Standard name
Human Ortholog
Description Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.05 0.07 0.05 – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Mitochondria 0.06 0 0 0 0 0.06 0.1 0.06 0.12 0.13 0.05 0.09 0 0 0 0.05 0.05 – 0 0 0 0 0 0
Nucleus 0.8 0.88 0.83 0.88 0.86 0.75 0.72 0.69 0.63 0.68 0.63 0.65 0.81 0.83 0.78 0.75 0.79 – 0.72 0.82 0.69 0.63 0.62 0.68
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0.55 0.35 0.47 0.34 0.25 0.51 0.46 0.47 0.51 0.53 0.67 0.65 0.44 0.39 0.37 0.29 0.26 – 0.25 0.11 0.25 0.27 0.27 0.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 2 0
Bud 0 0 0 0 0 0 2 0 0 2 0 2 0 1 1 0 0 – 0 1 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 1 2 0 1 1 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 1 4 11 3 6 13 2 23 0 0 2 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 2 6 4 2 7 9 5 12 9 11 8 4 7 18 15 13 6 – 0 1 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 – 0 0 0 1 0 0
Endosome 0 0 0 1 0 1 0 1 0 0 0 0 0 1 4 0 1 – 0 0 2 0 2 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 – 0 1 0 0 2 5
Mitochondria 6 3 4 3 6 16 39 28 30 47 13 34 0 1 3 8 6 – 0 1 1 0 3 2
Nucleus 77 137 161 68 216 191 286 341 160 244 158 250 104 253 219 131 87 – 66 133 143 67 118 153
Nuclear Periphery 0 0 1 0 0 0 9 6 1 1 0 0 0 0 0 0 1 – 0 0 0 0 0 0
Nucleolus 53 55 92 26 63 129 185 230 130 190 169 247 57 119 103 50 29 – 23 18 52 29 52 45
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 1 0 4 4 1 0 – 0 0 0 0 0 1
SpindlePole 0 0 1 0 0 1 1 5 2 0 1 1 0 3 11 0 1 – 0 0 0 0 0 0
Vac/Vac Membrane 0 1 1 0 1 1 5 5 1 1 0 3 0 1 3 2 0 – 2 3 5 4 4 12
Unique Cell Count 96 155 195 77 252 255 399 493 253 361 252 382 129 305 280 174 110 93 163 208 108 192 227
Labelled Cell Count 138 202 264 100 294 352 543 633 342 509 352 566 168 401 365 206 132 93 163 208 108 192 227


