Standard name | |
---|---|
Human Ortholog | |
Description | ER membrane localized phosphoryltransferase; adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 12 | 2 | 22 | 19 | 16 | 10 | 6 | 13 | 5 | – | 8 | 9 | 16 | 28 | 37 | 16 | 2 | 11 | 4 | 5 | 6 | 6 | 7 | 8 |
Bud | 1 | 0 | 2 | 11 | 4 | 2 | 6 | 13 | 3 | – | 7 | 12 | 5 | 1 | 5 | 1 | 0 | 0 | 1 | 0 | 1 | 3 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 12 | 3 | 7 | 17 | 12 | 17 | 16 | 16 | 18 | – | 14 | 13 | 24 | 58 | 73 | 26 | 16 | 24 | 0 | 0 | 3 | 1 | 1 | 3 |
Cytoplasm | 19 | 29 | 47 | 59 | 61 | 83 | 121 | 124 | 71 | – | 71 | 93 | 50 | 61 | 86 | 87 | 49 | 42 | 4 | 7 | 5 | 7 | 6 | 7 |
Endoplasmic Reticulum | 182 | 114 | 291 | 166 | 197 | 153 | 136 | 178 | 152 | – | 105 | 87 | 167 | 304 | 274 | 5 | 0 | 3 | 220 | 142 | 416 | 158 | 246 | 278 |
Endosome | 1 | 0 | 2 | 1 | 8 | 3 | 12 | 3 | 0 | – | 1 | 2 | 1 | 1 | 1 | 5 | 1 | 1 | 2 | 4 | 1 | 1 | 2 | 2 |
Golgi | 4 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 6 | 12 | 4 | 1 | 4 | 4 | 0 | 3 | 4 |
Mitochondria | 2 | 10 | 21 | 104 | 67 | 135 | 140 | 202 | 178 | – | 172 | 183 | 11 | 8 | 9 | 7 | 2 | 4 | 6 | 6 | 23 | 7 | 10 | 11 |
Nucleus | 0 | 0 | 0 | 0 | 2 | 3 | 6 | 10 | 7 | – | 3 | 7 | 0 | 1 | 1 | 2 | 5 | 2 | 0 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 9 | 7 | 7 | 3 | 4 | 6 | 8 | 5 | 12 | – | 15 | 5 | 5 | 4 | 8 | 3 | 0 | 1 | 3 | 6 | 5 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 3 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | – | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 48 | 16 | 50 | 25 | 47 | 29 | 108 | 59 | 32 | – | 18 | 55 | 13 | 18 | 23 | 57 | 55 | 15 | 4 | 10 | 10 | 12 | 11 | 19 |
Unique Cell Count | 236 | 157 | 389 | 307 | 334 | 355 | 463 | 500 | 368 | 311 | 357 | 238 | 399 | 407 | 198 | 132 | 100 | 252 | 192 | 481 | 206 | 299 | 344 | |
Labelled Cell Count | 290 | 182 | 449 | 406 | 420 | 442 | 560 | 626 | 478 | 414 | 472 | 294 | 484 | 517 | 216 | 143 | 108 | 252 | 192 | 481 | 206 | 299 | 344 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.4 | 8.2 | 6.9 | 6.2 | 6.7 | 6.1 | 6.2 | 5.8 | 5.3 | – | 5.3 | 5.1 | 8.4 | 8.5 | 7.5 | 10.4 | 11.7 | 11.5 | 8.5 | 8.5 | 8.9 |
