Standard name
Human Ortholog
Description Thiol-specific peroxiredoxin; reduces hydroperoxides to protect against oxidative damage; function in vivo requires covalent conjugation to Urm1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.43 0.23 0.29 0.28 0.55 0.13 0.43 – 0.38 0.36 0.21 0.31 0.4 0.67 0.72 0.47 0.55 0.26 0.3 0.1 0.05 0 0 0.07
Cytoplasm 0.67 0.83 0.81 0.84 0.57 0.9 0.68 – 0.7 0.71 0.83 0.79 0.67 0.45 0.39 0.66 0.57 0.79 0.67 0.87 0.91 0.93 0.93 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 3 0 0 0 6 1 – 0 1 1 0 0 0 0 0 0 0 0 0 0 1 0 2
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 57 24 43 56 112 22 123 – 99 115 47 71 73 218 194 71 63 45 61 18 9 6 20 29
Cytoplasm 89 87 120 168 117 156 194 – 183 226 185 179 122 148 105 100 65 139 137 153 158 362 440 371
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 6 0 0 0 0 0 3 6 6 1 1 0 6 4 4
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 1 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Nucleolus 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 2 1 2 2 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 1
Unique Cell Count 132 105 149 199 204 173 286 260 317 222 228 181 326 269 152 115 175 205 176 173 388 474 419
Labelled Cell Count 146 114 166 225 231 187 318 282 351 235 250 195 366 299 174 134 190 205 176 173 388 474 419


