Standard name | |
---|---|
Human Ortholog | |
Description | Multifunctional lipase/hydrolase/phospholipase; triacylglycerol lipase, steryl ester hydrolase, and Ca2+-independent phospholipase A2; catalyzes acyl-CoA dependent acylation of LPA to PA; required with Tgl3p for timely bud formation; phosphorylated and activated by Cdc28p; TGL4 has a paralog, TGL5, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 4 | 2 | 2 | 0 | 1 | 3 | 3 | 0 | – | 0 | 2 | 2 | 1 | 0 | 1 | 1 | 1 | 1 | 11 | 0 | 0 | 2 | 3 |
Bud | 2 | 4 | 9 | 3 | 7 | 5 | 12 | 10 | 6 | – | 8 | 4 | 3 | 0 | 1 | 0 | 0 | 0 | 4 | 4 | 1 | 0 | 3 | 1 |
Bud Neck | 1 | 1 | 3 | 1 | 3 | 1 | 0 | 1 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 3 | 2 | 3 | 0 | 2 | 3 | 2 | 1 | 1 | – | 3 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 19 | 4 | 10 | 7 | 16 | 11 | 23 | 20 | 8 | – | 3 | 5 | 12 | 10 | 13 | 26 | 36 | 30 | 1 | 0 | 2 | 0 | 0 | 0 |
Endoplasmic Reticulum | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 2 | 0 | – | 0 | 0 | 0 | 1 | 2 | 2 | 7 | 3 | 5 | 0 | 2 | 0 | 1 | 2 |
Endosome | 91 | 157 | 111 | 74 | 74 | 64 | 53 | 85 | 27 | – | 38 | 46 | 105 | 220 | 148 | 130 | 183 | 106 | 158 | 294 | 177 | 33 | 92 | 103 |
Golgi | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 3 | 9 | 0 | 9 | 22 | 5 | 0 | 8 | 6 |
Mitochondria | 114 | 233 | 45 | 16 | 26 | 49 | 106 | 108 | 185 | – | 189 | 148 | 58 | 32 | 35 | 28 | 30 | 18 | 11 | 35 | 5 | 3 | 15 | 6 |
Nucleus | 2 | 2 | 0 | 0 | 1 | 5 | 4 | 7 | 5 | – | 11 | 5 | 1 | 3 | 3 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 7 | 47 | 13 | 2 | 3 | 5 | 8 | 15 | 23 | – | 31 | 31 | 3 | 2 | 6 | 4 | 6 | 3 | 2 | 9 | 1 | 0 | 0 | 0 |
Nucleolus | 97 | 91 | 27 | 35 | 41 | 53 | 64 | 76 | 73 | – | 87 | 71 | 69 | 101 | 112 | 90 | 97 | 67 | 3 | 10 | 1 | 0 | 3 | 0 |
Peroxisomes | 76 | 220 | 57 | 68 | 50 | 47 | 22 | 18 | 18 | – | 6 | 7 | 218 | 266 | 194 | 88 | 113 | 72 | 79 | 176 | 81 | 46 | 158 | 113 |
SpindlePole | 117 | 133 | 141 | 111 | 205 | 153 | 166 | 158 | 71 | – | 57 | 68 | 119 | 214 | 127 | 133 | 224 | 122 | 77 | 93 | 46 | 17 | 35 | 26 |
Vac/Vac Membrane | 7 | 8 | 2 | 4 | 3 | 3 | 5 | 1 | 3 | – | 3 | 8 | 0 | 6 | 2 | 12 | 17 | 15 | 3 | 2 | 1 | 0 | 0 | 0 |
Unique Cell Count | 334 | 579 | 281 | 205 | 318 | 261 | 327 | 341 | 292 | 290 | 276 | 360 | 458 | 371 | 309 | 427 | 267 | 371 | 676 | 334 | 105 | 332 | 273 | |
