Standard name
Human Ortholog
Description Subunit g of the mitochondrial F1F0 ATP synthase; reversibly phosphorylated on two residues; unphosphorylated form is required for dimerization of the ATP synthase complex, which in turn determines oligomerization of the complex and the shape of inner membrane cristae
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0.12 0.08 0.08 0 0.05 0 0 0 0
Bud 0 0 0.06 0.05 0.05 0 0.05 0 0.06 0 0.07 0 0 0 0 0.1 0.1 0.08 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.08 0.13 0.07 0.07 0.06 0.05 0 0 0 0 0 0 0.11 0.19 0.27 0.09 0.07 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Endosome 0.1 0.12 0.06 0.14 0.06 0 0 0 0 0 0 0 0.21 0.29 0.4 0 0.29 0.28 0.24 0.16 0.19 0.19 0.17 0.16
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.14 0 0 0 0 0 0
Mitochondria 0.7 0.66 0.79 0.75 0.82 0.89 0.92 0.92 0.91 0.92 0.95 0.93 0.27 0.15 0.08 0.28 0.16 0.25 0.45 0.55 0.5 0.49 0.48 0.48
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0.06 0.07 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0.06 0.05 0 0.05 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.07 0.07 0.14 0.24 0.11 0 0 0 0 0 0 0 0.16 0.16 0.15 0.05 0.25 0.14 0.12 0.06 0.12 0.11 0.13 0.09
SpindlePole 0.1 0.08 0.15 0.16 0.08 0.05 0.07 0.06 0.05 0.07 0.05 0 0.24 0.26 0.15 0.4 0.35 0.28 0 0 0.05 0.06 0 0
Vac/Vac Membrane 0.06 0.11 0 0 0 0 0 0 0 0 0 0 0.08 0.07 0.09 0 0 0.06 0 0 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 4 0 0 0 2 0 0 0 0 0 0 12 3 3 7 7 3 12 15 4 2 4 6
Bud 9 5 12 11 16 18 27 24 27 19 26 16 4 5 2 6 8 3 2 4 2 2 3 4
Bud Neck 1 0 1 0 0 2 0 0 0 0 0 0 2 2 0 0 1 2 5 5 4 1 4 3
Bud Site 2 1 5 1 3 18 18 7 9 10 10 13 2 0 0 1 3 1 – – – – – –
Cell Periphery 1 8 3 2 1 1 1 0 1 3 2 1 5 0 0 2 2 1 0 0 0 0 0 1
Cytoplasm 23 34 16 16 19 21 17 13 15 11 6 15 15 30 35 5 6 3 5 3 0 0 3 1
Endoplasmic Reticulum 4 4 3 1 1 7 3 1 1 1 1 3 4 6 4 4 2 1 0 1 0 1 0 1
Endosome 31 33 13 30 19 1 3 4 4 2 0 3 28 47 52 2 24 10 86 52 45 26 36 29
Golgi 0 2 1 0 0 0 0 0 0 0 0 0 5 3 3 3 1 5 11 12 5 2 5 7
Mitochondria 208 179 168 165 262 362 452 500 414 409 361 389 35 24 10 16 13 9 162 178 118 66 99 87
Nucleus 1 2 2 1 7 14 11 6 9 1 5 9 0 1 1 2 0 1 2 1 0 0 0 0
Nuclear Periphery 3 2 1 0 4 15 11 14 29 31 18 24 0 1 0 0 0 0 1 1 1 1 2 2
Nucleolus 2 0 1 1 8 7 20 35 23 18 20 34 0 1 0 0 1 1 1 1 0 0 2 0
Peroxisomes 21 19 29 52 34 12 13 13 9 13 4 10 21 26 19 3 21 5 42 18 29 15 26 17
SpindlePole 31 21 32 35 26 20 34 35 24 33 20 9 32 42 20 23 29 10 8 12 11 8 5 7
Vac/Vac Membrane 19 30 7 5 14 15 9 11 7 9 3 11 11 12 12 1 2 2 10 12 5 3 6 10
Unique Cell Count 297 270 214 221 320 408 491 544 457 446 380 420 132 160 131 58 83 36 360 328 237 138 208 184
Labelled Cell Count 360 344 294 320 414 515 619 663 572 560 476 537 176 203 161 75 120 57 360 328 237 138 208 184


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 4.6 3.9 4.2 4.3 4.0 3.6 3.8 3.8 3.9 3.7 4.3 5.7 5.5 5.5 5.2 5.4 5.4 6.4 6.5 6.7
Std Deviation (1e-4) 0.7 0.8 0.9 1.7 1.5 1.2 1.3 1.2 1.4 1.3 1.1 1.6 1.0 1.3 1.2 0.8 1.0 0.9 1.6 1.9 2.1
Intensity Change (Log2) – – – 0.09 0.13 0.05 -0.1 -0.04 -0.05 -0.01 -0.07 0.13 0.55 0.49 0.49 0.43 0.47 0.48 0.72 0.75 0.79