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.0 11.8 10.1 9.8 9.8 8.2 7.4 8.2 7.7 7.3 7.6 7.5 11.5 12.0 11.4 10.0 11.8 – 11.0 10.9 11.8
Std Deviation (1e-4) 2.2 3.0 2.7 3.2 3.0 2.5 2.4 2.6 2.4 2.2 2.2 2.5 3.3 4.0 4.3 2.8 4.2 – 2.6 3.4 2.5
Intensity Change (Log2) – – – -0.04 -0.04 -0.3 -0.44 -0.3 -0.38 -0.47 -0.41 -0.42 0.19 0.26 0.18 -0.01 0.24 – 0.13 0.12 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Bud Site 0 0 0 0 0 0 0 0 3.5 0 0 0 0 0 –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Cytoplasm 0 0 0.9 0 0 0.9 0.7 0.7 0 0 2.0 1.8 2.5 0 –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Mitochondria 0 0 2.2 3.4 2.0 3.9 4.3 1.7 3.1 0 0 0 1.4 0 –
Nucleus 1.2 0.9 -2.0 -2.9 -3.6 -4.5 -3.8 -4.6 -4.3 -0.4 0.1 -1.2 -1.7 -0.7 –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Nucleolus -2.0 -4.9 0.7 -0.2 -0.1 0.9 1.2 4.2 4.0 -0.5 -1.8 -2.3 -3.6 -3.6 –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.0967 7.7478 6.8592 5.8423 5.9072 5.6482 7.502 8.7066 6.9698 6.4711 6.2977 6.8849 6.9009 11.2914 8.689 8.5889 7.8922 8.5773
Actin 0.2321 0 0.0016 0.0003 0 0 0.0045 0 0.0001 0.0209 0.0002 0.0127 0.1863 0 0 0 0.0044 0
Bud 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0037 0 0 0.0005 0.0002 0.0004 0.0003 0.0001 0 0 0.0001 0
Bud Neck 0.001 0 0.0001 0.0002 0.0002 0.001 0.0001 0 0.0001 0.0002 0.0053 0.0007 0.0004 0 0 0 0.0002 0.0002
Bud Periphery 0.0002 0 0.0001 0.0004 0.0001 0.0002 0.0029 0 0.0001 0.0007 0.0005 0.0006 0.0007 0.0001 0 0 0.0002 0
Bud Site 0.0002 0 0.0004 0.0012 0.0001 0.0002 0.0009 0 0.0001 0.0006 0.0004 0.0008 0.0009 0.0001 0 0 0.0004 0
Cell Periphery 0.0001 0 0 0.0006 0 0.0001 0.0002 0 0 0 0.0003 0.0001 0.0003 0 0 0 0.0001 0
Cytoplasm 0.0001 0 0.0002 0.0001 0 0 0.0108 0 0.0002 0.0025 0.0006 0.0087 0.0001 0 0 0 0.0001 0
Cytoplasmic Foci 0.0007 0 0.0025 0.0006 0 0 0.0313 0 0.0102 0.0129 0.002 0.0047 0.0023 0 0 0 0.0857 0
Eisosomes 0.0029 0 0 0.0002 0 0 0.0001 0 0 0.0003 0 0.0001 0.0002 0 0 0 0 0
Endoplasmic Reticulum 0.0008 0 0.0001 0.0001 0 0 0.0006 0 0.0001 0.0004 0.0002 0.0008 0.0002 0 0 0 0 0
Endosome 0.0011 0 0.0072 0.0016 0 0 0.0054 0 0.0004 0.0025 0.0005 0.0061 0.0008 0 0 0 0.0005 0
Golgi 0.0071 0 0.0166 0.0004 0 0 0.0029 0 0.0002 0.0028 0.0002 0.0017 0.0039 0 0 0 0.0018 0
Lipid Particles 0.001 0 0.0001 0.005 0 0 0.0274 0 0.0007 0.0039 0.0044 0.0015 0.0013 0 0 0 0.0054 0
Mitochondria 0.0005 0.0002 0.0054 0.0644 0.0003 0.0001 0.0079 0.0001 0.0003 0.0127 0.0023 0.0013 0.0015 0.0014 0.0004 0.0003 0.0017 0.0002
None 0.0007 0 0 0.0004 0 0 0.0313 0 0.0002 0.0053 0.0005 0.0019 0.0002 0 0 0 0.0001 0
Nuclear Periphery 0.0003 0.0003 0.0196 0.0001 0.0001 0.0001 0.0081 0.0008 0.002 0.0058 0.0048 0.0042 0.0006 0.0002 0.001 0.0002 0.0023 0
Nucleolus 0.0229 0.0302 0.0312 0.0376 0.5258 0.3165 0.0478 0.0257 0.0286 0.0355 0.418 0.1892 0.1785 0.0455 0.0248 0.0717 0.295 0.1864
Nucleus 0.7269 0.9688 0.9128 0.8799 0.4716 0.6811 0.7845 0.973 0.9425 0.8381 0.5511 0.7556 0.6158 0.9521 0.9732 0.9274 0.5319 0.8128
Peroxisomes 0.0007 0 0.0004 0.0025 0 0 0.0191 0 0.0011 0.0316 0.0002 0.0023 0.0037 0 0 0 0.0668 0
Punctate Nuclear 0.0002 0.0002 0.0008 0.0001 0.0014 0.0004 0.0077 0.0002 0.012 0.0227 0.0052 0.0019 0.0008 0 0.0001 0.0001 0.0028 0.0001
Vacuole 0 0 0.0002 0.0033 0.0001 0.0003 0.0015 0 0.0006 0.0001 0.0013 0.0032 0.0008 0.0001 0.0001 0 0.0002 0.0001
Vacuole Periphery 0.0001 0 0.0005 0.0007 0.0001 0 0.0013 0 0.0006 0.0002 0.0016 0.0013 0.0004 0.0002 0.0001 0 0.0003 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.0651 16.9952 18.9168 22.27 25.1234 4.3849 17.8448 20.7425 18.0457 26.2407
Translational Efficiency 1.9957 1.5558 1.0921 1.049 0.9162 4.2929 1.5716 0.9748 1.1879 0.9843

