Standard name
Human Ortholog
Description Specific translational activator for the mitochondrial ATP6 mRNA; Atp6p encodes a subunit of F1F0 ATP synthase; localized to the mitochondrial inner membrane
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0.05 0 0 0 0 0.05 0 0 – – – 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 – 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.99 0.98 0.98 0.93 0.96 0.92 0.81 0.85 0.59 0.77 – – – 0.95 0.97 – 0.81 0.86 0.75 0.83 0.91 0.79
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0.06 0.08 – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0.07 0.06 0.23 0.17 0.51 0.28 – – – 0 0 – 0.08 0.05 0.12 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 – 0 0 0 0.06 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 1 0 0 0 0 0 – – – 0 0 – 2 5 3 0 0 0
Bud 0 0 0 1 0 1 2 0 2 4 13 0 – – – 1 1 – 4 1 0 0 0 1
Bud Neck 2 4 5 3 12 8 12 11 6 12 4 9 – – – 5 1 – 1 1 1 0 0 0
Bud Site 0 0 0 0 0 0 0 0 3 0 1 0 – – – 0 0 – – – – – – –
Cell Periphery 6 3 7 3 2 1 2 1 0 0 0 1 – – – 4 1 – 2 5 2 0 0 0
Cytoplasm 402 494 206 215 244 226 399 256 197 210 181 215 – – – 381 247 – 293 421 150 12 23 58
Endoplasmic Reticulum 2 2 2 1 1 0 0 0 5 0 1 0 – – – 25 20 – 1 0 2 0 0 0
Endosome 1 1 0 0 0 0 0 1 1 0 0 1 – – – 6 2 – 4 2 1 0 0 2
Golgi 0 2 0 0 0 1 0 0 0 0 0 0 – – – 0 1 – 3 2 0 0 0 0
Mitochondria 11 4 0 2 0 22 27 18 56 41 155 78 – – – 17 2 – 30 25 24 0 0 1
Nucleus 0 0 0 5 1 1 1 0 0 3 2 4 – – – 0 1 – 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 – 0 0 0 0 0 0
SpindlePole 1 0 1 0 3 2 7 10 7 9 10 4 – – – 0 1 – 3 2 0 0 0 0
Vac/Vac Membrane 0 1 0 0 0 2 1 0 1 2 1 2 – – – 3 3 – 3 4 5 0 0 0
Unique Cell Count 413 504 209 220 249 244 415 278 244 248 306 278 400 254 363 489 201 15 26 74
Labelled Cell Count 426 511 221 230 264 264 452 297 278 281 368 314 442 280 363 489 201 15 26 74


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 3.8 4.8 4.7 4.9 3.9 3.9 4.4 3.4 3.6 3.0 3.3 – – – 5.1 5.1 4.8 – 7.1 6.4
Std Deviation (1e-4) 0.9 0.6 1.1 1.5 1.5 1.6 1.3 1.7 1.0 1.2 0.9 0.9 – – – 1.2 1.1 0.8 – 3.7 3.4
Intensity Change (Log2) – – – -0.01 0.05 -0.28 -0.27 -0.11 -0.5 -0.39 -0.66 -0.53 – – – 0.1 0.11 0.02 – 0.58 0.44


