Standard name
Human Ortholog
Description Protein of unknown function; GFP-fusion protein localizes to the mitochondria; null mutant is viable and displays reduced frequency of mitochondrial genome loss
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0.14 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0.05 – 0 0 0 0 0 0 0 0.11 0.06 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.06 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.06 0 0.12 0.16 0.29 0.07 0.2 – 0 0.05 0 0 0.17 0.27 0.33 0.24 0.14 0.09 0.14 0.11 0.1 0.1 0.14 0.14
Golgi 0 0 0.06 0 0 0 0 – 0 0 0 0 0 0 0 0.22 0.26 0.21 0.05 0.07 0 0.06 0.09 0.09
Mitochondria 0.92 0.9 0.59 0.71 0.55 0.88 0.69 – 0.93 0.83 0.94 0.91 0.76 0.54 0.48 0.07 0.09 0.05 0.59 0.66 0.66 0.46 0.34 0.36
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.05 0 0 0 0 0 0 – 0.05 0.05 0 0 0 0 0 0.05 0 0.11 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.19 0.2 0.16 0 0.09 – 0 0.06 0 0 0.24 0.48 0.37 0.28 0.27 0.3 0.16 0.08 0.16 0.25 0.3 0.27
SpindlePole 0 0 0.2 0.12 0.11 0.05 0.11 – 0.05 0.07 0 0.05 0.07 0.1 0.08 0.14 0.18 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.06 0.06 0 0.14 0.05 0.09 – 0.05 0.05 0 0 0.05 0 0 0.05 0.05 0.11 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 3 4 5 0 0 0 – 0 1 0 0 4 2 0 2 2 1 1 5 1 7 13 11
Bud 2 4 7 6 1 12 11 – 11 18 11 12 10 10 5 3 2 1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 1 0 – 0 0 1 0 0 0 0 0 0 0 0 1 0 0 1 1
Bud Site 1 0 0 1 0 3 1 – 8 4 10 4 0 0 0 3 0 0 – – – – – –
Cell Periphery 1 4 9 5 2 1 6 – 3 8 7 5 3 3 2 7 2 8 0 0 0 1 0 0
Cytoplasm 2 6 4 10 13 9 23 – 5 12 6 4 3 10 11 15 5 6 0 0 1 0 0 3
Endoplasmic Reticulum 1 15 2 1 0 2 1 – 2 1 0 2 1 0 1 1 3 2 0 0 0 1 1 1
Endosome 8 3 23 37 87 19 84 – 13 24 6 4 30 66 86 31 11 5 23 29 23 17 34 33
Golgi 5 1 11 2 2 0 0 – 1 0 0 0 6 6 10 29 20 12 7 19 8 9 21 20
Mitochondria 124 228 112 163 165 239 291 – 391 375 333 378 137 132 126 9 7 3 95 182 162 77 82 85
Nucleus 0 0 2 0 5 7 17 – 6 8 4 4 0 0 1 2 3 2 0 0 0 0 0 0
Nuclear Periphery 7 1 0 0 3 3 11 – 20 22 12 16 1 0 4 6 2 6 2 2 2 1 1 1
Nucleolus 2 1 0 2 2 3 5 – 12 16 11 15 2 3 3 5 1 0 0 0 0 2 3 0
Peroxisomes 6 8 35 45 48 8 40 – 13 25 7 11 44 118 96 37 21 17 26 20 40 42 73 64
SpindlePole 3 5 38 27 33 13 47 – 20 31 13 20 12 25 20 18 14 1 2 2 2 1 3 3
Vac/Vac Membrane 6 16 11 7 42 13 40 – 22 22 10 13 9 5 11 6 4 6 0 7 0 1 1 2
Unique Cell Count 135 252 189 230 301 271 424 421 452 356 414 180 246 262 131 78 56 163 275 248 168 241 234
