Standard name | |
---|---|
Human Ortholog | |
Description | C-5 sterol desaturase; glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; transcriptionally down-regulated when ergosterol is in excess; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of HRD ubiquitin ligase; mutation is functionally complemented by human SC5D |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 1 | 5 | 3 | 11 | 11 | 22 | 16 | 14 | 19 | 21 | 1 | 6 | 10 | 0 | 1 | 0 | 0 | 1 | 2 | 0 | 3 | 2 |
Bud | 0 | 0 | 1 | 1 | 1 | 2 | 4 | 17 | 5 | 6 | 7 | 13 | 0 | 1 | 0 | 1 | 2 | 1 | 2 | 1 | 0 | 2 | 2 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 7 | 3 | 10 | 10 | 14 | 19 | 34 | 21 | 38 | 30 | 50 | 14 | 34 | 63 | 3 | 14 | 13 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 59 | 88 | 56 | 115 | 140 | 226 | 277 | 290 | 230 | 313 | 242 | 348 | 43 | 71 | 83 | 58 | 91 | 75 | 4 | 4 | 3 | 15 | 16 | 25 |
Endoplasmic Reticulum | 130 | 218 | 159 | 141 | 214 | 189 | 305 | 382 | 154 | 247 | 173 | 241 | 186 | 354 | 391 | 0 | 1 | 2 | 200 | 300 | 219 | 229 | 232 | 239 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 2 | 0 | 1 | 4 | 3 |
Golgi | 0 | 1 | 0 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 2 | 1 | 2 | 1 | 2 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 1 | 1 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 1 | 3 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 |
Nuclear Periphery | 1 | 1 | 3 | 3 | 1 | 4 | 4 | 6 | 5 | 11 | 9 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 4 | 2 | 7 | 5 | 3 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 19 | 2 | 12 | 32 | 21 | 15 | 42 | 44 | 29 | 53 | 18 | 29 | 4 | 6 | 7 | 18 | 15 | 9 | 5 | 11 | 9 | 21 | 39 | 51 |
Unique Cell Count | 191 | 299 | 221 | 281 | 362 | 431 | 622 | 742 | 429 | 622 | 455 | 642 | 230 | 420 | 487 | 78 | 114 | 95 | 221 | 331 | 238 | 286 | 310 | 343 |
Labelled Cell Count | 210 | 318 | 235 | 316 | 390 | 465 | 666 | 807 | 464 | 693 | 500 | 712 | 249 | 474 | 560 | 81 | 127 | 102 | 221 | 331 | 238 | 286 | 310 | 343 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 22.9 | 31.3 | 20.1 | 21.3 | 20.8 | 18.7 | 17.5 | 18.8 | 18.7 | 18.3 | 19.3 | 18.8 | 26.4 | 27.8 | 28.1 | 20.1 | 23.2 | 25.4 | 24.1 | 22.4 | 21.9 |
Std Deviation (1e-4) | 5.1 | 6.5 | 4.1 | 8.4 | 4.7 | 4.1 | 4.0 | 4.1 | 4.1 | 4.3 | 4.5 | 4.3 | 5.9 | 6.7 | 6.4 | 7.1 | 7.7 | 8.0 | 5.1 | 5.1 | 5.1 |
Intensity Change (Log2) | – | – | – | 0.08 | 0.05 | -0.1 | -0.2 | -0.09 | -0.1 | -0.14 | -0.05 | -0.09 | 0.39 | 0.47 | 0.49 | 0.0 | 0.21 | 0.34 | 0.27 | 0.16 | 0.13 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 2.1 | 2.5 | 0 | 2.7 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 1.5 | 0 | 1.4 | 1.4 | 2.2 | 2.3 | 2.8 | 3.0 | 3.4 | 2.6 | 3.5 | 4.9 | 0 | 4.3 | 0 |
