Standard name
Human Ortholog
Description D-Arabinono-1,4-lactone oxidase; catalyzes the final step in biosynthesis of dehydro-D-arabinono-1,4-lactone, which is protective against oxidative stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.09 0.11 0 0 0 0 0.05 0.06
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0.05 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0.08 0.09 0.07 0.06 0 0 0 0 0 0 0 0 0.08 0.14 0.62 0.64 0.77 0.09 0.14 0.07 0.13 0.15 0.11
Mitochondria 0.94 0.91 0.94 0.95 0.88 0.92 0.84 0.9 0.9 0.93 0.88 0.93 0.93 0.94 0.84 0.05 0 0.05 0.8 0.65 0.76 0.71 0.66 0.61
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.07 0 0 0 0 0.07 0.08 0.06 0.06 0.09 0.12 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.12 0.05 0.06 0.14 0.11 0.12 0.23 0.17 0.17 0.16 0.19 0.18 0.08 0 0 0.09 0.11 0.08 0 0 0 0 0 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.08 0.11 0 0 0.13 0 0 0 0 0 0 0 0.08 0 0.11 0.09 0.18 0.1 0.05 0.14 0 0.06 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 5 3 2 0 2 0 1 0 0 0 0 0 3 9 24 9 7 3 4 6 1 7 6
Bud 0 2 2 1 1 1 2 4 3 2 2 2 0 0 1 4 0 0 0 1 2 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 1 0 0 1 0 3 1 1 0 1 0 0 0 0 1 2 – – – – – –
Cell Periphery 2 4 2 0 0 0 0 1 0 0 1 4 1 0 2 13 5 3 0 0 1 0 0 0
Cytoplasm 1 1 2 3 3 3 7 5 6 3 2 3 1 3 3 10 4 0 0 1 0 0 0 0
Endoplasmic Reticulum 0 1 1 0 0 0 0 1 1 2 0 0 0 2 4 2 0 0 0 1 0 0 0 0
Endosome 1 0 1 0 1 1 0 0 0 0 0 0 0 1 2 1 2 0 1 1 2 0 0 2
Golgi 1 15 11 5 8 1 0 6 0 1 0 1 2 13 28 114 65 47 14 28 9 7 22 13
Mitochondria 147 180 120 69 125 162 199 301 180 212 107 113 94 154 169 9 3 3 130 136 104 43 101 75
Nucleus 0 0 0 0 1 2 1 3 3 2 1 0 0 0 1 2 0 1 0 0 0 0 0 0
Nuclear Periphery 11 1 2 2 5 12 20 21 12 20 15 15 1 1 1 4 4 2 1 2 0 0 1 0
Nucleolus 19 9 7 10 15 22 54 57 35 36 23 22 8 4 7 16 11 5 1 2 0 0 1 6
Peroxisomes 1 0 0 0 0 1 0 1 0 0 0 0 0 1 2 4 0 0 0 1 5 0 7 7
SpindlePole 0 0 1 0 0 0 1 1 2 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Vac/Vac Membrane 13 22 5 2 19 7 6 14 4 4 3 4 8 4 22 16 18 6 7 28 0 3 5 4
Unique Cell Count 157 197 127 73 142 176 238 334 200 228 122 121 101 163 201 184 102 61 163 212 137 61 154 124
Labelled Cell Count 197 240 158 94 178 215 290 419 247 284 154 165 115 186 251 219 122 76 163 212 137 61 154 124


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 21.6 25.7 19.6 16.2 16.7 16.7 14.3 15.8 15.5 14.0 13.7 14.0 27.8 28.9 28.5 23.0 24.9 28.4 22.2 25.3 24.3
Std Deviation (1e-4) 6.3 7.5 6.1 5.8 5.3 5.5 5.1 5.2 5.6 4.6 5.4 4.9 7.5 8.2 8.7 6.1 7.4 6.4 6.5 6.9 9.1
Intensity Change (Log2) – – – -0.28 -0.23 -0.23 -0.46 -0.31 -0.33 -0.48 -0.51 -0.49 0.5 0.56 0.54 0.23 0.35 0.53 0.18 0.37 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 3.3 2.2 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 2.2 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi -0.5 -1.0 -3.6 0 0 0 0 -3.3 -2.9 -2.2 -0.2 1.4 9.4 8.8 9.5