Labelled Cell Count | 538 | 908 | 425 | 324 | 433 | 400 | 469 | 505 | 421 | 436 | 396 | 591 | 858 | 643 | 518 | 726 | 438 | 371 | 676 | 334 | 105 | 332 | 273 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.0 | 7.7 | 6.6 | 6.2 | 6.8 | 5.2 | 5.7 | 5.8 | 4.8 | – | 4.7 | 5.2 | 7.2 | 7.5 | 7.5 | 9.4 | 9.8 | 9.9 | 6.5 | 6.8 | 7.6 |
Std Deviation (1e-4) | 1.3 | 1.4 | 1.2 | 1.2 | 1.5 | 1.5 | 1.8 | 1.5 | 1.0 | – | 1.2 | 1.1 | 1.8 | 1.4 | 1.5 | 1.8 | 2.1 | 1.9 | 1.6 | 1.9 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.1 | 0.04 | -0.34 | -0.22 | -0.19 | -0.47 | – | -0.51 | -0.35 | 0.11 | 0.18 | 0.17 | 0.51 | 0.56 | 0.58 | -0.02 | 0.04 | 0.19 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | -1.2 | -0.8 | -0.9 | 0.3 | -0.2 | 0 | – | 0 | -1.4 | -2.2 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.1 | 0.9 | 0.4 | 1.9 | 1.3 | -0.6 | – | -2.0 | -1.3 | -0.2 | -1.1 | 0 | 2.5 | 2.6 | 3.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -0.8 | -4.3 | -3.7 | -6.5 | -3.9 | 0 | – | 0 | -6.0 | -2.7 | 2.3 | 0.1 | 0.6 | 0.9 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -2.7 | -3.0 | 0.8 | 4.7 | 4.5 | 0 | – | 0 | 9.3 | 0 | -3.9 | -2.5 | -2.6 | -3.8 | -3.4 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -2.3 | -2.8 | -1.8 | -1.5 | -0.1 | 0 | – | 0 | 2.9 | -3.1 | -3.9 | -2.3 | -2.4 | -2.6 | -2.4 |
Nucleolus | 2.4 | 1.3 | 3.5 | 3.4 | 4.2 | 0 | – | 0 | 5.0 | 3.4 | 4.3 | 6.4 | 5.9 | 4.5 | 4.8 |
Peroxisomes | 3.2 | -1.5 | -0.7 | -5.0 | -5.7 | 0 | – | 0 | -6.6 | 10.2 | 10.1 | 8.3 | 2.3 | 1.9 | 1.8 |
SpindlePole | 0.9 | 3.5 | 2.0 | 0.1 | -1.0 | 0 | – | 0 | -6.2 | -4.4 | -0.9 | -4.1 | -1.7 | 0.6 | -1.1 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2.5 | 2.6 | 3.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.4668 | 4.8158 | 3.9399 | 3.7713 | 4.0164 | 4.2637 | 1.9579 | 3.1331 | 2.5915 | 2.0174 | 2.0 | 2.4297 | 2.652 | 3.6059 | 3.6168 | 3.5742 | 2.8594 | 3.3856 |
Actin | 0.0391 | 0.0035 | 0.0177 | 0.0001 | 0.0368 | 0.0047 | 0.0483 | 0.0001 | 0.0081 | 0 | 0.0011 | 0.0004 | 0.0009 | 0.001 | 0.0009 | 0.0002 | 0.0013 | 0.0004 |
Bud | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0006 | 0.0003 | 0.0004 | 0 | 0.0018 | 0.0002 |
Bud Neck | 0.0023 | 0.0006 | 0.0018 | 0.0001 | 0.0001 | 0.0003 | 0.0009 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0007 | 0.0004 | 0.0011 | 0.0002 | 0.0011 | 0.0006 |
Bud Periphery | 0.0002 | 0.0003 | 0.0002 | 0 | 0.0001 | 0.0005 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0005 | 0.0002 | 0.0005 | 0 | 0.0023 | 0.0002 |