Cytoplasm | 3.7 | 3.3 | 6.6 | 5.0 | 3.7 | 6.9 | 6.4 | 6.8 | 7.4 | -1.7 | -2.5 | -2.6 | 7.7 | 9.5 | 8.9 |
Endoplasmic Reticulum | -4.9 | -3.1 | -6.8 | -5.9 | -5.4 | -8.7 | -8.2 | -8.3 | -8.8 | 2.2 | 3.7 | 2.5 | -10.9 | -12.3 | -11.4 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 2.3 | 0.2 | -1.2 | 0.7 | 0.3 | 0.7 | 1.5 | -0.9 | -0.6 | -2.1 | -2.9 | -3.0 | 4.5 | 2.5 | 1.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 18.0603 | 24.631 | 23.5414 | 19.691 | 22.2344 | 23.8981 | 23.7994 | 29.482 | 28.2858 | 25.3586 | 25.012 | 26.8573 | 18.711 | 25.1066 | 23.8073 | 22.7364 | 21.663 | 24.4333 |
Actin | 0.0106 | 0.0001 | 0.0024 | 0.0005 | 0.0286 | 0.0021 | 0.0044 | 0.0001 | 0.0049 | 0.0012 | 0.0006 | 0.0001 | 0.015 | 0.0001 | 0.0037 | 0.0194 | 0.0005 | 0.0035 |
Bud | 0.0005 | 0 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0007 |
Bud Neck | 0.0004 | 0.0004 | 0.0012 | 0.0002 | 0.0012 | 0.002 | 0.0001 | 0.0002 | 0.0001 | 0.0011 | 0.0003 | 0.0009 | 0.0004 | 0.0003 | 0.0016 | 0.0047 | 0.0006 | 0.0017 |
Bud Periphery | 0.0005 | 0 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.001 |
Bud Site | 0.0043 | 0.0002 | 0.0013 | 0.0003 | 0.0011 | 0 | 0.003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0016 | 0 | 0.0002 | 0.0003 | 0.0001 | 0.0024 |
Cell Periphery | 0.0012 | 0.009 | 0.0064 | 0.001 | 0.0017 | 0.0016 | 0.0006 | 0.002 | 0.0023 | 0.0019 | 0.0006 | 0.0009 | 0.0005 | 0.0027 | 0.0013 | 0.0004 | 0.0006 | 0.0004 |
Cytoplasm | 0.0772 | 0.1457 | 0.0694 | 0.0574 | 0.0302 | 0.0684 | 0.0205 | 0.0462 | 0.0114 | 0.0122 | 0.0304 | 0.0267 | 0.0295 | 0.0643 | 0.0282 | 0.0307 | 0.1189 | 0.0169 |
Cytoplasmic Foci | 0.0282 | 0.0012 | 0.0086 | 0.0056 | 0.01 | 0.0011 | 0.0041 | 0.0004 | 0.0024 | 0.0106 | 0.0293 | 0.0001 | 0.0065 | 0.0003 | 0.0036 | 0.0015 | 0.0161 | 0.0008 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0005 | 0 | 0.0008 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.5959 | 0.7639 | 0.7909 | 0.7596 | 0.707 | 0.7339 | 0.8636 | 0.873 | 0.9249 | 0.8461 | 0.7897 | 0.8302 | 0.6912 | 0.8748 | 0.8706 | 0.8763 | 0.6335 | 0.8033 |
Endosome | 0.0385 | 0.003 | 0.0165 | 0.0549 | 0.0345 | 0.0067 | 0.0081 | 0.001 | 0.0036 | 0.0042 | 0.0078 | 0.002 | 0.0146 | 0.0007 | 0.0062 | 0.0071 | 0.0096 | 0.005 |
Golgi | 0.0117 | 0.0001 | 0.0055 | 0.004 | 0.0224 | 0.0035 | 0.0009 | 0.0001 | 0.002 | 0.001 | 0.0013 | 0.0001 | 0.0048 | 0 | 0.0037 | 0.0051 | 0.0011 | 0.0006 |
Lipid Particles | 0.0385 | 0.0051 | 0.005 | 0.0157 | 0.0043 | 0.0034 | 0.0042 | 0.002 | 0.0074 | 0.0185 | 0.0097 | 0.0011 | 0.0084 | 0.0036 | 0.0065 | 0.0017 | 0.0075 | 0.0006 |
Mitochondria | 0.0134 | 0.0001 | 0.0216 | 0.0035 | 0.0262 | 0.0032 | 0.0003 | 0 | 0.0033 | 0.0005 | 0.0004 | 0 | 0.0032 | 0 | 0.0019 | 0.0005 | 0.0006 | 0.0007 |