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 638.4 601.3 545.4 528.8 545.6 429.9 506.7 – 459.2 433.8 418.9 419.6 639.4 696.6 739.2 669.5 664.2 616.9 509.6 550.8 559.7
Std Deviation (1e-4) 117.3 88.7 115.1 100.8 103.3 144.3 113.6 – 105.8 105.7 121.6 110.8 116.0 141.5 150.0 121.9 101.3 115.6 110.0 107.6 136.7
Intensity Change (Log2) – – – -0.04 0.0 -0.34 -0.11 – -0.25 -0.33 -0.38 -0.38 0.23 0.35 0.44 0.3 0.28 0.18 -0.1 0.01 0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.1 4.9 -3.6 2.9 – 1.9 1.6 -1.7 0.5 2.2 7.7 8.5 3.2 4.3 -0.6
Cytoplasm 0.9 -4.6 2.5 -2.8 – -2.3 -2.1 0.7 -0.5 -2.7 -7.2 -8.2 -2.9 -4.2 -0.2
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 656.1342 871.7217 790.4047 642.3939 662.8164 712.1812 881.9373 1100.9455 994.5633 890.396 922.1276 917.4167 771.7744 982.6252 803.426 699.5345 676.4864 752.0451
Actin 0.017 0.0001 0.0001 0.0007 0.0001 0.0002 0.0002 0.0001 0.0001 0.0003 0.0003 0.0001 0.0007 0.0001 0.0005 0.0026 0.0168 0.0004
Bud 0.0008 0.0002 0.0001 0.0003 0.0001 0.0001 0.0003 0.0002 0 0.0004 0.0006 0.0001 0.0016 0.0003 0.0006 0.0004 0.0006 0.002
Bud Neck 0.0003 0.0001 0.0001 0.0003 0.0001 0.0003 0.0001 0 0.0001 0.0001 0.0003 0.0003 0.0002 0 0.0001 0.0002 0.0003 0.0004
Bud Periphery 0.0013 0 0 0 0 0 0.0001 0 0 0 0.0001 0 0.0003 0 0.0001 0.0003 0.0004 0.0007
Bud Site 0.0013 0 0 0 0.0001 0 0.0001 0 0 0 0 0 0.0005 0 0.0003 0 0.0017 0.0002
Cell Periphery 0.0008 0.0001 0.0001 0.0004 0.0008 0 0.0004 0 0.0001 0 0.0001 0 0.0002 0 0.0003 0 0.0011 0.0005
Cytoplasm 0.7912 0.947 0.9398 0.7525 0.8338 0.9285 0.894 0.9628 0.962 0.8848 0.8045 0.9295 0.8614 0.9844 0.8377 0.8519 0.6741 0.8629
Cytoplasmic Foci 0.0073 0 0.0001 0.0003 0.0074 0.0001 0.0012 0 0.0001 0.0001 0.0001 0.0001 0.0077 0 0.0239 0.0001 0.0599 0.0019
Eisosomes 0.0015 0.0001 0 0 0.0021 0 0.0002 0 0 0 0 0 0.0003 0 0.0006 0 0.0056 0.0001
Endoplasmic Reticulum 0.0007 0 0 0.0001 0 0.0001 0.0001 0 0 0 0.0001 0.0001 0.0007 0 0.0001 0.0015 0.002 0.0003
Endosome 0.0048 0 0 0 0 0 0 0 0 0 0 0 0.0033 0 0.0001 0.0011 0.0083 0.0001
Golgi 0.0027 0 0 0 0 0 0 0 0 0 0 0 0.0002 0 0 0 0.0011 0
Lipid Particles 0.0054 0 0 0 0.0018 0 0.0003 0 0 0 0 0 0.0005 0 0.0008 0 0.0048 0.0001
Mitochondria 0.0296 0.014 0.0008 0.0039 0.0182 0.0003 0.0034 0.0026 0.0004 0.0011 0.0012 0.0003 0.0119 0.0004 0.0186 0.0019 0.0303 0.0088
None 0.012 0.0004 0.0013 0.0026 0.0045 0.0005 0.0043 0.0004 0.0005 0.0006 0.0011 0.0004 0.0152 0.0003 0.0294 0.0004 0.0247 0.0085
Nuclear Periphery 0.009 0.0029 0.0039 0.0245 0.0061 0.0046 0.0089 0.0018 0.0045 0.0108 0.0128 0.0061 0.0136 0.0007 0.0022 0.0764 0.0455 0.005
Nucleolus 0.0412 0.0008 0.0004 0.0035 0.0716 0.0011 0.0132 0.0003 0.0001 0.0007 0.001 0.0001 0.0033 0 0.0341 0.0001 0.0564 0.0171
Nucleus 0.0608 0.0339 0.0528 0.2077 0.0499 0.0636 0.0717 0.0313 0.0319 0.1007 0.1765 0.0624 0.0743 0.0137 0.0404 0.0588 0.0534 0.0872
Peroxisomes 0.0076 0 0 0.0001 0.0009 0 0.0001 0 0 0 0 0 0.0005 0 0.0009 0 0.0011 0.0002
Punctate Nuclear 0.0026 0 0.0001 0.0007 0.0003 0.0004 0.0005 0 0.0001 0.0002 0.0003 0.0002 0.0018 0 0.0079 0.0003 0.0046 0.0004
Vacuole 0.001 0.0001 0.0001 0.001 0.0009 0.0001 0.0005 0.0001 0.0001 0.0001 0.0005 0.0001 0.0011 0 0.0007 0.0011 0.0023 0.002
Vacuole Periphery 0.0012 0.0001 0.0001 0.0014 0.0011 0.0001 0.0004 0.0001 0.0001 0.0002 0.0004 0.0001 0.0008 0 0.0006 0.0028 0.0049 0.0012

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2045.7659 2279.2534 3017.3311 3975.7939 2307.5692 2116.2838 3459.1739 3914.5281 4068.8375 3072.6547
Translational Efficiency 2.0317 2.3078 2.3738 1.5793 2.0177 3.2322 2.5386 2.1361 2.3689 2.3365

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
134 284 1420 109 1287 2334 308 1134 1421 2618 1728 1243

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 38978.84 33023.62 31853.90 47175.70 25825.19 33947.90 36810.46 42200.94 27065.58 33847.63 32737.36 42637.18
Standard Deviation 7030.29 7856.59 7077.80 8339.56 6720.01 7106.14 7135.19 8946.66 7767.75 7197.08 7337.51 9005.68
Intensity Change Log 2 — -0.239193 -0.291221 0.275353 — 0.394543 0.511337 0.708496 — 0.047464 0.083478 0.463814

