Standard name | |
---|---|
Human Ortholog | |
Description | Plasma membrane protein, putative ammonium transporter; regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 8 | 5 | 2 | 0 | 2 | 0 | 2 | 3 | 0 | 8 | 13 | 93 | 135 | 1 | 2 | 3 | 0 | 2 | 4 | 3 | 7 | 12 |
Bud | 0 | 1 | 0 | 0 | 1 | 1 | 6 | 8 | 5 | 8 | 9 | 9 | 2 | 15 | 22 | 0 | 0 | 0 | 2 | 2 | 0 | 1 | 4 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 5 | 1 | 33 | 74 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 3 | 10 | 16 | 24 | 58 | 110 | 202 | 198 | 174 | 232 | 246 | 345 | 23 | 72 | 138 | 20 | 28 | 32 | 2 | 0 | 2 | 8 | 10 | 15 |
Endoplasmic Reticulum | 0 | 3 | 12 | 21 | 11 | 6 | 6 | 3 | 6 | 5 | 7 | 9 | 43 | 142 | 34 | 5 | 4 | 7 | 0 | 2 | 11 | 9 | 26 | 33 |
Endosome | 4 | 1 | 11 | 3 | 9 | 6 | 14 | 3 | 1 | 2 | 3 | 4 | 6 | 1 | 10 | 6 | 11 | 11 | 4 | 11 | 8 | 4 | 7 | 9 |
Golgi | 1 | 0 | 16 | 5 | 13 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 3 | 1 | 1 | 2 | 7 | 12 | 1 | 2 | 4 | 2 | 2 | 3 |
Mitochondria | 7 | 14 | 2 | 1 | 4 | 22 | 18 | 8 | 38 | 24 | 26 | 25 | 0 | 1 | 3 | 3 | 9 | 1 | 1 | 3 | 5 | 6 | 9 | 12 |
Nucleus | 11 | 12 | 39 | 54 | 65 | 30 | 55 | 40 | 21 | 25 | 10 | 9 | 0 | 3 | 0 | 21 | 14 | 14 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 1 | 3 |
Nucleolus | 3 | 3 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 0 | 2 | 55 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
SpindlePole | 0 | 0 | 3 | 1 | 1 | 1 | 1 | 3 | 1 | 0 | 2 | 2 | 1 | 11 | 25 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 46 | 76 | 217 | 154 | 279 | 285 | 357 | 289 | 189 | 206 | 127 | 175 | 27 | 16 | 3 | 45 | 58 | 70 | 90 | 107 | 290 | 134 | 315 | 276 |
Unique Cell Count | 70 | 109 | 279 | 231 | 376 | 375 | 553 | 487 | 366 | 432 | 386 | 504 | 100 | 310 | 362 | 88 | 111 | 132 | 109 | 134 | 333 | 175 | 388 | 386 |
Labelled Cell Count | 76 | 121 | 324 | 269 | 444 | 461 | 663 | 557 | 438 | 507 | 433 | 593 | 120 | 392 | 501 | 104 | 136 | 154 | 109 | 134 | 333 | 175 | 388 | 386 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.5 | 7.5 | 11.3 | 12.3 | 12.6 | 9.3 | 8.7 | 8.3 | 6.9 | 6.8 | 6.7 | 6.6 | 10.4 | 7.9 | 6.8 | 13.5 | 16.3 | 18.6 | 10.9 | 9.7 | 9.5 |
Std Deviation (1e-4) | 3.0 | 1.9 | 2.8 | 3.2 | 3.4 | 3.6 | 3.4 | 3.0 | 2.8 | 2.4 | 2.5 | 1.9 | 2.1 | 1.5 | 1.4 | 5.9 | 7.8 | 8.6 | 2.5 | 2.2 | 2.2 |