None | 0.0037 | 0.0001 | 0.0017 | 0.0006 | 0.0011 | 0.0001 | 0.0063 | 0.0001 | 0.0001 | 0.0008 | 0.0005 | 0 | 0.0099 | 0 | 0.0038 | 0.0001 | 0.0062 | 0.001 |
Nuclear Periphery | 0.1058 | 0.031 | 0.0343 | 0.0513 | 0.0579 | 0.1202 | 0.0654 | 0.0434 | 0.0252 | 0.0539 | 0.0724 | 0.1016 | 0.1719 | 0.0384 | 0.0488 | 0.0377 | 0.1268 | 0.121 |
Nucleolus | 0.0007 | 0.0001 | 0.0002 | 0.0003 | 0.0006 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0006 | 0 | 0.0003 | 0 | 0.0002 | 0.0009 |
Nucleus | 0.0207 | 0.0039 | 0.0029 | 0.0022 | 0.0222 | 0.0196 | 0.0038 | 0.0089 | 0.0008 | 0.0025 | 0.0037 | 0.0074 | 0.0149 | 0.0023 | 0.0041 | 0.0015 | 0.0073 | 0.0086 |
Peroxisomes | 0.0119 | 0 | 0.0027 | 0.0003 | 0.0016 | 0.0002 | 0.002 | 0 | 0.0039 | 0.005 | 0.0298 | 0 | 0.0048 | 0 | 0.0003 | 0.0005 | 0.0004 | 0.0004 |
Punctate Nuclear | 0.0052 | 0.0002 | 0.0006 | 0.0005 | 0.0051 | 0.0003 | 0.0011 | 0.0001 | 0.0002 | 0.001 | 0.0011 | 0.0001 | 0.0049 | 0.0001 | 0.0009 | 0.0002 | 0.0019 | 0.0044 |
Vacuole | 0.027 | 0.0351 | 0.0265 | 0.029 | 0.0387 | 0.0313 | 0.0098 | 0.0206 | 0.0065 | 0.0361 | 0.0186 | 0.0267 | 0.0122 | 0.0119 | 0.0119 | 0.0107 | 0.0647 | 0.0238 |
Vacuole Periphery | 0.0037 | 0.0006 | 0.0017 | 0.0128 | 0.0049 | 0.002 | 0.0014 | 0.0014 | 0.0006 | 0.003 | 0.0038 | 0.0018 | 0.0041 | 0.0003 | 0.0015 | 0.0012 | 0.003 | 0.0021 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 328.6095 | 475.2213 | 389.2981 | 397.5177 | 375.894 | 223.9736 | 533.859 | 462.238 | 427.2079 | 529.9926 |
Translational Efficiency | 1.2055 | 1.2101 | 1.3105 | 0.9522 | 1.1368 | 1.4883 | 1.1929 | 1.2183 | 1.2613 | 1.1365 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
409 | 1513 | 112 | 1394 | 2071 | 1757 | 1785 | 1877 | 2480 | 3270 | 1897 | 3271 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 2855.01 | 1839.67 | 3188.74 | 2619.61 | 2123.95 | 1700.28 | 2029.95 | 3048.48 | 2244.52 | 1764.77 | 2098.37 | 2865.71 |
Standard Deviation | 802.18 | 483.48 | 1069.51 | 545.72 | 585.84 | 342.30 | 438.25 | 661.25 | 682.89 | 419.46 | 568.20 | 650.23 |
Intensity Change Log 2 | — | -0.634049 | 0.159491 | -0.124144 | — | -0.320977 | -0.065306 | 0.521340 | — | -0.492115 | 0.067843 | 0.187018 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000201 | 0.000261 | 0.000364 | 0.000518 | 0.000160 | 0.000531 | 0.000624 | 0.000639 | 0.000167 | 0.000406 | 0.000609 | 0.000587 |
Bud Neck | 0.001224 | 0.001081 | 0.014320 | 0.005698 | 0.001047 | 0.000355 | 0.003588 | 0.004675 | 0.001076 | 0.000691 | 0.004221 | 0.005111 |
Bud Site | 0.001101 | 0.001986 | 0.001137 | 0.008309 | 0.002674 | 0.002216 | 0.005946 | 0.006909 | 0.002414 | 0.002110 | 0.005662 | 0.007506 |
Cell Periphery | 0.015182 | 0.002756 | 0.010273 | 0.021827 | 0.010098 | 0.003592 | 0.010346 | 0.014298 | 0.010936 | 0.003205 | 0.010341 | 0.017507 |
