Standard name
Human Ortholog
Description Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0.08 0.05 0.07 0.09 0.13
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 – 0.07 0.07 0.11 0.09 0.08 0 0 0 – – – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0.06 0.07 0.06 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0.1 0.14 0.14 0.18 0.18 0.19
Mitochondria 0.96 0.92 0.97 0.93 0.92 0.91 – 0.82 0.87 0.83 0.86 0.87 0.91 0.89 0.91 – – – 0.7 0.65 0.56 0.49 0.48 0.45
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0.06 0.09 – 0.09 0.11 0 0.06 0.05 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0.07 0 0 0.08 0.07 0.13 – 0.16 0.15 0.13 0.11 0.1 0.07 0.07 0 – – – 0 0 0 0 0 0
Peroxisomes 0.05 0 0 0.14 0.09 0 – 0 0 0 0 0 0.06 0.14 0.09 – – – 0.1 0.05 0.17 0.12 0.13 0.12
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.08 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 6 5 2 2 2 2 – 3 2 3 1 0 6 9 1 – – – 11 22 4 8 25 61
Bud 8 2 1 4 10 3 – 20 17 18 9 13 1 6 12 – – – 0 1 0 1 3 3
Bud Neck 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 – – – 0 0 0 1 0 1
Bud Site 0 1 1 0 0 1 – 3 4 1 4 5 0 1 0 – – – – – – – – –
Cell Periphery 5 5 1 1 0 1 – 13 5 10 4 1 3 0 3 – – – 0 1 0 0 0 0
Cytoplasm 0 3 1 2 4 4 – 41 30 56 29 40 2 7 2 – – – 0 1 0 0 0 0
Endoplasmic Reticulum 1 1 0 0 0 0 – 1 2 0 1 1 3 2 0 – – – 0 0 0 0 0 0
Endosome 5 0 0 2 8 5 – 8 0 2 2 0 5 12 17 – – – 17 6 5 8 16 16
Golgi 2 8 0 0 5 0 – 1 0 0 1 1 5 8 24 – – – 45 37 12 24 50 84
Mitochondria 422 232 74 210 341 328 – 497 375 421 277 419 232 467 501 – – – 316 173 49 64 137 205
Nucleus 1 0 1 0 0 0 – 1 0 2 1 4 0 0 0 – – – 0 0 0 1 0 0
Nuclear Periphery 18 10 1 2 23 32 – 53 46 17 20 24 10 14 15 – – – 3 0 0 0 0 4
Nucleolus 30 8 3 18 26 46 – 100 63 66 36 49 19 36 16 – – – 0 0 0 0 2 3
Peroxisomes 20 9 0 32 35 12 – 17 7 12 8 16 16 75 52 – – – 42 14 15 16 37 55
SpindlePole 3 0 0 0 3 2 – 6 4 5 4 5 1 4 7 – – – 2 1 0 0 3 4
Vac/Vac Membrane 15 11 6 1 6 4 – 8 5 6 8 3 9 15 18 – – – 8 5 0 3 5 9
Unique Cell Count 441 252 76 226 372 359 609 432 506 321 480 254 526 552 451 268 89 133 286 454
Labelled Cell Count 536 295 91 274 463 440 772 560 620 405 581 312 656 668 451 268 89 133 286 454


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.4 14.2 8.4 11.4 13.2 12.7 – 12.7 12.9 14.1 14.2 16.1 14.9 15.5 14.9 – – – 13.4 15.4 16.4
Std Deviation (1e-4) 2.6 3.7 2.1 2.8 3.2 3.4 – 4.3 5.0 8.8 6.7 12.3 3.6 4.3 3.8 – – – 3.7 4.5 9.2
Intensity Change (Log2) – – – 0.44 0.65 0.59 – 0.58 0.62 0.74 0.75 0.93 0.82 0.87 0.82 – – – 0.67 0.87 0.96


