Standard name
Human Ortholog
Description Cytoplasmic aspartyl aminopeptidase with possible vacuole function; Cvt pathway cargo protein; cleaves unblocked N-terminal acidic amino acids from peptide substrates; forms a 12-subunit homo-oligomer; M18 metalloprotease family
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.88 0.96 0.84 0.98 0.84 0.88 0.84 0.73 0.71 0.69 0.67 0.62 0.95 0.99 0.99 0.92 0.92 0.97 0.75 0.76 0.6 0.89 0.88 0.81
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.07 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0.05 0.11 0.07 0.06 0.1 0.08 0.23 0.23 0.26 0.32 0.41 0 0 0 0 0 0 0.08 0.06 0.15 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.05 0 0.06 0 0.06 0.1 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 3 1 0 0 0 0 0 1 0 0 4 2 2 0 2 2
Bud 5 0 3 1 0 0 0 1 2 2 1 2 0 0 0 0 0 0 8 3 1 0 5 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 1 4 3 3 5 4 6 1 4 0 1 0 6 3 3 0 0 0 0 0 0
Cytoplasm 257 73 74 95 107 110 134 156 94 121 64 78 143 195 195 180 197 141 228 73 68 92 198 167
Endoplasmic Reticulum 2 1 1 0 3 5 6 1 4 7 6 4 2 0 0 4 7 5 1 0 2 0 2 3
Endosome 5 0 6 0 5 0 1 3 0 1 0 0 0 0 0 9 4 2 13 1 7 1 3 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 3 0 1 0 0 0 1 2
Mitochondria 35 4 10 7 8 13 13 48 31 45 31 51 3 1 1 1 0 0 25 5 17 2 3 5
Nucleus 1 0 0 2 1 1 2 2 1 2 0 1 2 1 0 1 3 1 0 0 0 1 0 0
Nuclear Periphery 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 1 3 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 2 0 9 1 4 1 2 4
Vac/Vac Membrane 6 0 4 0 8 3 10 21 2 7 1 5 3 2 1 6 17 4 3 1 1 1 3 5
Unique Cell Count 291 76 88 97 128 125 159 213 133 176 96 125 151 197 197 195 214 145 304 96 115 104 227 207
Labelled Cell Count 313 78 102 106 136 135 170 238 145 193 105 145 153 201 197 208 236 156 304 96 115 104 227 207


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 4.8 5.3 5.7 7.8 7.3 7.5 7.6 7.8 7.5 7.2 7.2 6.7 6.6 6.9 13.8 15.5 17.0 4.9 6.5 7.7
Std Deviation (1e-4) 1.3 1.4 1.7 1.7 2.2 1.6 1.7 1.8 1.7 1.7 2.0 1.8 2.0 1.9 1.8 3.0 3.4 4.3 1.5 2.9 2.3
Intensity Change (Log2) – – – 0.1 0.56 0.45 0.48 0.5 0.54 0.48 0.44 0.43 0.32 0.3 0.37 1.37 1.54 1.67 -0.13 0.29 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 –
Bud Neck – – – – – – – – – – – – – 0 –
Bud Site – – – – – – – – – – – – – 0 –
Cell Periphery – – – – – – – – – – – – – 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 –
Endosome – – – – – – – – – – – – – 0 –
Golgi – – – – – – – – – – – – – 0 –
Mitochondria – – – – – – – – – – – – – 0 –
Nuclear Periphery – – – – – – – – – – – – – 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 –
Peroxisomes – – – – – – – – – – – – – 0 –
SpindlePole – – – – – – – – – – – – – 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 –
Cytoplasm – – – – – – – – – – – – – 0 –
Nucleus – – – – – – – – – – – – – 0 –
Vacuole – – – – – – – – – – – – – 1.1 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.2342 1.4817 1.213 0.8463 0.7915 1.411 2.0512 2.2214 2.3353 1.8662 3.3014 2.1522 2.7467 2.3157 2.5623 2.8592 2.5342 3.1478