Intensity Change (Log2) | – | – | – | 0.12 | 0.16 | -0.28 | -0.37 | -0.45 | -0.72 | -0.73 | -0.76 | -0.77 | -0.12 | -0.52 | -0.73 | 0.26 | 0.53 | 0.72 | -0.05 | -0.21 | -0.24 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.2 | 3.8 | 8.7 | 10.4 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.7 | 4.2 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5.6 | 8.0 | 0 | 0 | 0 |
Cytoplasm | 1.9 | 3.9 | 7.6 | 9.5 | 10.4 | 11.5 | 13.1 | 15.1 | 16.9 | 4.9 | 5.9 | 9.5 | 4.7 | 5.5 | 5.5 |
Endoplasmic Reticulum | 2.2 | -0.9 | -2.1 | -3.0 | -3.5 | -2.0 | -2.7 | -1.9 | -2.1 | 9.4 | 11.4 | 2.5 | 0 | 0 | 0.5 |
Endosome | -1.8 | -1.1 | -1.9 | -1.1 | -3.3 | -3.4 | -3.4 | -2.8 | -3.1 | 0 | -3.1 | -0.8 | 0 | 2.3 | 1.8 |
Golgi | -2.0 | -1.4 | -4.7 | -5.3 | -5.0 | -4.3 | -5.0 | -4.8 | -5.4 | -1.1 | -3.9 | -4.3 | 0 | 0.2 | 1.3 |
Mitochondria | 0 | 0 | 3.5 | 2.3 | 0 | 5.0 | 3.4 | 3.8 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 2.7 | 1.1 | -2.5 | -1.7 | -2.5 | -3.6 | -3.7 | -5.5 | -6.8 | -3.9 | -6.1 | -7.3 | 2.2 | -0.4 | -1.0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6.8 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.0 | 3.6 | 0 | 0 | 0 |
Vacuole | -2.8 | -1.1 | -0.5 | -3.9 | -5.2 | -6.8 | -8.0 | -11.4 | -11.5 | -9.1 | -18.0 | -20.3 | -4.8 | -5.0 | -5.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 12.1171 | 19.6868 | 15.3225 | 14.5622 | 15.5804 | 16.4591 | 7.9523 | 18.8293 | 13.2782 | 12.5198 | 12.3529 | 15.5597 | 8.1714 | 18.1967 | 14.0229 | 12.1194 | 11.8952 | 15.1017 |
Actin | 0.0162 | 0.0001 | 0.0169 | 0 | 0.0004 | 0.0043 | 0.0142 | 0.0092 | 0.0201 | 0.0019 | 0.054 | 0.0006 | 0.0043 | 0.0025 | 0.0227 | 0.0214 | 0.0007 | 0.0008 |
Bud | 0.0006 | 0.0002 | 0.0007 | 0.0005 | 0.0005 | 0.0004 | 0.0009 | 0.0003 | 0.0015 | 0.001 | 0.001 | 0.0007 | 0.0008 | 0.0004 | 0.0006 | 0.0015 | 0.0005 | 0.0005 |
Bud Neck | 0.0029 | 0.0007 | 0.0025 | 0.0014 | 0.0021 | 0.0015 | 0.0023 | 0.0022 | 0.0043 | 0.0013 | 0.0042 | 0.0017 | 0.0032 | 0.001 | 0.0021 | 0.0021 | 0.0014 | 0.0011 |
Bud Periphery | 0.0027 | 0.0024 | 0.0049 | 0.0034 | 0.0043 | 0.0035 | 0.0054 | 0.0036 | 0.0059 | 0.0083 | 0.006 | 0.0046 | 0.0039 | 0.0021 | 0.0027 | 0.0067 | 0.0032 | 0.0036 |
Bud Site | 0.004 | 0.0005 | 0.0019 | 0.0017 | 0.0011 | 0.0007 | 0.0017 | 0.0014 | 0.0144 | 0.0017 | 0.0048 | 0.0013 | 0.0037 | 0.0023 | 0.0051 | 0.0047 | 0.0014 | 0.001 |
Cell Periphery | 0.1662 | 0.3153 | 0.2367 | 0.1992 | 0.1892 | 0.208 | 0.1468 | 0.3809 | 0.2309 | 0.1923 | 0.1413 | 0.2454 | 0.1634 | 0.2922 | 0.232 | 0.2498 | 0.1732 | 0.2211 |
