Standard name
Human Ortholog
Description 3'-5' RNA exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.07 0 0.05 0.1 0 0.06 0.07 0 0 0 0.07 0.09 0.07 0 0 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.08 0 0 0.05 0.05 0.07 0 0.2 0.11 0.2 0.22 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.9 0.8 0.8 0.76 0.77 0.74 0.8 0.8 0.7 0.68 0.7 0.63 0.81 0.77 0.79 0.79 0.81 0.74 0.75 0.7 0.54 0.56 0.46 0.33
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0.23 0.08 0.1 0.12 0.2 0.28 0.35 0.25 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0.12 0.11 0.13 0 0.06 0.07 0.05 0.05 0.06 0 0.06 0.1 0.11 0.09 0.17 0.12 0.17 0.2 0.23 0.32 0.37 0.45 0.56
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0
Bud 0 0 0 0 0 1 0 0 2 2 0 0 0 0 0 0 0 0 1 3 0 4 1 3
Bud Neck 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 1 1 1 2 1 1 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1 3 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 8 3 2 1 10 4 11 10 5 1 3 15 30 21 8 7 13 0 1 3 2 2 5
Endoplasmic Reticulum 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 6 4 6 0 0 0 1 0 1
Endosome 0 1 0 0 0 4 0 1 1 1 0 0 3 5 4 1 1 3 2 4 0 2 3 3
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 1 0 2 6 6 3 2 1 1 1 1 3
Mitochondria 3 20 1 2 1 5 8 2 30 13 9 23 1 2 9 4 1 0 1 6 2 2 6 5
Nucleus 283 209 36 35 17 73 96 150 103 84 32 67 186 270 241 238 130 160 261 204 47 163 141 99
Nuclear Periphery 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0
Nucleolus 5 4 2 2 5 8 12 22 29 34 16 26 1 6 5 3 1 3 1 0 0 1 4 7
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 3 4 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2 0 0 0 1 0
Vac/Vac Membrane 29 31 5 6 0 6 8 9 8 7 1 6 23 40 28 51 20 37 69 67 27 108 136 169
Unique Cell Count 314 261 45 46 22 99 120 187 148 123 46 106 229 352 305 302 161 216 347 294 87 293 304 304
Labelled Cell Count 324 273 47 47 24 107 130 197 184 148 60 126 232 359 315 317 170 225 347 294 87 293 304 304


Mitochondria, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.9 10.2 13.9 12.1 8.4 11.2 10.3 11.5 10.2 10.1 9.7 9.9 14.9 14.3 13.1 13.0 17.3 17.1 15.2 16.0 16.8
Std Deviation (1e-4) 3.4 2.7 3.7 3.6 2.5 3.0 2.9 2.9 2.8 2.8 2.6 3.0 3.8 4.2 3.9 3.5 4.9 5.3 3.6 3.5 4.1
Intensity Change (Log2) -0.19 -0.72 -0.31 -0.44 -0.28 -0.45 -0.46 -0.52 -0.49 0.1 0.05 -0.08 -0.1 0.32 0.3 0.13 0.21 0.28


