Standard name
Human Ortholog
Description Centrin (Cdc31p)-binding protein required for SPB duplication; localizes to the half-bridge of the spindle pole body (SPB); required for progression through G(2)-M transition; phosphorylated by Cdc28p-Clb2p and by Cdc5p; dephosphorylated by Cdc14p; has similarity to Xenopus laevis XCAP-C
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.08 0.13 0.1 0.14 0.16 0.17 0.19 0.17 0.16 0.21 0 0 0.08 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.12 0.1 0.12 0.11 0.09 0.1 0.09 0.08 0.07 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0.05 0.05 0 0.1 0.08 0.08 0.07 0.07 0.16 0.11 0.12 0.15 0 0 0 0 0.05 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0.12 0.07 0.11 0.11 0.13 0.13 0.1 0.1 0.11 0.11 0.1 0.11 0.19 0.27 0.2 0.11 0.21 0 0 0 0.06 0.06 0.09
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.07 0.11 0 0 0 0 0 0 0 0 0 0 0.17 0.19 0.15 0.09 0.23 0.09 0.06 0.11 0.08 0 0.06 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.2 0.09 0.17 0.06 0.1 0.13 0.12 0.17 0.24 0.3 0.25 0 0 0.07 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0.12 0 0 0 0 0 0 0.05 0 0 0 0.14 0.14 0.2 0 0.08 0 0 0 0 0 0 0
SpindlePole 0.9 0.59 0.79 0.7 0.78 0.73 0.74 0.77 0.74 0.67 0.66 0.68 0.75 0.65 0.49 0.71 0.76 0.75 0.88 0.71 0.78 0.79 0.73 0.71
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 1 6 12 2 4 2 3 1 4 3 1 0 3 4 1 3 0 1 1 3 1 4 9
Bud 7 3 20 41 40 65 82 99 74 76 57 102 6 9 30 1 3 2 0 1 0 1 6 9
Bud Neck 5 0 30 32 50 52 45 58 37 37 24 37 4 6 1 4 4 3 0 1 1 1 4 7
Bud Site 13 4 10 32 33 36 36 39 63 47 42 73 8 3 12 6 8 8 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 14 9 16 36 47 60 68 60 39 49 39 51 25 71 98 41 18 46 3 2 5 7 15 27
Endoplasmic Reticulum 1 0 1 0 0 0 1 1 1 1 0 0 0 2 1 1 1 1 0 0 1 0 1 1
Endosome 20 8 1 0 4 3 2 1 3 0 0 0 38 70 54 19 37 20 15 11 23 4 16 14
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 1 1
Mitochondria 4 15 21 53 25 47 69 68 67 106 103 125 7 5 24 2 4 2 5 4 11 2 3 10
Nucleus 1 0 0 0 0 2 3 2 1 0 1 0 0 0 0 1 0 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 1 0 0 1 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 5 1 0 0 0 7 3 1 3 2 5 9 3 7 1 1 0 3 0 0 0 0 1 0
Peroxisomes 4 9 1 3 8 9 15 5 19 10 9 19 31 51 72 7 13 6 2 3 5 2 8 5
SpindlePole 241 45 193 222 327 341 386 447 289 297 230 335 166 240 181 143 120 167 237 73 219 96 186 227
Vac/Vac Membrane 2 1 2 0 0 0 0 0 1 0 0 1 1 0 0 1 0 0 0 1 1 0 2 1
Unique Cell Count 268 76 244 317 417 467 525 582 392 444 349 494 221 369 366 202 158 223 271 103 280 122 254 320
Labelled Cell Count 319 96 301 432 537 626 712 785 598 629 514 753 290 467 478 229 211 261 271 103 280 122 254 320


SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 3.4 3.2 3.0 3.3 3.1 3.0 2.9 2.6 2.5 2.6 2.3 4.3 4.4 3.8 5.2 5.7 5.9 4.3 4.4 4.7
Std Deviation (1e-4) 0.9 0.6 0.5 0.8 1.0 1.2 2.5 1.1 1.2 0.8 1.2 0.8 1.1 1.3 1.0 1.1 1.5 1.9 1.0 1.6 1.3
Intensity Change (Log2) – – – -0.06 0.06 -0.04 -0.08 -0.11 -0.31 -0.36 -0.27 -0.45 0.44 0.47 0.26 0.73 0.84 0.91 0.44 0.46 0.57


