Standard name | |
---|---|
Human Ortholog | |
Description | DNA helicase/Ubiquitin ligase; involved in error-free DNA damage tolerance (DDT), replication fork regression during postreplication repair by template switching, error-prone translesion synthesis; promotes synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; forms nuclear foci upon DNA replication stress; associates with native telomeres, cooperates with homologous recombination in senescent cells; human homolog HLTF can complement yeast null mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud | 1 | 0 | 2 | 1 | 1 | 4 | 4 | 9 | 3 | 7 | 8 | 2 | 0 | 1 | 6 | 0 | 0 | 0 | 7 | 4 | 1 | 7 | 6 | 7 |
Bud Neck | 0 | 0 | 1 | 0 | 1 | 0 | 3 | 1 | 1 | 0 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 3 |
Bud Site | 0 | 0 | 1 | 1 | 0 | 2 | 4 | 3 | 0 | 1 | 3 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 192 | 37 | 94 | 70 | 123 | 222 | 323 | 354 | 151 | 269 | 206 | 250 | 73 | 84 | 118 | 58 | 43 | 59 | 131 | 27 | 59 | 26 | 74 | 147 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 7 | 5 | 17 | 3 | 0 | 2 | 1 | 0 | 1 |
Endosome | 17 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 5 | 1 | 4 | 7 | 8 | 3 | 1 | 1 | 2 | 0 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 0 | 1 |
Mitochondria | 21 | 56 | 59 | 77 | 100 | 136 | 145 | 189 | 96 | 198 | 170 | 187 | 18 | 13 | 64 | 8 | 5 | 15 | 37 | 4 | 15 | 1 | 6 | 12 |
Nucleus | 85 | 10 | 12 | 47 | 57 | 24 | 23 | 17 | 4 | 16 | 13 | 16 | 70 | 54 | 18 | 80 | 48 | 106 | 151 | 35 | 31 | 45 | 44 | 33 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 2 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 3 | 2 | 1 | 0 | 4 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 0 | 1 | 2 | 1 | 3 | 2 | 7 | 2 | 7 | 0 | 1 | 0 | 1 | 0 | 1 | 23 | 8 | 7 | 4 | 8 | 10 |
Vac/Vac Membrane | 63 | 29 | 3 | 5 | 29 | 7 | 3 | 4 | 1 | 3 | 4 | 1 | 29 | 23 | 29 | 43 | 31 | 65 | 105 | 22 | 34 | 32 | 48 | 65 |
Unique Cell Count | 351 | 92 | 155 | 161 | 268 | 325 | 426 | 477 | 208 | 391 | 312 | 355 | 178 | 169 | 199 | 173 | 120 | 235 | 480 | 109 | 165 | 135 | 201 | 301 |
Labelled Cell Count | 383 | 132 | 173 | 201 | 315 | 397 | 508 | 585 | 261 | 505 | 411 | 472 | 200 | 187 | 241 | 201 | 139 | 271 | 480 | 109 | 165 | 135 | 201 | 301 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.6 | 3.3 | 3.0 | 3.1 | 3.6 | 3.0 | 3.1 | 3.0 | 2.9 | 2.7 | 2.7 | 2.3 | 4.5 | 4.6 | 3.9 | 5.5 | 5.5 | 6.1 | 4.8 | 3.9 | 4.5 |
Std Deviation (1e-4) | 0.8 | 0.6 | 0.7 | 0.9 | 1.6 | 1.3 | 1.8 | 1.5 | 1.9 | 1.9 | 1.7 | 1.4 | 1.1 | 1.5 | 1.4 | 1.5 | 1.4 | 1.4 | 2.4 | 1.1 | 2.0 |