Labelled Cell Count 168 295 258 311 403 333 577 527 567 431 488 262 380 376 174 97 70 163 275 248 168 241 234


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 4.9 6.3 5.7 6.8 4.7 6.0 – 5.3 5.5 5.3 5.4 6.0 6.4 6.4 7.7 7.9 8.6 5.5 5.9 5.9
Std Deviation (1e-4) 1.0 0.7 1.7 1.1 2.0 1.4 1.5 – 1.4 1.4 1.2 1.1 1.1 1.7 1.6 1.7 1.8 2.2 1.6 1.8 2.0
Intensity Change (Log2) – – – -0.14 0.11 -0.43 -0.08 – -0.24 -0.18 -0.25 -0.22 -0.07 0.04 0.02 0.29 0.33 0.45 -0.18 -0.09 -0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 – –
Bud Neck – – – – – – – – – – – – 0 – –
Bud Site – – – – – – – – – – – – 0 – –
Cell Periphery – – – – – – – – – – – – 0 – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 – –
Endosome – – – – – – – – – – – – 0 – –
Golgi – – – – – – – – – – – – 0 – –
Mitochondria – – – – – – – – – – – – 0 – –
Nuclear Periphery – – – – – – – – – – – – 0 – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 – –
Peroxisomes – – – – – – – – – – – – 0 – –
SpindlePole – – – – – – – – – – – – 0 – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 – –
Cytoplasm – – – – – – – – – – – – 0 – –
Nucleus – – – – – – – – – – – – 0 – –
Vacuole – – – – – – – – – – – – -0.5 – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.0074 0.772 0.6874 0.6768 0.4751 0.9017 2.2939 3.3857 3.1285 3.4054 3.1179 2.6809 4.9197 4.8912 4.7352 4.5272 4.5712 4.961
Actin 0.0511 0.0102 0.0462 0.0183 0.0289 0.0191 0.0461 0.0215 0.0302 0.0098 0.0274 0.0241 0.0121 0.0894 0.1639 0.0008 0.0085 0.0875
Bud 0.0008 0.0005 0.001 0.0002 0.0005 0.0004 0.0005 0.0005 0.0009 0.0019 0.0005 0.0002 0.0014 0.0011 0.0014 0.0001 0.0007 0.0003
Bud Neck 0.0009 0.0006 0.002 0.0024 0.0002 0.0015 0.0004 0.0006 0.0006 0.0002 0.0004 0.0004 0.0007 0.0004 0.0009 0.0001 0.0002 0.0016
Bud Periphery 0.0014 0.001 0.0013 0.0004 0.0013 0.0008 0.0009 0.001 0.0024 0.0041 0.002 0.0005 0.0013 0.0013 0.0029 0.0002 0.0018 0.0008
Bud Site 0.0027 0.0092 0.0197 0.0004 0.0008 0.0009 0.0013 0.0042 0.0032 0.0007 0.0008 0.0004 0.0027 0.0042 0.0033 0.0002 0.0017 0.0007
Cell Periphery 0.0004 0.0014 0.0003 0.0001 0.0002 0.0002 0.0005 0.0072 0.0017 0.0001 0.0009 0.0003 0.0001 0.0016 0.0016 0.0001 0.0002 0.0008
Cytoplasm 0.0041 0.0101 0.0014 0.0003 0.0003 0.0004 0.0014 0.0145 0.0031 0.0012 0.001 0.0007 0.0237 0.0055 0.0025 0.0001 0.0002 0.0019
Cytoplasmic Foci 0.0373 0.038 0.0413 0.0251 0.0139 0.0262 0.0285 0.0217 0.0201 0.0134 0.0263 0.0244 0.0475 0.0369 0.027 0.0155 0.0072 0.0249
Eisosomes 0.0006 0.0008 0.0008 0.0002 0.0006 0.0003 0.0012 0.0027 0.0008 0.0001 0.0004 0.0006 0.0001 0.0025 0.0018 0.0001 0.0003 0.0019