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.3752 0.4345 0.2571 0.4173 0.2838 0.3853 0.8178 0.7478 0.899 1.0346 0.01 0.8511 0.5111 0.4972 0.6715 0.6641 1.3122 0.3907
Actin 0.007 0.0118 0.0016 0.056 0.0123 0.0018 0.0346 0.002 0.0155 0.0327 0.0008 0.0023 0.0042 0.0007 0.0046 0.0011 0.0772 0.0054
Bud 0.0002 0.0002 0.0002 0.0002 0.0002 0.0001 0.0006 0.0015 0.0002 0.0012 0.0001 0.0007 0.0001 0.0001 0.0001 0 0.0019 0.0001
Bud Neck 0.003 0.007 0.0006 0.0504 0.0007 0.0059 0.0024 0.0005 0.0019 0.0021 0.0004 0.0018 0.0003 0.0004 0.0022 0.0003 0.0011 0.0004
Bud Periphery 0.0003 0.0003 0.0003 0.0003 0.0008 0.0002 0.0008 0.0009 0.0003 0.002 0.0001 0.0006 0.0002 0.0001 0.0001 0 0.0051 0.0002
Bud Site 0.0092 0.0253 0.0006 0.0014 0.0005 0.0002 0.003 0.0083 0.002 0.0053 0.0005 0.0003 0.0002 0.0015 0.0016 0 0.0063 0.0005
Cell Periphery 0.0001 0.0004 0.0001 0.0001 0.0001 0 0.0003 0.0001 0.0001 0.0003 0 0.0001 0 0 0 0 0.0003 0
Cytoplasm 0.0021 0.0103 0.0031 0.0011 0.0075 0.002 0.0041 0.0274 0.0116 0.0057 0.0196 0.0041 0.001 0.0077 0.0048 0.004 0.0041 0.0074
Cytoplasmic Foci 0.3979 0.3771 0.4109 0.3151 0.4515 0.2496 0.3957 0.3527 0.4546 0.459 0.3788 0.2553 0.4331 0.2482 0.4804 0.4255 0.4165 0.3373
Eisosomes 0.0001 0.0003 0.0001 0.0006 0.0002 0 0.0006 0 0.0001 0.0002 0 0.0001 0 0 0.0001 0.0001 0.0011 0.0001
Endoplasmic Reticulum 0.0013 0.0007 0.0003 0.0002 0.0008 0.0004 0.0014 0.0009 0.0032 0.0018 0.0016 0.0002 0.0012 0.001 0.0004 0.0001 0.0011 0.0002
Endosome 0.0186 0.0186 0.0072 0.0047 0.0145 0.011 0.0187 0.0111 0.0181 0.0257 0.0727 0.0048 0.0074 0.015 0.0127 0.0023 0.012 0.0044
Golgi 0.0078 0.0044 0.0033 0.0062 0.0081 0.0029 0.0055 0.0029 0.0062 0.0231 0.0095 0.0035 0.0033 0.0023 0.0023 0.0018 0.0104 0.0025
Lipid Particles 0.0708 0.057 0.0448 0.0549 0.1143 0.0418 0.0312 0.0146 0.0293 0.024 0.0201 0.0251 0.0419 0.0209 0.0285 0.0226 0.1533 0.0354
Mitochondria 0.0028 0.0002 0.0093 0.0073 0.0482 0.0017 0.0107 0.0004 0.0043 0.0132 0.0026 0.0072 0.0014 0.0002 0.0004 0.0001 0.039 0.0006
None 0.005 0.0194 0.0529 0.0383 0.0393 0.0071 0.0194 0.0636 0.0182 0.0316 0.0237 0.0144 0.0169 0.1033 0.0952 0.2374 0.0671 0.0421
Nuclear Periphery 0.01 0.0007 0.0022 0.0003 0.0134 0.0054 0.0145 0.0012 0.0048 0.0027 0.0031 0.0019 0.0122 0.0083 0.0011 0.0002 0.0014 0.0005
Nucleolus 0.0029 0.0011 0.0008 0.0004 0.001 0.0009 0.0109 0.0019 0.0013 0.0012 0.0007 0.0015 0.0004 0.0009 0.0006 0.0002 0.0007 0.0007
Nucleus 0.0017 0.0019 0.0018 0.0002 0.0007 0.0012 0.0059 0.0031 0.0014 0.0014 0.0033 0.0009 0.0012 0.0019 0.0007 0.0001 0.0005 0.0006
Peroxisomes 0.0726 0.069 0.0505 0.0887 0.0717 0.0335 0.0494 0.0512 0.0512 0.0366 0.0575 0.0257 0.0739 0.0692 0.0696 0.0321 0.1087 0.0393
Punctate Nuclear 0.3857 0.3932 0.4088 0.3731 0.2109 0.6332 0.3882 0.4539 0.3752 0.3285 0.4017 0.649 0.3986 0.5174 0.2944 0.2718 0.0915 0.5223
Vacuole 0.0006 0.0011 0.0004 0.0002 0.0013 0.0006 0.0014 0.0016 0.0005 0.0012 0.0019 0.0003 0.0006 0.0006 0.0002 0 0.0004 0.0001
