Standard name
Human Ortholog
Description Myb-related transcription factor; involved in regulating basal and induced expression of genes of the purine and histidine biosynthesis pathways; also involved in regulation of meiotic recombination at specific genes
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0.06 0 0 – 0.06 0.07 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.07 0.06 0.09 0.07 0.1 – 0.15 0.05 0.05 0.09 0.11 0.06 0.08 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.14 0.14 0.07 0.17 0.11 0.39 0.5 0.26 0.55 – 0.57 0.49 0.06 0 0.07 0 0 0 0 0 0 0 0 0
Nucleus 0.92 0.87 0.92 0.9 0.86 0.7 0.7 0.74 0.62 – 0.59 0.75 0.92 0.86 0.79 0.83 0.84 0.83 0.85 0.77 0.84 0.64 0.54 0.47
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.1 0.05 0 0 0 0 0 0 0 – 0.05 0.06 0.05 0.06 0.05 0.07 0 0 0.08 0.06 0 0.07 0.11 0.14
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.07 0.09 0.06 0.1 0 – 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0 0.07 0.08 0.15 0.21 0.2
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 1 0 0 0 1
Bud 0 1 1 0 0 2 9 8 5 – 2 8 1 0 1 0 0 0 0 3 0 2 5 6
Bud Neck 0 0 1 0 0 0 10 7 4 – 2 3 0 0 0 0 0 0 0 0 0 1 3 3
Bud Site 0 0 1 2 0 4 21 6 15 – 12 19 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 7 10 7 16 17 35 38 39 – 28 14 7 24 31 20 25 11 2 2 2 3 0 1
Endoplasmic Reticulum 0 1 0 0 0 0 1 0 0 – 0 1 0 0 0 4 4 4 0 0 0 0 2 2
Endosome 0 0 2 0 1 4 2 4 0 – 0 0 0 1 1 5 3 0 1 5 1 2 7 11
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 2 3 0 0 2 0 3 1 2
Mitochondria 26 35 19 25 24 108 189 139 216 – 109 138 8 12 18 9 4 1 2 1 1 2 9 11
Nucleus 165 221 234 129 191 194 267 398 241 – 113 211 134 238 216 279 263 136 160 212 226 107 174 173
Nuclear Periphery 1 2 0 3 1 1 1 0 2 – 1 2 3 1 2 4 2 2 0 1 1 0 4 8
Nucleolus 18 13 3 6 1 9 13 18 13 – 10 16 7 17 15 24 9 6 15 16 11 11 36 52
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0
SpindlePole 0 1 5 5 15 24 24 52 12 – 4 16 0 1 0 0 6 1 0 6 0 2 6 12
Vac/Vac Membrane 0 7 2 0 2 1 3 4 3 – 2 4 1 3 9 9 6 9 3 19 20 24 68 72
Unique Cell Count 180 253 254 144 222 279 379 540 391 190 281 145 277 275 337 312 163 188 275 270 168 323 368
Labelled Cell Count 213 288 278 177 251 364 575 674 551 283 432 161 297 293 356 326 170 188 275 270 168 323 368


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 5.7 5.7 5.3 5.5 4.3 4.4 4.8 4.0 – 4.1 4.1 6.4 5.5 5.3 9.0 9.0 9.2 5.9 5.2 5.5
Std Deviation (1e-4) 0.7 1.0 1.0 1.5 1.5 1.2 1.4 1.2 1.6 – 2.2 1.1 10.5 1.3 1.4 1.4 1.6 1.5 2.7 1.6 1.4
Intensity Change (Log2) – – – -0.11 -0.06 -0.41 -0.37 -0.26 -0.5 – -0.47 -0.46 0.18 -0.06 -0.11 0.66 0.65 0.69 0.04 -0.13 -0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.4 1.6 1.1 2.5 1.7 2.8 – 4.0 0.6 0.4 2.2 3.2 1.1 2.0 1.3
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 3.0 1.3 0 0 0 0 – 0 0 -0.8 -1.5 -0.4 -2.7 -3.7 -3.2
Nucleus -0.9 -2.1 -6.5 -6.6 -6.0 -8.6 – -8.2 -5.3 0.1 -2.3 -4.4 -3.3 -2.8 -2.7
