Standard name
Human Ortholog
Description Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Aac1p; has roles in maintenance of viability and in respiration; AAC3 has a paralog, PET9, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.07 – – 0.06 0.05 0 0 0 0.05
Bud 0 0 0.05 0 0 0.06 0 0.05 0.05 0 0.05 0.08 0 0 0 0 – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 – – 0 0 0 0 0 0
Cytoplasm 0.7 0.27 0.19 0.23 0.22 0.27 0.21 0.26 0.23 0.29 0.35 0.26 0.24 0.4 0.47 0.1 – – 0.28 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 – – 0 0 0 0 0 0
Endosome 0 0.06 0 0.12 0.05 0 0 0 0 0 0 0 0.09 0.21 0.25 0.07 – – 0 0.07 0.07 0.07 0.08 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0.3 – – 0 0 0 0 0 0
Mitochondria 0.26 0.56 0.76 0.69 0.75 0.76 0.84 0.81 0.82 0.75 0.75 0.79 0.3 0.25 0.07 0.07 – – 0.44 0.64 0.71 0.71 0.65 0.6
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0.06 0.09 0.1 – – 0 0 0.06 0 0.05 0
SpindlePole 0 0 0.07 0.09 0.05 0.09 0 0.08 0.06 0.07 0 0.08 0.06 0 0 0 – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0.15 0 0 0.06 0 0 0 0 0 0 0 0.19 0.18 0.18 0.23 – – 0 0.06 0 0.06 0 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 0 0 0 8 1 2 2 – – 5 28 8 3 9 15
Bud 0 6 17 11 13 22 22 23 18 14 24 43 1 4 1 1 – – 1 7 2 1 2 6
Bud Neck 0 0 1 0 2 1 4 0 0 1 1 3 1 2 0 0 – – 0 5 2 1 7 5
Bud Site 1 0 5 3 2 9 13 7 9 11 7 9 0 0 0 0 – – – – – – – –
Cell Periphery 1 16 6 7 1 3 1 2 2 0 1 3 6 4 4 3 – – 0 2 1 0 0 1
Cytoplasm 62 114 63 64 94 104 108 116 77 117 165 145 39 69 109 3 – – 28 21 11 3 6 4
Endoplasmic Reticulum 1 14 3 1 3 2 1 1 0 2 1 2 15 7 6 1 – – 0 6 4 3 3 2
Endosome 1 23 6 33 22 6 1 0 1 1 0 0 15 36 57 2 – – 3 42 27 18 19 22
Golgi 0 4 0 0 0 0 0 0 0 0 0 0 12 1 2 9 – – 0 10 3 3 5 12
Mitochondria 23 234 254 195 318 294 426 356 275 303 358 437 48 43 17 2 – – 44 365 273 187 165 183
Nucleus 2 3 0 0 5 8 9 7 9 8 5 4 2 0 2 0 – – 0 1 0 1 0 0
Nuclear Periphery 0 1 2 1 0 1 4 1 0 3 2 0 0 0 0 0 – – 0 3 1 2 2 3
Nucleolus 4 0 3 2 1 3 3 2 1 0 2 0 0 0 2 1 – – 0 3 1 1 0 1
Peroxisomes 1 17 14 22 10 4 5 4 0 1 0 4 1 10 21 3 – – 0 11 22 9 11 11
SpindlePole 4 15 24 25 21 36 22 33 20 29 20 44 9 6 5 1 – – 2 9 4 5 4 5
Vac/Vac Membrane 3 62 11 7 24 14 9 6 4 8 10 1 31 32 42 7 – – 2 36 6 15 11 24
Unique Cell Count 89 418 336 283 426 386 509 438 337 405 475 551 161 174 231 30 100 569 383 264 255 305
Labelled Cell Count 103 510 409 371 516 507 628 558 416 498 596 695 188 215 270 35 100 569 383 264 255 305


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.0 4.6 3.5 3.7 3.4 3.4 2.8 2.8 2.7 2.6 2.4 2.5 5.9 5.4 5.7 8.4 – – 6.0 6.4 6.3
Std Deviation (1e-4) 4.1 0.9 0.8 1.2 1.1 1.2 1.1 1.0 0.8 0.8 0.6 0.7 0.9 0.9 1.4 1.9 – – 1.2 4.8 1.3
Intensity Change (Log2) – – – 0.08 -0.03 -0.07 -0.33 -0.35 -0.4 -0.43 -0.57 -0.47 0.75 0.62 0.7 1.26 – – 0.77 0.87 0.84