Std Deviation (1e-4) | 1.5 | 1.5 | 1.0 | 0.9 | 1.3 | 1.1 | 1.2 | 1.0 | 1.0 | – | 1.0 | 1.0 | 1.5 | 1.3 | 1.1 | 1.8 | 1.9 | 2.1 | 1.7 | 1.5 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.14 | -0.04 | -0.17 | -0.14 | -0.24 | -0.38 | – | -0.39 | -0.43 | 0.28 | 0.3 | 0.11 | 0.59 | 0.76 | 0.74 | 0.3 | 0.3 | 0.37 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.3 | -0.5 | -1.9 | -3.6 | -2.3 | -3.2 | – | -2.0 | -2.1 | 0.5 | 0.8 | 1.8 | 1.1 | -2.0 | 1.9 |
Bud | 3.0 | 0 | 0 | 0 | 2.4 | 0 | – | 0 | 2.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 2.7 | 1.5 | 2.3 | 1.5 | 1.3 | 2.4 | – | 2.1 | 1.6 | 4.6 | 6.5 | 7.6 | 5.6 | 5.0 | 8.1 |
Cytoplasm | 2.6 | 2.3 | 4.1 | 5.1 | 4.8 | 2.7 | – | 3.8 | 4.9 | 3.0 | 1.3 | 3.4 | 8.7 | 6.4 | 6.9 |
Endoplasmic Reticulum | -5.7 | -4.5 | -8.8 | -13.2 | -11.6 | -9.4 | – | -10.9 | -13.8 | -1.3 | 0.5 | -2.3 | -16.6 | -15.0 | -13.1 |
Endosome | 0 | 0 | 0 | 2.4 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 9.7 | 6.0 | 10.9 | 9.2 | 11.9 | 13.4 | – | 14.7 | 14.0 | -0.4 | -2.5 | -2.4 | -1.0 | -1.9 | -0.6 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | -0.7 | -0.1 | -0.1 | -1.0 | 1.3 | – | 2.3 | -0.4 | 0 | -1.0 | 0.2 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -2.0 | 0.5 | -2.1 | 3.9 | -0.5 | -1.8 | – | -3.1 | 1.0 | -3.0 | -4.2 | -3.5 | 4.7 | 7.1 | 0.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.6792 | 7.296 | 6.9124 | 6.5486 | 5.4791 | 7.0017 | 4.005 | 4.5453 | 4.508 | 4.7694 | 4.1605 | 4.4629 | 9.8151 | 10.6853 | 10.0032 | 9.9803 | 10.35 | 10.227 |
Actin | 0.0311 | 0.0006 | 0.0155 | 0.0345 | 0.0301 | 0.0057 | 0.0247 | 0.0106 | 0.0042 | 0.0004 | 0.0007 | 0.0021 | 0.001 | 0.0006 | 0.0012 | 0.0006 | 0.0049 | 0.0005 |
Bud | 0.0002 | 0.0001 | 0.0004 | 0.0029 | 0.0004 | 0.0004 | 0.0004 | 0.0005 | 0.0003 | 0 | 0 | 0.0001 | 0.0002 | 0.0016 | 0.0011 | 0 | 0.0001 | 0.0002 |
Bud Neck | 0.0022 | 0.0006 | 0.0108 | 0.0013 | 0.0015 | 0.002 | 0.001 | 0.0009 | 0.0011 | 0.0002 | 0.0003 | 0.0024 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0004 |
Bud Periphery | 0.0003 | 0.0002 | 0.0012 | 0.0041 | 0.001 | 0.0011 | 0.0006 | 0.0009 | 0.0005 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0006 | 0 | 0.0001 | 0.0001 |
Bud Site | 0.0011 | 0.0008 | 0.0039 | 0.0112 | 0.0019 | 0.0004 | 0.0013 | 0.0077 | 0.0014 | 0.0001 | 0.0001 | 0.0001 | 0.0019 | 0.0031 | 0.0046 | 0 | 0.0003 | 0.0002 |
Cell Periphery | 0.0013 | 0.0024 | 0.0024 | 0.001 | 0.0006 | 0.0005 | 0.0004 | 0.0017 | 0.0004 | 0.0005 | 0.0002 | 0.0002 | 0.0004 | 0.001 | 0.0007 | 0.0002 | 0.0002 | 0.0003 |
