Standard name
Human Ortholog
Description High-affinity S-methylmethionine permease; required for utilization of S-methylmethionine as a sulfur source; has similarity to S-adenosylmethionine permease Sam3p
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Cytoplasm 0.97 0.94 0.97 0.95 0.96 0.88 0.82 0.8 – 0.68 0.65 0.63 1.0 0.99 1.0 – – 0.93 0.84 0.81 0.68 0.74 0.76 0.79
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Mitochondria 0 0.12 0 0.11 0.05 0.27 0.33 0.32 – 0.53 0.54 0.6 0 0 0 – – 0 0.05 0 0.17 0.12 0.11 0.06
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0.09 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 2 3 10 3 7 7
Bud 0 1 0 2 1 2 10 12 – 7 16 13 0 0 0 – – 2 1 2 5 3 8 10
Bud Neck 1 1 0 2 2 3 2 12 – 1 1 4 6 6 2 – – 1 0 1 5 0 2 3
Bud Site 0 0 0 0 0 0 1 0 – 2 0 4 0 0 0 – – 0 – – – – – –
Cell Periphery 1 0 3 4 2 0 1 1 – 1 2 2 3 3 2 – – 1 0 1 3 3 6 8
Cytoplasm 209 165 311 275 342 358 381 731 – 353 324 376 330 330 323 – – 170 190 156 214 202 368 369
Endoplasmic Reticulum 1 2 1 0 0 1 0 0 – 0 0 0 2 1 0 – – 10 1 0 5 5 3 3
Endosome 1 0 1 0 1 0 2 4 – 0 0 0 0 0 0 – – 1 3 1 1 3 4 2
Golgi 2 1 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 3 0 0 2 1 1
Mitochondria 6 22 12 31 19 108 153 290 – 273 270 361 0 0 0 – – 8 11 8 52 31 51 28
Nucleus 0 1 1 2 3 0 2 9 – 2 2 2 2 2 1 – – 1 0 0 0 0 1 0
Nuclear Periphery 0 0 0 0 1 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 2 0 0
Nucleolus 1 0 0 0 0 0 1 1 – 2 0 0 0 0 0 – – 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 1 0 1 0 – 0 0 0 0 0 0 – – 0 1 0 0 0 0 0
SpindlePole 0 0 1 2 1 4 9 22 – 9 15 13 0 0 0 – – 0 1 2 3 2 11 10
Vac/Vac Membrane 0 1 1 1 3 3 3 1 – 3 3 4 1 1 0 – – 17 5 6 3 5 6 8
Unique Cell Count 215 176 322 290 355 405 464 918 518 496 601 331 332 323 183 227 192 314 274 487 469
Labelled Cell Count 222 194 331 319 376 479 566 1083 653 633 779 344 343 328 211 227 192 314 274 487 469


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 3.8 3.6 3.6 3.6 3.0 2.7 3.0 – 2.4 2.5 2.4 5.2 5.2 5.3 4.2 4.7 5.4 5.2 5.1 5.1
Std Deviation (1e-4) 0.7 0.8 1.6 1.6 1.7 1.7 1.3 1.1 – 1.1 0.9 0.9 1.1 1.1 1.4 0.9 1.0 1.3 2.0 1.5 1.5
Intensity Change (Log2) – – – -0.0 0.02 -0.25 -0.41 -0.28 – -0.6 -0.54 -0.6 0.51 0.51 0.56 0.21 0.39 0.59 0.52 0.51 0.51


