Standard name
Human Ortholog
Description (SGD) Epsilon subunit of the F1 sector of mitochondrial F1F0 ATP synthase; which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; F1 translationally regulates ATP6 and ATP8 expression to achieve a balanced output of ATP synthase genes encoded in nucleus and mitochondria; phosphorylated
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.05 0.08 0.07 0.1 0.05 0.05 0 0 – 0 0 0 0.12 0.11 0.07 – – 0.25 0.13 0.18 0.16 0.11 0.2 0.11
Bud 0 0 0 0.06 0 0 0 0 – 0 0 0 0 0 0 – – 0.06 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0.06 0 0 0 0 0 0
Cytoplasm 0 0 0 0.06 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Golgi 0.05 0.31 0.07 0.15 0.1 0 0 0 – 0 0 0 0.64 0.57 0.59 – – 0.69 0.23 0.37 0.23 0.28 0.24 0.32
Mitochondria 0.88 0.65 0.85 0.74 0.74 0.86 0.85 0.82 – 0.86 0.8 0.87 0.42 0.35 0.39 – – 0 0.35 0.28 0.27 0.32 0.31 0.3
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0.12 0 0 0 0 0 0
Nucleolus 0.35 0.17 0.2 0.12 0.2 0.28 0.29 0.27 – 0.26 0.28 0.21 0.1 0.1 0.1 – – 0 0 0 0.06 0 0 0
Peroxisomes 0 0 0.06 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0.12 0 0.2 0.11 0.11 0.08
SpindlePole 0.05 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0.09 0 0 0 0 0
Vac/Vac Membrane 0 0.1 0 0 0 0 0 0 – 0 0 0 0.06 0.06 0.17 – – 0 0 0 0 0.05 0 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 12 7 10 8 9 3 5 – 1 2 2 20 17 12 – – 4 9 31 18 18 48 30
Bud 1 0 3 6 3 6 3 1 – 3 1 0 1 2 1 – – 1 0 2 0 1 2 2
Bud Neck 0 0 0 0 1 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 2
Bud Site 0 0 1 0 0 1 1 0 – 1 1 4 0 0 0 – – 0 – – – – – –
Cell Periphery 0 2 1 2 0 0 0 1 – 0 0 0 1 3 0 – – 1 0 0 0 0 0 0
Cytoplasm 0 0 2 6 3 2 1 9 – 0 5 1 3 2 3 – – 0 0 1 1 0 0 0
Endoplasmic Reticulum 0 0 0 0 1 1 0 0 – 0 0 0 0 0 1 – – 0 0 0 0 0 0 0
Endosome 0 1 0 3 8 1 3 1 – 0 1 0 4 3 3 – – 0 1 5 1 6 9 8
Golgi 2 44 7 15 16 2 0 1 – 0 0 2 102 92 105 – – 11 17 62 25 46 57 85
Mitochondria 38 93 82 74 121 155 147 222 – 128 105 102 68 56 69 – – 0 26 47 30 53 75 78
Nucleus 1 0 0 0 0 1 1 2 – 2 3 0 1 1 0 – – 0 0 0 0 0 1 0
Nuclear Periphery 1 0 0 1 2 1 2 3 – 3 2 3 0 2 2 – – 2 0 0 0 0 1 0
Nucleolus 15 24 19 12 33 50 51 74 – 39 37 25 16 16 18 – – 0 2 2 6 6 6 9
Peroxisomes 0 1 6 4 6 3 4 7 – 2 1 2 3 5 6 – – 0 8 7 22 19 25 21
SpindlePole 2 1 0 1 3 2 2 4 – 1 0 0 2 4 1 – – 0 7 1 1 2 2 2
Vac/Vac Membrane 0 14 1 1 2 5 7 3 – 1 5 0 10 10 30 – – 0 0 4 2 7 8 21
Unique Cell Count 43 142 97 100 164 181 173 270 148 131 117 160 161 177 16 76 170 112 168 243 266
Labelled Cell Count 62 192 129 135 207 239 225 333 181 163 141 231 213 251 19 76 170 112 168 243 266


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 30.3 39.9 24.3 22.2 23.8 22.8 23.2 23.5 – 24.1 22.0 26.8 27.0 24.4 26.1 – – 12.9 35.4 35.8 37.5
Std Deviation (1e-4) 15.7 10.7 7.4 7.0 7.6 8.1 11.2 9.0 – 9.0 9.2 11.0 7.0 6.9 9.3 – – 1.7 13.2 12.3 12.7
Intensity Change (Log2) – – – -0.13 -0.03 -0.09 -0.07 -0.05 – -0.01 -0.14 0.14 0.15 0.01 0.1 – – -0.91 0.54 0.56 0.63


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.7 -0.8 -0.8 0 0 – 0 0 0 1.3 0.9 -0.1 – – 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
Cytoplasm 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
Golgi 1.7 0.7 0 0 0 – 0 0 0 8.9 8.0 8.4 – – 0
Mitochondria -1.8 -2.0 0.2 0.1 -0.5 – 0.4 -0.9 0.6 -6.6 -7.8 -7.2 – – -7.0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
Nucleolus -1.5 0.1 1.5 1.8 1.5 – 1.2 1.5 0.3 -2.2 -2.2 -2.2 – – 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
Vacuole 0 0 0 0 0 – 0 0 0 0 0 4.0 – – 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin – – – – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – – – – –
Bud Periphery – – – – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – – – – –
Cytoplasmic Foci – – – – – – – – – – – – – – – – – –
Eisosomes – – – – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – – – – –
Lipid Particles – – – – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – – – – –
None – – – – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – – – – –
Punctate Nuclear – – – – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – – – – –
Vacuole Periphery – – – – – – – – – – – – – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 134.2767 62.6271 120.0526 169.6769 147.6028 93.3582 110.8138 137.1183 147.797 157.3644
Translational Efficiency 1.7627 2.9498 1.7786 1.1263 1.8525 3.5736 1.9361 1.9326 2.0679 2.0693

