Standard name | |
---|---|
Human Ortholog | |
Description | Microtubule-associated protein (MAP) of the XMAP215/Dis1 family; regulates microtubule dynamics during spindle orientation and metaphase chromosome alignment; interacts with spindle pole body component Spc72p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 1 | 2 | 1 | 1 | 1 | 0 | 3 | 0 | – | 0 | 1 | 1 | 0 | 2 | 2 | 0 | 0 | 1 | 1 | 0 | 3 | 10 | 12 |
Bud | 1 | 3 | 3 | 3 | 2 | 11 | 17 | 22 | 9 | – | 17 | 17 | 3 | 0 | 0 | 3 | 1 | 1 | 3 | 5 | 1 | 9 | 35 | 34 |
Bud Neck | 1 | 9 | 9 | 1 | 5 | 8 | 18 | 18 | 12 | – | 10 | 15 | 7 | 6 | 3 | 2 | 5 | 2 | 0 | 8 | 3 | 4 | 18 | 21 |
Bud Site | 4 | 9 | 10 | 10 | 5 | 14 | 16 | 6 | 20 | – | 28 | 31 | 13 | 4 | 9 | 3 | 1 | 9 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 7 | 4 | 7 | 7 | 15 | 14 | 14 | 11 | 3 | – | 4 | 5 | 16 | 18 | 35 | 24 | 21 | 27 | 0 | 0 | 0 | 0 | 0 | 3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 5 | 0 | 0 | 0 | 0 | 4 | 0 |
Endosome | 7 | 52 | 6 | 4 | 10 | 9 | 4 | 12 | 2 | – | 0 | 3 | 72 | 93 | 121 | 40 | 45 | 31 | 8 | 54 | 25 | 31 | 96 | 80 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 1 | 3 | 0 | 1 | 6 | 4 |
Mitochondria | 12 | 14 | 4 | 3 | 4 | 16 | 29 | 12 | 41 | – | 28 | 17 | 20 | 4 | 4 | 39 | 11 | 20 | 1 | 6 | 4 | 4 | 13 | 12 |
Nucleus | 2 | 4 | 7 | 6 | 8 | 8 | 14 | 9 | 8 | – | 5 | 18 | 8 | 8 | 6 | 3 | 5 | 1 | 2 | 2 | 2 | 1 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 113 | 244 | 127 | 102 | 103 | 136 | 179 | 160 | 142 | – | 123 | 140 | 337 | 228 | 275 | 206 | 210 | 168 | 7 | 37 | 9 | 5 | 14 | 12 |
Peroxisomes | 13 | 48 | 4 | 9 | 4 | 18 | 3 | 13 | 9 | – | 9 | 10 | 79 | 80 | 154 | 15 | 12 | 20 | 4 | 18 | 7 | 32 | 93 | 80 |
SpindlePole | 96 | 250 | 221 | 180 | 185 | 213 | 277 | 276 | 190 | – | 179 | 245 | 343 | 232 | 284 | 118 | 154 | 136 | 124 | 276 | 197 | 139 | 334 | 315 |
Vac/Vac Membrane | 0 | 4 | 0 | 0 | 3 | 0 | 0 | 3 | 2 | – | 0 | 0 | 7 | 11 | 14 | 17 | 21 | 16 | 0 | 1 | 1 | 2 | 6 | 3 |
Unique Cell Count | 169 | 406 | 248 | 217 | 239 | 302 | 380 | 382 | 301 | 257 | 328 | 581 | 406 | 563 | 307 | 320 | 284 | 168 | 442 | 267 | 255 | 677 | 632 | |
Labelled Cell Count | 257 | 642 | 401 | 326 | 346 | 450 | 572 | 545 | 438 | 404 | 502 | 906 | 684 | 908 | 473 | 491 | 439 | 168 | 442 | 267 | 255 | 677 | 632 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.8 | 9.0 | 7.4 | 6.8 | 8.0 | 6.6 | 6.1 | 6.9 | 5.7 | – | 5.5 | 5.9 | 8.2 | 8.5 | 8.5 | 16.2 | 15.2 | 15.0 | 7.5 | 7.4 | 7.6 |
