Standard name | |
---|---|
Human Ortholog | |
Description | Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; during oxidative stress exports spermine, spermidine from the cell, which controls timing of expression of stress-responsive genes; phosphorylation enhances activity and sorting to the plasma membrane |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 30 | 37 | 80 | 43 | 31 | 58 | 62 | 93 | 46 | 78 | 52 | 78 | 96 | 99 | 53 | 42 | 21 | 28 | 5 | 8 | 11 | 12 | 8 | 16 |
Bud | 4 | 6 | 4 | 2 | 1 | 10 | 1 | 16 | 4 | 9 | 3 | 3 | 3 | 3 | 4 | 12 | 6 | 4 | 4 | 4 | 1 | 2 | 4 | 14 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 1 | 3 | 3 | 7 |
Bud Site | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 88 | 104 | 177 | 103 | 99 | 83 | 109 | 136 | 68 | 117 | 80 | 125 | 466 | 466 | 454 | 181 | 64 | 143 | 111 | 120 | 213 | 211 | 214 | 270 |
Cytoplasm | 11 | 16 | 16 | 12 | 14 | 40 | 25 | 34 | 20 | 25 | 18 | 20 | 40 | 40 | 25 | 20 | 10 | 11 | 4 | 1 | 0 | 2 | 3 | 1 |
Endoplasmic Reticulum | 11 | 10 | 19 | 52 | 59 | 67 | 126 | 140 | 46 | 90 | 51 | 61 | 16 | 21 | 25 | 4 | 3 | 9 | 8 | 7 | 16 | 10 | 4 | 9 |
Endosome | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 3 | 2 | 1 | 1 | 4 | 1 | 2 | 0 | 4 | 0 | 0 | 3 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 4 | 8 | 40 | 20 | 3 | 50 | 31 | 34 | 39 | 53 | 46 | 42 | 1 | 1 | 0 | 8 | 7 | 21 | 1 | 1 | 3 | 9 | 7 | 7 |
Nucleus | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 1 | 1 | 1 | 1 | 2 | 2 | 1 | 0 | 0 | 4 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 2 | 7 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 3 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 3 | 2 | 2 |
Unique Cell Count | 115 | 129 | 226 | 157 | 155 | 210 | 249 | 312 | 155 | 241 | 160 | 209 | 512 | 517 | 511 | 215 | 83 | 165 | 139 | 151 | 249 | 258 | 254 | 334 |
Labelled Cell Count | 152 | 184 | 348 | 236 | 212 | 314 | 359 | 460 | 226 | 374 | 251 | 337 | 625 | 634 | 569 | 267 | 111 | 221 | 139 | 151 | 249 | 258 | 254 | 334 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 21.0 | 15.2 | 16.6 | 20.3 | 20.1 | 17.5 | 20.3 | 20.8 | 20.8 | 23.2 | 22.8 | 25.4 | 24.0 | 24.0 | 26.1 | 16.7 | 21.1 | 24.6 | 18.3 | 16.1 | 16.3 |
Std Deviation (1e-4) | 7.3 | 6.8 | 6.0 | 6.4 | 6.0 | 5.9 | 5.2 | 5.6 | 6.9 | 5.7 | 6.4 | 6.9 | 6.3 | 6.3 | 6.8 | 4.7 | 6.4 | 7.7 | 7.6 | 5.5 | 5.7 |
