ORF | |
---|---|
Human Ortholog | |
Description | Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 3 | 0 | 7 | 2 | 1 | – | 3 | 6 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 |
Bud | 0 | 1 | 1 | 1 | 4 | 6 | 8 | 10 | 9 | – | 11 | 9 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 0 | 1 | 5 | 8 | 3 | – | 0 | 5 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 8 | 3 | 16 | 15 | 30 | 72 | 103 | 91 | 92 | – | 66 | 112 | 16 | 13 | 20 | 17 | 16 | 21 | 1 | 1 | 3 | 0 | 0 | 1 |
Endoplasmic Reticulum | 1 | 0 | 1 | 1 | 6 | 4 | 27 | 13 | 11 | – | 12 | 32 | 2 | 2 | 3 | 7 | 1 | 4 | 0 | 0 | 2 | 0 | 4 | 3 |
Endosome | 1 | 0 | 2 | 9 | 53 | 46 | 59 | 38 | 24 | – | 23 | 28 | 3 | 8 | 9 | 6 | 0 | 4 | 4 | 3 | 8 | 11 | 20 | 23 |
Golgi | 0 | 2 | 0 | 0 | 0 | 1 | 7 | 2 | 2 | – | 0 | 2 | 0 | 3 | 0 | 8 | 8 | 5 | 1 | 0 | 2 | 0 | 0 | 4 |
Mitochondria | 15 | 14 | 10 | 17 | 11 | 45 | 69 | 39 | 102 | – | 113 | 94 | 15 | 4 | 15 | 7 | 3 | 3 | 1 | 1 | 8 | 2 | 4 | 5 |
Nucleus | 13 | 23 | 33 | 8 | 9 | 7 | 24 | 15 | 9 | – | 8 | 15 | 30 | 19 | 30 | 9 | 6 | 7 | 5 | 8 | 6 | 15 | 16 | 17 |
Nuclear Periphery | 25 | 10 | 13 | 5 | 2 | 4 | 11 | 3 | 7 | – | 7 | 3 | 37 | 54 | 72 | 12 | 1 | 11 | 21 | 4 | 20 | 5 | 29 | 32 |
Nucleolus | 3 | 4 | 0 | 2 | 6 | 9 | 16 | 1 | 4 | – | 9 | 4 | 3 | 5 | 6 | 3 | 2 | 4 | 1 | 0 | 0 | 2 | 3 | 3 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | – | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 15 | 11 | 19 | 17 | 8 | – | 10 | 10 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 2 |
Vac/Vac Membrane | 125 | 119 | 243 | 132 | 195 | 208 | 274 | 214 | 132 | – | 151 | 200 | 374 | 498 | 570 | 152 | 101 | 118 | 126 | 129 | 253 | 266 | 437 | 410 |
Unique Cell Count | 154 | 147 | 282 | 164 | 281 | 336 | 512 | 370 | 322 | 317 | 424 | 429 | 537 | 630 | 183 | 125 | 149 | 168 | 155 | 311 | 309 | 527 | 515 | |
Labelled Cell Count | 191 | 176 | 320 | 190 | 334 | 416 | 629 | 454 | 404 | 413 | 523 | 481 | 606 | 726 | 224 | 139 | 181 | 168 | 155 | 311 | 309 | 527 | 515 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.8 | 7.8 | 7.7 | 6.8 | 7.6 | 6.6 | 6.8 | 7.0 | 6.2 | – | 6.1 | 6.4 | 8.7 | 8.9 | 9.0 | 12.5 | 12.5 | 12.5 | 7.3 | 7.6 | 8.4 |
