Standard name
Human Ortholog
Description Ornithine carbamoyltransferase; also known as carbamoylphosphate:L-ornithine carbamoyltransferase; catalyzes the biosynthesis of the arginine precursor citrulline
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 1.0 1.0 0.98 0.95 0.87 0.82 0.85 0.77 0.78 0.7 0.99 0.98 0.99 0.99 0.99 1.0 0.96 0.94 0.96 0.94 0.95 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0.07 0.09 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.07 0.08 0.16 0.12 0.21 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 3 2 2 7 1 4 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 8 0 0 0 0 2 0 0 3 3 1 2 3 1 0 0 0 2 2 0 1 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 11 2 2 3 10 7 6 6 5 5 6 1 5 8 11 4 4 0 0 0 0 0 0
Cytoplasm 211 274 140 156 239 185 208 183 140 160 128 121 281 401 468 402 282 249 200 265 128 211 329 336
Endoplasmic Reticulum 0 1 0 0 4 5 16 19 4 9 3 6 0 4 1 11 14 11 0 0 0 0 1 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1
Golgi 0 0 0 0 0 0 0 1 0 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 1 0 0 7 4 5 2 12 5 0 0 0 0 0 1 0 0 1 1 0 1
Nucleus 0 1 0 0 0 0 0 2 3 0 0 2 0 0 0 0 0 0 0 1 0 0 1 1
Nuclear Periphery 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 1 2 9 15 13 33 20 36 3 3 2 1 1 2 0 5 2 2 1 3
Unique Cell Count 212 278 140 156 245 194 240 222 165 209 165 174 284 408 473 407 284 250 209 281 134 224 347 356
Labelled Cell Count 212 295 142 159 247 202 253 232 173 220 172 182 287 417 480 425 301 267 209 281 134 224 347 356