Intensity Change (Log2) | – | – | – | 0.28 | 0.27 | 0.07 | 0.28 | 0.32 | 0.32 | 0.48 | 0.46 | 0.61 | 0.53 | 0.53 | 0.65 | 0.01 | 0.34 | 0.56 | 0.14 | -0.04 | -0.03 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.7 | -3.2 | -1.7 | -2.5 | -1.4 | -1.2 | -0.7 | -0.6 | 0.4 | -4.9 | -4.8 | -8.1 | -3.7 | -1.7 | -4.0 |
Bud | 0 | 0 | 1.8 | 0 | 2.0 | 0 | 1.3 | 0 | 0 | 0 | 0 | 0 | 2.1 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -2.8 | -3.1 | -8.2 | -7.7 | -8.1 | -6.9 | -6.7 | -5.8 | -4.2 | 4.7 | 4.3 | 3.8 | 1.6 | -0.2 | 2.1 |
Cytoplasm | 0.2 | 0.7 | 3.7 | 1.1 | 1.5 | 1.9 | 1.3 | 1.4 | 0.9 | 0.3 | 0.3 | -1.2 | 0.9 | 1.4 | -0.2 |
Endoplasmic Reticulum | 6.1 | 7.0 | 6.2 | 10.0 | 9.1 | 5.4 | 7.4 | 5.9 | 5.6 | -3.1 | -2.4 | -1.9 | -3.1 | -1.5 | -1.1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -1.3 | -4.8 | 1.6 | -1.6 | -2.3 | 1.8 | 1.2 | 2.6 | 0.6 | -9.6 | -9.6 | -9.8 | -4.7 | -2.0 | -1.3 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 26.6095 | 41.0922 | 37.3847 | 32.5871 | 30.9688 | 37.0333 | 25.3349 | 35.7789 | 31.3581 | 28.2075 | 31.7626 | 33.4894 | 23.0471 | 33.2447 | 27.7067 | 25.8191 | 19.2526 | 28.289 |
Actin | 0.003 | 0.0004 | 0.0037 | 0.0068 | 0.0041 | 0.0007 | 0.0062 | 0.0002 | 0.0098 | 0.0006 | 0.0209 | 0.009 | 0.0304 | 0 | 0.0122 | 0.0015 | 0.0729 | 0.0001 |
Bud | 0.0011 | 0.0001 | 0.0007 | 0.0008 | 0.0003 | 0.0001 | 0.0017 | 0.0001 | 0.0012 | 0.0049 | 0.0001 | 0.0005 | 0.0033 | 0.0001 | 0.0002 | 0.0001 | 0.0007 | 0.0001 |
Bud Neck | 0.0003 | 0.0003 | 0.0009 | 0.0073 | 0.0035 | 0.0005 | 0.0006 | 0.0003 | 0.0018 | 0.0025 | 0.0011 | 0.0009 | 0.0018 | 0.0005 | 0.002 | 0.0009 | 0.0008 | 0.0017 |
Bud Periphery | 0.0146 | 0.0004 | 0.0047 | 0.0037 | 0.0015 | 0.0015 | 0.0116 | 0.0009 | 0.0094 | 0.0128 | 0.0019 | 0.0079 | 0.0166 | 0.0006 | 0.0018 | 0.003 | 0.0072 | 0.0024 |
Bud Site | 0.0018 | 0.0076 | 0.0022 | 0.002 | 0.0012 | 0.0001 | 0.0048 | 0.0026 | 0.0217 | 0.0098 | 0.0005 | 0.0003 | 0.0111 | 0.0139 | 0.0026 | 0.0005 | 0.0037 | 0.0001 |
Cell Periphery | 0.4536 | 0.5053 | 0.5182 | 0.3275 | 0.3832 | 0.3868 | 0.4195 | 0.5222 | 0.5173 | 0.451 | 0.4614 | 0.3788 | 0.3843 | 0.4488 | 0.5828 | 0.4752 | 0.3373 | 0.4055 |
Cytoplasm | 0.0259 | 0.0363 | 0.0129 | 0.0161 | 0.0279 | 0.0067 | 0.0032 | 0.0142 | 0.0078 | 0.0078 | 0.0082 | 0.005 | 0.0078 | 0.0127 | 0.0236 | 0.0075 | 0.0114 | 0.0035 |