Endoplasmic Reticulum 0.0017 0.0028 0.0006 0.0001 0.0006 0.0002 0.0028 0.0007 0.0006 0.0004 0.001 0.0009 0.0014 0.0019 0.0011 0.0001 0.0002 0.0005
Endosome 0.0709 0.0667 0.047 0.0302 0.0309 0.0399 0.0888 0.0543 0.0605 0.0859 0.0912 0.0373 0.0843 0.0506 0.0358 0.0397 0.0437 0.0326
Golgi 0.1047 0.1293 0.0444 0.0251 0.0335 0.082 0.1138 0.1035 0.1003 0.0848 0.0956 0.1064 0.27 0.0657 0.0691 0.0675 0.0702 0.087
Lipid Particles 0.0873 0.0449 0.0576 0.0253 0.1289 0.0555 0.0412 0.0264 0.0168 0.0258 0.013 0.017 0.0025 0.0018 0.0474 0.0042 0.0073 0.0188
Mitochondria 0.4329 0.5434 0.5856 0.5646 0.6397 0.6256 0.5813 0.6303 0.6878 0.7354 0.6456 0.6902 0.0682 0.6651 0.5167 0.8375 0.8045 0.6632
None 0.0025 0.0008 0.0077 0.0002 0.0012 0.0007 0.0137 0.0067 0.0012 0.0002 0.0002 0.0005 0.0084 0.0011 0.0013 0.0001 0.0002 0.0038
Nuclear Periphery 0.0093 0.0009 0.0016 0.0004 0.0028 0.0001 0.0008 0.0005 0.0002 0.0002 0.0002 0.0005 0.0397 0.0002 0.0003 0 0.0002 0.0001
Nucleolus 0.0018 0.0001 0.0015 0.0001 0.0003 0.0004 0.0005 0.0006 0.0002 0.0001 0.0001 0.0001 0.0054 0.0001 0.0002 0 0.0002 0.0001
Nucleus 0.0099 0.0002 0.0007 0.0001 0.0004 0.0002 0.0003 0.0003 0.0002 0.0001 0.0001 0.0001 0.3234 0.0002 0.0002 0 0.0002 0.0001
Peroxisomes 0.1684 0.1344 0.1346 0.3049 0.1108 0.1413 0.0639 0.0939 0.0598 0.0236 0.0828 0.0919 0.014 0.0678 0.1088 0.0313 0.0497 0.0716
Punctate Nuclear 0.0019 0.0002 0.0012 0.0005 0.0007 0.0003 0.0003 0.0002 0.0001 0.0001 0.0001 0.0002 0.0902 0.0001 0.0006 0 0.0001 0.0001
Vacuole 0.0048 0.0019 0.0018 0.0005 0.0013 0.0017 0.0073 0.0062 0.0063 0.0066 0.0046 0.0013 0.0015 0.0011 0.0076 0.0008 0.001 0.0007
Vacuole Periphery 0.0046 0.0026 0.0019 0.0006 0.002 0.0024 0.0045 0.0026 0.0029 0.0053 0.006 0.0022 0.0014 0.0013 0.0053 0.0016 0.002 0.001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.1654 21.4789 8.5821 6.0608 13.1798 12.9306 16.7056 9.5839 8.2398 12.9706
Translational Efficiency 1.2669 1.068 0.6235 0.6678 0.9177 0.898 1.3486 1.4187 1.1659 1.1577

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
294 1549 1633 1667 1022 2623 377 1201 1316 4172 2010 2868

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 672.12 784.35 944.65 902.37 735.88 815.70 928.12 975.20 721.64 804.06 941.55 932.87
Standard Deviation 89.61 104.60 115.31 120.34 86.81 99.51 114.69 137.51 91.39 102.56 115.38 132.77
Intensity Change Log 2 — 0.222779 0.491061 0.425000 — 0.148568 0.334841 0.406228 — 0.184470 0.411526 0.415219

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.003215 0.013669 0.008939 0.016355 0.004548 0.005631 0.019909 0.081325 0.004250 0.008616 0.010996 0.043562