Mitochondria 0 -1.9 -0.8 -3.0 -1.5 -1.4 -0.6 -1.9 -0.4 -0.4 0 -2.8 -15.8 -13.8 -12.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 2.1 2.6 2.1 1.9 2.7 3.4 3.4 0 0 0 0 0 0
Nucleolus 2.0 1.5 2.0 4.2 3.2 3.2 2.8 3.2 3.1 0.7 0 -0.9 1.1 1.5 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.7 0 0 0.1 0 0 0 0 1.3 0 2.2 1.6 3.4 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 18.2395 22.501 19.9309 18.4227 16.9237 20.1275 15.0205 20.2473 16.3495 14.0575 18.4179 17.4153 12.0222 21.9011 22.6188 21.116 20.3064 20.4078
Actin 0.0388 0.0059 0.0024 0.0072 0.0198 0.001 0.0828 0.0009 0.0094 0.0835 0 0.0211 0.0009 0.0011 0.003 0.0002 0.0001 0.0043
Bud 0.0007 0.0017 0.0009 0.0015 0.0006 0.0001 0.001 0 0.0011 0.0037 0 0.0002 0.0001 0.0002 0.0004 0.0009 0 0.0001
Bud Neck 0.0019 0.001 0.0002 0.0002 0.0001 0 0.0015 0.0001 0.0008 0.0003 0 0.0001 0.0001 0.0001 0.0001 0 0 0
Bud Periphery 0.0016 0.0045 0.0022 0.0052 0.0008 0.0002 0.0016 0.0002 0.0015 0.0062 0 0.0004 0.0002 0.0005 0.0007 0.0022 0 0.0001
Bud Site 0.0042 0.0056 0.0014 0.0009 0.0018 0 0.0074 0.0001 0.005 0.015 0 0.0002 0.0004 0.001 0.0022 0.0002 0 0.0001
Cell Periphery 0.0006 0.003 0.0012 0.001 0.0003 0.0002 0.0007 0.0007 0.0016 0.0014 0 0.0003 0.0003 0.0012 0.0005 0.0007 0 0.0001
Cytoplasm 0.0009 0.0075 0.0002 0 0 0 0.0003 0 0.0012 0.0001 0 0 0 0 0 0 0 0.0001
Cytoplasmic Foci 0.0054 0.002 0.0045 0.0019 0.0006 0.0008 0.0045 0.0001 0.0092 0.0036 0 0.0008 0.0056 0.0005 0.0023 0.0002 0.0001 0.0013
Eisosomes 0.0015 0.0003 0.0002 0.0004 0.0002 0.0001 0.0007 0.0002 0.0005 0.0006 0 0.0001 0.0002 0.0002 0.0004 0.0001 0.0001 0.0002
Endoplasmic Reticulum 0.0014 0.0031 0.0002 0.0001 0 0 0.0004 0 0.0004 0.0002 0 0 0 0.0001 0 0 0 0.0002
Endosome 0.0074 0.0102 0.0103 0.0007 0.0003 0.0007 0.0229 0.0004 0.0184 0.0151 0 0.0026 0.0037 0.0019 0.0027 0.0009 0.0001 0.0039
Golgi 0.0064 0.0083 0.0117 0.0018 0.0006 0.0016 0.01 0.0006 0.0129 0.007 0.0001 0.0033 0.0035 0.0017 0.002 0.0002 0.0004 0.0031
Lipid Particles 0.0366 0.008 0.0084 0.0236 0.0041 0.0085 0.0139 0.005 0.013 0.0352 0.0036 0.0105 0.0475 0.0111 0.019 0.002 0.0023 0.0047
Mitochondria 0.7581 0.807 0.9332 0.933 0.9519 0.9751 0.7924 0.9783 0.8837 0.7623 0.9899 0.9479 0.9063 0.9105 0.9481 0.9352 0.9925 0.9786
None 0.0151 0.0004 0.0002 0.0001 0 0 0.0003 0 0.0002 0.0006 0 0 0.0001 0 0.0001 0 0 0.0002
Nuclear Periphery 0.0483 0.0015 0.0002 0.0001 0 0.0001 0.0003 0 0.0001 0.0006 0 0 0.0001 0.0003 0.0001 0 0 0.0002
Nucleolus 0.01 0.0151 0.003 0.0022 0.0004 0.0001 0.0022 0.0003 0.0088 0.0038 0.0001 0.0004 0.0013 0.0006 0.001 0.003 0.0001 0
Nucleus 0.0071 0.0028 0.0007 0.0006 0.0002 0.0001 0.0005 0.0001 0.0007 0.0012 0 0.0002 0.0002 0.0003 0.0003 0.0003 0 0
Peroxisomes 0.01 0.0031 0.0031 0.0086 0.0086 0.0032 0.0249 0.0017 0.0075 0.0065 0.0005 0.0017 0.02 0.0052 0.0045 0.0023 0.0016 0.0016
Punctate Nuclear 0.0037 0.0002 0.0002 0.0001 0 0 0.0001 0 0.0001 0.0002 0 0 0.0001 0 0.0003 0 0 0.0003
Vacuole 0.0089 0.045 0.0063 0.0021 0.002 0.002 0.0148 0.0029 0.01 0.0233 0.0006 0.0029 0.0028 0.0155 0.0015 0.0205 0.0002 0.0002