Std Deviation (1e-4) | 1.7 | 1.8 | 1.1 | 1.4 | 2.0 | 1.6 | 1.4 | 1.3 | 1.1 | – | 1.6 | 1.4 | 1.7 | 2.3 | 2.3 | 5.6 | 4.3 | 4.5 | 1.5 | 1.6 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.12 | 0.11 | -0.17 | -0.27 | -0.1 | -0.38 | – | -0.42 | -0.32 | 0.16 | 0.21 | 0.21 | 1.13 | 1.04 | 1.02 | 0.03 | -0.0 | 0.04 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 1.8 | 2.3 | 2.9 | 1.4 | – | 3.1 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | -1.0 | -0.7 | 0.7 | 0.7 | 0.2 | – | 0.2 | 0.6 | 0 | -1.8 | 0 | 0 | -1.6 | -2.4 |
Bud Site | 0.3 | -1.2 | 0.3 | 0.1 | -1.9 | 1.3 | – | 2.9 | 2.5 | -1.4 | -2.6 | -2.1 | -2.4 | 0 | -0.5 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.3 | 1.8 | 1.1 | 0.6 | 0 | 0 | – | -1.0 | -1.1 | -0.1 | 1.0 | 2.0 | 2.5 | 2.0 | 3.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 1.1 | 0.4 | 0 | 0.5 | 0 | – | 0 | 0 | 4.5 | 7.1 | 6.9 | 4.5 | 4.8 | 3.8 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 2.3 | 3.3 | 1.2 | 5.1 | – | 4.3 | 2.3 | 1.4 | 0 | 0 | 4.9 | 1.3 | 3.0 |
Nucleus | 0 | 0.3 | -0.1 | 0.6 | -0.4 | -0.1 | – | -0.6 | 1.6 | 0 | -0.7 | 0 | 0 | -1.0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -0.9 | -1.8 | -1.4 | -1.0 | -2.3 | -0.9 | – | -0.8 | -2.0 | 1.8 | 1.2 | -0.6 | 3.8 | 3.5 | 1.8 |
Peroxisomes | 1.7 | 0 | 2.6 | 0 | 1.4 | 1.1 | – | 1.3 | 1.1 | 5.3 | 6.7 | 8.5 | 2.1 | 1.5 | 3.0 |
SpindlePole | -1.9 | -3.5 | -5.3 | -4.9 | -5.1 | -7.0 | – | -5.4 | -4.4 | -8.5 | -8.6 | -10.5 | -12.2 | -10.2 | -10.1 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3.8 | 4.1 | 3.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.2098 | 6.5261 | 6.3919 | 6.5112 | 4.9229 | 6.1168 | 6.838 | 6.9533 | 7.0456 | 6.9056 | 6.6293 | 6.5318 | 6.0149 | 5.8792 | 6.0341 | 6.5386 | 6.2193 | 5.9871 |
Actin | 0.0158 | 0.0009 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0009 | 0.0003 | 0.0045 | 0.0048 | 0.0014 | 0.0006 | 0.0071 | 0.0001 | 0.0063 | 0.0027 | 0.0033 | 0.0001 |
Bud | 0.0002 | 0.0005 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0002 | 0.0002 | 0.0015 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 |
Bud Neck | 0.0076 | 0.0013 | 0.0003 | 0.0021 | 0.0002 | 0.0002 | 0.0005 | 0.0009 | 0.0003 | 0.0009 | 0.0002 | 0.0002 | 0.005 | 0.0005 | 0.0006 | 0.0009 | 0.0019 | 0.0017 |
Bud Periphery | 0.0004 | 0.0006 | 0.0002 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0003 | 0.0011 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0 |