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Bud 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Cytoplasm 0 0 0 – 0 0 2.7 2.3 2.2 0 0 0 – – –
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Mitochondria -1.4 -1.7 -1.8 – -3.5 -2.7 -3.2 -2.7 -2.6 -1.8 -2.3 -1.9 – – –
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Nuclear Periphery 0 0 2.3 – 2.3 2.6 0 0 0 0 0 0 – – –
Nucleolus 1.2 0 2.2 – 2.9 2.5 2.3 1.9 1.7 1.1 0 0 – – –
Peroxisomes 3.5 2.8 0 – 0 0 0 0 0 0 3.5 2.8 – – –
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 18.7315 19.9196 19.124 18.2496 16.8694 19.6204 23.6151 24.8228 24.9633 22.2478 24.3343 24.1675 25.9897 28.7753 26.8302 21.2972 21.0006 27.5219
Actin 0.0268 0.0233 0.0236 0.021 0.0768 0.004 0.0266 0.013 0.0254 0.0444 0.0023 0.008 0.0155 0.0128 0.0146 0.0036 0.0226 0.0022
Bud 0.0009 0.0003 0.0005 0.0001 0.0007 0 0.0006 0.0001 0.0003 0.0003 0 0 0.0001 0.0001 0.0001 0 0.0008 0
Bud Neck 0.0008 0.0002 0.0009 0 0.0222 0 0.0017 0.0001 0.0009 0.0002 0.0001 0.0002 0.0003 0.0001 0.0001 0.0001 0.0005 0
Bud Periphery 0.0016 0.0004 0.0008 0.0001 0.0012 0 0.0008 0.0001 0.0004 0.0005 0.0001 0.0001 0.0003 0.0002 0.0001 0 0.0012 0
Bud Site 0.0077 0.0007 0.0017 0.0003 0.0105 0 0.004 0.0034 0.0017 0.0005 0 0.0001 0.0008 0.0003 0.0003 0.0001 0.0036 0
Cell Periphery 0.0003 0.0007 0.0004 0.0001 0.0005 0.0001 0.0005 0.0003 0.0002 0.0007 0.0002 0.0001 0.0005 0.0008 0.0001 0.0001 0.0002 0.0001
Cytoplasm 0.0064 0 0.0015 0 0.0006 0 0.0012 0 0.0001 0.0001 0 0.0004 0.0003 0 0 0 0.0023 0
Cytoplasmic Foci 0.0273 0.0071 0.008 0.0084 0.0053 0.001 0.0125 0.0039 0.0021 0.0033 0.0021 0.0067 0.0074 0.006 0.0029 0.003 0.0196 0.0004
Eisosomes 0.0006 0.0007 0.0012 0.0003 0.0008 0.0006 0.0007 0.0006 0.0015 0.0003 0.0002 0.0006 0.0005 0.0006 0.0008 0.0003 0.0009 0.0005
Endoplasmic Reticulum 0.0026 0 0.0015 0 0.0018 0 0.0005 0 0.0005 0.0001 0 0.0012 0.0003 0 0 0 0.0011 0
Endosome 0.0205 0.0069 0.0058 0.0006 0.0093 0.0012 0.0096 0.0025 0.004 0.0092 0.0129 0.008 0.0045 0.0048 0.0025 0.0083 0.023 0.0009
Golgi 0.0286 0.0462 0.022 0.0022 0.0306 0.0138 0.0156 0.0209 0.032 0.0348 0.0102 0.0273 0.0114 0.0276 0.022 0.0262 0.0541 0.0204
Lipid Particles 0.084 0.083 0.0882 0.0307 0.0187 0.056 0.0448 0.0408 0.0249 0.0369 0.0205 0.0176 0.0815 0.0667 0.0478 0.0815 0.0566 0.0172
Mitochondria 0.7156 0.7789 0.7824 0.8934 0.7377 0.8742 0.8119 0.8623 0.8782 0.7848 0.8868 0.902 0.8025 0.8033 0.8506 0.7537 0.7011 0.9221
None 0.0068 0 0.0007 0.0002 0.0011 0 0.0014 0 0.0001 0 0 0.001 0.0007 0 0 0 0.0029 0
Nuclear Periphery 0.0077 0.0001 0.0012 0 0.0058 0.0002 0.0032 0.0001 0.0012 0.0001 0.0001 0.0124 0.0025 0.0002 0 0.0001 0.0053 0
Nucleolus 0.0037 0.001 0.0008 0.0015 0.0139 0.0004 0.002 0.0003 0.0002 0.0008 0.0005 0.0009 0.0178 0.004 0.0005 0.0004 0.0016 0.0004
Nucleus 0.0026 0.0002 0.0005 0.0001 0.002 0.0001 0.003 0.0001 0.0001 0.0002 0.0001 0.0011 0.0013 0.0005 0.0001 0.0001 0.0032 0.0001
Peroxisomes 0.0356 0.0253 0.0462 0.0371 0.0517 0.0389 0.036 0.0275 0.0186 0.0211 0.0052 0.0054 0.0281 0.0375 0.0436 0.0867 0.0816 0.0321
Punctate Nuclear 0.0107 0 0.0005 0 0.0035 0 0.0083 0 0.0001 0.0001 0 0.0008 0.0083 0.0029 0 0 0.0146 0
Vacuole 0.0023 0.0074 0.0014 0.0007 0.0014 0.0005 0.0047 0.0034 0.0009 0.015 0.0172 0.0005 0.0036 0.0051 0.0027 0.0053 0.0013 0.0003
Vacuole Periphery 0.0069 0.0177 0.0102 0.0031 0.0038 0.0088 0.0103 0.0204 0.0069 0.0465 0.0414 0.0055 0.0117 0.0263 0.0114 0.0305 0.0019 0.0031