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.1738 2.7931 2.5326 1.9019 2.2866 2.7173 3.4165 5.4844 4.0578 3.8018 3.7311 4.5912 2.4104 2.6015 1.9405 2.455 1.6116 2.0291
Actin 0.0113 0.0128 0.0092 0.0276 0.0081 0.0081 0.0077 0.0124 0.0094 0.0028 0.0057 0.0052 0.1287 0.0154 0.0153 0.0204 0.0208 0.0104
Bud 0.0005 0.0023 0.0036 0.0004 0.0019 0.0003 0.0003 0.0016 0.0047 0.0002 0.0001 0.0002 0.0019 0.0039 0.0013 0.011 0.0016 0.0004
Bud Neck 0.0025 0.0009 0.0009 0.0005 0.0004 0.0008 0.0002 0.0009 0.001 0.0002 0.0002 0.001 0.0088 0.0017 0.0008 0.0011 0.0055 0.0168
Bud Periphery 0.0011 0.0037 0.0032 0.0008 0.0031 0.0008 0.0004 0.0026 0.0033 0.0004 0.0001 0.0003 0.0022 0.0035 0.001 0.0427 0.0034 0.0007
Bud Site 0.0027 0.021 0.0026 0.0013 0.0012 0.0009 0.0008 0.0059 0.0085 0.0003 0.0003 0.0003 0.0077 0.0378 0.0057 0.0021 0.0043 0.0013
Cell Periphery 0.0003 0.0015 0.0004 0.0002 0.0003 0.0001 0.0001 0.0005 0.0003 0.0001 0 0.0001 0.0002 0.0004 0.0002 0.0009 0.0005 0.0002
Cytoplasm 0.0016 0.0015 0.002 0.0003 0.0082 0.0003 0.0002 0.0009 0.0009 0.0001 0.0001 0.0002 0.0214 0.0096 0.0039 0.0161 0.0034 0.0032
Cytoplasmic Foci 0.041 0.0501 0.0384 0.0243 0.0349 0.0372 0.0371 0.0514 0.0317 0.0194 0.0399 0.0259 0.1074 0.1131 0.1063 0.0823 0.2502 0.0971
Eisosomes 0.0002 0.0004 0.0002 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0004 0.0003 0.0001 0.0003 0.0001 0.0002
Endoplasmic Reticulum 0.0003 0.0004 0.0002 0.0001 0.0006 0.0001 0.0001 0.0002 0.0001 0 0 0.0001 0.0027 0.0013 0.0007 0.0032 0.0025 0.0009
Endosome 0.0613 0.0775 0.0599 0.0689 0.0935 0.029 0.0628 0.0571 0.0331 0.0165 0.0131 0.0266 0.1017 0.1109 0.0964 0.1396 0.0967 0.0983
Golgi 0.058 0.0983 0.0699 0.0941 0.0894 0.0432 0.0701 0.0631 0.0521 0.0228 0.0611 0.0457 0.0623 0.0778 0.0716 0.1269 0.1912 0.0789
Lipid Particles 0.1177 0.0794 0.0731 0.0518 0.059 0.0818 0.0857 0.0586 0.0584 0.0253 0.064 0.0774 0.0537 0.0677 0.0894 0.0419 0.0474 0.0705
Mitochondria 0.4685 0.4329 0.5115 0.6272 0.5247 0.5702 0.4187 0.4885 0.5351 0.5886 0.4023 0.5328 0.2435 0.2958 0.3431 0.1934 0.1608 0.3542
None 0.0048 0.0005 0.0014 0.0001 0.0177 0.0002 0.0002 0.0009 0.0006 0.0001 0.0001 0.0002 0.003 0.0114 0.0014 0.0123 0.0007 0.0027
Nuclear Periphery 0.0033 0.0006 0.0002 0.0001 0.0006 0.0001 0.0002 0.0002 0.0001 0 0 0.0001 0.0265 0.0005 0.0058 0.002 0.0155 0.0013
Nucleolus 0.0024 0.0019 0.002 0.0008 0.0007 0.0005 0.0067 0.0062 0.0015 0.0004 0.0001 0.0002 0.0004 0.0012 0.0012 0.0008 0.0062 0.0007
Nucleus 0.0007 0.0031 0.0007 0.0002 0.0007 0.0001 0.0003 0.0007 0.0002 0.0001 0 0.0001 0.0246 0.0005 0.0022 0.0015 0.0314 0.0014
Peroxisomes 0.2086 0.196 0.2085 0.0947 0.1372 0.2207 0.2999 0.2335 0.2541 0.3206 0.4118 0.281 0.1936 0.234 0.2388 0.2148 0.1207 0.254
Punctate Nuclear 0.0025 0.0006 0.0019 0.0001 0.0004 0.0016 0.0005 0.0004 0.0008 0.0001 0.0002 0.0002 0.004 0.0012 0.0061 0.0006 0.0024 0.0028
Vacuole 0.0054 0.009 0.0063 0.0031 0.0115 0.0014 0.0037 0.0098 0.0023 0.001 0.0003 0.001 0.0025 0.0089 0.0054 0.073 0.0255 0.0021
Vacuole Periphery 0.0052 0.0056 0.0042 0.0032 0.0057 0.0024 0.004 0.0043 0.0017 0.0009 0.0005 0.0015 0.003 0.0029 0.0033 0.013 0.0092 0.0019

