Standard name | |
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Human Ortholog | |
Description | Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; forms a complex with Kel1p and Kel2p that regulates Bnr1p (formin) to affect actin cable assembly, cytokinesis, and polarized growth; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
Bud | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 2 | 6 | 6 | 3 | 4 | 0 | 2 | 0 | 0 | 0 | 0 | 9 | 9 | 6 | 1 | 19 | 23 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 3 | 8 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 206 | 156 | 192 | 100 | 109 | 154 | 292 | 301 | 211 | 237 | 188 | 266 | 119 | 104 | 137 | 199 | 139 | 144 | 177 | 150 | 210 | 84 | 68 | 59 |
Endoplasmic Reticulum | 3 | 5 | 8 | 3 | 3 | 5 | 5 | 8 | 2 | 0 | 1 | 5 | 5 | 3 | 2 | 14 | 4 | 9 | 1 | 0 | 1 | 8 | 25 | 22 |
Endosome | 1 | 1 | 0 | 1 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 4 | 0 | 2 | 0 | 7 | 2 | 7 | 1 | 16 | 21 |
Golgi | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 2 | 3 | 0 | 0 | 2 | 0 | 0 | 3 |
Mitochondria | 2 | 4 | 0 | 1 | 4 | 11 | 30 | 17 | 64 | 78 | 89 | 49 | 3 | 1 | 2 | 2 | 3 | 1 | 2 | 2 | 4 | 1 | 4 | 7 |
Nucleus | 16 | 10 | 27 | 43 | 81 | 33 | 34 | 40 | 15 | 16 | 7 | 22 | 19 | 54 | 32 | 37 | 24 | 43 | 101 | 88 | 107 | 17 | 87 | 83 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 6 | 0 | 1 | 1 | 5 | 5 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 5 | 14 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 2 | 2 | 1 | 7 | 9 |
Vac/Vac Membrane | 92 | 61 | 138 | 136 | 151 | 72 | 79 | 76 | 48 | 52 | 50 | 52 | 182 | 235 | 219 | 266 | 149 | 168 | 18 | 23 | 51 | 27 | 103 | 101 |
Unique Cell Count | 282 | 220 | 324 | 245 | 308 | 249 | 406 | 412 | 298 | 343 | 298 | 373 | 303 | 361 | 371 | 422 | 265 | 290 | 339 | 286 | 399 | 152 | 360 | 376 |
Labelled Cell Count | 320 | 239 | 370 | 284 | 352 | 277 | 445 | 446 | 346 | 390 | 339 | 402 | 332 | 403 | 397 | 530 | 325 | 368 | 339 | 286 | 399 | 152 | 360 | 376 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.3 | 8.9 | 9.9 | 9.2 | 8.7 | 6.7 | 6.5 | 6.6 | 5.5 | 5.2 | 4.9 | 5.4 | 10.1 | 9.9 | 9.6 | 13.4 | 14.3 | 13.8 | 10.0 | 9.8 | 10.4 |
Std Deviation (1e-4) | 1.8 | 2.1 | 1.3 | 1.4 | 1.3 | 1.1 | 1.1 | 1.1 | 1.1 | 1.0 | 1.0 | 0.9 | 1.6 | 1.7 | 1.7 | 2.7 | 2.7 | 2.4 | 1.6 | 1.8 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.19 | -0.56 | -0.61 | -0.6 | -0.85 | -0.92 | -1.03 | -0.87 | 0.02 | -0.01 | -0.05 | 0.43 | 0.52 | 0.47 | 0.01 | -0.01 | 0.07 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -4.4 | -6.0 | 0.6 | 3.6 | 4.0 | 3.0 | 2.7 | 1.0 | 3.3 | -5.0 | -8.0 | -5.9 | -3.3 | -1.7 | -2.4 |