Actin 0.0147 0.0012 0.015 0.0204 0.0012 0.0055 0.0241 0.0172 0.0253 0.0455 0.0014 0.0144 0.04 0.0019 0.021 0.0074 0.0522 0.0101
Bud 0.0007 0.0016 0.0014 0.0011 0.0008 0.0018 0.0006 0.0048 0.0015 0.0005 0.0021 0.0008 0.0025 0.001 0.0024 0.004 0.0006 0.002
Bud Neck 0.0031 0.0006 0.0019 0.0056 0.0009 0.0043 0.0009 0.0011 0.0015 0.0002 0.0004 0.0056 0.0049 0.0003 0.0079 0.0006 0.0026 0.0048
Bud Periphery 0.0013 0.002 0.0023 0.0012 0.0006 0.0061 0.0005 0.007 0.0036 0.0003 0.0065 0.0023 0.0058 0.0009 0.0026 0.0069 0.0009 0.0052
Bud Site 0.0058 0.0055 0.0048 0.0023 0.0003 0.0015 0.0021 0.0204 0.0087 0.0007 0.0007 0.0004 0.0035 0.0053 0.0026 0.0014 0.0017 0.0004
Cell Periphery 0.0007 0.0004 0.0003 0.0002 0.0001 0.0002 0.0003 0.0011 0.001 0.0001 0.0003 0.0002 0.0009 0.0003 0.0004 0.0003 0.0003 0.0001
Cytoplasm 0.3617 0.5157 0.4097 0.4923 0.5785 0.4903 0.3299 0.3683 0.3298 0.309 0.3646 0.3819 0.3559 0.491 0.4967 0.4395 0.5017 0.4197
Cytoplasmic Foci 0.0665 0.0459 0.0595 0.0451 0.0881 0.0653 0.0713 0.0566 0.0669 0.063 0.0325 0.0706 0.0377 0.0389 0.0504 0.0648 0.0438 0.0568
Eisosomes 0.0005 0.0001 0.0003 0.0001 0.0001 0.0001 0.0005 0.0008 0.0007 0.0001 0.0002 0.0002 0.0011 0.0001 0.0003 0.0002 0.0005 0.0002
Endoplasmic Reticulum 0.011 0.0041 0.0063 0.0079 0.0042 0.0039 0.0036 0.0038 0.0042 0.0025 0.0029 0.0037 0.0083 0.0035 0.0045 0.0039 0.0096 0.0029
Endosome 0.0368 0.0269 0.0264 0.0369 0.053 0.0276 0.0337 0.0228 0.0297 0.0111 0.0629 0.0231 0.0212 0.0302 0.0259 0.0436 0.0223 0.0143
Golgi 0.0043 0.0019 0.005 0.002 0.0038 0.0029 0.017 0.0073 0.0095 0.005 0.0076 0.002 0.0063 0.0058 0.0059 0.0172 0.01 0.0021
Lipid Particles 0.0072 0.0008 0.0056 0.001 0.0004 0.0035 0.0086 0.0021 0.0101 0.0013 0.002 0.0041 0.0094 0.0012 0.0039 0.0175 0.019 0.001
Mitochondria 0.0054 0.0033 0.007 0.0021 0.0083 0.0047 0.0164 0.0033 0.0043 0.0014 0.0935 0.0009 0.0059 0.0025 0.0075 0.0359 0.0074 0.0018
None 0.4276 0.3684 0.4237 0.3551 0.2454 0.3546 0.4621 0.4681 0.4788 0.5345 0.357 0.4749 0.4555 0.4089 0.3475 0.2904 0.3014 0.4683
Nuclear Periphery 0.0139 0.0018 0.0032 0.0039 0.0015 0.006 0.001 0.0008 0.0032 0.0011 0.0081 0.0006 0.0128 0.0008 0.0021 0.0012 0.0025 0.001
Nucleolus 0.0018 0.0008 0.0008 0.0003 0.0001 0.0006 0.0003 0.0003 0.0005 0.0001 0.0003 0.0001 0.0006 0.0002 0.0008 0.0002 0.0008 0.0001
Nucleus 0.0115 0.005 0.0059 0.0076 0.003 0.0072 0.0017 0.0015 0.0015 0.0011 0.0016 0.0008 0.0099 0.0016 0.002 0.0013 0.0017 0.002
Peroxisomes 0.0079 0.0014 0.0086 0.0039 0.0044 0.0056 0.0146 0.0096 0.0115 0.019 0.0045 0.01 0.0071 0.0016 0.0082 0.0593 0.0112 0.0043
Punctate Nuclear 0.0082 0.0051 0.0052 0.0041 0.0011 0.0043 0.0068 0.001 0.0045 0.002 0.0015 0.002 0.0059 0.0008 0.0044 0.0009 0.0067 0.0017
Vacuole 0.0073 0.0064 0.0058 0.0053 0.003 0.0033 0.0032 0.0018 0.0024 0.0011 0.0246 0.0012 0.0035 0.0027 0.0023 0.0025 0.0023 0.0011