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000072 0.000099 0.000057 0.000147 0.000044 0.000029 0.000091 0.000201 0.000046 0.000036 0.000063 0.000196
Bud Neck 0.000725 0.001378 0.000643 0.003013 0.000310 0.000196 0.002597 0.000867 0.000349 0.000325 0.000991 0.001055
Bud Site 0.000475 0.001417 0.000480 0.003270 0.000248 0.000288 0.000679 0.002128 0.000270 0.000410 0.000516 0.002228
Cell Periphery 0.071690 0.018808 0.007880 0.095018 0.003073 0.001312 0.019920 0.096182 0.009544 0.003210 0.010026 0.096080
Cytoplasm 0.632449* 0.682533* 0.643459* 0.265270* 0.644402* 0.746546* 0.545490* 0.409078* 0.643274* 0.739602* 0.625997* 0.396468*
Cytoplasmic Foci 0.005142 0.008901 0.004035 0.002000 0.004485 0.001136 0.012368 0.000863 0.004547 0.001979 0.005521 0.000962
Eisosomes 0.000011 0.000003 0.000001 0.000025 0.000002 0.000000 0.000008 0.000011 0.000002 0.000001 0.000003 0.000012
Endoplasmic Reticulum 0.000056 0.000078 0.000051 0.000037 0.000061 0.000036 0.000018 0.000348 0.000061 0.000041 0.000045 0.000320
Endosome 0.000025 0.000038 0.000016 0.000104 0.000018 0.000011 0.000025 0.000161 0.000019 0.000014 0.000018 0.000156
Golgi 0.002012 0.001140 0.000399 0.000701 0.000934 0.000192 0.000408 0.000151 0.001036 0.000295 0.000400 0.000199
Lipid Particles 0.009754 0.005218 0.001845 0.013107 0.000686 0.000346 0.005523 0.002582 0.001541 0.000875 0.002501 0.003505
Mitochondria 0.000133 0.000282 0.000198 0.000043 0.000274 0.000157 0.000123 0.000134 0.000261 0.000170 0.000184 0.000126
Mitotic Spindle 0.000056 0.000060 0.000131 0.010870 0.000094 0.000200 0.000021 0.000036 0.000090 0.000185 0.000112 0.000986
None 0.011284 0.014711 0.007728 0.000292 0.012690 0.014669 0.005585 0.002322 0.012558 0.014674 0.007346 0.002144
Nuclear Periphery 0.000658 0.000363 0.000331 0.000921 0.000243 0.000084 0.000669 0.000172 0.000283 0.000114 0.000391 0.000238
Nuclear Periphery Foci 0.002474 0.002381 0.000850 0.003276 0.000638 0.000344 0.001136 0.000673 0.000811 0.000565 0.000901 0.000902
Nucleolus 0.000067 0.000124 0.000045 0.000071 0.000047 0.000050 0.000069 0.000080 0.000049 0.000058 0.000049 0.000080
Nucleus 0.128498 0.191095* 0.297055* 0.026076 0.322143* 0.214881* 0.276419* 0.030860 0.303882* 0.212301* 0.293377* 0.030440
Peroxisomes 0.000004 0.000014 0.000003 0.000011 0.000002 0.000004 0.000003 0.000013 0.000002 0.000005 0.000003 0.000013
Vacuole 0.128296 0.069455 0.034310 0.574078* 0.006428 0.019244 0.128182 0.452413* 0.017920 0.024691 0.051042 0.463082*
Vacuole Periphery 0.006119 0.001901 0.000481 0.001669 0.003178 0.000273 0.000665 0.000725 0.003456 0.000450 0.000514 0.000807

