ORF
Human Ortholog
Description Putative integral membrane protein; member of DUP240 gene family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.06 0 0.05 0 0 0 0 0 0 0.05 0 0 0.07 0.07 0.07 0.05 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.12
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.05 0 0.06 0 0 0 0.05 0 0 0.09 0.1 0.14 0.24 0.29 0.25 0 0 0 0 0 0
Cytoplasm 0.77 0.46 0.44 0.42 0.48 0.44 0.49 0.41 0.44 0.53 0.41 0.38 0.32 0.28 0.32 0.32 0.29 0.31 0.14 0.14 0.11 0.07
Endoplasmic Reticulum 0 0.39 0.48 0.47 0.45 0.35 0.28 0.33 0.29 0.27 0.53 0.54 0.56 0 0 0 0 0 0.44 0.56 0.59 0.53
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.05 0.08 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0.19 0.12 0.1 0 0 0 0 0 0
Mitochondria 0 0.08 0.06 0.09 0.11 0.18 0.09 0.14 0.3 0.24 0 0 0 0.08 0 0.06 0.19 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.23 0.06 0 0.05 0 0.09 0.17 0.16 0.07 0.09 0 0 0 0.29 0.31 0.31 0.22 0.53 0.21 0.15 0.15 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 11 9 15 16 15 18 16 12 13 21 25 31 22 10 11 1 0 3 2 4 12
Bud 0 2 3 2 3 9 17 12 11 12 0 4 3 4 1 1 0 0 3 14 19 78
Bud Neck 0 0 1 0 0 0 2 0 0 0 0 2 4 0 0 0 0 0 0 2 3 7
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Cell Periphery 0 8 6 17 8 17 12 21 16 14 38 60 100 71 41 39 0 0 0 1 4 8
Cytoplasm 20 78 89 128 201 232 297 164 162 223 182 231 235 82 44 50 6 19 24 46 45 44
Endoplasmic Reticulum 1 66 97 144 190 181 170 132 108 116 236 328 408 4 1 5 0 0 77 183 243 354
Endosome 0 6 7 6 8 14 15 12 2 1 2 9 11 6 4 4 2 3 14 8 6 12
Golgi 0 3 7 3 0 0 1 2 0 1 1 9 3 57 16 16 0 0 0 3 1 3
Mitochondria 0 13 13 28 45 96 54 55 109 101 0 0 0 23 6 10 4 2 9 10 9 15
Nucleus 1 0 0 0 1 0 5 5 1 1 0 0 0 2 2 0 0 0 0 0 0 0
Nuclear Periphery 1 2 2 3 7 12 13 9 14 12 0 1 4 4 4 3 0 0 1 0 1 2
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 2 0 1 0 0 0 0 2 1 0 0 0 0 0 0 0 0
SpindlePole 0 2 1 0 0 2 1 1 0 0 4 3 7 0 1 0 0 0 1 2 4 12
Vac/Vac Membrane 6 11 5 16 18 49 105 63 27 37 7 17 21 86 43 48 4 33 36 49 60 110
Unique Cell Count 26 170 202 304 423 522 612 401 369 424 444 611 728 298 139 155 21 64 176 327 410 669
Labelled Cell Count 29 202 240 362 497 629 710 493 462 531 492 689 829 362 174 187 21 64 176 327 410 669


