Standard name
Description Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.05 0 0 0 0 0 0
Bud 0 0 0.10 0.09 0.09 0.07 0.08 0.07 0.07 0.09 0.06 0.10 0 0 0 0 0.07 0 0 0 0 0 0 0
Bud Neck 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0 0 0 0 0 0 0
Cytoplasm 0.74 0.74 0.76 0.71 0.74 0.63 0.59 0.62 0.57 0.61 0.49 0.51 0.89 0.91 0.90 0.46 0.37 0.56 0.54 0.60 0.42 0.83 0.89 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0 0.06 0 0 0 0 0 0
Endosome 0 0.10 0 0 0.06 0 0 0 0 0 0 0 0.09 0.06 0 0.23 0.07 0.16 0.08 0.08 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.08 0.07 0.20 0.06 0.25 0.27 0.35 0.43 0.43 0.45 0.48 0 0 0.05 0.11 0 0.05 0 0.07 0.15 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.19 0.08 0.14 0.16 0.13 0.16 0.12 0.13 0.13 0.10 0.14 0.13 0 0 0 0.23 0.41 0.34 0.23 0.14 0.24 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0.06 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 4 3 3 1 4 1 3 1 0 5 0 1 4 1 1 3 3 5 7 7 2 1 3
Bud 2 7 19 17 18 27 21 25 21 30 14 30 3 1 9 1 2 2 1 1 2 1 1 2
Bud Neck 1 0 11 5 6 11 8 4 5 6 3 1 0 0 0 1 1 0 2 5 3 2 0 1
Bud Site 1 0 0 4 2 2 4 8 10 6 5 6 0 0 0 0 1 0
Cell Periphery 0 1 2 1 2 2 0 2 1 0 5 5 0 1 1 0 3 2 0 0 1 0 0 0
Cytoplasm 102 180 140 139 151 227 161 234 162 206 110 152 151 170 180 16 10 35 87 166 100 114 149 158
Endoplasmic Reticulum 5 8 1 1 1 3 0 1 0 1 3 0 0 3 1 4 1 4 2 2 1 0 0 3
Endosome 6 23 0 0 12 13 9 5 5 0 2 2 16 11 7 8 2 10 12 22 9 1 1 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 2
Mitochondria 0 20 13 39 13 91 74 131 123 143 101 145 0 0 10 4 1 3 7 18 36 4 4 3
Nucleus 0 1 0 0 0 1 1 3 2 2 5 0 1 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Nucleolus 1 0 0 0 1 0 1 1 1 0 2 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 7 0 0 2 5 3 1 5 2 6 5 1 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 26 19 26 31 27 57 34 48 37 35 32 40 5 2 1 8 11 21 37 40 57 4 0 0
Vac/Vac Membrane 0 0 0 0 0 0 1 3 2 0 1 3 0 0 1 3 1 0 1 3 13 3 3 5
Unique Cell Count 138 242 185 197 205 361 272 375 283 335 223 299 170 187 201 35 27 62 161 279 240 137 169 189
Labelled Cell Count 149 270 215 240 236 443 319 470 375 431 294 389 178 193 211 47 36 81 161 279 240 137 169 189

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Ambiguous, SpindlePole

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 4.5 5.4 3.6 4.1 3.6 3.9 3.6 3.1 3.1 3.3 2.9 5.0 5.2 4.6 6.4 6.8 7.5 5.0 5.2 6.0
Std Deviation (1e-4) 0.6 1.0 5.6 0.9 1.6 1.1 2.3 1.1 1.1 1.1 1.6 0.7 0.9 1.4 1.2 1.2 0.7 2.0 1.2 1.3 1.6
Intensity Change (Log2) -0.61 -0.4 -0.59 -0.48 -0.59 -0.8 -0.83 -0.7 -0.92 -0.11 -0.06 -0.24 0.25 0.33 0.46 -0.11 -0.06 0.14

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole