Standard name
Human Ortholog
Description Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.05 0.07 0.05 0 0.06 0.05 0 0 0 0 0.08 0.07 0 0 0 0 0 0
Bud Neck 0 0 0.08 0.05 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0.06 0 0 0 0 0 0
Cytoplasm 0.3 0.34 0.8 0.71 0.66 0.52 0.37 0.41 0.31 0.44 0.33 0.38 0.87 0.88 0.9 0.64 0.37 0.49 0.08 0 0.08 0.13 0.16 0.15
Endoplasmic Reticulum 0 0.12 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0.08 0.15 0.11 0.08 0.07 0.06 0.07 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.8 0.43 0.05 0.15 0.18 0.45 0.67 0.52 0.75 0.62 0.83 0.65 0 0 0 0.08 0.08 0.09 0.6 0.7 0.68 0.58 0.57 0.55
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0
SpindlePole 0 0 0.08 0 0.09 0.07 0.08 0.12 0.06 0.07 0 0.06 0 0 0 0.09 0.16 0.12 0 0 0 0 0.05 0
Vac/Vac Membrane 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.15 0 0.05 0.07 0 0.07 0.06 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 7 5 10 0 0 0 0 1 0 0 0 9 5 2 0 7 3 4 3 5 4 11 12
Bud 2 2 1 2 6 3 17 27 11 13 12 16 2 4 0 2 12 11 1 0 1 2 5 7
Bud Neck 2 0 13 8 14 8 5 20 2 4 0 8 12 5 5 3 3 2 2 3 1 5 5 10
Bud Site 1 0 1 0 3 2 7 6 3 8 0 1 0 0 0 0 0 2 – – – – – –
Cell Periphery 0 11 3 5 1 1 2 2 0 0 0 3 6 9 4 15 8 10 0 1 6 1 4 3
Cytoplasm 39 44 132 104 209 152 133 161 75 147 70 122 291 353 395 138 55 82 12 7 21 33 54 47
Endoplasmic Reticulum 2 15 5 1 1 0 0 0 3 1 0 1 6 2 3 17 8 8 3 1 4 1 3 4
Endosome 1 1 1 1 18 5 2 1 0 0 0 2 8 15 13 17 22 18 12 13 14 16 12 10
Golgi 0 0 0 2 0 0 0 0 0 0 0 0 0 0 1 2 1 3 0 1 1 0 0 1
Mitochondria 103 55 9 22 58 130 240 207 181 209 179 207 6 7 5 18 12 15 88 131 176 147 194 176
Nucleus 0 2 0 4 2 2 5 8 5 7 3 5 1 1 4 3 0 4 0 0 0 0 2 0
Nuclear Periphery 1 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 1 0 1
Nucleolus 1 0 1 0 0 1 0 1 0 0 1 0 0 0 0 1 0 1 1 0 0 0 0 0
Peroxisomes 0 0 0 1 7 1 0 0 0 1 1 0 0 8 3 1 9 10 1 2 3 2 1 2
SpindlePole 0 3 13 4 30 19 27 48 14 24 6 20 6 11 5 19 24 20 4 2 4 9 16 13
Vac/Vac Membrane 2 8 4 1 12 9 4 6 3 12 8 11 11 17 16 14 22 4 8 14 7 16 19 18
Unique Cell Count 129 128 166 146 316 290 358 396 242 337 215 320 336 403 439 215 147 167 149 189 259 255 341 321
Labelled Cell Count 154 148 188 165 361 334 442 487 299 426 280 396 358 437 456 250 184 193 149 189 259 255 341 321