Bud Site | 0.0035 | 0.0155 | 0.001 | 0.0001 | 0.0001 | 0.0001 | 0.0023 | 0.0073 | 0.0012 | 0.0013 | 0.0025 | 0.0001 | 0.0014 | 0.0009 | 0.0005 | 0.0012 | 0.0004 | 0.0001 |
Cell Periphery | 0.0006 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 |
Cytoplasm | 0.0023 | 0.0018 | 0.0005 | 0.0001 | 0.0001 | 0 | 0.0016 | 0.0001 | 0.0002 | 0.0032 | 0.0001 | 0.0001 | 0.0017 | 0.0001 | 0.0001 | 0.0019 | 0.0002 | 0.0001 |
Cytoplasmic Foci | 0.0891 | 0.01 | 0.0274 | 0.0252 | 0.0874 | 0.0206 | 0.068 | 0.0359 | 0.0116 | 0.0387 | 0.0565 | 0.0129 | 0.0375 | 0.0049 | 0.0065 | 0.0135 | 0.0376 | 0.0104 |
Eisosomes | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0008 | 0.0006 | 0.0009 | 0 | 0 | 0 | 0.0011 | 0 | 0.0001 | 0.0002 | 0.0006 | 0.0001 | 0.0009 | 0 | 0.0002 | 0.0029 | 0.0005 | 0 |
Endosome | 0.0095 | 0.0053 | 0.0032 | 0.0004 | 0.0028 | 0.0003 | 0.0151 | 0.0014 | 0.0043 | 0.0063 | 0.0025 | 0.0016 | 0.0026 | 0.0001 | 0.0033 | 0.0197 | 0.0018 | 0.0019 |
Golgi | 0.0047 | 0.0006 | 0.0006 | 0.0008 | 0.0048 | 0.0009 | 0.0081 | 0.0012 | 0.0038 | 0.0014 | 0.0033 | 0.0024 | 0.0032 | 0.0003 | 0.0021 | 0.0032 | 0.0026 | 0.0009 |
Lipid Particles | 0.0492 | 0.0055 | 0.0137 | 0.0089 | 0.2385 | 0.0177 | 0.0425 | 0.013 | 0.014 | 0.0346 | 0.092 | 0.0147 | 0.0306 | 0.0023 | 0.0079 | 0.0184 | 0.0557 | 0.01 |
Mitochondria | 0.0008 | 0.0005 | 0.0006 | 0 | 0.0002 | 0 | 0.0022 | 0.0001 | 0.0086 | 0.0133 | 0.0077 | 0.0031 | 0.0024 | 0.0003 | 0.0007 | 0.0021 | 0.0096 | 0.0037 |
None | 0.0061 | 0.0014 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0008 | 0.0001 | 0.0003 | 0.0005 | 0.0003 | 0.0001 | 0.0038 | 0.0001 | 0.0002 | 0.0061 | 0.0004 | 0 |
Nuclear Periphery | 0.0013 | 0.0106 | 0.0048 | 0.0008 | 0.0031 | 0.0005 | 0.0023 | 0.0009 | 0.0005 | 0.006 | 0.0025 | 0.0007 | 0.0022 | 0.001 | 0.0016 | 0.0096 | 0.0134 | 0.0008 |
Nucleolus | 0.0112 | 0.118 | 0.0735 | 0.0276 | 0.015 | 0.014 | 0.0489 | 0.0659 | 0.0966 | 0.0213 | 0.0116 | 0.0251 | 0.0482 | 0.0872 | 0.0857 | 0.0226 | 0.0161 | 0.0389 |
Nucleus | 0.0014 | 0.1626 | 0.0228 | 0.0028 | 0.0008 | 0.0019 | 0.0084 | 0.0245 | 0.014 | 0.0128 | 0.0017 | 0.0033 | 0.0102 | 0.0613 | 0.0242 | 0.0122 | 0.0129 | 0.0091 |
Peroxisomes | 0.0149 | 0.0006 | 0.0012 | 0.0011 | 0.0053 | 0.0187 | 0.0057 | 0.0076 | 0.0033 | 0.0052 | 0.0028 | 0.0013 | 0.0072 | 0.0008 | 0.0016 | 0.0037 | 0.0139 | 0.0013 |