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – 0
Bud Neck – – – – – – – – – – – – – – 0
Bud Site – – – – – – – – – – – – – – 0
Cell Periphery – – – – – – – – – – – – – – 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – 0
Endosome – – – – – – – – – – – – – – 0
Golgi – – – – – – – – – – – – – – 0
Mitochondria – – – – – – – – – – – – – – 0
Nuclear Periphery – – – – – – – – – – – – – – 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – 0
Peroxisomes – – – – – – – – – – – – – – 0
SpindlePole – – – – – – – – – – – – – – 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – 0
Cytoplasm – – – – – – – – – – – – – – 0
Nucleus – – – – – – – – – – – – – – 0
Vacuole – – – – – – – – – – – – – – 5.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.4698 0.8039 0.8093 0.5409 0.4842 0.662 1.9595 2.6279 1.9954 2.5897 0.8254 2.2226 0.9874 1.4161 1.2868 1.79 2.263 1.3517
Actin 0.0158 0.0011 0.001 0.0005 0.0006 0.0076 0.0012 0.0002 0.0013 0.0296 0.0003 0.017 0.0282 0.0005 0.0152 0.0002 0.028 0.0007
Bud 0.0186 0.0332 0.026 0.0507 0.0044 0.004 0.0002 0.0002 0 0 0.0004 0 0.0001 0 0.0014 0 0.0008 0
Bud Neck 0.0005 0.0005 0.0024 0.0006 0.0006 0.0013 0.0049 0.0001 0.0003 0.0001 0 0.0001 0.0001 0 0.0003 0.0001 0.0002 0.0001
Bud Periphery 0.0093 0.0063 0.0088 0.02 0.0018 0.0021 0.0001 0.0002 0.0001 0 0.0005 0.0001 0.0002 0 0.0012 0.0001 0.0007 0
Bud Site 0.0049 0.0353 0.0269 0.0112 0.0015 0.0006 0.005 0.01 0.0002 0.0002 0.0004 0 0.0004 0.0001 0.0076 0.0004 0.001 0
Cell Periphery 0.0008 0.0008 0.0008 0.0015 0.0002 0.0002 0.0002 0.0002 0.0001 0 0 0 0.0001 0.0001 0.0001 0.0001 0.0001 0
Cytoplasm 0.0574 0.0469 0.0524 0.0434 0.0592 0.0635 0.0179 0.1097 0.1226 0.0269 0.0383 0.0895 0.0723 0.0972 0.1043 0.1175 0.0849 0.08
Cytoplasmic Foci 0.0118 0.0061 0.0173 0.0037 0.0181 0.0173 0.0816 0.003 0.0032 0.0236 0.0025 0.0025 0.0093 0.0028 0.0074 0.0024 0.0082 0.0131
Eisosomes 0.0004 0.0004 0.0002 0.0002 0.0001 0.0003 0.0001 0.0003 0.0003 0.0002 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0
Endoplasmic Reticulum 0.003 0.0011 0.0021 0.0011 0.004 0.001 0.0001 0.0022 0.0014 0.0004 0.0004 0.0008 0.0066 0.0008 0.0034 0.0029 0.0009 0.0007
Endosome 0.0057 0.0008 0.0088 0.0001 0.0013 0.001 0.0039 0.0007 0.0064 0.0006 0.0003 0.0009 0.0254 0.0029 0.006 0.0014 0.0121 0.0009
Golgi 0.0013 0.0002 0.0006 0.0001 0.0002 0.0005 0.0051 0.0001 0.0003 0.0014 0.0001 0.0004 0.0147 0.0059 0.0013 0.0001 0.0621 0.001
Lipid Particles 0.0013 0.0004 0.001 0.0002 0.0008 0.0006 0.0039 0.0004 0.0005 0.0026 0.0002 0.0003 0.0059 0.0009 0.0022 0.0002 0.0004 0.0056
Mitochondria 0.002 0.0009 0.0013 0.0005 0.0008 0.0027 0.0005 0.0002 0.0008 0.0002 0.0003 0.0001 0.0019 0.0144 0.0009 0.0003 0.0201 0.0004
None 0.8417 0.8412 0.8079 0.8236 0.8767 0.8463 0.8122 0.8706 0.8401 0.893 0.9542 0.8869 0.8032 0.8722 0.838 0.8641 0.7786 0.8936
Nuclear Periphery 0.0067 0.001 0.003 0.0017 0.0012 0.0024 0.0001 0.0004 0.0174 0.0001 0.0002 0.0001 0.0227 0.0002 0.001 0.0032 0.0002 0.0002
Nucleolus 0.0013 0.002 0.0031 0.0025 0.0022 0.0029 0.0015 0.0001 0.0002 0 0.0002 0 0.0001 0 0.0001 0.0001 0 0
Nucleus 0.0088 0.0102 0.0119 0.0198 0.0063 0.0191 0.0011 0.0005 0.0021 0.0001 0.0003 0.0001 0.0016 0.0001 0.0006 0.0053 0.0002 0.0001
Peroxisomes 0.0017 0.0003 0.0005 0.0001 0.0005 0.0004 0.0564 0.0001 0.0002 0.0199 0.0002 0.0005 0.0019 0.0012 0.0072 0.0001 0.0006 0.003
Punctate Nuclear 0.0037 0.0105 0.0217 0.0152 0.0181 0.0251 0.0034 0.0005 0.001 0.0006 0.0009 0.0002 0.0007 0.0001 0.0005 0.0005 0.0001 0.0004
Vacuole 0.0024 0.0008 0.0019 0.0031 0.0011 0.0007 0.0008 0.0005 0.0006 0.0001 0.0002 0.0003 0.0017 0.0003 0.0008 0.0009 0.0004 0.0001
Vacuole Periphery 0.001 0.0001 0.0004 0.0003 0.0002 0.0002 0 0 0.001 0 0 0 0.0026 0.0001 0.0002 0.0002 0.0002 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 28.026 10.697 42.6126 50.5558 14.2898 13.8479 11.385 17.2892 34.7957 16.3733
Translational Efficiency 0.5796 0.8604 0.6586 0.5682 0.6321 0.9269 0.6516 0.6875 0.5832 0.5713