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.2683 0.0307 -0.131 -0.0703 -0.3778 0.0609 2.3885 2.0477 2.0966 2.4274 1.5822 2.1733 -0.8934 -0.6121 -0.6813 -0.5941 -0.7108 -0.4879
Actin 0.0073 0.0034 0.0044 0.0026 0.0086 0.0042 0.0728 0.0137 0.0338 0.0358 0.0462 0.0095 0.0141 0.0057 0.0192 0.0058 0.0008 0.0077
Bud 0.0014 0.0035 0.0039 0.0013 0.0009 0.0009 0.0046 0.0097 0.0087 0.0145 0.0022 0.002 0.0018 0.0053 0.0019 0.0029 0.0012 0.0015
Bud Neck 0.0032 0.0044 0.0042 0.0009 0.0011 0.0057 0.0071 0.0012 0.0018 0.0028 0.0011 0.0112 0.0025 0.0013 0.0027 0.0031 0.0004 0.0093
Bud Periphery 0.0047 0.006 0.0144 0.005 0.0042 0.0038 0.0165 0.0175 0.0212 0.052 0.0078 0.0099 0.0075 0.0072 0.0065 0.0118 0.0035 0.0046
Bud Site 0.0077 0.032 0.0092 0.0012 0.0006 0.0015 0.0327 0.0142 0.0165 0.0184 0.0031 0.0023 0.0072 0.0463 0.0064 0.0024 0.0007 0.0015
Cell Periphery 0.0018 0.0011 0.0014 0.0007 0.0011 0.0008 0.0041 0.0019 0.0061 0.0045 0.0029 0.0018 0.0022 0.0023 0.0021 0.0026 0.0007 0.001
Cytoplasm 0.0842 0.0736 0.069 0.063 0.0779 0.0901 0.0791 0.114 0.0933 0.1016 0.1217 0.0691 0.0624 0.0912 0.066 0.1324 0.1042 0.0923
Cytoplasmic Foci 0.1073 0.0886 0.1047 0.076 0.135 0.0937 0.0872 0.1076 0.1053 0.1249 0.0673 0.1136 0.1366 0.1188 0.0858 0.1001 0.1142 0.1301
Eisosomes 0.0013 0.0006 0.001 0.0006 0.0006 0.0009 0.0017 0.0008 0.0024 0.0008 0.0008 0.0006 0.0037 0.0008 0.0013 0.0006 0.0006 0.0006
Endoplasmic Reticulum 0.0092 0.0035 0.0028 0.0018 0.0038 0.0054 0.0082 0.0085 0.0044 0.004 0.0025 0.0039 0.0076 0.0044 0.0064 0.0028 0.0038 0.0028
Endosome 0.0959 0.1031 0.055 0.0585 0.1734 0.0821 0.1382 0.1592 0.0891 0.1183 0.0883 0.0907 0.1189 0.1155 0.089 0.0835 0.124 0.0785
Golgi 0.0136 0.0323 0.0196 0.0246 0.0203 0.0158 0.0531 0.06 0.043 0.0356 0.0384 0.0366 0.0355 0.0428 0.0429 0.0204 0.0231 0.0225
Lipid Particles 0.0257 0.0132 0.0133 0.0063 0.0296 0.0238 0.0282 0.0166 0.0164 0.0083 0.0062 0.0207 0.0488 0.0156 0.0242 0.0091 0.03 0.0294
Mitochondria 0.102 0.1848 0.2464 0.2766 0.085 0.2163 0.1728 0.2685 0.2463 0.2266 0.2633 0.2701 0.2059 0.1745 0.3113 0.2809 0.2714 0.3043
None 0.4514 0.3724 0.3918 0.4657 0.3514 0.3961 0.2248 0.1501 0.2453 0.1104 0.2907 0.2783 0.2652 0.2979 0.275 0.2278 0.2523 0.2377
Nuclear Periphery 0.0073 0.0036 0.0016 0.0005 0.0146 0.0013 0.004 0.0031 0.0012 0.0024 0.001 0.0037 0.0025 0.0014 0.0051 0.0012 0.0017 0.0014
Nucleolus 0.0065 0.0022 0.0022 0.0007 0.003 0.0017 0.0011 0.0013 0.001 0.0016 0.0006 0.0008 0.0029 0.001 0.0013 0.0011 0.0013 0.0013
Nucleus 0.0051 0.0033 0.0026 0.0008 0.0035 0.0016 0.0017 0.0031 0.0016 0.0045 0.001 0.0015 0.0028 0.0016 0.0016 0.0025 0.0014 0.0018
Peroxisomes 0.0342 0.0485 0.0354 0.0072 0.0482 0.0381 0.0384 0.0186 0.0384 0.0844 0.0436 0.0574 0.0478 0.0509 0.0377 0.0698 0.0422 0.0545
Punctate Nuclear 0.0074 0.0022 0.0017 0.0007 0.0201 0.0019 0.0032 0.0008 0.0015 0.0014 0.0009 0.0028 0.003 0.0012 0.0015 0.0013 0.0029 0.0028
Vacuole 0.0178 0.0132 0.011 0.0031 0.0112 0.0103 0.016 0.0239 0.0168 0.0382 0.007 0.0095 0.0171 0.0113 0.0081 0.0316 0.0131 0.0105
Vacuole Periphery 0.0049 0.0044 0.0045 0.0022 0.0059 0.004 0.0046 0.0056 0.006 0.0088 0.0035 0.0041 0.0039 0.0031 0.0038 0.0063 0.0065 0.0039