Std Deviation (1e-4) | 1.4 | 1.9 | 1.1 | 1.2 | 1.3 | 1.5 | 1.3 | 1.3 | 1.1 | – | 1.3 | 1.4 | 5.0 | 1.4 | 1.6 | 3.1 | 3.0 | 3.4 | 1.5 | 1.5 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.19 | -0.01 | -0.22 | -0.19 | -0.15 | -0.31 | – | -0.35 | -0.26 | 0.17 | 0.2 | 0.22 | 0.7 | 0.7 | 0.7 | -0.07 | -0.02 | 0.12 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 2.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.4 | 2.2 | 5.6 | 5.5 | 6.5 | 7.3 | – | 5.4 | 7.0 | -1.2 | -2.4 | -1.8 | 1.5 | 2.5 | 3.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 3.6 | 2.8 | 2.7 | – | 2.9 | 4.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 7.3 | 6.0 | 5.5 | 5.0 | 4.1 | – | 4.0 | 3.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.9 | 0.2 | 4.3 | 4.5 | 3.4 | 8.9 | – | 9.7 | 6.8 | 0 | 0 | -1.0 | 0.2 | 0 | 0 |
Nucleus | -2.4 | -3.8 | -4.8 | -3.7 | -3.7 | -4.3 | – | -4.4 | -4.2 | -2.2 | -4.6 | -3.8 | -2.5 | -2.2 | -2.4 |
Nuclear Periphery | -0.8 | -2.9 | -2.6 | -1.9 | -3.1 | -1.7 | – | -1.6 | -3.4 | 2.0 | 2.7 | 3.3 | 0.9 | 0 | 1.2 |
Nucleolus | 0 | 0 | 0 | 3.0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 3.9 | 3.1 | 3.3 | 3.6 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.6 | -4.8 | -6.8 | -9.2 | -7.8 | -11.4 | – | -9.9 | -10.5 | 0.4 | 3.0 | 1.9 | -0.9 | -1.4 | -1.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.8362 | 3.7443 | 3.3398 | 2.8605 | 3.2647 | 3.0245 | 2.3198 | 2.6133 | 2.2779 | 2.1973 | 1.9469 | 2.1996 | 3.7329 | 3.2058 | 3.6895 | 3.2604 | 2.9808 | 3.2226 |
Actin | 0.0369 | 0.0003 | 0.0009 | 0.0049 | 0 | 0.0012 | 0.0044 | 0 | 0.0012 | 0.001 | 0.0016 | 0.0031 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0.0001 |
Bud | 0.0005 | 0.0002 | 0.0004 | 0.0003 | 0.0001 | 0.0002 | 0.0005 | 0.0002 | 0.0011 | 0.0007 | 0.0002 | 0.0002 | 0.0003 | 0.0023 | 0.0011 | 0.0003 | 0.0003 | 0.0011 |
Bud Neck | 0.0008 | 0.0007 | 0.0003 | 0.0004 | 0.0001 | 0.0003 | 0.0012 | 0.0002 | 0.0005 | 0.0002 | 0.0003 | 0.0003 | 0.0006 | 0.0012 | 0.0013 | 0.0002 | 0.0005 | 0.0008 |
Bud Periphery | 0.0013 | 0.0006 | 0.0011 | 0.0007 | 0.0003 | 0.0007 | 0.001 | 0.0005 | 0.0009 | 0.0004 | 0.0007 | 0.0007 | 0.0011 | 0.0033 | 0.0023 | 0.0017 | 0.0009 | 0.0017 |
Bud Site | 0.0028 | 0.0011 | 0.0006 | 0.0013 | 0.0001 | 0.0002 | 0.0029 | 0.0002 | 0.0027 | 0.0005 | 0.0013 | 0.0002 | 0.0008 | 0.0042 | 0.0029 | 0.0001 | 0.0003 | 0.0005 |
Cell Periphery | 0.0022 | 0.0014 | 0.001 | 0.0005 | 0.0004 | 0.0009 | 0.0014 | 0.0005 | 0.0005 | 0.0002 | 0.0005 | 0.0004 | 0.0021 | 0.0013 | 0.0021 | 0.0051 | 0.0007 | 0.0012 |