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
94 658 1522 1587 206 1807 2288 1706 300 2465 3810 3293

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 926.80 753.44 750.86 795.67 651.09 718.04 784.44 801.90 737.48 727.49 771.03 798.90
Standard Deviation 140.10 97.84 84.61 114.75 69.87 90.31 95.40 100.72 160.80 93.70 92.72 107.75
Intensity Change Log 2 — -0.298765 -0.303714 -0.220088 — 0.141207 0.268806 0.300565 — -0.100729 -0.039476 0.017883

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.003488 0.000349 0.000049 0.001057 0.000400 0.000335 0.000173 0.000421 0.001367 0.000338 0.000123 0.000728
Bud Neck 0.014557 0.032244 0.009900 0.008875 0.032095 0.048498 0.017384 0.007788 0.026600 0.044159 0.014394 0.008312
Bud Site 0.041263 0.009543 0.008641 0.033986 0.002977 0.014774 0.026359 0.035665 0.014973 0.013377 0.019281 0.034856
Cell Periphery 0.000481 0.000310 0.000102 0.000232 0.000239 0.000189 0.000148 0.000272 0.000315 0.000221 0.000129 0.000253
Cytoplasm 0.227964* 0.371373* 0.481420* 0.469785* 0.327811* 0.382793* 0.527500* 0.496352* 0.296526* 0.379745* 0.509093* 0.483548*
Cytoplasmic Foci 0.075006 0.268815* 0.012423 0.022052 0.354571* 0.270544* 0.007042 0.022142 0.266974* 0.270082* 0.009192 0.022099
Eisosomes 0.000155 0.000176 0.000022 0.000050 0.000582 0.000267 0.000023 0.000045 0.000448 0.000243 0.000022 0.000047
Endoplasmic Reticulum 0.002724 0.000829 0.000578 0.001881 0.007636 0.000520 0.000767 0.001842 0.006097 0.000602 0.000691 0.001861
Endosome 0.028486 0.028147 0.001684 0.006585 0.032646 0.028964 0.002074 0.008232 0.031342 0.028746 0.001918 0.007438
Golgi 0.004303 0.002761 0.000134 0.005039 0.005205 0.006205 0.000359 0.004767 0.004922 0.005285 0.000269 0.004899
Lipid Particles 0.004244 0.010311 0.000536 0.001482 0.043299 0.011801 0.000538 0.000969 0.031061 0.011403 0.000538 0.001216
Mitochondria 0.001580 0.004874 0.001300 0.002941 0.007639 0.014578 0.002088 0.004932 0.005740 0.011988 0.001773 0.003972
Mitotic Spindle 0.013069 0.004757 0.009132 0.042030 0.000186 0.005953 0.018247 0.021093 0.004223 0.005634 0.014606 0.031183
None 0.001182 0.006158 0.005493 0.004643 0.000622 0.002375 0.008083 0.008887 0.000798 0.003385 0.007049 0.006841
Nuclear Periphery 0.000841 0.000829 0.001590 0.001597 0.000723 0.000416 0.001615 0.001173 0.000760 0.000526 0.001605 0.001378
Nuclear Periphery Foci 0.001482 0.000458 0.000106 0.000897 0.000598 0.000190 0.000152 0.000431 0.000875 0.000261 0.000133 0.000655
Nucleolus 0.000549 0.002096 0.000555 0.000514 0.000621 0.001556 0.000943 0.000749 0.000598 0.001700 0.000788 0.000636
Nucleus 0.211861* 0.130299* 0.435520* 0.288494* 0.090978 0.107046 0.347089* 0.287675* 0.128854 0.113253 0.382415* 0.288070*
Peroxisomes 0.003162 0.021275 0.000631 0.001018 0.019800 0.017127 0.000330 0.001158 0.014587 0.018235 0.000450 0.001090
Vacuole 0.361346* 0.102506 0.029627 0.103482 0.070258 0.082619 0.037794 0.092276 0.161466 0.087928 0.034531 0.097677
Vacuole Periphery 0.002257 0.001892 0.000557 0.003362 0.001113 0.003250 0.001291 0.003132 0.001471 0.002888 0.000998 0.003243