Cytoplasm | 0.002839 | 0.023300 | 0.005363 | 0.012079 | 0.056948 | 0.014787 | 0.101405* | 0.017931 | 0.048025 | 0.018726 | 0.095735 | 0.015437 |
Cytoplasmic Foci | 0.000370 | 0.000857 | 0.003296 | 0.001467 | 0.002256 | 0.001861 | 0.001697 | 0.002561 | 0.001945 | 0.001396 | 0.001792 | 0.002095 |
Eisosomes | 0.000733 | 0.000020 | 0.000073 | 0.000137 | 0.000013 | 0.000018 | 0.000045 | 0.000091 | 0.000131 | 0.000019 | 0.000047 | 0.000110 |
Endoplasmic Reticulum | 0.896858* | 0.889662* | 0.544055* | 0.749855* | 0.718229* | 0.916696* | 0.677446* | 0.751060* | 0.747688* | 0.904187* | 0.669570* | 0.750546* |
Endosome | 0.008460 | 0.011610 | 0.019429 | 0.023468 | 0.012812 | 0.007957 | 0.014729 | 0.022237 | 0.012095 | 0.009647 | 0.015007 | 0.022762 |
Golgi | 0.002514 | 0.001428 | 0.006483 | 0.004388 | 0.005289 | 0.001716 | 0.006397 | 0.004627 | 0.004831 | 0.001583 | 0.006402 | 0.004525 |
Lipid Particles | 0.004514 | 0.003230 | 0.025119 | 0.059858 | 0.004413 | 0.001802 | 0.028262 | 0.049819 | 0.004430 | 0.002463 | 0.028077 | 0.054097 |
Mitochondria | 0.000255 | 0.000330 | 0.002281 | 0.000841 | 0.000517 | 0.000359 | 0.000769 | 0.001049 | 0.000474 | 0.000345 | 0.000858 | 0.000961 |
Mitotic Spindle | 0.000445 | 0.000078 | 0.000568 | 0.003211 | 0.000101 | 0.000112 | 0.000887 | 0.006342 | 0.000157 | 0.000096 | 0.000868 | 0.005008 |
None | 0.000757 | 0.001511 | 0.000128 | 0.001557 | 0.002373 | 0.000804 | 0.003412 | 0.001959 | 0.002106 | 0.001131 | 0.003218 | 0.001788 |
Nuclear Periphery | 0.004214 | 0.004145 | 0.146585* | 0.005413 | 0.005328 | 0.001364 | 0.007406 | 0.007024 | 0.005144 | 0.002651 | 0.015623 | 0.006337 |
Nuclear Periphery Foci | 0.010305 | 0.016741 | 0.109266 | 0.040671 | 0.039781 | 0.005427 | 0.075626 | 0.050175 | 0.034920 | 0.010662 | 0.077612 | 0.046124 |
Nucleolus | 0.000039 | 0.000056 | 0.000044 | 0.000060 | 0.000124 | 0.000067 | 0.000055 | 0.000134 | 0.000110 | 0.000062 | 0.000054 | 0.000103 |
Nucleus | 0.000522 | 0.001316 | 0.001770 | 0.001142 | 0.001838 | 0.000515 | 0.003575 | 0.001556 | 0.001621 | 0.000886 | 0.003469 | 0.001380 |
Peroxisomes | 0.000007 | 0.000024 | 0.000026 | 0.000093 | 0.000069 | 0.000076 | 0.000074 | 0.000292 | 0.000059 | 0.000052 | 0.000071 | 0.000207 |
Vacuole | 0.045947 | 0.037969 | 0.103543 | 0.057053 | 0.123401 | 0.037837 | 0.055118 | 0.053971 | 0.110627 | 0.037898 | 0.057977 | 0.055284 |
Vacuole Periphery | 0.003513 | 0.001640 | 0.005875 | 0.002356 | 0.012531 | 0.001909 | 0.002591 | 0.002651 | 0.011043 | 0.001785 | 0.002785 | 0.002525 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.88 | -0.88 | -2.74 | -1.52 | -0.17 | -1.17 | -4.88 | -2.80 | -1.18 | -0.78 | -1.54 | -4.91 | -3.69 | -1.63 | -0.43 |