Cytoplasm | 0.0056 | 0.0058 | 0.0045 | 0.003 | 0.0037 | 0.0012 | 0.0059 | 0.0092 | 0.0022 | 0.0039 | 0.0007 | 0.0014 | 0.0028 | 0.0084 | 0.0095 | 0.0006 | 0.0042 | 0.0059 |
Cytoplasmic Foci | 0.0103 | 0.0054 | 0.0064 | 0.0125 | 0.0065 | 0.0011 | 0.0219 | 0.0013 | 0.0078 | 0.05 | 0.002 | 0.0044 | 0.0061 | 0.0044 | 0.014 | 0.0012 | 0.0231 | 0.0112 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0.0006 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0054 | 0.0073 | 0.0035 | 0.0023 | 0.0027 | 0.0059 | 0.0037 | 0.0042 | 0.0015 | 0.0006 | 0.0016 | 0.0018 | 0.0018 | 0.0041 | 0.0033 | 0.0009 | 0.0016 | 0.002 |
Endosome | 0.1247 | 0.1105 | 0.1511 | 0.1506 | 0.2301 | 0.1123 | 0.1728 | 0.0912 | 0.131 | 0.1376 | 0.0973 | 0.1134 | 0.1114 | 0.1305 | 0.1611 | 0.1644 | 0.2032 | 0.1123 |
Golgi | 0.0136 | 0.0025 | 0.0057 | 0.0202 | 0.0029 | 0.0101 | 0.0128 | 0.0011 | 0.0115 | 0.007 | 0.0043 | 0.005 | 0.003 | 0.0027 | 0.004 | 0.0025 | 0.0046 | 0.0026 |
Lipid Particles | 0.0115 | 0.0055 | 0.0215 | 0.0343 | 0.0063 | 0.0058 | 0.0121 | 0.0007 | 0.0036 | 0.0045 | 0.0137 | 0.007 | 0.0147 | 0.0033 | 0.0155 | 0.0186 | 0.0047 | 0.0174 |
Mitochondria | 0.0324 | 0.0092 | 0.0454 | 0.0184 | 0.0051 | 0.0417 | 0.0174 | 0.0081 | 0.0347 | 0.0263 | 0.0424 | 0.0152 | 0.0598 | 0.0049 | 0.0462 | 0.0191 | 0.0243 | 0.0174 |
None | 0.0162 | 0.0075 | 0.037 | 0.0865 | 0.0004 | 0.0003 | 0.0012 | 0.0004 | 0.0004 | 0.0004 | 0.001 | 0.0006 | 0.0046 | 0.0091 | 0.0222 | 0.0001 | 0.0012 | 0.0094 |
Nuclear Periphery | 0.0198 | 0.0244 | 0.0133 | 0.0076 | 0.0076 | 0.0285 | 0.0175 | 0.0167 | 0.0169 | 0.0028 | 0.0085 | 0.0158 | 0.0064 | 0.0138 | 0.012 | 0.0045 | 0.0054 | 0.0364 |
Nucleolus | 0.004 | 0.0032 | 0.0025 | 0.0018 | 0.0003 | 0.0006 | 0.0025 | 0.0005 | 0.0019 | 0.0023 | 0.0019 | 0.0008 | 0.0183 | 0.0264 | 0.0164 | 0.0015 | 0.0041 | 0.0183 |
Nucleus | 0.0074 | 0.0046 | 0.0102 | 0.0016 | 0.0009 | 0.0028 | 0.0029 | 0.0022 | 0.0065 | 0.0011 | 0.0012 | 0.0019 | 0.0387 | 0.0197 | 0.0215 | 0.0017 | 0.0039 | 0.0187 |
Peroxisomes | 0.019 | 0.0004 | 0.005 | 0.0067 | 0.0002 | 0.0004 | 0.0029 | 0.0001 | 0.0014 | 0.0015 | 0.0052 | 0.0004 | 0.0032 | 0.0005 | 0.0015 | 0.0011 | 0.0012 | 0.0016 |
Punctate Nuclear | 0.0034 | 0.0003 | 0.0009 | 0.001 | 0.0001 | 0 | 0.0017 | 0 | 0.0001 | 0.0077 | 0.001 | 0.0004 | 0.0004 | 0.0008 | 0.0026 | 0 | 0.0001 | 0.0029 |