Bud Neck 0.008883 0.004031 0.002442 0.001828 0.005466 0.002247 0.004994 0.002578 0.006229 0.002909 0.002921 0.002142
Bud Site 0.008867 0.017305 0.017821 0.026886 0.003678 0.004806 0.042849 0.062213 0.004837 0.009447 0.022515 0.041680
Cell Periphery 0.000996 0.000614 0.000152 0.000430 0.000265 0.000186 0.000433 0.001161 0.000429 0.000345 0.000205 0.000736
Cytoplasm 0.008964 0.004825 0.005341 0.007637 0.004593 0.002172 0.005593 0.013563 0.005570 0.003157 0.005388 0.010118
Cytoplasmic Foci 0.039732 0.093108 0.175413 0.082039 0.101500 0.073263 0.118227 0.089107 0.087701 0.080632 0.164688 0.084999
Eisosomes 0.000128 0.000185 0.000100 0.000154 0.000157 0.000114 0.000165 0.000353 0.000150 0.000140 0.000112 0.000238
Endoplasmic Reticulum 0.000414 0.000281 0.001876 0.001136 0.000208 0.000111 0.002131 0.001799 0.000254 0.000175 0.001923 0.001414
Endosome 0.066512 0.160461 0.160036 0.126882 0.139438 0.136377 0.147082 0.085383 0.123146 0.145319 0.157606 0.109504
Golgi 0.070734 0.162483* 0.216839* 0.169383* 0.167326* 0.166142* 0.116117* 0.189410* 0.145747* 0.164784* 0.197947* 0.177769*
Lipid Particles 0.005653 0.010139 0.004890 0.001954 0.026791 0.005534 0.004471 0.001633 0.022069 0.007244 0.004811 0.001819
Mitochondria 0.681091* 0.342536* 0.170672* 0.447709* 0.363339* 0.482128* 0.270626* 0.311415* 0.434326* 0.430299* 0.189420* 0.390635*
Mitotic Spindle 0.004053 0.010307 0.117313* 0.025422 0.006483 0.009820 0.058463 0.024737 0.005940 0.010001 0.106274* 0.025135
None 0.001185 0.000262 0.000134 0.000143 0.000268 0.000160 0.000136 0.000531 0.000473 0.000198 0.000135 0.000306
Nuclear Periphery 0.000090 0.000040 0.000452 0.000184 0.000079 0.000039 0.000157 0.000096 0.000081 0.000040 0.000397 0.000147
Nuclear Periphery Foci 0.000259 0.000202 0.001285 0.000432 0.000262 0.000112 0.000937 0.001105 0.000261 0.000145 0.001220 0.000714
Nucleolus 0.000869 0.000533 0.000935 0.000149 0.000667 0.000139 0.000357 0.000028 0.000712 0.000285 0.000827 0.000098
Nucleus 0.006768 0.000449 0.001224 0.000420 0.000983 0.000160 0.000898 0.000565 0.002275 0.000268 0.001163 0.000481
Peroxisomes 0.084223 0.171902* 0.054968 0.057357 0.167379* 0.102339* 0.177351* 0.116314* 0.148802* 0.128167* 0.077922 0.082046
Vacuole 0.002756 0.003877 0.042564 0.020985 0.003320 0.004836 0.019059 0.010974 0.003194 0.004480 0.038155 0.016793
Vacuole Periphery 0.004607 0.002791 0.016604 0.012513 0.003250 0.003683 0.010044 0.005711 0.003553 0.003352 0.015374 0.009665