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 3.0 2.7 3.4 1.4 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 2.6 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 2.0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.3152 3.8739 3.2162 2.6803 2.4368 3.1666 2.1741 2.4516 1.9821 1.8757 2.2443 1.7479 1.7633 2.1188 1.606 1.4446 1.0503 1.4794
Actin 0.0219 0 0.001 0.0003 0.0002 0.0001 0.0159 0 0.0023 0.0004 0.0017 0.0003 0.0123 0 0.0006 0.003 0.0003 0.0005
Bud 0.0002 0 0 0 0.0008 0 0.0006 0 0.0003 0.0003 0.0002 0 0.0005 0 0.0002 0.0001 0.0001 0.0001
Bud Neck 0.011 0.0003 0.0007 0.0003 0.0006 0.0015 0.0018 0 0.0011 0.0009 0.0007 0.0008 0.003 0.0001 0.0005 0.0002 0.0034 0.0014
Bud Periphery 0.0006 0 0.0001 0 0.0014 0.0001 0.0013 0 0.0008 0.0002 0.0003 0.0001 0.0011 0 0.0007 0.0002 0.0002 0.0002
Bud Site 0.0035 0.0001 0.0002 0.0001 0.0046 0.0001 0.0031 0 0.0038 0.009 0.0008 0.0001 0.0025 0 0.0005 0.0002 0.0008 0.0002
Cell Periphery 0.0004 0 0.0001 0 0.0002 0 0.0006 0 0.0002 0.0002 0.0001 0 0.0002 0 0.0001 0.0001 0.0002 0
Cytoplasm 0.0014 0.0006 0.0008 0.0073 0.0034 0.0005 0.0028 0 0.0017 0.002 0.0054 0.0001 0.0018 0.0001 0.0093 0.0003 0.0003 0.0053
Cytoplasmic Foci 0.0075 0.0003 0.0045 0.0004 0.0063 0.0001 0.013 0 0.0015 0.003 0.0077 0.0004 0.0059 0 0.0004 0.0096 0.0013 0.0011
Eisosomes 0.0002 0 0.0003 0 0 0 0.0003 0 0 0 0.0001 0 0.0003 0 0 0 0.0001 0
Endoplasmic Reticulum 0.001 0.0001 0.001 0.0001 0.0005 0 0.0052 0 0.0019 0.0005 0.0061 0.0002 0.0011 0 0.0008 0.0005 0.0001 0.0008
Endosome 0.0117 0.0003 0.0017 0.0037 0.0159 0.0001 0.0225 0 0.0051 0.0076 0.0457 0.0013 0.0115 0 0.0032 0.0162 0.0008 0.0045
Golgi 0.0062 0 0.0009 0.0023 0.0011 0.0001 0.0073 0 0.0014 0.0022 0.0136 0.0004 0.004 0 0.0007 0.0109 0.0003 0.0013
Lipid Particles 0.0131 0.0001 0.0117 0.0035 0.0085 0.0001 0.0153 0 0.0034 0.0181 0.0268 0.0035 0.0152 0 0.0006 0.0163 0.0052 0.0029
Mitochondria 0.0054 0.0001 0.0056 0.0386 0.0024 0.0078 0.0126 0.0001 0.0027 0.0009 0.0429 0.001 0.011 0.0001 0.0038 0.0034 0.002 0.0007
None 0.0028 0.0006 0.006 0.0014 0.0173 0.0069 0.0028 0 0.0004 0.0003 0.0023 0.0001 0.0021 0 0.001 0.0001 0.0005 0.0002
Nuclear Periphery 0.0124 0.0047 0.0069 0.043 0.0061 0.0005 0.0156 0.0018 0.0129 0.0058 0.0378 0.0028 0.0085 0.0014 0.0118 0.0023 0.0045 0.0012
Nucleolus 0.0372 0.0323 0.0264 0.0187 0.0686 0.0676 0.0243 0.0106 0.0282 0.0145 0.0774 0.0474 0.0162 0.0171 0.0243 0.0152 0.1297 0.0344
Nucleus 0.8409 0.9227 0.9106 0.862 0.8364 0.9104 0.8302 0.9872 0.9253 0.9242 0.7022 0.9386 0.885 0.978 0.922 0.8823 0.7305 0.943
Peroxisomes 0.0099 0 0.0055 0.0003 0.0013 0.0001 0.0087 0 0.0003 0.0004 0.0079 0.0005 0.0077 0 0.0002 0.0371 0.0016 0.0005
Punctate Nuclear 0.0102 0.0373 0.0151 0.0163 0.0174 0.0037 0.0082 0.0002 0.0035 0.0023 0.0127 0.0019 0.0075 0.0032 0.0168 0.0008 0.1169 0.0008
Vacuole 0.0016 0.0002 0.0003 0.0007 0.0061 0.0001 0.0049 0 0.0022 0.0065 0.0051 0.0002 0.0018 0 0.0016 0.0008 0.0008 0.0006