Vacuole | 0.4465 | 0.479 | 0.3788 | 0.4095 | 0.3933 | 0.3552 | 0.4275 | 0.4721 | 0.4035 | 0.3948 | 0.39 | 0.3478 | 0.4237 | 0.4602 | 0.389 | 0.3922 | 0.4178 | 0.341 |
Vacuole Periphery | 0.2355 | 0.3303 | 0.3099 | 0.2352 | 0.3391 | 0.4305 | 0.2856 | 0.3907 | 0.3701 | 0.3563 | 0.4241 | 0.4791 | 0.2999 | 0.2988 | 0.2711 | 0.3844 | 0.2979 | 0.3975 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 6.1797 | 5.3338 | 7.0677 | 12.7695 | 7.1835 | 8.5191 | 10.4008 | 10.3998 | 13.7751 | 12.7793 |
Translational Efficiency | 0.8241 | 0.6689 | 0.8946 | 0.463 | 0.6662 | 0.7291 | 0.6493 | 0.4944 | 0.5695 | 0.6087 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1748 | 1504 | 139 | 1213 | 1501 | 1925 | 169 | 102 | 3249 | 3429 | 308 | 1315 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 756.03 | 943.60 | 1463.79 | 956.25 | 897.69 | 965.22 | 1165.74 | 1190.89 | 821.48 | 955.74 | 1300.25 | 974.45 |
Standard Deviation | 102.16 | 159.18 | 184.96 | 136.69 | 127.70 | 149.96 | 133.62 | 235.15 | 134.67 | 154.45 | 217.33 | 159.57 |
Intensity Change Log 2 | — | 0.319732 | 0.953193 | 0.338944 | — | 0.104640 | 0.376957 | 0.407751 | — | 0.206966 | 0.669090 | 0.376701 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000015 | 0.000025 | 0.000305 | 0.000256 | 0.000044 | 0.000066 | 0.000175 | 0.000026 | 0.000028 | 0.000048 | 0.000234 | 0.000238 |
Bud Neck | 0.002077 | 0.000882 | 0.015243 | 0.007362 | 0.000629 | 0.000549 | 0.020376 | 0.003106 | 0.001408 | 0.000695 | 0.018060 | 0.007032 |
Bud Site | 0.009768 | 0.004619 | 0.004108 | 0.018183 | 0.003108 | 0.002511 | 0.009036 | 0.007660 | 0.006691 | 0.003436 | 0.006812 | 0.017367 |
Cell Periphery | 0.000223 | 0.000194 | 0.000085 | 0.000131 | 0.000102 | 0.000176 | 0.000104 | 0.000048 | 0.000167 | 0.000184 | 0.000095 | 0.000124 |
Cytoplasm | 0.021031 | 0.008515 | 0.000393 | 0.013449 | 0.006749 | 0.001789 | 0.002263 | 0.011816 | 0.014433 | 0.004739 | 0.001419 | 0.013322 |
Cytoplasmic Foci | 0.012278 | 0.003474 | 0.002367 | 0.009270 | 0.005874 | 0.003559 | 0.001913 | 0.011289 | 0.009320 | 0.003522 | 0.002118 | 0.009427 |
Eisosomes | 0.000008 | 0.000005 | 0.000032 | 0.000014 | 0.000004 | 0.000005 | 0.000044 | 0.000004 | 0.000006 | 0.000005 | 0.000039 | 0.000013 |
Endoplasmic Reticulum | 0.002568 | 0.001550 | 0.001678 | 0.003211 | 0.001702 | 0.001365 | 0.003763 | 0.000744 | 0.002168 | 0.001446 | 0.002822 | 0.003020 |