Cytoplasm | 0.0386 | 0.085 | 0.0425 | 0.0078 | 0.0136 | 0.0241 | 0.049 | 0.0586 | 0.0241 | 0.0377 | 0.0344 | 0.0349 | 0.013 | 0.0345 | 0.0112 | 0.0074 | 0.0124 | 0.0289 |
Cytoplasmic Foci | 0.0036 | 0.0023 | 0.004 | 0.0054 | 0.0072 | 0.0015 | 0.0065 | 0.0062 | 0.0021 | 0.0037 | 0.0015 | 0.0038 | 0.0014 | 0.0019 | 0.002 | 0.0002 | 0.01 | 0.0018 |
Eisosomes | 0.0002 | 0 | 0.0003 | 0.0005 | 0.0011 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.7945 | 0.8608 | 0.8409 | 0.7923 | 0.5993 | 0.8789 | 0.7156 | 0.8203 | 0.8995 | 0.9064 | 0.8784 | 0.8669 | 0.9376 | 0.9049 | 0.946 | 0.9788 | 0.8954 | 0.929 |
Endosome | 0.0151 | 0.0079 | 0.0141 | 0.0173 | 0.0402 | 0.0176 | 0.0488 | 0.0249 | 0.0182 | 0.0125 | 0.0271 | 0.014 | 0.006 | 0.006 | 0.0041 | 0.0021 | 0.0212 | 0.0087 |
Golgi | 0.0065 | 0.001 | 0.0051 | 0.0052 | 0.0263 | 0.0058 | 0.0106 | 0.0084 | 0.0039 | 0.002 | 0.0105 | 0.0016 | 0.0021 | 0.0018 | 0.0017 | 0.0003 | 0.0139 | 0.0004 |
Lipid Particles | 0.0053 | 0.0092 | 0.0068 | 0.0132 | 0.0346 | 0.0048 | 0.0189 | 0.0102 | 0.0047 | 0.0021 | 0.0103 | 0.0015 | 0.0032 | 0.0024 | 0.0018 | 0.0005 | 0.0107 | 0.0021 |
Mitochondria | 0.0039 | 0.0003 | 0.0058 | 0.0291 | 0.0897 | 0.0076 | 0.0086 | 0.0023 | 0.0023 | 0.0015 | 0.0101 | 0.0023 | 0.0011 | 0.0004 | 0.0006 | 0.0001 | 0.0042 | 0.0002 |
None | 0.0014 | 0.0004 | 0.0008 | 0.001 | 0.002 | 0.0003 | 0.0028 | 0.0007 | 0.0003 | 0.0003 | 0.0003 | 0.0004 | 0.0014 | 0.0091 | 0.0016 | 0.0001 | 0.0068 | 0.0017 |
Nuclear Periphery | 0.0761 | 0.0149 | 0.0243 | 0.0349 | 0.0747 | 0.0347 | 0.0849 | 0.0324 | 0.027 | 0.0254 | 0.0185 | 0.0525 | 0.0213 | 0.017 | 0.016 | 0.0078 | 0.0116 | 0.0157 |
Nucleolus | 0.0003 | 0.0001 | 0.0005 | 0.0009 | 0.0031 | 0.0004 | 0.0003 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 | 0.0002 | 0.0005 | 0.0002 | 0 | 0.0001 | 0.0007 |
Nucleus | 0.0056 | 0.0015 | 0.0047 | 0.0048 | 0.0261 | 0.0038 | 0.0115 | 0.0035 | 0.0031 | 0.0011 | 0.0018 | 0.0103 | 0.0032 | 0.0056 | 0.0017 | 0.0005 | 0.0009 | 0.0034 |
Peroxisomes | 0.0044 | 0 | 0.0035 | 0.0201 | 0.0322 | 0.001 | 0.001 | 0.0006 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0014 | 0 | 0.0001 | 0 | 0.0035 | 0 |
Punctate Nuclear | 0.0014 | 0.0004 | 0.0008 | 0.0006 | 0.0061 | 0.0007 | 0.0024 | 0.0006 | 0.001 | 0.0002 | 0.0002 | 0.0009 | 0.001 | 0.006 | 0.0014 | 0.0001 | 0.0018 | 0.0022 |