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.41 1.65 1.21 -1.47 -3.48 0.53 2.11 1.04 0.65 -2.24 1.49 2.02 1.24 -1.06 -3.87
Bud Neck -4.08 -0.08 0.30 8.12 1.36 -2.23 2.40 3.57 16.11 6.40 -3.52 2.35 3.45 17.87 5.94
Bud Site 2.16 2.40 0.74 -5.05 -6.11 -7.71 -8.09 -7.40 -1.77 -0.43 -0.84 -1.01 -3.06 -3.79 -3.44
Cell Periphery 0.49 2.50 1.85 0.90 -5.41 1.55 4.01 1.65 0.64 -1.22 1.18 4.19 2.60 1.18 -2.86
Cytoplasm -5.89 -8.87 -6.68 -1.00 5.40 -3.21 -6.64 -4.75 -2.89 3.33 -5.91 -10.21 -7.18 -2.32 6.29
Cytoplasmic Foci -17.07 5.99 5.50 29.09 -3.33 5.30 23.32 22.52 47.76 -9.70 -0.47 19.32 18.67 56.23 -10.01
Eisosomes -0.92 8.76 6.35 8.80 -17.31 4.15 7.60 7.24 10.05 -15.19 3.82 8.19 7.60 11.62 -22.80
Endoplasmic Reticulum 4.74 4.95 0.56 -11.36 -13.82 2.26 2.03 1.81 -4.24 -2.01 2.58 2.37 1.78 -9.28 -6.83
Endosome -1.52 3.83 3.35 8.88 -3.54 0.88 7.05 6.36 17.73 -4.71 0.05 7.97 7.20 19.98 -5.83
Golgi -0.15 1.79 0.24 0.64 -2.45 -0.90 5.20 2.70 4.69 -2.72 -1.02 5.36 2.43 4.68 -3.62
Lipid Particles -4.18 5.73 4.23 6.60 -9.03 4.70 6.42 6.35 14.86 -7.33 4.15 6.49 6.35 15.88 -11.47
Mitochondria -4.77 -0.35 -0.90 4.49 -0.89 -1.92 1.72 1.46 8.70 -1.03 -2.54 1.71 1.45 10.08 -1.25
Mitotic Spindle 0.41 -0.17 -3.17 -6.85 -5.88 -4.82 -8.59 -7.83 -4.20 -0.89 -1.52 -4.10 -7.55 -8.01 -4.96
None -2.46 -4.59 -5.12 1.05 1.38 -4.32 -8.13 -5.96 -3.70 0.50 -4.18 -9.46 -8.23 -2.08 1.33
Nuclear Periphery 1.21 -5.74 -8.43 -9.27 -4.18 2.64 -11.75 -9.85 -20.60 3.72 3.05 -13.06 -13.34 -21.43 -0.30
Nuclear Periphery Foci 4.02 5.50 2.17 -4.94 -11.03 3.58 3.62 0.83 -6.28 -6.22 5.40 6.37 1.38 -9.49 -12.41
Nucleolus -5.43 -1.60 -0.96 5.26 0.90 -6.32 -1.46 -0.60 5.06 0.63 -8.41 -1.81 -0.64 7.89 1.11
Nucleus 2.31 -9.77 -4.92 -22.26 13.64 -1.89 -33.58 -24.07 -31.84 10.24 0.93 -27.93 -18.44 -40.43 16.54
Peroxisomes -7.18 2.60 2.72 8.02 -0.11 0.59 4.60 4.49 13.12 -2.72 -1.23 4.71 4.61 15.38 -1.87
Vacuole 9.71 12.09 7.48 -8.76 -23.97 -1.58 1.78 -10.88 -14.02 -19.03 6.24 9.00 0.63 -18.04 -29.86
Vacuole Periphery -0.55 1.71 -0.01 0.71 -2.42 -4.67 0.79 -0.69 3.17 -1.15 -4.26 1.85 -0.61 3.35 -2.20
Standard name
Human Ortholog
Description High-affinity S-methylmethionine permease; required for utilization of S-methylmethionine as a sulfur source; has similarity to S-adenosylmethionine permease Sam3p
Localization
Cell Percentages ER (10%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Mmp1

Mmp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mmp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available