Endosome | 0.039603 | 0.013830 | 0.008060 | 0.053471 | 0.019057 | 0.013349 | 0.031456 | 0.024074 | 0.030111 | 0.013560 | 0.020898 | 0.051190 |
Golgi | 0.004457 | 0.001928 | 0.003686 | 0.006569 | 0.002153 | 0.003071 | 0.001972 | 0.004712 | 0.003393 | 0.002569 | 0.002746 | 0.006425 |
Lipid Particles | 0.000587 | 0.000490 | 0.000479 | 0.001153 | 0.000390 | 0.000764 | 0.000951 | 0.000359 | 0.000496 | 0.000644 | 0.000738 | 0.001092 |
Mitochondria | 0.013588 | 0.003093 | 0.003404 | 0.005794 | 0.003058 | 0.007836 | 0.002190 | 0.000192 | 0.008723 | 0.005756 | 0.002738 | 0.005359 |
Mitotic Spindle | 0.000282 | 0.000325 | 0.008345 | 0.008401 | 0.000154 | 0.000155 | 0.001209 | 0.001262 | 0.000223 | 0.000229 | 0.004429 | 0.007847 |
None | 0.000848 | 0.000259 | 0.000168 | 0.000710 | 0.000130 | 0.000101 | 0.000217 | 0.000042 | 0.000517 | 0.000170 | 0.000195 | 0.000658 |
Nuclear Periphery | 0.000198 | 0.001362 | 0.025652 | 0.002854 | 0.000574 | 0.000715 | 0.005317 | 0.000704 | 0.000372 | 0.000999 | 0.014494 | 0.002687 |
Nuclear Periphery Foci | 0.000098 | 0.000337 | 0.009944 | 0.003247 | 0.000288 | 0.000260 | 0.002684 | 0.001295 | 0.000186 | 0.000294 | 0.005961 | 0.003096 |
Nucleolus | 0.000471 | 0.000691 | 0.048506 | 0.004706 | 0.000573 | 0.000546 | 0.047160 | 0.000920 | 0.000518 | 0.000610 | 0.047768 | 0.004413 |
Nucleus | 0.002664 | 0.002060 | 0.036716 | 0.011276 | 0.002466 | 0.000944 | 0.068223 | 0.001968 | 0.002573 | 0.001433 | 0.054004 | 0.010554 |
Peroxisomes | 0.000409 | 0.000287 | 0.002043 | 0.000887 | 0.000224 | 0.000452 | 0.002459 | 0.000416 | 0.000324 | 0.000379 | 0.002271 | 0.000851 |
Vacuole | 0.371727* | 0.324642* | 0.294484* | 0.698921* | 0.371949* | 0.320813* | 0.530149* | 0.695510* | 0.371829* | 0.322493* | 0.423794* | 0.698656* |
Vacuole Periphery | 0.517098* | 0.631432* | 0.534303* | 0.150135* | 0.580771* | 0.640974* | 0.268338* | 0.233854* | 0.546514* | 0.636789* | 0.388367* | 0.156629* |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.00 | -3.66 | -3.65 | -3.46 | 0.95 | -0.59 | -3.29 | 0.93 | 1.15 | 3.93 | -0.99 | -4.85 | -3.41 | -2.95 | 0.39 |
Bud Neck | 3.73 | -5.01 | -8.35 | -10.47 | 2.83 | 0.21 | -6.84 | -3.52 | -3.63 | 5.76 | 3.40 | -8.40 | -9.78 | -11.05 | 5.05 |
Bud Site | 4.24 | 3.51 | -3.57 | -5.79 | -5.47 | 0.72 | -2.92 | -1.49 | -1.65 | 0.40 | 4.53 | -0.45 | -5.06 | -6.60 | -4.22 |
Cell Periphery | 0.67 | 5.31 | 3.41 | 1.71 | -2.30 | -3.89 | -0.11 | 3.81 | 6.88 | 2.83 | -0.75 | 4.08 | 2.35 | 2.76 | -1.44 |