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.14 -3.16 -5.71 -1.20 -3.55 -0.95 -3.90 -14.17 -14.07 -9.36 -4.21 -4.89 -14.83 -13.35 -11.72
Bud Neck 1.74 2.28 2.54 4.00 2.33 2.85 0.37 2.49 -0.92 3.47 3.09 3.01 3.81 2.04 2.95
Bud Site -2.58 -3.22 -5.44 -3.65 -3.49 -1.30 -6.92 -14.66 -14.40 -2.87 -4.18 -10.53 -16.77 -14.70 -7.29
Cell Periphery 1.49 3.99 2.62 1.10 -4.80 2.19 -2.83 -6.03 -6.71 -4.78 1.08 4.03 -3.42 -4.28 -7.54
Cytoplasm 1.35 1.15 0.52 -2.97 -2.39 2.29 -0.68 -6.10 -11.62 -6.14 2.26 0.25 -3.26 -10.30 -6.31
Cytoplasmic Foci -10.42 -23.61 -8.22 2.80 19.80 6.98 -2.11 2.68 -4.17 3.91 2.20 -16.61 0.87 -1.65 20.00
Eisosomes -3.20 2.05 -1.69 2.01 -5.52 3.66 -0.51 -9.90 -14.17 -8.59 0.99 3.72 -6.90 -9.50 -12.79
Endoplasmic Reticulum 1.07 -5.17 -2.67 -3.45 2.26 2.40 -2.43 -4.27 -4.56 0.42 1.84 -6.39 -5.42 -5.89 1.72
Endosome -11.24 -11.65 -7.59 5.63 6.00 0.41 -0.69 8.12 10.60 6.63 -4.09 -5.75 2.47 9.39 10.75
Golgi -10.99 -16.43 -11.34 -0.96 6.36 0.12 5.52 -2.57 -3.16 -7.69 -3.39 -7.55 -5.05 -2.59 3.15
Lipid Particles -2.19 0.39 2.41 5.45 4.39 6.77 6.90 8.09 4.57 2.63 5.91 6.94 8.29 7.19 5.21
Mitochondria 15.01 23.73 10.35 -8.11 -25.09* -9.03 4.59 3.46 14.13 -2.14 0.25 20.26 3.49 4.59 -22.11*
Mitotic Spindle -3.60 -21.01* -8.80 -6.48 16.41 -2.65 -7.36 -7.66 -6.43 4.63 -3.94 -22.08 -11.37 -9.28 17.23
None 1.49 1.69 1.68 1.54 -0.43 1.87 2.49 -0.57 -0.90 -0.97 1.92 2.37 0.90 -0.59 -0.99
Nuclear Periphery 1.08 -4.45 -1.52 -3.46 3.46 2.20 -1.81 -0.43 -1.54 1.13 2.37 -5.47 -2.02 -3.81 4.06
Nuclear Periphery Foci 0.44 -4.31 -0.89 -1.60 3.60 3.69 -2.53 -1.21 -1.45 -0.07 2.62 -5.46 -1.48 -1.92 1.66
Nucleolus 0.52 -0.12 1.25 1.17 1.79 2.75 1.44 3.48 1.67 2.56 1.99 -0.23 3.14 1.38 2.06
Nucleus 2.00 1.74 2.00 0.00 2.20 2.37 0.11 0.87 -1.52 0.97 2.66 1.36 2.29 -1.23 2.30
Peroxisomes -7.40 2.72 2.49 15.92 -0.47 7.66 -0.67 5.32 -1.94 4.20 2.86 9.54 9.32 9.75 -0.77
Vacuole -1.39 -18.06 -10.74 -10.59 8.25 -2.32 -5.41 -5.92 -4.86 2.78 -2.49 -18.92 -12.18 -11.36 10.32
Vacuole Periphery 1.95 -9.36 -6.91 -13.10 3.51 -0.90 -4.09 -3.13 -2.87 2.48 0.46 -13.33 -9.68 -11.94 6.02
Standard name
Human Ortholog
Description Protein of unknown function; GFP-fusion protein localizes to the mitochondria; null mutant is viable and displays reduced frequency of mitochondrial genome loss
Localization
Cell Percentages mitochondrion (73%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Aim34

Aim34


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aim34-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available