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 36.4631 39.6818 16.8261 38.1712 16.4438 9.5068 22.927 40.6197 15.6499 35.9055
Translational Efficiency 2.4803 1.8704 3.9555 1.775 4.3561 8.1511 3.0094 1.5538 3.8001 1.7433

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1532 1669 523 298 1101 1724 178 1269 2633 3393 701 1567

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 654.65 745.68 820.66 853.93 644.56 740.41 903.96 815.53 650.43 743.00 841.81 822.83
Standard Deviation 82.20 118.42 101.27 126.32 117.30 100.85 106.46 123.61 98.54 109.87 108.83 125.04
Intensity Change Log 2 — 0.187833 0.326061 0.383394 — 0.200010 0.487944 0.339423 — 0.193887 0.408644 0.361747

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000320 0.002375 0.002307 0.012574 0.000804 0.002049 0.006068 0.025038 0.000522 0.002209 0.003262 0.022668
Bud Neck 0.031132 0.007371 0.028838 0.018999 0.029582 0.004359 0.033192 0.046713 0.030484 0.005841 0.029944 0.041443
Bud Site 0.012182 0.018961 0.061554 0.068544 0.014339 0.008428 0.053188 0.122536* 0.013084 0.013609 0.059430 0.112268*
Cell Periphery 0.000264 0.000351 0.000239 0.000198 0.000510 0.000225 0.000484 0.001004 0.000367 0.000287 0.000301 0.000851
Cytoplasm 0.098146 0.011703 0.051532 0.039277 0.021985 0.004204 0.017700 0.033222 0.066299 0.007893 0.042941 0.034373
Cytoplasmic Foci 0.325527* 0.079669 0.122737 0.167826 0.105549 0.065498 0.099885 0.067645 0.233542* 0.072468 0.116934 0.086697
Eisosomes 0.000196 0.000105 0.000119 0.000150 0.000273 0.000094 0.000175 0.000208 0.000228 0.000100 0.000133 0.000197
Endoplasmic Reticulum 0.000537 0.000288 0.002594 0.001409 0.000789 0.000205 0.003947 0.001146 0.000642 0.000246 0.002938 0.001196
Endosome 0.079043 0.124765 0.169837 0.131288 0.139978 0.124488 0.140747 0.116486 0.104523 0.124624 0.162450 0.119301
Golgi 0.077217 0.148133* 0.056195 0.104732 0.126249* 0.169338* 0.048136 0.061901 0.097720 0.158907* 0.054149 0.070046
Lipid Particles 0.032295 0.005060 0.009565 0.006876 0.017664 0.004439 0.012132 0.002734 0.026177 0.004745 0.010217 0.003522
Mitochondria 0.209577* 0.464306* 0.127431* 0.115536* 0.415187* 0.515171* 0.144808* 0.241632* 0.295554* 0.490151* 0.131843* 0.217652*
Mitotic Spindle 0.010437 0.024877 0.105614* 0.105150* 0.018692 0.011972 0.115785* 0.045443 0.013889 0.018320 0.108197* 0.056798
None 0.005258 0.001144 0.001814 0.000368 0.006776 0.000320 0.000444 0.000573 0.005893 0.000725 0.001466 0.000534
Nuclear Periphery 0.000308 0.000080 0.001722 0.000404 0.000173 0.000062 0.001359 0.000139 0.000252 0.000071 0.001630 0.000190
Nuclear Periphery Foci 0.000418 0.000214 0.005329 0.000605 0.000666 0.000213 0.005297 0.000425 0.000522 0.000213 0.005320 0.000459
Nucleolus 0.005674 0.000621 0.013469 0.001855 0.007036 0.000761 0.012720 0.002008 0.006244 0.000692 0.013279 0.001979
Nucleus 0.024985 0.003493 0.041899 0.008216 0.008197 0.000599 0.027159 0.011558 0.017965 0.002023 0.038156 0.010923
Peroxisomes 0.056880 0.089267 0.059685 0.140375* 0.063991 0.073665 0.213226* 0.168827* 0.059853 0.081340 0.098673 0.163416*
Vacuole 0.021262 0.011009 0.108574 0.066403 0.012440 0.007374 0.056134 0.044403 0.017573 0.009162 0.095258 0.048586
Vacuole Periphery 0.008341 0.006207 0.028945 0.009213 0.009121 0.006535 0.007414 0.006360 0.008667 0.006374 0.023478 0.006903