Intensity Change (Log2) | – | – | – | 0.04 | 0.24 | -0.03 | 0.03 | -0.01 | -0.09 | -0.17 | -0.16 | -0.37 | 0.57 | 0.59 | 0.37 | 0.87 | 0.86 | 1.0 | 0.66 | 0.37 | 0.58 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 6.0 | 6.0 | 6.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.5726 | 1.5643 | 1.6393 | 0.9523 | 0.8789 | 1.3378 | 0.7488 | 1.2859 | 1.2978 | 1.5267 | 0.8404 | 0.761 | -0.8328 | -0.0098 | -0.3329 | 0.8637 | -0.6077 | -0.5416 |
Actin | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0.0507 | 0.0026 | 0.0001 | 0.0326 | 0.0004 | 0.0009 | 0.0245 | 0.0005 | 0.0001 | 0.0002 | 0.0002 | 0.0006 |
Bud | 0.0008 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0011 | 0.0002 | 0 | 0.0008 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0 | 0 | 0.0002 | 0 |
Bud Neck | 0.0012 | 0.0012 | 0.0003 | 0.0008 | 0.0016 | 0.0062 | 0.0015 | 0.0005 | 0.0004 | 0.0004 | 0.0006 | 0.0021 | 0.0012 | 0.0002 | 0.0002 | 0.0002 | 0.0012 | 0.0022 |
Bud Periphery | 0.0008 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0015 | 0.0002 | 0 | 0.0009 | 0 | 0.0002 | 0.0005 | 0 | 0 | 0 | 0.0003 | 0.0001 |
Bud Site | 0.0017 | 0.004 | 0.0003 | 0.0001 | 0.0003 | 0.0003 | 0.0028 | 0.0053 | 0.0002 | 0.0024 | 0.0003 | 0.0002 | 0.0012 | 0.0005 | 0.0001 | 0 | 0.0001 | 0.0001 |
Cell Periphery | 0.0002 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 |
Cytoplasm | 0.1102 | 0.0852 | 0.0345 | 0.0591 | 0.0519 | 0.1143 | 0.0743 | 0.0805 | 0.0188 | 0.0605 | 0.0587 | 0.0942 | 0.064 | 0.0628 | 0.0425 | 0.0282 | 0.0615 | 0.0497 |
Cytoplasmic Foci | 0.004 | 0.0008 | 0.0008 | 0.0005 | 0.0013 | 0.0023 | 0.0264 | 0.0012 | 0.0025 | 0.0169 | 0.0342 | 0.0038 | 0.0075 | 0.0008 | 0.0004 | 0.0008 | 0.0009 | 0.0048 |
Eisosomes | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0003 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0087 | 0.0039 | 0.0015 | 0.003 | 0.0019 | 0.0023 | 0.0057 | 0.0035 | 0.0005 | 0.0013 | 0.004 | 0.0017 | 0.0039 | 0.0011 | 0.0014 | 0.0004 | 0.0012 | 0.0007 |
Endosome | 0.006 | 0.0017 | 0.0013 | 0.0007 | 0.0021 | 0.0017 | 0.0293 | 0.0011 | 0.0008 | 0.0424 | 0.0471 | 0.0058 | 0.0059 | 0.0013 | 0.0005 | 0.0006 | 0.0013 | 0.0024 |
Golgi | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0157 | 0.0004 | 0.0008 | 0.0377 | 0.0027 | 0.0017 | 0.0019 | 0.0001 | 0 | 0 | 0 | 0.0003 |
Lipid Particles | 0.0007 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0174 | 0.0006 | 0.0033 | 0.0198 | 0.0042 | 0.0252 | 0.0038 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0016 |
Mitochondria | 0.0015 | 0.0003 | 0.0004 | 0.0005 | 0.0004 | 0.0005 | 0.0071 | 0.0008 | 0.0004 | 0.0353 | 0.0009 | 0.016 | 0.0015 | 0.0003 | 0.0003 | 0.0002 | 0.0007 | 0.0004 |
None | 0.1215 | 0.0559 | 0.027 | 0.08 | 0.1483 | 0.1268 | 0.0992 | 0.0901 | 0.007 | 0.0653 | 0.0979 | 0.0188 | 0.2754 | 0.1557 | 0.1159 | 0.4356 | 0.2365 | 0.1522 |
