Standard name
Human Ortholog
Description Water channel that mediates water transport across cell membranes; only expressed in proliferating cells; controlled by osmotic signals; may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.05 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.93 0.91 0.97 0.87 0.76 0.77 0.7 0.67 0.62 0.63 0.71 0.84 0.95 0.91 0.91 0.88 0.86 0.74 0.75 0.7 0.74 0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.17 0.08 0 0 0 0.07 0 0.05 0.06 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.1 0.1 0.06 0.2 0.37 0.27 0.39 0.29 0.43 0.46 0 0 0 0 0 0 0.05 0.09 0.09 0.09 0.08 0.07
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0.06 0.09 0.06 0 0.1 0.08 0 0 0 0.06 0 0 0 0.05 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 0 1 0 1 2 0 6 3 0 0 2 3 0 6 1 2 5 5
Bud 0 2 1 3 8 4 7 10 19 11 24 22 3 2 0 0 0 2 0 3 1 5 6 10
Bud Neck 0 0 1 0 3 3 5 1 7 1 2 1 0 2 5 1 2 3 1 4 0 1 6 3
Bud Site 0 0 0 0 0 0 0 0 0 1 3 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 1 0 0 0 2 2 6 1 8 8 3 6 7 5 2 4 7 0 2 0 1 6 6
Cytoplasm 79 240 99 186 439 225 330 329 249 327 257 304 235 441 631 210 145 145 89 202 85 120 277 427
Endoplasmic Reticulum 1 6 0 4 0 1 11 4 6 15 9 16 56 41 27 7 7 12 2 12 6 6 7 2
Endosome 0 0 0 1 1 0 1 4 2 1 3 1 0 3 0 7 1 1 0 3 0 3 3 2
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 1 3
Mitochondria 1 3 11 20 27 52 160 115 138 142 179 222 2 2 1 8 3 1 5 23 9 16 28 35
Nucleus 0 0 0 0 2 0 2 4 6 4 4 4 3 0 2 1 3 0 0 0 0 0 0 1
Nuclear Periphery 0 0 0 2 1 0 2 0 0 9 5 6 1 1 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 1 0 0 0 0 3 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 3 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 2 3 4 1 8 3 1 5 1 0 0 1 0 0 2 0 2 4 4
Vac/Vac Membrane 0 0 1 3 4 0 4 19 20 42 23 17 32 40 6 9 4 10 2 9 2 9 17 19
Unique Cell Count 81 247 106 205 452 258 433 430 356 489 417 479 329 524 663 230 159 165 105 273 114 172 377 535
Labelled Cell Count 83 252 113 220 486 290 527 497 449 573 525 597 350 544 677 246 172 184 105 273 114 172 377 535


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 4.2 3.5 4.3 3.6 3.3 3.4 3.9 3.7 4.1 3.9 3.9 7.3 6.3 5.4 5.4 5.6 6.3 5.4 5.1 5.0
Std Deviation (1e-4) 0.4 0.8 1.1 2.4 1.6 1.8 1.7 1.7 1.5 1.6 1.7 1.4 3.0 2.2 1.2 2.1 2.0 2.6 1.5 1.5 1.3
Intensity Change (Log2) – – – 0.28 0.02 -0.08 -0.06 0.15 0.08 0.21 0.15 0.13 1.06 0.85 0.62 0.63 0.68 0.84 0.62 0.52 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.0148 0.2083 -0.0272 -0.1411 -0.7219 0.176 0.3336 -0.301 -0.1634 -0.2882 -0.5144 -0.2537 -2.2698 -1.8175 -1.7425 -1.6834 -1.7907 -1.8671
Actin 0.0027 0.0009 0.0012 0.0001 0.0001 0.0001 0.0312 0.0001 0.0004 0.0323 0.0134 0.0016 0.0085 0.0001 0.0034 0.0001 0.0003 0.0003
Bud 0.0002 0.0007 0.0009 0 0 0 0.0003 0 0.0055 0.0001 0.0002 0.0002 0.0002 0 0.0001 0.0001 0.0098 0