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 9.0 9.6 9.1 7.8 7.0 7.7 7.6 7.0 7.3 12.1 12.4 12.2 16.4 16.4 16.2 13.1 13.9 12.9
Std Deviation (1e-4) 1.0 5.1 5.5 4.1 3.3 3.1 2.9 2.8 2.4 2.4 5.7 6.0 5.5 4.3 4.6 4.9 6.2 6.1 6.1
Intensity Change (Log2) 0.08 0.0 -0.21 -0.37 -0.24 -0.25 -0.36 -0.31 0.42 0.46 0.43 0.85 0.86 0.84 0.54 0.62 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.9 -0.7 -1.4 -2.1 -2.2 -1.3 -1.7 -1.9 -0.9 -1.3 -1.2 0.4 0.3 0.2
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.9 0.4 0 -0.9 0 0.3 -0.2 -0.8 1.6 2.1 3.3 5.3 5.9 5.2
Cytoplasm -0.4 -0.8 0.4 -0.3 0.6 -1.1 -0.4 1.5 -1.1 -1.9 -3.4 -4.0 -2.5 -2.5
Endoplasmic Reticulum 1.8 1.8 1.4 -1.0 -2.8 -1.4 -2.2 -2.7 3.2 3.4 4.0 -10.9 -8.1 -7.8
Endosome 0 0 0 0 0 -0.3 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 5.4 3.5 3.3
Mitochondria -0.5 0.6 1.1 3.3 0.5 2.0 5.6 4.5 0 0 0 0 -1.2 -0.4
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1.7 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.9 -0.5 -1.1 1.2 3.5 3.0 0.4 0.9 0 -2.3 -2.3 5.7 5.6 5.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.7453 0.9633 0.9757 1.1029 1.0519 1.1506 0.8917 2.2688 1.9086 1.7349 1.6765 1.9696 -0.754 0.0519 -0.2624 -0.4106 -0.0253 -0.1982
Actin 0.0203 0.0003 0.0175 0.0066 0.024 0.0011 0.0024 0.0001 0.0013 0.0004 0.0001 0.0021 0.0214 0.0003 0.0033 0.0059 0.0001 0.0001
Bud 0.0037 0.0012 0.004 0.0027 0.0005 0.0019 0.0011 0.004 0.0021 0.0011 0.0007 0.0007 0.0002 0.001 0.0015 0.0015 0.0001 0.0002
Bud Neck 0.002 0.0055 0.0062 0.0007 0.0003 0.0037 0.0009 0.0008 0.0006 0.0003 0.0002 0.0012 0.0003 0.0003 0.0015 0.0005 0.0002 0.0005
Bud Periphery 0.0016 0.0029 0.0043 0.0051 0.0011 0.0016 0.0033 0.0039 0.0035 0.0014 0.0026 0.0012 0.0004 0.0011 0.0019 0.0012 0.0001 0.0004
Bud Site 0.005 0.0087 0.0094 0.0015 0.0008 0.0005 0.0024 0.007 0.0043 0.0008 0.0003 0.0004 0.0006 0.0023 0.0014 0.0005 0.0002 0.0002
Cell Periphery 0.0005 0.0004 0.0004 0.0003 0.0001 0.0003 0.0007 0.001 0.0006 0.0003 0.0008 0.0002 0.0003 0.0005 0.0002 0.0002 0 0.0001
Cytoplasm 0.1384 0.1994 0.1728 0.1707 0.1349 0.2715 0.1349 0.2226 0.1559 0.0992 0.3102 0.173 0.1842 0.2919 0.2584 0.1602 0.1565 0.3216
Cytoplasmic Foci 0.0521 0.0424 0.0488 0.0687 0.052 0.0557 0.0567 0.052 0.0555 0.0318 0.0281 0.0811 0.0541 0.0636 0.0709 0.0644 0.0381 0.0641
Eisosomes 0.0006 0.0001 0.0002 0.0001 0.0002 0 0.0004 0.0001 0.0002 0.0001 0.0002 0.0001 0.0003 0.0002 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0136 0.0078 0.0118 0.0121 0.0124 0.0073 0.008 0.0073 0.0091 0.0079 0.0061 0.0094 0.0214 0.011 0.0153 0.0099 0.0123 0.006
Endosome 0.156 0.1482 0.1536 0.2945 0.4134 0.1998 0.2232 0.2533 0.2661 0.4289 0.2506 0.3482 0.2553 0.2382 0.2774 0.2654 0.4491 0.2927
Golgi 0.009 0.003 0.0093 0.0176 0.01 0.0048 0.0064 0.0094 0.0085 0.0123 0.0063 0.0186 0.0095 0.0066 0.0088 0.0064 0.0059 0.0044
Lipid Particles 0.0174 0.003 0.0095 0.0037 0.0095 0.0037 0.0138 0.0033 0.0089 0.0046 0.0113 0.0099 0.0111 0.0031 0.0067 0.0019 0.0014 0.002
Mitochondria 0.0127 0.0033 0.0062 0.0084 0.0228 0.0039 0.0111 0.0076 0.0094 0.0051 0.0071 0.0105 0.008 0.0017 0.0038 0.0031 0.0017 0.0023
None 0.4617 0.4956 0.4523 0.2791 0.2035 0.3249 0.4087 0.2772 0.3696 0.2764 0.1975 0.2264 0.3077 0.2524 0.2608 0.3315 0.2086 0.1999
Nuclear Periphery 0.0276 0.0056 0.0072 0.0104 0.0124 0.0118 0.005 0.0032 0.0054 0.0076 0.0035 0.0038 0.0203 0.0063 0.0075 0.0111 0.0038 0.0095
Nucleolus 0.0029 0.0026 0.0013 0.0009 0.0005 0.0005 0.0011 0.003 0.0007 0.001 0.0007 0.0004 0.0005 0.0006 0.0004 0.0008 0.0001 0.0002
Nucleus 0.0114 0.0048 0.005 0.0034 0.0033 0.004 0.0029 0.0064 0.0024 0.0055 0.0025 0.0017 0.0026 0.0035 0.0031 0.0053 0.0013 0.003
Peroxisomes 0.0062 0.0007 0.0093 0.0016 0.0043 0.0007 0.0021 0.0008 0.0016 0.0007 0.0006 0.0032 0.0031 0.0013 0.0022 0.0007 0.0002 0.0006
Punctate Nuclear 0.0135 0.0014 0.0026 0.0021 0.0032 0.0019 0.0029 0.0016 0.0011 0.0026 0.0007 0.0012 0.0015 0.001 0.0014 0.009 0.0003 0.0011
Vacuole 0.0367 0.0549 0.0592 0.0871 0.0679 0.0792 0.0959 0.1224 0.076 0.0939 0.1383 0.0842 0.0818 0.1014 0.0616 0.1058 0.0897 0.0723
Vacuole Periphery 0.0069 0.0082 0.0092 0.0225 0.0227 0.021 0.0163 0.0131 0.017 0.0182 0.0317 0.0225 0.0151 0.0119 0.0117 0.0147 0.0303 0.0188