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 4.7 4.8 4.7 4.7 3.6 3.4 4.0 3.2 3.4 3.2 3.6 4.9 5.2 5.2 6.4 6.1 5.7 4.8 5.1 5.6
Std Deviation (1e-4) 0.6 0.7 1.1 1.8 1.8 0.9 1.2 1.4 0.9 0.8 0.9 1.1 0.6 1.0 1.1 2.1 1.7 1.9 1.2 1.3 1.8
Intensity Change (Log2) – – – -0.02 -0.02 -0.42 -0.49 -0.27 -0.56 -0.51 -0.58 -0.4 0.03 0.11 0.12 0.43 0.35 0.25 0.02 0.08 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 –
Bud Neck – – – – – – – – – – – – 0 0 –
Bud Site – – – – – – – – – – – – 0 0 –
Cell Periphery – – – – – – – – – – – – 0 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 –
Endosome – – – – – – – – – – – – 0 0 –
Golgi – – – – – – – – – – – – 0 0 –
Mitochondria – – – – – – – – – – – – 0 0 –
Nuclear Periphery – – – – – – – – – – – – 0 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 –
Peroxisomes – – – – – – – – – – – – 0 0 –
SpindlePole – – – – – – – – – – – – 0 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 –
Cytoplasm – – – – – – – – – – – – 0 0 –
Nucleus – – – – – – – – – – – – 0 0 –
Vacuole – – – – – – – – – – – – 1.9 4.0 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.2383 -0.2327 -0.2899 -0.5372 0.4858 -0.478 0.1288 0.4715 0.3745 0.0311 -0.0517 0.5613 0.2921 0.3485 -0.0031 0.776 0.4739 0.1316
Actin 0.0353 0.0095 0.0124 0.0034 0.0658 0.0072 0.0129 0.0055 0.0042 0.0131 0.0098 0.0059 0.0444 0.0147 0.0167 0.0225 0.0045 0.0057
Bud 0.0018 0.004 0.003 0.0026 0.0035 0.0016 0.0051 0.0064 0.0101 0.0096 0.0053 0.0064 0.0102 0.0032 0.0045 0.0061 0.0267 0.0017
Bud Neck 0.0055 0.0019 0.0027 0.0008 0.0038 0.0075 0.0054 0.003 0.0041 0.0021 0.0015 0.0154 0.0028 0.003 0.0055 0.0014 0.0015 0.011
Bud Periphery 0.0049 0.01 0.0087 0.0102 0.0098 0.0046 0.0114 0.0126 0.016 0.0296 0.0118 0.0091 0.0227 0.0077 0.0082 0.0232 0.0475 0.0039
Bud Site 0.0149 0.0474 0.0083 0.0012 0.0115 0.0007 0.0084 0.0721 0.0309 0.0056 0.0031 0.003 0.0098 0.0519 0.0387 0.0058 0.0085 0.0027
Cell Periphery 0.0037 0.0028 0.0023 0.0011 0.003 0.001 0.0013 0.0033 0.0024 0.0012 0.0004 0.0015 0.0049 0.0029 0.0032 0.001 0.0031 0.0013
Cytoplasm 0.0849 0.1572 0.1515 0.1327 0.0646 0.142 0.1507 0.1681 0.1634 0.1127 0.0954 0.1558 0.1205 0.2009 0.1972 0.1298 0.0892 0.1261
Cytoplasmic Foci 0.1328 0.0893 0.1283 0.1483 0.1821 0.1108 0.1618 0.1338 0.1478 0.0992 0.1666 0.1607 0.1014 0.1323 0.1076 0.1336 0.11 0.1096
Eisosomes 0.0025 0.0008 0.0006 0.0006 0.0018 0.0004 0.0004 0.0006 0.0004 0.0002 0.0002 0.0003 0.0014 0.0011 0.0007 0.0004 0.0002 0.0005
Endoplasmic Reticulum 0.009 0.0062 0.0087 0.0051 0.0051 0.007 0.0067 0.0048 0.0055 0.004 0.0022 0.0041 0.0152 0.0143 0.0075 0.0071 0.0021 0.0052
Endosome 0.1255 0.1208 0.1686 0.2026 0.187 0.1421 0.1444 0.1433 0.124 0.1373 0.2055 0.139 0.1984 0.1662 0.1385 0.2103 0.1417 0.149
Golgi 0.0476 0.0208 0.0235 0.0348 0.0342 0.0286 0.0249 0.0339 0.0267 0.024 0.0572 0.0298 0.0619 0.0549 0.0647 0.0318 0.0661 0.0603
Lipid Particles 0.0539 0.0112 0.0278 0.0201 0.0227 0.0167 0.0183 0.0107 0.0084 0.0068 0.0101 0.0154 0.0374 0.0087 0.0111 0.0118 0.0225 0.0271
Mitochondria 0.0835 0.1076 0.1496 0.2011 0.1137 0.154 0.1124 0.1397 0.1415 0.3101 0.1761 0.186 0.1109 0.1177 0.2032 0.1189 0.3167 0.2745
None 0.2796 0.3631 0.2266 0.1613 0.1577 0.3203 0.2732 0.2042 0.2481 0.1577 0.1758 0.1911 0.1655 0.1724 0.1434 0.1703 0.112 0.1772
Nuclear Periphery 0.0067 0.0023 0.0066 0.0021 0.016 0.004 0.0045 0.0017 0.0034 0.0015 0.0142 0.0023 0.0089 0.0021 0.0015 0.0248 0.0005 0.0008
Nucleolus 0.0027 0.0023 0.0018 0.0037 0.0037 0.0007 0.002 0.0034 0.003 0.0019 0.0012 0.0015 0.0017 0.001 0.0007 0.0009 0.0007 0.0003
Nucleus 0.027 0.0047 0.003 0.0037 0.0171 0.0021 0.0035 0.0053 0.0069 0.0052 0.0033 0.0033 0.0169 0.0017 0.0016 0.0134 0.0014 0.001
Peroxisomes 0.049 0.015 0.0292 0.037 0.0467 0.0307 0.0254 0.0201 0.0285 0.052 0.0351 0.0487 0.0388 0.0188 0.0271 0.042 0.0193 0.0295
Punctate Nuclear 0.0049 0.0021 0.0067 0.003 0.0093 0.0023 0.0075 0.0051 0.009 0.0022 0.0075 0.0088 0.0034 0.0014 0.001 0.0043 0.0006 0.001
Vacuole 0.0187 0.0174 0.0232 0.0178 0.0282 0.0109 0.0161 0.0181 0.0121 0.0179 0.0118 0.0086 0.0182 0.0188 0.0131 0.0228 0.02 0.0076
Vacuole Periphery 0.0056 0.0036 0.0068 0.0066 0.0124 0.0048 0.0037 0.0044 0.0035 0.0062 0.0059 0.0035 0.0048 0.0045 0.0045 0.0175 0.0055 0.004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.5668 7.1477 4.5462 10.4673 5.9239 17.1241 14.9702 14.9816 15.0949 14.3316
Translational Efficiency 1.2329 1.339 1.6975 1.0497 1.6344 0.8685 0.9867 1.0237 1.2389 1.115