Cytoplasm | 4.87 | 10.48 | 1.92 | -3.32 | -9.92 | 3.72 | 2.30 | -1.89 | -3.69 | -3.17 | 6.75 | 9.08 | -0.79 | -6.68 | -8.67 |
Cytoplasmic Foci | 8.93 | 8.76 | 2.28 | -5.15 | -5.46 | 2.03 | 3.94 | -1.69 | -2.45 | -3.01 | 7.63 | 9.69 | -0.10 | -5.65 | -7.05 |
Eisosomes | 3.81 | -4.28 | -4.96 | -7.85 | 3.27 | -1.32 | -7.90 | -0.70 | 0.51 | 7.71 | 1.80 | -8.53 | -7.09 | -7.85 | 6.52 |
Endoplasmic Reticulum | 2.64 | 1.33 | -1.26 | -4.01 | -2.38 | 1.65 | -3.61 | 3.25 | 2.22 | 4.99 | 3.23 | -1.83 | -2.16 | -4.44 | -0.12 |
Endosome | 10.72 | 11.51 | -3.91 | -13.44 | -14.07 | 3.04 | -3.01 | -1.42 | -3.07 | 1.36 | 10.65 | 3.30 | -7.32 | -14.46 | -8.67 |
Golgi | 4.37 | 0.62 | -1.86 | -4.38 | -1.85 | -1.15 | 0.33 | -1.73 | -1.03 | -1.93 | 1.60 | 1.04 | -3.08 | -3.83 | -3.45 |
Lipid Particles | 0.34 | 0.23 | -1.70 | -1.69 | -1.80 | -1.25 | -2.40 | -0.11 | 1.18 | 2.28 | -0.79 | -1.86 | -1.99 | -1.24 | -0.99 |
Mitochondria | 7.27 | 7.02 | 4.39 | -2.09 | -1.81 | -3.52 | 1.17 | 4.86 | 6.25 | 4.17 | 2.78 | 6.95 | 2.60 | 0.39 | -2.24 |
Mitotic Spindle | -0.48 | -1.91 | -5.20 | -5.16 | 0.05 | -0.07 | -2.37 | -2.12 | -2.11 | -0.11 | -0.22 | -2.17 | -5.29 | -5.28 | -1.33 |
None | 4.22 | 5.28 | 0.50 | -1.85 | -2.27 | 1.08 | -1.71 | 3.09 | 2.98 | 3.74 | 4.66 | 4.35 | -0.63 | -2.21 | -2.11 |
Nuclear Periphery | -2.61 | -3.73 | -5.01 | -2.27 | 3.31 | -0.42 | -2.70 | -0.78 | -0.24 | 2.53 | -2.33 | -4.31 | -4.73 | -3.14 | 3.53 |
Nuclear Periphery Foci | -3.40 | -3.54 | -3.56 | -3.27 | 2.34 | 0.14 | -1.89 | -2.00 | -2.03 | 0.95 | -1.47 | -3.99 | -3.58 | -3.43 | 1.84 |
Nucleolus | -1.66 | -5.24 | -6.95 | -6.52 | 4.75 | 0.01 | -6.77 | -1.72 | -1.54 | 6.71 | -0.83 | -8.42 | -6.92 | -6.67 | 7.64 |
Nucleus | 0.90 | -3.48 | -6.60 | -7.29 | 2.54 | 2.01 | -6.79 | 0.46 | -2.06 | 6.84 | 2.30 | -7.35 | -6.74 | -7.89 | 6.00 |
Peroxisomes | 1.00 | -2.92 | -2.22 | -2.83 | 2.14 | -0.91 | -3.68 | -1.83 | 0.27 | 3.34 | -0.49 | -4.65 | -2.79 | -1.79 | 3.24 |
Vacuole | 5.82 | 4.28 | -36.83 | -41.18 | -22.28 | 6.43 | -8.61 | -14.87 | -17.38 | -6.13 | 8.75 | -3.70 | -42.11 | -49.24 | -17.80 |
Vacuole Periphery | -11.77 | -0.19 | 44.53* | 58.14* | 14.47 | -6.70 | 16.43 | 15.84 | 18.78 | 1.38 | -13.67 | 9.44 | 57.98 | 74.69* | 13.16 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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