Bud Neck | 0.39 | -5.34 | -7.11 | -7.54 | 3.18 | 6.49 | -7.68 | -8.75 | -10.05 | -3.00 | 2.71 | -8.96 | -11.66 | -12.41 | -2.62 |
Bud Site | -1.62 | -0.21 | -6.04 | -5.41 | -6.16 | 0.81 | -4.95 | -5.60 | -6.00 | -1.47 | 0.62 | -5.30 | -7.52 | -8.11 | -2.28 |
Cell Periphery | 4.30 | 1.10 | -1.95 | -11.60 | -3.77 | 6.42 | -0.51 | -3.20 | -10.05 | -3.01 | 8.25 | 0.21 | -5.22 | -15.39 | -6.04 |
Cytoplasm | -8.91 | -1.31 | -7.00 | 3.51 | -2.76 | 12.43 | -8.90 | 9.76 | -3.03 | 17.49 | 9.92 | -10.33 | 10.07 | 0.24 | 18.45 |
Cytoplasmic Foci | -2.06 | -1.02 | -3.80 | -2.83 | 0.50 | 0.86 | 0.94 | -1.64 | -2.12 | -2.29 | 1.76 | -0.06 | -1.65 | -3.19 | -1.37 |
Eisosomes | 1.32 | 1.21 | 1.10 | -4.85 | -2.05 | -1.50 | -4.31 | -7.54 | -6.87 | -3.56 | 1.26 | 0.93 | 0.21 | -7.68 | -4.63 |
Endoplasmic Reticulum | 0.66 | 10.49 | 11.60 | 14.87 | -5.83 | -23.07* | 5.32 | -0.70 | 20.82 | -5.78 | -20.92 | 9.17 | 1.96 | 25.29 | -7.80 |
Endosome | -2.73 | -4.40 | -10.34 | -9.22 | -1.62 | 6.32 | -3.05 | -9.10 | -13.39 | -7.12 | 3.84 | -4.67 | -12.72 | -15.95 | -8.87 |
Golgi | 1.25 | -1.93 | -2.01 | -5.13 | 1.07 | 5.34 | -1.66 | 0.81 | -4.71 | 2.63 | 5.86 | -2.48 | 0.43 | -6.84 | 3.35 |
Lipid Particles | 1.12 | -2.60 | -13.89 | -14.47 | -3.66 | 4.30 | -11.14 | -15.10 | -16.06 | -5.91 | 3.42 | -11.47 | -20.61 | -21.57 | -8.16 |
Mitochondria | -0.37 | -1.56 | -3.12 | -3.99 | 1.14 | 1.97 | -3.07 | -2.27 | -3.03 | -0.99 | 1.90 | -3.54 | -3.30 | -4.44 | -0.37 |
Mitotic Spindle | 0.81 | -0.17 | -3.00 | -3.53 | -3.04 | -0.15 | -1.77 | -5.27 | -5.26 | -4.34 | 0.61 | -1.67 | -6.26 | -6.33 | -4.82 |
None | -0.85 | 1.03 | -1.34 | -0.15 | -3.83 | 2.78 | -1.31 | 0.62 | -2.19 | 1.90 | 1.82 | -1.54 | 0.58 | -1.53 | 2.17 |
Nuclear Periphery | 0.19 | -8.24 | -1.69 | -2.01 | 8.16 | 7.36 | -3.10 | -2.55 | -10.06 | 0.28 | 4.89 | -7.48 | -2.41 | -8.29 | 6.51 |
Nuclear Periphery Foci | -2.23 | -7.94 | -8.28 | -7.54 | 5.26 | 13.37* | -8.70 | -3.56 | -14.65 | 4.77 | 10.78 | -11.01 | -4.60 | -15.95 | 7.25 |
Nucleolus | -0.58 | -0.33 | -1.05 | -0.10 | -1.08 | 1.19 | 3.16 | -0.42 | -1.04 | -1.31 | 1.26 | 2.75 | -0.20 | -1.01 | -1.36 |
Nucleus | -1.74 | -1.37 | -2.16 | -0.43 | 0.53 | 4.72 | -4.70 | -1.03 | -5.19 | 3.56 | 2.37 | -5.27 | -1.16 | -3.59 | 4.37 |
Peroxisomes | -3.74 | -0.92 | -3.09 | -2.44 | -2.03 | -0.22 | -0.07 | -2.03 | -1.91 | -1.89 | 0.22 | -0.24 | -2.27 | -2.38 | -1.83 |
Vacuole | 1.09 | -4.03 | -2.08 | -5.18 | 3.23 | 16.62 | 11.87 | 11.15 | -5.79 | -0.07 | 16.72 | 9.77 | 10.64 | -7.77 | 0.70 |
Vacuole Periphery | 1.47 | -0.93 | 0.86 | -1.78 | 1.73 | 11.14 | 10.37 | 10.05 | -1.93 | -0.14 | 11.36 | 9.77 | 10.12 | -2.66 | 0.70 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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