Cytoplasm | 0.0095 | 0.0026 | 0.0037 | 0.0027 | 0.0079 | 0.0024 | 0.0063 | 0.0045 | 0.02 | 0.0022 | 0.0032 | 0.0025 | 0.006 | 0.0025 | 0.0064 | 0.0035 | 0.0021 | 0.0011 |
Cytoplasmic Foci | 0.0096 | 0.0003 | 0.0068 | 0.0006 | 0.0089 | 0.0014 | 0.0156 | 0.0008 | 0.0153 | 0.0012 | 0.0196 | 0.0042 | 0.0173 | 0.0022 | 0.0058 | 0.0278 | 0.0923 | 0.0017 |
Eisosomes | 0.0005 | 0.0001 | 0.0008 | 0 | 0 | 0.0002 | 0.0012 | 0.0052 | 0.0012 | 0.0007 | 0.0009 | 0.0001 | 0.0003 | 0.0001 | 0.0007 | 0.004 | 0 | 0 |
Endoplasmic Reticulum | 0.0241 | 0.0244 | 0.0336 | 0.0067 | 0.0247 | 0.0228 | 0.0093 | 0.0493 | 0.0642 | 0.0096 | 0.0099 | 0.0155 | 0.0107 | 0.0328 | 0.0358 | 0.0149 | 0.0033 | 0.0032 |
Endosome | 0.0628 | 0.0037 | 0.041 | 0.01 | 0.0189 | 0.017 | 0.0299 | 0.0044 | 0.0468 | 0.0156 | 0.0316 | 0.033 | 0.0384 | 0.0065 | 0.0389 | 0.0532 | 0.0177 | 0.0207 |
Golgi | 0.0142 | 0.0003 | 0.0124 | 0.0012 | 0.0024 | 0.0031 | 0.017 | 0.0035 | 0.0354 | 0.0042 | 0.0314 | 0.0138 | 0.008 | 0.0026 | 0.0152 | 0.0143 | 0.0024 | 0.0033 |
Lipid Particles | 0.0883 | 0.0309 | 0.0898 | 0.0556 | 0.0722 | 0.0693 | 0.1185 | 0.0462 | 0.1072 | 0.0776 | 0.1062 | 0.126 | 0.1131 | 0.0462 | 0.1123 | 0.0487 | 0.0131 | 0.0302 |
Mitochondria | 0.0173 | 0.0075 | 0.0138 | 0.0004 | 0.0006 | 0.0037 | 0.0206 | 0.0012 | 0.0389 | 0.1132 | 0.0507 | 0.0057 | 0.0139 | 0.0028 | 0.0244 | 0.0211 | 0.0006 | 0.0056 |
None | 0.0155 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0006 | 0.0538 | 0.0001 | 0.0006 | 0.0002 | 0.0011 | 0.0001 | 0.0006 | 0.0001 | 0.0002 | 0.0008 | 0.0002 | 0 |
Nuclear Periphery | 0.0102 | 0.0031 | 0.0027 | 0.0013 | 0.0066 | 0.0037 | 0.007 | 0.0037 | 0.0037 | 0.0014 | 0.0019 | 0.0014 | 0.0025 | 0.003 | 0.0023 | 0.002 | 0.0011 | 0.0011 |
Nucleolus | 0.0028 | 0.0009 | 0.0014 | 0.0028 | 0.0014 | 0.0016 | 0.0041 | 0.0013 | 0.0024 | 0.0018 | 0.0025 | 0.0013 | 0.0028 | 0.0015 | 0.0014 | 0.0019 | 0.0014 | 0.0019 |
Nucleus | 0.0032 | 0.0011 | 0.0013 | 0.0016 | 0.0038 | 0.0017 | 0.005 | 0.001 | 0.0016 | 0.0014 | 0.0017 | 0.0012 | 0.002 | 0.0013 | 0.0011 | 0.0013 | 0.0014 | 0.0019 |
Peroxisomes | 0.0014 | 0 | 0.0094 | 0.0001 | 0.0007 | 0.0013 | 0.0128 | 0.0001 | 0.0049 | 0.0019 | 0.018 | 0.0003 | 0.0083 | 0.0001 | 0.0015 | 0.032 | 0.0484 | 0.0358 |
Punctate Nuclear | 0.0036 | 0 | 0.0002 | 0 | 0.0007 | 0.0006 | 0.0149 | 0.0001 | 0.0003 | 0 | 0.0005 | 0.0001 | 0.001 | 0 | 0.0002 | 0.003 | 0.0164 | 0 |