Bud Site | 0.0017 | 0.0083 | 0.0083 | 0.0001 | 0.0002 | 0.0002 | 0.0068 | 0.0002 | 0.0006 | 0 | 0.0002 | 0.0001 | 0.0016 | 0.0042 | 0.0084 | 0.0001 | 0.0092 | 0.0004 |
Cell Periphery | 0.0001 | 0.0006 | 0.0003 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0005 | 0.0001 |
Cytoplasm | 0.0017 | 0.0001 | 0.0004 | 0.0002 | 0.001 | 0.0002 | 0.0023 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0014 | 0.0011 | 0.0004 | 0.0005 | 0.0037 | 0.0014 |
Cytoplasmic Foci | 0.1913 | 0.1755 | 0.1737 | 0.1235 | 0.2295 | 0.1462 | 0.1832 | 0.1856 | 0.1771 | 0.1759 | 0.1762 | 0.13 | 0.1799 | 0.1843 | 0.1831 | 0.2038 | 0.1316 | 0.1497 |
Eisosomes | 0.0003 | 0.0005 | 0.0007 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0007 | 0.0001 | 0.0003 | 0 | 0.0005 | 0.0005 | 0.0014 | 0.0002 | 0.0001 | 0 | 0.0002 | 0 | 0.0008 | 0.0003 | 0.0001 | 0.0002 | 0.0009 | 0.0002 |
Endosome | 0.0095 | 0.0047 | 0.0044 | 0.0483 | 0.0107 | 0.0067 | 0.0529 | 0.0215 | 0.017 | 0.0087 | 0.0218 | 0.0058 | 0.0325 | 0.0355 | 0.019 | 0.0158 | 0.0307 | 0.0104 |
Golgi | 0.0116 | 0.0085 | 0.0087 | 0.012 | 0.0124 | 0.0108 | 0.0201 | 0.0163 | 0.0209 | 0.012 | 0.0168 | 0.0109 | 0.0201 | 0.022 | 0.0158 | 0.0189 | 0.017 | 0.013 |
Lipid Particles | 0.6206 | 0.6568 | 0.6608 | 0.7026 | 0.5822 | 0.752 | 0.5767 | 0.6613 | 0.6879 | 0.7086 | 0.6042 | 0.7912 | 0.5834 | 0.5901 | 0.5902 | 0.612 | 0.585 | 0.6701 |
Mitochondria | 0.0016 | 0.004 | 0.0012 | 0.0014 | 0.0012 | 0.0031 | 0.0028 | 0.0009 | 0.0011 | 0.0003 | 0.0161 | 0.004 | 0.0102 | 0.003 | 0.008 | 0.0019 | 0.0248 | 0.0015 |
None | 0.0071 | 0.0002 | 0.004 | 0 | 0.0084 | 0.0001 | 0.0011 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0028 | 0.0005 | 0.0008 | 0.0002 | 0.0138 | 0.0014 |
Nuclear Periphery | 0.0009 | 0.0002 | 0.0007 | 0.0001 | 0.0035 | 0.0036 | 0.0052 | 0.0054 | 0.0001 | 0 | 0.0005 | 0.0004 | 0.0059 | 0.006 | 0.0017 | 0.0131 | 0.0042 | 0.0031 |
Nucleolus | 0.0035 | 0.0161 | 0.0048 | 0.0002 | 0.0003 | 0.0015 | 0.0041 | 0.013 | 0.0081 | 0.0002 | 0.0008 | 0.0026 | 0.0162 | 0.0246 | 0.014 | 0.0011 | 0.0113 | 0.01 |
Nucleus | 0.0006 | 0.0005 | 0.0016 | 0 | 0.0003 | 0.0065 | 0.0012 | 0.0005 | 0.0004 | 0 | 0.0002 | 0.0001 | 0.0078 | 0.0029 | 0.0022 | 0.0009 | 0.0135 | 0.0036 |
Peroxisomes | 0.0848 | 0.066 | 0.0836 | 0.1024 | 0.0976 | 0.0424 | 0.0663 | 0.0542 | 0.0512 | 0.0908 | 0.1123 | 0.0306 | 0.0765 | 0.0692 | 0.0644 | 0.0791 | 0.0576 | 0.0679 |
Punctate Nuclear | 0.022 | 0.0528 | 0.026 | 0.0077 | 0.0142 | 0.0183 | 0.0189 | 0.0364 | 0.0256 | 0.0031 | 0.0445 | 0.0233 | 0.0548 | 0.0478 | 0.0867 | 0.0511 | 0.0689 | 0.0646 |