Nuclear Periphery | 0.0251 | 0.0085 | 0.0099 | 0.0119 | 0.0135 | 0.0071 | 0.0328 | 0.0137 | 0.0091 | 0.0055 | 0.0145 | 0.0103 | 0.022 | 0.0264 | 0.0114 | 0.0109 | 0.0084 | 0.0075 |
Nucleolus | 0.0115 | 0.0083 | 0.0058 | 0.0027 | 0.0086 | 0.0073 | 0.0128 | 0.003 | 0.0042 | 0.0019 | 0.0035 | 0.0053 | 0.01 | 0.0035 | 0.0053 | 0.0041 | 0.0053 | 0.0084 |
Nucleus | 0.6741 | 0.8067 | 0.908 | 0.8286 | 0.705 | 0.7044 | 0.5704 | 0.7811 | 0.9393 | 0.6639 | 0.6974 | 0.7967 | 0.5559 | 0.7182 | 0.8039 | 0.4577 | 0.6607 | 0.7351 |
Peroxisomes | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0099 | 0.0013 | 0.0065 | 0.0056 | 0.0013 | 0.0021 | 0.0066 | 0.0001 | 0 | 0 | 0 | 0.0013 |
Punctate Nuclear | 0.0216 | 0.02 | 0.0087 | 0.0103 | 0.0626 | 0.0239 | 0.0293 | 0.012 | 0.0051 | 0.0019 | 0.0289 | 0.0127 | 0.0097 | 0.0272 | 0.017 | 0.0606 | 0.0186 | 0.0318 |
Vacuole | 0.0086 | 0.0024 | 0.0008 | 0.0009 | 0.0014 | 0.0018 | 0.0083 | 0.0014 | 0.0007 | 0.0033 | 0.0024 | 0.0016 | 0.0031 | 0.0008 | 0.0006 | 0.0002 | 0.0023 | 0.0006 |
Vacuole Periphery | 0.0012 | 0.0002 | 0.0002 | 0.0004 | 0.0002 | 0.0002 | 0.0033 | 0.0003 | 0.0001 | 0.0014 | 0.0007 | 0.0006 | 0.0008 | 0.0002 | 0.0002 | 0.0001 | 0.0004 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 19.3332 | 5.3394 | 4.5876 | 12.2008 | 14.6729 | 12.2533 | 7.1034 | 5.082 | 9.9604 | 19.4037 |
Translational Efficiency | 1.0536 | 0.7232 | 0.9818 | 0.7357 | 1.0685 | 1.6962 | 0.5471 | 0.6463 | 0.5897 | 0.7276 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1166 | 158 | 1681 | 1025 | 1486 | 1454 | 111 | 1348 | 2652 | 1612 | 1792 | 2373 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 568.48 | 750.33 | 900.13 | 839.80 | 647.99 | 686.00 | 991.30 | 822.87 | 613.03 | 692.31 | 905.78 | 830.18 |
Standard Deviation | 80.68 | 98.36 | 138.17 | 121.33 | 80.83 | 92.54 | 108.79 | 118.46 | 89.89 | 95.08 | 138.29 | 120.00 |
Intensity Change Log 2 | — | 0.400416 | 0.663024 | 0.562936 | — | 0.082237 | 0.613350 | 0.344693 | — | 0.239687 | 0.636777 | 0.450801 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000074 | 0.000881 | 0.000435 | 0.001438 | 0.000076 | 0.000657 | 0.000843 | 0.000502 | 0.000075 | 0.000679 | 0.000461 | 0.000907 |
Bud Neck | 0.008001 | 0.028733 | 0.011395 | 0.019561 | 0.010137 | 0.034833 | 0.003008 | 0.011482 | 0.009198 | 0.034235 | 0.010876 | 0.014972 |
Bud Site | 0.002372 | 0.010312 | 0.007331 | 0.049010 | 0.001556 | 0.015354 | 0.001127 | 0.010481 | 0.001915 | 0.014860 | 0.006947 | 0.027123 |
Cell Periphery | 0.000217 | 0.000356 | 0.000150 | 0.000679 | 0.000243 | 0.000289 | 0.000122 | 0.000208 | 0.000232 | 0.000296 | 0.000148 | 0.000412 |