Vacuole | 0.5123 | 0.5955 | 0.4939 | 0.6947 | 0.6327 | 0.6335 | 0.4858 | 0.4732 | 0.3662 | 0.5307 | 0.4967 | 0.5292 | 0.5604 | 0.5839 | 0.4692 | 0.4634 | 0.5988 | 0.6366 |
Vacuole Periphery | 0.0321 | 0.0101 | 0.0255 | 0.0158 | 0.0206 | 0.0187 | 0.0269 | 0.0079 | 0.0141 | 0.0316 | 0.0127 | 0.0115 | 0.0356 | 0.0142 | 0.0197 | 0.022 | 0.0203 | 0.0289 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 132.5951 | 110.7886 | 103.4254 | 92.6661 | 67.3839 | 114.5414 | 156.2836 | 128.8533 | 120.7292 | 114.5619 |
Translational Efficiency | 2.409 | 2.4104 | 1.8563 | 1.616 | 2.2513 | 3.0335 | 2.0075 | 1.7063 | 1.6079 | 1.6839 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✔ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1530 | 1135 | 1659 | 172 | 1208 | 1594 | 2368 | 1372 | 2738 | 2729 | 4027 | 1544 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1149.31 | 1204.11 | 1110.94 | 1164.81 | 996.94 | 1130.72 | 1062.36 | 1238.79 | 1082.08 | 1161.24 | 1082.37 | 1230.55 |
Standard Deviation | 227.33 | 286.86 | 158.71 | 216.20 | 178.65 | 205.36 | 153.45 | 325.23 | 220.64 | 245.28 | 157.46 | 315.82 |
Intensity Change Log 2 | — | 0.067199 | -0.048987 | 0.019327 | — | 0.181663 | 0.091694 | 0.313353 | — | 0.121499 | 0.018069 | 0.163379 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000505 | 0.001446 | 0.097769 | 0.015733 | 0.003372 | 0.001352 | 0.057237 | 0.025361 | 0.001770 | 0.001391 | 0.073935 | 0.024288 |
Bud Neck | 0.008288 | 0.012741 | 0.105567 | 0.034157 | 0.027672 | 0.019217 | 0.067223 | 0.035006 | 0.016840 | 0.016524 | 0.083019 | 0.034912 |
Bud Site | 0.008298 | 0.024522 | 0.046875 | 0.065851 | 0.019792 | 0.020307 | 0.040327 | 0.103929* | 0.013369 | 0.022060 | 0.043025 | 0.099687 |
Cell Periphery | 0.187099* | 0.096991 | 0.174159* | 0.044878 | 0.118643* | 0.082082 | 0.061640 | 0.108681* | 0.156896* | 0.088283 | 0.107995* | 0.101573* |
Cytoplasm | 0.000335 | 0.002410 | 0.023476 | 0.022992 | 0.011000 | 0.003621 | 0.022011 | 0.030634 | 0.005041 | 0.003117 | 0.022614 | 0.029783 |
Cytoplasmic Foci | 0.000810 | 0.004950 | 0.004219 | 0.053131 | 0.007965 | 0.003772 | 0.019784 | 0.020352 | 0.003967 | 0.004262 | 0.013372 | 0.024004 |
Eisosomes | 0.000212 | 0.000222 | 0.032580 | 0.000734 | 0.001241 | 0.000264 | 0.009989 | 0.001416 | 0.000666 | 0.000247 | 0.019296 | 0.001340 |
Endoplasmic Reticulum | 0.070075 | 0.033006 | 0.113470* | 0.029695 | 0.096716 | 0.052465 | 0.090168 | 0.096016 | 0.081829 | 0.044372 | 0.099767 | 0.088628 |