Endoplasmic Reticulum | 0 | -1.4 | -0.4 | -1.3 | -0.5 | 0 | 0 | 0 | -1.1 | -0.7 | -1.7 | 0 | 0.7 | -0.8 | 0.5 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 5.0 | 3.7 | 8.8 | 9.1 | 0 | 6.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 3.3 | 6.0 | 1.9 | 0 | 0.6 | -1.6 | -1.9 | -3.3 | -1.3 | -1.0 | 2.7 | 0.1 | 0.2 | 0.3 | 2.5 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 3.1 | 1.6 | -3.4 | -6.8 | -7.2 | -7.2 | -7.8 | -7.0 | -8.5 | 4.4 | 5.9 | 4.3 | 5.6 | 3.3 | 3.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.8622 | 6.3968 | 5.5645 | 5.0263 | 4.5186 | 5.5152 | 5.4091 | 7.3396 | 6.5158 | 6.1828 | 4.9668 | 6.2215 | 4.8617 | 7.7004 | 6.1386 | 6.1478 | 5.6101 | 6.1079 |
Actin | 0.0028 | 0.0001 | 0.0012 | 0.0003 | 0.0001 | 0.0001 | 0.0126 | 0.0003 | 0.0036 | 0.0006 | 0.0007 | 0.0002 | 0.0133 | 0.0002 | 0.0012 | 0.0245 | 0.0031 | 0.0036 |
Bud | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0009 | 0.0002 | 0.0004 | 0.0002 | 0.0003 | 0.0001 | 0.0006 | 0.0002 | 0.0003 | 0.0005 | 0.0002 | 0.0002 |
Bud Neck | 0.0042 | 0.0005 | 0.0008 | 0.0012 | 0.001 | 0.0017 | 0.0036 | 0.0005 | 0.0008 | 0.0008 | 0.0011 | 0.0016 | 0.0029 | 0.0004 | 0.0013 | 0.0013 | 0.0008 | 0.002 |
Bud Periphery | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0008 | 0 | 0.0003 | 0.0002 | 0.0001 | 0 | 0.0006 | 0 | 0.0003 | 0.0003 | 0.0006 | 0.0001 |
Bud Site | 0.0009 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0035 | 0.0004 | 0.002 | 0.0002 | 0.0004 | 0.0001 | 0.0037 | 0.0003 | 0.0009 | 0.0008 | 0.0002 | 0.0002 |
Cell Periphery | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Cytoplasm | 0.3598 | 0.4646 | 0.3409 | 0.4245 | 0.3779 | 0.3788 | 0.3907 | 0.5723 | 0.3766 | 0.3955 | 0.5116 | 0.3848 | 0.425 | 0.6515 | 0.4898 | 0.6017 | 0.4679 | 0.4942 |
Cytoplasmic Foci | 0.0048 | 0.001 | 0.0031 | 0.0016 | 0.0011 | 0.0014 | 0.0176 | 0.0029 | 0.0017 | 0.0036 | 0.0131 | 0.0027 | 0.0144 | 0.0007 | 0.0077 | 0.0159 | 0.0025 | 0.0056 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0055 | 0.0024 | 0.0026 | 0.0019 | 0.0039 | 0.0021 | 0.0058 | 0.0022 | 0.0023 | 0.0064 | 0.0046 | 0.0034 | 0.0076 | 0.0012 | 0.0024 | 0.0028 | 0.0052 | 0.0031 |
Endosome | 0.0097 | 0.0017 | 0.0084 | 0.0012 | 0.0039 | 0.0021 | 0.0317 | 0.0035 | 0.0035 | 0.0108 | 0.0326 | 0.0039 | 0.0302 | 0.0005 | 0.0093 | 0.009 | 0.0344 | 0.0082 |
Golgi | 0.0008 | 0 | 0.0007 | 0 | 0 | 0 | 0.0059 | 0.0001 | 0.0004 | 0.0005 | 0.0145 | 0.0001 | 0.0102 | 0 | 0.0019 | 0.0037 | 0.0056 | 0.0043 |
Lipid Particles | 0.0016 | 0 | 0.0005 | 0 | 0 | 0 | 0.0049 | 0 | 0.0002 | 0.001 | 0.0197 | 0 | 0.0171 | 0 | 0.0013 | 0.0007 | 0.0033 | 0.0026 |
Mitochondria | 0.0022 | 0.0003 | 0.0005 | 0.0003 | 0.0004 | 0.0002 | 0.0074 | 0.0004 | 0.0018 | 0.0013 | 0.0075 | 0.0003 | 0.0064 | 0.0003 | 0.0011 | 0.0015 | 0.0022 | 0.0016 |
None | 0.0096 | 0.0016 | 0.0042 | 0.0056 | 0.0052 | 0.0023 | 0.0025 | 0.0015 | 0.001 | 0.0044 | 0.0017 | 0.001 | 0.0066 | 0.0009 | 0.002 | 0.005 | 0.0011 | 0.002 |
Nuclear Periphery | 0.0205 | 0.0155 | 0.0149 | 0.0139 | 0.0197 | 0.0147 | 0.0214 | 0.0115 | 0.015 | 0.0148 | 0.0191 | 0.0153 | 0.0196 | 0.0092 | 0.0133 | 0.0096 | 0.0233 | 0.0111 |
Nucleolus | 0.0008 | 0.0004 | 0.0005 | 0.0004 | 0.0006 | 0.0004 | 0.001 | 0.0002 | 0.0004 | 0.0003 | 0.0003 | 0.0002 | 0.0011 | 0.0001 | 0.0004 | 0.0002 | 0.0003 | 0.0003 |
Nucleus | 0.566 | 0.5072 | 0.6101 | 0.5378 | 0.5768 | 0.5908 | 0.4688 | 0.3989 | 0.5841 | 0.5504 | 0.3575 | 0.5804 | 0.4172 | 0.332 | 0.4546 | 0.3063 | 0.4387 | 0.45 |
Peroxisomes | 0.0011 | 0 | 0.0002 | 0 | 0 | 0 | 0.0077 | 0.0001 | 0.0005 | 0.0001 | 0.0023 | 0.0001 | 0.0073 | 0 | 0.0028 | 0.0065 | 0.0002 | 0.0017 |
Punctate Nuclear | 0.006 | 0.0022 | 0.0088 | 0.0103 | 0.0063 | 0.0031 | 0.0042 | 0.0034 | 0.0035 | 0.0027 | 0.0085 | 0.0043 | 0.012 | 0.0015 | 0.0056 | 0.0081 | 0.0052 | 0.0077 |
Vacuole | 0.0026 | 0.0016 | 0.0017 | 0.0006 | 0.0025 | 0.0017 | 0.0069 | 0.0012 | 0.0017 | 0.0042 | 0.0034 | 0.0013 | 0.0028 | 0.0006 | 0.0032 | 0.0013 | 0.0027 | 0.0012 |
Vacuole Periphery | 0.0005 | 0.0002 | 0.0003 | 0.0002 | 0.0005 | 0.0003 | 0.002 | 0.0003 | 0.0003 | 0.0019 | 0.0009 | 0.0003 | 0.0009 | 0.0001 | 0.0006 | 0.0003 | 0.0023 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 35.2261 | 22.2282 | 25.7723 | 31.1588 | 41.3064 | 33.1356 | 30.7859 | 30.0221 | 36.4563 | 37.6762 |
Translational Efficiency | 1.3091 | 1.6866 | 1.2866 | 1.3126 | 1.0116 | 1.1543 | 0.8833 | 0.8433 | 0.8306 | 0.951 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2037 | 1105 | 168 | 386 | 112 | 1660 | 1772 | 805 | 2149 | 2765 | 1940 | 1191 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 862.55 | 1075.45 | 1401.74 | 1136.34 | 1103.21 | 1044.55 | 1135.80 | 1216.24 | 875.09 | 1056.90 | 1158.83 | 1190.34 |
Standard Deviation | 128.47 | 187.14 | 187.35 | 132.89 | 155.05 | 169.70 | 156.19 | 149.07 | 140.56 | 177.52 | 175.83 | 148.80 |
Intensity Change Log 2 | — | 0.318260 | 0.700539 | 0.397715 | — | -0.078826 | 0.042001 | 0.140721 | — | 0.108977 | 0.368343 | 0.259157 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000165 | 0.000480 | 0.001549 | 0.000674 | 0.001811 | 0.000462 | 0.000445 | 0.000033 | 0.000251 | 0.000469 | 0.000541 | 0.000241 |
Bud Neck | 0.001314 | 0.004215 | 0.005511 | 0.002773 | 0.001139 | 0.002071 | 0.007020 | 0.005841 | 0.001305 | 0.002928 | 0.006889 | 0.004847 |
Bud Site | 0.002663 | 0.002568 | 0.002567 | 0.003173 | 0.003271 | 0.002501 | 0.006023 | 0.001371 | 0.002695 | 0.002528 | 0.005724 | 0.001955 |