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 2.9 0 0 3.0 0 0 0 0 0 0
Nucleus -0.5 -0.3 -0.8 0 0 -1.4 -1.5 -1.1 -2.0 0.2 -0.5 -0.2 -0.2 0.1 -0.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 2.4 3.2 3.6 2.9 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.3 0 0 0 0 0 0 0 0 0 0.1 0 1.0 0.2 1.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.8385 9.1405 8.1824 8.7135 8.9609 8.5398 14.7519 15.5878 15.0425 14.1376 14.214 14.9282 10.7236 12.5163 10.6813 9.7334 12.2613 11.7088
Actin 0.0476 0.0019 0.0308 0.0464 0.0543 0.0139 0.0006 0.0055 0.0019 0.0025 0.0032 0.0024 0.0161 0.001 0.0121 0.0025 0.0006 0.0034
Bud 0.0014 0.0004 0.0009 0.0016 0.0013 0.0008 0.0002 0.0006 0.0004 0.0008 0.0008 0.0008 0.0004 0.0006 0.0004 0.0006 0.0006 0.0004
Bud Neck 0.0016 0.0003 0.0027 0.0005 0.0019 0.0036 0.0008 0.0005 0.0005 0.0002 0.0019 0.0061 0.0004 0.0004 0.0005 0.0005 0.0038 0.0025
Bud Periphery 0.0022 0.0007 0.0012 0.0036 0.0043 0.0031 0.0006 0.0012 0.0008 0.002 0.0037 0.0034 0.0005 0.0014 0.0009 0.0013 0.0019 0.0011
Bud Site 0.021 0.0003 0.0095 0.0019 0.0038 0.0004 0.0009 0.0011 0.0006 0.0005 0.0009 0.0005 0.0008 0.0004 0.0009 0.0006 0.0004 0.0002
Cell Periphery 0.001 0.0004 0.0004 0.0013 0.0029 0.0009 0.0007 0.0006 0.0004 0.0003 0.0016 0.0009 0.0002 0.0005 0.0003 0.0003 0.001 0.0003
Cytoplasm 0.0064 0.001 0.004 0.0028 0.0012 0.0012 0.0016 0.0015 0.0025 0.0021 0.0011 0.0016 0.0061 0.0013 0.0061 0.0033 0.001 0.0008
Cytoplasmic Foci 0.0143 0 0.0023 0.0102 0.0062 0.0005 0.001 0.0002 0.0004 0.0189 0.0001 0.0005 0.0138 0.0001 0.0046 0.0017 0 0.0036
Eisosomes 0.0003 0 0.0001 0.0002 0.0004 0.0001 0 0.0001 0 0.0001 0.0001 0 0.0001 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0082 0.0004 0.0005 0.002 0.0034 0.0013 0.0008 0.0012 0.0012 0.0016 0.0031 0.0019 0.0024 0.0005 0.0035 0.0078 0.0008 0.0004
Endosome 0.0152 0.0003 0.0016 0.0091 0.0028 0.003 0.0006 0.002 0.0037 0.0115 0.0032 0.0041 0.0259 0.0007 0.0062 0.0183 0.0007 0.0006
Golgi 0.0052 0.0001 0.0035 0.0043 0.0036 0.0017 0.0002 0.0004 0.0003 0.0016 0.0004 0.0005 0.0102 0.0001 0.0035 0.003 0 0.0006
Lipid Particles 0.018 0 0.0029 0.004 0.0178 0.0012 0.0026 0.0002 0.0009 0.0125 0.0007 0.0013 0.0142 0.0001 0.0037 0.0049 0.0001 0.0005
Mitochondria 0.0302 0.0239 0.0618 0.0782 0.1508 0.0123 0.0245 0.0461 0.033 0.1347 0.0712 0.0153 0.0206 0.0268 0.0338 0.0308 0.0223 0.0108
None 0.0063 0.0001 0.0002 0.0028 0.0004 0.0003 0.0002 0.0002 0.0008 0.0047 0.0005 0.0003 0.0027 0.0001 0.0029 0.0006 0.0002 0.0001
Nuclear Periphery 0.0287 0.0207 0.0242 0.0227 0.0416 0.0228 0.0274 0.0355 0.0261 0.0133 0.0763 0.0332 0.0364 0.0302 0.0124 0.0571 0.0567 0.0204
Nucleolus 0.0208 0.0152 0.0078 0.0095 0.0437 0.0228 0.015 0.0187 0.0098 0.0077 0.0392 0.0274 0.0099 0.0114 0.0118 0.014 0.027 0.0178
Nucleus 0.7241 0.928 0.836 0.7671 0.5982 0.8953 0.9122 0.8691 0.9053 0.7665 0.7568 0.8805 0.8031 0.9114 0.8857 0.8361 0.85 0.9254
Peroxisomes 0.0031 0.0001 0.0022 0.0062 0.0245 0.0004 0.0002 0.0003 0.0003 0.0015 0.0004 0.0002 0.0116 0.0002 0.0027 0.0003 0.0001 0.0035
Punctate Nuclear 0.0315 0.0002 0.0003 0.0187 0.0003 0.0009 0.002 0.0022 0.0019 0.002 0.0008 0.0026 0.019 0.0003 0.0014 0.0031 0.0004 0.0004
Vacuole 0.0041 0.0017 0.0019 0.0014 0.0119 0.0071 0.0027 0.0042 0.0048 0.0039 0.0104 0.0077 0.0023 0.0041 0.0028 0.0052 0.0099 0.0034
Vacuole Periphery 0.0088 0.0043 0.0053 0.0054 0.0245 0.0064 0.0052 0.0087 0.0044 0.0112 0.0236 0.0086 0.0032 0.0083 0.0039 0.0081 0.0224 0.0041