Cytoplasmic Foci | 0.0055 | 0.0001 | 0.0009 | 0.0052 | 0.006 | 0.0017 | 0.0007 | 0.0001 | 0.0025 | 0.0012 | 0.0063 | 0.0014 | 0.0125 | 0 | 0.0041 | 0 | 0.0117 | 0 |
Eisosomes | 0.0166 | 0.0028 | 0.0045 | 0.0006 | 0.0008 | 0.0014 | 0.004 | 0.0015 | 0.0038 | 0.003 | 0.0009 | 0.001 | 0.0114 | 0.0008 | 0.0091 | 0.0027 | 0.0074 | 0.0013 |
Endoplasmic Reticulum | 0.3628 | 0.4005 | 0.3743 | 0.5318 | 0.4397 | 0.5628 | 0.3757 | 0.4145 | 0.3637 | 0.4401 | 0.4253 | 0.5576 | 0.4192 | 0.4861 | 0.3035 | 0.424 | 0.398 | 0.5669 |
Endosome | 0.0045 | 0 | 0.0019 | 0.0077 | 0.0145 | 0.0023 | 0.0033 | 0.0001 | 0.0008 | 0.0003 | 0.018 | 0.0018 | 0.0013 | 0 | 0.0005 | 0.0001 | 0.0486 | 0 |
Golgi | 0.0011 | 0.0001 | 0.0009 | 0.0014 | 0.0025 | 0.0005 | 0.0011 | 0.0002 | 0.001 | 0.0004 | 0.0077 | 0.0013 | 0.0043 | 0.0001 | 0.0016 | 0.0002 | 0.0265 | 0.0002 |
Lipid Particles | 0.0042 | 0.0053 | 0.004 | 0.0056 | 0.0138 | 0.0029 | 0.0037 | 0.0057 | 0.003 | 0.0031 | 0.0122 | 0.0025 | 0.022 | 0.0015 | 0.0068 | 0.0011 | 0.0323 | 0.0016 |
Mitochondria | 0.0109 | 0 | 0.0047 | 0.0068 | 0.0019 | 0.0005 | 0.0643 | 0 | 0.0024 | 0.0041 | 0.0013 | 0.0089 | 0.0012 | 0 | 0.0041 | 0.0589 | 0.0178 | 0 |
None | 0.0072 | 0.0001 | 0.0041 | 0.0092 | 0.0051 | 0.0005 | 0.0019 | 0.0001 | 0.0073 | 0.0021 | 0.0039 | 0.0004 | 0.0007 | 0 | 0.0002 | 0.0001 | 0.0029 | 0.0001 |
Nuclear Periphery | 0.0339 | 0.0231 | 0.0363 | 0.0304 | 0.0151 | 0.0125 | 0.0488 | 0.023 | 0.0253 | 0.0197 | 0.0164 | 0.0111 | 0.0295 | 0.0239 | 0.0236 | 0.0129 | 0.0091 | 0.0095 |
Nucleolus | 0.0031 | 0 | 0.0014 | 0.0032 | 0.0027 | 0.0001 | 0.0021 | 0 | 0.0004 | 0.0104 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0004 | 0 |
Nucleus | 0.0105 | 0.0002 | 0.0013 | 0.01 | 0.0057 | 0.0004 | 0.0149 | 0.0002 | 0.0006 | 0.002 | 0.0004 | 0.0002 | 0.0005 | 0.0002 | 0.0003 | 0.0001 | 0.0008 | 0.0001 |
Peroxisomes | 0.0002 | 0 | 0.0003 | 0.0008 | 0.0065 | 0.0002 | 0.0006 | 0 | 0.0014 | 0.0007 | 0.0033 | 0.0009 | 0.0243 | 0 | 0.0089 | 0 | 0.0025 | 0 |
Punctate Nuclear | 0.0037 | 0 | 0.0011 | 0.0077 | 0.0385 | 0.0047 | 0.0044 | 0 | 0.0003 | 0.0001 | 0.0023 | 0.0016 | 0.0005 | 0 | 0.0001 | 0 | 0.001 | 0 |
Vacuole | 0.0327 | 0.017 | 0.0196 | 0.0131 | 0.0247 | 0.0128 | 0.0236 | 0.0138 | 0.0181 | 0.0211 | 0.0073 | 0.0086 | 0.0167 | 0.0105 | 0.0116 | 0.0108 | 0.0056 | 0.0069 |
