Standard name
Human Ortholog
Description Protein involved in negative regulation of iron regulon transcription; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.99 1.0 0.98 0.99 0.97 0.93 0.94 0.91 0.91 0.91 0.99 0.98 0.99 0.97 0.97 0.95 0.98 0.97 0.94 0.86 0.91 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.06 0.05 0.05
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 3 2 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 1 0 0 1 1 0 0 2 1 0 0 0 1 0 0 0 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 8 0 2 3 13 10 10 7 9 16 2 2 3 9 16 6 0 0 0 0 0 0
Cytoplasm 277 149 326 316 362 382 585 455 327 402 340 355 366 499 526 210 194 142 272 145 311 149 322 294
Endoplasmic Reticulum 1 0 2 0 3 7 11 19 11 14 12 9 3 4 1 10 3 5 1 0 1 11 16 17
Endosome 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 2 0 0 2 0 0 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 0 0 0 1 0 1
Mitochondria 1 0 0 0 1 1 0 3 10 11 24 13 0 1 0 2 2 0 1 0 0 0 0 1
Nucleus 0 1 1 0 2 1 3 3 2 5 5 4 0 3 1 2 1 1 0 1 1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 4 2 3 12 3 19 9 19 0 1 2 1 2 3 0 1 12 4 7 11
Unique Cell Count 279 150 328 316 368 387 602 490 349 444 374 390 369 507 531 216 201 149 278 150 332 174 355 336
Labelled Cell Count 279 151 338 316 374 397 617 505 366 461 401 416 373 510 534 234 221 160 278 150 332 174 355 336


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.0 16.4 12.8 11.8 12.0 10.3 10.0 10.7 9.6 9.5 9.3 10.2 14.9 15.0 14.9 12.7 12.9 13.5 12.9 13.3 14.1
Std Deviation (1e-4) 2.3 3.2 1.7 1.5 2.1 1.5 1.4 1.5 1.7 1.8 1.7 1.9 2.1 1.9 2.2 3.1 3.6 4.0 1.7 1.7 2.1
Intensity Change (Log2) – – – -0.12 -0.09 -0.3 -0.36 -0.26 -0.41 -0.42 -0.46 -0.33 0.23 0.23 0.22 -0.0 0.01 0.08 0.02 0.06 0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 -1.8 -0.3 -0.4 0.3 -0.9 0 1.2 0 0 0 1.1 3.0 0
Cytoplasm 1.4 -1.3 -0.9 -2.3 -4.4 -4.0 -5.2 -5.1 -5.1 -0.3 -1.3 -0.5 -2.1 -2.5 -3.0
Endoplasmic Reticulum 0 0 0 0 2.9 2.4 2.5 2.5 1.8 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 4.7 3.3 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 3.8 0 4.1 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.3044 11.9681 10.5974 8.8232 9.9689 10.8817 9.0316 13.1316 11.7282 10.4432 11.5947 12.4377 11.7817 14.0414 13.0668 12.1917 9.3289 11.8646
Actin 0.015 0.0003 0.0072 0.0026 0 0.0003 0.0114 0.0001 0.0146 0.0267 0 0.0036 0.0041 0.004 0.0008 0.0159 0.0529 0.0033
Bud 0.0005 0.0002 0.0002 0.0003 0.0001 0.0003 0.0004 0 0.0012 0.0002 0 0.0001 0.0005 0.0004 0.0001 0.0008 0.0012 0.0007
Bud Neck 0.0008 0.0001 0.0006 0.0009 0.0004 0.0007 0.0014 0.0001 0.0008 0.0007 0.0003 0.0004 0.0003 0.0002 0.0001 0.0007 0.0003 0.0006