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.58 0.55 -1.63 -0.91 -1.78 2.36 -7.26 -4.31 -4.60 -1.48 0.99 -6.13 -4.50 -4.63 -3.36
Bud Neck -2.04 -0.76 -5.94 -2.49 -5.81 0.95 -9.28 -5.99 -6.39 7.54 -1.34 -10.09 -7.53 -6.24 2.06
Bud Site -2.89 -0.05 -1.14 -0.74 -1.14 -1.64 -3.71 -4.11 -3.86 -1.59 -3.07 -3.18 -4.12 -3.64 -3.26
Cell Periphery 4.25 5.15 -0.10 -6.30 -7.82 3.27 -4.67 -18.38 -18.98 -14.96 4.69 0.60 -17.29 -19.69 -18.04
Cytoplasm -1.20 0.63 7.94 11.08 10.25 -12.13 10.01 15.68 26.58 1.79 -11.60 5.59 17.23 28.23 12.99
Cytoplasmic Foci -2.46 0.06 -1.01 0.64 -1.08 7.26 -7.16 5.92 0.16 8.26 3.92 -5.79 4.12 0.97 8.43
Eisosomes 1.20 1.68 -1.50 -2.83 -3.22 4.72 -7.44 -7.23 -8.54 0.74 3.05 -1.88 -6.68 -8.73 -6.17
Endoplasmic Reticulum -0.98 0.14 0.57 1.34 0.47 1.22 1.95 -1.03 -1.14 -1.20 1.06 0.69 -1.02 -1.12 -1.09
Endosome -1.98 1.31 -1.73 -1.12 -1.87 1.01 -3.69 -1.03 -1.08 -0.83 0.33 -1.09 -1.09 -1.11 -1.05
Golgi 1.79 3.22 2.43 1.16 -1.34 4.61 2.87 4.83 1.29 5.14 4.84 3.87 5.36 2.00 4.83
Lipid Particles 1.68 3.11 -1.12 -3.15 -4.81 1.03 -4.65 -5.83 -8.45 2.46 1.67 -2.53 -4.84 -7.76 -2.06
Mitochondria -1.32 -4.21 2.67 2.04 8.20 4.54 5.43 2.39 0.32 0.16 3.57 2.59 2.49 0.71 1.59
Mitotic Spindle -1.33 -1.93 -0.99 -0.99 -0.98 -0.94 2.04 1.60 1.33 -0.29 -0.96 -0.55 -0.89 -0.62 -0.85
None -0.54 0.72 2.32 5.52 17.15 -1.07 7.51 8.41 8.70 1.93 -1.18 5.59 8.66 9.53 5.95
Nuclear Periphery 1.64 1.50 -1.28 -2.17 -2.07 4.32 -7.65 1.74 -3.99 8.58 4.43 -6.19 0.68 -3.81 7.06
Nuclear Periphery Foci -0.75 3.22 -0.71 -0.26 -2.07 1.69 -4.67 -0.63 -2.53 4.17 0.77 -2.20 -0.85 -1.61 1.03
Nucleolus -1.77 1.31 -1.00 1.37 -2.87 -1.11 -5.20 -4.24 -3.76 1.01 -1.90 -1.75 -4.33 -2.75 -3.58
Nucleus -3.20 -10.33 3.02 6.91 15.58 13.25 -0.01 37.25 30.92 20.60 11.76 -2.11 35.87 31.51 40.28
Peroxisomes -2.27 0.14 -2.32 0.91 -2.37 -3.76 -3.94 -6.17 -4.36 -3.95 -4.39 -2.41 -6.42 -3.83 -4.93
Vacuole 2.80 4.72 -9.99 -13.27 -14.86 -6.35 -9.92 -43.22 -41.14 -18.66 -2.63 -8.05 -43.53 -42.98 -38.41
Vacuole Periphery 0.98 1.28 1.02 0.26 -4.37 3.01 2.73 2.55 -5.74 -1.66 3.12 3.07 2.76 -3.77 -3.70
Standard name
Human Ortholog
Description Thiol-specific peroxiredoxin; reduces hydroperoxides to protect against oxidative damage; function in vivo requires covalent conjugation to Urm1p
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Ahp1

Ahp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ahp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available