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1614 177 2100 857 1404 702 2328 66 3018 879 4428 923

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 985.81 1207.16 1332.89 1189.13 1046.95 1157.41 1344.03 1455.14 1014.25 1167.43 1338.75 1208.15
Standard Deviation 185.98 256.82 234.36 252.92 209.36 226.77 218.97 257.99 199.55 233.99 226.47 262.40
Intensity Change Log 2 — 0.292235 0.435176 0.270525 — 0.144707 0.360373 0.474965 — 0.218138 0.397134 0.379430

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000428 0.000692 0.000592 0.001631 0.000486 0.000921 0.000616 0.001257 0.000455 0.000875 0.000604 0.001604
Bud Neck 0.005534 0.005179 0.012539 0.027338 0.004340 0.005049 0.011600 0.014745 0.004978 0.005075 0.012046 0.026437
Bud Site 0.000731 0.000369 0.000778 0.006421 0.000423 0.000919 0.000743 0.001418 0.000588 0.000809 0.000760 0.006063
Cell Periphery 0.000058 0.000037 0.000042 0.000268 0.000040 0.000047 0.000027 0.000039 0.000050 0.000045 0.000034 0.000251
Cytoplasm 0.000682 0.000161 0.000024 0.002357 0.000484 0.000457 0.000073 0.000602 0.000590 0.000397 0.000050 0.002231
Cytoplasmic Foci 0.000059 0.000067 0.000029 0.008158 0.000078 0.000370 0.000199 0.011046 0.000068 0.000309 0.000119 0.008365
Eisosomes 0.000075 0.000063 0.000116 0.000080 0.000088 0.000103 0.000108 0.000089 0.000081 0.000095 0.000112 0.000081
Endoplasmic Reticulum 0.001245 0.001182 0.000523 0.001619 0.000967 0.001490 0.000522 0.000931 0.001115 0.001428 0.000522 0.001570
Endosome 0.000124 0.000037 0.000012 0.011980 0.000051 0.000213 0.000169 0.005461 0.000090 0.000178 0.000095 0.011514
Golgi 0.000080 0.000090 0.000061 0.005618 0.000062 0.000180 0.000263 0.006694 0.000072 0.000162 0.000167 0.005695
Lipid Particles 0.000018 0.000027 0.000006 0.001983 0.000019 0.000809 0.000014 0.000046 0.000018 0.000652 0.000011 0.001844
Mitochondria 0.001201 0.001387 0.001281 0.007858 0.000827 0.001954 0.001351 0.003539 0.001027 0.001840 0.001317 0.007549
Mitotic Spindle 0.000658 0.001654 0.000246 0.031191 0.000484 0.009136 0.001494 0.005851 0.000577 0.007629 0.000902 0.029379
None 0.001882 0.001181 0.000442 0.004794 0.000975 0.000938 0.000534 0.001547 0.001460 0.000987 0.000490 0.004562
Nuclear Periphery 0.000211 0.000063 0.000051 0.000919 0.000085 0.000139 0.000112 0.000195 0.000152 0.000124 0.000083 0.000867
Nuclear Periphery Foci 0.000428 0.000100 0.000008 0.000558 0.000142 0.000973 0.000027 0.000041 0.000295 0.000798 0.000018 0.000521
Nucleolus 0.076329 0.121915 0.091280 0.065935 0.115508 0.126930 0.083032 0.106578 0.094556 0.125920 0.086944 0.068842
Nucleus 0.908172* 0.861298* 0.888975* 0.806181* 0.872366* 0.844190* 0.895848* 0.834687* 0.891514* 0.847635* 0.892589* 0.808219*
Peroxisomes 0.000678 0.003984 0.002644 0.004944 0.002031 0.003700 0.001850 0.004188 0.001307 0.003757 0.002226 0.004890
Vacuole 0.000587 0.000204 0.000139 0.007056 0.000237 0.000945 0.000563 0.000698 0.000424 0.000796 0.000362 0.006602
Vacuole Periphery 0.000822 0.000309 0.000211 0.003111 0.000309 0.000536 0.000855 0.000348 0.000583 0.000490 0.000550 0.002913