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 82.0 106.7 65.5 54.3 47.5 34.6 28.2 26.9 22.7 20.9 18.8 19.7 68.9 71.7 75.0 124.1 126.3 127.2 64.5 66.3 69.6
Std Deviation (1e-4) 18.0 27.2 17.2 14.3 12.4 9.5 8.8 12.4 8.3 6.1 7.8 8.1 16.3 20.7 19.2 36.2 38.3 40.6 18.2 17.7 18.9
Intensity Change (Log2) – – – -0.27 -0.46 -0.92 -1.22 -1.28 -1.53 -1.65 -1.8 -1.74 0.07 0.13 0.2 0.92 0.95 0.96 -0.02 0.02 0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 1.8 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 -1.9 -2.6 -4.5 -5.3 -4.8 -6.2 -6.0 -7.2 -1.2 -1.6 -1.2 -1.3 -1.0 -0.7
Endoplasmic Reticulum 0 0 0 3.1 3.6 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 3.3 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 3.1 3.4 4.9 4.3 5.7 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 120.8208 167.2229 147.4056 141.926 130.0728 144.3556 119.5157 170.7764 150.2506 156.501 143.3022 149.8046 159.7126 184.975 175.0384 160.7906 165.2286 170.4835
Actin 0.0051 0.0001 0.0008 0 0.0001 0.0002 0.0181 0.0001 0.0054 0.0001 0.0008 0.0001 0.0008 0.0001 0.0001 0.0014 0.0001 0.0002
Bud 0.0007 0 0 0 0.0001 0.0001 0.0007 0 0.0004 0 0.0001 0.0002 0.0001 0.0001 0 0.0007 0 0.0002
Bud Neck 0.0003 0.0001 0.0002 0.0002 0.0004 0.0004 0.0004 0.0001 0.0003 0.0001 0.0003 0.0006 0.0001 0.0002 0.0002 0.0002 0.0002 0.0007
Bud Periphery 0.0007 0 0 0 0 0 0.0005 0 0.0003 0 0.0002 0.0001 0.0001 0 0 0.0009 0 0.0001
Bud Site 0.0005 0 0 0 0.0001 0.0001 0.0018 0 0.0007 0 0.0001 0 0.0002 0.0002 0 0.0005 0 0
Cell Periphery 0.0015 0.0001 0.0001 0 0.0009 0.0002 0.0003 0.0001 0.0002 0 0.0001 0.0015 0.0003 0.0005 0.0001 0.0019 0.0036 0.0005
Cytoplasm 0.9083 0.9967 0.9635 0.9948 0.8743 0.9536 0.8681 0.9979 0.9229 0.996 0.8542 0.9781 0.9683 0.9936 0.9924 0.9696 0.9733 0.98
Cytoplasmic Foci 0.0111 0.0001 0.0045 0.0001 0.0137 0.0146 0.0204 0.0001 0.0201 0.0001 0.0008 0.0003 0.0024 0.0002 0.0002 0.0019 0.0001 0.0002
Eisosomes 0.0008 0 0.0001 0 0.0005 0.0001 0.0002 0 0.0001 0 0.0001 0 0.0001 0 0 0.0003 0 0
Endoplasmic Reticulum 0.0009 0.0001 0.0002 0.0001 0.0008 0.0003 0.0014 0.0001 0.0005 0.0001 0.0002 0.0006 0.0004 0.0003 0.0005 0.0007 0.0004 0.0004
Endosome 0.0018 0 0.0073 0 0.0003 0.0002 0.0137 0 0.0045 0 0.0061 0.0001 0.0006 0 0.0001 0.0003 0 0.0001
Golgi 0.0005 0 0.0012 0 0.0001 0.0001 0.0072 0 0.0039 0 0.003 0 0.0002 0 0 0.0001 0 0
Lipid Particles 0.0044 0 0.0046 0 0.0038 0.0027 0.0117 0 0.0106 0 0.0377 0.0001 0.0012 0.0002 0.0001 0.0019 0.0002 0.0001
Mitochondria 0.0051 0 0.0015 0 0.0048 0.0026 0.0222 0 0.0053 0 0.0847 0.0001 0.0011 0 0.0001 0.0037 0 0.0001
None 0.0092 0.0001 0.0032 0.0001 0.0244 0.0021 0.0038 0.0001 0.0065 0.0001 0.0004 0.0006 0.0024 0.0002 0.0013 0.0024 0.0003 0.0004
Nuclear Periphery 0.0041 0.0005 0.0016 0.0009 0.0186 0.0026 0.0073 0.0003 0.0019 0.0008 0.0011 0.0025 0.0041 0.001 0.0011 0.0014 0.0059 0.0028
Nucleolus 0.0187 0 0.0025 0 0.0291 0.0102 0.0044 0 0.0053 0 0.0004 0.0006 0.0054 0 0.0001 0.0014 0.0005 0.0005
Nucleus 0.0137 0.0017 0.0038 0.0034 0.016 0.0066 0.0076 0.001 0.0041 0.0024 0.0024 0.0081 0.0098 0.0025 0.003 0.0055 0.009 0.0084
Peroxisomes 0.0006 0 0.0016 0 0.0004 0.0003 0.0024 0 0.0019 0 0.0037 0 0.0002 0 0 0.0003 0 0
Punctate Nuclear 0.0018 0 0.0023 0 0.0014 0.0015 0.0052 0 0.0032 0 0.0002 0.0001 0.001 0.0001 0 0.0002 0.0001 0.0001
Vacuole 0.007 0.0003 0.0006 0.0002 0.0053 0.001 0.0018 0.0001 0.0011 0.0001 0.0013 0.0055 0.001 0.0006 0.0006 0.0037 0.0059 0.0036
Vacuole Periphery 0.0033 0 0.0004 0.0001 0.005 0.0006 0.0011 0 0.001 0 0.0023 0.0006 0.0004 0.0001 0.0001 0.001 0.0003 0.0015

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 123.6855 33.9461 29.5077 48.2984 54.8612 207.1127 175.0465 165.4686 165.8367 192.3691
Translational Efficiency 1.7153 2.0721 2.0313 1.24 1.6872 1.8837 1.5448 1.4259 1.4422 1.3544

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1480 1184 1735 1285 1828 1893 217 1348 3308 3077 1952 2633