Vacuole | 0.0003 | 0.0004 | 0.0003 | 0.0009 | 0.0001 | 0.0011 | 0.0049 | 0.0024 | 0.0007 | 0.0001 | 0.0019 | 0.0002 | 0.0019 | 0.0044 | 0.0012 | 0.0004 | 0.0175 | 0.0007 |
Vacuole Periphery | 0.0003 | 0.0002 | 0.0002 | 0.0003 | 0.0003 | 0.0009 | 0.002 | 0.0016 | 0.0003 | 0.0002 | 0.0027 | 0.0002 | 0.0011 | 0.0021 | 0.0007 | 0.0005 | 0.0033 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 17.5624 | 18.4066 | 23.0319 | 32.8261 | 24.0791 | 24.6141 | 24.9057 | 32.511 | 32.6554 | 28.0112 |
Translational Efficiency | 0.5727 | 0.5298 | 0.4467 | 0.5268 | 0.4365 | 0.4962 | 0.5067 | 0.4159 | 0.502 | 0.4407 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1978 | 1356 | 2160 | 1376 | 1874 | 1516 | 265 | 904 | 3852 | 2872 | 2425 | 2280 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 775.69 | 847.10 | 1097.35 | 1009.54 | 794.68 | 852.49 | 1026.98 | 1026.86 | 784.93 | 849.95 | 1089.66 | 1016.41 |
Standard Deviation | 103.13 | 138.34 | 145.81 | 171.12 | 107.62 | 125.04 | 128.90 | 204.88 | 105.76 | 131.51 | 145.72 | 185.44 |
Intensity Change Log 2 | — | 0.127052 | 0.500472 | 0.380146 | — | 0.101309 | 0.369962 | 0.369794 | — | 0.114082 | 0.435903 | 0.374916 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000847 | 0.002211 | 0.008563 | 0.057057 | 0.000463 | 0.001713 | 0.010341 | 0.032805 | 0.000660 | 0.001948 | 0.008758 | 0.047442 |
Bud Neck | 0.000417 | 0.001268 | 0.004675 | 0.003231 | 0.000612 | 0.001252 | 0.005577 | 0.004231 | 0.000512 | 0.001260 | 0.004774 | 0.003628 |
Bud Site | 0.000319 | 0.000471 | 0.004557 | 0.004693 | 0.000205 | 0.000626 | 0.003244 | 0.003067 | 0.000264 | 0.000553 | 0.004413 | 0.004048 |
Cell Periphery | 0.000042 | 0.000055 | 0.000080 | 0.000135 | 0.000029 | 0.000055 | 0.000096 | 0.000133 | 0.000036 | 0.000055 | 0.000082 | 0.000134 |
Cytoplasm | 0.000421 | 0.000556 | 0.002157 | 0.001978 | 0.000312 | 0.000489 | 0.001002 | 0.001876 | 0.000368 | 0.000520 | 0.002031 | 0.001937 |
Cytoplasmic Foci | 0.042223 | 0.097969 | 0.145888 | 0.236930* | 0.060182 | 0.088203 | 0.171129 | 0.207237* | 0.050960 | 0.092814 | 0.148646 | 0.225157* |
Eisosomes | 0.000144 | 0.000195 | 0.000049 | 0.000246 | 0.000108 | 0.000125 | 0.000120 | 0.000242 | 0.000126 | 0.000158 | 0.000057 | 0.000244 |
Endoplasmic Reticulum | 0.000385 | 0.000650 | 0.001323 | 0.001057 | 0.000347 | 0.000417 | 0.000871 | 0.000709 | 0.000366 | 0.000527 | 0.001274 | 0.000919 |
