Standard name
Human Ortholog
Description Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.05 0 0 0 0 0 0
Bud 0 0 0.1 0.09 0.09 0.07 0.08 0.07 0.07 0.09 0.06 0.1 0 0 0 0 0.07 0 0 0 0 0 0 0
Bud Neck 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0 0 0 0 0 0 0
Cytoplasm 0.74 0.74 0.76 0.71 0.74 0.63 0.59 0.62 0.57 0.61 0.49 0.51 0.89 0.91 0.9 0.46 0.37 0.56 0.54 0.6 0.42 0.83 0.89 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0 0.06 0 0 0 0 0 0
Endosome 0 0.1 0 0 0.06 0 0 0 0 0 0 0 0.09 0.06 0 0.23 0.07 0.16 0.08 0.08 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.08 0.07 0.2 0.06 0.25 0.27 0.35 0.43 0.43 0.45 0.48 0 0 0.05 0.11 0 0.05 0 0.07 0.15 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.19 0.08 0.14 0.16 0.13 0.16 0.12 0.13 0.13 0.1 0.14 0.13 0 0 0 0.23 0.41 0.34 0.23 0.14 0.24 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0.06 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 4 3 3 1 4 1 3 1 0 5 0 1 4 1 1 3 3 5 7 7 2 1 3
Bud 2 7 19 17 18 27 21 25 21 30 14 30 3 1 9 1 2 2 1 1 2 1 1 2
Bud Neck 1 0 11 5 6 11 8 4 5 6 3 1 0 0 0 1 1 0 2 5 3 2 0 1
Bud Site 1 0 0 4 2 2 4 8 10 6 5 6 0 0 0 0 1 0
Cell Periphery 0 1 2 1 2 2 0 2 1 0 5 5 0 1 1 0 3 2 0 0 1 0 0 0
Cytoplasm 102 180 140 139 151 227 161 234 162 206 110 152 151 170 180 16 10 35 87 166 100 114 149 158
Endoplasmic Reticulum 5 8 1 1 1 3 0 1 0 1 3 0 0 3 1 4 1 4 2 2 1 0 0 3
Endosome 6 23 0 0 12 13 9 5 5 0 2 2 16 11 7 8 2 10 12 22 9 1 1 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 2
Mitochondria 0 20 13 39 13 91 74 131 123 143 101 145 0 0 10 4 1 3 7 18 36 4 4 3
Nucleus 0 1 0 0 0 1 1 3 2 2 5 0 1 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Nucleolus 1 0 0 0 1 0 1 1 1 0 2 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 7 0 0 2 5 3 1 5 2 6 5 1 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 26 19 26 31 27 57 34 48 37 35 32 40 5 2 1 8 11 21 37 40 57 4 0 0
Vac/Vac Membrane 0 0 0 0 0 0 1 3 2 0 1 3 0 0 1 3 1 0 1 3 13 3 3 5
Unique Cell Count 138 242 185 197 205 361 272 375 283 335 223 299 170 187 201 35 27 62 161 279 240 137 169 189
Labelled Cell Count 149 270 215 240 236 443 319 470 375 431 294 389 178 193 211 47 36 81 161 279 240 137 169 189


Ambiguous, SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 4.5 5.4 3.6 4.1 3.6 3.9 3.6 3.1 3.1 3.3 2.9 5.0 5.2 4.6 6.4 6.8 7.5 5.0 5.2 6.0
Std Deviation (1e-4) 0.6 1.0 5.6 0.9 1.6 1.1 2.3 1.1 1.1 1.1 1.6 0.7 0.9 1.4 1.2 1.2 0.7 2.0 1.2 1.3 1.6
Intensity Change (Log2) -0.61 -0.4 -0.59 -0.48 -0.59 -0.8 -0.83 -0.7 -0.92 -0.11 -0.06 -0.24 0.25 0.33 0.46 -0.11 -0.06 0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.6475 1.5231 1.2555 1.0154 1.2037 1.3745 1.9578 2.4268 2.3248 2.3745 2.2888 2.2438 2.8053 3.9317 3.1538 2.3278 2.6279 3.2525