Vacuole | 0.0047 | 0.0106 | 0.0097 | 0.0078 | 0.004 | 0.0059 | 0.0056 | 0.0071 | 0.004 | 0.0045 | 0.0029 | 0.0039 | 0.0028 | 0.0026 | 0.0024 | 0.0009 | 0.0013 | 0.0026 |
Vacuole Periphery | 0.0021 | 0.0007 | 0.002 | 0.004 | 0.0041 | 0.0028 | 0.0049 | 0.0019 | 0.0013 | 0.0012 | 0.0024 | 0.0016 | 0.0006 | 0.0005 | 0.0007 | 0.0003 | 0.0005 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 28.5426 | 41.0183 | 15.3385 | 25.4807 | 28.3405 | 16.2494 | 38.7794 | 36.0367 | 25.0236 | 36.8801 |
Translational Efficiency | 0.9202 | 0.6625 | 1.1228 | 0.5427 | 0.6145 | 1.2997 | 0.6741 | 0.5647 | 0.7724 | 0.5922 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
53 | 287 | 248 | 967 | 336 | 112 | 476 | 157 | 389 | 399 | 724 | 1124 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 799.09 | 905.94 | 1380.62 | 1065.22 | 889.39 | 960.93 | 1221.17 | 1138.27 | 877.09 | 921.38 | 1275.79 | 1075.42 |
Standard Deviation | 125.84 | 129.58 | 150.99 | 147.35 | 122.63 | 135.88 | 171.90 | 153.50 | 126.91 | 133.68 | 181.56 | 150.37 |
Intensity Change Log 2 | — | 0.181058 | 0.788886 | 0.414722 | — | 0.111615 | 0.457376 | 0.355955 | — | 0.144896 | 0.623779 | 0.384065 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.008216 | 0.002039 | 0.000579 | 0.003982 | 0.000638 | 0.001024 | 0.006067 | 0.004377 | 0.001671 | 0.001754 | 0.004187 | 0.004038 |
Bud Neck | 0.001038 | 0.001046 | 0.001893 | 0.003723 | 0.000271 | 0.000758 | 0.001338 | 0.004411 | 0.000375 | 0.000965 | 0.001528 | 0.003819 |
Bud Site | 0.007395 | 0.008898 | 0.004223 | 0.017795 | 0.002001 | 0.008640 | 0.005209 | 0.022308 | 0.002736 | 0.008826 | 0.004872 | 0.018425 |
Cell Periphery | 0.005108 | 0.000584 | 0.001526 | 0.002008 | 0.000746 | 0.002297 | 0.000635 | 0.001450 | 0.001341 | 0.001065 | 0.000940 | 0.001930 |
Cytoplasm | 0.065807 | 0.048506 | 0.018805 | 0.051754 | 0.033449 | 0.022619 | 0.052175 | 0.040221 | 0.037857 | 0.041240 | 0.040744 | 0.050143 |
Cytoplasmic Foci | 0.027269 | 0.052026 | 0.001907 | 0.015377 | 0.014500 | 0.017028 | 0.016169 | 0.019741 | 0.016240 | 0.042202 | 0.011284 | 0.015987 |
Eisosomes | 0.002958 | 0.000145 | 0.000019 | 0.000256 | 0.000213 | 0.000129 | 0.000247 | 0.000352 | 0.000587 | 0.000140 | 0.000169 | 0.000269 |
Endoplasmic Reticulum | 0.729959* | 0.726533* | 0.832366* | 0.776429* | 0.878300* | 0.849161* | 0.695306* | 0.731889* | 0.858089* | 0.760955* | 0.742255* | 0.770207* |