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 36.7801 47.4922 44.2775 43.9359 25.6097 25.868 48.3271 41.551 42.511 37.515
Translational Efficiency 1.073 0.9999 0.9475 0.7639 1.2721 1.459 0.9409 1.1688 0.9746 1.0538

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
175 365 204 2401 1582 2365 186 1420 1757 2730 390 3821

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1245.74 1266.76 1768.92 1938.40 1213.45 1375.89 2066.86 2039.20 1216.67 1361.30 1911.01 1975.86
Standard Deviation 254.23 252.87 365.85 396.53 237.29 228.98 386.15 412.94 239.22 235.27 404.07 405.64
Intensity Change Log 2 0.024140 0.505866 0.637863 0.181250 0.768326 0.748889 0.103802 0.641337 0.693715

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.019632 0.010612 0.010304 0.006485 0.007312 0.005586 0.012688 0.015979 0.008539 0.006258 0.011441 0.010013
Bud Neck 0.100607 0.082100 0.078516 0.205390* 0.052376 0.075989 0.080664 0.196837* 0.057180 0.076806 0.079540 0.202211*
Bud Site 0.045304 0.022795 0.015053 0.021410 0.011619 0.012500 0.015479 0.033831 0.014974 0.013876 0.015256 0.026026
Cell Periphery 0.000688 0.000740 0.000313 0.000745 0.000528 0.001123 0.000326 0.001190 0.000544 0.001072 0.000319 0.000910
Cytoplasm 0.006870 0.000836 0.000403 0.003381 0.000519 0.000441 0.000190 0.002033 0.001152 0.000494 0.000301 0.002880
Cytoplasmic Foci 0.007486 0.001932 0.001541 0.001483 0.000992 0.000538 0.001699 0.002368 0.001639 0.000724 0.001616 0.001812
Eisosomes 0.000349 0.000388 0.000529 0.000129 0.000348 0.000276 0.000893 0.000607 0.000348 0.000291 0.000703 0.000306
Endoplasmic Reticulum 0.054770 0.032640 0.007914 0.013315 0.018540 0.057526 0.008152 0.013879 0.022148 0.054199 0.008027 0.013525
Endosome 0.003588 0.002057 0.000276 0.002595 0.001258 0.001839 0.000247 0.000940 0.001490 0.001868 0.000262 0.001980
Golgi 0.002362 0.001627 0.000229 0.001431 0.000559 0.000493 0.000178 0.001658 0.000739 0.000645 0.000205 0.001516
Lipid Particles 0.009360 0.001544 0.000457 0.000673 0.001032 0.000887 0.000582 0.001465 0.001861 0.000975 0.000516 0.000967
Mitochondria 0.234603* 0.057658 0.047264 0.078878 0.058008 0.048711 0.058835 0.119850* 0.075597 0.049907 0.052783 0.094104
Mitotic Spindle 0.057882 0.005081 0.002626 0.022227 0.006277 0.002337 0.007380 0.006017 0.011417 0.002704 0.004893 0.016202
None 0.002201 0.002984 0.001851 0.002467 0.003615 0.001727 0.001093 0.003132 0.003474 0.001895 0.001490 0.002714
Nuclear Periphery 0.005241 0.001731 0.001020 0.013906 0.002561 0.006453 0.000878 0.005756 0.002828 0.005822 0.000952 0.010877
Nuclear Periphery Foci 0.004000 0.001999 0.000346 0.004083 0.001411 0.001045 0.000280 0.001923 0.001669 0.001173 0.000314 0.003281
Nucleolus 0.033309 0.039866 0.043283 0.022180 0.074572 0.027372 0.055315 0.034650 0.070462 0.029043 0.049021 0.026814
Nucleus 0.345714* 0.701236* 0.758150* 0.571663* 0.729728* 0.722919* 0.718103* 0.524100* 0.691479* 0.720020* 0.739051* 0.553987*
Peroxisomes 0.022170 0.009241 0.020605 0.002094 0.013461 0.004419 0.027800 0.007132 0.014328 0.005063 0.024037 0.003967
Vacuole 0.031900 0.018616 0.006703 0.017156 0.009811 0.021609 0.007708 0.020329 0.012011 0.021208 0.007182 0.018335
Vacuole Periphery 0.011964 0.004319 0.002618 0.008309 0.005474 0.006210 0.001509 0.006325 0.006120 0.005957 0.002089 0.007572