Bud Periphery 0.0004 0 0.0002 0.0001 0 0.0001 0.0003 0 0.0016 0.0002 0 0.0001 0.0003 0.0002 0 0.0006 0.001 0.0005
Bud Site 0.0009 0.0001 0.0005 0.0003 0 0 0.0016 0 0.0022 0.0004 0 0.0002 0.0028 0.0034 0.0001 0.0038 0.001 0.0018
Cell Periphery 0.0001 0 0 0 0 0 0.0001 0 0.0002 0 0 0 0.0001 0 0 0.0001 0.0001 0
Cytoplasm 0.8493 0.9878 0.9406 0.8475 0.9863 0.9445 0.7404 0.9929 0.9146 0.9333 0.9927 0.9443 0.901 0.9763 0.9658 0.9316 0.7358 0.9109
Cytoplasmic Foci 0.0204 0.0008 0.0062 0.0271 0.0009 0.0016 0.0372 0.0007 0.0073 0.0044 0.0007 0.0155 0.0098 0.0025 0.004 0.0045 0.016 0.0124
Eisosomes 0.0002 0 0.0001 0 0 0 0.0001 0 0.0001 0.0001 0 0 0 0 0 0.0001 0.0001 0
Endoplasmic Reticulum 0.0098 0.0004 0.0017 0.0012 0.0006 0.0017 0.0103 0.0005 0.0044 0.0005 0.0004 0.002 0.0045 0.0004 0.0011 0.0005 0.0076 0.0023
Endosome 0.024 0.0003 0.0043 0.018 0.001 0.0026 0.0496 0.0003 0.0138 0.0058 0.0003 0.0091 0.0341 0.001 0.0055 0.0091 0.0564 0.0211
Golgi 0.0053 0 0.0015 0.0076 0 0.0001 0.0161 0 0.0084 0.017 0 0.0035 0.0062 0.001 0.0025 0.0137 0.0387 0.0074
Lipid Particles 0.0065 0 0.0008 0.059 0 0 0.0103 0 0.0007 0.0017 0 0.003 0.0038 0 0.0002 0.0004 0.0009 0.0134
Mitochondria 0.002 0.0001 0.0083 0.0044 0.0001 0.0008 0.0126 0 0.0082 0.0019 0 0.0014 0.0047 0.0037 0.0068 0.0107 0.0188 0.0103
None 0.0054 0.0007 0.0006 0.0008 0.0004 0.0005 0.0076 0.0005 0.0009 0.0005 0.0004 0.0005 0.0029 0.0004 0.0008 0.0005 0.0016 0.0008
Nuclear Periphery 0.0175 0.0011 0.0023 0.0025 0.0022 0.0053 0.0405 0.001 0.0046 0.0006 0.0007 0.0027 0.0054 0.0006 0.0013 0.0005 0.0411 0.0039
Nucleolus 0.0004 0 0.0001 0.0003 0 0 0.0008 0 0.0001 0 0 0.0001 0.0001 0 0 0 0.0001 0.0001
Nucleus 0.0311 0.0073 0.0198 0.0126 0.0063 0.0378 0.0306 0.0034 0.0109 0.0042 0.0039 0.0089 0.0125 0.0049 0.0101 0.0049 0.0147 0.0054
Peroxisomes 0.0012 0 0.0035 0.0109 0 0 0.0058 0 0.0006 0.0011 0 0.0024 0.0016 0.0005 0.0001 0.0005 0.0028 0.0026
Punctate Nuclear 0.004 0.0002 0.0003 0.0021 0.0001 0.0003 0.0133 0.0001 0.0009 0.0002 0.0001 0.001 0.0013 0.0001 0.0003 0.0001 0.0008 0.0006
Vacuole 0.0036 0.0004 0.001 0.0012 0.001 0.0027 0.0055 0.0002 0.0024 0.0003 0.0002 0.0006 0.0022 0.0002 0.0002 0.0006 0.0047 0.0011
Vacuole Periphery 0.0016 0.0001 0.0003 0.0006 0.0005 0.0005 0.0042 0 0.0016 0.0002 0 0.0005 0.0018 0.0001 0.0001 0.0003 0.0034 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 34.5733 46.4693 38.3859 45.1597 34.6526 49.013 54.1879 60.4318 62.0748 61.3218
Translational Efficiency 1.0012 1.0462 1.0477 1.0097 1.073 0.9189 1.0044 0.9392 0.9158 0.8173

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1751 108 388 140 184 961 149 128 1935 1069 537 268