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
78 1044 151 1602 1207 1449 98 1598 1285 2493 249 3200

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 721.54 696.32 909.16 805.67 662.07 733.01 961.09 837.01 665.68 717.65 929.60 821.32
Standard Deviation 81.39 92.08 90.18 117.18 76.22 92.87 110.18 117.47 77.85 94.29 101.75 118.36
Intensity Change Log 2 — -0.051329 0.333455 0.159110 — 0.146849 0.537688 0.338261 — 0.046902 0.434794 0.247614

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000690 0.002207 0.001941 0.008385 0.000638 0.002015 0.002352 0.003507 0.000641 0.002096 0.002103 0.005949
Bud Neck 0.047322 0.040763 0.009801 0.031085 0.041577 0.051936 0.013357 0.015874 0.041926 0.047257 0.011201 0.023489
Bud Site 0.007307 0.042076 0.010163 0.089128 0.014393 0.040992 0.021680 0.055227 0.013963 0.041446 0.014696 0.072199
Cell Periphery 0.000492 0.000339 0.000520 0.000390 0.000308 0.000356 0.000462 0.000293 0.000319 0.000349 0.000497 0.000342
Cytoplasm 0.145612* 0.080045 0.226352* 0.343232* 0.186679* 0.097681 0.231004* 0.427819* 0.184187* 0.090295 0.228183* 0.385473*
Cytoplasmic Foci 0.340289* 0.235056* 0.126411 0.090492 0.378712* 0.269345* 0.138553 0.088645 0.376380* 0.254986* 0.131190 0.089570
Eisosomes 0.000468 0.000242 0.000248 0.000187 0.000328 0.000257 0.000257 0.000139 0.000336 0.000251 0.000251 0.000163
Endoplasmic Reticulum 0.002110 0.002812 0.014092 0.003306 0.001523 0.001681 0.011214 0.002880 0.001559 0.002155 0.012959 0.003094
Endosome 0.082765 0.073466 0.035077 0.016727 0.056809 0.067406 0.028888 0.014098 0.058385 0.069944 0.032641 0.015415
Golgi 0.016037 0.064959 0.001913 0.010748 0.036384 0.054915 0.001641 0.006278 0.035149 0.059121 0.001806 0.008516
Lipid Particles 0.041463 0.012844 0.023084 0.003069 0.029546 0.012321 0.010175 0.002765 0.030269 0.012540 0.018003 0.002917
Mitochondria 0.094784 0.247571* 0.002274 0.011433 0.096521 0.190254* 0.000654 0.003133 0.096416 0.214257* 0.001637 0.007288
Mitotic Spindle 0.000717 0.021577 0.004367 0.029983 0.005231 0.018626 0.004110 0.023594 0.004957 0.019862 0.004266 0.026793
None 0.005534 0.002025 0.001936 0.001233 0.002428 0.001417 0.003312 0.002572 0.002617 0.001672 0.002477 0.001902
Nuclear Periphery 0.000525 0.000367 0.005349 0.000933 0.000479 0.000362 0.002097 0.000855 0.000482 0.000364 0.004069 0.000894
Nuclear Periphery Foci 0.000731 0.000424 0.011682 0.001010 0.000477 0.000284 0.005452 0.001394 0.000492 0.000343 0.009230 0.001202
Nucleolus 0.001632 0.002242 0.005849 0.000850 0.001780 0.001694 0.001590 0.000617 0.001771 0.001923 0.004173 0.000734
Nucleus 0.073212 0.027459 0.344499* 0.121084 0.051811 0.028638 0.262062* 0.142731* 0.053110 0.028144 0.312054* 0.131894*
Peroxisomes 0.087109 0.084010 0.009943 0.034088 0.042858 0.085078 0.023096 0.016867 0.045544 0.084631 0.015120 0.025488
Vacuole 0.043659 0.047848 0.161307 0.197712 0.044524 0.066100 0.237321* 0.186057 0.044471 0.058457 0.191224 0.191891
Vacuole Periphery 0.007543 0.011666 0.003191 0.004924 0.006993 0.008643 0.000719 0.004655 0.007027 0.009909 0.002218 0.004789

