Standard name
Human Ortholog
Description ABC type transmembrane transporter of MRP/CFTR family; found in vacuolar membrane, involved in the transport of unconjugated bilirubin and in heavy metal detoxification via glutathione conjugates, along with Ycf1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Cytoplasm 0.07 0.08 0.11 0.28 0.34 0.38 0.37 0.5 0.55 0.48 0.54 0.56 0.06 0.05 0.05 0.35 – – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 – – 0 0 0 0 0 0
Endosome 0.12 0 0.15 0.05 0.11 0 0 0 0 0 0 0 0.08 0.09 0.11 0 – – 0.05 0.05 0.07 0.13 0.12 0.12
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Mitochondria 0 0 0.09 0.4 0.25 0.62 0.71 0.57 0.61 0.66 0.59 0.64 0 0 0 0.12 – – 0 0 0 0 0 0
Nucleus 0.09 0.06 0 0 0 0 0 0 0 0 0 0 0.11 0.11 0.09 0 – – 0 0 0 0 0 0
Nuclear Periphery 0 0.06 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0.12 0.05 0.05 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Vac/Vac Membrane 0.83 0.89 0.75 0.52 0.55 0.3 0.26 0.19 0.18 0.18 0.16 0.2 0.83 0.85 0.85 0.59 – – 0.84 0.87 0.74 0.73 0.75 0.77
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 2 0 0 0 1 0 0 0 0 1 0 1 0 – – 1 0 0 0 0 1
Bud 0 1 0 0 0 4 3 7 7 2 8 10 0 0 1 0 – – 2 2 1 0 5 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 1 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – –
Cell Periphery 0 0 0 0 0 1 4 2 2 1 2 3 0 0 0 0 – – 0 0 0 0 0 0
Cytoplasm 41 34 32 42 57 110 119 214 159 166 144 167 22 19 17 6 – – 5 3 4 3 3 3
Endoplasmic Reticulum 3 2 7 5 1 7 7 7 4 2 5 2 11 6 7 2 – – 0 1 5 3 3 1
Endosome 65 12 43 8 19 5 0 3 3 1 0 0 29 36 39 0 – – 29 24 20 55 62 62
Golgi 1 0 1 0 2 0 0 0 0 0 0 0 0 0 4 0 – – 6 0 3 1 2 3
Mitochondria 11 13 26 60 42 177 226 242 176 230 156 189 0 0 0 2 – – 9 4 6 9 10 7
Nucleus 52 26 11 2 5 2 4 8 1 4 1 3 39 45 34 0 – – 11 8 1 10 10 7
Nuclear Periphery 11 24 21 5 0 3 5 2 3 3 1 3 6 17 4 0 – – 22 10 35 23 24 21
Nucleolus 9 0 1 0 0 0 0 0 0 0 0 0 3 2 5 0 – – 2 1 0 3 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
SpindlePole 1 0 1 0 0 2 0 1 0 1 1 0 2 2 4 0 – – 1 0 0 0 3 1
Vac/Vac Membrane 470 362 211 78 93 86 84 81 52 61 43 60 305 349 314 10 – – 519 381 227 320 405 403
Unique Cell Count 565 409 282 151 169 287 320 427 287 346 265 297 366 412 370 17 620 439 308 436 537 525
Labelled Cell Count 664 474 354 202 219 397 452 568 407 471 361 437 418 476 430 20 620 439 308 436 537 525


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.3 7.4 5.7 5.3 5.2 4.4 4.3 4.4 4.1 4.0 4.0 4.1 7.7 7.8 8.1 7.3 – – 7.6 8.0 8.5
Std Deviation (1e-4) 1.3 1.1 1.0 1.1 1.1 1.2 1.5 1.2 0.9 1.0 1.0 1.0 1.0 1.0 1.1 1.4 – – 1.4 1.6 1.8
Intensity Change (Log2) – – – -0.13 -0.13 -0.39 -0.42 -0.37 -0.48 -0.52 -0.54 -0.48 0.43 0.45 0.5 0.34 – – 0.4 0.49 0.57