Vacuole Periphery 0.0008 0 0.0006 0.001 0.0009 0 0.0028 0 0.001 0.0008 0.0025 0.0003 0.0008 0 0.0011 0.0005 0.0002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.5504 14.9819 12.6195 13.4291 17.0311 10.4123 12.0776 12.5728 10.7458 15.3399
Translational Efficiency 1.1319 0.693 0.8089 0.513 0.6172 0.8598 0.693 0.5229 0.6392 0.6079

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1886 1148 296 1601 1931 2429 212 710 3817 3577 508 2311

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 662.60 834.86 1014.70 917.66 757.37 831.76 972.80 926.71 710.54 832.75 997.21 920.44
Standard Deviation 90.53 115.05 140.92 127.78 99.33 118.63 110.42 113.11 106.24 117.50 130.71 123.53
Intensity Change Log 2 — 0.333396 0.614843 0.469822 — 0.135169 0.361145 0.291120 — 0.231065 0.485094 0.377268

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000105 0.000545 0.001886 0.000960 0.000184 0.000413 0.001239 0.001127 0.000145 0.000455 0.001616 0.001012
Bud Neck 0.002305 0.006984 0.010551 0.024323 0.003884 0.009545 0.002854 0.016197 0.003104 0.008723 0.007339 0.021826
Bud Site 0.001022 0.000740 0.001678 0.004691 0.000310 0.001191 0.000690 0.003795 0.000662 0.001047 0.001265 0.004415
Cell Periphery 0.000101 0.000054 0.000069 0.000122 0.000053 0.000077 0.000049 0.000085 0.000077 0.000069 0.000060 0.000110
Cytoplasm 0.025920 0.001957 0.002256 0.009465 0.008905 0.003501 0.001359 0.006457 0.017313 0.003006 0.001882 0.008541
Cytoplasmic Foci 0.005727 0.001415 0.000109 0.000869 0.000762 0.001968 0.000221 0.001980 0.003215 0.001790 0.000156 0.001211
Eisosomes 0.000029 0.000026 0.000054 0.000026 0.000018 0.000024 0.000053 0.000027 0.000023 0.000024 0.000053 0.000026
Endoplasmic Reticulum 0.000972 0.003289 0.009179 0.007722 0.002020 0.003565 0.009674 0.007645 0.001502 0.003477 0.009386 0.007698
Endosome 0.000671 0.001167 0.000346 0.002180 0.000382 0.001194 0.000264 0.002419 0.000525 0.001186 0.000312 0.002253
Golgi 0.000343 0.000789 0.000142 0.001248 0.000143 0.001392 0.000093 0.000940 0.000242 0.001199 0.000122 0.001154
Lipid Particles 0.001792 0.000971 0.000392 0.000196 0.000828 0.001015 0.000568 0.000257 0.001304 0.001001 0.000466 0.000215
Mitochondria 0.003878 0.005639 0.001377 0.003310 0.002135 0.006540 0.000768 0.016813 0.002996 0.006251 0.001123 0.007458
Mitotic Spindle 0.001099 0.005630 0.000717 0.026005 0.002424 0.007415 0.000185 0.018790 0.001770 0.006842 0.000495 0.023788
None 0.029402 0.004386 0.001855 0.005908 0.007040 0.003226 0.003352 0.006567 0.018089 0.003598 0.002480 0.006111
Nuclear Periphery 0.001217 0.000848 0.000533 0.002153 0.002149 0.001711 0.000386 0.001777 0.001688 0.001434 0.000471 0.002037
Nuclear Periphery Foci 0.001266 0.000772 0.000308 0.000731 0.001031 0.000939 0.000329 0.000356 0.001147 0.000885 0.000317 0.000615
Nucleolus 0.056446 0.049939 0.015698 0.014087 0.042289 0.047858 0.018148 0.010431 0.049284 0.048526 0.016721 0.012964
Nucleus 0.865800* 0.910906* 0.947678* 0.888437* 0.924124* 0.904376* 0.955521* 0.891462* 0.895306* 0.906472* 0.950951* 0.889366*