Punctate Nuclear | 0.7802 | 0.6588 | 0.8465 | 0.9299 | 0.6407 | 0.9249 | 0.7903 | 0.8405 | 0.8357 | 0.8445 | 0.8122 | 0.9335 | 0.8352 | 0.8398 | 0.8577 | 0.8754 | 0.8279 | 0.9205 |
Vacuole | 0.0003 | 0.0034 | 0.0017 | 0 | 0.0003 | 0 | 0.0004 | 0.0001 | 0.0004 | 0.0014 | 0.0006 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0021 | 0.0004 | 0.0002 |
Vacuole Periphery | 0.0002 | 0.0013 | 0.0008 | 0 | 0.0002 | 0 | 0.0003 | 0 | 0.0002 | 0.0008 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0011 | 0.0008 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 22.9617 | 35.8749 | 10.7032 | 13.5855 | 36.7443 | 27.2907 | 31.2248 | 22.3366 | 17.2587 | 28.4844 |
Translational Efficiency | 1.6346 | 1.2993 | 1.3948 | 1.1032 | 0.8986 | 1.0965 | 1.0841 | 0.95 | 1.0207 | 1.0676 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2334 | 1394 | 1263 | 1459 | 1268 | 635 | 182 | 1392 | 3602 | 2029 | 1445 | 2851 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 769.39 | 1000.34 | 1252.98 | 1219.23 | 848.65 | 944.59 | 1225.09 | 1089.14 | 797.29 | 982.89 | 1249.47 | 1155.71 |
Standard Deviation | 107.46 | 157.33 | 198.99 | 208.86 | 129.59 | 389.08 | 155.34 | 210.67 | 121.77 | 255.05 | 194.26 | 219.59 |
Intensity Change Log 2 | — | 0.378703 | 0.703576 | 0.664183 | — | 0.154519 | 0.529646 | 0.359948 | — | 0.265471 | 0.614970 | 0.512627 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000369 | 0.000741 | 0.000204 | 0.000751 | 0.000781 | 0.000733 | 0.000417 | 0.000992 | 0.000514 | 0.000739 | 0.000231 | 0.000869 |
Bud Neck | 0.002038 | 0.002565 | 0.002502 | 0.005260 | 0.003257 | 0.003251 | 0.001882 | 0.003342 | 0.002467 | 0.002780 | 0.002424 | 0.004323 |
Bud Site | 0.001943 | 0.002673 | 0.002541 | 0.011240 | 0.003838 | 0.003867 | 0.001690 | 0.004128 | 0.002610 | 0.003047 | 0.002434 | 0.007768 |
Cell Periphery | 0.000124 | 0.000080 | 0.000040 | 0.000070 | 0.000198 | 0.000165 | 0.000029 | 0.000066 | 0.000150 | 0.000106 | 0.000039 | 0.000068 |
Cytoplasm | 0.002016 | 0.001742 | 0.002595 | 0.004933 | 0.006500 | 0.003223 | 0.000455 | 0.001642 | 0.003594 | 0.002205 | 0.002326 | 0.003326 |
Cytoplasmic Foci | 0.020586 | 0.013882 | 0.005381 | 0.014260 | 0.033750 | 0.018045 | 0.002271 | 0.011937 | 0.025220 | 0.015185 | 0.004989 | 0.013126 |
Eisosomes | 0.000183 | 0.000112 | 0.000009 | 0.000019 | 0.000198 | 0.000118 | 0.000017 | 0.000044 | 0.000188 | 0.000114 | 0.000010 | 0.000031 |
