Standard name
Human Ortholog
Description Alpha subunit of the F1 sector of mitochondrial F1F0 ATP synthase; which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; F1 translationally regulates ATP6 and ATP8 expression to achieve a balanced output of ATP synthase genes encoded in nucleus and mitochondria; phosphorylated; N-terminally propionylated in vivo
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0.07 0.11 0.14 0.25 0.26 0.19 0.25 0.29
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0.06 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0.11 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0.09 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0.05 0.07 0.13 – 0.09 0.06 0.19 0.29 0.17 0.17 0.16 0.17
Mitochondria 0.86 0.98 0.95 0.95 0.9 0.93 0.87 0.84 – 0.92 0.91 0.91 0.85 0.82 0.77 – 0.13 0.06 0.33 0.29 0.25 0.29 0.23 0.22
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0.41 0.14 0.23 0.25 0.29 0.41 0.48 0.45 – 0.39 0.4 0.37 0.23 0.27 0.29 – 0.44 0.61 0 0 0 0.05 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0.05 0 – 0.09 0.06 0.26 0.09 0.26 0.23 0.28 0.25
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0.13 0.06 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.05 0.07 0.08 0.15 0.17 – 0.17 0.12 0.12 0.05 0.05 0.07 – 0.07 0.22 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 3 0 3 0 1 0 – 0 1 3 2 1 2 – 3 2 19 46 125 38 87 120
Bud 0 3 0 1 0 1 0 1 – 0 1 0 0 0 1 – 2 0 0 0 1 0 1 4
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 1 0 1 1 1 1
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 1 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 1 2 – 0 0 4 2 1 0 – 0 1 0 0 1 2 0 0
Cytoplasm 1 0 2 2 2 1 5 10 – 8 6 19 4 2 0 – 5 1 0 0 0 0 0 1
Endoplasmic Reticulum 0 0 0 1 1 1 0 1 – 0 0 2 0 0 0 – 4 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 1 0 0 2 2 3 3 3
Golgi 1 1 3 1 7 0 7 12 – 2 1 4 7 14 25 – 4 1 25 54 84 35 57 69
Mitochondria 104 179 413 352 426 427 689 795 – 531 450 524 110 160 144 – 6 1 44 54 123 57 81 90
Nucleus 0 0 0 2 0 2 4 5 – 3 4 5 0 0 0 – 2 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 1 0 0 0 – 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0
Nucleolus 50 25 102 92 138 190 383 431 – 225 199 211 30 52 55 – 20 11 5 5 12 10 9 10
Peroxisomes 2 2 6 1 4 0 0 1 – 0 0 0 5 9 1 – 4 1 34 16 127 47 99 105
SpindlePole 1 0 0 0 0 0 0 1 – 0 0 0 2 1 1 – 6 1 0 0 0 1 4 0
Vac/Vac Membrane 3 7 19 17 32 35 116 163 – 101 58 69 6 9 13 – 3 4 0 2 1 0 2 3
Unique Cell Count 121 183 436 372 473 458 791 952 580 496 578 130 195 188 45 18 135 187 489 202 354 416
Labelled Cell Count 162 218 548 469 614 657 1206 1422 870 720 841 168 249 243 61 23 135 187 489 202 354 416