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.1454 -0.6125 -0.5285 -1.2762 -0.2848 -1.0118 -0.4269 -0.511 -0.4842 -0.5518 -0.0234 -0.4072 -1.222 -0.7883 -0.8983 -0.341 -1.7851 -0.8094
Actin 0.0362 0.0015 0.0109 0.0001 0.0088 0.0004 0.0285 0.0003 0.009 0.0241 0.0221 0.0007 0.0286 0.0002 0.0195 0.0426 0.0006 0.0035
Bud 0.0123 0.0066 0.0155 0.0001 0.0019 0.0083 0.0017 0.0009 0.0006 0.0046 0.0026 0.0002 0.0005 0.0065 0.0003 0.001 0 0.0048
Bud Neck 0.0067 0.0003 0.0012 0.0001 0.0013 0.0034 0.0064 0.0003 0.0037 0.0055 0.0006 0.0045 0.001 0.0001 0.0064 0.0043 0.0085 0.0008
Bud Periphery 0.0031 0.0025 0.0116 0.0001 0.0027 0.002 0.0088 0.0012 0.0009 0.0065 0.0122 0.0004 0.0007 0.0011 0.0003 0.0019 0 0.0035
Bud Site 0.0132 0.0091 0.0135 0.0002 0.0122 0.0038 0.0054 0.0077 0.0093 0.0297 0.0012 0 0.0032 0.0026 0.0055 0.0098 0.0001 0.0004
Cell Periphery 0.0005 0.0003 0.0006 0 0.0002 0.0003 0.001 0.0001 0.0002 0.0006 0.0003 0 0.0002 0.0001 0.0002 0.0004 0.0001 0.0001
Cytoplasm 0.012 0.0311 0.0301 0.0097 0.0352 0.0649 0.0127 0.0084 0.0062 0.007 0.0175 0.0079 0.0283 0.0481 0.0606 0.0638 0.0682 0.1494
Cytoplasmic Foci 0.0391 0.009 0.019 0.0005 0.0121 0.014 0.0067 0.0029 0.0031 0.0086 0.0071 0.0026 0.004 0.0048 0.0037 0.008 0.0046 0.0066
Eisosomes 0.0007 0.0004 0.0002 0.0001 0.0002 0.0001 0.0004 0.0002 0.0002 0.0005 0.001 0.0001 0.0003 0.0001 0.0002 0.0006 0.0003 0.0001
Endoplasmic Reticulum 0.0024 0.0006 0.0091 0.0001 0.0038 0.0004 0.0013 0.0002 0.0001 0.0018 0.0009 0.0001 0.0025 0.0004 0.0012 0.0021 0.0044 0.0013
Endosome 0.0235 0.0005 0.0081 0 0.0209 0.0036 0.007 0.0001 0.0008 0.0093 0.0031 0.0001 0.0084 0.0004 0.0045 0.008 0.0035 0.0033
Golgi 0.0073 0.0001 0.0031 0 0.002 0.0005 0.0018 0.0001 0.0014 0.0045 0.0014 0.0001 0.0028 0 0.0012 0.0026 0.0001 0.0016
Lipid Particles 0.0232 0.0007 0.003 0 0.0199 0.0073 0.0047 0.0002 0.0012 0.0123 0.0034 0.0001 0.0039 0.0001 0.0005 0.0075 0.0005 0.0096
Mitochondria 0.0113 0.0011 0.0027 0 0.0041 0.0014 0.0011 0.0002 0.0034 0.003 0.0319 0.0002 0.0014 0.0001 0.0006 0.0049 0.0011 0.0035
None 0.7648 0.9178 0.8438 0.985 0.809 0.8687 0.8798 0.9707 0.9435 0.8375 0.8407 0.9809 0.8932 0.9238 0.8897 0.8298 0.7329 0.8011
Nuclear Periphery 0.0054 0.0032 0.0041 0.0002 0.0258 0.0005 0.0044 0.0003 0.0005 0.0053 0.0039 0.0003 0.0106 0.0004 0.0021 0.0021 0.0168 0.0029
Nucleolus 0.0031 0.0014 0.0012 0.0005 0.0005 0.0021 0.0023 0.0004 0.0004 0.0021 0.0018 0.0001 0.0003 0.0002 0.0002 0.0004 0.0063 0.0004
Nucleus 0.0049 0.004 0.0033 0.0007 0.0097 0.0041 0.0052 0.0017 0.0007 0.0076 0.0035 0.0003 0.0031 0.0091 0.0012 0.0008 0.145 0.0034
Peroxisomes 0.0096 0.001 0.0052 0 0.0194 0.002 0.0142 0.0003 0.0087 0.0198 0.0333 0.0008 0.0029 0.0001 0.0004 0.0056 0.0001 0.0009
Punctate Nuclear 0.0153 0.008 0.0113 0.0024 0.0021 0.011 0.0038 0.0035 0.0059 0.007 0.0027 0.0005 0.0031 0.0015 0.0009 0.0018 0.0052 0.0019
Vacuole 0.0039 0.0005 0.0019 0.0001 0.0058 0.0009 0.0024 0.0002 0.0002 0.0019 0.0057 0.0001 0.0005 0.0002 0.0006 0.0013 0.0014 0.0007
Vacuole Periphery 0.0016 0.0002 0.0007 0 0.0024 0.0003 0.0005 0 0.0001 0.0009 0.0031 0 0.0005 0 0.0003 0.0007 0.0003 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.3964 9.1198 6.0549 11.4326 6.6135 8.0496 13.2249 10.8093 11.8832 13.1884
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
143 183 2891 71 2341 1366 1869 1162 2484 1549 4760 1233

