4.64
Cytoplasm 4.49 9.37 8.13 1.10 -2.45 3.49 0.96 -1.52 -3.72 -1.99 3.14 8.79 6.74 0.51 -2.90
Cytoplasmic Foci 1.56 0.83 2.55 0.21 0.44 -1.41 3.62 -0.51 1.24 -1.61 0.27 0.93 2.06 0.79 0.13
Eisosomes -5.25 -9.43 -12.65 -5.07 3.52 -3.64 -9.90 -14.82 -1.32 1.08 -7.23 -13.18 -19.38 -4.62 3.44
Endoplasmic Reticulum 1.02 -0.87 5.25 3.36 3.93 -1.86 -2.50 -4.59 0.34 -3.32 -2.17 -1.86 -0.97 1.51 0.95
Endosome 2.23 -0.20 -0.48 -1.65 -0.18 -1.41 2.71 -1.72 1.16 -2.75 -0.87 0.14 -1.28 0.11 -1.08
Golgi 2.30 -1.17 -0.73 -1.41 1.08 -1.90 -3.35 -1.35 -0.57 -1.21 -0.30 -1.20 -1.58 -1.50 0.86
Lipid Particles -0.26 4.15 4.36 1.34 0.81 -1.33 4.35 1.09 1.42 -2.35 -1.11 5.31 4.39 1.82 -0.86
Mitochondria 1.22 -0.55 -2.50 -5.04 -3.00 -1.04 0.15 -3.63 0.20 -6.21 -0.54 -0.33 -4.34 -2.00 -6.30
Mitotic Spindle -0.18 -1.50 -4.04 -3.69 -1.54 -0.84 0.03 -5.04 -1.24 -4.19 -0.58 -0.90 -6.30 -3.87 -4.22
None 11.92 13.13 13.09 1.66 -0.59 3.06 3.12 0.51 -2.96 -3.13 11.34 13.23 12.42 -0.02 -2.86
Nuclear Periphery 3.32 1.95 -2.44 -3.57 -3.10 1.54 2.52 -6.02 -6.54 -6.88 3.15 3.03 -5.86 -6.98 -6.90
Nuclear Periphery Foci 3.06 4.31 3.08 -0.11 -1.29 -0.66 2.50 0.11 0.68 -3.10 1.05 4.96 2.75 0.48 -2.59
Nucleolus -8.14 -5.31 1.02 8.38 5.63 -1.01 -4.51 8.76 4.49 8.24 -7.31 -6.92 6.47 10.40 9.61
Nucleus 2.08 2.78 -1.33 -3.11 -3.51 1.76 5.13 6.82 0.86 -0.37 4.17 5.46 3.91 -1.61 -2.98
Peroxisomes -3.27 -7.55 -1.37 2.78 7.57 -1.33 -7.69 -3.27 1.23 5.69 -2.02 -10.76 -3.16 1.71 9.40
Vacuole 1.58 -1.66 -2.41 -3.06 0.92 -1.04 -1.31 -3.48 -0.48 -3.29 -0.83 -1.78 -4.28 -2.34 -0.66
Vacuole Periphery -2.20 -1.04 -2.11 -1.84 0.71 -1.00 0.30 -2.07 0.79 -2.41 -1.12 -1.03 -2.85 0.07 0.48
Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Mrpl20

Mrpl20


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrpl20-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available