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 52.6 68.4 72.6 68.9 74.7 80.7 95.6 108.8 – 112.0 117.1 134.2 56.4 53.8 61.7 – 38.8 59.8 72.3 64.5 71.8
Std Deviation (1e-4) 18.1 24.9 29.7 25.6 26.3 26.6 30.5 37.7 – 40.4 45.0 47.3 24.5 18.5 20.1 – 21.0 29.7 49.3 27.9 34.4
Intensity Change (Log2) – – – -0.08 0.04 0.15 0.4 0.58 – 0.63 0.69 0.89 -0.36 -0.43 -0.23 – -0.9 -0.28 -0.01 -0.17 -0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Cytoplasm 0 0 0 0 0 – 0 0 3.1 0 0 0 – 0 0
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 4.7 7.0 – 0 0
Mitochondria -0.1 -2.6 -0.9 -4.2 -5.8 – -2.0 -2.3 -2.4 -3.8 -5.1 -6.7 – -15.5 -13.1
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Nucleolus 0.4 2.0 5.8 8.6 7.8 – 5.2 5.4 4.5 -0.1 0.9 1.5 – 3.1 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Vacuole 0.1 1.6 2.1 5.5 6.5 – 6.4 4.1 4.2 0.1 0.1 1.3 – 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 69.873 99.6766 89.065 91.8516 91.8363 75.5638 90.6652 124.1193 114.0024 76.229 114.2716 112.0384 113.8389 130.706 123.5748 109.6903 139.5851 114.0451
Actin 0.036 0.0141 0.0176 0.0365 0.0276 0.0022 0.0029 0.0057 0.0011 0.0002 0.0064 0.0079 0.0045 0.0061 0.0123 0.0026 0.0009 0.0012
Bud 0.0002 0.0013 0.0003 0.0081 0.0001 0.0003 0.0002 0.0007 0 0 0.0001 0 0.0003 0.0001 0.0001 0.0001 0 0
Bud Neck 0.0002 0.003 0.0005 0.0153 0.0004 0.0006 0.0074 0.0004 0.001 0 0.0108 0.0001 0.0008 0.0021 0.0007 0.0003 0 0
Bud Periphery 0.0004 0.0014 0.0005 0.006 0.0002 0.0005 0.0002 0.0009 0 0 0.0001 0 0.0011 0.0002 0.0002 0.0001 0.0001 0
Bud Site 0.0008 0.0137 0.0013 0.0181 0.0002 0.0005 0.0061 0.0068 0.0002 0 0.0002 0 0.0014 0.0009 0.0005 0.0004 0.0001 0
Cell Periphery 0.0006 0.0064 0.0032 0.0082 0.0002 0.0014 0.0027 0.0037 0.0004 0 0.0006 0.0001 0.0012 0.0056 0.0013 0.0001 0.0006 0.0001
Cytoplasm 0.0075 0 0.0087 0.0004 0 0.0002 0.0005 0.0001 0 0 0 0 0.001 0.0001 0 0.0003 0 0
Cytoplasmic Foci 0.0202 0.0083 0.0014 0.0075 0.0008 0.0404 0.0064 0.0014 0.0027 0 0.0014 0.0001 0.0063 0.0023 0.0012 0.0046 0.0001 0.0017
Eisosomes 0.0013 0.0133 0.0027 0.0106 0.0029 0.0005 0.011 0.0009 0.0011 0.0002 0.0013 0.0025 0.0039 0.0034 0.0057 0.0008 0.0012 0.0006
Endoplasmic Reticulum 0.0008 0 0.0002 0.0001 0 0.0004 0.0007 0 0 0 0 0 0.0009 0 0 0.0004 0 0
Endosome 0.007 0.001 0.0023 0.0019 0.0002 0.0037 0.0084 0.0009 0.0001 0 0.0001 0 0.0039 0.0025 0.0001 0.0064 0 0.0002
Golgi 0.0127 0.006 0.0208 0.0098 0.0014 0.0039 0.0018 0.0015 0.0005 0 0.0007 0.0005 0.0011 0.0019 0.0007 0.0024 0.0001 0.0029
Lipid Particles 0.0455 0.0568 0.0281 0.0381 0.0315 0.0185 0.045 0.0114 0.0295 0.0002 0.0094 0.0068 0.0213 0.0268 0.0283 0.0264 0.0119 0.022
Mitochondria 0.7905 0.7498 0.808 0.6818 0.8905 0.8384 0.777 0.9065 0.934 0.9961 0.9147 0.9669 0.888 0.8161 0.8822 0.9085 0.9507 0.9437
None 0.0061 0.0001 0.0022 0.0003 0 0.0001 0.0027 0 0 0 0 0 0.0017 0.0001 0 0.0003 0 0.0001
Nuclear Periphery 0.0048 0.0001 0.0012 0.0005 0 0.0194 0.0227 0.0001 0.0001 0 0.0027 0 0.0034 0.0002 0.0004 0.0066 0 0
Nucleolus 0.0137 0.0477 0.0184 0.031 0.0003 0.0411 0.0452 0.0072 0.004 0 0.001 0.0017 0.0147 0.0656 0.027 0.003 0.0008 0.0098
Nucleus 0.0037 0.0033 0.0041 0.0036 0 0.007 0.0037 0.0009 0.0002 0 0.0001 0.0001 0.002 0.0025 0.0016 0.0024 0.0001 0.0003
Peroxisomes 0.0276 0.0325 0.0188 0.0247 0.0409 0.0074 0.0237 0.0167 0.0136 0.0032 0.0316 0.0102 0.0191 0.0165 0.0208 0.0118 0.0051 0.0064
Punctate Nuclear 0.0105 0.0002 0.0005 0.0003 0 0.0002 0.0005 0 0 0 0 0 0.0012 0 0 0.0127 0 0
Vacuole 0.0019 0.0136 0.0157 0.0429 0.0004 0.0031 0.0072 0.0128 0.0007 0 0.0004 0.0001 0.0021 0.0118 0.0018 0.0009 0.0012 0.0004
Vacuole Periphery 0.008 0.0273 0.0436 0.0543 0.0026 0.0103 0.0242 0.0212 0.0105 0 0.0182 0.0028 0.0201 0.0352 0.015 0.0089 0.0268 0.0104