Actin 0.076 0.0131 0.0166 0.0183 0.0267 0.0067 0.0111 0.0023 0.0105 0.0205 0.0073 0.0065 0.0063 0.0163 0.0058 0.009 0.0029 0.006
Bud 0.0017 0.0023 0.002 0.0017 0.0007 0.0003 0.0004 0.003 0.0023 0.0006 0.0015 0.0001 0.0007 0.0003 0.0002 0.0005 0.0005 0.0001
Bud Neck 0.0016 0.0025 0.0038 0.0159 0.0044 0.0055 0.003 0.0003 0.001 0.0226 0.0009 0.0009 0.0007 0.0003 0.0019 0.0351 0.0004 0.0016
Bud Periphery 0.0035 0.0008 0.0028 0.0011 0.0012 0.0006 0.0006 0.0083 0.0067 0.0019 0.0044 0.0001 0.0023 0.0003 0.0003 0.0004 0.0005 0.0001
Bud Site 0.0058 0.0149 0.0125 0.007 0.004 0.0008 0.0061 0.0053 0.0094 0.0022 0.003 0.0004 0.0014 0.0027 0.0009 0.0021 0.0006 0.0002
Cell Periphery 0.0012 0.0008 0.0005 0.0004 0.0003 0.0002 0.0006 0.0003 0.0003 0.0015 0.0003 0.0001 0.0012 0.0006 0.0002 0.0001 0.0001 0.0001
Cytoplasm 0.3555 0.4785 0.4667 0.5246 0.1028 0.3786 0.3128 0.4481 0.3029 0.3714 0.1733 0.3779 0.3549 0.4349 0.3851 0.3703 0.07 0.3065
Cytoplasmic Foci 0.0964 0.1442 0.1359 0.0811 0.4912 0.247 0.1641 0.119 0.1711 0.0584 0.2601 0.2331 0.2757 0.1511 0.1738 0.2553 0.5389 0.2578
Eisosomes 0.0006 0.0006 0.0002 0.0004 0.0003 0.0001 0.0005 0.0001 0.0003 0.0003 0.0002 0 0.0002 0.0002 0.0002 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0139 0.0049 0.0108 0.007 0.0019 0.0049 0.0058 0.0021 0.003 0.009 0.0053 0.0022 0.0098 0.0092 0.009 0.006 0.0045 0.0037
Endosome 0.047 0.0128 0.0423 0.0587 0.0759 0.0428 0.0524 0.0146 0.0385 0.0299 0.117 0.032 0.0293 0.0654 0.0421 0.0426 0.0636 0.0323
Golgi 0.0143 0.002 0.0041 0.0085 0.0182 0.0037 0.0096 0.001 0.0114 0.0037 0.0114 0.0032 0.0032 0.0039 0.0032 0.0071 0.0085 0.0029
Lipid Particles 0.02 0.0042 0.0097 0.0158 0.0901 0.0068 0.0313 0.0008 0.009 0.0089 0.0254 0.0028 0.0078 0.0146 0.0047 0.0035 0.0175 0.0138
Mitochondria 0.0104 0.0025 0.0031 0.0293 0.0053 0.0032 0.0049 0.0012 0.0068 0.0019 0.0622 0.0006 0.0006 0.0003 0.0007 0.0012 0.0011 0.0006
None 0.2869 0.2977 0.2495 0.192 0.1331 0.188 0.3264 0.3754 0.3918 0.4397 0.2718 0.253 0.2755 0.288 0.3557 0.1038 0.1334 0.2611
Nuclear Periphery 0.0147 0.0009 0.0067 0.0019 0.0018 0.0012 0.0107 0.0004 0.0016 0.0085 0.0072 0.0005 0.0025 0.0019 0.0013 0.0521 0.0428 0.0008
Nucleolus 0.0006 0.0003 0.0012 0.0021 0.0007 0.0004 0.0066 0.0002 0.0003 0.0005 0.0012 0.0001 0.0007 0.0002 0.0002 0.0008 0.0013 0.0004
Nucleus 0.0163 0.0013 0.0055 0.002 0.0011 0.0013 0.007 0.0009 0.0012 0.0039 0.0079 0.0007 0.0028 0.0011 0.0011 0.0689 0.0274 0.001
Peroxisomes 0.0105 0.0027 0.0078 0.0091 0.0313 0.0044 0.0226 0.0028 0.0182 0.002 0.0193 0.0083 0.0099 0.0019 0.0086 0.0159 0.0557 0.014
Punctate Nuclear 0.0167 0.0108 0.011 0.0019 0.006 0.0995 0.0172 0.0122 0.0091 0.0086 0.0083 0.0766 0.0125 0.0022 0.0035 0.0239 0.0281 0.0953
Vacuole 0.0045 0.0018 0.0062 0.0189 0.002 0.003 0.0051 0.0014 0.0038 0.0033 0.0088 0.0009 0.0018 0.0042 0.0016 0.0008 0.0016 0.0013