Endosome | 0.014721 | 0.024279 | 0.014519 | 0.018200 | 0.006237 | 0.014266 | 0.013809 | 0.020939 | 0.007393 | 0.021468 | 0.014052 | 0.018583 |
Golgi | 0.024449 | 0.023494 | 0.001417 | 0.010399 | 0.011235 | 0.011057 | 0.002618 | 0.006787 | 0.013035 | 0.020003 | 0.002206 | 0.009894 |
Lipid Particles | 0.050170 | 0.028846 | 0.006559 | 0.023550 | 0.017162 | 0.025218 | 0.023353 | 0.021865 | 0.021659 | 0.027828 | 0.017601 | 0.023314 |
Mitochondria | 0.007879 | 0.007481 | 0.000145 | 0.002486 | 0.004492 | 0.005116 | 0.000387 | 0.000412 | 0.004953 | 0.006817 | 0.000304 | 0.002197 |
Mitotic Spindle | 0.001717 | 0.011748 | 0.003363 | 0.003242 | 0.000579 | 0.000856 | 0.002419 | 0.004273 | 0.000734 | 0.008690 | 0.002743 | 0.003386 |
None | 0.001401 | 0.003874 | 0.000070 | 0.000514 | 0.001681 | 0.001505 | 0.001377 | 0.000618 | 0.001643 | 0.003209 | 0.000929 | 0.000528 |
Nuclear Periphery | 0.006815 | 0.004048 | 0.018987 | 0.002289 | 0.002346 | 0.002640 | 0.039315 | 0.003501 | 0.002955 | 0.003653 | 0.032352 | 0.002459 |
Nuclear Periphery Foci | 0.019613 | 0.020592 | 0.070936 | 0.036785 | 0.012208 | 0.012680 | 0.096415 | 0.049050 | 0.013217 | 0.018371 | 0.087687 | 0.038499 |
Nucleolus | 0.000349 | 0.001348 | 0.000013 | 0.000048 | 0.000177 | 0.000088 | 0.000165 | 0.000103 | 0.000200 | 0.000995 | 0.000113 | 0.000056 |
Nucleus | 0.009296 | 0.004734 | 0.001939 | 0.005533 | 0.004052 | 0.001485 | 0.016297 | 0.007181 | 0.004767 | 0.003822 | 0.011379 | 0.005763 |
Peroxisomes | 0.007631 | 0.002607 | 0.000034 | 0.001406 | 0.003020 | 0.001058 | 0.000798 | 0.000506 | 0.003648 | 0.002172 | 0.000536 | 0.001280 |
Vacuole | 0.004361 | 0.019066 | 0.019154 | 0.022078 | 0.004657 | 0.011384 | 0.025433 | 0.059484 | 0.004616 | 0.016909 | 0.023282 | 0.027303 |
Vacuole Periphery | 0.003850 | 0.008107 | 0.001548 | 0.002146 | 0.002037 | 0.010990 | 0.000468 | 0.000534 | 0.002284 | 0.008916 | 0.000838 | 0.001921 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.10 | 1.38 | 0.73 | -1.78 | -4.50 | -0.48 | -2.37 | -1.90 | -1.62 | 0.53 | -0.10 | -1.51 | -2.40 | -2.48 | 0.04 |
Bud Neck | -0.03 | -1.51 | -4.22 | -5.49 | -3.04 | -1.50 | -3.72 | -4.27 | -3.73 | -3.19 | -2.92 | -4.76 | -8.15 | -6.57 | -4.97 |
Bud Site | -0.32 | 0.64 | -2.26 | -3.01 | -4.71 | -1.25 | -2.13 | -2.48 | -1.45 | -2.05 | -2.64 | -1.63 | -6.18 | -3.25 | -5.25 |