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 4.01 3.98 6.47 3.84 3.52 4.60 -4.57 -10.34 -12.71 -1.78 5.40 -3.64 -3.10 -9.09 2.01
Bud Neck 1.58 1.31 -11.26 -17.65 -13.90 -7.85 -4.42 -28.94 -24.13 -14.30 -6.71 -4.62 -40.95 -35.52 -20.01
Bud Site 3.90 5.27 4.21 -0.04 -3.35 -1.04 -2.42 -14.29 -13.89 -7.59 0.92 -0.86 -10.71 -13.15 -7.18
Cell Periphery -0.38 5.35 -0.14 0.21 -3.74 -5.61 3.18 -3.10 -0.08 -3.95 -5.63 4.83 -3.29 1.58 -5.41
Cytoplasm 2.16 2.30 1.32 -6.84 -11.53 0.62 3.49 -4.67 -4.83 -5.54 2.18 2.78 -4.59 -11.53 -12.72
Cytoplasmic Foci 2.73 3.08 3.20 0.50 0.44 2.88 -2.43 -4.95 -6.84 -1.54 3.50 -0.11 -0.37 -4.13 -0.25
Eisosomes -0.99 -2.57 8.81 8.76 5.60 3.68 -4.72 -5.69 -7.91 2.62 3.16 -5.29 2.39 -0.31 6.03
Endoplasmic Reticulum 3.08 6.86 6.25 7.71 -4.05 -21.59* 7.46 4.73 24.76 -3.84 -18.00 11.17 8.50 27.26 -5.50
Endosome 2.37 7.44 2.53 -0.38 -6.38 -2.27 5.60 1.69 4.36 -6.41 -1.54 7.35 -1.02 0.41 -7.51
Golgi 0.59 3.63 1.68 0.31 -3.21 0.31 3.51 -1.36 -1.42 -1.94 0.39 4.69 -1.76 -1.75 -3.53
Lipid Particles 2.65 3.05 2.98 2.10 -2.07 0.70 1.76 -1.24 -2.20 -3.14 2.49 3.73 2.55 0.17 -3.55
Mitochondria 8.72 9.19 8.01 -3.27 -4.87 2.53 -0.07 -11.98 -15.33 -8.26 6.27 4.28 -3.76 -14.08 -8.54
Mitotic Spindle 4.18 4.40 2.83 -5.18 -7.80 3.17 -0.16 -0.02 -3.11 0.15 5.03 2.07 -2.35 -9.34 -3.86
None -1.75 0.87 -0.84 1.36 -1.41 6.07 8.83 1.48 -3.54 -5.72 5.57 6.10 2.94 -3.67 -4.45
Nuclear Periphery 3.13 3.82 -6.14 -13.92 -15.17 -6.52 4.85 -5.05 1.15 -8.58 -5.62 5.67 -12.58 -6.47 -17.34
Nuclear Periphery Foci 1.62 5.85 0.13 -1.65 -7.93 1.41 5.20 -1.33 -2.57 -5.45 1.78 6.61 -4.18 -5.58 -9.39
Nucleolus -1.43 -1.78 3.13 5.45 4.40 16.32* 2.91 12.49 -3.19 3.06 15.56* 4.65 16.84 1.72 5.50
Nucleus -13.28 -14.21 -9.47 8.42 9.82 0.85 0.93 20.52 21.05 9.34 -3.36 -2.69 17.02 24.06 13.18
Peroxisomes 4.04 0.06 6.85 5.26 4.75 7.37 -2.57 5.05 -2.95 3.71 8.04 -2.93 9.41 2.36 5.97
Vacuole 3.28 6.60 3.88 0.51 -8.49 -9.72 1.01 -9.77 0.97 -8.05 -7.76 4.74 -6.65 2.29 -11.12
Vacuole Periphery 3.39 4.21 1.98 -3.49 -5.64 -1.00 8.38 -0.61 0.35 -7.35 0.20 7.32 -1.14 -1.26 -8.74