Bud Neck 0.0011 0.0071 0.0005 0 0 0.0004 0.009 0 0.0001 0.0003 0.0005 0.0001 0.0019 0 0.0001 0 0.0002 0.0002
Bud Periphery 0.0006 0.001 0.0021 0 0 0 0.0007 0 0.0016 0.0002 0.0004 0.0009 0.0004 0 0.0003 0.0001 0.0256 0.0001
Bud Site 0.0005 0.0106 0.0011 0 0 0 0.0134 0 0.0084 0.0005 0.001 0.0001 0.0008 0.0002 0.0008 0 0.0024 0
Cell Periphery 0.0002 0.0003 0.0001 0 0 0 0.0021 0.0001 0.0003 0.0001 0.0001 0.0002 0.0002 0.0001 0.0003 0.0001 0.0006 0
Cytoplasm 0.0878 0.0702 0.0473 0.0759 0.1179 0.1242 0.0172 0.0143 0.0259 0.0091 0.0127 0.0414 0.1075 0.1136 0.1454 0.1126 0.1621 0.1966
Cytoplasmic Foci 0.0044 0.0059 0.0092 0.0021 0.0056 0.0065 0.0401 0.0008 0.0045 0.0039 0.0161 0.0131 0.0054 0.0079 0.0071 0.0139 0.0052 0.0141
Eisosomes 0.0002 0.0002 0.0001 0 0 0 0.0012 0.0009 0.0009 0.0004 0.0002 0.0001 0.0002 0.0001 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0078 0.0094 0.0007 0.0004 0.0007 0.0046 0.0066 0.0003 0.0009 0.0002 0.0004 0.0089 0.0197 0.0023 0.0101 0.0052 0.0032 0.0201
Endosome 0.006 0.0005 0.0016 0.0004 0.002 0.001 0.0322 0.0001 0.002 0.0018 0.0126 0.0048 0.0068 0.0031 0.0054 0.0154 0.0021 0.0089
Golgi 0.0006 0.0001 0.0004 0 0.0001 0.0001 0.0066 0.0001 0.0006 0.0053 0.0191 0.0013 0.0022 0.0001 0.0009 0.0003 0.0001 0.0007
Lipid Particles 0.0009 0.0002 0.0002 0 0.0007 0.0004 0.0264 0.0003 0.0099 0.0046 0.0463 0.0098 0.0026 0.0006 0.0021 0.0008 0.0002 0.0003
Mitochondria 0.0006 0.0003 0.0014 0.0001 0.0001 0.0001 0.0025 0.0001 0.001 0.0009 0.011 0.0038 0.0006 0.0001 0.0004 0.0009 0.0006 0.0003
None 0.8731 0.8879 0.9257 0.9197 0.8717 0.8598 0.7188 0.9821 0.9319 0.9361 0.8502 0.9036 0.836 0.8688 0.8122 0.8416 0.765 0.7553
Nuclear Periphery 0.0054 0.0004 0.0003 0.0001 0.0002 0.0006 0.0131 0.0001 0.0003 0.0001 0.0002 0.0016 0.0025 0.0004 0.003 0.0012 0.0015 0.0008
Nucleolus 0.0003 0.0006 0.0003 0 0 0.0001 0.0018 0.0001 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0003 0.0001
Nucleus 0.0041 0.0009 0.0006 0.0002 0.0003 0.0007 0.0038 0.0001 0.0006 0.0001 0.0001 0.0008 0.001 0.0003 0.001 0.0006 0.0016 0.0004
Peroxisomes 0.0002 0.0004 0.0048 0.0001 0 0.0002 0.0453 0 0.0034 0.0035 0.0138 0.0023 0.0009 0.0001 0.0036 0.0001 0.0001 0.0002
Punctate Nuclear 0.0014 0.0017 0.0007 0.0003 0.0002 0.001 0.0254 0.0002 0.0004 0.0003 0.0007 0.0039 0.001 0.0003 0.0014 0.0004 0.0003 0.0007
Vacuole 0.0017 0.0006 0.0007 0.0001 0.0002 0.0003 0.0015 0.0001 0.0011 0.0001 0.0004 0.0009 0.0012 0.0019 0.0017 0.0051 0.0143 0.0008
Vacuole Periphery 0.0003 0.0001 0.0001 0 0 0 0.0009 0 0.0002 0.0001 0.0003 0.0003 0.0002 0.0001 0.0005 0.0013 0.0042 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.356 6.5758 4.8796 8.4812 11.127 14.7039 6.6488 5.2354 8.1986 9.2556
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2025 605 2953 705 1118 435 2371 1872 3143 1040 5324 2577