Endosome | 0.009990 | 0.028947 | 0.078148 | 0.103614 | 0.013092 | 0.042540 | 0.081406 | 0.089392 | 0.011499 | 0.036122 | 0.078504 | 0.097975 |
Golgi | 0.024629 | 0.050587 | 0.008360 | 0.131926* | 0.022895 | 0.036308 | 0.031974 | 0.143572* | 0.023785 | 0.043050 | 0.010940 | 0.136544* |
Lipid Particles | 0.863010* | 0.731339* | 0.386332* | 0.270944* | 0.831559* | 0.718455* | 0.427875* | 0.374775* | 0.847709* | 0.724538* | 0.390872* | 0.312112* |
Mitochondria | 0.002514 | 0.002266 | 0.001300 | 0.029514 | 0.001254 | 0.002581 | 0.002560 | 0.020324 | 0.001901 | 0.002432 | 0.001437 | 0.025870 |
Mitotic Spindle | 0.009606 | 0.017416 | 0.224964* | 0.039475 | 0.013949 | 0.013225 | 0.140128* | 0.035701 | 0.011719 | 0.015204 | 0.215693* | 0.037978 |
None | 0.000403 | 0.000077 | 0.000034 | 0.000107 | 0.000086 | 0.000068 | 0.000035 | 0.000412 | 0.000249 | 0.000072 | 0.000034 | 0.000228 |
Nuclear Periphery | 0.000532 | 0.000862 | 0.003406 | 0.001694 | 0.000490 | 0.000613 | 0.003498 | 0.003155 | 0.000511 | 0.000730 | 0.003416 | 0.002273 |
Nuclear Periphery Foci | 0.017435 | 0.021465 | 0.062543 | 0.003648 | 0.020742 | 0.027279 | 0.050368 | 0.008158 | 0.019044 | 0.024534 | 0.061213 | 0.005436 |
Nucleolus | 0.001239 | 0.001431 | 0.002497 | 0.000131 | 0.001182 | 0.001280 | 0.000478 | 0.000575 | 0.001211 | 0.001351 | 0.002276 | 0.000307 |
Nucleus | 0.000630 | 0.000354 | 0.001030 | 0.000197 | 0.000196 | 0.000455 | 0.000269 | 0.000186 | 0.000419 | 0.000407 | 0.000947 | 0.000193 |
Peroxisomes | 0.024759 | 0.039978 | 0.055777 | 0.098018 | 0.031611 | 0.062919 | 0.060335 | 0.053718 | 0.028092 | 0.052088 | 0.056275 | 0.080454 |
Vacuole | 0.000363 | 0.001605 | 0.007849 | 0.011548 | 0.000600 | 0.001206 | 0.008013 | 0.014039 | 0.000478 | 0.001395 | 0.007867 | 0.012536 |
Vacuole Periphery | 0.000095 | 0.000298 | 0.000469 | 0.003856 | 0.000086 | 0.000192 | 0.000682 | 0.005694 | 0.000091 | 0.000242 | 0.000493 | 0.004585 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.89 | -6.77 | -14.99 | -14.53 | -12.44 | -1.90 | -4.60 | -9.28 | -8.78 | -5.53 | -3.12 | -7.81 | -17.57 | -16.90 | -13.63 |
Bud Neck | -4.65 | -12.39 | -8.38 | -5.38 | 3.80 | -2.79 | -5.36 | -7.71 | -5.99 | 1.28 | -5.07 | -13.25 | -11.30 | -7.97 | 3.49 |
Bud Site | -1.18 | -9.39 | -6.92 | -6.70 | 0.81 | -2.42 | -5.46 | -8.33 | -6.58 | 0.15 | -2.57 | -10.20 | -9.42 | -8.58 | 1.67 |
Cell Periphery | -1.35 | -4.45 | -8.66 | -10.59 | -6.29 | -3.05 | -7.19 | -8.24 | -5.40 | -2.55 | -2.89 | -8.22 | -12.76 | -10.26 | -6.34 |