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Cytoplasm 4.3 5.8 7.4 7.3 10.6 11.1 9.8 10.8 11.4 -2.4 -3.3 -3.2 0 – –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0.4 -1.0 -0.5 0 – –
Endosome -3.1 -1.2 0 0 0 0 0 0 0 -2.9 -2.7 -1.8 0 – –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Mitochondria 7.6 4.5 0 0 0 0 0 0 0 -5.9 -6.3 -6.0 0 – –
Nucleus 0 -0.5 -2.6 -2.1 -1.6 -2.9 -2.2 -2.8 -2.3 3.2 3.3 2.6 0 – –
Nuclear Periphery -1.7 -3.6 0 0 0 0 0 0 0 -3.7 -1.9 -4.2 0 – –
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Vacuole -4.9 -4.3 -10.7 -11.9 -14.8 -13.6 -14.4 -13.7 -13.2 2.7 3.2 3.2 -1.5 – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.7807 2.9744 2.8221 2.5562 2.2054 2.7639 4.9632 5.2664 5.0536 5.0952 4.1319 5.0288 5.5688 6.5583 5.0549 5.2796 4.4363 4.6762
Actin 0.0245 0 0.0077 0.0468 0.0157 0.0027 0.0244 0 0.0027 0 0 0.0005 0.1184 0 0.018 0.0001 0 0
Bud 0.0008 0.0004 0.0006 0.0008 0.0017 0.0003 0.0006 0.0004 0.0082 0.0003 0.0001 0.0021 0.0025 0.0002 0.0021 0.0005 0.0003 0.001
Bud Neck 0.0027 0.0002 0.002 0.0024 0.0012 0.0003 0.0037 0.0002 0.0005 0.0002 0.0001 0.0011 0.0012 0.0002 0.0014 0.0006 0.0002 0.0006
Bud Periphery 0.0009 0.0008 0.0008 0.0022 0.0022 0.0004 0.001 0.0008 0.0041 0.0008 0.0002 0.0015 0.0036 0.0003 0.0024 0.0008 0.0004 0.0017
Bud Site 0.0029 0.0006 0.0021 0.002 0.0194 0.0003 0.0009 0.002 0.0033 0.0002 0.0001 0.0009 0.003 0.0007 0.0318 0.0014 0.0007 0.0005
Cell Periphery 0.0006 0.0005 0.0004 0.0003 0.0003 0.0002 0.0006 0.0005 0.0007 0.0002 0.0002 0.0004 0.0012 0.0006 0.0023 0.0006 0.0003 0.0009
Cytoplasm 0.013 0.0171 0.0214 0.0046 0.0031 0.0109 0.0149 0.0092 0.028 0.0074 0.001 0.0178 0.0108 0.0179 0.0294 0.0043 0.0162 0.0094
Cytoplasmic Foci 0.0147 0.0035 0.0092 0.0104 0.019 0.0046 0.0151 0.0054 0.0152 0.0054 0.0015 0.0151 0.0188 0.005 0.0165 0.008 0.0023 0.0064
Eisosomes 0.0003 0 0 0.0007 0.0002 0 0.0005 0 0 0 0 0 0.0008 0 0.0003 0 0 0
Endoplasmic Reticulum 0.0064 0.0036 0.0058 0.0032 0.0013 0.0083 0.0057 0.0063 0.0044 0.0017 0.0008 0.0026 0.0307 0.0028 0.0067 0.0013 0.0011 0.0041
Endosome 0.2178 0.1084 0.2208 0.1231 0.1603 0.167 0.2373 0.1565 0.2167 0.262 0.1455 0.2265 0.2528 0.1431 0.195 0.2199 0.1093 0.1252
Golgi 0.0101 0.0013 0.0061 0.0083 0.0079 0.0036 0.0148 0.0016 0.0044 0.0028 0.0012 0.0031 0.1063 0.0047 0.0173 0.0062 0.0009 0.0019
Lipid Particles 0.0129 0.0005 0.0046 0.0105 0.0115 0.0034 0.0119 0.0014 0.0035 0.0005 0.0005 0.0035 0.0149 0.0014 0.0033 0.0018 0.0005 0.0043
Mitochondria 0.0087 0.0066 0.0105 0.0185 0.0067 0.0066 0.0149 0.0052 0.009 0.0316 0.0011 0.0063 0.0261 0.0012 0.0511 0.0019 0.0006 0.0066
None 0.0074 0.001 0.003 0.008 0.0069 0.0003 0.0208 0.0099 0.0126 0.0002 0.0001 0.0049 0.004 0.0026 0.0032 0.0002 0.0001 0.0172
Nuclear Periphery 0.0341 0.0252 0.0191 0.0098 0.0071 0.03 0.0169 0.0397 0.0068 0.0047 0.0063 0.0146 0.0472 0.0089 0.0057 0.0019 0.0079 0.0483
Nucleolus 0.0025 0.0011 0.0016 0.0011 0.0019 0.0005 0.0044 0.0009 0.0019 0.0002 0.0003 0.0017 0.0006 0.0008 0.0037 0.0008 0.0007 0.002
Nucleus 0.0162 0.0188 0.0049 0.0028 0.0022 0.0064 0.0174 0.0038 0.0054 0.0009 0.0007 0.0049 0.0083 0.0034 0.0038 0.0013 0.0032 0.0031
Peroxisomes 0.0052 0.0001 0.006 0.005 0.0158 0.0018 0.0073 0.0002 0.0014 0.0001 0.0001 0.0007 0.0022 0.0001 0.0022 0.0005 0.0001 0.001
Punctate Nuclear 0.0033 0.0001 0.0006 0.0094 0.0162 0.0013 0.0041 0.0001 0.0039 0 0 0.0007 0.002 0.0002 0.0004 0.0001 0 0.0001
Vacuole 0.3745 0.4702 0.4111 0.4238 0.3604 0.3687 0.3893 0.4947 0.4371 0.3619 0.4157 0.4091 0.2649 0.586 0.473 0.5555 0.4556 0.4579
Vacuole Periphery 0.2406 0.3402 0.2616 0.3062 0.3391 0.3824 0.1934 0.2612 0.2301 0.319 0.4246 0.2821 0.0796 0.2198 0.1304 0.1922 0.3996 0.308