Endosome | 0.027296 | 0.067213 | 0.056562 | 0.192231 | 0.063749 | 0.073459 | 0.213844* | 0.138346 | 0.043379 | 0.070861 | 0.149049 | 0.144349 |
Golgi | 0.004390 | 0.012831 | 0.005801 | 0.024153 | 0.009309 | 0.006795 | 0.025311 | 0.038456 | 0.006560 | 0.009305 | 0.017273 | 0.036863 |
Lipid Particles | 0.000683 | 0.001988 | 0.226348* | 0.044603 | 0.009201 | 0.003372 | 0.214906* | 0.079919 | 0.004441 | 0.002796 | 0.219619* | 0.075985 |
Mitochondria | 0.002468 | 0.002496 | 0.001502 | 0.000176 | 0.002413 | 0.004781 | 0.004160 | 0.003410 | 0.002444 | 0.003831 | 0.003065 | 0.003049 |
Mitotic Spindle | 0.000033 | 0.000074 | 0.000082 | 0.001311 | 0.000046 | 0.000165 | 0.000394 | 0.000431 | 0.000039 | 0.000127 | 0.000266 | 0.000529 |
None | 0.000297 | 0.000050 | 0.000039 | 0.000013 | 0.000018 | 0.000046 | 0.000082 | 0.000113 | 0.000174 | 0.000048 | 0.000064 | 0.000102 |
Nuclear Periphery | 0.000198 | 0.000138 | 0.000220 | 0.000210 | 0.000125 | 0.000196 | 0.000199 | 0.000302 | 0.000166 | 0.000172 | 0.000208 | 0.000292 |
Nuclear Periphery Foci | 0.000884 | 0.001133 | 0.001858 | 0.007979 | 0.001189 | 0.000969 | 0.001877 | 0.002528 | 0.001019 | 0.001037 | 0.001869 | 0.003136 |
Nucleolus | 0.000312 | 0.000349 | 0.000115 | 0.000115 | 0.000582 | 0.000570 | 0.000213 | 0.000185 | 0.000431 | 0.000478 | 0.000173 | 0.000177 |
Nucleus | 0.000171 | 0.000264 | 0.001248 | 0.007846 | 0.000617 | 0.000941 | 0.001257 | 0.001340 | 0.000368 | 0.000659 | 0.001253 | 0.002065 |
Peroxisomes | 0.000096 | 0.000307 | 0.027973 | 0.008092 | 0.002883 | 0.000713 | 0.019857 | 0.004640 | 0.001325 | 0.000544 | 0.023200 | 0.005024 |
Vacuole | 0.658596* | 0.682327* | 0.079538 | 0.437436* | 0.604109* | 0.698126* | 0.144308 | 0.290475* | 0.634556* | 0.691555* | 0.117625 | 0.306846* |
Vacuole Periphery | 0.028953 | 0.054542 | 0.000600 | 0.008664 | 0.019358 | 0.026786 | 0.005214 | 0.018459 | 0.024720 | 0.038330 | 0.003313 | 0.017368 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.40 | -20.88 | -2.93 | -2.72 | 12.34 | 2.14 | -16.74 | -8.22 | -9.48 | 8.09 | 0.81 | -26.82 | -9.59 | -9.85 | 14.26 |
Bud Neck | -2.91 | -23.26 | -3.80 | -3.03 | 9.92 | 3.31 | -11.72 | -2.38 | -6.20 | 9.86 | 0.22 | -26.09 | -8.07 | -8.23 | 16.24 |
Bud Site | -6.91 | -14.49 | -5.71 | -3.98 | -1.67 | -0.21 | -7.85 | -16.26 | -16.31 | -12.25 | -5.30 | -16.42 | -18.78 | -16.67 | -12.05 |
Cell Periphery | 12.00 | 1.75 | 13.72 | 5.55 | 12.71 | 5.64 | 9.51 | 1.70 | -3.88 | -7.82 | 13.70 | 9.92 | 9.50 | -1.96 | 1.58 |