Vacuole Periphery 0.0022 0.001 0.0012 0.0014 0.0011 0.0008 0.0007 0.0002 0.0008 0.0003 0.0247 0.0003 0.0014 0.0007 0.0006 0.0011 0.0009 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 41.1998 18.0802 30.3109 36.375 39.3345 50.161 24.036 26.7331 37.8083 37.1171
Translational Efficiency 1.2434 1.5749 1.1048 1.2954 1.246 0.985 1.1179 1.2542 1.105 1.0827

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
316 391 87 1149 1900 2133 277 2164 2216 2524 364 3313

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 863.21 835.44 1147.01 888.23 851.60 748.11 1250.62 924.91 853.26 761.64 1225.86 912.19
Standard Deviation 142.28 166.67 214.82 181.52 136.37 103.52 224.68 177.04 137.29 119.82 226.71 179.46
Intensity Change Log 2 — -0.047175 0.410094 0.041222 — -0.186926 0.554396 0.119137 — -0.114886 0.483560 0.080442

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000258 0.000503 0.001130 0.000895 0.000115 0.000397 0.000748 0.000853 0.000135 0.000413 0.000839 0.000867
Bud Neck 0.004917 0.004777 0.003479 0.005943 0.005239 0.037236 0.003367 0.011047 0.005193 0.032207 0.003394 0.009277
Bud Site 0.009499 0.010980 0.015200 0.023659 0.003951 0.016585 0.012788 0.037216 0.004742 0.015717 0.013364 0.032514
Cell Periphery 0.000150 0.000136 0.000285 0.000158 0.000111 0.000129 0.000174 0.000136 0.000117 0.000130 0.000201 0.000144
Cytoplasm 0.656737* 0.666503* 0.381390* 0.526224* 0.675971* 0.440998* 0.476739* 0.587638* 0.673228* 0.475932* 0.453949* 0.566339*
Cytoplasmic Foci 0.176778 0.080481 0.081557 0.023463 0.132832 0.208215* 0.058322 0.022828 0.139099 0.188428* 0.063875 0.023048
Eisosomes 0.000060 0.000037 0.000095 0.000040 0.000044 0.000119 0.000050 0.000033 0.000046 0.000106 0.000060 0.000036
Endoplasmic Reticulum 0.000940 0.001419 0.006335 0.002198 0.002216 0.000707 0.004041 0.000884 0.002034 0.000818 0.004589 0.001340
Endosome 0.014898 0.008101 0.029078 0.005306 0.010502 0.024467 0.015981 0.009037 0.011129 0.021931 0.019112 0.007743
Golgi 0.001644 0.002559 0.009179 0.001509 0.001391 0.005358 0.000424 0.005702 0.001427 0.004925 0.002516 0.004248
Lipid Particles 0.002986 0.001976 0.006571 0.001415 0.003297 0.006775 0.003601 0.000890 0.003253 0.006032 0.004311 0.001072
Mitochondria 0.003200 0.000665 0.000615 0.001357 0.001099 0.011586 0.000196 0.003377 0.001399 0.009894 0.000296 0.002676
Mitotic Spindle 0.000395 0.007626 0.004754 0.028265 0.001179 0.011766 0.007810 0.047882 0.001067 0.011125 0.007080 0.041079
None 0.002422 0.011915 0.001331 0.007863 0.007241 0.003642 0.002125 0.006115 0.006554 0.004923 0.001936 0.006721
Nuclear Periphery 0.000171 0.000317 0.001698 0.001243 0.000294 0.000537 0.001167 0.001062 0.000276 0.000503 0.001294 0.001125
Nuclear Periphery Foci 0.003122 0.004801 0.020608 0.002002 0.001362 0.000337 0.014895 0.000661 0.001613 0.001029 0.016260 0.001126
Nucleolus 0.000421 0.002240 0.000535 0.000373 0.000813 0.002143 0.000250 0.000518 0.000757 0.002158 0.000318 0.000468
Nucleus 0.070542 0.144558* 0.260849* 0.236359* 0.100538 0.138282* 0.152290* 0.156831* 0.096260 0.139254* 0.178236* 0.184412*