Vacuole Periphery 0.0016 0.0002 0.0011 0.0024 0.0007 0.0009 0.0012 0.0002 0.001 0.0007 0.0033 0.0002 0.0003 0.0004 0.0002 0.0006 0.0006 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.6016 6.07 5.7554 7.4391 4.891 6.4094 8.5241 8.7449 6.8471 8.3832
Translational Efficiency 0.529 0.451 0.4032 0.2901 0.5795 0.5779 0.3958 0.3308 0.4755 0.4456

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1897 100 1884 796 1496 1195 151 83 3393 1295 2035 879

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 657.45 768.24 1023.17 896.73 697.28 763.28 996.26 956.39 675.01 763.66 1021.17 902.36
Standard Deviation 84.21 92.20 123.01 116.49 80.99 98.61 107.05 120.76 85.13 98.14 122.11 118.20
Intensity Change Log 2 0.224676 0.638093 0.447792 0.130474 0.514784 0.455861 0.176959 0.575943 0.451951

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000144 0.001613 0.000532 0.005217 0.000190 0.000430 0.000513 0.000822 0.000164 0.000522 0.000530 0.004802
Bud Neck 0.015849 0.014044 0.002342 0.006019 0.013116 0.017100 0.002485 0.007725 0.014644 0.016864 0.002353 0.006180
Bud Site 0.004435 0.015850 0.020332 0.078450 0.004130 0.014538 0.006069 0.044181 0.004301 0.014639 0.019273 0.075214
Cell Periphery 0.000102 0.000091 0.000058 0.000186 0.000116 0.000112 0.000165 0.000157 0.000108 0.000111 0.000066 0.000183
Cytoplasm 0.290792* 0.208738* 0.749391* 0.239838* 0.294866* 0.313854* 0.306045* 0.140697* 0.292588* 0.305737* 0.716494* 0.230476*
Cytoplasmic Foci 0.483288* 0.441110* 0.077074 0.119992 0.490197* 0.408514* 0.060298 0.060047 0.486334* 0.411031* 0.075829 0.114332
Eisosomes 0.000235 0.000195 0.000010 0.000053 0.000207 0.000151 0.000119 0.000089 0.000223 0.000155 0.000018 0.000056
Endoplasmic Reticulum 0.000693 0.000599 0.000603 0.001156 0.000954 0.000514 0.004417 0.003309 0.000808 0.000521 0.000886 0.001359
Endosome 0.049445 0.087487 0.005693 0.030256 0.037936 0.060233 0.033436 0.032599 0.044371 0.062337 0.007751 0.030477
Golgi 0.006846 0.007125 0.000698 0.011095 0.010745 0.009028 0.000320 0.002521 0.008565 0.008881 0.000670 0.010285
Lipid Particles 0.028799 0.010995 0.001231 0.007865 0.033659 0.013594 0.007546 0.008309 0.030942 0.013393 0.001699 0.007907
Mitochondria 0.004598 0.001336 0.000150 0.014692 0.006206 0.005081 0.000172 0.002753 0.005307 0.004792 0.000151 0.013565
Mitotic Spindle 0.004705 0.010429 0.023585 0.261309* 0.004900 0.013654 0.058360 0.258796* 0.004791 0.013405 0.026166 0.261071*
None 0.004769 0.000480 0.004989 0.009474 0.003343 0.002377 0.002119 0.000549 0.004140 0.002230 0.004776 0.008631
Nuclear Periphery 0.000315 0.000209 0.000272 0.000567 0.000369 0.000371 0.002103 0.001389 0.000339 0.000359 0.000408 0.000645
Nuclear Periphery Foci 0.001295 0.001695 0.007416 0.006712 0.001426 0.000911 0.017511 0.023118 0.001353 0.000972 0.008165 0.008261