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1171.93 1495.61 1835.43 1741.79 1454.88 1413.12 1726.82 1713.45 1198.84 1421.45 1805.29 1728.25
Standard Deviation 180.71 264.10 252.16 322.51 222.47 212.85 243.27 273.88 202.84 219.98 254.42 300.60
Intensity Change Log 2 — 0.351848 0.647232 0.571684 — -0.042016 0.247218 0.236004 — 0.147078 0.439477 0.395474

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000106 0.000267 0.000519 0.010020 0.000500 0.000126 0.000509 0.000674 0.000144 0.000140 0.000516 0.005556
Bud Neck 0.000290 0.000950 0.002861 0.017800 0.001189 0.000507 0.002076 0.003866 0.000376 0.000552 0.002643 0.011145
Bud Site 0.004384 0.009668 0.007420 0.089462 0.010675 0.003634 0.006556 0.015728 0.004983 0.004244 0.007180 0.054246
Cell Periphery 0.000048 0.000171 0.000590 0.000794 0.000188 0.000041 0.000297 0.000423 0.000062 0.000054 0.000509 0.000617
Cytoplasm 0.918836* 0.814023* 0.809008* 0.459347* 0.826483* 0.871668* 0.733848* 0.731392* 0.910054* 0.865844* 0.788153* 0.589279*
Cytoplasmic Foci 0.031379 0.068421 0.023426 0.113349 0.058393 0.038002 0.026119 0.046593 0.033948 0.041075 0.024173 0.081466
Eisosomes 0.000002 0.000007 0.000003 0.000047 0.000008 0.000003 0.000007 0.000012 0.000003 0.000004 0.000004 0.000030
Endoplasmic Reticulum 0.000566 0.002069 0.000311 0.002808 0.001376 0.001059 0.000371 0.001163 0.000643 0.001161 0.000327 0.002022
Endosome 0.001231 0.005258 0.001571 0.023227 0.003573 0.003602 0.001398 0.010323 0.001454 0.003770 0.001523 0.017064
Golgi 0.001647 0.011505 0.002456 0.031724 0.001748 0.001826 0.000468 0.001722 0.001656 0.002804 0.001904 0.017395
Lipid Particles 0.000323 0.001583 0.003819 0.013148 0.001960 0.000773 0.003304 0.006777 0.000479 0.000855 0.003676 0.010105
Mitochondria 0.000304 0.005753 0.000094 0.009961 0.000072 0.000117 0.000086 0.006924 0.000282 0.000686 0.000092 0.008510
Mitotic Spindle 0.000785 0.013499 0.012119 0.070832 0.006039 0.001296 0.008605 0.003013 0.001285 0.002529 0.011144 0.038441
None 0.003687 0.004315 0.006199 0.001479 0.000998 0.002443 0.008603 0.000931 0.003431 0.002632 0.006866 0.001217
Nuclear Periphery 0.000024 0.000120 0.000271 0.000769 0.000174 0.000141 0.000400 0.000570 0.000038 0.000139 0.000307 0.000674
Nuclear Periphery Foci 0.001751 0.006724 0.006675 0.012351 0.014102 0.003317 0.005921 0.005468 0.002926 0.003661 0.006466 0.009064
Nucleolus 0.000093 0.000087 0.000104 0.000232 0.000076 0.000194 0.000105 0.000253 0.000092 0.000183 0.000104 0.000242
Nucleus 0.017809 0.030142 0.095835 0.023030 0.030199 0.030637 0.133298* 0.024420 0.018987 0.030587 0.106230 0.023694
Peroxisomes 0.000127 0.000402 0.000059 0.006923 0.000185 0.000170 0.000114 0.001207 0.000133 0.000193 0.000074 0.004193