Cytoplasm | 0.371601* | 0.227997* | 0.047769 | 0.254543* | 0.337628* | 0.318020* | 0.031445 | 0.117880* | 0.352565* | 0.309196* | 0.046757 | 0.176911* |
Cytoplasmic Foci | 0.078063 | 0.281114* | 0.000645 | 0.018565 | 0.107930 | 0.183837* | 0.000858 | 0.003451 | 0.094798 | 0.193372* | 0.000658 | 0.009979 |
Eisosomes | 0.000079 | 0.000270 | 0.000017 | 0.000059 | 0.000075 | 0.000143 | 0.000063 | 0.000032 | 0.000077 | 0.000156 | 0.000020 | 0.000044 |
Endoplasmic Reticulum | 0.000414 | 0.001998 | 0.005275 | 0.003874 | 0.000923 | 0.001189 | 0.014453 | 0.006470 | 0.000699 | 0.001269 | 0.005844 | 0.005349 |
Endosome | 0.002825 | 0.021540 | 0.000508 | 0.019339 | 0.002917 | 0.015976 | 0.000889 | 0.003847 | 0.002877 | 0.016521 | 0.000532 | 0.010539 |
Golgi | 0.000483 | 0.002647 | 0.000068 | 0.019048 | 0.000595 | 0.003425 | 0.000057 | 0.001123 | 0.000546 | 0.003348 | 0.000067 | 0.008866 |
Lipid Particles | 0.004007 | 0.013627 | 0.000266 | 0.001334 | 0.007415 | 0.008884 | 0.000941 | 0.000826 | 0.005917 | 0.009348 | 0.000308 | 0.001045 |
Mitochondria | 0.003502 | 0.005697 | 0.001300 | 0.006701 | 0.005387 | 0.022196 | 0.000670 | 0.005556 | 0.004558 | 0.020579 | 0.001261 | 0.006050 |
Mitotic Spindle | 0.001029 | 0.002538 | 0.004483 | 0.082800 | 0.000995 | 0.004842 | 0.000332 | 0.017129 | 0.001010 | 0.004616 | 0.004226 | 0.045495 |
None | 0.025338 | 0.001872 | 0.005127 | 0.010996 | 0.008882 | 0.006107 | 0.002068 | 0.004436 | 0.016117 | 0.005692 | 0.004937 | 0.007269 |
Nuclear Periphery | 0.000512 | 0.000736 | 0.001421 | 0.003038 | 0.001224 | 0.000808 | 0.001541 | 0.002625 | 0.000911 | 0.000801 | 0.001429 | 0.002804 |
Nuclear Periphery Foci | 0.000250 | 0.000889 | 0.000320 | 0.001934 | 0.000379 | 0.000405 | 0.000484 | 0.000638 | 0.000322 | 0.000453 | 0.000331 | 0.001198 |
Nucleolus | 0.003686 | 0.003289 | 0.000750 | 0.002029 | 0.002759 | 0.003756 | 0.000892 | 0.000731 | 0.003167 | 0.003710 | 0.000759 | 0.001292 |
Nucleus | 0.478674* | 0.293025* | 0.905009* | 0.407469* | 0.492411* | 0.309745* | 0.917829* | 0.777202* | 0.486371* | 0.308106* | 0.905803* | 0.617499* |
Peroxisomes | 0.002476 | 0.040787 | 0.000389 | 0.002014 | 0.005312 | 0.020898 | 0.000199 | 0.000368 | 0.004065 | 0.022847 | 0.000377 | 0.001079 |
Vacuole | 0.016033 | 0.060609 | 0.007197 | 0.078936 | 0.012512 | 0.046675 | 0.022070 | 0.031784 | 0.014060 | 0.048041 | 0.008118 | 0.052151 |
Vacuole Periphery | 0.000365 | 0.001082 | 0.000142 | 0.016629 | 0.000640 | 0.001962 | 0.000108 | 0.003229 | 0.000519 | 0.001876 | 0.000140 | 0.009017 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.93 | -14.69 | -7.81 | -0.11 | -2.96 | -2.67 | -10.05 | -10.35 | 0.22 | 4.45 | -3.05 | -17.27 | -12.13 | -0.28 | -2.01 |