Cell Periphery | 0.000081 | 0.000101 | 0.000226 | 0.000168 | 0.000496 | 0.000088 | 0.000148 | 0.000023 | 0.000103 | 0.000093 | 0.000154 | 0.000070 |
Cytoplasm | 0.566300* | 0.303912* | 0.071147 | 0.257821* | 0.342682* | 0.374939* | 0.228259* | 0.520930* | 0.554646* | 0.346554* | 0.214653* | 0.435657* |
Cytoplasmic Foci | 0.012236 | 0.003743 | 0.001352 | 0.004152 | 0.013195 | 0.006269 | 0.001376 | 0.000912 | 0.012286 | 0.005260 | 0.001374 | 0.001962 |
Eisosomes | 0.000009 | 0.000011 | 0.000029 | 0.000024 | 0.000055 | 0.000014 | 0.000013 | 0.000003 | 0.000011 | 0.000013 | 0.000015 | 0.000010 |
Endoplasmic Reticulum | 0.001152 | 0.002386 | 0.005570 | 0.006141 | 0.013899 | 0.002312 | 0.003701 | 0.000274 | 0.001816 | 0.002341 | 0.003863 | 0.002175 |
Endosome | 0.000615 | 0.000735 | 0.000817 | 0.001884 | 0.003164 | 0.001316 | 0.000716 | 0.000172 | 0.000748 | 0.001084 | 0.000724 | 0.000727 |
Golgi | 0.000613 | 0.000735 | 0.000169 | 0.000522 | 0.000382 | 0.001122 | 0.000124 | 0.000028 | 0.000601 | 0.000967 | 0.000128 | 0.000188 |
Lipid Particles | 0.000364 | 0.001319 | 0.000689 | 0.000925 | 0.002206 | 0.000582 | 0.000387 | 0.000090 | 0.000460 | 0.000877 | 0.000413 | 0.000361 |
Mitochondria | 0.000816 | 0.003242 | 0.001446 | 0.000716 | 0.000473 | 0.002111 | 0.001437 | 0.001029 | 0.000798 | 0.002563 | 0.001438 | 0.000927 |
Mitotic Spindle | 0.002559 | 0.006691 | 0.002478 | 0.003266 | 0.000282 | 0.005457 | 0.011238 | 0.002695 | 0.002440 | 0.005950 | 0.010479 | 0.002880 |
None | 0.016251 | 0.013538 | 0.003293 | 0.004632 | 0.003660 | 0.008721 | 0.008282 | 0.006440 | 0.015595 | 0.010646 | 0.007850 | 0.005854 |
Nuclear Periphery | 0.000237 | 0.000565 | 0.001496 | 0.001724 | 0.000724 | 0.000768 | 0.002018 | 0.000869 | 0.000263 | 0.000687 | 0.001973 | 0.001146 |
Nuclear Periphery Foci | 0.001302 | 0.001510 | 0.000825 | 0.003621 | 0.015937 | 0.001047 | 0.000981 | 0.000088 | 0.002064 | 0.001232 | 0.000967 | 0.001233 |
Nucleolus | 0.000616 | 0.001501 | 0.000320 | 0.000242 | 0.001243 | 0.001413 | 0.000261 | 0.000496 | 0.000649 | 0.001448 | 0.000266 | 0.000414 |
Nucleus | 0.384011* | 0.641422* | 0.877405* | 0.652039* | 0.550053* | 0.571545* | 0.708134* | 0.452106* | 0.392665* | 0.599470* | 0.722792* | 0.516904* |
Peroxisomes | 0.000251 | 0.000609 | 0.000156 | 0.000146 | 0.000431 | 0.000568 | 0.000193 | 0.000084 | 0.000260 | 0.000584 | 0.000190 | 0.000104 |
Vacuole | 0.008222 | 0.010298 | 0.022809 | 0.053313 | 0.044519 | 0.016279 | 0.018738 | 0.006340 | 0.010114 | 0.013889 | 0.019091 | 0.021564 |
Vacuole Periphery | 0.000222 | 0.000421 | 0.000145 | 0.002042 | 0.000378 | 0.000415 | 0.000504 | 0.000177 | 0.000230 | 0.000417 | 0.000473 | 0.000781 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.15 | -8.86 | -6.65 | -2.86 | 6.18 | 4.93 | 4.92 | 7.12 | 2.79 | 17.36* | -1.79 | -6.10 | -0.61 | 1.69 | 9.46 |