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 567.02 772.43 1013.41 1188.07 681.54 679.21 993.69 1249.78 607.76 733.44 1004.63 1232.90
Standard Deviation 96.79 134.11 324.83 540.23 118.55 107.54 314.84 485.78 118.49 131.96 320.57 502.02
Intensity Change Log 2 — 0.446005 0.837746 1.067148 — -0.004941 0.543998 0.874804 — 0.217419 0.684847 0.965344

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000121 0.001305 0.002365 0.003475 0.000414 0.003739 0.002025 0.003252 0.000225 0.002323 0.002213 0.003313
Bud Neck 0.023122 0.024621 0.013251 0.017659 0.010209 0.051240 0.016262 0.013810 0.018529 0.035755 0.014592 0.014863
Bud Site 0.003503 0.029711 0.037559 0.089751 0.007949 0.026230 0.044452 0.073720 0.005085 0.028255 0.040629 0.078106
Cell Periphery 0.000277 0.000551 0.004220 0.024443 0.000621 0.000468 0.006380 0.015200 0.000400 0.000516 0.005182 0.017729
Cytoplasm 0.436777* 0.340922* 0.238671* 0.163242* 0.421536* 0.278492* 0.304587* 0.202881* 0.431355* 0.314809* 0.268026* 0.192037*
Cytoplasmic Foci 0.254398* 0.175740 0.008928 0.027659 0.217045* 0.298647* 0.013276 0.025833 0.241111* 0.227149* 0.010865 0.026332
Eisosomes 0.000466 0.000283 0.000230 0.000164 0.000350 0.000756 0.000205 0.000147 0.000424 0.000481 0.000219 0.000152
Endoplasmic Reticulum 0.003532 0.082560 0.457453* 0.190167* 0.102929* 0.038118 0.344690* 0.293951* 0.038889 0.063971 0.407235* 0.265559*
Endosome 0.006824 0.029390 0.007603 0.052469 0.020193 0.031285 0.009090 0.040038 0.011580 0.030183 0.008265 0.043439
Golgi 0.004741 0.005805 0.000473 0.014403 0.003798 0.010084 0.000812 0.007525 0.004405 0.007595 0.000624 0.009407
Lipid Particles 0.019202 0.015957 0.007236 0.048900 0.032803 0.023146 0.009757 0.040010 0.024040 0.018964 0.008359 0.042442
Mitochondria 0.016485 0.009219 0.003112 0.002611 0.005108 0.026315 0.003848 0.003092 0.012438 0.016370 0.003440 0.002961
Mitotic Spindle 0.000236 0.007671 0.002617 0.013016 0.000580 0.007136 0.004628 0.006174 0.000359 0.007447 0.003512 0.008046
None 0.005548 0.001799 0.002657 0.000928 0.004019 0.001109 0.003502 0.002539 0.005004 0.001510 0.003033 0.002098
Nuclear Periphery 0.000848 0.002099 0.005589 0.001152 0.002564 0.001169 0.006925 0.001183 0.001459 0.001710 0.006184 0.001175
Nuclear Periphery Foci 0.000378 0.004883 0.010799 0.015194 0.004325 0.001190 0.011195 0.012041 0.001782 0.003339 0.010975 0.012904
Nucleolus 0.001330 0.001106 0.000185 0.000299 0.001141 0.001091 0.000328 0.000353 0.001263 0.001099 0.000249 0.000338
Nucleus 0.189239* 0.071155 0.108097 0.016383 0.091583 0.078347 0.106203 0.020335 0.154501* 0.074164 0.107254 0.019254
Peroxisomes 0.008331 0.010689 0.000610 0.002364 0.004002 0.035067 0.000522 0.001036 0.006791 0.020886 0.000570 0.001399
Vacuole 0.023328 0.179913 0.087721 0.308549* 0.067230 0.083319 0.110186 0.232005* 0.038944 0.139511 0.097725 0.252945*