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 68.512 67.6925 47.8388 104.6384 77.1408 61.2663 72.5178 70.3923 74.0626 99.1128
Translational Efficiency 3.3923 2.3467 3.9609 1.2625 3.1722 4.7223 2.8232 2.8392 2.7726 2.6119

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
168 815 180 531 1574 1022 174 1143 1742 1837 354 1674

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1003.21 1309.07 1479.14 1688.65 1162.34 1313.95 1696.27 1460.28 1146.99 1311.78 1585.86 1532.72
Standard Deviation 176.34 319.24 258.57 345.23 288.77 286.20 361.63 261.04 283.82 301.32 331.75 309.24
Intensity Change Log 2 — 0.383919 0.560135 0.751247 — 0.176878 0.545334 0.329213 — 0.276495 0.552209 0.540128

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.015436 0.025383 0.033401 0.023683 0.018637 0.016883 0.019284 0.019460 0.018328 0.020654 0.026462 0.020800
Bud Neck 0.001997 0.002210 0.017229 0.002396 0.001822 0.002406 0.013343 0.001328 0.001839 0.002319 0.015319 0.001667
Bud Site 0.000799 0.002200 0.014644 0.002392 0.001596 0.001464 0.023433 0.002790 0.001520 0.001790 0.018964 0.002664
Cell Periphery 0.000099 0.000122 0.000234 0.000184 0.000314 0.000131 0.000259 0.000194 0.000294 0.000127 0.000246 0.000191
Cytoplasm 0.000767 0.000826 0.001168 0.000371 0.001143 0.000560 0.002153 0.000557 0.001106 0.000678 0.001652 0.000498
Cytoplasmic Foci 0.043771 0.033848 0.097967 0.012996 0.025471 0.032480 0.043076 0.011000 0.027236 0.033087 0.070987 0.011633
Eisosomes 0.000465 0.003180 0.000773 0.001490 0.004216 0.002359 0.001055 0.000885 0.003855 0.002724 0.000912 0.001077
Endoplasmic Reticulum 0.000262 0.000202 0.001106 0.000545 0.000169 0.000234 0.000394 0.000422 0.000178 0.000220 0.000756 0.000461
Endosome 0.020101 0.008078 0.034626 0.003016 0.005699 0.006183 0.024230 0.006261 0.007087 0.007024 0.029516 0.005232
Golgi 0.085131 0.082161 0.095527 0.036953 0.066822 0.072154 0.047567 0.038152 0.068588 0.076594 0.071954 0.037772
Lipid Particles 0.029097 0.018064 0.027047 0.003286 0.023725 0.019099 0.018634 0.002321 0.024243 0.018640 0.022912 0.002627
Mitochondria 0.698956* 0.753069* 0.542113* 0.873075* 0.790982* 0.792484* 0.723251* 0.885366* 0.782107* 0.774997* 0.631147* 0.881467*
Mitotic Spindle 0.005145 0.002303 0.033531 0.002984 0.000318 0.000655 0.011697 0.004949 0.000783 0.001386 0.022799 0.004325
None 0.000596 0.001426 0.001271 0.001768 0.001725 0.001203 0.002484 0.000792 0.001616 0.001302 0.001867 0.001102
Nuclear Periphery 0.000618 0.000234 0.003461 0.000375 0.000147 0.000197 0.002398 0.000222 0.000192 0.000214 0.002939 0.000270
Nuclear Periphery Foci 0.001832 0.000260 0.002311 0.000234 0.000366 0.000373 0.002453 0.000147 0.000507 0.000323 0.002380 0.000175
Nucleolus 0.000490 0.000816 0.001106 0.000261 0.000401 0.000930 0.001427 0.000174 0.000410 0.000880 0.001264 0.000202
Nucleus 0.000142 0.000159 0.001146 0.000050 0.000091 0.000189 0.000295 0.000034 0.000096 0.000176 0.000728 0.000039
Peroxisomes 0.085101 0.060242 0.064762 0.020356 0.053481 0.044691 0.030272 0.017721 0.056531 0.051591 0.047809 0.018557
Vacuole 0.004846 0.002925 0.013684 0.005645 0.001485 0.003496 0.018858 0.003323 0.001809 0.003243 0.016227 0.004059
Vacuole Periphery 0.004347 0.002293 0.012893 0.007940 0.001391 0.001826 0.013437 0.003901 0.001676 0.002033 0.013160 0.005182