Vacuole Periphery 0.0005 0.0001 0.0004 0.0002 0.0022 0.0004 0.0007 0.0001 0.0003 0.0005 0.0013 0.0003 0.0018 0.0002 0.0001 0 0.0003 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.7387 7.9854 5.9255 8.5139 7.8052 10.0924 11.058 10.5435 9.7086 10.7992
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1882 1308 245 687 1407 1919 603 77 3289 3227 848 764

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 624.31 718.53 962.07 774.15 592.55 706.40 900.46 849.87 610.72 711.32 918.26 781.78
Standard Deviation 67.56 611.57 114.30 103.03 104.25 90.56 96.10 117.17 86.65 395.62 105.46 107.00
Intensity Change Log 2 — 0.202786 0.623879 0.310351 — 0.253548 0.603725 0.520305 — 0.227728 0.614100 0.416406

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000147 0.000375 0.001235 0.001255 0.000116 0.000582 0.000368 0.000656 0.000134 0.000498 0.000618 0.001195
Bud Neck 0.008408 0.003592 0.003008 0.005302 0.007963 0.007697 0.003857 0.005345 0.008218 0.006034 0.003612 0.005307
Bud Site 0.006729 0.005084 0.022303 0.031667 0.002274 0.004387 0.008025 0.085539 0.004823 0.004669 0.012150 0.037096
Cell Periphery 0.000087 0.000114 0.000200 0.001029 0.000136 0.000061 0.000088 0.000081 0.000108 0.000083 0.000120 0.000934
Cytoplasm 0.106675* 0.067691 0.154992* 0.329351* 0.104139* 0.054535 0.070498 0.103084* 0.105590* 0.059868 0.094910 0.306547*
Cytoplasmic Foci 0.620966* 0.466173* 0.191750* 0.107163 0.545241* 0.482124* 0.054575 0.205121* 0.588572* 0.475659* 0.094207 0.117036
Eisosomes 0.000075 0.000066 0.000074 0.000107 0.000127 0.000051 0.000054 0.000067 0.000097 0.000057 0.000060 0.000103
Endoplasmic Reticulum 0.000293 0.000361 0.002278 0.000810 0.000460 0.000231 0.001587 0.000477 0.000364 0.000284 0.001786 0.000776
Endosome 0.029744 0.055445 0.046953 0.030583 0.033377 0.063221 0.013877 0.021214 0.031298 0.060069 0.023433 0.029639
Golgi 0.011773 0.024132 0.002845 0.011899 0.013173 0.021600 0.000562 0.001189 0.012372 0.022627 0.001221 0.010819
Lipid Particles 0.037532 0.053520 0.016991 0.002691 0.089912 0.074251 0.016387 0.003479 0.059940 0.065848 0.016562 0.002771
Mitochondria 0.005814 0.005730 0.000129 0.005588 0.006193 0.007329 0.000922 0.000063 0.005976 0.006681 0.000693 0.005031
Mitotic Spindle 0.038074 0.148105* 0.284720* 0.263351* 0.034523 0.144533* 0.643262* 0.389268* 0.036554 0.145981* 0.539674* 0.276042*
None 0.017815 0.005606 0.000801 0.012893 0.027539 0.002407 0.002087 0.001247 0.021975 0.003704 0.001715 0.011720
Nuclear Periphery 0.000307 0.000444 0.000531 0.000561 0.000758 0.000303 0.001142 0.000246 0.000500 0.000361 0.000966 0.000530
Nuclear Periphery Foci 0.009396 0.019739 0.132186 0.005714 0.016028 0.009230 0.076277 0.021991 0.012233 0.013490 0.092430 0.007354
Nucleolus 0.013465 0.008536 0.003365 0.003948 0.011765 0.007560 0.006753 0.001171 0.012738 0.007956 0.005774 0.003668
Nucleus 0.021655 0.019528 0.021704 0.102168 0.035798 0.007823 0.059784 0.004895 0.027705 0.012568 0.048782 0.092364