Vacuole Periphery | 0.0025 | 0.0001 | 0.0015 | 0.0024 | 0.0007 | 0.0003 | 0.0032 | 0.0001 | 0.0006 | 0.0024 | 0.0005 | 0.0003 | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0013 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.0253 | 15.6834 | 25.3538 | 28.2589 | 16.6539 | 11.2722 | 12.3179 | 12.4228 | 17.5583 | 11.3927 |
Translational Efficiency | 0.9387 | 1.2787 | 1.2822 | 1.2024 | 0.7625 | 1.0605 | 0.8599 | 1.1064 | 1.0391 | 0.6184 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
99 | 1559 | 136 | 722 | 1380 | 1810 | 2165 | 1710 | 1479 | 3369 | 2301 | 2432 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 2263.60 | 2422.00 | 2012.88 | 2467.97 | 1197.13 | 2278.28 | 2881.26 | 2425.29 | 1268.52 | 2344.79 | 2829.93 | 2437.96 |
Standard Deviation | 488.06 | 602.98 | 509.05 | 636.80 | 274.38 | 505.37 | 599.95 | 588.40 | 396.51 | 557.31 | 629.22 | 603.49 |
Intensity Change Log 2 | — | 0.097580 | -0.169358 | 0.124706 | — | 0.928365 | 1.267120 | 1.018577 | — | 0.441670 | 0.499979 | 0.499720 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001045 | 0.001260 | 0.005873 | 0.001473 | 0.001989 | 0.001148 | 0.001624 | 0.000866 | 0.001925 | 0.001200 | 0.001875 | 0.001046 |
Bud Neck | 0.002291 | 0.001470 | 0.013975 | 0.001747 | 0.003040 | 0.001460 | 0.001374 | 0.000402 | 0.002990 | 0.001464 | 0.002118 | 0.000801 |
Bud Site | 0.010306 | 0.015175 | 0.066248 | 0.014631 | 0.026178 | 0.011974 | 0.008215 | 0.005173 | 0.025116 | 0.013455 | 0.011645 | 0.007981 |
Cell Periphery | 0.883037* | 0.937227* | 0.868458* | 0.952961* | 0.804439* | 0.869441* | 0.894600* | 0.981669* | 0.809700* | 0.900809* | 0.893055* | 0.973147* |
Cytoplasm | 0.006338 | 0.002034 | 0.000648 | 0.003970 | 0.014219 | 0.008471 | 0.008976 | 0.000392 | 0.013691 | 0.005492 | 0.008484 | 0.001454 |
Cytoplasmic Foci | 0.003509 | 0.001064 | 0.000200 | 0.000253 | 0.002685 | 0.000730 | 0.000329 | 0.000232 | 0.002740 | 0.000885 | 0.000321 | 0.000238 |
Eisosomes | 0.001049 | 0.002571 | 0.026128 | 0.003300 | 0.001789 | 0.001790 | 0.002644 | 0.001941 | 0.001740 | 0.002151 | 0.004032 | 0.002344 |
Endoplasmic Reticulum | 0.009807 | 0.010207 | 0.005211 | 0.001634 | 0.114134* | 0.038969 | 0.011286 | 0.001277 | 0.107151* | 0.025659 | 0.010927 | 0.001383 |