Cell Periphery | 1.55 | 1.18 | 1.05 | -4.72 | -0.59 | -1.01 | 0.54 | -1.62 | 0.53 | -1.88 | 0.46 | 0.76 | -1.24 | -1.75 | -2.58 |
Cytoplasm | 1.10 | 2.98 | 0.76 | -0.88 | -6.19 | 1.62 | -2.98 | -1.68 | -2.64 | 0.33 | -0.57 | -0.77 | -2.88 | -2.31 | -2.17 |
Cytoplasmic Foci | -2.34 | 2.92 | 1.31 | 5.69 | -7.69 | -0.44 | -0.68 | -1.12 | -0.64 | -0.73 | -4.74 | 1.30 | -0.11 | 5.15 | -1.94 |
Eisosomes | 1.17 | 1.22 | 1.12 | -2.34 | -5.95 | 0.83 | -0.27 | -0.59 | -1.00 | -0.42 | 1.32 | 1.21 | 0.91 | -2.56 | -1.26 |
Endoplasmic Reticulum | 0.08 | -2.00 | -0.73 | -1.80 | 3.63 | 1.02 | 9.71 | 5.83 | 4.09 | 0.02 | 4.79 | 7.14 | 6.52 | 0.32 | -1.21 |
Endosome | -1.88 | 0.00 | -0.81 | 1.87 | -1.61 | -2.14 | -4.72 | -3.96 | -1.35 | -1.83 | -5.72 | -4.75 | -6.91 | 1.02 | -2.58 |
Golgi | 0.13 | 3.42 | 2.05 | 3.56 | -4.80 | 0.04 | 3.02 | 1.21 | 1.01 | -1.31 | -1.92 | 4.14 | 1.10 | 3.40 | -4.54 |
Lipid Particles | 1.24 | 2.55 | 1.52 | 0.98 | -5.03 | -1.12 | -1.62 | -0.99 | 0.20 | 0.05 | -1.29 | 0.91 | -0.63 | 0.89 | -2.05 |
Mitochondria | 0.09 | 1.84 | 1.26 | 3.01 | -2.66 | -0.29 | 3.53 | 3.49 | 2.63 | -0.19 | -1.15 | 4.03 | 2.02 | 3.40 | -2.50 |
Mitotic Spindle | -2.41 | -0.56 | -1.03 | 2.08 | 0.03 | -0.33 | -1.16 | -1.74 | -1.61 | -1.01 | -2.75 | -1.42 | -3.08 | 1.69 | -0.54 |
None | -1.17 | 1.49 | 0.98 | 1.75 | -2.38 | 0.12 | 0.21 | 0.80 | 0.81 | 0.72 | -0.91 | 0.61 | 1.07 | 1.89 | 0.66 |
Nuclear Periphery | 0.59 | -2.17 | 0.96 | 2.36 | 5.33 | -0.33 | -7.96 | -1.36 | -0.93 | 7.62 | -0.73 | -8.83 | 0.54 | 1.95 | 9.21 |
Nuclear Periphery Foci | -0.14 | -5.16 | -2.53 | -4.17 | 4.11 | -0.10 | -10.62 | -4.23 | -4.02 | 3.97 | -1.68 | -12.36 | -7.47 | -5.84 | 7.64 |
Nucleolus | -1.14 | 1.82 | 1.60 | 1.49 | -4.02 | 1.07 | 0.11 | 0.38 | -0.96 | 0.39 | -1.27 | 1.11 | 1.86 | 1.49 | 1.59 |
Nucleus | 0.61 | 0.94 | 0.35 | -1.32 | -3.40 | 1.18 | -3.71 | -1.84 | -3.56 | 1.99 | 0.40 | -2.56 | -1.05 | -2.76 | 2.34 |
Peroxisomes | 1.93 | 3.22 | 2.60 | 1.55 | -3.07 | 1.85 | 2.08 | 2.31 | 1.58 | 0.95 | 1.19 | 3.22 | 2.30 | 1.49 | -1.81 |
Vacuole | -3.76 | -4.90 | -7.45 | -1.54 | -1.57 | -1.73 | -7.05 | -5.59 | -4.95 | -3.79 | -4.20 | -8.41 | -9.66 | -4.18 | -2.18 |
Vacuole Periphery | -1.45 | 0.89 | 0.68 | 3.26 | -0.48 | -1.36 | 3.61 | 3.57 | 1.59 | -0.33 | -2.94 | 2.52 | 0.49 | 3.06 | -1.46 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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