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 5736.71 6365.82 5836.50 3650.97 6206.59 6529.21 4819.45 2777.80 5996.37 6466.34 5723.44 3203.94
Standard Deviation 1806.88 1640.93 1279.71 1170.41 1651.33 1597.23 989.24 987.37 1738.43 1616.14 1290.97 1165.40
Intensity Change Log 2 — 0.150123 0.024880 -0.651944 — 0.073108 -0.364932 -1.159858 — 0.110614 -0.164542 -0.893587

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000060 0.000091 0.000052 0.000506 0.000025 0.000053 0.000338 0.000694 0.000040 0.000067 0.000084 0.000602
Bud Neck 0.000360 0.000371 0.000389 0.003643 0.000239 0.000485 0.007799 0.003951 0.000293 0.000441 0.001213 0.003800
Bud Site 0.001467 0.001170 0.000907 0.007929 0.000799 0.000931 0.006059 0.021364 0.001098 0.001023 0.001480 0.014807
Cell Periphery 0.003498 0.001951 0.001264 0.001167 0.000797 0.000866 0.003028 0.000393 0.002005 0.001284 0.001460 0.000771
Cytoplasm 0.875236* 0.842511* 0.848829* 0.771351* 0.894543* 0.856696* 0.633260* 0.750439* 0.885905* 0.851238* 0.824864* 0.760645*
Cytoplasmic Foci 0.005995 0.006013 0.003765 0.008902 0.003806 0.004086 0.032382 0.009766 0.004785 0.004827 0.006946 0.009344
Eisosomes 0.000001 0.000001 0.000001 0.000005 0.000000 0.000001 0.000006 0.000006 0.000001 0.000001 0.000001 0.000006
Endoplasmic Reticulum 0.000046 0.000061 0.000015 0.000116 0.000023 0.000022 0.000065 0.000257 0.000033 0.000037 0.000020 0.000188
Endosome 0.000081 0.000056 0.000026 0.003170 0.000050 0.000080 0.001016 0.005452 0.000064 0.000071 0.000136 0.004338
Golgi 0.004492 0.001682 0.000889 0.006440 0.001502 0.001331 0.003093 0.010352 0.002840 0.001466 0.001134 0.008443
Lipid Particles 0.003093 0.001628 0.000878 0.002240 0.001077 0.000632 0.006160 0.001199 0.001979 0.001015 0.001465 0.001707
Mitochondria 0.000116 0.000201 0.000099 0.002362 0.000099 0.000141 0.000166 0.001632 0.000106 0.000164 0.000107 0.001988
Mitotic Spindle 0.000711 0.000274 0.000178 0.026027 0.000514 0.000993 0.003853 0.026594 0.000602 0.000716 0.000586 0.026317
None 0.008535 0.014080 0.007956 0.006478 0.010544 0.015789 0.005462 0.007175 0.009646 0.015131 0.007678 0.006835
Nuclear Periphery 0.000161 0.000113 0.000140 0.000334 0.000090 0.000100 0.000851 0.000579 0.000122 0.000105 0.000219 0.000460
Nuclear Periphery Foci 0.003253 0.001488 0.000784 0.002665 0.001523 0.001268 0.004658 0.003208 0.002297 0.001352 0.001214 0.002943
Nucleolus 0.000057 0.000532 0.000046 0.000181 0.000042 0.000084 0.000194 0.001031 0.000049 0.000256 0.000062 0.000616
Nucleus 0.079366 0.118442 0.125325 0.123694 0.079520 0.111014 0.217796* 0.112681 0.079451 0.113872 0.135604* 0.118056
Peroxisomes 0.000007 0.000131 0.000006 0.000147 0.000008 0.000006 0.000040 0.000381 0.000008 0.000054 0.000010 0.000267
Vacuole 0.010622 0.008201 0.008090 0.031243 0.004235 0.004824 0.073106 0.034785 0.007092 0.006123 0.015318 0.033057
Vacuole Periphery 0.002843 0.001003 0.000362 0.001401 0.000564 0.000600 0.000667 0.008063 0.001584 0.000755 0.000396 0.004811