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization –
Gene Expression ✔
Translational Efficiency ✘


Micrographs

Cell Count

Number of cells analysed for each strain. R1, R2: biological replicates; R1 & R2: both replicates combined.

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
43 58 N/A N/A N/A 68 6 N/A 43 126 6 0

Protein Abundance

Mean and standard deviation of cell GFP intensity, and intensity change (log2) of each mutant relative to wild type.

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 301.23 309.51 N/A N/A N/A 307.02 359.94 N/A 301.23 308.17 359.94 —
Standard Deviation 11.84 8.68 N/A N/A N/A 6.93 2.38 N/A 11.84 7.89 2.38 —
Intensity Change Log 2 — 0.039121 N/A N/A N/A — — N/A — — — —

Localization Score (DeepLoc)

Localization score for each compartment. Values highlighted in green denote significant localization scores (marked * in the plot).

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000043 0.000032 N/A N/A N/A 0.000030 0.000074 N/A 0.000043 0.000031 0.000074 N/A
Bud Neck 0.000012 0.000013 N/A N/A N/A 0.000024 0.000004 N/A 0.000012 0.000019 0.000004 N/A
Bud Site 0.000028 0.000037 N/A N/A N/A 0.000039 0.000022 N/A 0.000028 0.000038 0.000022 N/A
Cell Periphery 0.000089 0.000076 N/A N/A N/A 0.000107 0.000389 N/A 0.000089 0.000093 0.000389 N/A
Cytoplasm 0.000038 0.000050 N/A N/A N/A 0.000078 0.000027 N/A 0.000038 0.000065 0.000027 N/A
Cytoplasmic Foci 0.000018 0.000021 N/A N/A N/A 0.000020 0.000010 N/A 0.000018 0.000020 0.000010 N/A
Eisosomes 0.000171 0.000045 N/A N/A N/A 0.000062 0.000647 N/A 0.000171 0.000055 0.000647 N/A
Endoplasmic Reticulum 0.000001 0.000001 N/A N/A N/A 0.000001 0.000000 N/A 0.000001 0.000001 0.000000 N/A
Endosome 0.000001 0.000002 N/A N/A N/A 0.000003 0.000000 N/A 0.000001 0.000002 0.000000 N/A
Golgi 0.000005 0.000006 N/A N/A N/A 0.000008 0.000002 N/A 0.000005 0.000007 0.000002 N/A
Lipid Particles 0.000028 0.000036 N/A N/A N/A 0.000046 0.000006 N/A 0.000028 0.000042 0.000006 N/A
Mitochondria 0.000011 0.000013 N/A N/A N/A 0.000016 0.000009 N/A 0.000011 0.000015 0.000009 N/A
Mitotic Spindle 0.000284 0.000417 N/A N/A N/A 0.000355 0.000158 N/A 0.000284 0.000384 0.000158 N/A
None 0.999075* 0.999057* N/A N/A N/A 0.998898* 0.998459* N/A 0.999075* 0.998971* 0.998459* N/A
Nuclear Periphery 0.000000 0.000000 N/A N/A N/A 0.000000 0.000000 N/A 0.000000 0.000000 0.000000 N/A
Nuclear Periphery Foci 0.000034 0.000055 N/A N/A N/A 0.000123 0.000001 N/A 0.000034 0.000092 0.000001 N/A
Nucleolus 0.000028 0.000044 N/A N/A N/A 0.000076 0.000001 N/A 0.000028 0.000061 0.000001 N/A
Nucleus 0.000011 0.000014 N/A N/A N/A 0.000016 0.000004 N/A 0.000011 0.000015 0.000004 N/A
Peroxisomes 0.000124 0.000078 N/A N/A N/A 0.000091 0.000187 N/A 0.000124 0.000085 0.000187 N/A
Vacuole 0.000001 0.000002 N/A N/A N/A 0.000004 0.000000 N/A 0.000001 0.000003 0.000000 N/A
Vacuole Periphery 0.000000 0.000000 N/A N/A N/A 0.000000 0.000000 N/A 0.000000 0.000000 0.000000 N/A

Localization Changes (T-score)

Change in localization of each mutant relative to its control (e.g. UBP2_WT: ubp2Δ vs wild type). Values highlighted in yellow (increase) or blue (decrease) denote significant changes (marked * in the plot).

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Bud Neck N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Bud Site N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Cell Periphery N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Cytoplasm N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Cytoplasmic Foci N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Eisosomes N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Endoplasmic Reticulum N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Endosome N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Golgi N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Lipid Particles N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Mitochondria N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Mitotic Spindle N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
None N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Nuclear Periphery N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Nuclear Periphery Foci N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Nucleolus N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Nucleus N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Peroxisomes N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Vacuole N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Vacuole Periphery N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A

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Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Atp15-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available