Vacuole Periphery 0.001315 0.004621 0.000626 0.007170 0.001599 0.003052 0.001127 0.004872 0.001416 0.003965 0.000849 0.005501

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.13 -11.30 -6.32 -3.68 -1.72 -2.13 -7.12 -8.65 0.36 -3.12 -3.08 -13.59 -11.44 -1.31 -3.20
Bud Neck -0.21 5.57 3.33 2.78 -1.62 -7.50 -4.25 -2.79 6.84 1.65 -5.92 3.14 3.12 7.54 0.16
Bud Site -8.16 -15.69 -12.30 -7.77 -6.68 -4.26 -12.18 -16.69 -8.71 -6.94 -8.96 -20.33 -21.93 -12.18 -10.04
Cell Periphery -2.24 -12.53 -7.97 -7.85 -6.22 1.41 -7.67 -10.67 -10.81 -5.51 -1.30 -12.94 -13.45 -13.33 -8.80
Cytoplasm 7.69 25.18 20.15 8.95 4.29 9.99 10.03 17.86 3.11 9.45 12.37 24.98 28.54 8.70 8.17
Cytoplasmic Foci 9.38 52.18 43.44 18.55 -7.79 -6.61 33.78 31.15 24.70 -7.12 2.06 61.74 55.10 29.64 -11.36
Eisosomes 4.30 5.42 7.46 3.38 2.00 -1.72 4.25 6.54 2.67 2.33 -0.47 6.96 10.45 3.41 3.21
Endoplasmic Reticulum -8.18 -54.28* -12.73 -4.36 21.60 6.21 -17.94 -12.90 -18.97 4.60 -3.28 -52.14 -24.39 -17.21 16.98
Endosome -9.36 -1.18 -9.75 -3.10 -9.58 -3.74 8.00 -7.01 -1.44 -14.76 -9.95 5.40 -15.09 -3.35 -17.97
Golgi -0.78 12.32 -2.87 -2.36 -5.15 -3.34 8.56 -2.89 1.80 -6.74 -3.14 14.72 -3.64 -0.11 -8.58
Lipid Particles 1.83 10.78 -4.22 -4.79 -7.17 2.88 10.72 -0.91 -3.50 -10.42 2.95 14.83 -5.22 -6.67 -13.08
Mitochondria 3.22 8.74 6.98 2.75 0.69 -4.98 1.22 1.85 5.68 1.42 -1.51 8.43 8.39 6.30 1.32
Mitotic Spindle -2.92 -6.07 -4.98 -1.99 -3.99 -2.21 -5.44 -6.58 -0.22 -1.99 -3.68 -8.16 -8.33 -1.11 -4.80
None 4.50 3.81 5.95 0.98 2.99 3.64 0.82 1.92 -2.59 1.57 5.82 3.63 4.91 -1.46 1.92
Nuclear Periphery -2.81 -11.04 -1.87 1.85 10.04 2.93 -5.15 3.58 -0.23 7.55 -0.76 -10.99 1.15 1.58 12.20
Nuclear Periphery Foci -4.49 -17.14 -8.95 -5.06 -1.69 5.42 -7.24 -7.17 -11.64 -0.55 -2.38 -17.91 -13.53 -9.48 -1.33
Nucleolus 0.62 4.23 3.56 3.36 -3.51 0.23 3.55 3.02 2.75 -0.52 0.66 5.30 4.29 4.04 -1.55
Nucleus 18.31 12.77 30.43 7.44 19.68 2.01 -6.37 16.57 8.19 24.12 16.56 8.47 36.83 11.67 32.41
Peroxisomes -1.59 11.84 7.28 6.75 -2.12 -8.25 10.26 8.69 9.09 -3.45 -7.80 14.31 12.05 11.17 -3.20
Vacuole -19.26 -26.95 -27.09 -10.45 -19.85 -2.53 -13.71 -26.68 -19.51 -16.81 -17.97 -29.85 -41.24 -15.98 -26.64
Vacuole Periphery -3.06 4.87 -5.24 -0.86 -6.24 -3.30 3.05 -6.74 -2.23 -8.34 -3.94 5.25 -8.87 -1.15 -10.94
Standard name
Human Ortholog
Description Water channel that mediates water transport across cell membranes; only expressed in proliferating cells; controlled by osmotic signals; may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains
Localization
Cell Percentages cytoplasm (28%), ER (17%), mixed (28%)
Cell Cycle Regulation Yes
cytoplasm - S/G2 (0.00075)
Subcompartmental Group N/A

Aqy2

Aqy2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aqy2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available