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 331.7623 271.5846 303.4084 303.748 277.7379 337.9746 294.8438 291.7703 341.4991 283.4995
Translational Efficiency 1.373 1.5742 1.4619 1.9046 1.6298 1.6202 1.6882 1.6247 1.5671 1.5557

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
102 732 2003 1134 147 1221 1198 1651 249 1953 3201 2785

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 981.91 700.23 837.65 799.29 619.28 688.39 722.64 808.83 767.83 692.83 794.61 804.95
Standard Deviation 230.01 88.43 103.54 105.48 74.46 85.11 123.33 113.40 238.21 86.56 124.49 110.34
Intensity Change Log 2 — -0.487762 -0.229243 -0.296872 — 0.152634 0.222685 0.385245 — -0.205493 -0.037330 0.006231

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001507 0.000671 0.000645 0.000335 0.000126 0.000590 0.000843 0.001051 0.000692 0.000620 0.000719 0.000760
Bud Neck 0.010023 0.026455 0.006256 0.004742 0.022153 0.052119 0.010580 0.007688 0.017184 0.042500 0.007874 0.006489
Bud Site 0.049662 0.013058 0.010373 0.011092 0.001985 0.014603 0.022604 0.030402 0.021515 0.014024 0.014950 0.022539
Cell Periphery 0.004199 0.000316 0.002731 0.000340 0.000437 0.000458 0.001473 0.001084 0.001978 0.000405 0.002260 0.000781
Cytoplasm 0.211656* 0.434863* 0.533423* 0.533740* 0.378189* 0.382351* 0.487395* 0.508863* 0.309971* 0.402033* 0.516196* 0.518993*
Cytoplasmic Foci 0.082781 0.299345* 0.041103 0.041642 0.339038* 0.289848* 0.043839 0.035329 0.234065* 0.293408* 0.042127 0.037900
Eisosomes 0.000396 0.000441 0.000599 0.000102 0.000614 0.000533 0.000399 0.000120 0.000525 0.000499 0.000524 0.000113
Endoplasmic Reticulum 0.006966 0.000497 0.005078 0.001936 0.000555 0.000943 0.003608 0.002808 0.003181 0.000776 0.004528 0.002453
Endosome 0.028361 0.020521 0.002600 0.004099 0.015003 0.025140 0.002652 0.006727 0.020475 0.023409 0.002619 0.005657
Golgi 0.021962 0.004014 0.000219 0.001056 0.001823 0.008863 0.000429 0.003474 0.010073 0.007045 0.000298 0.002489
Lipid Particles 0.004027 0.006380 0.002445 0.001904 0.020121 0.016752 0.002861 0.001532 0.013529 0.012864 0.002601 0.001683
Mitochondria 0.012649 0.004503 0.000325 0.001115 0.002256 0.015625 0.000668 0.006230 0.006513 0.011456 0.000454 0.004148
Mitotic Spindle 0.008531 0.001289 0.001468 0.004581 0.000171 0.002039 0.002394 0.022736 0.003595 0.001758 0.001814 0.015344
None 0.000304 0.001630 0.002208 0.007129 0.002796 0.001796 0.004591 0.002303 0.001775 0.001734 0.003100 0.004268
Nuclear Periphery 0.004641 0.000153 0.000714 0.001194 0.000484 0.000232 0.000674 0.000867 0.002187 0.000202 0.000699 0.001000
Nuclear Periphery Foci 0.004673 0.000558 0.001301 0.001958 0.000276 0.000227 0.001524 0.000760 0.002077 0.000351 0.001385 0.001248
Nucleolus 0.000568 0.000806 0.000268 0.000472 0.001038 0.001126 0.000476 0.000766 0.000846 0.001006 0.000346 0.000647
Nucleus 0.094825 0.095696 0.313027* 0.255686* 0.158895* 0.105799 0.323726* 0.245667* 0.132650* 0.102013 0.317031* 0.249746*
Peroxisomes 0.002620 0.020562 0.000311 0.002173 0.014442 0.024367 0.000854 0.001377 0.009599 0.022941 0.000514 0.001701
Vacuole 0.426560* 0.067419 0.074711 0.124013 0.038971 0.054907 0.087807 0.118777 0.197742 0.059596 0.079612 0.120909
Vacuole Periphery 0.023088 0.000825 0.000195 0.000688 0.000627 0.001684 0.000602 0.001437 0.009828 0.001362 0.000347 0.001132