Peroxisomes 0.065630 0.107693* 0.033307 0.018893 0.066268 0.101380* 0.010894 0.043351 0.065903 0.103939* 0.017369 0.021358
Vacuole 0.004956 0.007348 0.080247 0.061297 0.003852 0.008500 0.028677 0.111391 0.004484 0.008033 0.043576 0.066346
Vacuole Periphery 0.000460 0.000716 0.000382 0.003728 0.000358 0.002192 0.000324 0.000125 0.000416 0.001594 0.000341 0.003365

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.14 -7.84 -5.00 -3.77 2.39 -1.50 -7.94 -3.62 -1.99 -0.55 -2.24 -10.89 -5.52 -3.48 0.13
Bud Neck 4.00 -1.17 -3.10 -5.68 -1.76 -0.09 -2.39 -0.15 -0.08 1.84 1.10 -3.05 -2.81 -3.53 0.21
Bud Site -0.45 -10.24 -8.89 -8.72 0.80 -2.63 -8.91 -2.38 -2.28 -1.46 -1.14 -12.39 -9.35 -9.14 -3.47
Cell Periphery -1.51 -6.17 -1.82 -1.67 -0.92 6.30 0.18 2.22 -2.22 2.09 3.02 -4.60 -1.72 -1.90 -1.38
Cytoplasm 2.36 -3.03 -10.84 -11.85 -4.81 5.70 1.65 -0.68 -2.13 -1.23 5.91 -0.83 -10.71 -12.89 -9.22
Cytoplasmic Foci 17.61 29.52 48.36 28.68 2.87 6.18 50.97 13.96 11.76 -4.45 17.09 63.94 50.91 37.61 -3.89
Eisosomes 0.18 -2.57 -4.16 -4.28 -0.94 9.24 -0.45 0.98 -2.92 1.19 7.77 -2.78 -2.56 -6.36 -0.09
Endoplasmic Reticulum -3.16 -7.16 -7.01 -5.79 4.19 2.39 -9.20 -3.25 -3.85 4.15 0.30 -11.66 -7.30 -7.54 6.76
Endosome -7.65 -2.86 0.67 6.35 2.92 -9.49 4.26 -0.56 2.95 -1.92 -12.65 1.15 0.97 8.88 0.13
Golgi -3.32 14.23 6.81 8.33 -0.31 -2.35 15.82 14.91 15.63 -0.43 -3.51 22.96 7.35 9.01 -1.42
Lipid Particles -3.99 8.55 14.45 15.39 5.14 3.14 15.97 16.27 15.25 1.29 -1.08 17.91 22.48 22.88 6.12
Mitochondria -0.77 4.08 1.14 1.89 -2.09 -0.49 5.42 6.42 6.24 3.50 -1.07 6.04 2.10 2.93 -1.59
Mitotic Spindle -15.58* -11.05 -21.45 -11.96 -2.88 -15.30* -29.80* -9.15 -6.47 2.20 -22.01 -30.49 -23.30 -14.52 4.17
None 6.66 11.04 5.66 -0.50 -4.70 7.88 7.83 8.74 2.54 3.08 10.81 12.29 7.87 -1.53 -2.98
Nuclear Periphery -1.89 -4.90 -10.45 -7.05 -2.40 1.91 -6.68 -2.20 -3.25 3.31 0.52 -8.21 -8.10 -9.43 4.08
Nuclear Periphery Foci -1.43 -8.66 3.79 4.48 9.49 5.81 -7.63 -0.24 -2.02 4.41 3.70 -11.66 4.32 1.50 13.15
Nucleolus 3.66 6.17 4.35 1.25 -1.72 3.53 -0.41 10.62 8.42 7.16 5.19 1.49 5.45 1.83 2.82
Nucleus -0.88 -2.57 -9.21 -8.63 -4.34 6.96 -5.50 4.64 -0.88 7.65 4.89 -6.77 -8.11 -9.67 -0.94
Peroxisomes -7.82 6.79 10.42 15.50 0.50 -6.85 15.45 2.36 5.13 -2.76 -10.23 18.39 10.96 18.44 -3.39
Vacuole -3.33 -7.08 -11.92 -11.45 -1.19 -3.47 -8.49 -4.39 -4.25 -2.64 -4.69 -10.95 -12.71 -12.24 -4.96
Vacuole Periphery -2.81 -2.32 -1.55 -1.17 -1.34 -2.42 0.31 2.68 2.64 1.76 -2.76 -0.75 -1.53 -0.24 -1.48
Standard name
Human Ortholog
Description Centrin (Cdc31p)-binding protein required for SPB duplication; localizes to the half-bridge of the spindle pole body (SPB); required for progression through G(2)-M transition; phosphorylated by Cdc28p-Clb2p and by Cdc5p; dephosphorylated by Cdc14p; has similarity to Xenopus laevis XCAP-C
Localization
Cell Percentages spindle pole (46%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Sfi1

Sfi1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sfi1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available