Peroxisomes 0.002559 0.003131 0.007842 0.000960 0.001649 0.015970 0.004550 0.001644 0.001778 0.013981 0.005337 0.001407
Vacuole 0.047720 0.046632 0.166791 0.126451 0.049165 0.072402 0.240132* 0.103812 0.048959 0.068410 0.222603* 0.111664
Vacuole Periphery 0.000583 0.000643 0.000678 0.004316 0.000992 0.002346 0.000351 0.001833 0.000934 0.002082 0.000429 0.002694

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.06 -4.89 -3.06 -1.12 1.93 -3.37 -2.60 -2.73 -0.64 1.24 -3.85 -3.55 -4.29 -1.35 1.53
Bud Neck 0.01 0.20 -0.78 -0.70 -0.93 -16.10 -0.48 -5.09 13.56 -2.35 -15.73 -0.34 -4.63 13.86 -2.24
Bud Site -0.65 -1.07 -2.62 -1.74 -0.27 -10.08 -5.19 -9.66 -1.98 -2.70 -9.68 -5.10 -10.95 -2.04 -2.30
Cell Periphery 0.70 -5.70 1.01 0.11 6.46 -1.85 -4.94 1.96 3.06 5.75 -1.59 -7.47 0.90 2.16 7.78
Cytoplasm 0.35 10.18 14.55 14.62 -2.73 27.60 16.61 21.41 -6.17 -6.01 25.04 19.68 28.40 0.85 -5.99
Cytoplasmic Foci 7.88 7.68 15.04 9.18 3.28 -12.34 10.81 28.17 39.61 4.44 -8.81 12.84 31.90 39.86 5.46
Eisosomes 2.86 -3.92 1.22 -2.78 4.77 -10.07 -2.77 3.10 11.16 4.54 -9.44 -4.97 1.72 10.23 5.93
Endoplasmic Reticulum -2.96 -7.19 -10.48 -6.91 4.93 3.22 -2.88 2.06 -3.40 6.29 2.95 -5.31 0.15 -7.12 7.25
Endosome 2.33 -1.06 4.38 3.06 3.34 -10.03 -1.38 6.03 15.06 4.67 -8.42 -1.99 8.07 16.05 5.77
Golgi -0.59 -0.72 2.00 1.59 0.99 -7.72 6.75 -1.11 3.79 -2.58 -7.33 0.00 -0.39 5.14 -0.17
Lipid Particles 1.43 -2.90 1.80 0.46 3.94 -5.75 -1.37 7.48 10.49 7.27 -5.33 -2.93 7.04 10.13 7.50
Mitochondria 1.21 1.30 1.10 -0.91 -1.94 -10.16 3.77 -1.66 9.12 -4.44 -9.17 3.14 -0.25 9.45 -5.00
Mitotic Spindle -2.87 -1.47 -7.51 -3.43 -1.14 -6.65 -2.65 -11.39 -6.76 -5.37 -7.19 -2.97 -13.40 -7.32 -4.95
None -4.08 3.36 -3.62 2.90 -6.03 4.48 8.03 2.39 -2.65 -7.25 2.07 8.51 1.99 -0.46 -9.00
Nuclear Periphery -5.22 -9.75 -18.67 -16.59 0.43 -2.32 -13.09 -24.10 -10.88 -3.68 -2.64 -15.89 -29.80 -13.80 -3.02
Nuclear Periphery Foci -0.30 -2.73 1.19 1.86 3.02 7.36 -6.28 2.81 -5.05 6.45 1.99 -6.45 1.22 -1.44 6.59
Nucleolus -1.78 -1.14 0.55 1.82 1.71 -4.30 3.80 1.19 4.61 -2.20 -4.62 3.41 2.08 5.10 -1.39
Nucleus -7.27 -6.94 -20.95 -9.45 -0.34 -7.90 -4.47 -20.95 -12.24 -5.62 -10.01 -7.32 -28.99 -16.78 -5.12
Peroxisomes -0.82 -0.79 3.40 2.92 1.55 -12.17 -1.46 1.95 12.97 2.17 -12.09 -1.73 3.11 13.30 2.69
Vacuole -1.14 -8.58 -22.09 -20.26 0.19 -7.83 -18.14 -26.00 -20.51 9.02 -7.71 -19.85 -34.44 -28.35 7.80
Vacuole Periphery -0.23 0.13 -1.55 -1.47 -1.58 -5.18 8.37 1.03 5.08 -2.84 -5.14 7.49 -0.40 3.33 -2.94
Standard name
Human Ortholog
Description Cytoplasmic aspartyl aminopeptidase with possible vacuole function; Cvt pathway cargo protein; cleaves unblocked N-terminal acidic amino acids from peptide substrates; forms a 12-subunit homo-oligomer; M18 metalloprotease family
Localization
Cell Percentages cytoplasm (89%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ape4

Ape4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ape4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available