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.49 -3.57 -6.00 -4.23 -4.55 -6.40 -3.44 -3.46 -0.64 -0.38 -8.13 -5.57 -7.06 -3.99 -3.99
Bud Neck 0.46 2.97 1.62 4.22 -7.59 -2.65 6.59 10.00 13.16 -0.51 -1.60 9.94 7.87 12.17 -4.85
Bud Site -10.78 -1.54 -14.87 -4.83 -14.28 -9.81 -1.12 -8.20 0.63 -4.03 -12.57 0.15 -14.88 -3.47 -13.20
Cell Periphery 1.27 -0.32 1.65 0.93 3.98 -0.59 -2.15 2.13 1.95 4.24 -0.52 -3.37 1.51 2.00 5.88
Cytoplasm 2.97 -2.70 -8.30 -30.80 -6.42 11.79 -0.66 -21.27 -35.50 -8.14 13.82 -1.85 -22.36 -46.43 -10.61
Cytoplasmic Foci 4.62 9.03 11.81 24.17 2.83 12.76 15.68 43.68 31.73 2.69 16.23 22.97 46.63 39.86 3.95
Eisosomes 2.05 2.00 2.84 4.72 3.82 3.87 2.09 12.91 9.12 5.27 4.91 3.78 12.73 9.80 6.51
Endoplasmic Reticulum -0.81 -8.03 -1.67 -1.26 8.11 -0.50 -5.84 -4.07 -2.72 5.18 -1.98 -10.84 -6.64 -3.11 9.54
Endosome 0.49 2.64 3.69 17.22 4.39 -2.64 6.38 15.98 20.22 3.94 -3.15 7.04 16.05 26.47 5.88
Golgi -10.76 5.11 3.15 15.46 -3.96 -5.18 15.43 13.72 18.08 -2.60 -7.80 15.72 13.45 23.53 -4.65
Lipid Particles 2.28 1.54 3.10 8.48 6.23 7.07 7.71 12.47 9.41 6.13 7.63 4.60 12.65 12.56 7.56
Mitochondria -6.54 4.24 3.97 26.56* -4.56 -10.88 18.50 18.19 27.69 -4.75 -15.74 18.78 18.04 38.03* -5.09
Mitotic Spindle -9.70 -3.25 -9.95 -0.77 -7.85 -5.52 -0.60 -5.67 -0.35 -3.50 -7.73 -0.17 -8.39 -0.91 -8.11
None 0.76 0.81 0.92 0.63 1.38 1.11 -0.37 0.33 -1.26 0.58 1.04 0.28 1.05 -0.20 0.84
Nuclear Periphery 1.02 -5.06 -5.57 -9.82 3.98 0.53 -6.41 -7.51 -5.65 3.49 0.85 -6.37 -9.23 -9.29 4.65
Nuclear Periphery Foci 0.63 -3.93 -0.74 -5.33 3.85 1.80 -6.82 -5.47 -8.38 5.89 1.45 -5.53 -5.63 -9.82 5.07
Nucleolus -0.78 -2.87 1.58 2.43 3.75 0.24 0.66 3.07 3.75 3.85 -0.23 -2.41 2.70 4.09 4.29
Nucleus 2.72 -10.24 -4.12 -22.27 9.37 5.81 -7.55 -19.98 -25.44 3.03 6.85 -14.85 -22.64 -33.77 9.01
Peroxisomes 0.15 4.95 3.90 12.37 -6.38 -9.62 4.26 11.77 18.31 1.06 -10.65 10.07 10.29 21.41 -2.29
Vacuole -0.45 -7.54 -17.68 -31.93 -5.66 -5.59 -9.94 -31.65 -26.59 1.82 -4.63 -12.98 -43.52 -40.56 -2.23
Vacuole Periphery -2.12 2.45 2.28 8.17 -0.39 -2.07 11.53 5.11 6.92 -2.99 -4.10 7.17 5.89 10.54 -1.43
Standard name
Human Ortholog
Description Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss
Localization
Cell Percentages mitochondrion (54%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Aim18

Aim18


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aim18-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available