Vacuole Periphery 0.0314 0.064 0.0092 0.0087 0.0076 0.0063 0.0168 0.0083 0.014 0.0298 0.005 0.0072 0.0066 0.048 0.0108 0.031 0.0025 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 63.1614 66.1327 69.9064 73.2041 71.0329 74.6356 64.8166 56.8389 64.5617 70.0165
Translational Efficiency 1.8371 1.4923 1.5096 1.7191 1.3172 1.4942 1.456 1.6796 1.6692 1.4144

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1816 1250 324 476 110 1691 160 1023 1926 2941 484 1499

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1608.40 1400.76 2816.89 2576.08 1913.88 1611.60 2503.25 2474.61 1625.85 1521.99 2713.21 2506.83
Standard Deviation 461.31 471.32 792.33 781.31 462.17 480.41 703.01 815.19 466.78 487.83 778.08 805.97
Intensity Change Log 2 — -0.199416 0.808477 0.679551 — -0.248007 0.387302 0.370701 — -0.225615 0.594955 0.519971

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.003570 0.009692 0.016429 0.007886 0.003699 0.004105 0.020133 0.005786 0.003577 0.006479 0.017653 0.006453
Bud Neck 0.004227 0.006862 0.010919 0.007082 0.013542 0.002161 0.021028 0.005373 0.004759 0.004159 0.014261 0.005916
Bud Site 0.018037 0.024254 0.007431 0.027169 0.006510 0.009558 0.023677 0.036489 0.017379 0.015804 0.012801 0.033529
Cell Periphery 0.001740 0.004044 0.000499 0.002067 0.000694 0.002396 0.001823 0.004963 0.001680 0.003096 0.000937 0.004044
Cytoplasm 0.010095 0.006734 0.002075 0.005088 0.002864 0.003247 0.007495 0.009558 0.009682 0.004729 0.003866 0.008138
Cytoplasmic Foci 0.016334 0.017166 0.054903 0.020867 0.078827 0.009009 0.081322 0.013433 0.019903 0.012476 0.063637 0.015793
Eisosomes 0.000542 0.002755 0.001534 0.000731 0.001528 0.000819 0.001755 0.000821 0.000599 0.001642 0.001607 0.000792
Endoplasmic Reticulum 0.000490 0.000652 0.000341 0.000704 0.000636 0.000341 0.000740 0.000603 0.000498 0.000473 0.000473 0.000635
Endosome 0.006332 0.007963 0.004319 0.007222 0.015640 0.004312 0.006996 0.006295 0.006864 0.005864 0.005204 0.006589
Golgi 0.015999 0.014861 0.023013 0.045325 0.025731 0.016238 0.023595 0.040498 0.016555 0.015653 0.023206 0.042031
Lipid Particles 0.005587 0.005357 0.006328 0.004066 0.012088 0.002245 0.003188 0.001514 0.005958 0.003567 0.005290 0.002324
Mitochondria 0.835579* 0.843844* 0.716356* 0.703892* 0.688279* 0.904929* 0.625676* 0.744686* 0.827166* 0.878966* 0.686379* 0.731732*
Mitotic Spindle 0.001922 0.001602 0.011568 0.008427 0.001324 0.001237 0.015656 0.005304 0.001887 0.001392 0.012919 0.006296
None 0.004944 0.004424 0.006245 0.004419 0.002806 0.003797 0.003261 0.003697 0.004822 0.004064 0.005259 0.003926
Nuclear Periphery 0.000652 0.000158 0.004652 0.003131 0.000816 0.000244 0.003298 0.000566 0.000661 0.000207 0.004204 0.001381
Nuclear Periphery Foci 0.000626 0.000395 0.001631 0.000961 0.002354 0.000316 0.001469 0.000315 0.000725 0.000349 0.001577 0.000520
Nucleolus 0.002248 0.003398 0.004471 0.002294 0.011896 0.000781 0.009348 0.001153 0.002799 0.001894 0.006083 0.001515