Peroxisomes 0.000906 0.001917 0.000636 0.000424 0.000460 0.001162 0.000537 0.000264 0.000681 0.001404 0.000595 0.000375
Vacuole 0.000695 0.000934 0.004353 0.005553 0.000484 0.002117 0.003590 0.010152 0.000588 0.001737 0.004035 0.006966
Vacuole Periphery 0.000304 0.001089 0.000186 0.001593 0.000375 0.000772 0.000118 0.002459 0.000340 0.000874 0.000158 0.001859

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -8.81 -11.47 -19.20 -10.16 5.05 -4.36 -6.41 -16.65 -13.19 -0.93 -8.10 -12.83 -24.78 -16.24 3.62
Bud Neck -7.23 -8.83 -20.49 -16.16 -9.66 -8.03 -1.61 -13.43 -9.92 -12.48 -11.12 -8.47 -24.01 -18.35 -14.86
Bud Site 0.48 -3.65 -6.01 -7.97 -3.07 -5.12 -4.49 -9.67 -7.69 -6.67 -1.78 -5.50 -10.24 -10.01 -6.00
Cell Periphery 3.04 0.26 -0.15 -4.94 -0.58 -1.22 -1.32 -5.65 -1.31 -3.50 0.54 -0.70 -2.78 -2.47 -2.33
Cytoplasm 11.98 11.87 7.89 -6.38 -6.18 4.68 7.32 1.05 -3.34 -5.77 12.07 13.71 6.84 -6.06 -8.21
Cytoplasmic Foci 5.34 7.59 7.02 1.56 -1.51 -2.76 4.03 0.72 2.85 -1.51 3.88 8.21 6.92 3.68 -2.04
Eisosomes 1.43 -5.77 -1.18 -4.14 5.66 -2.92 -7.06 -11.70 -9.71 2.26 0.19 -9.10 -7.74 -10.00 6.28
Endoplasmic Reticulum -11.20 -12.95 -23.62 -14.98 1.79 -5.20 -4.38 -14.48 -12.16 0.20 -10.41 -9.34 -25.93 -18.86 1.21
Endosome -1.20 0.09 -1.62 -0.54 -1.67 -4.17 -0.83 -3.25 -0.61 -2.56 -3.42 -0.60 -2.65 -0.73 -2.41
Golgi -1.39 1.45 -0.79 0.27 -1.16 -2.95 0.02 -1.93 2.10 -2.00 -3.10 1.08 -1.33 1.59 -1.57
Lipid Particles 2.28 3.70 5.24 2.20 3.05 -0.61 0.46 1.29 2.60 1.44 1.19 2.91 4.76 3.73 3.42
Mitochondria -0.95 2.19 0.06 1.17 -7.37 -4.24 2.82 -2.40 -0.45 -2.95 -3.37 2.99 -2.24 1.16 -4.95
Mitotic Spindle -2.64 -1.37 -7.63 -4.97 -4.96 -3.52 1.69 -5.72 -4.53 -6.10 -4.75 -0.85 -9.39 -6.78 -7.76
None 10.11 11.35 9.91 -0.92 -5.84 4.14 3.59 0.70 -2.61 -2.46 10.96 11.94 9.48 -2.92 -5.25
Nuclear Periphery 0.79 1.17 -8.43 -6.98 -12.14 1.19 4.37 -3.27 -4.63 -7.18 0.98 4.12 -7.48 -8.89 -14.11
Nuclear Periphery Foci 0.78 1.41 1.37 0.49 -0.18 1.17 0.24 0.75 -0.35 0.22 1.50 1.25 1.53 0.06 0.03
Nucleolus 3.17 11.23 12.88 9.50 -0.35 -1.44 3.30 10.90 12.83 1.89 1.91 9.96 16.06 15.01 1.08
Nucleus -7.37 -8.74 -1.80 5.75 7.30 3.66 -1.80 7.60 5.29 7.18 -3.66 -7.21 4.47 7.65 10.19
Peroxisomes -0.86 0.96 2.61 1.73 3.43 -3.60 -0.87 1.47 4.79 2.57 -2.04 0.29 2.76 3.54 3.91
Vacuole -1.27 -4.26 -9.29 -8.43 0.48 -4.37 -2.98 -6.63 -5.86 -3.18 -4.47 -5.21 -10.88 -9.20 -1.55
Vacuole Periphery -1.31 0.44 -1.79 0.33 -2.09 -1.73 1.13 -1.29 -0.39 -1.97 -2.27 1.13 -2.10 -0.04 -2.85
Standard name
Human Ortholog
Description Myb-related transcription factor; involved in regulating basal and induced expression of genes of the purine and histidine biosynthesis pathways; also involved in regulation of meiotic recombination at specific genes
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Bas1

Bas1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bas1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available