Bud Neck | -2.75 | -7.91 | -6.03 | -1.05 | 3.86 | -3.57 | -7.40 | -5.04 | -3.93 | 1.34 | -3.55 | -8.01 | -6.34 | -3.63 | 2.10 |
Bud Site | -0.14 | -2.60 | -2.31 | -2.32 | 0.30 | 1.69 | -1.79 | 3.92 | 1.77 | 4.24 | 0.16 | -2.72 | 0.37 | 0.27 | 3.34 |
Cell Periphery | -2.10 | -7.63 | -5.65 | -4.62 | 4.54 | 7.19 | 6.14 | 8.36 | 12.16 | 18.51 | 0.63 | -5.29 | 2.36 | 2.89 | 10.27 |
Cytoplasm | 28.85 | 49.55 | 31.91 | 6.93 | -12.02 | -0.91 | 6.32 | -5.06 | -9.74 | -28.52 | 28.91 | 54.74 | 16.97 | -5.74 | -24.81 |
Cytoplasmic Foci | 7.84 | 12.97 | 8.85 | 0.13 | -4.28 | 3.25 | 6.32 | 6.45 | 10.61 | 1.37 | 7.76 | 14.58 | 13.37 | 7.93 | -3.13 |
Eisosomes | -3.62 | -8.62 | -9.96 | -7.96 | 2.76 | 5.98 | 5.44 | 7.74 | 13.80 | 19.39 | -3.58 | -10.02 | -2.27 | 0.64 | 6.60 |
Endoplasmic Reticulum | -8.39 | -10.10 | -11.99 | -8.26 | 1.33 | 7.38 | 5.98 | 8.90 | 16.26 | 20.96 | -5.54 | -14.02 | -3.41 | 0.66 | 9.19 |
Endosome | -1.15 | -4.03 | -5.73 | -4.90 | -2.23 | 3.84 | 5.41 | 6.60 | 4.86 | 8.73 | -1.96 | -0.23 | -0.06 | 1.93 | 0.16 |
Golgi | -0.15 | 1.36 | 1.10 | 1.21 | -0.08 | -1.19 | 4.77 | 6.20 | 2.02 | 5.14 | -0.62 | 2.13 | 2.11 | 2.42 | 0.12 |
Lipid Particles | -1.52 | -6.10 | -8.18 | -0.32 | -0.38 | 3.77 | 3.92 | 5.02 | 5.89 | 13.47 | -1.70 | -3.18 | -0.95 | 1.45 | 2.34 |
Mitochondria | -1.61 | -3.08 | -0.43 | 1.55 | 7.38 | -2.07 | -10.42 | -3.88 | 1.15 | 3.12 | -2.00 | -2.27 | -0.65 | 1.91 | 4.56 |
Mitotic Spindle | -1.95 | -0.31 | -1.95 | -0.59 | -1.80 | -3.93 | -6.14 | -2.41 | 1.77 | 3.99 | -2.42 | -4.01 | -1.33 | 0.92 | 2.65 |
None | 2.30 | 10.68 | 8.22 | 5.79 | -3.34 | -4.83 | -4.20 | -6.23 | 1.26 | -0.45 | 4.34 | 7.32 | 7.79 | 4.80 | -0.06 |
Nuclear Periphery | -8.50 | -10.13 | -14.41 | -11.85 | -0.79 | -1.55 | -11.98 | -3.77 | -2.87 | 10.09 | -15.20 | -18.10 | -18.57 | -10.84 | 6.37 |
Nuclear Periphery Foci | -1.46 | 0.19 | -5.40 | -3.74 | -5.13 | 3.69 | 3.74 | 4.00 | 10.33 | 5.01 | 1.92 | 3.06 | 2.32 | 0.63 | -0.99 |
Nucleolus | -1.68 | 0.33 | 1.84 | 2.22 | 2.51 | -0.43 | 1.88 | 1.52 | 1.67 | -0.25 | -2.06 | 1.45 | 0.99 | 2.45 | -0.13 |
Nucleus | -25.99 | -31.53 | -17.14 | 0.91 | 11.37 | -1.12 | -7.34 | 1.69 | 6.72 | 22.91 | -26.65 | -46.64 | -15.06 | 6.64 | 22.71 |
Peroxisomes | -1.81 | -0.41 | 0.01 | 1.59 | 0.21 | -0.39 | 3.17 | 4.59 | 2.42 | 0.97 | -2.05 | 1.56 | 2.09 | 3.04 | 0.30 |
Vacuole | -4.53 | -5.69 | -11.16 | -10.32 | -4.76 | 3.31 | 2.38 | 5.44 | 8.00 | 10.16 | -7.88 | -11.37 | -9.14 | -5.64 | -1.61 |
Vacuole Periphery | -0.99 | -0.21 | -0.90 | -0.67 | -0.89 | 0.10 | 0.65 | 2.08 | 1.41 | 1.10 | -1.31 | -0.57 | -0.62 | -0.04 | -0.35 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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