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.0445 13.0674 16.4216 21.2031 19.2582 20.265 16.7286 19.8486 22.2607 24.0667
Translational Efficiency 0.525 0.6152 0.6177 0.5099 0.4267 0.6578 0.5139 0.4392 0.4896 0.4421

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1948 1574 1444 416 1391 1048 1805 907 3339 2622 3249 1323

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 809.01 883.80 910.56 1032.68 630.92 844.78 931.34 976.06 734.82 868.20 922.10 993.86
Standard Deviation 100.48 126.67 136.67 160.84 91.47 119.28 125.96 138.76 130.71 125.24 131.24 148.41
Intensity Change Log 2 — 0.127562 0.170597 0.352164 — 0.421119 0.561851 0.629513 — 0.263589 0.355196 0.480292

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000147 0.000019 0.000081 0.000105 0.000091 0.000088 0.000037 0.000064 0.000124 0.000047 0.000056 0.000077
Bud Neck 0.007829 0.001240 0.012784 0.005395 0.010358 0.003696 0.003313 0.005837 0.008883 0.002222 0.007523 0.005698
Bud Site 0.014544 0.005232 0.004705 0.028695 0.021101 0.003700 0.004700 0.010353 0.017276 0.004620 0.004702 0.016120
Cell Periphery 0.000110 0.000070 0.000593 0.000104 0.000659 0.000224 0.000085 0.000079 0.000339 0.000132 0.000311 0.000087
Cytoplasm 0.016096 0.013238 0.008818 0.018600 0.085387 0.006155 0.003389 0.003879 0.044962 0.010407 0.005802 0.008508
Cytoplasmic Foci 0.012172 0.027002 0.002161 0.016106 0.113830 0.010943 0.002664 0.010657 0.054522 0.020583 0.002440 0.012370
Eisosomes 0.000015 0.000006 0.000050 0.000009 0.000107 0.000014 0.000010 0.000011 0.000053 0.000009 0.000028 0.000011
Endoplasmic Reticulum 0.002631 0.001958 0.005925 0.001477 0.025570 0.005817 0.002185 0.001973 0.012187 0.003500 0.003847 0.001817
Endosome 0.090801 0.071121 0.025219 0.058089 0.074866 0.051087 0.033825 0.078148 0.084163 0.063113 0.030000 0.071840
Golgi 0.005397 0.009802 0.000835 0.008916 0.011788 0.004565 0.003355 0.010871 0.008060 0.007709 0.002235 0.010256
Lipid Particles 0.000991 0.000775 0.001698 0.000369 0.006709 0.003833 0.000285 0.000615 0.003373 0.001997 0.000913 0.000537
Mitochondria 0.007888 0.006342 0.004594 0.002623 0.061408 0.016525 0.002236 0.003869 0.030184 0.010412 0.003284 0.003477
Mitotic Spindle 0.001169 0.003250 0.001331 0.006298 0.000350 0.000879 0.001263 0.003558 0.000828 0.002302 0.001294 0.004419
None 0.000136 0.000415 0.002457 0.000173 0.003303 0.001775 0.000249 0.000175 0.001455 0.000959 0.001230 0.000174
Nuclear Periphery 0.001427 0.000748 0.006359 0.000761 0.000407 0.002581 0.001603 0.001623 0.001002 0.001481 0.003717 0.001352
Nuclear Periphery Foci 0.000398 0.000922 0.002200 0.001402 0.000219 0.001460 0.000700 0.001987 0.000323 0.001137 0.001366 0.001803
Nucleolus 0.002433 0.001086 0.021727 0.001290 0.000385 0.003569 0.004971 0.003597 0.001580 0.002078 0.012418 0.002872
Nucleus 0.010314 0.002375 0.086047 0.003671 0.004650 0.006546 0.010814 0.006040 0.007955 0.004042 0.044251 0.005295
Peroxisomes 0.001135 0.000617 0.001105 0.001292 0.001141 0.001271 0.000462 0.001185 0.001138 0.000878 0.000748 0.001219
Vacuole 0.697464* 0.395412* 0.442125* 0.656299* 0.270282* 0.493459* 0.605486* 0.581301* 0.519504* 0.434601* 0.532882* 0.604883*
Vacuole Periphery 0.126902* 0.458369* 0.369185* 0.188327* 0.307389* 0.381813* 0.318366* 0.274180* 0.202091* 0.427770* 0.340952* 0.247185*