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.927 21.3501 11.882 11.3185 17.8052 9.548 14.3015 14.5698 11.5981 16.1893
Translational Efficiency 1.4059 1.6246 1.5096 1.2011 1.0079 1.6748 1.622 1.2347 1.4403 1.0493

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1876 2724 1070 2612 1525 2010 1743 1835 3401 4734 2813 4447

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 696.61 809.08 776.42 891.57 674.84 724.09 831.53 869.69 686.85 772.99 810.57 882.54
Standard Deviation 82.31 106.31 107.11 121.16 104.03 96.47 100.12 123.48 93.31 110.54 106.26 122.60
Intensity Change Log 2 — 0.215931 0.156486 0.355997 — 0.101624 0.301223 0.365956 — 0.160816 0.229521 0.360906

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000534 0.000685 0.002817 0.009837 0.000423 0.000729 0.003943 0.007155 0.000485 0.000704 0.003515 0.008730
Bud Neck 0.016172 0.012951 0.028668 0.055992 0.021621 0.012737 0.054658 0.049274 0.018616 0.012860 0.044772 0.053220
Bud Site 0.014844 0.022012 0.052771 0.152835* 0.027390 0.013203 0.111064* 0.142569* 0.020470 0.018272 0.088891 0.148599*
Cell Periphery 0.000305 0.000218 0.000180 0.000277 0.000684 0.000349 0.000251 0.000359 0.000475 0.000274 0.000224 0.000311
Cytoplasm 0.028838 0.016244 0.205177* 0.213729* 0.018418 0.015143 0.149074* 0.196079* 0.024165 0.015776 0.170414* 0.206446*
Cytoplasmic Foci 0.080952 0.066603 0.126623 0.075504 0.053709 0.073662 0.088823 0.117520 0.068737 0.069600 0.103201 0.092842
Eisosomes 0.000150 0.000077 0.000103 0.000125 0.000115 0.000112 0.000115 0.000173 0.000134 0.000092 0.000111 0.000145
Endoplasmic Reticulum 0.001431 0.000537 0.008056 0.001994 0.001072 0.000636 0.004840 0.001921 0.001270 0.000579 0.006063 0.001964
Endosome 0.071202 0.073830 0.040545 0.025031 0.074003 0.102721 0.038090 0.043542 0.072458 0.086097 0.039024 0.032669
Golgi 0.073721 0.100905 0.023348 0.024729 0.082111 0.118422* 0.022719 0.027614 0.077483 0.108343 0.022958 0.025920
Lipid Particles 0.008443 0.002791 0.006985 0.001454 0.006988 0.010116 0.003325 0.002586 0.007791 0.005901 0.004717 0.001921
Mitochondria 0.619145* 0.590123* 0.054779 0.114506* 0.629219* 0.549934* 0.136385* 0.090669 0.623662* 0.573060* 0.105344* 0.104670*
Mitotic Spindle 0.006045 0.027899 0.087850 0.095138 0.005519 0.011370 0.105938* 0.057319 0.005809 0.020881 0.099058 0.079532
None 0.000579 0.000522 0.002453 0.000975 0.004269 0.001072 0.001382 0.000848 0.002234 0.000756 0.001789 0.000923
Nuclear Periphery 0.000237 0.000147 0.002959 0.000760 0.000206 0.000207 0.001244 0.000398 0.000223 0.000173 0.001897 0.000610
Nuclear Periphery Foci 0.000226 0.000145 0.002980 0.000321 0.000357 0.000363 0.000885 0.000431 0.000285 0.000238 0.001682 0.000367
Nucleolus 0.000536 0.000626 0.005308 0.000841 0.002109 0.000946 0.003027 0.000950 0.001242 0.000762 0.003894 0.000886
Nucleus 0.003148 0.003338 0.169445* 0.032243 0.005059 0.003586 0.090719 0.035131 0.004005 0.003443 0.120665 0.033435
Peroxisomes 0.020655 0.024031 0.027811 0.063692 0.016165 0.026788 0.051165 0.078609 0.018641 0.025201 0.042282 0.069847
Vacuole 0.031750 0.037573 0.133471 0.123755 0.026014 0.035653 0.120258 0.140803 0.029178 0.036758 0.125284 0.130790
Vacuole Periphery 0.021086 0.018741 0.017670 0.006261 0.024548 0.022250 0.012096 0.006050 0.022638 0.020231 0.014216 0.006174