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.35 1.48 2.16 2.44 2.53 -3.29 -4.30 -4.79 -1.27 0.11 0.04 -0.36 0.03 -0.02 0.66
Bud Neck -4.80 0.56 0.92 9.00 1.39 -5.73 2.58 3.45 13.90 2.26 -7.38 2.91 3.45 16.26 1.98
Bud Site 2.00 2.22 2.28 1.64 0.50 -7.91 -7.11 -9.54 -3.24 -0.76 0.69 0.68 0.10 -1.89 -1.81
Cell Periphery 1.40 0.35 1.42 0.64 5.34 -0.16 -4.36 -0.72 -0.66 1.49 1.37 -0.53 1.18 -0.56 4.40
Cytoplasm -8.68 -12.47 -9.85 -1.82 5.28 -0.32 -3.67 -2.98 -5.61 1.53 -5.30 -10.75 -8.05 -5.63 6.09
Cytoplasmic Foci -16.84 3.35 3.92 30.40 2.57 2.59 15.95 16.57 38.97 3.70 -4.00 13.24 13.82 49.41 4.83
Eisosomes -0.82 -2.02 3.77 3.82 6.02 0.67 1.97 4.45 10.89 3.87 0.08 0.29 5.52 9.49 7.05
Endoplasmic Reticulum 1.95 0.23 1.16 -9.55 3.63 -1.20 -10.37 -4.41 -3.30 2.16 1.69 -1.69 -0.03 -5.59 3.83
Endosome 0.46 5.13 4.93 8.15 -1.58 -2.78 3.85 3.14 11.48 -3.24 -1.88 6.22 5.54 14.15 -3.49
Golgi 1.53 2.01 1.95 4.54 -1.38 -6.52 4.94 -0.32 5.53 -2.52 0.19 2.27 1.88 6.85 -2.96
Lipid Particles -2.77 1.95 2.86 6.82 3.40 0.87 4.89 5.26 9.85 4.63 0.23 5.01 5.44 11.16 6.33
Mitochondria 0.81 1.53 1.45 3.20 -1.57 -7.28 4.64 -1.38 5.53 -2.89 -2.32 1.95 1.01 6.37 -3.23
Mitotic Spindle 1.68 1.64 1.07 -2.32 -2.57 -3.36 -2.91 -6.61 -5.67 -5.46 1.11 1.08 -2.80 -6.10 -6.13
None -5.00 -5.60 -4.53 -3.29 -2.81 0.90 -0.81 0.85 -0.21 2.22 0.04 -1.37 -1.98 -3.09 -0.89
Nuclear Periphery 1.19 0.95 0.71 -6.75 -3.35 2.41 -3.09 -7.12 -18.52 -7.43 1.32 0.78 0.29 -13.31 -6.16
Nuclear Periphery Foci 1.49 1.13 0.82 -4.75 -2.61 0.93 -7.46 -7.20 -9.32 4.35 1.53 0.28 0.45 -7.63 1.14
Nucleolus -1.54 1.14 0.29 2.53 -2.33 -0.30 2.56 1.40 1.89 -0.29 -1.12 3.06 1.42 2.66 -0.85
Nucleus -0.48 -12.70 -9.59 -20.99 6.04 3.49 -10.51 -6.56 -22.46 7.35 2.33 -15.87 -10.99 -30.54 9.61
Peroxisomes -7.60 3.12 0.87 7.88 -1.83 -2.56 4.20 4.14 11.97 -0.70 -5.40 4.52 4.15 14.28 -2.39
Vacuole 12.62 12.01 8.36 -16.35 -14.48 -2.43 -9.48 -18.44 -22.43 -10.99 8.15 6.15 0.98 -27.82 -19.16
Vacuole Periphery 2.01 2.09 2.05 1.79 -1.30 -4.82 0.33 -1.02 2.57 -0.91 1.79 2.11 1.99 3.17 -1.93
Standard name
Human Ortholog
Description Alpha subunit of the F1 sector of mitochondrial F1F0 ATP synthase; which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; F1 translationally regulates ATP6 and ATP8 expression to achieve a balanced output of ATP synthase genes encoded in nucleus and mitochondria; phosphorylated; N-terminally propionylated in vivo
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Atp1

Atp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Atp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available