Endoplasmic Reticulum | 0.001705 | 0.001664 | 0.000384 | 0.000517 | 0.002754 | 0.002042 | 0.000833 | 0.000490 | 0.002074 | 0.001782 | 0.000441 | 0.000504 |
Endosome | 0.002692 | 0.003261 | 0.000704 | 0.002408 | 0.004850 | 0.003585 | 0.000477 | 0.002127 | 0.003452 | 0.003362 | 0.000675 | 0.002271 |
Golgi | 0.004979 | 0.003222 | 0.000428 | 0.001800 | 0.007536 | 0.006015 | 0.000467 | 0.001645 | 0.005879 | 0.004096 | 0.000433 | 0.001724 |
Lipid Particles | 0.038917 | 0.011017 | 0.001264 | 0.001184 | 0.030314 | 0.014699 | 0.001006 | 0.002455 | 0.035888 | 0.012169 | 0.001231 | 0.001805 |
Mitochondria | 0.001129 | 0.000635 | 0.000196 | 0.001848 | 0.001430 | 0.002077 | 0.000235 | 0.001419 | 0.001235 | 0.001086 | 0.000201 | 0.001639 |
Mitotic Spindle | 0.780547* | 0.818957* | 0.959280* | 0.893975* | 0.666250* | 0.790729* | 0.936720* | 0.862545* | 0.740311* | 0.810123* | 0.956439* | 0.878629* |
None | 0.002019 | 0.000547 | 0.000147 | 0.000434 | 0.002880 | 0.000944 | 0.000118 | 0.001218 | 0.002322 | 0.000671 | 0.000143 | 0.000817 |
Nuclear Periphery | 0.000660 | 0.000607 | 0.000378 | 0.000602 | 0.000856 | 0.000612 | 0.000305 | 0.000523 | 0.000729 | 0.000608 | 0.000368 | 0.000563 |
Nuclear Periphery Foci | 0.054225 | 0.047276 | 0.002707 | 0.003488 | 0.085130 | 0.052790 | 0.003431 | 0.008319 | 0.065104 | 0.049001 | 0.002798 | 0.005847 |
Nucleolus | 0.043730 | 0.049054 | 0.010795 | 0.027141 | 0.079912 | 0.050893 | 0.022872 | 0.061624 | 0.056467 | 0.049630 | 0.012316 | 0.043977 |
Nucleus | 0.010014 | 0.013607 | 0.005516 | 0.014451 | 0.027573 | 0.016800 | 0.019214 | 0.014555 | 0.016195 | 0.014606 | 0.007241 | 0.014502 |
Peroxisomes | 0.031323 | 0.026640 | 0.003248 | 0.008004 | 0.040122 | 0.026234 | 0.005794 | 0.019509 | 0.034420 | 0.026513 | 0.003568 | 0.013621 |
Vacuole | 0.000706 | 0.001465 | 0.001583 | 0.004871 | 0.001585 | 0.001650 | 0.001674 | 0.001182 | 0.001016 | 0.001523 | 0.001594 | 0.003070 |
Vacuole Periphery | 0.000095 | 0.000253 | 0.000099 | 0.002745 | 0.000285 | 0.001529 | 0.000093 | 0.000240 | 0.000162 | 0.000653 | 0.000098 | 0.001522 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.40 | -0.52 | -2.52 | -0.28 | -1.81 | 0.33 | -0.15 | -0.82 | -0.92 | -0.58 | -1.17 | 0.24 | -2.36 | -0.76 | -2.16 |
Bud Neck | -2.61 | -5.01 | -9.18 | -7.99 | -4.67 | 0.00 | -0.56 | -3.23 | -2.61 | -1.41 | -1.87 | -4.45 | -9.75 | -8.24 | -3.79 |
Bud Site | -2.79 | -5.05 | -9.07 | -7.93 | -6.36 | 0.17 | 0.63 | -2.71 | -2.28 | -2.59 | -1.84 | -3.93 | -9.64 | -8.20 | -6.00 |