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.02 -0.99 -4.83 -3.66 -4.66 -2.97 -7.86 -3.71 -3.34 0.32 -2.33 -8.03 -5.38 -4.72 -4.14
Bud Neck -1.70 -3.94 -10.70 -10.39 -9.72 -2.31 -9.55 -10.90 -9.12 6.90 -2.62 -9.42 -15.30 -13.47 -5.85
Bud Site 0.77 1.76 -6.95 -7.45 -7.87 -0.94 -4.22 -7.46 -7.24 -2.61 -0.13 -2.18 -9.73 -9.67 -8.99
Cell Periphery 2.46 4.09 5.84 2.70 3.50 -0.70 -7.81 4.21 4.39 9.53 2.44 0.79 6.63 3.99 7.24
Cytoplasm 6.18 8.38 21.89 16.21 15.52 9.53 22.62 25.21 18.53 -11.23 10.89 17.83 33.62 24.99 12.36
Cytoplasmic Foci -0.66 1.56 -5.90 -5.41 -8.08 -1.53 -11.26 -9.65 -8.28 9.31 -1.25 -7.11 -11.02 -9.99 -1.96
Eisosomes -1.25 -1.67 -7.00 -3.80 -6.43 -1.88 -9.96 -6.75 -6.27 5.01 -1.75 -9.18 -9.51 -7.72 -5.08
Endoplasmic Reticulum -0.31 4.06 -1.62 -0.17 -5.79 -0.20 -5.33 -4.34 -4.28 -2.01 -0.23 2.41 -4.71 -3.33 -5.44
Endosome 1.12 3.85 -3.36 -3.45 -3.54 -1.36 -3.68 -3.95 -3.88 -2.33 -0.57 -2.27 -5.00 -4.96 -4.80
Golgi 4.51 6.28 2.57 -0.44 -1.34 0.66 -2.26 -1.84 -1.98 -0.88 4.30 6.09 -0.01 -1.92 -2.44
Lipid Particles 2.58 3.83 0.20 -3.43 -6.09 1.56 -8.37 -3.37 -6.42 7.24 3.31 -0.27 -2.02 -7.35 -2.14
Mitochondria -0.88 0.24 -1.24 -1.07 -1.24 -1.76 -3.24 -1.94 -1.74 -1.67 -1.59 -1.38 -2.04 -1.81 -2.00
Mitotic Spindle 1.67 1.87 -5.00 -5.28 -5.31 -1.30 -1.25 -6.29 -6.08 -5.17 -0.36 1.05 -8.04 -7.97 -8.19
None -4.33 1.25 5.68 8.35 6.41 -4.20 9.33 6.90 9.67 -1.79 -6.08 4.68 9.02 12.86 6.08
Nuclear Periphery 2.07 -1.82 -8.33 -10.62 -6.88 -1.62 -11.00 -3.88 -3.64 5.81 0.37 -9.19 -6.03 -6.12 -2.00
Nuclear Periphery Foci 3.72 5.29 1.04 -4.67 -8.47 0.50 -7.70 -2.72 -2.97 4.48 3.02 3.02 -1.60 -4.59 -4.76
Nucleolus -1.39 0.74 -4.69 0.88 -4.99 -1.95 -9.40 -3.20 -2.80 -1.85 -1.64 -4.64 -4.12 -0.84 -3.83
Nucleus -8.56 -13.34 -13.86 -5.19 -2.00 -9.18 -14.00 -10.57 -2.40 9.25 -12.56 -18.08 -17.35 -5.66 2.41
Peroxisomes -1.41 0.62 -4.06 0.58 -4.17 0.17 -5.09 -4.49 -4.50 -3.33 -1.40 -1.75 -5.65 -2.12 -5.49
Vacuole 0.01 -1.54 -18.92 -18.95 -18.26 -2.14 -10.83 -20.44 -19.91 4.19 -0.89 -9.95 -27.98 -27.70 -16.87
Vacuole Periphery 2.13 3.20 2.91 0.83 -1.14 -0.20 -0.44 -2.69 -2.61 -2.62 2.12 3.36 -0.31 -2.08 -3.00
Standard name
Human Ortholog
Description Ornithine carbamoyltransferase; also known as carbamoylphosphate:L-ornithine carbamoyltransferase; catalyzes the biosynthesis of the arginine precursor citrulline
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Arg3

Arg3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Arg3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available