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.2162 27.9823 18.6877 14.483 13.19 14.0782 15.2074 17.1862 13.4566 19.9702
Translational Efficiency 1.2084 0.7935 1.1338 1.278 1.5337 1.4351 1.7861 1.1801 1.6374 1.084

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1639 1227 1673 1222 1449 268 510 1289 3088 1495 2183 2511

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 724.36 763.88 958.13 860.84 688.85 716.78 863.50 840.55 707.70 755.44 936.02 850.42
Standard Deviation 90.53 102.30 153.37 164.94 285.76 101.68 144.49 147.78 207.32 103.78 156.55 156.70
Intensity Change Log 2 0.076639 0.403515 0.249038 0.057341 0.326006 0.287144 0.067265 0.366254 0.267738

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000125 0.000545 0.000698 0.001229 0.000262 0.000386 0.000680 0.001073 0.000189 0.000517 0.000694 0.001149
Bud Neck 0.020709 0.017455 0.011820 0.014341 0.020080 0.022944 0.020215 0.014164 0.020414 0.018439 0.013781 0.014250
Bud Site 0.008083 0.010039 0.015259 0.039519 0.005396 0.009435 0.021120 0.027697 0.006822 0.009930 0.016628 0.033450
Cell Periphery 0.000416 0.000130 0.000107 0.000271 0.000177 0.000178 0.000163 0.000165 0.000304 0.000139 0.000120 0.000217
Cytoplasm 0.308952* 0.341536* 0.198553* 0.241826* 0.380868* 0.253302* 0.319942* 0.311014* 0.342698* 0.325719* 0.226912* 0.277343*
Cytoplasmic Foci 0.144437 0.258875* 0.082768 0.101950 0.270433* 0.312138* 0.067760 0.102641 0.203559* 0.268423* 0.079262 0.102305
Eisosomes 0.000146 0.000111 0.000028 0.000048 0.000257 0.000217 0.000060 0.000066 0.000198 0.000130 0.000036 0.000057
Endoplasmic Reticulum 0.008026 0.003997 0.014527 0.005112 0.002843 0.002512 0.006531 0.002294 0.005594 0.003731 0.012659 0.003666
Endosome 0.059310 0.066551 0.102329 0.101746 0.049148 0.063090 0.066698 0.093697 0.054541 0.065931 0.094005 0.097614
Golgi 0.002336 0.008641 0.009523 0.021107 0.004427 0.004121 0.001729 0.008007 0.003317 0.007831 0.007702 0.014382
Lipid Particles 0.016671 0.010561 0.008948 0.006387 0.018015 0.020424 0.008886 0.007020 0.017301 0.012329 0.008934 0.006712
Mitochondria 0.003913 0.007531 0.002587 0.003436 0.004601 0.011173 0.002025 0.004149 0.004236 0.008184 0.002456 0.003802
Mitotic Spindle 0.001809 0.005362 0.034075 0.036008 0.001716 0.004397 0.023988 0.019655 0.001765 0.005189 0.031718 0.027613
None 0.002530 0.001909 0.001127 0.003261 0.001340 0.000771 0.002952 0.001957 0.001972 0.001705 0.001553 0.002592
Nuclear Periphery 0.002018 0.000616 0.007947 0.001709 0.000759 0.001124 0.003728 0.001164 0.001427 0.000707 0.006962 0.001429
Nuclear Periphery Foci 0.000639 0.000881 0.016613 0.006092 0.000637 0.000941 0.007580 0.002667 0.000638 0.000892 0.014502 0.004334
Nucleolus 0.001832 0.001286 0.002360 0.001968 0.001062 0.001966 0.002631 0.002416 0.001471 0.001408 0.002424 0.002198
Nucleus 0.119657 0.061463 0.063055 0.057316 0.091523 0.096686 0.184083* 0.082336 0.106456 0.067777 0.091330 0.070160
Peroxisomes 0.003313 0.007050 0.002510 0.007152 0.005962 0.017864 0.003101 0.006586 0.004556 0.008988 0.002648 0.006861
Vacuole 0.287203* 0.188349 0.405710* 0.329091* 0.137393 0.172951 0.250632* 0.304776* 0.216907* 0.185589 0.369480* 0.316609*
Vacuole Periphery 0.007876 0.007113 0.019455 0.020431 0.003100 0.003380 0.005497 0.006457 0.005635 0.006444 0.016194 0.013258