Endosome | 0.008099 | 0.001360 | 0.000699 | 0.000475 | 0.002950 | 0.001830 | 0.000898 | 0.000409 | 0.003294 | 0.001613 | 0.000886 | 0.000428 |
Golgi | 0.015180 | 0.002503 | 0.001076 | 0.000754 | 0.002371 | 0.004772 | 0.002091 | 0.000470 | 0.003228 | 0.003722 | 0.002031 | 0.000554 |
Lipid Particles | 0.001380 | 0.001386 | 0.000098 | 0.001874 | 0.001308 | 0.004968 | 0.011402 | 0.000089 | 0.001313 | 0.003311 | 0.010734 | 0.000619 |
Mitochondria | 0.002635 | 0.000987 | 0.001151 | 0.001368 | 0.001721 | 0.000739 | 0.000586 | 0.000588 | 0.001782 | 0.000854 | 0.000620 | 0.000819 |
Mitotic Spindle | 0.005117 | 0.000944 | 0.001349 | 0.001345 | 0.001216 | 0.001943 | 0.001428 | 0.000317 | 0.001477 | 0.001481 | 0.001424 | 0.000623 |
None | 0.000058 | 0.000083 | 0.000065 | 0.000031 | 0.000089 | 0.000072 | 0.000078 | 0.000014 | 0.000087 | 0.000077 | 0.000077 | 0.000019 |
Nuclear Periphery | 0.001758 | 0.003681 | 0.000501 | 0.000543 | 0.005343 | 0.014709 | 0.010207 | 0.001021 | 0.005103 | 0.009606 | 0.009634 | 0.000879 |
Nuclear Periphery Foci | 0.001116 | 0.000482 | 0.000174 | 0.000258 | 0.000766 | 0.000925 | 0.000683 | 0.000061 | 0.000789 | 0.000720 | 0.000653 | 0.000120 |
Nucleolus | 0.000048 | 0.000112 | 0.000061 | 0.000052 | 0.000064 | 0.000112 | 0.000105 | 0.000042 | 0.000063 | 0.000112 | 0.000102 | 0.000045 |
Nucleus | 0.000344 | 0.000218 | 0.000509 | 0.000190 | 0.000619 | 0.000325 | 0.000752 | 0.000061 | 0.000601 | 0.000276 | 0.000738 | 0.000099 |
Peroxisomes | 0.000282 | 0.000136 | 0.000010 | 0.000034 | 0.005029 | 0.000982 | 0.000473 | 0.000016 | 0.004711 | 0.000590 | 0.000446 | 0.000021 |
Vacuole | 0.030600 | 0.013118 | 0.006652 | 0.011369 | 0.007942 | 0.029323 | 0.038353 | 0.003622 | 0.009458 | 0.021824 | 0.036479 | 0.005922 |
Vacuole Periphery | 0.016003 | 0.003980 | 0.000913 | 0.001739 | 0.002109 | 0.005319 | 0.003896 | 0.001339 | 0.003039 | 0.004699 | 0.003719 | 0.001458 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.47 | -3.00 | -0.55 | -0.28 | 2.67 | 2.71 | 0.97 | 3.32 | 1.00 | 2.41 | 2.64 | 0.04 | 2.64 | 0.65 | 2.55 |
Bud Neck | 0.92 | -6.67 | 0.63 | -0.95 | 8.24 | 4.78 | 5.82 | 9.91 | 4.95 | 7.71 | 5.30 | 2.95 | 8.36 | 4.10 | 7.81 |
Bud Site | -1.18 | -4.82 | -0.97 | 0.22 | 4.58 | 4.64 | 6.41 | 7.52 | 3.65 | 2.20 | 4.18 | 4.94 | 6.49 | 3.41 | 2.42 |
Cell Periphery | -2.20 | 0.48 | -2.84 | -2.81 | -5.90 | -8.11 | -12.08 | -25.16 | -24.69 | -25.35* | -12.66 | -11.54 | -23.91 | -21.33 | -23.42 |