Nucleus 0.001641 0.001106 0.000829 0.001893 0.001552 0.000503 0.003013 0.000898 0.001636 0.000759 0.001551 0.001214
Peroxisomes 0.004867 0.013165 0.011422 0.004275 0.021129 0.005413 0.026005 0.002520 0.005796 0.008708 0.016243 0.003077
Vacuole 0.029937 0.018560 0.035827 0.040169 0.061522 0.013484 0.053261 0.033050 0.031741 0.015642 0.041590 0.035311
Vacuole Periphery 0.034631 0.013009 0.079209 0.102332* 0.046563 0.014866 0.067261 0.082478 0.035313 0.014076 0.075259 0.088783

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.40 -3.38 -3.13 1.03 2.16 -0.25 -2.68 -1.37 -1.44 2.36 -3.30 -4.33 -3.03 -0.00 3.40
Bud Neck -2.68 -4.96 -2.59 -0.36 2.19 3.37 -1.81 2.30 -4.68 5.11 0.90 -6.79 -1.80 -2.90 5.73
Bud Site -1.79 4.28 -1.96 -0.76 -4.03 -1.24 -3.88 -7.06 -6.66 -2.14 0.65 1.54 -4.76 -5.48 -5.95
Cell Periphery -2.55 6.50 -0.57 1.90 -2.87 -2.77 -0.91 -5.21 -2.57 -2.16 -2.63 1.71 -3.91 -1.24 -4.42
Cytoplasm 1.46 3.41 1.50 0.22 -1.61 -0.08 -1.67 -2.52 -3.83 -0.10 2.80 1.97 -0.02 -3.00 -2.06
Cytoplasmic Foci -0.40 -5.06 -1.56 -1.31 4.03 5.15 -0.25 4.76 -2.27 5.70 4.15 -6.71 1.54 -2.08 7.22
Eisosomes -7.59 -4.85 -2.06 6.71 3.67 2.10 -0.36 2.03 0.01 2.11 -7.78 -5.11 -2.12 5.56 3.86
Endoplasmic Reticulum -1.89 1.53 -1.62 -0.38 -2.39 1.50 -0.48 0.18 -3.33 0.74 0.42 0.00 -1.82 -2.24 -1.19
Endosome -1.37 1.68 -0.75 0.45 -1.92 2.02 1.49 1.65 -2.12 0.39 1.22 1.53 0.26 -0.90 -1.27
Golgi 0.51 -2.04 -5.52 -5.68 -3.72 1.71 0.38 -2.34 -6.75 -3.08 0.48 -2.21 -8.33 -8.92 -5.06
Lipid Particles 0.21 -0.66 1.46 1.18 1.97 1.60 1.43 1.72 1.74 2.58 2.77 0.63 4.39 2.55 4.27
Mitochondria -0.78 6.76 8.24 8.43 0.72 -6.39 1.77 -1.46 14.67 -4.34 -6.61 9.19 9.51 15.99 -2.71
Mitotic Spindle 0.35 -2.74 -2.84 -2.95 0.59 0.06 -3.72 -3.72 -3.58 2.61 0.65 -4.26 -4.05 -4.62 2.28
None 0.92 -1.43 1.14 0.11 2.31 -1.47 -1.21 -2.42 0.18 -0.85 1.36 -0.66 2.10 0.41 2.55
Nuclear Periphery 4.54 -3.17 -3.14 -3.81 1.04 2.21 -1.99 0.93 -3.52 2.22 4.11 -3.77 -2.65 -4.61 2.92
Nuclear Periphery Foci 2.80 -3.58 -1.84 -3.14 2.06 3.88 1.43 3.96 0.10 3.64 3.97 -3.88 2.13 -1.92 4.80
Nucleolus -1.25 -2.59 -0.08 1.22 2.58 2.45 0.42 2.37 -1.78 2.51 1.38 -2.56 2.42 0.98 3.79
Nucleus 1.21 1.44 -0.75 -1.24 -1.42 1.73 -1.65 0.13 -2.04 1.72 2.54 -0.80 -0.19 -2.15 0.63
Peroxisomes -4.84 -2.41 0.57 4.85 2.55 2.62 -0.70 3.16 3.11 4.46 -2.86 -4.08 3.87 6.04 5.20
Vacuole 4.39 -1.55 -2.60 -5.29 -0.85 3.99 0.48 2.27 -7.23 2.55 7.49 -2.63 -1.55 -8.70 1.53
Vacuole Periphery 8.59 -5.70 -8.05 -10.90 -2.05 3.31 -1.38 -3.33 -13.23 -1.43 9.13 -6.15 -11.24 -17.21 -1.83
Standard name
Human Ortholog
Description D-Arabinono-1,4-lactone oxidase; catalyzes the final step in biosynthesis of dehydro-D-arabinono-1,4-lactone, which is protective against oxidative stress
Localization
Cell Percentages mitochondrion (82%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Alo1

Alo1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Alo1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available