Nucleolus 0.002934 0.001191 0.000403 0.000632 0.001988 0.002340 0.001104 0.000663 0.002517 0.002251 0.000455 0.000635
Nucleus 0.041114 0.015667 0.060864 0.015127 0.040563 0.037077 0.119538 0.073347 0.040871 0.035424 0.065217 0.020625
Peroxisomes 0.024883 0.074206 0.003564 0.052274 0.021416 0.028592 0.003550 0.012565 0.023354 0.032114 0.003563 0.048525
Vacuole 0.033582 0.105618 0.040508 0.125011 0.032382 0.069695 0.373851* 0.272430* 0.033053 0.072469 0.065243 0.138931
Vacuole Periphery 0.001178 0.001323 0.000287 0.014077 0.001290 0.001833 0.000280 0.053935 0.001227 0.001793 0.000286 0.017841

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.41 -6.75 -5.72 -1.01 -4.15 -2.06 -5.68 -2.71 -2.17 -0.99 -3.09 -7.29 -5.98 -4.68 -4.27
Bud Neck 0.69 11.43 9.22 2.96 -4.18 -2.47 4.77 2.14 3.88 -1.15 -1.96 13.54 10.29 9.05 -4.38
Bud Site -2.94 -9.15 -10.58 -5.31 -5.45 -5.26 -4.46 -3.72 -2.57 -2.65 -5.73 -9.69 -11.24 -7.35 -5.87
Cell Periphery 0.45 3.91 -0.46 -0.85 -4.74 0.64 -4.57 -1.41 -1.79 2.20 -0.35 5.26 0.03 0.34 -4.34
Cytoplasm 2.20 -42.93 7.44 0.90 43.26* -1.85 4.94 9.34 10.19 2.81 -2.42 -42.45 10.03 10.36 42.12
Cytoplasmic Foci 3.05 50.18 48.31 13.52 1.97 8.13 38.36 21.14 16.27 -2.34 10.09 62.83 57.86 35.06 1.39
Eisosomes 0.62 7.03 5.09 3.57 -18.37 4.74 2.77 4.85 1.39 1.96 3.28 11.20 8.13 8.94 -14.91
Endoplasmic Reticulum -0.02 -0.57 -5.12 -4.01 -5.88 3.08 -7.56 -4.58 -5.35 2.86 2.68 -3.24 -6.00 -8.98 -3.09
Endosome -2.38 19.24 10.87 4.55 -8.00 -6.32 0.86 1.76 5.30 0.61 -5.38 21.90 10.97 12.33 -7.38
Golgi 0.21 11.95 4.70 1.95 -2.58 1.62 13.58 12.45 8.91 -1.10 -0.16 17.74 7.63 5.94 -2.61
Lipid Particles 6.33 14.96 11.56 2.22 -3.96 8.33 10.56 9.89 2.92 0.69 9.79 20.75 15.83 5.45 -3.89
Mitochondria 3.66 5.81 1.05 -1.32 -2.32 0.96 7.43 6.77 5.20 -0.64 0.40 9.35 2.09 1.60 -2.33
Mitotic Spindle -1.73 -9.92 -21.83 -19.13 -19.22 -3.94 -4.99 -4.46 -4.11 -0.73 -4.63 -11.00 -22.10 -20.95 -18.73
None 7.28 1.17 1.36 -2.36 0.82 1.06 1.37 1.93 0.81 0.76 2.67 0.45 1.07 -0.70 0.90
Nuclear Periphery 1.17 -2.97 -13.20 -13.32 -12.20 -0.23 -6.27 -6.35 -6.18 2.39 -0.64 -6.08 -14.39 -12.57 -10.42
Nuclear Periphery Foci -0.93 -10.62 -8.74 -8.25 -3.75 1.36 -5.83 -4.03 -4.11 0.13 1.44 -11.79 -9.54 -9.74 -3.58
Nucleolus 6.57 8.45 7.23 0.05 -1.57 -1.11 -1.37 1.35 1.97 1.70 0.92 7.20 7.55 3.58 0.02
Nucleus 4.99 -9.27 4.27 -2.03 12.29 1.19 -4.99 -1.62 -1.89 2.66 1.50 -10.75 3.55 1.72 11.53
Peroxisomes -3.94 16.04 0.51 3.98 -7.04 -3.72 5.73 1.06 2.61 -1.66 -4.13 19.22 0.09 2.81 -7.25
Vacuole -4.97 -11.75 -21.97 -7.31 -18.39 -10.04 -14.59 -11.69 -10.50 -0.35 -11.65 -15.76 -24.29 -19.12 -17.46
Vacuole Periphery -0.42 4.30 -1.56 -1.40 -2.35 -3.01 9.91 -0.82 -0.74 -0.99 -3.46 6.82 -1.71 -1.20 -2.53