Cell Periphery | 3.28 | 4.57 | 2.69 | -0.41 | -2.34 | 1.28 | 7.60 | 6.69 | 2.55 | -1.96 | 3.24 | 7.57 | 6.34 | 1.99 | -2.48 |
Cytoplasm | 0.50 | -1.76 | -4.17 | -4.27 | -2.23 | 2.10 | 4.69 | 3.39 | 0.92 | -2.62 | 2.15 | 0.22 | -1.36 | -3.18 | -1.24 |
Cytoplasmic Foci | 3.09 | 8.19 | 1.47 | -1.21 | -5.46 | 4.46 | 11.45 | 6.73 | 1.56 | -6.05 | 5.38 | 12.23 | 5.50 | 0.20 | -6.46 |
Eisosomes | 5.76 | 17.51 | 16.79 | 14.05 | -2.56 | 4.02 | 13.19 | 11.37 | 6.46 | -2.43 | 7.10 | 22.55 | 19.36 | 13.57 | -5.01 |
Endoplasmic Reticulum | -0.32 | 7.54 | 5.95 | 5.54 | -0.09 | 2.55 | 1.53 | 13.46 | 7.90 | 1.73 | 1.48 | 8.67 | 12.21 | 9.13 | 0.62 |
Endosome | -0.72 | 3.68 | 0.56 | 1.12 | -3.14 | 1.75 | 6.26 | 3.96 | 2.22 | -1.64 | 0.24 | 6.85 | 2.89 | 2.00 | -3.15 |
Golgi | 4.55 | 15.91 | 8.10 | 4.62 | -2.66 | 1.83 | 13.31 | 10.58 | 6.27 | -2.76 | 4.67 | 20.66 | 13.26 | 7.93 | -4.01 |
Lipid Particles | 10.21 | 14.96 | 15.10 | 7.38 | 0.27 | 4.15 | 10.53 | 10.05 | 6.38 | -1.69 | 10.95 | 18.11 | 17.93 | 9.41 | -1.14 |
Mitochondria | 1.62 | 3.29 | -1.49 | -2.40 | -3.08 | -0.56 | 2.89 | -0.15 | 0.42 | -2.12 | 0.63 | 4.49 | -1.28 | -1.60 | -3.81 |
Mitotic Spindle | -2.87 | -20.20* | -9.46 | -5.71 | 8.93 | -7.04 | -11.81 | -14.94 | -4.93 | 2.83 | -6.88 | -26.86 | -16.19 | -7.25 | 11.27 |
None | 3.98 | 4.77 | 3.57 | -1.06 | -3.55 | 4.99 | 7.51 | 3.13 | -0.28 | -2.00 | 6.00 | 7.63 | 4.14 | -0.86 | -2.67 |
Nuclear Periphery | 1.12 | 1.92 | -1.93 | -2.68 | -3.20 | 3.40 | 3.52 | 2.74 | -0.45 | -1.57 | 2.86 | 3.50 | 0.11 | -2.29 | -3.04 |
Nuclear Periphery Foci | 1.51 | 18.90 | 19.11* | 13.32 | 0.29 | 3.80 | 16.72 | 16.81 | 9.48 | -1.27 | 3.76 | 25.57 | 24.88 | 15.97 | -1.88 |
Nucleolus | -2.08 | 9.69 | 1.59 | 3.27 | -6.49 | 3.78 | 4.75 | 1.86 | -2.22 | -3.61 | 1.05 | 13.33 | 1.15 | 0.04 | -11.01 |
Nucleus | -3.39 | -0.86 | -5.70 | -3.20 | -4.41 | 3.06 | -0.51 | 2.90 | -0.62 | 1.39 | -0.15 | 0.86 | -3.42 | -2.98 | -3.27 |
Peroxisomes | 0.97 | 16.14 | 12.49 | 9.88 | -3.19 | 3.41 | 10.75 | 6.91 | 2.50 | -5.44 | 2.82 | 20.62 | 12.75 | 8.33 | -8.88 |
Vacuole | -4.76 | -3.87 | -5.01 | -3.67 | -1.42 | -0.42 | -1.42 | -0.96 | -0.47 | 1.33 | -4.15 | -3.74 | -5.33 | -3.56 | 0.32 |
Vacuole Periphery | -3.09 | -4.09 | -2.89 | -2.66 | -2.79 | -0.95 | 1.57 | -0.08 | 0.94 | -1.37 | -1.26 | -0.61 | -2.95 | -1.39 | -2.91 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|