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.66 -2.19 -1.32 0.37 1.34 0.67 -0.16 -0.28 -0.82 -0.02 -0.99 -1.94 -0.98 -0.05 1.31
Bud Neck -0.33 -3.31 -0.55 -0.34 3.22 -0.85 -3.85 1.24 1.67 4.03 -1.01 -4.89 0.41 1.48 4.96
Bud Site -1.95 -3.55 -1.81 -0.19 3.07 0.24 -2.69 -1.32 -1.37 2.53 -0.56 -3.91 -1.64 -1.16 3.62
Cell Periphery -1.08 -5.67 -2.12 -1.43 1.16 1.41 0.43 0.95 -1.34 1.54 1.45 0.41 0.90 -1.99 1.91
Cytoplasm -0.46 -2.49 4.15 3.93 5.29 1.74 -1.01 1.71 0.02 1.67 1.41 -0.98 1.98 2.22 2.41
Cytoplasmic Foci 1.80 -5.04 5.79 7.20 8.78 -2.85 -2.70 8.15 8.97 4.93 -2.91 -7.20 9.59 11.73 9.85
Eisosomes -5.10 -2.00 -6.22 3.10 -3.67 3.92 7.00 7.54 4.24 0.53 2.53 7.86 7.27 5.17 -0.81
Endoplasmic Reticulum 1.04 -2.64 -1.05 -1.30 1.40 -1.34 -2.80 -1.53 -1.11 -0.16 -1.22 -3.43 -2.00 -1.70 1.40
Endosome 3.74 -2.42 5.55 4.35 6.08 -0.42 -3.72 -0.58 -0.08 3.61 0.07 -6.14 2.13 2.13 6.72
Golgi 0.24 -0.65 4.06 6.38 4.82 -0.94 2.41 5.88 6.13 1.19 -1.64 -0.43 7.15 8.83 4.64
Lipid Particles 1.60 0.25 3.94 6.28 5.55 1.58 1.09 10.45 7.69 3.87 2.28 0.35 11.08 10.05 6.76
Mitochondria -1.90 4.08 -6.17 -8.17 -11.07 -0.13 2.62 -9.40 -8.18 -6.25 0.72 7.31 -10.92 -11.88 -12.35
Mitotic Spindle 1.44 -3.92 0.91 -0.40 4.22 -1.99 -3.42 -3.50 -3.23 1.88 -1.22 -5.53 -3.42 -2.63 4.49
None -7.39 -4.01 -6.96 -1.88 -2.25 5.08 -2.72 9.11 5.37 6.27 3.32 -1.42 5.09 2.48 4.56
Nuclear Periphery 1.75 -2.44 1.01 -1.39 2.68 -1.15 -4.40 -1.26 -0.33 4.23 -0.56 -4.33 -1.45 -0.99 4.20
Nuclear Periphery Foci 1.66 -0.42 1.68 0.15 2.89 -0.01 -1.80 1.28 2.22 2.01 1.01 -2.72 1.82 1.95 3.30
Nucleolus -0.53 -0.89 0.62 1.08 1.34 -1.91 -2.16 1.59 2.83 2.68 -1.64 -2.09 1.64 2.48 2.64
Nucleus -0.19 -1.03 1.31 1.49 1.12 -1.32 -1.82 1.66 2.35 2.44 -1.38 -1.25 1.79 2.80 1.35
Peroxisomes 1.67 1.14 4.42 6.37 3.90 1.74 3.28 8.60 6.31 1.94 1.09 1.20 9.56 9.27 4.45
Vacuole 0.74 -2.59 -0.28 -1.55 2.81 -2.96 -4.69 -2.31 0.17 4.12 -2.56 -6.57 -2.81 -0.96 5.31
Vacuole Periphery 0.71 -2.05 -1.07 -3.23 1.43 -1.42 -5.78 -4.32 -3.53 4.45 -0.93 -6.17 -4.73 -4.59 4.10
Standard name
Human Ortholog
Description Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis
Localization
Cell Percentages mitochondrion (93%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Atp17

Atp17


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Atp17-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available