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 618.45 635.69 854.89 775.54 672.14 644.22 770.04 796.24 669.05 643.21 821.57 795.05
Standard Deviation 63.94 85.63 95.47 91.97 71.79 75.60 96.55 124.11 72.45 76.90 104.47 122.58
Intensity Change Log 2 — 0.039666 0.467082 0.326544 — -0.061208 0.196172 0.244442 — -0.011988 0.332347 0.284369

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000029 0.000418 0.000115 0.000218 0.000344 0.001114 0.000056 0.000829 0.000326 0.001031 0.000091 0.000794
Bud Neck 0.007342 0.039845 0.009898 0.018467 0.012254 0.042379 0.002383 0.010487 0.011971 0.042080 0.006947 0.010947
Bud Site 0.000856 0.012175 0.010628 0.006576 0.005039 0.016713 0.003455 0.049446 0.004798 0.016177 0.007811 0.046977
Cell Periphery 0.000154 0.000141 0.000096 0.000331 0.000163 0.000175 0.000236 0.000321 0.000162 0.000171 0.000151 0.000322
Cytoplasm 0.523750* 0.271162* 0.318091* 0.331086* 0.495872* 0.334438* 0.406172* 0.421660* 0.497477* 0.326963* 0.352676* 0.416445*
Cytoplasmic Foci 0.224473* 0.338830* 0.002375 0.053176 0.174246 0.320850* 0.016495 0.020633 0.177137 0.322974* 0.007919 0.022507
Eisosomes 0.000191 0.000223 0.000012 0.000117 0.000124 0.000199 0.000058 0.000051 0.000127 0.000202 0.000030 0.000055
Endoplasmic Reticulum 0.000161 0.001018 0.001247 0.002170 0.000720 0.000463 0.000708 0.001605 0.000688 0.000529 0.001035 0.001637
Endosome 0.005588 0.050916 0.000678 0.004708 0.007366 0.036670 0.001066 0.010386 0.007263 0.038353 0.000830 0.010060
Golgi 0.000474 0.008833 0.000042 0.000165 0.001562 0.009429 0.000052 0.009499 0.001500 0.009359 0.000046 0.008962
Lipid Particles 0.007067 0.010237 0.000310 0.002539 0.007542 0.011568 0.000910 0.001226 0.007515 0.011411 0.000545 0.001302
Mitochondria 0.007248 0.014937 0.000931 0.000306 0.004630 0.025700 0.000435 0.006134 0.004781 0.024428 0.000736 0.005798
Mitotic Spindle 0.000098 0.000964 0.004775 0.000986 0.001440 0.005149 0.003281 0.066349 0.001363 0.004655 0.004188 0.062585
None 0.003259 0.002295 0.004585 0.006273 0.003712 0.002325 0.014362 0.009656 0.003686 0.002321 0.008424 0.009462
Nuclear Periphery 0.000287 0.001786 0.001855 0.000775 0.000719 0.000381 0.000935 0.001431 0.000695 0.000547 0.001494 0.001393
Nuclear Periphery Foci 0.000225 0.000620 0.000202 0.000721 0.000237 0.000324 0.000223 0.000817 0.000237 0.000359 0.000210 0.000812
Nucleolus 0.000906 0.002991 0.000411 0.001134 0.000901 0.003025 0.000663 0.000975 0.000902 0.003021 0.000510 0.000984
Nucleus 0.193140* 0.108180 0.628127* 0.459541* 0.249797* 0.085823 0.515137* 0.262431* 0.246535* 0.088464 0.583762* 0.273782*
Peroxisomes 0.001892 0.051360 0.000269 0.000825 0.004038 0.031130 0.000205 0.006436 0.003915 0.033520 0.000244 0.006113
Vacuole 0.022386 0.075828 0.015153 0.109626 0.028556 0.068251 0.033028 0.110943 0.028201 0.069146 0.022172 0.110867
Vacuole Periphery 0.000473 0.007240 0.000200 0.000260 0.000738 0.003895 0.000141 0.008683 0.000722 0.004290 0.000177 0.008198