Bud Neck | -3.47 | -3.16 | -3.76 | 2.28 | -1.05 | -8.99 | 4.96 | -1.39 | 8.22 | -6.76 | -10.16 | -2.34 | -3.55 | 8.38 | -0.86 |
Bud Site | -2.42 | -3.67 | -6.82 | -2.49 | -4.75 | -6.97 | -0.89 | -4.93 | 2.97 | -4.70 | -7.13 | -4.22 | -8.36 | 0.45 | -3.95 |
Cell Periphery | -1.78 | 1.91 | -0.39 | 0.78 | -1.04 | -0.43 | 1.04 | 0.85 | 2.14 | -0.64 | -0.87 | 1.45 | 0.39 | 1.34 | -1.09 |
Cytoplasm | 7.19 | 38.14 | 18.30 | 2.84 | -17.70 | 2.06 | 31.78 | 28.68 | 26.24 | -8.83 | 5.16 | 52.96 | 33.05 | 22.93 | -19.30 |
Cytoplasmic Foci | -10.94 | 21.31 | 18.59 | 15.16 | -7.43 | -10.81 | 24.75 | 24.22 | 33.29 | -4.28 | -16.21 | 32.28 | 30.37 | 35.73 | -8.66 |
Eisosomes | -5.04 | 12.91 | 0.57 | 5.13 | -11.16 | -8.98 | -0.49 | 5.78 | 15.31 | 4.21 | -10.73 | 15.40 | 4.53 | 13.78 | -13.38 |
Endoplasmic Reticulum | -5.00 | -20.56 | -19.26 | -11.61 | -1.54 | -1.96 | -10.34 | -22.93 | -21.63 | 5.52 | -4.82 | -21.47 | -29.94 | -25.96 | -1.84 |
Endosome | -4.93 | 7.55 | -3.69 | 3.87 | -6.26 | -9.52 | 6.52 | 1.12 | 9.61 | -2.89 | -10.60 | 11.81 | -2.48 | 9.15 | -7.46 |
Golgi | -2.79 | 3.09 | -3.12 | -1.40 | -3.49 | -5.50 | 3.66 | 0.64 | 5.70 | -2.25 | -6.14 | 4.75 | -2.86 | 1.41 | -3.84 |
Lipid Particles | -4.75 | 9.73 | 6.58 | 6.11 | -12.40 | -1.56 | 10.33 | 10.78 | 13.68 | 1.03 | -4.98 | 14.80 | 12.54 | 14.72 | -10.04 |
Mitochondria | -1.07 | 2.24 | 0.46 | 1.41 | -3.65 | -6.84 | 4.82 | 1.62 | 7.85 | -3.02 | -7.47 | 4.72 | 1.62 | 8.28 | -3.46 |
Mitotic Spindle | -1.46 | -3.26 | -8.94 | -8.35 | -8.03 | -3.75 | 0.22 | -5.70 | -4.05 | -5.79 | -4.00 | -3.64 | -10.53 | -8.76 | -8.62 |
None | 9.59 | 8.56 | 6.98 | -6.61 | -3.73 | 2.05 | 4.81 | 3.75 | 1.85 | -2.60 | 7.27 | 9.04 | 7.88 | -0.21 | -2.88 |
Nuclear Periphery | -1.85 | -16.88 | -18.57 | -15.77 | -12.23 | 2.30 | -3.55 | -12.23 | -15.10 | -6.62 | 0.79 | -9.09 | -21.22 | -20.26 | -15.47 |
Nuclear Periphery Foci | -3.85 | -2.31 | -8.05 | -3.11 | -7.04 | -0.43 | -2.14 | -4.78 | -4.54 | -0.83 | -2.20 | -1.72 | -9.27 | -7.41 | -7.66 |
Nucleolus | 0.76 | 5.17 | 3.71 | 2.20 | -2.91 | -1.36 | 3.57 | 3.37 | 6.24 | -0.54 | -0.71 | 6.02 | 5.00 | 5.97 | -2.44 |
Nucleus | 7.88 | -41.82 | -4.27 | -10.15 | 32.23 | 16.13 | -24.24 | -25.38 | -42.73 | 7.56 | 18.60 | -57.65 | -20.37 | -36.49 | 30.62 |
Peroxisomes | -5.27 | 4.26 | 3.74 | 5.55 | -1.41 | -8.72 | 7.59 | 7.48 | 12.77 | -0.82 | -11.04 | 8.13 | 8.35 | 13.79 | -0.57 |
Vacuole | -5.13 | 3.36 | -19.63 | -6.86 | -20.59 | -12.62 | -3.20 | -13.53 | -4.18 | -1.66 | -13.22 | 0.97 | -23.18 | -12.05 | -23.03 |
Vacuole Periphery | -3.95 | 4.83 | -3.26 | -2.65 | -3.45 | -3.60 | 2.30 | -1.31 | 0.81 | -2.32 | -4.66 | 2.95 | -3.44 | -1.20 | -4.13 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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