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.38 -5.04 -4.37 -3.58 -3.54 -3.18 -4.68 -8.78 -8.31 -6.41 -6.39 -6.51 -9.65 -8.86 -8.26
Bud Neck 10.23 0.60 3.42 -4.66 2.24 7.84 -0.60 -3.86 -13.33 -2.15 12.97 0.16 -3.56 -13.58 -3.14
Bud Site -3.56 -9.59 -8.42 -7.25 -0.98 3.04 -5.33 -19.06 -20.48 -7.87 -0.53 -10.84 -21.12 -20.84 -8.49
Cell Periphery -1.96 0.77 2.47 3.39 1.65 3.63 0.19 -2.15 -3.62 -1.73 1.80 1.02 -2.72 -3.18 -3.01
Cytoplasm 18.83 6.08 8.26 -5.52 1.81 6.84 -0.02 -3.45 -11.82 -2.02 19.84 3.69 8.29 -11.71 2.23
Cytoplasmic Foci 37.56* 24.00 14.97 -9.41 -3.92 8.92 0.88 7.74 -1.10 3.90 34.13 17.79 27.81 -4.57 5.59
Eisosomes 9.97 6.85 2.57 -2.66 -1.45 3.01 1.43 1.07 -9.90 -1.27 5.05 3.39 1.14 -10.10 -5.16
Endoplasmic Reticulum 3.19 -6.68 -3.80 -4.82 3.18 2.54 -2.64 -1.65 -8.24 2.35 3.74 -5.94 -4.28 -9.32 4.50
Endosome -8.60 -9.96 -4.97 -0.43 2.89 2.12 0.11 3.22 1.44 1.64 -4.88 -7.28 -2.76 1.25 5.02
Golgi -13.34 4.21 -2.50 4.62 -4.62 -6.31 10.72 11.13 19.19 -2.53 -14.32 10.07 6.87 20.63 -3.72
Lipid Particles 13.00 9.41 8.25 -0.68 1.12 5.61 1.38 6.33 1.61 2.92 13.68 7.87 14.13 1.17 4.64
Mitochondria -22.94* 7.32 6.80 23.74 0.67 -7.45 13.17 12.54 22.56 -5.15 -22.14* 16.02 7.98 28.50 -7.97
Mitotic Spindle -6.99 -11.05 -7.90 -6.63 -0.04 2.88 -6.49 -7.55 -10.82 4.54 -2.99 -12.60 -12.39 -11.10 6.13
None 4.45 4.15 6.11 1.60 5.17 4.42 4.10 4.23 -1.84 0.26 6.46 5.54 6.95 0.65 4.20
Nuclear Periphery 4.82 -3.14 -1.12 -3.34 2.82 4.25 -3.08 0.85 -4.08 3.14 6.33 -3.99 1.44 -4.67 4.12
Nuclear Periphery Foci 2.55 -3.28 -0.55 -3.00 3.21 4.50 -2.72 1.95 -3.02 2.82 4.58 -4.14 0.99 -4.15 4.22
Nucleolus 7.22 -2.95 3.86 -1.27 4.57 4.57 -1.33 3.48 -2.62 2.73 7.83 -3.06 5.36 -3.17 5.34
Nucleus 10.33 -3.29 6.00 -2.35 6.12 5.64 -3.58 -2.09 -6.78 2.85 12.14 -5.07 3.04 -6.34 6.27
Peroxisomes -6.40 -0.39 -6.63 -3.86 -6.07 -1.80 -7.72 -13.74 -12.47 2.09 -5.93 -5.44 -16.53 -12.54 -7.47
Vacuole 6.42 -14.27 -6.87 -8.21 3.84 4.49 -5.82 -10.81 -12.78 2.02 8.29 -15.56 -11.60 -14.69 8.06
Vacuole Periphery 2.93 -7.63 -0.43 -1.57 6.16 2.68 1.36 2.84 0.27 0.57 3.83 -7.07 2.29 -0.60 7.77
Standard name
Human Ortholog
Description Subunit g of the mitochondrial F1F0 ATP synthase; reversibly phosphorylated on two residues; unphosphorylated form is required for dimerization of the ATP synthase complex, which in turn determines oligomerization of the complex and the shape of inner membrane cristae
Localization
Cell Percentages mitochondrion (69%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Atp20

Atp20


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Atp20-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available