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.60 -5.57 -2.19 0.54 -1.35 -1.40 1.32 -0.73 1.18 -5.20 -1.44 1.12 -0.81 1.18 -4.86
Bud Neck -4.75 -1.14 -0.23 3.25 0.12 -11.31 12.03 4.32 13.05 -6.21 -12.10 5.88 3.92 13.66 -0.94
Bud Site -4.81 -7.45 -1.98 2.47 2.07 -6.16 1.45 -6.14 -1.70 -6.91 -6.61 -1.84 -6.22 -1.66 -5.49
Cell Periphery 0.79 4.70 -1.42 -1.66 -2.60 -0.28 -1.04 -0.03 0.19 0.66 -0.21 1.42 -0.14 0.02 -1.07
Cytoplasm 9.37 11.93 7.40 0.31 0.72 18.04 15.46 15.76 -1.15 2.17 20.15 28.58 16.98 -1.44 -2.86
Cytoplasmic Foci -5.25 15.90 11.22 15.95 -3.63 -19.75 39.46 39.15 48.67 -0.15 -20.79 44.26 40.62 51.91 -7.98
Eisosomes -1.10 10.35 2.91 3.49 -6.86 -6.54 12.27 11.10 13.01 -2.27 -6.93 19.44 11.42 13.94 -15.24
Endoplasmic Reticulum -1.61 -19.39 -3.03 -2.29 -1.75 2.14 -4.10 -11.23 -14.84 -8.58 1.31 -8.50 -11.37 -13.00 -6.47
Endosome -8.15 3.87 1.49 8.66 -2.52 -13.08 9.21 3.50 14.74 -6.65 -14.84 9.93 3.64 16.64 -7.38
Golgi -4.61 3.28 2.52 4.82 -2.93 -9.18 6.42 -1.23 5.08 -2.80 -9.94 6.55 -1.21 5.49 -2.81
Lipid Particles -1.33 3.75 2.48 4.95 -4.84 -4.19 12.98 12.02 12.41 -5.86 -4.39 14.17 12.27 13.36 -12.41
Mitochondria -0.98 0.95 1.06 4.24 8.30 -9.19 5.91 2.62 10.50 -4.39 -9.28 5.21 2.83 11.02 -3.54
Mitotic Spindle -3.66 -5.51 -1.80 -0.27 3.36 -3.32 -1.80 -9.29 -8.14 -8.65 -3.30 -3.58 -9.29 -8.22 -8.48
None 1.18 -0.92 -0.89 -1.39 -0.62 3.01 -5.69 -3.62 -5.28 3.21 3.21 -5.58 -3.72 -5.49 0.61
Nuclear Periphery -1.11 -25.19 -6.92 0.18 5.51 1.28 -2.92 -7.62 -22.79 -11.91 0.54 -5.52 -7.86 -12.33 -6.33
Nuclear Periphery Foci -1.43 -0.64 -3.21 -1.62 -3.12 -1.18 -1.37 -8.31 -7.45 -8.74 -1.65 -1.13 -8.82 -7.17 -9.61
Nucleolus -3.39 1.64 0.41 3.67 -1.21 -4.73 4.21 1.12 4.91 -1.23 -5.29 6.41 1.17 5.49 -2.16
Nucleus 4.37 -30.08 -6.38 -8.73 6.10 29.48 -35.51 -9.25 -28.21 18.85 29.28 -61.34 -10.51 -29.19 29.92
Peroxisomes -6.12 4.55 3.25 6.29 -1.59 -12.72 11.07 2.20 12.83 -2.13 -14.01 11.09 2.28 14.10 -2.12
Vacuole -6.90 -0.34 -6.59 -4.71 -6.58 -13.14 -10.02 -24.05 -17.49 -18.83 -14.51 -5.29 -24.94 -18.09 -23.54
Vacuole Periphery -2.71 1.99 2.01 2.84 0.38 -5.30 6.29 -2.54 1.23 -3.38 -5.91 6.18 -2.51 1.82 -3.33
Standard name
Human Ortholog
Description Specific translational activator for the mitochondrial ATP6 mRNA; Atp6p encodes a subunit of F1F0 ATP synthase; localized to the mitochondrial inner membrane
Localization
Cell Percentages cytoplasm (16%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Atp22

Atp22


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Atp22-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available