Vacuole 0.015003 0.022827 0.026238 0.106087 0.041444 0.039561 0.067816 0.116787 0.017517 0.037870 0.037774 0.111198
Vacuole Periphery 0.001603 0.002214 0.000425 0.006610 0.000620 0.000883 0.000100 0.021754 0.001509 0.001017 0.000334 0.013843

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.40 -7.88 -1.73 -1.63 -1.40 3.72 -1.31 0.68 -3.14 1.99 -0.59 -8.69 -1.83 -1.82 -1.25
Bud Neck -4.31 -9.23 -7.27 -6.76 -4.78 4.88 -4.63 -5.11 -6.50 -0.77 -4.45 -10.82 -8.46 -8.26 -4.40
Bud Site -2.17 -4.91 -6.16 -5.25 -5.55 4.29 0.81 -2.08 -4.67 -2.71 0.70 -5.10 -6.86 -6.97 -5.93
Cell Periphery -4.25 -10.46 -7.81 -5.90 0.56 5.23 -3.67 -1.78 -5.74 1.79 1.01 -11.63 -8.87 -8.97 2.21
Cytoplasm 5.39 15.86 15.22 8.65 8.69 -3.59 8.76 5.97 9.07 -1.44 8.34 20.03 17.91 14.80 6.80
Cytoplasmic Foci -3.50 0.15 -5.80 -1.30 -5.79 3.31 4.08 0.81 -1.96 -2.71 -2.54 1.22 -5.88 -4.51 -6.28
Eisosomes -3.27 -5.28 -4.34 -3.23 -3.69 4.32 -2.12 -0.41 -3.34 1.41 -2.82 -7.02 -5.09 -4.65 -3.40
Endoplasmic Reticulum -0.89 1.19 -0.62 0.57 -0.83 0.91 2.78 2.78 1.64 0.60 -1.28 1.96 -0.05 0.90 -0.62
Endosome -2.47 -3.35 -3.82 -1.72 -3.34 0.29 2.70 -0.55 -0.74 -2.06 -4.54 -2.73 -4.39 -2.54 -3.84
Golgi -1.46 -2.38 -1.99 -0.10 -1.77 -0.12 3.20 2.05 1.84 -1.42 -1.19 -1.10 -1.79 -1.37 -1.64
Lipid Particles -3.08 -9.21 -4.67 -4.23 -3.05 3.31 -4.25 -3.38 -4.06 -1.31 -3.14 -10.90 -5.98 -5.78 -3.29
Mitochondria -1.08 1.30 -1.28 0.39 -1.37 -1.00 -1.17 -1.36 -1.32 -1.32 -0.70 1.27 -1.69 -1.38 -1.83
Mitotic Spindle -1.99 -4.14 -3.52 -2.08 -2.45 1.04 -1.65 0.62 -2.13 2.46 -1.57 -4.80 -3.54 -3.31 -1.45
None -0.33 -0.91 4.20 2.81 2.63 -4.67 -3.92 0.99 5.96 4.13 2.04 -1.47 5.43 4.73 3.83
Nuclear Periphery -3.48 -11.34 -4.81 -3.36 -0.91 0.79 -6.83 -1.48 -1.97 4.23 -2.28 -14.27 -5.82 -3.71 1.26
Nuclear Periphery Foci -4.75 -9.44 -4.61 -1.37 -1.07 4.45 2.92 2.25 -2.28 -0.32 -2.05 -8.35 -4.66 -3.87 -0.81
Nucleolus 0.10 -4.25 -3.54 -3.35 -2.03 -3.72 -4.67 -2.37 0.84 -0.15 -3.43 -5.70 -3.81 0.51 -1.62
Nucleus -3.43 -16.56 -3.03 0.50 11.42 0.11 -9.77 2.52 3.28 11.15 -8.74 -20.03 -2.63 2.05 16.27
Peroxisomes -1.86 2.35 -4.17 -3.82 -4.22 0.74 1.20 -2.80 -2.97 -3.06 -2.18 1.69 -4.57 -4.43 -4.62
Vacuole -2.91 -6.74 -10.09 -8.75 -7.78 0.76 -7.58 -8.24 -8.91 -1.13 -8.55 -10.63 -14.35 -12.20 -8.06
Vacuole Periphery -0.23 3.64 -0.79 -0.29 -2.44 -1.02 2.57 -1.32 -1.25 -1.44 1.64 3.99 -1.24 -1.56 -1.89
Standard name
Human Ortholog
Description Protein involved in negative regulation of iron regulon transcription; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Fra1

Fra1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fra1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available