Cytoplasm | 1.68 | 2.33 | 0.84 | -1.25 | -2.41 | 2.86 | 2.86 | 8.95 | 6.15 | 8.55 | 5.04 | 3.02 | 8.13 | 4.78 | 6.86 |
Cytoplasmic Foci | 1.18 | 1.60 | 1.57 | 1.87 | -0.47 | 3.07 | 3.73 | 3.74 | 2.51 | 0.53 | 2.92 | 4.01 | 4.05 | 2.72 | 0.62 |
Eisosomes | -6.14 | -4.97 | -5.49 | -1.63 | 4.51 | -0.01 | -2.94 | -0.56 | -0.55 | 2.61 | -1.70 | -5.46 | -2.37 | -0.85 | 4.12 |
Endoplasmic Reticulum | -0.09 | 0.88 | 1.59 | 7.13 | 3.25 | 12.94 | 19.14 | 21.24 | 15.95 | 12.33 | 15.67 | 18.97 | 21.08 | 17.32 | 12.45 |
Endosome | 1.87 | 2.06 | 2.11 | 3.23 | 1.21 | 7.04 | 14.21 | 11.60 | 7.70 | 2.86 | 5.61 | 8.58 | 9.35 | 7.04 | 3.47 |
Golgi | 1.80 | 2.01 | 2.06 | 3.18 | 1.35 | -4.40 | 0.64 | 4.19 | 11.25 | 8.25 | -0.72 | 1.91 | 4.24 | 9.57 | 9.12 |
Lipid Particles | -0.02 | 2.77 | -0.66 | -0.62 | -2.95 | -4.52 | -9.02 | 4.95 | 5.87 | 9.99 | -3.93 | -8.93 | 2.33 | 4.90 | 9.41 |
Mitochondria | 0.96 | 0.87 | 0.67 | -0.41 | -0.24 | 3.94 | 5.06 | 4.15 | 0.77 | 0.03 | 3.62 | 4.85 | 2.60 | 0.14 | -0.66 |
Mitotic Spindle | 1.42 | 1.29 | 1.20 | -0.51 | -0.10 | -8.34 | -3.01 | 15.00* | 24.20* | 25.66* | 0.03 | 0.30 | 2.32 | 2.82 | 2.62 |
None | -0.71 | -0.28 | 1.08 | 1.82 | 4.51 | 0.84 | 0.55 | 4.08 | 6.79 | 10.28 | 0.39 | 0.51 | 3.93 | 4.08 | 9.80 |
Nuclear Periphery | -4.55 | 3.96 | 3.87 | 10.76 | -0.50 | -12.30 | -8.27 | 10.54 | 19.83 | 18.67 | -8.60 | -8.17 | 11.48 | 21.10 | 19.38 |
Nuclear Periphery Foci | 1.40 | 2.20 | 1.99 | 1.41 | -1.81 | -2.54 | 1.58 | 11.41 | 12.26 | 10.63 | 0.78 | 2.42 | 11.30 | 6.94 | 11.08 |
Nucleolus | -4.31 | -0.92 | -0.43 | 5.07 | 0.80 | -6.42 | -6.14 | 3.65 | 15.03 | 19.32* | -6.10 | -6.26 | 3.14 | 11.30 | 18.18 |
Nucleus | 0.91 | -1.11 | 1.19 | 0.92 | 4.49 | 1.29 | -0.58 | 2.44 | 10.84 | 12.66 | 1.51 | -0.64 | 2.35 | 8.61 | 12.36 |
Peroxisomes | 0.76 | 1.37 | 1.25 | 3.37 | -2.96 | 5.97 | 6.88 | 7.60 | 6.20 | 7.70 | 6.62 | 6.89 | 7.61 | 6.68 | 7.59 |
Vacuole | 3.36 | 4.61 | 3.64 | 1.22 | -3.38 | -17.13 | -23.01 | 6.21 | 21.52 | 27.35 | -12.93 | -20.67 | 4.36 | 18.53 | 24.68 |
Vacuole Periphery | 1.40 | 1.75 | 1.66 | 8.89 | -4.55 | -14.16 | -9.58 | 4.35 | 22.40 | 20.92* | -2.70 | -1.11 | 2.62 | 21.93 | 19.99* |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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