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.25 -5.43 -11.82 -11.59 -6.84 -1.20 -9.72 -11.59 -10.23 -3.97 -1.71 -11.04 -15.94 -15.16 -7.68
Bud Neck 1.89 -5.12 -15.63 -19.07 -8.64 3.84 -10.05 -7.98 -13.63 3.00 4.13 -12.16 -16.78 -23.39 -1.97
Bud Site -4.63 -11.09 -29.90* -27.64* -16.03 5.88 -17.24 -19.22 -24.21 -3.11 1.55 -21.56 -34.60 -36.79* -10.54
Cell Periphery 2.73 4.49 2.54 -0.51 -2.71 3.89 5.64 4.39 0.67 -2.29 4.76 6.63 5.13 0.32 -2.68
Cytoplasm 5.34 -23.18 -39.74 -44.54 -3.39 1.42 -25.31 -32.21 -33.56 -7.31 5.03 -33.71 -51.29 -55.65 -9.07
Cytoplasmic Foci 4.06 -7.63 3.64 -0.51 10.89 -5.22 -8.20 -12.86 -8.41 -4.97 -0.25 -9.68 -5.87 -6.24 4.81
Eisosomes 7.49 3.93 3.49 -7.87 -0.89 0.22 -0.14 -3.27 -4.74 -5.37 5.18 2.54 0.09 -8.13 -4.08
Endoplasmic Reticulum 2.85 -12.01 -2.06 -9.35 12.03 1.82 -10.93 -3.66 -7.22 8.94 3.40 -15.50 -3.72 -11.79 14.73
Endosome -0.72 9.76 17.36 21.00 7.67 -6.44 11.13 10.24 17.90 -0.80 -4.85 15.08 19.86 27.40 5.58
Golgi -6.49 15.02 17.28 25.77 0.90 -6.82 15.82 15.08 22.95 -0.94 -9.32 22.12 22.95 34.47 0.14
Lipid Particles 5.25 1.13 6.85 2.23 8.89 -2.14 3.50 4.48 6.64 3.15 2.15 3.87 8.20 6.87 9.86
Mitochondria 3.01 68.02* 64.75* 69.29* -7.74 6.87 50.16* 58.22* 56.04* 7.35 6.79 79.00* 86.96* 88.89* 2.55
Mitotic Spindle -12.54 -14.03 -21.98 -14.70 -0.11 -5.20 -20.42* -15.05 -12.62 8.84 -13.54 -24.67 -26.53 -19.10 5.51
None 0.60 -9.74 -3.40 -3.98 5.79 3.21 2.97 3.40 0.41 2.26 3.29 1.13 2.67 -1.96 4.97
Nuclear Periphery 1.46 -4.42 -6.74 -7.59 2.93 -0.01 -9.69 -5.61 -6.67 6.78 1.07 -7.26 -8.30 -9.29 4.31
Nuclear Periphery Foci 1.35 -8.60 -2.83 -6.92 8.16 0.04 -3.68 -2.00 -1.05 2.71 0.60 -9.02 -3.31 -2.83 8.12
Nucleolus -0.61 -9.75 -4.16 -2.98 8.76 2.41 -2.36 2.52 -0.02 5.26 2.13 -7.33 1.14 -2.13 8.92
Nucleus -0.32 -24.15 -23.38 -23.07 16.67 1.27 -21.46 -17.13 -17.97 10.37 0.78 -31.93 -28.78 -29.22 18.72
Peroxisomes -1.63 -2.45 -11.07 -10.03 -8.04 -4.97 -10.85 -14.56 -10.97 -4.37 -4.41 -10.28 -18.00 -14.77 -6.72
Vacuole -2.58 -19.91 -32.18 -31.16 -4.98 -4.02 -24.62 -29.41 -27.30 -8.08 -4.58 -31.33 -43.70 -41.43 -9.53
Vacuole Periphery 1.88 2.95 15.46 15.72 6.75 1.30 8.39 13.14 15.90 6.48 2.30 8.79 20.03 22.31 9.39
Standard name
Human Ortholog
Description Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Aac1p; has roles in maintenance of viability and in respiration; AAC3 has a paralog, PET9, that arose from the whole genome duplication
Localization
Cell Percentages mitochondrion (96%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Aac3

Aac3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aac3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available