Cytoplasm | -3.02 | -15.98 | -5.09 | -4.68 | -0.81 | 4.73 | -6.33 | -7.20 | -10.30 | -3.69 | 2.40 | -16.31 | -10.52 | -11.35 | -3.67 |
Cytoplasmic Foci | -5.42 | -7.15 | -6.47 | -5.96 | -6.06 | 3.49 | -8.02 | -6.99 | -10.85 | -0.52 | -0.45 | -11.77 | -12.08 | -12.02 | -6.41 |
Eisosomes | -0.48 | -17.98 | -2.55 | -2.49 | 17.62 | 4.44 | -11.92 | -0.61 | -10.41 | 12.14 | 4.25 | -21.37 | -4.74 | -10.99 | 20.64 |
Endoplasmic Reticulum | 10.86 | -7.76 | 7.40 | 1.06 | 12.38 | 8.66 | 1.16 | 0.39 | -7.86 | -0.69 | 12.33 | -4.66 | -0.99 | -9.30 | 2.53 |
Endosome | -9.28 | -8.96 | -8.98 | -6.43 | -7.16 | -1.93 | -24.47 | -11.96 | -10.47 | 11.15 | -8.52 | -27.37 | -19.16 | -12.96 | 1.42 |
Golgi | -5.05 | -1.51 | -4.02 | -2.00 | -3.76 | 1.79 | -9.86 | -11.19 | -12.85 | -4.92 | -2.66 | -10.80 | -13.47 | -12.06 | -8.56 |
Lipid Particles | -3.65 | -33.08* | -4.75 | -4.59 | 16.61 | 3.71 | -36.11* | -15.25 | -17.20 | 19.43 | 2.17 | -49.59 | -17.43 | -17.96 | 24.77 |
Mitochondria | -0.05 | 2.05 | 5.07 | 5.65 | 10.32 | -2.40 | -3.67 | -1.66 | 1.35 | 1.36 | -2.17 | -1.74 | -1.12 | 1.16 | 0.11 |
Mitotic Spindle | -3.49 | -5.28 | -2.10 | -2.03 | -2.01 | -1.16 | -1.40 | -4.42 | -2.18 | -0.47 | -1.44 | -1.55 | -4.88 | -3.51 | -1.83 |
None | 1.25 | 1.31 | 1.41 | 2.74 | 2.02 | -1.96 | -2.01 | -4.17 | -2.61 | -0.99 | 1.14 | 0.98 | 0.56 | -2.60 | -1.59 |
Nuclear Periphery | 0.61 | -0.25 | -0.47 | -1.82 | -0.52 | -0.93 | -2.40 | -5.24 | -1.32 | -2.44 | -0.09 | -0.79 | -2.28 | -2.28 | -2.65 |
Nuclear Periphery Foci | -0.74 | -4.79 | -2.85 | -2.75 | -2.50 | 1.55 | -3.82 | -3.83 | -4.36 | -1.78 | -0.13 | -5.81 | -4.97 | -4.83 | -3.07 |
Nucleolus | -0.97 | 9.43 | 6.71 | 5.70 | -0.44 | -0.04 | 1.10 | 1.17 | 1.91 | 0.87 | -0.33 | 1.73 | 1.68 | 2.48 | -0.45 |
Nucleus | -2.50 | -6.41 | -1.89 | -1.87 | -1.69 | -0.96 | -5.14 | -2.14 | -1.41 | -1.03 | -1.45 | -8.88 | -3.06 | -2.63 | -1.95 |
Peroxisomes | -4.45 | -15.28 | -4.30 | -4.18 | 7.78 | 3.08 | -12.89 | -2.00 | -6.90 | 12.14 | 2.46 | -20.90 | -6.00 | -8.29 | 15.99 |
Vacuole | -2.32 | 78.24* | 8.17 | 9.01 | -15.86 | -8.42 | 46.85* | 27.04 | 40.04* | -17.16 | -7.65 | 84.33* | 35.90 | 42.83* | -24.66 |
Vacuole Periphery | -7.68 | 17.31 | 10.26 | 14.78 | -5.85 | -3.63 | 9.69 | 0.64 | 4.57 | -10.64 | -7.27 | 18.94 | 5.01 | 11.58 | -12.78 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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