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.73 -4.55 -4.16 -1.53 -1.39 -1.08 -2.12 -1.65 -1.27 -0.42 -1.62 -4.58 -3.54 -1.49 -1.08
Bud Neck 1.82 4.80 4.18 2.17 -0.15 -0.80 -0.02 3.44 2.62 2.11 1.31 4.19 5.41 3.13 0.97
Bud Site -1.49 -4.71 -8.38 -7.95 -5.84 -2.29 -4.53 -7.94 -5.07 -0.98 -3.36 -7.39 -11.39 -9.58 -5.57
Cell Periphery 8.69 9.62 2.63 -1.08 -1.40 -0.05 1.31 2.29 1.75 0.61 8.43 10.00 3.29 -0.94 -1.53
Cytoplasm -3.20 10.64 3.49 6.12 -5.85 8.62 4.99 5.72 -4.23 -0.72 1.87 14.38 6.13 3.36 -7.56
Cytoplasmic Foci -15.48 13.69 10.69 24.26 -2.02 -2.95 32.27 28.03 17.48 -6.31 -9.74 30.98 26.66 29.88 -4.58
Eisosomes 4.69 19.73 15.70 10.89 -5.18 1.34 12.23 12.29 6.31 -0.10 6.83 19.97 17.49 11.46 -6.96
Endoplasmic Reticulum 4.64 -4.89 4.06 -0.35 8.01 0.53 -2.66 1.82 0.66 3.38 3.03 -7.13 4.76 0.89 10.08
Endosome -1.64 -7.70 -3.34 -1.88 3.16 -2.07 -0.03 -5.22 -1.66 -3.81 -3.35 -8.12 -5.96 -2.42 1.69
Golgi -6.22 -6.27 -6.38 -2.83 -3.10 0.36 5.77 -1.36 -1.27 -4.63 -5.15 -4.33 -6.28 -1.80 -2.92
Lipid Particles 4.62 6.10 8.54 5.41 4.11 -0.53 6.39 8.73 3.56 1.29 4.16 9.05 12.14 6.31 4.16
Mitochondria -3.03 2.27 1.75 4.17 -0.36 -1.57 4.10 1.56 1.92 -1.82 -3.23 3.87 2.26 4.32 -1.20
Mitotic Spindle -2.60 -10.80 -8.78 -7.46 0.76 -1.09 -5.31 -6.91 -3.59 1.06 -2.94 -12.08 -11.15 -8.54 2.28
None 0.71 2.46 -0.26 -0.84 -2.13 2.02 -2.89 -1.65 -3.49 1.37 0.43 1.47 -0.76 -0.92 -2.05
Nuclear Periphery 4.74 -8.61 0.15 -6.26 9.00 -1.07 -6.46 -4.37 -0.89 5.18 3.95 -10.64 -1.41 -6.61 10.33
Nuclear Periphery Foci -2.05 -12.72 -6.07 -5.73 7.85 -1.83 -4.53 -4.05 -3.32 2.97 -2.70 -13.46 -7.28 -6.64 9.50
Nucleolus 1.64 -1.29 0.10 -1.38 1.33 -1.35 -4.02 -2.69 -0.67 0.36 0.27 -3.18 -1.95 -2.17 0.75
Nucleus 11.23 8.41 8.06 -2.18 0.12 -0.66 -9.39 0.68 1.00 9.45 9.51 0.83 6.48 -2.97 4.48
Peroxisomes -4.65 2.05 -3.03 1.11 -4.42 -3.54 4.68 0.99 3.86 -4.18 -4.69 5.12 -1.48 3.55 -6.21
Vacuole 10.84 -11.52 -6.46 -16.40 4.08 -3.02 -11.31 -21.94 -11.82 -3.75 4.37 -20.52 -17.97 -20.48 3.17
Vacuole Periphery 1.08 -7.89 -3.83 -4.26 1.40 -0.34 -0.93 -2.80 -1.79 -1.08 -1.11 -9.56 -4.30 -3.10 3.83
ORF
Human Ortholog
Description Putative integral membrane protein; member of DUP240 gene family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS
Localization
Cell Percentages cytoplasm (17%), vacuole (10%), mixed (41%)
Cell Cycle Regulation No
Subcompartmental Group N/A

YAR028W

YAR028W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yar028w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available