Cytoplasm | -1.35 | -4.18 | -11.80 | -14.59 | 1.41 | -3.90 | -4.60 | -3.93 | -3.54 | -2.09 | -2.69 | -4.45 | -8.64 | -8.07 | 1.10 |
Cytoplasmic Foci | -17.32 | -27.22 | -34.75 | -22.67 | -14.43 | -8.75 | -11.04 | -23.42 | -18.25 | -3.26 | -18.49 | -27.68 | -41.28 | -29.51 | -15.02 |
Eisosomes | -2.78 | 8.96 | -6.33 | -2.66 | -16.50 | -1.50 | -0.39 | -7.51 | -6.65 | -4.39 | -2.96 | 9.85 | -10.05 | -6.90 | -18.80 |
Endoplasmic Reticulum | -3.75 | -3.46 | -5.69 | -3.17 | 1.38 | -0.97 | -4.96 | -5.88 | -4.63 | 1.63 | -3.41 | -3.70 | -7.31 | -4.97 | 1.82 |
Endosome | -8.49 | -24.07* | -26.18* | -18.57 | -6.04 | -10.45 | -8.57 | -17.77 | -9.48 | -0.90 | -13.49 | -25.51 | -31.43 | -19.55 | -5.51 |
Golgi | -11.25 | 15.00 | -24.67 | -17.23 | -29.07* | -7.62 | -2.28 | -21.14 | -18.46 | -16.64 | -13.40 | 14.23 | -32.56 | -25.84 | -36.54* |
Lipid Particles | 15.56 | 56.91* | 66.67* | 43.98* | 10.01 | 12.39 | 19.30 | 38.21* | 26.64 | 2.18 | 19.79 | 60.75 | 73.92 | 50.63* | 7.46 |
Mitochondria | 0.42 | 2.14 | -9.41 | -9.60 | -9.96 | -1.94 | -1.09 | -7.11 | -6.60 | -6.22 | -1.14 | 1.09 | -11.80 | -11.58 | -12.12 |
Mitotic Spindle | -2.88 | -31.00* | -8.05 | -5.41 | 24.59* | 0.60 | -8.05 | -4.64 | -4.99 | 6.51 | -1.67 | -31.76 | -8.97 | -7.63 | 26.10* |
None | 1.16 | 1.29 | 1.06 | -2.16 | -6.07 | 0.67 | 1.73 | -1.09 | -1.19 | -1.30 | 1.24 | 1.46 | 0.13 | -1.34 | -1.62 |
Nuclear Periphery | -3.40 | -12.15 | -3.96 | -2.79 | 4.76 | -2.02 | -3.62 | -5.51 | -5.24 | 0.35 | -3.88 | -12.83 | -6.75 | -5.87 | 3.46 |
Nuclear Periphery Foci | -1.50 | -12.18 | 6.84 | 7.62 | 17.75 | -1.95 | -3.22 | 5.24 | 6.71 | 4.69 | -2.53 | -12.68 | 8.87 | 10.48 | 17.85 |
Nucleolus | -0.55 | -1.94 | 3.19 | 3.61 | 4.08 | -0.08 | 1.68 | 1.33 | 1.29 | -0.39 | -0.39 | -1.89 | 3.23 | 3.32 | 3.78 |
Nucleus | 1.06 | -1.94 | 1.65 | 1.23 | 3.84 | -1.16 | -0.65 | -0.02 | 1.16 | 0.64 | 0.07 | -2.92 | 1.52 | 1.56 | 4.03 |
Peroxisomes | -4.17 | -8.89 | -13.53 | -9.78 | -7.46 | -7.07 | -3.30 | -4.32 | 1.61 | 0.67 | -8.28 | -8.93 | -13.50 | -6.57 | -5.64 |
Vacuole | -7.57 | -10.55 | -8.17 | -7.21 | -1.32 | -4.20 | -4.31 | -8.68 | -8.28 | -2.25 | -8.51 | -11.18 | -11.70 | -10.77 | -2.47 |
Vacuole Periphery | -5.63 | -6.03 | -6.65 | -6.28 | -5.93 | -3.28 | -4.36 | -5.49 | -5.39 | -4.86 | -6.30 | -7.02 | -8.49 | -8.19 | -7.66 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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