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.02 1.03 0.58 -2.55 -0.70 0.07 1.78 0.76 0.54 -1.74 1.85 1.71 1.09 -1.48 -1.35
Bud Neck 10.77 -3.27 2.58 -6.99 4.77 4.83 6.70 3.25 -1.69 -2.61 10.04 1.62 3.60 -4.74 1.91
Bud Site 6.29 6.27 -3.17 -5.44 -5.49 12.53 11.31 6.11 -5.12 -4.10 12.10 11.64 0.59 -6.98 -6.79
Cell Periphery 2.15 -3.49 0.26 -1.95 3.54 5.40 11.69 11.80 2.27 0.80 5.95 0.44 10.38 1.69 3.65
Cytoplasm 1.56 3.73 -1.24 -2.29 -3.72 19.94 21.45 20.78 0.93 -0.92 16.17 19.41 16.16 0.54 -2.76
Cytoplasmic Foci -8.64 12.47 -1.92 4.41 -7.17 28.52* 32.16 28.63 0.24 -7.37 16.74 29.87 21.34 5.94 -10.31
Eisosomes 6.32 -8.65 3.66 -2.75 10.30 11.86 12.48 12.25 1.35 -1.29 12.76 6.52 12.25 -1.79 8.97
Endoplasmic Reticulum 2.50 -7.16 3.20 1.37 9.29 10.58 13.01 12.99 6.31 0.58 10.65 10.35 12.73 5.13 6.78
Endosome 4.29 19.83 5.89 2.34 -6.43 5.92 15.05 -0.68 -5.20 -10.41 6.64 24.25 3.39 -2.09 -12.59
Golgi -3.57 5.32 -1.82 0.59 -4.81 6.27 9.61 0.57 -3.47 -4.58 0.36 9.00 -1.60 -1.79 -6.52
Lipid Particles 0.82 -3.17 3.49 1.62 8.09 2.14 7.86 7.33 3.03 -2.20 2.41 6.83 7.62 3.19 2.77
Mitochondria 1.16 2.74 3.31 2.74 1.58 12.42 19.55 18.82 6.13 -2.58 11.37 17.87 17.05 6.62 -0.29
Mitotic Spindle -3.11 -0.33 -2.57 -1.73 -2.47 -1.57 -3.19 -3.74 -2.95 -2.43 -3.48 -1.79 -4.06 -2.46 -3.44
None -3.56 -4.50 -0.52 2.32 4.39 1.17 2.57 2.64 3.00 0.80 0.91 0.39 2.58 3.56 4.45
Nuclear Periphery 1.85 -5.73 1.59 -0.48 6.98 -5.31 -3.50 -4.63 1.96 -0.08 -1.65 -6.04 -1.36 0.32 5.37
Nuclear Periphery Foci -3.02 -2.95 -2.69 -1.48 0.81 -2.67 -2.86 -3.59 -0.82 -2.53 -3.83 -3.61 -4.21 -1.79 -1.08
Nucleolus 3.84 -11.67 2.79 -0.68 12.35 -3.68 -8.73 -4.98 -0.07 1.62 -1.24 -13.36 -2.73 -1.41 10.41
Nucleus 6.80 -14.49 4.84 -1.58 15.92 -1.40 -4.56 -1.54 0.14 3.04 4.52 -14.21 2.61 -1.74 15.25
Peroxisomes 2.86 0.24 -0.39 -2.00 -0.52 -0.52 4.94 -0.23 0.31 -3.80 1.67 3.70 -0.41 -1.64 -2.84
Vacuole 39.27* 27.71 3.14 -20.49 -15.67 -22.50 -42.25 -32.27 -8.26 2.67 11.87 -1.91 -10.45 -21.74 -9.34
Vacuole Periphery -44.54* -26.12* -5.58 23.53* 14.35 -7.34 -1.24 3.56 10.25 5.07 -35.85 -22.94 -6.07 23.28 12.52
Standard name
Human Ortholog
Description ABC type transmembrane transporter of MRP/CFTR family; found in vacuolar membrane, involved in the transport of unconjugated bilirubin and in heavy metal detoxification via glutathione conjugates, along with Ycf1p
Localization
Cell Percentages vacuolar membrane (43%)
Cell Cycle Regulation No
Subcompartmental Group vac memb-1

Bpt1

Bpt1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bpt1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available