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.13 -7.85 -10.19 -7.62 -8.78 -4.18 -6.70 -7.33 -3.87 -6.46 -4.96 -10.46 -10.71 -5.87 -9.33
Bud Neck 0.41 -12.48 -11.98 -11.66 -8.23 -1.57 -15.92 -5.95 -5.30 -1.77 -0.53 -19.36 -12.78 -12.35 -8.53
Bud Site 1.57 -0.42 -8.58 -9.73 -8.98 -2.29 -3.17 -4.89 -3.27 -4.14 -1.26 -1.55 -9.34 -8.68 -9.19
Cell Periphery 1.10 0.74 -5.83 -5.75 -5.30 -0.69 1.58 -1.61 -0.76 -3.37 0.22 1.39 -6.18 -6.03 -6.45
Cytoplasm 1.44 1.91 -2.68 -4.31 -4.74 0.50 1.25 0.40 -0.18 -1.70 0.78 2.10 -2.52 -3.37 -4.74
Cytoplasmic Foci -0.09 1.09 -3.82 -3.82 -3.84 -1.80 -0.56 -1.00 -0.95 -0.98 -1.99 -0.43 -3.92 -3.71 -3.89
Eisosomes 1.15 -3.58 -1.48 -1.96 2.93 -2.07 -3.59 -1.37 0.10 0.45 -2.27 -4.90 -0.77 1.63 4.12
Endoplasmic Reticulum 0.15 6.16 -4.52 -1.79 -8.18 -2.26 5.00 -1.97 -0.01 -3.52 -1.61 8.24 -5.55 -2.04 -9.32
Endosome 1.82 2.36 -4.02 -4.08 -4.09 -2.73 -1.25 -1.01 -0.96 -0.98 -1.71 0.07 -4.14 -4.08 -4.14
Golgi -0.37 1.10 -3.35 -3.34 -3.37 -2.82 -1.20 -1.06 -1.03 -1.01 -2.67 -1.08 -3.53 -3.42 -3.42
Lipid Particles -0.36 2.00 -2.10 -2.09 -2.12 -1.38 0.69 -2.25 1.29 -2.68 -1.38 1.47 -2.11 -0.66 -2.12
Mitochondria -0.54 -0.52 -4.28 -4.21 -4.27 -2.00 -6.41 -5.78 -2.08 -4.46 -1.71 -2.28 -4.66 -3.66 -4.37
Mitotic Spindle -0.90 1.29 -7.10 -6.38 -7.22 -3.07 -1.70 -1.39 -0.40 -1.26 -3.13 -1.03 -7.26 -4.64 -7.14
None 1.24 3.10 -3.29 -4.52 -6.02 0.23 3.02 -1.71 -1.79 -2.60 1.69 3.71 -4.27 -5.24 -6.05
Nuclear Periphery 1.61 1.77 -5.34 -8.00 -8.21 -1.14 -0.85 -3.06 -2.12 -2.63 0.34 1.17 -6.70 -7.24 -7.94
Nuclear Periphery Foci 1.31 1.70 -0.17 -4.40 -6.36 -1.77 1.77 0.92 1.99 -2.11 -1.36 2.18 -0.80 1.12 -6.30
Nucleolus -4.33 -4.61 -1.18 3.55 2.27 -1.87 6.57 -0.08 0.57 -1.78 -6.21 1.37 1.85 6.38 1.03
Nucleus 4.16 5.32 11.42 4.08 8.85 3.83 -4.06 2.05 0.85 2.85 7.15 0.99 10.21 4.36 9.91
Peroxisomes -3.73 -10.07 -5.72 -1.01 -2.69 -4.47 0.72 -4.23 -1.58 -4.44 -7.18 -7.55 -5.27 -1.66 -3.72
Vacuole 2.31 2.61 -4.07 -4.43 -4.46 -2.00 -0.87 -3.28 0.09 -1.17 -1.21 0.65 -4.23 -3.90 -4.31
Vacuole Periphery 2.89 3.48 -2.38 -3.59 -3.79 -1.41 -1.30 -0.63 1.09 1.17 0.55 0.23 -2.93 -3.06 -2.83
Standard name
Human Ortholog
Description Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Rsc58

Rsc58


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rsc58-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available